INFLAMMATION RESEARCH
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2024
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IF 4.8
Su Pan,Li Zibo,Yan Xiangli,Wang Baoying,Bai Ming,Li Yucheng,Xu Erping
Objective and design Mast cells (MCs), as the fastest immune responders, play a critical role in the progression of neuroinflammation-related diseases, especially in depression. Quercetin (Que) and kaempferol (Kae), as two major diet-derived flavonoids, inhibit MC activation and exhibit significant antidepressant effect due to their anti-inflammatory capacity. The study aimed to explore the mechanisms of inhibitory effect of Que and Kae on MC activation, and whether Que and Kae suppress hippocampal mast cell activation in LPS-induced depressive mice. Subjects and treatment In vitro assays, human mast cells (HMC-1) were pretreated with Que or Kae for 1 h, then stimulated by phorbol 12-myristate 13-acetate (PMA) and 2,5-di-t-butyl-1,4-benzohydroquinone (tBHQ) for 3 h or 12 h. In vivo assays, Que or Kae was administered by oral gavage once daily for 14 days and then lipopolysaccharide (LPS) intraperitoneally injection to induce depressive behaviors. Methods The secretion and expression of TNF-α were determined by ELISA and Western blotting. The nuclear factor of activated T cells (NFAT) transcriptional activity was measured in HMC-1 stably expressing NFAT luciferase reporter gene. Nuclear translocation of NFATc2 was detected by nuclear protein extraction and also was fluorescently detected in HMC-1 stably expressing eGFP-NFATc2. We used Ca 2+ imaging to evaluate changes of store-operated calcium entry (SOCE) in HMC-1 stably expressing fluorescent Ca 2+ indicator jGCamP7s. Molecular docking was used to assess interaction between the Que or Kae and calcium release-activated calcium modulator (ORAI). The hippocampal mast cell accumulation and activation were detected by toluidine blue staining and immunohistochemistry with β-tryptase. Results In vitro assays of HMC-1 activated by PtBHQ (PMA and tBHQ), Que and Kae significantly decreased expression and secretion of TNF-α. Moreover, NFAT transcriptional activity and nuclear translocation of NFATc2 were remarkably inhibited by Que and Kae. In addition, the Ca 2+ influx mediated by SOCE was suppressed by Que, Kae and the YM58483 (ORAI inhibitor), respectively. Importantly, the combination of YM58483 with Que or Kae had no additive effect on the inhibition of SOCE. The molecular docking also showed that Que and Kae both exhibit high binding affinities with ORAI at the same binding site as YM58483. In vivo assays, Que and Kae significantly reversed LPS-induced depression-like behaviors in mice, and inhibited hippocampal mast cell activation in LPS-induced depressive mice. Conclusions Our results indicated that suppression of SOCE/NFATc2 pathway-mediated by ORAI channels may be the mechanism of inhibitory effect of Que and Kae on MC activation, and also suggested Que and Kae may exert the antidepressant effect through suppressing hippocampal mast cell activation.
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ACS Sustainable Chemistry & Engineering
·
2024
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IF 7.1
Rui Huang,Shaohua Luo,Pengyu Li,Qi Sun,Guodong Hao,Jie Feng,Lixiong Qian,Shengxue Yan,Jing Guo
Mn-rich ternary cathodes are highly regarded as a potential option for sodium-ion batteries (SIBs) due to their low cost and high theoretical capacity. Nonetheless, cycling stability was hindered by the occurrence of high-voltage phase transitions. In this work, Na0.67(Ni0.1Mn0.8Fe0.1)1–xMgxO2 (NaNMF-Mgx) cathode materials with high-voltage zero-phase transitions property were successfully synthesized. Amazingly, it was found that there was a valuable strengthening in the occupancy of stabler Nae sites by employing the Mg-doping strategy; and the calculation highlighted excellent structural stability and conductivity of NaNMF-Mg0.04, which has the lowest thermodynamic formation energy and a narrow band gap. The combination of theory and experiment demonstrated the underlying mechanisms of Mg substitution. Especially, Mg doping had the potential to regulate Nae/Naf ratio, and the ratio of NaNMF-Mg0.04 reached the maximum, indicating its most remarkable “rich-Nae” effect. Moreover, ex-situ XRD and ADF-STEM certified that NaNMF-Mg0.04 cathode maintained an intact P2 phase structure during high-voltage charging process. The “rich-Nae” and “zero-phase transitions” effects enabled NaNMF-Mg0.04 cathode to express remarkable initial capacitance (119.5 mAh g–1, 0.1 C), stability (80.0% over 200 cycles), and energy density (356.5 Wh kg–1). This unique mechanism provided fresh insights into revisiting the relationship between structure and performance and might open up a new idea for designing novel Mn-rich ternary cathodes with zero-phase transitions property in the future.
