Molecular Medicine Reports
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2018
·Vol. 19
·期 2
·841-850
·
IF 1.922
Guangwei Liu,Jiangkai Liu,Linping Pian,Songlin Gui,Baoping Lu
α‑lipoic acid (ALA) is a naturally occurring antioxidant with protective effects against various hepatic injuries. The aim of the present study was to investigate the mechanisms by which ALA protects the liver from carbon tetrachloride (CCl4)‑induced liver cirrhosis. The widely used liver cirrhosis rat model was established via an intraperitoneal injection of 2 mg/kg 50% CCl4, three times/week for 8 weeks. Simultaneously, 50 or 100 mg/kg ALA was orally administrated to the rats every day for 8 weeks. The activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) was detected in the serum. The pathological liver injuries were analyzed using hematoxylin and eosin and Masson's trichrome staining. The principal factors involved in the transforming growth factor‑β (TGF‑β)/mothers against decapentaplegic homolog 9 (Smad3) and protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathways and in autophagy were examined using reverse transcription‑quantitative polymerase chain reaction or western blot analysis. The results demonstrated that the administration of ALA alleviated CCl4‑induced liver injury, as demonstrated by decreased ALT and AST activity, improved pathological injuries and reduced collagen deposition. The CCl4‑induced increase in TGF‑β and phosphorylated‑Smad3 expression levels was additionally inhibited by treatment with ALA. Furthermore, the administration of ALA reversed the CCl4‑induced upregulation of light chain 3II and Beclin‑1, and downregulation of p62. The CCl4‑induced suppression of the AKT/mTOR pathway was additionally restored following treatment with ALA. In combination, the results of the present study demonstrated that ALA was able to protect CCl4‑induced liver cirrhosis, an effect that may be associated with inactivation of the TGF‑β/Smad3 pathway and suppression of autophagy.
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CHEMICAL ENGINEERING JOURNAL
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2019
·
IF 8.355
Zhiqiang Sun,Lei Zhao,Caihong Liu,Yufei Zhen,Wenjuan Zhang,Jun Ma
A novel three-dimensional (3D) adsorbent of reduced graphene oxide-β-cyclodextrin (r-GO-CD) aerogel was firstly synthesized through a one-step hydrothermal reaction. Various physicochemical characterization results demonstrated that the cross-linking of “soft” β-cyclodextrin among the “hard” graphene oxide nanosheets, simultaneously combining with self-assembly process of aerogel, contributed to the formation of superior structural and stable r-GO-CD aerogel. The adsorption of bisphenol A (BPA) onto r-GO-CD was chemisorption and endothermic, which fitted well with the pseudo-second-order kinetic and Langmuir isotherm. The saturated adsorption capacity of r-GO-CD towards BPA was 346.0 mg g −1 at 298 K, which was ∼11 times to that of conventional activated carbon. The r-GO-CD displayed a promising environmental application potential as revealed from the stable BPA adsorption efficiency in diverse interferences (H + /OH − , NaCl), presence of trace BPA and repeated cycling tests. The long-term dynamic filtration experiment conducted with raw surface water showed that the effective treated volume of r-GO-CD towards BPA (100 μg L −1 ) was 270 mL which was ∼30 times to that of r-GO due to the excellent selectivity and adsorptivity of β-cyclodextrin for BPA. The distinct adsorption mechanism of r-GO-CD aerogel was demonstrated as synergistic effects of graphene oxide nanosheets and β-cyclodextrin, along with π-π interaction and hydrogen bonding.
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NEW JOURNAL OF CHEMISTRY
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2018
·Vol. 42
·期 5
·3593-3601
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IF 3.201
Ping Shen,Liyan Qiu
In this work, we report a supramolecular polymer based on the host–guest interaction between β-cyclodextrin groups in β-CD-PEG and azobenzene groups in PAE-g-Azo. The supramolecular polymer PAE-g-Azo@β-CD-PEG could self-assemble into a regular nanostructure in aqueous solution due to its proper amphiphilicity. Benefiting from the pH-responsiveness of PAE and the photo-responsive trans-to-cis isomerization of Azo, the supramolecular assemblies apparently show pH and photo responsive properties. Furthermore, the combination of the pH regulation and the irradiation of UV light or visible light induced the recurrent disassembly and reassembly of the supramolecular polymer. This dual-responsive supramolecular polymer has great prospects in various practical applications such as intelligent nanomaterials and nanotechnology.
