BIOCHEMICAL ENGINEERING JOURNAL
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2018
·
IF 3.226
Amjad Hussain Memon,Runsheng Ding,Qipeng Yuan,Hao Liang,Yun Wei
Enhancing activity of multiple enzymes by co-localization are key topics in bionanotechnology. Herein, we introduce a facile co-immobilization of glucose oxidase (GOx) and horseradish peroxidase (HRP) in the matrix of Cu (copper) and GMP (guanosine 5′-monophosphate). The multienzyme system by co-immobilizing GOx&HRP observed with high catalytic efficiency and stability compared with free GOx&HRP enzymes. GOx&HRP@Cu/GMP stability was observed 40% increased at pH 3 and thermal stability more than 70% determined at 90 ᵒ C. The storage stability was sustained 8 times higher than free enzymes after thirteen days and reusability after reacting eight times with fresh substrate retains 90% relative activity. Further, GOx&HRP@Cu/GMP demonstrated an excellent selectivity in the development of glucose biosensor and exhibited good resistance against the protease enzymes and organic solvents. The obtained results emphasize that co-immobilization of GOx&HRP in the matrix of Cu/GMP could be an efficient protocol for the biocatalyst encapsulation.
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高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Jian Li,Keman Zuo,Weibing Wu,Zhaoyang Xu,Yonggang Yi,Yi Jing,Hongqi Dai,Guigan Fang
This study describes the preparation of an aerogel adsorbent based on nanofibrillated cellulose(NFC) and polyethyleneimine(PEI) via electrostatic combination without chemical crosslinking. Purely physical crosslinking between NFC and PEI was confirmed by attenuated total refraction-Fourier transform infrared spectroscopy (ATR-FTIR) and thermogravimetric analysis (TGA). The NFC/PEI hybrid aerogels exhibited good structural stability and shape recovery in water. Absorption experiment shows that the hybrid aerogels are excellent adsorbents for the removal of heavy metal ions owing to the highly porous structure and the existence of plenty of amine groups. Thermodynamic and kinetic study showed pseudo-second-order equation and Langmuir model ( R 2 > 0.999) as the best fit. The maximum adsorption capacity of Cu(II) and Pb(II) according to Langmuir model was 175.44 mg/g and 357.44 mg/g, respectively. Besides, the Cu(II) or Pb(II) loaded NFC/PEI aerogel could be easily regenerated by EDTA and retain the adsorption capacity after repeatedly using. This environmentally friendly hybrid aerogels with high-performance are promising candidates for sewage disposal and metal pollution treatment.
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PubMed
高影响因子
CHEMOSPHERE
·
2018
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IF 4.427
Huafeng Li,Jin Wang,Rui Wang,Kaibo Huang,Wanying Luo,Xueqin Tao,Zhi Dang,Hua Yin,Chuling Guo,Guining Lu
Polybrominated diphenyl ethers (PBDEs) belong to a class of persistent organic pollutants (POPs), with potential toxicity to the liver, reproductive system, and development of mammals. The highly toxic and concentrated congener, 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47), was chosen to investigate debromination mechanisms by the two synthetic iron-based bimetals (Pd/Fe 0 and Cu/Fe 0 ) in two protic solvents (water and ethanol). SEM, XPS, and BET analyses showed that the synthetic bimetals Pd/Fe 0 and Cu/Fe 0 were spherical with diameters of about 100 nm and loaded with ∼1% (wt%) of Pd and Cu, respectively. GC-MS was used for the analysis of degradation products and the chromatograms showed that both Pd/Fe 0 and Cu/Fe 0 bimetals had effective reducing properties in water solvent. In ethanol solvent, debromination of BDE-47 by Pd/Fe 0 showed a similar high activity, but BDE-47 could be hardly degraded by Cu/Fe 0 . The dominant debromination products of BDE-47 by Pd/Fe 0 and Cu/Fe 0 were ortho -substituted and para -substituted BDEs, respectively. Active H-atomic transfer was found to play a key role in the debromination of BDE-47 by Pd/Fe 0 in both, water and ethanol, with a preference for para -debromination along with the formation of dibenzo-p-furan (DF) as the by-product, mainly in water. In contrast, electron transfer with a preference for ortho -debromination was found to play a predominant role for Cu/Fe 0 system in water. More importance should be provided to active H-atomic transfer for its high efficiency. In-depth study on the mechanism of formation of by-product DF would be significant for its higher toxicity, possibility of accumulation and migration in the environment.
