FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Xiaowei Ding,Xuerong Luo,Wenting Shi,Dekun Cheng,Xiang He,Lin Yue,Zhouping Wang
Multi-functional film materials that enable monitoring and control of food safety are effective tools for addressing food safety issues. In this study, nitrogen-doped citric acid carbon dot (NCD) functionalized with surface potassium ions was incorporated into chitosan film to confer film pH-monitoring capability. The inclusion complex (HPCDGer) of hydroxypropyl cyclodextrin (HPCD) and geraniol (Ger) was prepared and added as antimicrobial material to the chitosan film. With the increase in pH, the fluorescence intensity of the composite film increased significantly. CS/NCD@HPCDGer exhibited excellent UV shielding, water vapor barrier, antioxidant (Scavenging activity of DPPH and ABTS were 74.01% and 92.48%, respectively), and antimicrobial effects. The inhibition rates against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were 99.82% and 95.89%, respectively. The pH, weight loss, and total volatile alkaline nitrogen (TVB-N) of the pork packed in the composite film were significantly lower. Furthermore, the composite film is capable of showing orange fluorescence in response to biogenic amines from pork spoilage. These results indicate that the composite film has potential applications in the monitoring and control of food safety.
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Molecular Catalysis
·
2024
·
IF 3.9
Qiqi Xing,Xiaoyu Wang,Qi Li,Changning Lu,Yi Wu,Jianjun Pei,Linguo Zhao
β-Galactosidase is a crucial class of biocatalysts, renowned for its ability to catalyze the hydrolysis of non-reducing terminal β-d-galactoside bonds, while also demonstrating significant potential in galactosylation synthesis reactions. In this study, a novel GH1 β-galactosidase gene VthBga was screened and cloned from Vulcanisaeta thermophila . Soluble expression of the VthBga was induced at 47 °C in Escherichia coli BL21. VthBga exhibited optimal activity at 95 °C and pH 4.5 with p -nitrophenyl-β-d-galactoside ( p NPGal) as a substrate and displayed excellent thermotolerance below 90 °C. In addition, VthBga possessed not only a diverse array of substrate hydrolytic activities but also inherent galactosylation activity, rendering it a promising candidate for the galactosylation of the natural compound. To enhance its transglycosylation activity, a superior mutant Vth-A266G/F441T was obtained by adjusting the transglycosylation/hydrolysis ratio through a semi-rational design. Under optimal conditions, this mutant produced 44.5 % tyrosol galactoside, representing a significant 2.3-fold increase compared to the wild-type strain, highlighting its practical value in related biosynthesis applications.
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Food Bioscience
·
2024
·
IF 4.8
Jun Lv,Chang Liu,Pan-Pan Tang,Jun-Xiang Liu,Rana Muhammad Aadil,Jun-Hu Cheng,Shahzad Hussain,Xiu-Bin Liu,Zhi-Wei Liu
The application of cold plasma (CP) treatment was investigated for its effectiveness in promoting the covalent grafting of β-lactoglobulin (β-LG) and epigallo-catechin 3 -gallate (EGCG), as well as improving the antioxidant capacity of β-LG and eliminating its antigenicity. The results indicated that CP treatment significantly enhanced the covalent grafting of β-LG and EGCG. The maximum EGCG content in β-LG-EGCG complexes reached 47.08 ± 0.58 mg/g (with 45 s treatment), as confirmed by SDS-PAGE analysis and FTIR spectroscopy. Additionally, LC-MS/MS identifies two binding sites (T76 and K77) of EGCG in the β-LG-EGCG conjugates. After 60 s treatments, there was a significant reduction in the IgG binding capacity of β-LG-EGCG conjugates (29.97 ± 0.05%). Further analysis suggested that the change in the conformational epitopes of β-LG, due to the conjugating with EGCG, was likely the main factor responsible for the reduced binding Immunoglobulin G (IgG) capacity. The maximum antioxidant activities of β-LG-EGCG conjugates were found to be 974.14 ± 1.94 μmol TE g −1 for ABTS and 748.48 ± 9.66 μmol TE g −1 for DPPH). These values represent an eightfold increase in DPPH activity and a twofold increase in ABTS compared to the control sample. This study demonstrated that the CP treatment is highly effective technology for facilitating the covalent grafting of polyphenols to proteins, thereby enhancing their antioxidant properties and eliminate their antigenicity.