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ACS Applied Polymer Materials
·
2024
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IF 4.4
Shuai Han,Junjian Yu,Fei Wang,Xiangwei Li,Hongzhe Ni,Yuchao Wang,Zhe Wang
Zeolite is a commonly utilized filler in mixed matrix membranes (MMMs) to enhance the effectiveness of gas separation due to its molecular sieving properties with high separation coefficients. However, the compatibility and suitability between the zeolite and the polymer membrane are prerequisites for preparing homogeneous membranes and an essential factor affecting gas separation performance. Herein, we synthesized nucleus-crown-crown structures by surface modification of SOD zeolite and prepared MMMs by doping them into Polymers of Intrinsic Microporosity (PIM-1) for efficient gas separation. FTIR, XRD, SEM, and TGA characterized the fillers and the MMMs. The average particle size of the filler was estimated to be approximately 552.43 nm by SEM images. The surface-modified SOD zeolite facilitates the rapid permeation of specific gas molecules and enhances the dispersion of SOD zeolite in the PIM-1 membrane, thereby improving gas separation performance. Compared with pure PIM-1 membrane, the MMMs with a loading of 7 wt % exhibited CO2 and H2 permeabilities reaching up to 7000.0 and 3600.0 Barrer; their selectivities for CO2/N2 and H2/N2 were measured at 17.7 and 9.29, respectively. MMMs with the best fill ratios exceeded the 2008 Robeson upper limits. The methods for surface modification of SOD zeolites provide a new way for the application of SOD zeolite in the area of gas separation.
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CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2024
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IF 3.7
Bharat Prasad Sharma,Tianzhang Wang,Yufeng Liang,Jinping Xiong,Liangrong Yang,Zheng Li
Simultaneous recovery of Ni and Co from Fe(III) and Al is a critical challenge in hydrometallurgical processes. Recognized solvent extraction systems often struggle with selectivity and effective performance in mixed metal ion environments. Herein, a new synergistic solvent extraction (SSX) system comprised of a novel pyridine analog, N,N -bis(pyridin-2-ylmethyl) dodecan-1-amine (BPMDA), and dinonylnaphthalene sulfonic acid (DNNSA) with tributyl phosphate as phase modifier is introduced. The SSX system demonstrates high extraction performance achieving >90% for Ni and >97% for Co in a single-stage extraction process, with high selectivity. Under optimal conditions, the selectivity sequence is observed as Co 2+ (>97%) > Ni 2+ (>90%) > Mn 2+ (<20%) > Fe 3+ (<10%) > Mg 2+ (<5%) > Al 3+ (<2%) > Ca 2+ (<1%). Spectroscopic analysis evidences the preferential binding of BPMDA with Ni and Co in the presence of DNNSA, concurrently achieving a significant reduction in the co-extraction of Fe(III) and Al. The selective complexation of Ni and Co using the SSX system offers a highly efficient and selective approach for their extraction, with promising potential for applications in recovery-based processes.