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PeerJ
·
2018
·
IF 2.118
Yu Lei,Chengkun Wang,Quan Jiang,Xiaoyi Sun,Yongzhong Du,Yaofeng Zhu,Yingmei Lu
The toxicity of engineered nanoparticles remains a concern. The knowledge of biohazards associated with particular nanoparticles is crucial to make this cutting-edge technology more beneficial and safe. Here, we evaluated the toxicity of Ga 2 O 3 nanoparticles (NPs), which are frequently used to enhance the performance of metal catalysts in a variety of catalytic reactions. The potential inflammatory signaling associated with the toxicity of HA/β-Ga 2 O 3 :Cr 3+ NPs in primary cortical neurons was examined. We observed a dose-dependent decrease in cell viability and an increase in apoptosis in neurons following various concentrations (0, 1, 5, 25, 50, 100 µg/ml) of HA/β-Ga 2 O 3 :Cr 3+ NPs treatment. Consistently, constitutively active forms of calcineurin (48 kDa) were significantly elevated in cultured primary cortical neurons, which was consistent with calpain activation indicated by the breakdown products of spectrin. Moreover, HA/β-Ga 2 O 3 :Cr 3+ NPs result in the elevation of LC3-II formation, SQSTM/p62, and Cathepsin B, whereas phosphorylation of CaMKII (Thr286) and Synapsin I (Ser603) were downregulated in the same context. Taken together, these results demonstrate for the first time that calpain activation and a disturbance of autophagy signaling are evoked by exposure to HA/β-Ga 2 O 3 :Cr 3+ NPs, which may contribute to neuronal injury in vitro .
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JOURNAL OF HAZARDOUS MATERIALS
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2018
·
IF 6.434
Lingshuai Kong,Guodong Fang,Ya Kong,Meng Xie,Vinothkumar Natarajan,Dongmei Zhou,Jinhua Zhan
Wastewater systems contain a large number of compounds, such as anthropogenic aromatic pollutants and natural organic matter (NOM), and usually have pH higher than 4. Fenton-like reaction is the most widespread method for removal of organic pollutants, but their reactivity with H 2 O 2 may be inhibited by NOM due to the competition of hydroxyl radicals and chelating agents. In this work, Cu 2 O@β-cyclodextrin was developed to achieve the collaboration between molecular recognition and Fenton-like catalysis to destruct aromatic pollutants at neutral pH. In Cu 2 O@β-CD, covalent Cu O C bond was topotaxially converted from CuCl assisted by β-CD at room temperature. Covalently linked β-CD could keep humic acid from interfering catalytic performance of Cu 2 O surfaces and inhibit the leaching of copper. A higher catalytic ability was observed for Cu 2 O@β-CD with rate constant 0.0331 min −1 than Cu 2 O (0.0064 min −1 ) at neutral pH. A mechanism of synergistic catalysis was proposed on the basis of Cu + , β-CD and phenoxo-Cu 2+ complexes in the Cu 2 O@β-CD/BPA/H 2 O 2 system. The strategy of coupling molecular recognition into Fenton-like reaction provides an efficient and promising approach to the destruction of aromatic pollutants at neutral pH.