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JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Fanjin Meng,Xin Ju,Yuyang Zhou,Lishi Yan,Zhi Chen,Yuqiu Wu,Liangzhi Li
The two major impediments to biomass resourcing are the catalytic efficiency of enzymes and the effectiveness of biomass pretreatment. In this study, six genes encoding β-Glucosidases (BGLs) derived from Paenibacillus sp. LLZ1 were cloned and expressed. The activity of BGL-F, which had the highest enzyme activity, was 24.1 U/mg, which was 39.8–83.7% higher than that of the other BGLs. Molecular docking and surface charge analyses showed that the binding energy of BGL-F to cellobiose and its electronegative ligand-binding pocket were greater than those of other BGLs. On the other hand, by calculating and comparing the Kamlet–Taft (KT) values of deep eutectic solvent (DES) composed of different hydrogen bond donors (HBD) and hydrogen bond acceptors (HDA), it was found that DES composed of betaine and glycerol had the highest α value of 0.97, meaning that it could provide the highest hydrogen bonding capacity, and thus it had the best pretreatment effect on biomass. Analysis by circular dichroism (CD) spectroscopy revealed a decrease in α-helix and an increase in β-strand in the secondary structure of the DES-treated BGL-F, implying an increase in enzyme activity and stability. This result was also confirmed experimentally by the fact that the K m and V max of the enzyme in the DES system increased by 13.2% and 57.9%, respectively, and the inactivation rate (k d ) decreased by 21.4%. The results of this study showed that DES consisting of glycerol-betaine achieved a better balance between the promotion of enzyme activity and biomass pretreatment.
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Journal of Materials Chemistry B
·
2015
·Vol. 3
·期 26
·5277-5283
·
IF 4.726
Shuling Yu,Jintao Yuan,Jiahua Shi,Xiaojiao Ruan,Yali Wang,Shufang Gao,Yu Du
A small, nano-sized, water-soluble polyrotaxane (PR) was synthesized using a highly efficient one-pot synthesis strategy in a homogeneous water system, formed from β-cyclodextrin-(COOH)2, poly(propylene glycol)bis(2-aminopropyl ether) (PPG, 2 kDa) and a mono-(6-azido-6-desoxy)-β-cyclodextrin stopper via room temperature click chemistry. β-cyclodextrin-(COOH)2 and PR were characterized by one- and two-dimensional NMR as well as by high resolution transmission electron microscopy (HR-TEM). The number of carboxyl groups in one PR was determined by 1H NMR. Two-dimensional diffusion-ordered NMR spectroscopy (2D DOSY) and nuclear Overhauser enhancement spectroscopy (2D NOESY) show that β-cyclodextrin-(COOH)2 and PPG successfully formed an inclusion complex. HR-TEM revealed the morphology of water-soluble PR as a spherical nanoparticle with a size of approximately 3.5 nm ± 1.5 nm. PR was labeled with rhodamine to assess its biocompatibility and cell membrane penetrability in vitro. The in vivo real-time fluorescent imaging biodistribution experiments indicated that water-soluble PR can actively target tumor sites using an enhanced permeability and retention (EPR) effect, with a significantly prolonged blood circulation time in tumor-bearing mice.
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PubMed
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Lin Gao,Luping Gu,Wentian Li,Gang Wang,Jianxin Zhao,Wei Chen,Peijun Tian
5-Hydroxytryptophan (5-HTP) is a functional food ingredient widely recognized for its sleep-enhancing properties, but it has a short elimination half-life in vivo , resulting in suboptimal pharmacokinetic performance. In this study, we developed a composite nanoparticles system based on β-cyclodextrin and corn starch using the anti-solvent precipitation method for the encapsulation and sustained release of 5-HTP. The nanoparticles were characterized using laser particle size analysis, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and evaluation of their digestive properties in simulated gastrointestinal fluids. We further evaluated their effects on the pharmacokinetic profile and distribution of 5-HTP in rats, including serum, gut, and brain, demonstrating a significant extension of the drug elimination half-life and improved bioavailability. In an insomnia mouse model, administration of the composite nanoparticles system significantly alleviated behavioral abnormalities and circadian rhythm disorder induced by sleep deprivation. This study provides a solid theoretical basis and technical support for the efficient application of 5-HTP in functional foods.