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World Journal of Stem Cells
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2025
·Vol. 17
·期 7
·104607
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IF 3.6
Kun Wang,Wei Lin,Jun-Yong Han,Jin-Yan Chen,Rong-Hua Liu,Zhen Yu,Jing-Jun Jin
BACKGROUND Type 1 diabetes (T1D) results from the autoimmune-mediated loss of pancreatic β-cells. Current insulin therapies offer symptomatic relief but fall short of providing a definitive cure. Islet cell transplantation, while promising, faces limitations related to donor scarcity, procedural complexities, and the necessity for long-term immunosuppression. Consequently, there is an urgent need for innovative strategies aimed at β-cell regeneration. Patient-derived induced pluripotent stem cells (iPSCs), obtained from peripheral blood mononuclear cells (PBMCs) of T1D patients, hold great potential as a source of cells for therapeutic purposes. Therefore, the differentiation of T1D-iPSCs into functional pancreatic β-cells is a critical step toward effective β-cell replacement therapy. AIM To assess the potential of patient-derived T1D-β-like cells (differentiated from T1D-iPSCs reprogrammed from T1D-PBMCs) for restoring β-cell function in T1D. METHODS T1D-iPSCs were reprogrammed from T1D-PBMCs using an episomal vector-based approach. Pluripotency was confirmed by flow cytometry (FCM), quantitative real-time polymerase chain reaction, genomic stability analysis, and teratoma formation assays. Differentiation into pancreatic β-cells was optimized using triiodothyronine (T3), vitamin C (Vc), and an adenovirus (M3C) encoding pancreatic duodenal homeobox-1, neurogenin 3 ( Ngn3 ), and MAF bZIP transcription factor A ( MafA ). Following characterization of β-cell features by immunofluorescence, quantitative real-time polymerase chain reaction, and flow cytometry, therapeutic efficacy was assessed through blood glucose monitoring after transplantation under the renal capsule of streptozotocin-induced diabetic mice. RESULTS T1D-iPSCs were successfully generated from T1D-PBMCs. These cells exhibited the hallmark characteristics of pluripotent stem cells, including appropriate morphology, differentiation potential, genomic integrity, and expression of pluripotency-associated genes. Differentiation yielded insulin-positive (insulin + ) pancreatic β-like cells that, at the mRNA level, expressed key β-cell markers such as pancreatic duodenal homeobox-1, Ngn3 , MafA , NeuroD , glucagon-like peptide-1 receptor, Nkx6.1 , glucose transporter 2, and Kir6.2 . Notably, the T3 + Vc group displayed the lowest Ngn3 expression (1.31 ± 0.38 vs 1.96 ± 0.25 vs 2.51 ± 0.24, P < 0.01), while the M3C + T3 + Vc group exhibited the highest MafA expression (0.49 ± 0.11 vs 0.32 ± 0.06 vs 0.29 ± 0.08, P < 0.05). Both in vitro and in vivo assessments confirmed the insulin secretion ability of the generated β-like cells; however, they did not demonstrate appropriate modulation of insulin release in response to variations in extracellular glucose concentrations. CONCLUSION T1D-iPSCs derived from T1D-PBMCs can be differentiated into insulin + β-like cells, albeit with functional immaturity. These cells represent a potential source of seed cells for β-cell replacement therapy in T1D.