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Journal of CO2 Utilization
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2018
·
IF 5.503
Yu Cao,Ran Ma,Ning Wang,Mei-Yan Wang,Xue-Dong Li,Liang-Nian He
Selective hydrogenolysis is a crucial challenge for lignin valorization. A PdCl 2 -catalyzed hydrogenolysis of C OH bond of various lignin β-O-4 alcohol model compounds was developed for preparation of corresponding phenethoxybenzene in 78–98% isolated yield. Notably, the introduction of low-pressure CO 2 (1 MPa) remarkably improves the reaction efficiency and selectivity of phenethoxybenzene. Neither over-reduction of aromatic ring nor further debenzylation was detected. This is understandable that the in situ methylcarbonic acid generated from CO 2 and methanol acts as an acidic catalyst and enhances the leaving ability of hydroxyl group of β-O-4 alcohols. Besides, the present PdCl 2 /CO 2 /MeOH catalyst system proved to be effective for reductive cleavage of C O bonds of β-O-4 ketones, producing ethylbenzenes accompanied with phenols in high yields. Furthermore, this protocol could also be extended to selective hydrodeoxygenation of the carbonyl group to methylene in aromatic ketones. As a sound reaction medium, such in situ CO 2 /MeOH acidic system could be inherently neutralized by depressurizing CO 2 , providing feature advantages for simple post-processing and none waste disposal.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2018
·Vol. 19
·期 3
·683
·
IF 3.687
Quan Gan,Xin Li,Xinwei Zhang,Lanlan Wu,Chongjun Ye,Ying Wang,Junshan Gao,Yan Meng
β-fructofuranosidase (β-FFase) belongs to the glycosyl-hydrolase family 32 (GH32), which can catalyze both the release of β-fructose from β-d-fructofuranoside substrates to hydrolyze sucrose and the synthesis of short-chain fructooligosaccharide (FOS).BmSuc1has been cloned and identified from the silkwormBombyx morias a first animal type of β-FFase encoding gene. It was hypothesized that BmSUC1 plays an important role in the silkworm-mulberry adaptation system. However, there is little information about the enzymatic core sites of BmSUC1. In this study, we mutated three amino acid residues (D63, D181, and E234) that represent important conserved motifs for β-FFase activity in GH32 to alanine respectively by using site-directed mutagenesis. Recombinant proteins of three mutants and wild type BmSUC1 were obtained by using a Bac-to-Bac/BmNPV expression system and BmN cells. Enzymatic activity, kinetic properties, and substrate specificity of the four proteins were analyzed. High Performance Liquid Chromatography (HPLC) was used to compare the hydrolyzing and transfructosylating activities between D181A and wtBmSUC1. Our results revealed that the D63A and E234A mutations lost activity, suggesting that D63 and E234 are key amino acid residues for BmSUC1 to function as an enzyme. The D181A mutation significantly enhanced both hydrolyzing and transfructosylating activities of BmSUC1, indicating that D181 may not be directly involved in catalyzation. The results provide insight into the chemical catalyzation mechanism of BmSUC1 inB. mori. Up-regulated transfructosylating activity of BmSUC1 could provide new ideas for usingB. moriβ-FFase to produce functional FOS.Keywords:Bombyx mori;β-fructofuranosidase;site-directed mutagenesis;hydrolysis;transfructosylation
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Experimental and Therapeutic Medicine
·
2018
·Vol. 16
·期 6
·4951-4958
·
IF 1.41
Lili Zhong,Hong Liu,Weijia Zhang,Xu Liu,Bo Jiang,Hongxin Fei,Zhongren Sun
β‑amyloid (Aβ) aggregation and tau hyperphosphorylation are considered to be the primary pathological hallmarks of Alzheimer's disease (AD). Targeted inhibition of these pathological processes may provide effective treatments for AD. Accumulating evidence has demonstrated that ellagic acid (EA) exerts neuroprotective effects in several diseases. The present study investigated the effects of EA on AD‑associated learning and memory deficits on APP/PS1 double transgenic mice and the underlying mechanisms. APP/PS1 mice or wild‑type C57BL/6 mice were intragastrically administered EA (50 mg/kg/day) or vehicle for 60 consecutive days. The learning and memory abilities of mice were investigated using the Morris water maze test. Hippocampal regions were examined for the presence of amyloid plaques, neuronal apoptosis and tau phosphorylation. Expression levels of APP, Aβ, RAC‑αserine/threonine‑protein kinase and glycogen synthase kinase (GSK)3β in the hippocampus were determined by western blot analysis and ELISA. The results demonstrated that EA treatment ameliorated spatial learning and memory impairment in APP/PS1 mice and significantly reduced neuronal apoptosis and Aβ deposition in the hippocampus (P<0.05 and P<0.01). In addition, EA significantly inhibited the hyperphosphorylation of tau and significantly decreased the activity of glycogen synthase kinase (GSK)3β (P<0.01), which is involved in tau phosphorylation. Overall, these findings indicated that the beneficial effects of EA on AD‑associated cognitive impairments may be attributed to the inhibition of Aβ production and tau hyperphosphorylation, and its beneficial action may be mediated in part, by the RAC‑α serine/threonine‑protein kinase/GSK3β signaling pathway.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2017
·
IF 2.714
Jianxin Chen,Yunping Pu,Chongbin Wang,Jian Han,Yunlong Zhong,Kaili Liu
β-cyclodextrin, a type of molecule with a cavity structure and large number of hydroxyl groups , has great potential in water treatment, attributing to its highly adsorption ability. In this work, silica nanoparticles coated with β-cyclodextrin (SiO 2 @β-CD) as adsorbent have been successfully synthesized via distillation-precipitation polymerization. The composition , morphology and structure of SiO 2 @β-CD nanoparticles were characterized by FTIR and TEM . The adsorption performance of SiO 2 @β-CD nanoparticles were analyzed by the adsorption ability of bromophenol blue (BPB) and crystal violet (CV) in water solution. In addition, the dyes sorption processes have been thoroughly studied with both kinetic and equilibrium models. The results show that the adsorption rates for dyes is fast and the time evolution can be perfectly fitted using a pseudo-second-order kinetic model. The obtained maximum adsorption capacities exhibit an extremely good ability to remove dye at ambient temperature.