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PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS
·
2016
·
IF 3.155
Jihui Wang,Yuan Liang,Luyang Jian,Jingwei Zhang,Shuai Liang,Shan Xiao,Bingnan Liu,Han Wang
Obesity has become a major health problem which is related with high-trans fatty acids diet. Adipogenic differentiation of adipose tissue-derived stromal cells (ADSCs) plays an important role in the development of adipose tissue . In order to determine the effect of trans fatty acids on adipogenic differentiation in ADSCs, cells were treated with linoelaidic acid, as well as linoleic acid and linolenic acid . We found that linoelaidic acid significantly increased the lipid droplet formation and triglyceride content compared with linoleic acid and linolenic acid. Linoelaidic acid also down-regulated the levels of β-catenin in cells and inhibited the accumulation of β-catenin in cell nuclei . Lithium chloride , an activator of Wnt/β-catenin pathway, antagonized the enhancement of linoelaidic acid on adipogenesis and up-regulated the levels of β-catenin in ADSCs. These results indicated that linoelaidic acid could enhance the adipogenic differentiation in ADSCs in vitro , which is partly due to the suppression of Wnt/β-catenin pathway.
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PubMed
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CARBOHYDRATE RESEARCH
·
2016
·
IF 1.817
Hui Zhao,Jun Gao,Ruina Liu,Sanping Zhao
To fabricate thermo- and pH-sensitive hydrogels functionalized with β -cyclodextrin ( β -CD) moieties, β -CD polymer bearing methacrylate (CDP- g -GMA) used as a reactive and functional crosslinker was synthesized, and then copolymerized with N -isopropylacrylamide (NIPAAm) and acrylic acid (AA) in aqueous solution via UV-initiated free radical polymerization. The stimulus-responsiveness of the resultant hydrogels has been carried out by measuring the swelling ratio at different temperatures and pH values. The results showed that the thermo- and pH-sensitivities of the produced hydrogels were significantly dependent on the compositions of the hydrogels, and the dual sensitivities exhibited good reversible process. The interior morphology observed by SEM exhibited that the pore size of the hydrogels could be tailored by pH of the local medium. Using a water-soluble cationic dye methyl violet (MV) as a model drug, MV loading and release profiles of the hydrogels as potential drug controlled release carriers were evaluated. The MV release rate from CD-functionalized hydrogels was much slower than that from the hydrogel without β -CDs at both pH 2.0 and pH 7.4. The release of MV from CD-functionalized hydrogels at pH 2.0 was faster than that at pH 7.4, the release kinetics of MV from the CD-functionalized hydrogels displayed a sustained release profile, and the release mechanism followed Fickian diffusion.
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POLYMER BULLETIN
·
2016
·Vol. 74
·期 4
·1091-1101
·
IF 1.371
Li Hu,Zhou Zhen,Liu Jingcheng,Xu Wenjia,Liu Ren,Liu Xiaoya
Positive chemically amplified molecular glass with pendant t -butyloxy carbonyl ( t -BOC) groups based on β-cyclodextrin (β-CD) was prepared. The β-CD derivatives were obtained from the reaction of β-CD and di- tert -butyl dicarbonate (DBDC) in the presence of 4-dimethylaminopyridine, and the protecting ratio was controlled using various feed amounts of DBDC. Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance were employed to characterize the chemical structure, indicating that the synthesis was successful. Thermal behavior was characterized by thermogravimetric analysis; the results showed that the initial thermal decomposition temperature occurred at approximately 160 °C, which could satisfy the lithography process. Ultraviolet–visible spectrophotometer indicated that the t -BOC-protected β-CD derivatives had low absorbance at 365 nm wavelength. Additionally, X-ray diffraction analysis results showed that the β-CD derivatives had amorphous form, and exhibited excellent film-forming property. The photoresists consisting of different t -BOC protecting groups showed high sensitivity when exposed to 365 nm light and post-baked at 105 °C for 90 s, followed by developing in 2.38 wt% aqueous tetramethylammonium hydroxide solution at room temperature. An enhanced line and space positive patterns with 1 μm resolution were delineated according to digital microscope.
