JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2023
·Vol. 103
·期 15
·7631-7642
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IF 4.1
Dongshuang Liang,Haosong Wen,Yaxuan Zhou,Taohong Wang,Guangqun Jia,Zongyan Cui,Adan Li
Background Honey aroma is one of its most important properties and it depends on the qualitative and quantitative composition of the volatile compounds. The volatile profile of honey could reveal its botanical origin to avoid a false characterization. Thus, it is of great significance to honey authentication. This study developed and validated a headspace solid-phase microextraction and gas chromatography–mass spectrometry (HS-SPME-GC–MS) method for simultaneous qualitative and quantitative analyses of 34 volatile components in honey. The developed method was applied to 86 honey samples from six different botanical origins, including linden honey, rape honey, jujube honey, vitex honey, lavender honey and acacia honey. Results The volatile fingerprints and quantitative results were simultaneously obtained by using the full scan and selected ion monitoring (SCAN+SIM) MS scanning mode. The limits of quantification (LOQs) and limits of detection (LODs) of 34 volatile compounds were in the ranges of 1–10 ng/g and 0.3–3 ng/g, respectively. And the spiked recoveries ranged between 70.6% and 126.2%, with the relative standard deviations (RSDs) not higher than 45.4%. A total of 98 volatile compounds were found with relative contents determined, and the 34 volatile compounds were determined with absolute concentrations. Based on the volatile fingerprints and the contents of volatile compounds, honey samples from six botanical origins were well classified by principal component analysis and orthogonal partial least-squares discrimination analysis. Conclusions The HS-SPME-GC–MS method was successfully applied to achieve the volatile fingerprints of six types of honey and to quantitatively analyze 34 volatile compounds with satisfying sensitivity and accuracy. Chemometrics analysis showed significant correlations between honey types and volatiles. These results reveal the characteristics of volatile compounds in six types of unifloral honey and provide some supports for honey authentication. © 2023 Society of Chemical Industry.
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高影响因子
Bioactive Materials
·
2023
·
IF 18.9
Chunchun Xue,Jia Tian,Zepeng Cui,Yang Liu,Dawei Sun,Mengting Xiong,Nanxing Yi,Kaiqiang Wang,Xiaofeng Li,Yongjun Wang,Hao Xu,Weian Zhang,Qianqian Liang
Osteoarthritis (OA) is a common chronic inflammatory disorder. Effective remodeling of inflammatory microenvironment in the joint is a promising strategy to prevent OA. However, current drugs remain unsatisfactory due to a lack of targeted and effective ways for relieving inflammatory conditions in OA joints. Bortezomib (BTZ), a proteasome inhibitor, could effectively inhibit proinflammatory cytokines but with poor accumulation in the inflammatory tissues. To overcome the shortcomings of BTZ delivery and to improve the efficacy of OA therapy, herein, we designed a novel nanomedicine (denoted as BTZ@PTK) by the co-assembly of BTZ and an amphiphilic copolymer (denoted as PTK) with ROS-cleaved thioketal (TK) linkages. The TK units in BTZ@PTK are first cleaved by the excessive ROS at OA sites, and then triggered the controlled release of BTZ, resulting in the accurate delivery and the inflammatory microenvironment remodeling. Accordingly, BTZ@PTK suppressed ROS generation and proinflammatory cytokines while promoting M1 macrophage apoptosis in lipopolysaccharide (LPS)-activated RAW264.7 macrophages or LPS/IFN-γ-treated primary macrophages, which leads to a better effect than BTZ. In OA mice, BTZ@PTK passively accumulates into inflamed joints to attenuate pain sensitivity and gait abnormality. Importantly, BTZ@PTK treatment successfully ameliorates synovitis with the reduction of synovial hyperplasia and synovitis scores by suppressing M1 macrophage polarization and promoting M1 macrophage apoptosis in the synovium, thereby delaying cartilage damage. Collectively, BTZ@PTK can effectively modulate inflammatory microenvironment for OA recession by activating M1 macrophage apoptosis and inhibiting M1macrophage-mediated inflammatory response.