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Experimental Hematology & Oncology
·
2025
·Vol. 14
·期 1
·1-17
·
IF 13.5
Ren Xueting,Wu Jinpeng,Li Jing,Zhai Zhen,Li Xiang,Guan Feng,Wang Meng,Ma Xiaobin,Tan Zengqi,Kang Huafeng,Lin Shuai
Background The abundance of PD-L1 on the surface of tumor cells is a critical factor in sensitizing these cells to T cell-mediated immune killing. While abnormal glycosylation of PD-L1 is known to influence its expression and function, the precise regulatory mechanisms remain unclear.Methods This study utilized bioinformatics analysis to explore the role of MGAT3, a key gene involved in the formation of the bisecting GlcNAc structure, in breast cancer (BC). Experimental approaches were employed to increase bisecting GlcNAc levels in BC cells, followed by assessments of PD-L1 expression, CD8+ T cell-mediated cytotoxicity, extracellular vesicle (EV)-associated PD-L1, and PD-L1/PD-1 interaction. Additionally, forskolin, a bisecting GlcNAc agonist, was combined with anti-PD-L1 antibody to evaluate its antitumor effects in vivo.Results MGAT3 was found to be expressed at low levels in BC tissues but positively correlated with CD8+ T cell infiltration. Elevating bisecting GlcNAc levels in BC cells significantly enhanced the cytotoxic efficacy of CD8+ T cells. High bisecting GlcNAc modification promoted PD-L1 degradation via the lysosomal pathway, reducing PD-L1 expression and its binding to PD-1. Furthermore, increased bisecting GlcNAc levels reduced PD-L1 in tumor cell-derived EVs, impairing the EVs’ ability to block CD8+ T cells and indirectly enhancing T cell cytotoxicity. The combined use of forskolin and anti-PD-L1 antibody significantly increased CD8+ T cell abundance and activity, achieving a more effective antitumor response in vivo.Conclusion sThese findings demonstrate that enhancing bisecting GlcNAc modification in BC cells promotes PD-L1 degradation and inhibits its binding to PD-1, thereby boosting CD8+ T cell-mediated cytotoxicity, providing a promising strategy for immune modulation in BC therapy.
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JOURNAL OF MOLECULAR STRUCTURE
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2025
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IF 4.7
Hong-Wei Di,Xing-Qiang Zhao,Xin-Ru Zhang,Jiangping Cao,Ya-Ting Wang,Hua He,Jun-Ling Dong,Chao Fan,Nan Zhang
With the development of industry, the treatment of industrial waste water containing metal ions has become a difficult problem in environmental governance. Polymer fluorescent probe is an effective method for identifying and enriching metal ions in waste water. The ring ‒ opening polymerization of ε ‒ caprolactone was initiated with 1 ‒ pyrene methanol as the initiator and the fluorescent group, and then it was chemically linked with the recognition group dimethylpyridine amine by 2‒chloroacetyl chloride to obtain the polymer fluorescent probe L. The chemical structure of polymer fluorescence probe L was confirmed by 1 H NMR. The recognition performance of polymer fluorescence probe L for metal ions was studied by fluorescence spectra. The results show that the polymer fluorescence probe L has specific recognition ability for metal ion Zn 2+ . By optimizing the optical properties, the selectivity and anti-interference ability of fluorescence probe L to Zn 2+ were improved. The linear recognition range of Zn 2+ is from 2 × 10 –6 mol ∙ L -1 to 9 × 10 –6 mol ∙ L -1 , and the detection limit is 6.38× 10 –7 mol ∙ L -1 . According to the plot curve of job, the complex ratio of Zn 2+ and probe L is 1 : 1. The fluorescence principle of coordination between dimethylpyridine amine (DPA) and zinc ion (Zn 2+ ) mainly involves the inhibition of photo induced electron transfer (PET) mechanism. In summary, probe L can be used to detect Zn 2+ in acetonitrile rapidly, so it has a certain application value in the identification and separation of metal ions in industrial waste water.
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JOURNAL OF MOLECULAR STRUCTURE
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2025
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IF 4.7
Wei Ding,Xiaofeng Yuan,Ming Lu,Yuangang Xu
3-Nitro-1-(trinitromethyl)-1 H -pyrazole-4-carboxylic acid ( 4 ) and its corresponding ammonium ( 5 ) and hydroxylammonium ( 6 ) salts were successfully synthesized from 3-amino-1 H -pyrazole-4-carbonitrile. Their structures were characterized by infrared, nuclear magnetic resonance, elemental analysis, and confirmed by single-crystal X-ray diffraction to be triclinic P 1 ¯ ( 4 ), monoclinic C 2 /c ( 5 ) and Cc ( 6 ) Space group with 2, 8, and 4 molecules per unit cell. ADCH charge analysis revealed an uneven electron density distribution across the ring system of 4 , with notably high charge density around two oxygen atoms of the carboxyl group, which facilitates hydrogen bond formation. NICS aromaticity evaluation further confirmed the strong aromatic nature of the product, with an NICS(1)zz value below -20.0 ppm. Remarkably, the hydroxylammonium salt 6 not only retains outstanding detonation properties (detonation velocity D = 8878 m s - ¹, detonation pressure P = 35.60 GPa) but also demonstrates superior mechanical sensitivity (impact sensitivity IS = 12 J, friction sensitivity FS = 200 N). This achieves an optimal balance between high energy and low sensitivity, meeting the design objectives for high-energy insensitive materials. The findings offer valuable insights and experimental support for the molecular design of novel energetic compounds that integrate high energy performance with robust stability.
