JOURNAL OF ETHNOPHARMACOLOGY
·
2024
·
IF 4.8
Mengze Ding,Xiaohan Wei,Changshun Liu,Xiaomei Tan
Ethnopharmacological relevance Allergic rhinitis (AR) is a prevalent nasal inflammatory disorder, and pyroptosis plays a crucial role in aggravating AR. Current medications for AR treatment still have deficiencies, and finding new agents is of great interest. Mahuang Fuzi Xixin decoction (MFXD), an ancient Chinese medicine, is now commonly used to treat AR, which has anti-inflammatory and immunomodulatory effects, but its underlying mechanism is unknown. Aim of this study This study aims to evaluate the effects of MFXD on AR and explore its potential mechanisms in view of the regulatory effect on pyroptosis. Methods MFXD, Mahuang, Fuzi, and Xixin water extracts were analyzed using ultra high performance liquid chromatography-Orbitrap-high-resolution accurate mass spectrometry. In in vivo study, the effects of MFXD on AR treatment were evaluated in an ovalbumin-induced mouse model. Mice were administered saline (control and model groups), MFXD (1.375, 2.75 g/kg), and dexamethasone (2.5 mg/kg) for 13 days. AR symptoms were evaluated by blinded observers. Immunoglobulin E (IgE) and histamine levels were measured using enzyme-linked immunosorbent assays. Expression of pyroptosis-related proteins (NLRP3, ASC, Caspase-1 p10/p20, GSDMD-N and IL-1β) in AR mouse nasal mucosa were estimated by immunohistochemistry. In in vivtro study, the effects of MFXD on pyroptosis were assessed in human nasal epithelial cells (HNEpCs) stimulated with lipopolysaccharide (LPS) and adenosine triphosphate (ATP), and incubated with MFXD (12.5, 25, and 50 μg/mL). Pyroptosis-related protein expression was measured by western blotting. Results Thirty-three compounds in MFXD were identified, including ephedrine, pseudoephedrine, higenamine, aconine, aconitine, benzoylmesaconitine, benzoylhypaconine and hypaconitine. In the in vivo study, oral taken of MFXD/dexamethasone significantly ameliorated AR symptoms, reduced swelling of the nasal mucosa, and decreased the levels of IgE and histamine in AR mice serum. MFXD/dexamethasone attenuated histopathological changes and reduced the expression of pyroptosis-related proteins in nasal mucosa, indicating the inhibitory effect on nasal epithelial pyroptosis. In the in vitro study, MFXD (50 μg/mL) significantly alleviated cytotoxicity, protected cells from swelling and rupture, and downregulated the expression of pyroptosis-related proteins in LPS/ATP-induced HNEpCs. Conclusion MFXD suppressed nasal epithelial pyroptosis by inhibiting the NLRP3/Caspase-1/GSDMD-N signaling pathway, which alleviates AR. Our results offer valuable insights into potential AR therapies and provide evidence for the clinical utilization of MFXD to treat AR.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Simiao Liu,Meixi Liu,Yuzhuo Wang,Xin Liu,Fazheng Ren,Hao Zhang
This study investigated the product characteristics and prebiotic properties of lactose-N-neotetraose (LNnT) after the Maillard reaction with α-lactalbumin (α-LA). LNnT and α-LA were reacted in a simulated system at 60 °C and 79 % relative humidity for 96 h to create Maillard reaction products (MRPs). During the reaction, the absorbances at 294 nm and 420 nm and the fluorescence intensity of α-LA-LNnT increased, whereas the free amino groups and free sugar content decreased. Matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) analysis revealed that each α-LA was attached with eight LNnT molecules. After the reaction, the far-ultraviolet circular dichroism (CD) and fluorescence spectra showed that the random coil content of α-LA-LNnT significantly increased ( p < 0.05), with the side chains of α-LA's tertiary structure affected. In terms of prebiotic properties, the bacterial density of Bifidobacterium decreased from 0.63 to 0.36, and the short-chain fatty acids production decreased from 12.60 mM to 5.38 mM using the in vitro fermentation medium. In summary, various MRPs were formed by the reaction between LNnT and α-LA at different heating stages, and the Maillard reaction reduced the prebiotic properties of LNnT. The findings provide insights into the impact of the Maillard reaction on the prebiotic properties of human milk oligosaccharides and direct our attention to the interactions of nutrients in infant formula.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Science Advances
·
2025
·Vol. 11
·期 9
·
IF 12.5
Mengxue Huang,Xuya Zhu,Wenwen Shi,Qianqian Qin,Jie Yang,Shanshan Liu,Lifang Chen,Ruimin Ding,Lin Gan,Xi Yin
