Communications Biology
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2026
·
IF 5.8
Kunpeng Jia,Yuxin Ding,Menglei Jin,Qi Yang,Xinyu Wang,Yujie Yan,Jiao Xiang,Ruoqi Zhang,Jun Fang,Jingxin Jiang,Danting Wang,Fengbo Huang,Jianming Hu,Jian Huang,Yeting Hu,Zhigang Chen
Chemotherapy resistance remains a major challenge in advanced colorectal cancer (CRC), with chemotherapy-induced senescence playing a key role. While cancer cell senescence has been extensively studied, the contribution of stromal components, particularly vascular endothelial cells, remains unclear. Here, we analyze 77 CRC patients undergoing neoadjuvant chemotherapy and find that favorable responders exhibit well-defined vascular lumens, whereas poor responders show an increase in senescent endothelial cells (senEndo), as revealed by single-cell transcriptomics and immunofluorescence. Chemotherapy-induced senescence reduces GPX4 ubiquitylation in senEndo, leading to its accumulation and extracellular vesicle transfer to CRC cells, where it inhibits ferroptosis and promotes chemoresistance. In vitro and in vivo models demonstrate that targeting GPX4 with the ferroptosis activator RSL3 restores chemotherapy sensitivity. These findings identify a novel mechanism of chemoresistance mediated by senEndo-derived GPX4 in extracellular vesicles and highlight the potential of ferroptosis induction, alone or in combination with senolytic agents, to improve 5-Fu/oxaliplatin-based CRC therapies. Extracellular vesicles from senescent endothelial cells transfer GPX4 to colorectal cancer cells, suppress ferroptosis, and promote resistance to neoadjuvant chemotherapy
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EMBO JOURNAL
·
2026
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IF 8.4
Niu Sheng,Tian Yuyang,Nguyen Linh,Bai Chongzhi,Hou Xiaohan,Wang Dan,Niu Ziqian,Liu Zhimin,Ren Jianle,Li Wentao,Wang Qihui,Tian Wen-xia,Sun Junqing,Gao George Fu
Type-II feline infectious peritonitis virus (FIPV-II) is a lethal alphacoronavirus, whose high homology with the human coronavirus CCoV-HuPn-2018 carries the risk of potential zoonotic FIPV-II transmission to humans. FIPV-II infects felids using cat aminopeptidase N (cAPN) as its cell-entry receptor, but the molecular details remain unclear. Here, we resolved cryo-electron microscopy (cryo-EM) structures of the spike (S) trimer of a representative FIPV-II strain 79-1146 (FIPV-1146), and of its complex with cAPN. The reconstructions reveal structural transitions of the S protein between the “standing” and “lying” receptor-binding domain (RBD) conformation upon complex formation with cAPN in its open conformation. Structural and mutational analyses revealed that the cAPN residues R378, D751 and R779, as well as the N748-linked glycan are essential for high-affinity RBD engagement. Cross-species analysis demonstrated narrow tropism of FIPV-1146, limited to felids and canids. Introducing the N595K and Q596R substitutions found in transmissible gastroenteritis virus (TGEV) into the FIPV-1146 RBD expands its binding capacity to APNs from pig, bovine, horse and giant panda. These findings provide insights into the entry mechanism and zoonotic constraints of FIPV-II, with potential relevance for antiviral strategies against coronaviruses.
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EMBO JOURNAL
·
2026
·Vol. 45
·期 13
·4670-4693
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IF 8.4
Feng Xuanjun,Zhang Weixiao,Guan Huarui,Yang Haolan,Wu Na,Chen Yuting,Mao Xin,Xiao Huihui,Li Mengling,Xiong Hao,Li Yinzhi,Zhang Zhaoyu,Liu Muyan,Jia Li,Cha Shuang,She Yizhen,Zhang Xuemei,Liao Haicheng,Sun Guangchao,Liu Yaxi,Wang Wenming,Xu Yunbi,Zhang Yuelin,Lu Yanli
SUMOylation is critical for plant growth and defense, and the specific substrate recognition mediated by SUMO ligases dictates the biological processes in which SUMOylation functions. However, only four SUMO ligases involved in SUMOylation have been reported in plants. Here, we report MPEL1 as a new SUMO ligase in maize, which paradoxically exhibits SUMO ligase activity despite its sequence homology to SUMO-targeted ubiquitin ligases (STUbL). MPEL1 stabilizes the plant-specific mitogen-activated protein kinase 16 (MAPK16) via SUMOylation at Lys526 within C-terminal domain (CTD). MAPK16 directly binds to JAZ20 via its C-terminal domain and phosphorylates JAZ20 at Thr12/Ser13 residues, thereby triggering its proteasomal degradation and activating broad-spectrum resistance against two Fusarium pathogens. These pathogens cause devastating maize ear rot and stalk rot, leading to severe yield losses and serious food safety concerns worldwide. Notably, MAPK16 overexpression and JAZ20 knockout enhance disease resistance without yield penalty, highlighting their potential for crop improvement. These findings expand the plant SUMO ligase family and provide targets for breeding Fusarium-resistant crops.