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DESALINATION
·
2023
·
IF 9.9
Xinxin Wei,Kaiming Fan,Peng Cheng,Yanling Liu,Shengji Xia
Simultaneous enhancement in the removal of organic micropollutants (OMPs) and water permeance is highly desirable for nanofiltration (NF)-based drinking water treatment . This study employed two cyclodextrin derivatives with different functional groups, i.e., (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD) and sulfobutylether-β-cyclodextrin (SBECD), as aqueous additives for polyamide (PA) membrane fabrication. Characterization results revealed that HP-β-CD tended to enrich piperazine (PIP) monomers through hydrogen bonding, resulting in a thin but dense PA layer. Conversely, SBECD moderated PIP diffusion through electrostatic forces and steric hindrance , participated more in the interfacial polymerization reaction and yielded an active layer with a coexistence of polyester and polyamide. Both the CD derivative modified membranes exhibited improved surface hydrophilicity , enhanced roughness, and reduced active layer thickness, leading to over 60 % water permeance improvement without the obvious variation of pore size. This enabled the optimal membranes, especially the HP-β-CD incorporated one, highly advantageous in overcoming the trade-off effect between water permeance and OMP rejection. Meanwhile, the strongly negatively charged SBECD modified membrane showed reduced rejections for divalent cations, making it more resistant to scaling. This study offers an efficient approach to tailoring membrane water/solute selectivity and solute/solute selectivity, which has great potential in NF membrane development for drinking water treatment.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Deyi Zhou,Yuelin Xin,Baoguang Wu,Xiaofen Jiang,Xinling Wu,Pengfei Hou,Jiangtao Qi,Jinsong Zhang
In this research, zein was modified with curcumin to obtain covalent and non-covalent complexes. They were further covered with polysaccharides (gum arabic or gum karaya) possessing different surface charge amounts to obtain ternary nanoparticles for preparing novel antioxidant Pickering emulsions. The addition of curcumin to the zein-polysaccharide system significantly retarded the UV degradation of the encapsulated β-carotene (maximum retention ∼ 97%) and effectively inhibited the lipid oxidation of the emulsions. In vitro gastrointestinal digestion assays showed that gum karaya significantly delayed the release of free fatty acids, thereby improving the bioaccessibility of β-carotene (the highest bioavailability ∼ 38%). By comparing the performance of the complex particles, the weakly charged polysaccharides were superior to the highly charged ones, while zein-curcumin covalent binding was superior to non-covalent binding in the above experiments. This study provides innovative perspectives on the use of novel Pickering emulsions to provide ideal protection and bioavailability of lipophilic nutraceuticals.
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CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Lizhu Zeng,Chenjia Lin,Peiqing Liu,Duanping Sun,Jing Lu
Cardiac troponin I (cTnI), a principal biomarker of acute myocardial infarction, possesses superior cardiac specificity and can provide a long detection window for cardiac injury. Although the potential of cTnI as a cardiac biomarker is promising, sensitive and rapid detection of cTnI remains challenging. In this work, a novel colorimetric biosensor was developed for cTnI detection based on multifunctional hybrid nanoprobes coupled with triple-aptamer-modified tetrahedral DNA nanostructures (TAPT-TDNs). First, we evaluated the affinity of four TAPT-TDNs with three vertices modified by two kinds of aptamers in different proportions. The four TAPT-TDNs exhibited much better affinity than the single-stranded aptamer for cTnI, which was attributed to the fact that TAPT-TDNs could improve the capture efficiency of cTnI with excellent interface density and constant support. Second, the selected TAPT-TDNs with highest affinity were immobilized on Fe 3 O 4 -Au magnetic nanoparticles as capture probes (named Fe 3 O 4 -Au-TAPT-TDNs). TAPT-TDNs and HRP were both labeled on NH 2 -MIL-101(Fe) to form signaling probes (named MIL-101(Fe)-HRP-TAPT-TDNs). Then, with the help of capture probes and signaling probes, the target protein was recognized and captured to form capture probe-cTnI-signaling probe sandwich-like structures, causing a decrease in signaling probes in the supernatant with magnetic separation and eventually leading to a visual color change. This colorimetric aptasensor displays excellent analytical performance toward cTnI with a detection limit of 27 pg·mL −1 . The anisotropic aptamer-mediated colorimetric sensor may serve as an alternative to conventional immunoassays for quick and accurate detection of cTnI in clinical samples.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