Nitrogen-coordinated metal sites (MNx) in metal- and nitrogen-codoped carbon (M-N-C) catalysts offer promising electrocatalytic activity, but selective synthetic design of MNx sites with specific coordination environments remains challenging. Here, we manipulate the formation statistics of MNx sites by using sacrifice alkali metals (AM = Li, Na, and K) to form metal vacancy-Nx carbon (AM-MVNx-C) templates, which are used to direct the solution-phase formation of CoN4 sites in Co-N-C catalysts. We build a probability weight function based on the embedding energy of M in MNx sites as the descriptor for MNx formation statistics, and we predict that the alkali metals are prone to induce the formation of MVN4 sites. By coordinating Co2+ ions with AM-MVNx-C templates, we synthesize Co-N-C with CoN4 sites, demonstrating remarkable oxygen reduction activity in anion exchange membrane fuel cells. These results highlight the statistical thermodynamics of MNx formation and open up the possibility for the rational design of complex M-N-C electrocatalysts with well-defined MNx sites.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Zhejiang University-SCIENCE B
·
2024
·Vol. 25
·期 1
·51-64
·
IF 4.7
Xu Mingming,Liu Zhaoliang,Hu Wenhua,Han Ying,Wu Zhen,Chen Sufeng,Xia Peng,Du Jing,Zhang Xumin,Hao Piliang,Xia Jun,Yang Shuang
Pancreatic cancer is among the most malignant cancers, and thus early intervention is the key to better survival outcomes. However, no methods have been derived that can reliably identify early precursors of development into malignancy. Therefore, it is urgent to discover early molecular changes during pancreatic tumorigenesis. As aberrant glycosylation is closely associated with cancer progression, numerous efforts have been made to mine glycosylation changes as biomarkers for diagnosis; however, detailed glycoproteomic information, especially site-specific N -glycosylation changes in pancreatic cancer with and without drug treatment, needs to be further explored. Herein, we used comprehensive solid-phase chemoenzymatic glycoproteomics to analyze glycans, glycosites, and intact glycopeptides in pancreatic cancer cells and patient sera. The profiling of N -glycans in cancer cells revealed an increase in the secreted glycoproteins from the primary tumor of MIA PaCa-2 cells, whereas human sera, which contain many secreted glycoproteins, had significant changes of glycans at their specific glycosites. These results indicated the potential role for tumor-specific glycosylation as disease biomarkers. We also found that AMG-510, a small molecule inhibitor against Kirsten rat sarcoma viral oncogene homolog ( KRAS ) G12C mutation, profoundly reduced the glycosylation level in MIA PaCa-2 cells, suggesting that KRAS plays a role in the cellular glycosylation process, and thus glycosylation inhibition contributes to the anti-tumor effect of AMG-510.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Qingsong Luo,Pengfei Zheng,Lina Han,Yidan Fu,Xiaoyuan Zeng,Jing Feng,Yudong Sui,Peng Dong,Yingjie Zhang
Constructing composite structures is an effective strategy for tailoring the electronic configuration and balances the adsorption/desorption capability of oxygen-containing intermediates for obtaining high-performance bifunctional catalysts. Herein, an in-situ pyrolysis strategy was used to construct Fe 2 P nanoparticles supported on N-doped carbon (Fe 2 P/NC) catalyst. The DFT indicated that the catalytic activity of the as-prepared catalyst is significantly increased by the strong electronic interaction between the Fe 2 P nanoparticles and the nitrogen-doped carbon, which endows the catalyst with fast electron transfer capability and optimizes the free energy between the catalyst and the adsorbed intermediate, as well as the d-band center of the material. The as-synthesized Fe 2 P/NC has a low potential gap ΔE (ΔE = E j=10 – E 1/2 ) of ca. 0.75 V, a specific capacity as high as 833 mAh·g Zn − 1 and cycling stability for 1320 cycles at the current density of 10 mA·cm −2 . Such a synthesis strategy provides an effective route to realizing applications for portable electronic Zn-air battery-related devices.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xinyi Wang,Jing Wang,Jian Yang,Houcheng Ding,Yongping Dong
Sensitive and selective determination of neurotransmitter dopamine (DA) holds great significance in clinical disease diagnosis. Constructing highly active electrocatalysts at low costs is a favorable strategy for sensitive electrochemical detection of DA. In this work, taking a template-aided method, single-atom iron supported on hollow N-doped carbon matrix (Fe SA/HNC) was successfully synthesized. After the morphology and component of the prepared catalysts were characterized in details, it was used to construct electrochemical sensor. Given the excellent catalytic activity of Fe SA and the porous spherical structure of HNC, under the optimal conditions, the Fe SA/HNC sensor exhibited superior sensing performance to DA in a wide concentration range from 1.0 to 1000 µM with a low detection limit of 0.32 µM, along with good stability, reproducibility, and repeatability. In addition, the Fe SA/HNC sensor was also adopted to the simultaneous detection of three common small biomolecules including DA, uric acid (UA) and ascorbic acid (AA). Lastly, the applicability of the sensor was also well-demonstrated by detecting DA in real samples of human serum, showing satisfactory recoveries.