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高影响因子
Crystals
·
2019
·Vol. 9
·期 5
·255
·
IF 2.061
Niannian Yang,Runkai Wang,Pinhua Rao,Lili Yan,Wenqi Zhang,Jincheng Wang,Fei Chai
Calcium alginate (CA) beads as a green sorbent were easily fabricated in this study using sodium alginate crosslinking with CaCl2, and the crosslinking pathway was the exchange between the sodium ion of α-L-guluronic acid and Ca(II). The experimental study was conducted on Cu(II), Cd(II), Ni(II) and Zn(II) as the model heavy metals and the concentration was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). The characterization and sorption behavior of the CA beads were analyzed in detail via using scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). The adsorption experiments demonstrated that the CA beads exhibited a high removal efficiency for the selective adsorption of Cu(II) from the tetra metallic mixture solution and an excellent adsorption capacity of the heavy metals separately. According to the isotherm studies, the maximum uptake of Cu(II) could reach 107.53 mg/g, which was significantly higher than the other three heavy metal ions in the tetra metallic mixture solution. Additionally, after five cycles of adsorption and desorption, the uptake rate of Cu(II) on CA beads was maintained at 92%. According to the properties mentioned above, this material was assumed to be applied to reduce heavy metal pollution or recover valuable metals from waste water.Keywords:alginate beads;green sorbent;selective adsorption;heavy metals
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CHEMICAL ENGINEERING JOURNAL
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2019
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IF 8.355
Wengao Zeng,Tao Cai,Yutang Liu,Longlu Wang,Wanyue Dong,Hui Chen,Xinnian Xia
Tungsten trioxide (WO 3 ) has a wide application in environmental remediation due to its stability. Yet, the application of WO 3 is severely limited by its wide band gap and weak electron reduction ability. In this work, we intend to enhance the visible light absorption and photocatalytic performance by constructing an organic-inorganic Z-scheme system WO 3 @Cu@PDI. Apparently, WO 3 @Cu@PDI has excellent visible light absorption and photocatalytic degradation ability in the degradation of tetracycline hydrochloride (TC). Under visible light irradiation, the degradation rate of composites is 40 times faster than that of sole WO 3 and 5 times than PDI. The photocatalytic activity of WO 3 @Cu@PDI toward TC still kept 85% after three reuses. We revealed that this high photoactivity arisen from the efficient carriers separation and the formation of Z-scheme junction. Importantly, this Z-scheme electron transfer pathway was confirmed by thorough experimental study. This work demonstrated the significant potential of WO 3 -based inorganic-organic Z-scheme photocatalyst in environmental remediation.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Bing Chang,Fengtao Zhang,Zhaojun Min,Nan Wang,Qingchun Xia,Jing Fan,Maohong Fan,Jianji Wang
Cu-based catalysts, especially co-sites of Cu 0 and Cu Ⅰ , are the best competitors for electrochemical CO 2 fixation to produce multi-carbon (C2 + ) products. However, it is challenging to construct highly stable Cu Ⅰ sites due to the changeability of its valence state and electronic structure in the electroreduction process, and the mechanism is also elusive. Herein, a 2D conductive Cu Ⅱ -MOF film (Cu Ⅱ HHTP@Cu 0 ) is prepared on Cu (1 0 0) foil by in-situ electrochemical assembly. After pre-reduction, the in-situ produced Cu 2 O clusters are confined in the cavities of the film created by the local breaking of the MOF framework to construct a unique Cu Ⅰ -Cu Ⅱ HHTP@Cu 0 catalyst, so that a novel strategy is proposed to stablize highly dispersed Cu Ⅰ sites during electrochemical reduction, thus protecting the Cu 2 O clusters from further reduction and ensuring the coexistence of Cu Ⅰ (1 1 1)-Cu 0 (1 0 0) sites. As such, the current density of CO 2 electrochemical reduction catalyzed by this catalyst exceeds 300 mA cm −2 , the duration overs 50 h, and the C2 selectivity achieves 72.6 %. In-situ spectroscopy and theoretical calculations reveal that the optimized microenvironment of the Cu Ⅰ (1 1 1) sites reduces the energy barrier of *OCCOH coupling, and Cu 0 (1 0 0) sites increase the coverage of *CO, thus boosting the production of C2 products.