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JOURNAL OF CHROMATOGRAPHY A
·
2016
·
IF 3.926
Lei Tan,Rong He,Yongxian Li,Yong Liang,He Li,Youwen Tang
α-Amanitin and β–amanitin are the main toxins of mushroom poisoning. The application of traditional non-selective adsorbents is not satisfactory in clinical treatment of amanita mushroom poisoning due to lack of specificity adsorption capability of these adsorbents toward amanitin toxins. In the current work, we introduce a novel molecularly imprinted biomimetic adsorbent based on a ligand specificity determinant through surface imprinted strategy. Owing to the expensive price of the amanitin sources, we selected a typical common moiety of α, β–amanitin as specificity determinant to synthesize a template necessary for the preparation of molecularly imprinted polymers (MIPs). Computer simulation was used to initially select acidic methacrylic acid (MAA) and basic 4-vinyl pyridine (4-VP) together as functional monomers. The experiments further demonstrated that the synergistic interaction of MAA and 4-VP played a primary role in the recognition of α, β-amanitin by MIPs. By means of batch and packed-bed column experiment and the hemocompatibility evaluation, the resultant biomimetic adsorbent has been proved to be capable of selectively removing α, β–amanitin and possess good hemocompatibility. This novel adsorbent has great potential to find application in human plasma purification.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2016
·Vol. 20
·期 5
·1263-1270
·
IF 2.327
Wang Lili,Yu Feiyue,Wang Fang,Chen Zilin
We describe a method for detecting DNA methylation. It is based on direct oxidation of DNA bases at a glassy carbon electrode (GCE) modified with film of a multiwalled carbon nanotube-β-cyclodextrin composite. This nano-structured film causes a strong enhancement on the oxidation current of DNA bases due to its large effective surface area and extraordinary electronic properties. Well-defined peaks were obtained as a result of electro-oxidation of guanine (at 0.67 V), adenine (at 0.92 V), thymine (at 1.11 V), cytosine (at 1.26 V), and 5-methylcytosine (at 1.13 V; all data vs. saturated calomel electrode (SCE)). The potential difference between 5-methylcytosine and cytosine (130 mV) is large enough to enable reliable simultaneous determination and analysis. The interference by thymine can be eliminated by following the principle of complementary pairing between purine and pyrimidine bases in DNA. The modified electrode was successfully applied to the evaluation of 5-methylcytosine in a fish sperm DNA, the methylation level of cytosine was found to be 7.47 %, and the analysis process took less than 1 h.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Zhiming Jiang,Guangyu Li,Mingxiao Zhang
A novel electrochemical sensor for gatifloxacin based on one-pot electro-polymerization of β-cyclodextrin (β-CD) and reduced-graphene oxide (rGO) modified glassy carbon electrode (GCE) (β-CD/rGO/GCE) was built for the first time. Scanning electron microscope (SEM) image and Infrared spectroscopy (IR) show that polymer of β-cyclodextrin and reduced graphene oxide (rGO) has been successfully modified on electrode. The electrochemical properties of this polymer were characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The optimization of the electro-polymerized cycle, the pH of supporting electrolyte and the accumulation potential and time were discussed in details. Under optimum conditions, the differential pulse voltammogram (DPV) exhibited the oxidation peak currents were linearly proportional to gatifloxacin concentrations in the range from 0.05 μM to 150 μM with a sensitivity of 0.33 μA μM −1 . This electrochemical sensor had a low detection limit of 0.02 μM ( S/N = 3). Moreover, this proposed sensor exhibited good reproducibility, long-term stability and fast current response. To further study the practical applicability of the proposed sensor, the modified electrode was successfully applied to detect gatifloxacin in tablets and human urine samples.
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