✓ 已验证
产品关联
DOI
高影响因子
CATALYSIS LETTERS
·
2024
·
IF 2.3
Sun Min,Chu Siyu,Guo Dongfang,Jiao Xinyu,Wang Haiyan,Wang Lingli,Li Zijiong,Jiang Liying
Rising energy demand has forced to look for new ways to convert energy. Graphene quantum dots (GQD) has received extensive attention in many fields due to their low toxicity, abundant active sites, sound water solubility and photoluminescence stability. Here, a fluorine-nitrogen co-doped GQD (C-GQD) is synthesized for efficient oxygen reduction reaction (ORR) catalysts. The incorporation of fluorine and nitrogen atoms changed the electronic structure of the carbon atom, resulting into synergistic enhancement of catalytic performance toward ORR. Electrochemical studies of the ORR showed that the initial potentials of C-GQD were positively shifted by 200 mV and 170 mV compared with those of GQD and N-GQD, respectively, and the half-wave potentials were positively shifted by 200 mV and 180 mV, which were comparable to that of 20% Pt/C. The CV curves underwent only slight fluctuations after 10,000 cycle tests. The obtained results provide important insights into the use of metal-free catalysts with GQDs as carriers as promising electrocatalysts for proton exchange membrane fuel cells with low cost and easy preparation procedures. Graphical Abstract
✓ 已验证
产品关联
DOI
高影响因子
Separations
·
2025
·Vol. 12
·期 2
·42
·
IF 2.7
Huihui Wang,Yuqiong Zhao,He Lian,Qi Wang,Zhihong Shang,Guojie Zhang
The separation of CH4and N2is essential for the effective use of low-concentration coalbed methane (CBM). In this study, a series of nitrogen-doped porous carbons were synthesized using an in situ nitrogen doping method combined with K2CO3activation. The study systematically examined how changes in the physical structure and surface properties of the porous carbons affected their CH4/N2separation performance. The results revealed that in situ nitrogen doping not only effectively adjusts the pore structure and alters the reaction of K2CO3on the carbon matrix, but also introduces nitrogen and oxygen functional groups that significantly enhance the adsorption capabilities of the materials. In particular, sample S3Y6−800 demonstrated the highest methane adsorption capacity of 2.23 mmol/g at 273 K and 1 bar, outperforming most other porous carbons. This exceptional performance is attributed to the introduction of N-5, N-6, C-O, and COOH functional groups, as well as a narrower pore-size distribution (0.5–0.7 nm) and the formation of carbon nanotube structures. The introduction of heteroatoms also provides additional adsorption sites for the porous carbon, thus improving its methane adsorption capacity. Furthermore, dynamic breakthrough experiments confirmed that all samples effectively separated methane and nitrogen. The Toth model accurately described the CH4adsorption behavior on S3Y6−800 at 298 K, suggesting that the adsorption process follows a sub-monolayer coverage mechanism within the microporous regions. This study provides a mild and environmentally friendly preparation method of porous carbons for CH4/N2separation.
✓ 已验证
产品关联
DOI
高影响因子
Materials
·
2024
·Vol. 17
·期 19
·4804
·
IF 3.1
Fan Zhang,Qiwen Zhang,Feng Zhang,Xiaolin Luo,Wei Wang
N-heterocycles with quinoline structures hold significant importance within the chemical and pharmaceutical industries. However, achieving their efficient transformations remains a vital yet challenging endeavor. Herein, a series of W-doped Ga2O3-NC catalysts were synthesized using a Ga-MOF-derived strategy through a simple solvothermal method, with a remarkably high activity and selectivity towards the oxidative dehydrogenation ofN-heterocycles. Furthermore, the MOF-derived W-doped Ga2O3-NC catalysts exhibit remarkable substrate tolerance and recyclability. The outstanding catalytic activity was attributed to the robust synergistic interaction between the W species and the Ga2O3-NC carrier, which facilitates the activation of hydrogen atoms in the C-H and C=N bonds on both the oxygen molecule and the substrate to produce H2O2. Additionally, the solvent effect of methanol can significantly enhance dehydrogenation due to its strong ability to donate and accept protons of hydrogen bonding. The present work provides a new approach to MOF-derived non-precious metal catalysts for achieving the efficient oxidation dehydrogenation ofN-heterocycles.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS RESEARCH BULLETIN
·
2024
·
IF 5.3
S. Wei,Q. Zhu,C. Wang,X.B. Yang,B. Zhou,Z.Q. Duan,Z.X. Xie,Y.M. Hu
A facile and green approach to composites containing nitrogen doped lotus seedpods biocarbon (NALC) and NiCo 2 S 4 (NCS) is reported. The composites present excellent electrocatalytic activity for reduction from I 3 − to I − due to the synergistic effect between the nanocrystalline NCS and the NALC. Dye-sensitized solar cells (DSSCs) assembled with the as-prepared NALC@NCS counter electrodes (CEs) achieve impressive photovoltaic conversion efficiency (PCE) of 8.58%, which is higher than that by the same structured cell with Pt (7.85%) and NCS (7.53%) CEs. Compared with other NCS based CEs, the NALC@NCS CEs show good durability and stability, suggesting that combining nanocrystalline catalyst with biocarbon is beneficial to stimulate the synergistic effect, and is a feasible strategy to optimal CEs for high performance DSSCs. Low cost NALC@NCS composites developed in this work are environmentally friendly, stable and efficient, which are believed to be good candidates for Pt-free CE materials in DSSCs and electrocatalysis applications.