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COMPOSITES PART B-ENGINEERING
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2024
·
IF 12.7
Junjie Xu,Wei Sun,Xiang Xiong,Hongbo Zhang
Compared with the traditional C/C composites modified by ultra-high-temperature ceramics (C/C-UHTCs), those modified by metal/medium-entropy ceramics have excellent mechanical properties, thermophysical properties, and long-term ablation resistance. These composites have great potential towards improving the high-temperature resistance and service life of thermal protection systems for spacecraft. In this study, a new type of (Hf 0.5 Zr 0.3 Ti 0.2 )C–W–Cu cermet-modified C/C composites (C/C-(Hf 0.5 Zr 0.3 Ti 0.2 )C–W–Cu) was prepared at 1500 °C. Compared with C/C-UHTCs, the bending strength and fracture toughness of C/C-(Hf 0.5 Zr 0.3 Ti 0.2 )C–W–Cu increased by 70 % and 110 % to 364.25 MPa and 14.64 MPa m 1/2 , respectively. Due to the high thermal conductivity of Cu and W, the thermal conductivity of this new composite was 106 % higher than that of C/C-(Hf 0.5 Zr 0.3 Ti 0.2 )C (44.26 versus 21.53 W/m·K). Under a high heat flow of 4.18 MW/m 2 , this material exhibited very low mass and linear ablation rates (−0.163 mg/s and −0.193 μm/s, respectively). Active and passive protection occur during ablation due to the evaporative cooling of Cu, CuO, and WO 3 as well as a dense outer oxide layer that inhibits oxygen diffusion. The internal oxide layer forms a Hf-Zr-Ti-C-O framework mingled with Ti-rich Ti-Hf-Zr-C-O and an unoxidised W–Cu structure, effectively reducing the osmotic oxygen content. This work provides a new direction for developing thermal protection materials capable of long-term service in ultra-high-temperature environments.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2024
·
IF 8.1
Shuyu Qi,Zilong Zhao,Yingqing Ou,Lu Liu,Yatao Ren,Wenyi Dong,Hongjie Wang,Zigong Ning
In this study, a calcium-rich biochar (CRB)-enhanced dual reaction center (DRC) Fenton-like catalyst, Cu-Al 2 O 3 -CRB, was synthesized for the decomplexation of a typical heavy metal complex pollutant, Ni-ethylenediaminetetraacetic acid (Ni-EDTA), in industrial wastewater. After a 90-minute reaction, the removal rate of Ni(II) could reach 95.2%, regardless of the initial solution pH. Various characterization analyses confirmed the formation of electron-rich Cu regions and electron-deficient Al and C regions in the Cu-Al 2 O 3 -CRB catalyst. The catalytic oxidation experiments, chronoamperometry tests, and reactive oxygen species (ROSs) detection further revealed the roles of biochar and CaCO 3 on the catalytic behavior of Cu-Al 2 O 3 -CRB catalyst. The presence of biochar not only enhanced the loading of Cu species but also introduced additional electron transfer channels, while CaCO 3 further interacted with biochar/Cu species to amplify the electron cloud density surrounding Cu. The improvement of electronic polarization facilitated the activation of H 2 O 2 and the generation of •O 2 – , 1 O 2 and •OH. Contrary to conventional interfacial reactions, the weak adsorption between Cu-Al 2 O 3 -CRB and Ni-EDTA due to the electrostatic repulsion, led to the decomplexation reactions primarily occurring in the solution phase, rather than on the catalyst surface. This study sheds light on the potential of Cu-Al 2 O 3 -CRB as a DRC catalyst and emphasizes the significance of contaminant properties when using this kind of catalysts.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
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IF 7.7
Siying Pei,Wenhui Ji,Ya Yang,Shuo Yang,Tianwei Liu,Jiangxuan Dai,Chan Fu,Jiayi Wu,Yu Yin,Qiong Wu,Bing Zheng,Lin Li
Wearable sweat sensors have made significant progress in the field of human health monitoring, with the ability to achieve continuous collection, monitoring, and transmission of sweat. Current wearable sweat sensors are predominantly enzyme-based, which presents limitations such as high cost, inadequate long-term stability, and vulnerability to environmental conditions. In this study, we present a printed enzyme-free wearable potentiometric sensor for monitoring sweat lactate. Specific and highly sensitive electrocatalysis of sweat lactate was achieved by constructing composite nanomimetic enzyme materials, which involved modifying a screen-printed working electrode with multi-walled carbon nanotubes (MWCNTs) and copper metal-organic framework materials ( Cu-HHTP ). The conversion of divalent copper ions (Cu 2+ ) in Cu-HHTP to monovalent copper ions (Cu + ) in the presence of sodium chloride (NaCl) led to the rapid electrocatalytic oxidation of lactate into pyruvate. The incorporation of MWCNTs enhanced the conductivity and electron transport efficiency of Cu-HHTP , thereby improving the sensitivity. The sensor exhibited an exceptionally low detection limit (1.09 μM), remarkably high sensitivity in the ranges of 1–10 mM (118.55 mV/decade) and 10–50 mM (47.98 mV/decade), as well as outstanding selectivity, reproducibility, and stability. Finally, the developed wearable potentiometric sensor continuously and dynamically monitored changes in sweat lactate levels during physical exercise. This study presents a novel enzyme-free method for the detection of sweat lactate, which holds promise as a valuable support for personalized health diagnosis.