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IF 8.6
Wenyuan Lu,Lanyue Qi,Dachang Dong,Xianbao Shen,Lijie Xu,Ying Zhang,Xiang Mei,Weichuan Qiao,Xuewen Guo,Yuwei Pan
In this study, g-C 3 N 4 catalysts were prepared from six different precursors including urea, melamine and its hydrochloride, dicyandiamide, thiourea, trithiocyanuric acid to compare their photocatalytic performance and the activation of three commonly seen peroxides, i.e., H 2 O 2 , peroxymonosulfate (PMS), and peroxydisulfate (PDS) under visible light irradiation. The structural and photoelectric properties were analyzed systematically. Results showed that the g-C 3 N 4 synthesized from trithiocyanuric acid (TCA-CN) showed the largest SSA (56.59 m 2 /g), the narrowest band gap (2.621 eV), and lowest carriers’ recombination rate, which correspondingly exhibited the best photocatalytic performance for the degradation of bisphenol A (BPA). Moreover, the g-C 3 N 4 synthesized by urea (U-CN) demonstrating the strongest photoelectron density, widest band gap (2.790 eV) and the most positive VB potential showed the highest activating efficiencies of all three oxidants. In general, persulfates were more competent to enhance the degradation of BPA than H 2 O 2 , and PMS showed the highest activity. The dominant reactive oxidation species (ROS) depended more on the type of peroxides than on the catalyst precursors. The effective activation of PDS by U-CN was primarily resulting from the photoelectrons to produce extra •OH to enhance compounds degradation, and high yield of 1 O 2 could be achieved by PMS activation through h + oxidation, leading to the most significant enhancement of BPA removal. The N–C 3 and NH x of U-CN were likely the sites for PMS activation. Moreover, the U-CN/LED/PMS process showed the strong adaptability to the complex water environment. This work may provide new insights into selecting highly efficient g-C 3 N 4 -based advanced oxidation processes for water remediation.
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Biomaterials Advances
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2023
·
IF 0
Shilong Wu,Qin Zhang,Qiyao Zhao,Yu Jiang,Xiaomeng Qu,Yifan Zhou,Tingting Zhao,Feng Cang,Yanyan Li
Nanotechnology has revolutionized the field of therapeutics by introducing a plethora of nanomaterials capable of enhancing traditional drug efficacy or paving the way for innovative treatment methods. Within this domain, we propose a novel Cobalt-doped hollow polydopamine nanosphere system. This system, incorporating Doxorubicin loading and hyaluronic acid (HA) surface coating (CoHPDA@DOX-HA), is designed for combined tumor therapy. The overarching aim is to diminish the administration dosage, mitigate the cytotoxic side effects of chemotherapy drugs, augment chemosensitivity within neoplastic tissues, and attain superior results in tumor treatment via combined therapeutic strategies. The targeted molecule, hyaluronic acid (HA), amplifies the biocompatibility of CoHPDA@DOX-HA throughout circulation and fosters endocytosis of the nanoparticle system within cancer cells. This nanosphere system possesses pH sensitivity properties, allowing for a meticulous drug release within the acidic microenvironment of tumor cells. Concurrently, Polydopamine (PDA) facilitates proficient photothermal therapy upon exposure to 808 nm laser irradiation. This process further amplifies the Glutathione (GSH) depletion, and when coupled with the oxygen production capabilities of the Cobalt-doped hollow PDA, significantly enhances the chemo-photothermal therapeutic efficiency. Findings from the treatment of tumor-bearing mice substantiate that even at dosages equivalent to a singular DOX administration, the CoHPDA@DOX-HA can provide efficacious synergistic therapy. Therefore, it is anticipated that multifunctional nanomaterials with Photoacoustic Tomography (PAT) imaging capabilities, targeted delivery, and a controlled collaborative therapeutic framework may serve as promising alternatives for accurate diagnostics and efficacious treatment strategies.