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CHEMICAL ENGINEERING JOURNAL
·
2025
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IF 13.2
Lv Xi,Xu Xincheng,Peng Yang,Li Bolin,Yang Lie,He Xiaoman
This study introduces a novel modification method for micron-sized zero-valent iron, utilizing Shewanella oneidensis MR-1 to simultaneously colonize its surface, disrupt the passivation layer, and alter the crystal structure of the core. This process yields a composite that retains its reducing activity even in high concentrations of pollutants (500 ppm Cr(VI)) and under neutral to slightly acidic conditions. Our findings indicate that the modified system induces the Kirkendall effect in zero-valent iron, leading to the formation of amorphous zero-valent iron with a non-spherical core structure, in contrast to conventional direct mixing approaches. The modified system achieves complete reduction of 100 ppm Cr(VI) to Cr(III) within 8 h. Notably, within the first 4 h of reaction, the reduction rate is approximately three times higher than that of the conventional direct mixing system. XPS, XRD, SEM, EEM and TEM analyses reveal that the surface passivation layer of the biochemically modified zero-valent iron is disrupted, leading to changes in its crystal structure, the formation of hollow spherical structures, and the development of a biological protective film on its surface. Interestingly, electrochemical and ETSA analyses indicate that the electron transfer rate of the biochemically modified composite is significantly enhanced. This study provides fundamental insights into the removal of Cr(VI) from groundwater using biochemically modified micron-sized zero-valent iron (mZVI).
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ACS Applied Materials & Interfaces
·
2025
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IF 8.2
Juntao Huang,Saifei Wang,Zhifei Zhang,Mingli Chen,Han Wang,Haihua Huang,Qian Wang,Jigong Hao,Wei Li,Juan Du,Mahesh Kumar Joshi,Peng Fu
Ceramic dielectric capacitors have gained significant attention due to their ultrahigh power density, current density, and ultrafast charge–discharge speed. However, their potential applications have been limited by their relatively low energy storage density. Researchers have employed various approaches to enhance their energy storage density. In this study, (1 – x)(Bi0.5Na0.5)0.7Sr0.3TiO3–xCa(Mg1/3Ta2/3)O3 ceramics were prepared via a solid-phase reaction, and the effect of their structure on the energy storage properties was investigated. The results indicate that the introduction of Ca(Mg1/3Ta2/3)O3 significantly alters the multiscale structures of the (Bi0.5Na0.5)0.7Sr0.3TiO3 ceramic, including the transformation from the T-phase to the C-phase, refinement of ceramic grains, and the formation of polar nanoregions (PNRs), accompanied by an increase in the bandgap and relaxation degree. These structural changes collectively contributed to the improved overall energy storage properties of the modified ceramics. Notably, the 0.92(Bi0.5Na0.5)0.7Sr0.3TiO3–0.08Ca(Mg1/3Ta2/3)O3 ceramic demonstrated a recoverable energy storage density (Wrec) of 8.37 J/cm3 with an energy storage efficiency (η) of 87.7% at an electric field of 530 kV/cm. It also exhibited good temperature stability (25–120 °C), frequency stability (1–100 Hz), and fatigue stability (1–105). Furthermore, it displayed exceptional charge and discharge properties, with the discharge energy density (WD), discharge time (t0.9), current density (CD), and power density (PD) attaining 4.06 J/cm3, 35.2 ns, 2143.28 A/cm2, and 460.71 MW/cm3, respectively. These findings suggest that modified (1 – x)(Bi0.5Na0.5)0.7Sr0.3TiO3–xCa(Mg1/3Ta2/3)O3 ceramics are promising candidates for high-power pulsed electronic systems.