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高影响因子
LANGMUIR
·
2024
·
IF 3.7
Wei-Guang Xiong,Chang-Yong Li,Ranjith Kumar Kankala,Ai-Zheng Chen,Shi-Bin Wang
There has been a growing emphasis on facile preparation of binary heterogeneous composite materials. Leveraging the eco-friendly efficiency of supercritical CO2 technology, we achieved precise control over the influencing factors of mass transfer, enabling the accurate modulation of the resulting product morphology and properties. In the current study, CuxO/ZrOy composite materials were prepared using this technology and calcined to obtain electrode materials for the detection of cysteine (Cys). Essential comprehensive characterization techniques were employed to elucidate the heterojunction. The resulting electrode demonstrated a linear response to Cys within a concentration range of 0.5 nM to 1 μM, featuring a high sensitivity of 1035 μA·cm–2·μM–1 and a low detection limit of 97.3 nM. Thus, establishing a novel avenue for nonenzyme-based electrochemical sensors tailored for biologically active Cys detection through the implementation of a heterogeneous structure.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yan Sun,Zhicheng Wang,Xiaohui Wu,Wen-Da Oh,Mingjie Huang,Tao Zhou
Molecular oxygen (O 2 ), a reactive oxygen species precursor, has great potential for water purification, but its spin-forbidden resistance hinders its direct involvement in the redox reactions of organic compounds under mild ambient conditions. In this study, a facile strategy was employed to synthesize sulfide-modified Fe-Cu bimetallic catalysts (FeCu-S) to enhance the activation efficiency of O 2 . The results showed that the FeCu-S material exhibited a tenfold increase in O 2 activation compared to that of the control group, primarily attributed to the increased specific surface area of the catalysts with an optimized Fe/Cu site distribution and promoted generation of Cu-containing crystalline phases (e. g., CuFeS 2 and CuO) to significantly enhanced the oxygen storage capacity. Moreover, such Cu-containing crystalline phases strengthened surface electron transfer, thus remarkably promoting the selective generation of singlet oxygen ( 1 O 2 ) for sulfamethoxazole (SMX) degradation. The FeCu-S/O 2 system maintained high activity after the fifth recycle with stable reaction pH and negligible leaching of Cu and Fe ions, also exhibited great performance in the advanced treatment of the real biological effluent and ultrafiltration effluent from landfill leachate, confirming its great potential for practical water purification applications.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·
IF 2.7
Jianying Huang,Ling Xia,Xiaohua Xiao,Gongke Li
A copper/polydimethylsiloxane (Cu/PDMS) microfluidic chip was designed to synthesize SERS substrates through a displacement reaction between silver and copper in microfluidic channels to form a microfluidic SERS nanoparticles (AgNPs)/Cu chip. This SERS substrate fabrication method doesn’t require complex manufacturing processes or expensive equipment, and it can be achieved at room temperature in just 4 min. Meanwhile, the AgNPs grown on copper foil could offer ample hot spots, effectively boosting Raman signals of target molecules, and the enhancement factor (EF) was 5.12 × 107 for methylene blue, 4.08 × 107 for crystal violet, and 4.81 × 105 for sulfadiazine. The chip could be reused at least 9 times with consistent signal performance. Combined with the concentration gradient microchannel network design, we could establish the standard curve for samples through one injection on a single chip. A rapid SERS method for detecting sulfadiazine in aquatic products was developed. The linear range for sulfadiazine analysis was 75.0–1000 μg L−1 with a limit of detection of 28.4 μg L−1. Sulfadiazine was found in aquatic products (43.8 μg kg−1), with recovery rates ranging from 82.7% to 110%. The result was validated using high-performance liquid chromatography, showing a relative error of 9.3%. The entire detection procedure could be completed in just 20 min to detect sulfadiazine. These results demonstrate that the developed SERS method is fast, accurate, and reliable for analysis of sulfadiazine in aquatic products.
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