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Science China-Materials
·
2025
·
IF 7.4
Zhao Weidong,Zheng Mengfei,Chu Hongyu,Zhang Jingxuan,Wang Kun,Yang Chenguang,Shen Na,Tang Zhaohui
β-Lapachone (β-Lap) is a promising ortho -naphthoquinone drug for cancer treatment and has been in clinical trials. Its application is constrained by the low aqueous solubility, and severe side effects. Even prodrug designation is an effective approach to render it with tumor selectivity, it is limited by the lack of modifiable groups on β-Lap. Herein, a novel azo bond primary cleavage and carbon–carbon (C–C) bond secondary cleavage-based polymeric β-Lap prodrug (Azo-Lap NP) is designed, in which the self-immolated para -aminobenzyl linker is connected to poly( l -glutamic acid) (PGlu) via azo linkage and the responsive drug release of β-Lap against tumors can be achieved under high NAD(P)H:quinone oxidoreductase 1 (NQO1) expression and low pH environment in tumors. The effective covalent loading of β-Lap by Azo-Lap NPs permitted a high administration dose of β-Lap and enabled significant tumor retention time. Moreover, Azo-Lap NPs markedly reduced the side effects of β-Lap by avoiding hemolysis and the production of methemoglobin. The safety of Azo-Lap NPs administration is validated in the antitumor experiment of mice. In the 4T1 model, Azo-Lap NPs exhibited a markedly higher tumor suppression rate than β-Lap. This work provides an effective and safe polymeric prodrug for tumor selective delivery of β-Lap.
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Foods
·
2025
·Vol. 14
·期 7
·1108
·
IF 5.1
Jiaofen Lin,Jian Zeng,Guozong Shi,Zesheng Zhuo,Yanyun Guan,Zhipeng Li,Hui Ni,Peng Fei,Bingqing Huang
In this study, we developed a soybean oil-based emulsion system stabilized by caffeic acid-modified mushroom-derived chitosan, significantly enhancing its functional properties. The modification increased the grafting ratio from 5.02% to 8.26%, which greatly improved antioxidant activity and antimicrobial efficacy against Escherichia coli and Staphylococcus aureus. The modified chitosan exhibited superior rheological properties, including increased viscosity and elasticity, contributing to improved emulsification performance. Emulsions stabilized with caffeic acid-modified chitosan showed smaller and more uniform droplet sizes, along with greater stability, as indicated by a higher zeta potential (55.63 mV). These modifications resulted in enhanced β-carotene encapsulation efficiency (up to 87.46%) and improved bioaccessibility (up to 52.13%), highlighting the system’s potential as an efficient food-grade carrier for hydrophobic bioactive compounds. In conclusion, caffeic acid-modified mushroom chitosan is an effective natural emulsifier, enhancing stability, antioxidant activity, and nutrient delivery, and has promising applications in functional foods and nutraceuticals.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shuo Liu,Hongli Chao,Dajun He,Yi Wang,Yi Yang
Accurate, specific, and cost-effective detection of toxic cyanogenic glycosides is crucial for ensuring biological health and food safety. In this study, a novel biosensor based on co-immobilized multi-enzyme system was constructed by artificial antibody-antigen-directed immobilization for the colorimetric detection of amygdalin through a cascade reaction catalyzed by β-glucosidase, glucose oxidase, and horseradish peroxidase. Artificial antibodies and antigens were prepared using catechol and 3,4-dihydroxybenzaldehyde, respectively, to generate mutual affinity recognition ability for enzyme immobilization. On this basis, the biosensing system showed a complete response to amygdalin within 4 min, with a linear range from 2 to 10 μM, a detection limit of 0.18 μM, and a quantification limit of 0.6 μM. In addition, this sensor had good precision, reproducibility, stability, and reusability. This study proposed a method for detecting cyanogenic glycosides, providing a successful case for the application of cascade biosensors in food safety detection.
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