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Journal of Environmental Chemical Engineering
·
2025
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IF 7.2
Mengyao Gui,Hongwei Yang,Erming Ouyang
Polystyrene nanoplastics (NPs), ubiquitous contaminants in aquatic ecosystems, are known to impede conventional anoxic denitrification processes. However, their effects on aerobic denitrification systems remained poorly understood. This study systematically elucidates the mechanistic interactions between polystyrene NPs and aerobic denitrification performance. Results revealed polystyrene NPs caused concentration-dependent suppression of denitrification efficiency in the aerobic denitrifier Pseudomonas stutzeri PCN-1 under controlled laboratory conditions (aerobic, 30 ℃). At NPs concentrations escalating from 100 to 1000 μg L⁻¹ , nitrate removal rates decreased markedly (0.4–3.3 mg L⁻¹ h⁻¹), paralleled by progressive accumulation of nitrite (8.8–87.2 mg L⁻¹) and N₂O (31.2–58.3 mg L⁻¹). Mechanistic analyses revealed that polystyrene NPs induced biotoxic effects through two primary pathways: (1) disruption of cellular membrane integrity and (2) excessive generation of reactive oxygen species (ROS), collectively impairing bacterial viability and metabolic activity. Furthermore, critical denitrification processes were compromised, including diminished electron transport system activity, reduced catalytic activities of key denitrification enzymes, and downregulation in transcriptional expression of functional genes (e.g. napA , nirS , cnorB , and nosZ ). These multilevel inhibitions collectively contributed to the observed deterioration in nitrogen removal capacity. This investigation provides novel insights into the ecotoxicological risks posed by NPs to microbial nitrogen cycling in aquatic environments.
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Frontiers in Microbiology
·
2022
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IF 6.064
Chenrui Peng,Jinxing Li,Zhonghua Miao,Yunyi Wang,Simou Wu,Yimei Wang,Silu Wang,Ruyue Cheng,Fang He,Xi Shen
Inflammatory bowel disease (IBD) is a chronic intestinal disease characterized by microbiota disturbance and intestinal mucosal damage. The current study aimed to investigate the preventive effects of Bifidobacterium bifidum BD-1 (BD-1) against long-term IBD and possible mechanism by which it alters the gut microbiota, immune response, and mucosal barrier. Our study found that early treatment of BD-1 + Ceftri (ceftriaxone followed by BD-1) and BD-1 confers a certain protective effect against the occurrence of long-term Dextran sulfate sodium-induced colitis, which manifests as a decrease in inflammation scores and MPO activity levels, as well as a relatively intact intestinal epithelial structure. Moreover, compared to BD-1, Ceftri, and NS, early treatment with BD-1 + Ceftri promoted greater expression levels of mucosal barrier-related proteins [KI67, MUC2, ZO-1, secretory immunoglobulin A (slgA), Clauding-1, and Occludin], better local immune responses activation, and moderately better modulation of systemic immune responses during long-term colitis. This may be due to the fact that BD-1 + Ceftri can deliberately prolong the colonization time of some beneficial microbiota (e.g., Bifidobacterium ) and reduce the relative abundance of inflammation-related microbiota (e.g., Escherichia/Shigella and Ruminococcus ). Interestingly, we found that the changes in the gut barrier and immunity were already present immediately after early intervention with BD-1 + Ceftri, implying that early effects can persist with appropriate intervention. Furthermore, intervention with BD-1 alone in early life confers an anti-inflammatory effect to a certain degree in the long-term, which may be due to the interaction between BD-1 and the host’s native gut microbiota affecting intestinal metabolites. In conclusion, BD-1 was not as effective as BD-1 + Ceftri in early life, perhaps due to its failure to fully play the role of the strain itself under the influence of the host’s complex microbiota. Therefore, further research is needed to explore specific mechanisms for single strain and native microbiota or the combination between probiotics and antibiotics.
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