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Science China-Technological Sciences
·
2023
·Vol. 66
·期 10
·2797-2807
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IF 4.6
Jiang Miao,Xiao Chao,He XuSheng,Du HuiChao,Wang YanYan,Ding Xin,Zhang Xian,Li XiaoFei,Zheng Kang,Liu XiangLan,Chen Lin,Tian XingYou
In hot environments, the human body shows an efficient capability to maintain a stable temperature by benefiting from sweating behavior. Inspired by this skin perspiration strategy, in this study, we demonstrated an innovative polyimide foam (PIF)-based mimetic skin with excellent cooling capability by integrating a silver coating and reusable hydrogel for the first time. Because of the hybrid thermal dissipating system, the successive silver coating quickly transferred heat to the inside of the polyacrylamide hydrogel. Meanwhile, the hydrogel absorbed a large amount of heat due to its large enthalpy and effectively dissipated heat to the environment through the evaporation of moisture, similar to the sweating of skin. Thus, the temperature of the skin-like film was reduced by 25.4°C compared with pure PIF under a high-power laser heating source. Identical and remarkable cooling effects were also obtained in mobile phone and battery applications, far better than commercially available thermally conductive polyimide (PI). This outstanding performance paves a new way for the thermal management application of PI in wearable electronics, microprocessors, and flexible electronics.
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Nanoscale
·
2023
·
IF 6.7
Yaqing Liu,Zongzhang Wang,Yiru Wang,Yushuo Feng,Mengjiao Xu,Xiaoqian Ma,Qianqian Shi,Huaping Deng,Fangfang Ren,Yong Chen,Hongmin Chen
Rheumatoid arthritis (RA) is a common autoimmune disease, and the inflammatory response during its development can lead to joint cartilage and bone damage up to disability. Dexamethasone (DEX) can effectively alleviate the inflammatory response in RA, but the severe adverse effects that occur after long-term administration limit its clinical development. Herein, we propose a Ca-DEX biomineralization-inducing nut (CaCO3-DEX) with controlled-release properties for mitigating the toxic side effects of DEX in RA treatment, especially the damage to cartilage and bone. CaCO3-DEX releases the drug and Ca2+ preferentially in an inflammatory environment. Both in vitro and in vivo studies demonstrate that CaCO3-DEX significantly reduces the secretion of pro-inflammatory factors and inhibits ROS production in vitro, as well as demonstrates superior pro-biomineralization and osteogenic differentiation potential. In the collagen-induced rheumatoid arthritis model (CIA model), CaCO3-DEX significantly reduces the clinical score of arthritis in mice, and imaging results show a noticeable relief of edema and bone erosion in CIA model mice treated with CaCO3-DEX, while inflammatory factors at the injury areas are significantly reduced, which provides favorable protection to cartilage and bone.
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高影响因子
MOLECULAR MEDICINE
·
2023
·Vol. 29
·期 1
·1-19
·
IF 5.7
Han Zhiyang,Hu Haidi,Yin MingZhu,Lin Yu,Yan Yan,Han Peng,Liu Bing,Jing Bao
Background Macrophage-like transformation of vascular smooth muscle cells (VSMCs) is a risk factor of atherosclerosis (AS) progression. Transcription factor homeobox A1 (HOXA1) plays functional roles in differentiation and development. This study aims to explore the role of HOXA1 in VSMC transformation, thereby providing evidence for the potential mechanism of AS pathogenesis.Methods High fat diet (HFD)-fed apolipoprotein E knockout (ApoE−/−) mice were applied as an in vivo model to imitate AS, while 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine (POV-PC)-treated VSMCs were applied as an in vitro model. Recombinant adeno-associated-virus-1 (AAV-1) vectors that express short-hairpin RNAs targeting HOXA1, herein referred as AAV1-shHOXA1, were generated for the loss-of-function experiments throughout the study.Results In the aortic root of AS mice, lipid deposition was severer and HOXA1 expression was higher than the wide-type mice fed with normal diet or HFD. Silencing of HOXA1 inhibited the AS-induced weight gain, inflammatory response, serum and liver lipid metabolism disorder and atherosclerotic plaque formation. Besides, lesions from AS mice with HOXA1 knockdown showed less trans-differentiation of VSMCs to macrophage-like cells, along with a suppression of krüppel-like factor 4 (KLF4) and nuclear factor (NF)-κB RelA (p65) expression. In vitro experiments consistently confirmed that HOXA1 knockdown suppressed lipid accumulation, VSMC-to-macrophage phenotypic switch and inflammation in POV-PC-treated VSMCs. Mechanism investigations further illustrated that HOXA1 transcriptionally activated RelA and KLF4 to participate in the pathological manifestations of VSMCs.Conclusion sHOXA1 participates in AS progression by regulating VSMCs plasticity via regulation of NF-κB p65 and KLF4. HOXA1 has the potential to be a biomarker or therapeutic target for AS.