✓ 已验证
产品关联
DOI
高影响因子
Catalysts
·
2025
·Vol. 15
·期 4
·298
·
IF 4
Yue Zhang,Shi Zheng,Jian Xiao,Jiangbo Xi
Graphene film has excellent electrical conductivity and flexibility, with which it can be used as a versatile substrate to load active species to construct free-standing electrochemical sensors. In this work, Pd nanoparticle-decorated N-doped porous graphene film (Pd/NPGF) was prepared by a simple and mild strategy to enhance the electrochemical behavior of graphene film-based free-standing electrodes. The morphological structure and surface component of the Pd/NPGF were characterized by scanning electron microscopy, transmission electron microscopy, Raman spectra and X-ray photoelectron spectroscopy measurements. The results revealed that the Pd/NPGF contained abundant pores and uniformly dispersed Pd nanoparticles, which could bring a favorable electrochemical response. Due to the synergetic effects of abundant pores, uniform Pd nanoparticles and the substitutional doping of the graphene framework with N, the novel free-standing Pd/NPGF electrode provides a high active site exposure, a high specific area and fast electron/mass diffusion during electrochemical reactions. Considering the favorable flexibility and excellent electrical conductivity of Pd/NPGF, we selected hydrogen peroxide, a significant biomarker, as a model to investigate its electrochemical performance in neutral conditions. The electrochemical biosensor based on the Pd/NPGF electrode exhibited enhanced activity relative to the NPGF and porous graphene film (PGF) with different concentrations of H2O2. The Pd/NPGF electrode displayed a high sensitivity (176.7 μA·mM−1·cm−2), a large linear range from 5 μM to 36.3 mM, a low limit of detection (LOD) of 2.3 μM, excellent stability and a short response time, all of which qualify the Pd/NPGF electrode for a promising sensor for H2O2sensing.
✓ 已验证
产品关联
DOI
高影响因子
Molecular Catalysis
·
2024
·
IF 3.9
Hui Cao,Yu Liu,Yanqiu Li,Yingxiong Wang,Yan Qiao
The α-hydroxyketones and 1,2-diols are versatile building blocks for the preparation of many natural and synthetic bioactive compounds. In this study, N-heterocyclic carbon (NHC) promoted coupling of several aldehydes with formaldehyde enabled the synthesis of 1,2-diols in moderate to high yields. The highly selective conversion of several aldehydes and formaldehyde into 1,2-diols and α-hydroxyketones was achieved in a one-pot two-step process via [BHMIm][OAc] as a key intermediate. In the first step, formaldehyde was quantitatively converted to [BHMIm][OAc] through the carbene addition. In the second step, 1,2-diol was produced through the intermediate-promoted aldol reaction. The results showed that benzaldehyde was capable to react with [BHMIm][OAc] efficiently, with a 54.1% yield of 1,2-diol after the 3-hour reaction at 85 °C. Among the tested benzaldehyde derivatives, the 4-nitrobenzaldehyde showed the highest yield of 1,2-diol (73.3%) under the optimized reaction conditions. Incorporation of an electron rich group on benzaldehyde promoted carbene regeneration. With 4-methoxybenzaldehyde as the substrate, the yield of α-hydroxyketone reached a maximum of 30.6%. Additionally, dihydroxyacetone derivatives were detected in the system. In situ NMR technique confirmed that it was generated from the further reaction of the carbene/1,2-diol complex with another aldehyde. Based on the results of NMR spectroscopy and mass spectrometry, a possible reaction pathway was also proposed.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）