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Aging and Disease
·
2023
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IF 7.4
Guoxiang Liu,Xiaoxia Li,Fanghao Yang,Jingyu Qi,Lipeng Shang,Huhu Zhang,Shuang Li,Fenghua Xu,Lingne Li,Huaxin Yu,Yang Li,Xiaolei Dong,Qinghang Song,Feng Zhu,Guang Chen,Can Cao,Liangqian Jiang,Junzhe Su,Lina Yang,Xiaohui Xu,Zhe Zhang,Robert Chunhua Zhao,Bing Li
The senescence of mesenchymal stem cells (MSCs) impairs their regenerative capacity to maintain tissue homeostasis. Numerous studies are focusing on the interventions and mechanisms to attenuate the senescence of MSCs. C-phycocyanin (C-PC) is reported to have multiple functions such as antitumor, antioxidation, anti-inflammation and anti-aging roles, but there is little research about the effects of C-PC on the senescence of MSCs. Here we investigated the roles and mechanism of C-PC on MSCs senescence. In vitro results showed that C-PC could reduce senescence, enhance proliferation, promote the adipogenic and osteogenic differentiation in senescent MSCs induced by oxidative stress. In vivo D-Galactose (D-Gal) induced rats aging models showed C-PC also increased the viability and differentiation of intrinsic senescent bone marrow derived MSCs (BMSCs). Furthermore, C-PC also decreased the levels of oxidative stress markers ROS or MDA, elevated the SOD activity, and increased the anti-inflammatory factors. Proteomic chip analysis showed that C-PC interacted with ZDHHC5, and their interaction was verified by pull down assay. Overexpression of ZDHHC5 aggravated the senescence of MSCs and greatly lessened the beneficial effects of C-PC on senescence. In addition, we found ZDHHC5 regulated autophagy by altering LC3, Beclin1 and PI3K/AKT/mTOR pathway. In summary, our data indicated that C-PC ameliorates the senescence of MSCs through zinc finger Asp-His-His-Cys (DHHC) domain-containing protein 5 (ZDHHC5) mediated autophagy via PI3K/AKT/mTOR pathway. The present study uncovered the key role of autophagy in MSCs senescence and PI3K/AKT/mTOR pathway may be a potential target for anti-senescence studies of MSCs.
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PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2023
·
IF 2.9
Jifeng Wang,Shanshan Tang,Shuang Guo,Dongyu Gu,Yi Wang,Jing Tian,Yi Yang
Agaricus bisporus is one of the most widely cultivated edible mushrooms in the world. The chemical components of A. bisporus have a wide range of biological activities. In order to deeply understand the antioxidant properties of A. bisporus, this study conducted an investigation on the components of A. bisporus fermentation. Through the single factor experiment and response surface optimization, it was found that when the C/N ratio was 45:1, the inoculum concentration was 10%, and the fermentation time was 7 d, the n-butanol extract of the fermentation product had the strongest scavenging capacity for free radical generated through 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid (ABTS·+). The concentration for 50% of the maximal effect (EC50) was 0.33 ± 0.01 mg/mL. Moreover, in order to identify the two main components, the elution–extrusion counter-current chromatography (EECCC) was employed for separation, where 5,5'-oxy-dimethyl-bis(2-furfuraldehyde) and 5-(butoxymethyl) furfural were obtained. The antioxidant activity of 5,5'-oxy-dimethyl-bis(2-furfuraldehyde) (EC50 = 0.26 ± 0.01 mg/mL) was superior to that of 5-butylmethyl furfural (EC50 = 1.52 ± 0.02 mg/mL), indicating that 5,5'-oxy-dimethyl-bis(2-furfuraldehyde) was the main antioxidant in the fermentation products. The thermodynamic parameters and frontier molecular orbitals of 5,5'-oxy-dimethyl-bis (2-furanaldehyde) was evaluated by density functional theory (DFT). The result indicated 5,5'-oxy-dimethyl-bis(2-furanaldehyde) scavenged free radicals in polar media through single electron transfer followed by proton transfer (SET-PT).
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危险品化学品经营许可证（带存储）