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RESPIRATORY RESEARCH
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2026
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IF 5.7
Zhou Weitao,Dai Dan,Chen Yao,Han Xiao,Dong Xinran,Liao Yunfei,Chen Tong,Wang Huixian,He Chen,Lu Yilei,Zheng Chenxin,Zhou Yufeng,Qian Liling
Biallelic isoleucyl-tRNA synthetase 1 ( IARS1 ) variants are associated with an IARS1 -related phenotype characterized by growth retardation, intellectual disability, muscular hypotonia, infantile hepatopathy, and interstitial lung disease. This rare, early-onset disorder is often lethal. Although several variants have been identified in recent years, their pathogenicity and underlying molecular mechanisms remain poorly understood. In this study, we generated Iars1 Y148C/R444* compound heterozygous mice, which exhibited pulmonary alveolar proteinosis - like lesions, growth retardation, and muscular hypotonia. Lipid metabolomic profiling of these mice revealed dysregulation of cholesterol metabolism pathways. Furthermore, the Iars1 mutation increased surfactant deposition in alveolar macrophages by impairing lysosomal degradation and disrupting lysosome-autophagosome fusion. We also found that partial loss of IARS1 activity interfered with the post-translational processing of cathepsin Z by inhibiting its ubiquitin-mediated degradation. Importantly, exogenous expression of cathepsin Z improved surfactant clearance in macrophages with partial loss of Iars1 activity. Collectively, our findings demonstrate that partial loss of IARS1 activity induces lysosomal dysfunction in alveolar macrophages and promotes pulmonary surfactant accumulation in the alveoli, thereby contributing to the development of pulmonary alveolar proteinosis.
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Nature Aging
·
2026
·
IF 25
Wu Meng,Zhu Qingqing,Xiong Jiaqiang,Tang Weicheng,Chen Dan,Xue Liru,Feng Yourong,Dai Yun,Wu Tong,Wu Chuqing,Guo Yican,Wei Simin,Huang Yibao,Zheng Peizhe,Li Yuting,Song Yiqing,Ding Ting,Wu Mingfu,Li Zifu,Rong Yueguang,Wang Wenwen,Li Yan,Dai Jun,Wang Shixuan,Zhang Jinjin
Recent studies have highlighted the crucial role of mechanical properties in the ovarian microenvironment for ovarian function. However, the mechanisms that cause ovarian matrix stiffening during aging remain incompletely understood. Here we utilized atomic force microscopy (AFM) to demonstrate that human ovarian matrix stiffness increases with aging and in pathophysiological conditions, such as chemotherapy-induced premature ovarian insufficiency (POI), polycystic ovary syndrome (PCOS) and ovarian endometriosis. By integrating proteomic analysis of human ovarian tissue with transcriptomic profiling of human ovarian fibroblasts, we identified that IL-11, which is elevated in aging ovaries of mice, rats and humans, activates fibroblasts to secrete extracellular matrix (ECM), thereby increasing ovarian matrix stiffness. Genetic deletion of Il11ra1 in mice mitigated the increase in ovarian matrix stiffness and the decline in ovarian function associated with aging, chemotherapy-induced POI and PCOS. Single-nuclei RNA sequencing (snRNA-seq) revealed that blocking Il11r a 1 reduces the proportion of activated fibroblasts. Furthermore, administration of si Il11 nanoparticles to aged mice and rats enhanced fertility and reduced ovarian matrix stiffness. Together, these findings highlight the pro-inflammatory factor IL-11 in regulating ovarian matrix stiffness. We propose that anti-IL-11 therapy represents a promising translational strategy for delaying ovarian aging.
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Nature Chemistry
·
2026
·
IF 24.5
Wang Nan,Cui Chunyu,Xu Runjing,Wang Xiaofang,Wu Chaoxin,Pu Qihao,Wang Wentao,Cao Yongjie,Liao Mochou,Wang Fengmei,Wang Yonggang,Chen Yuhui,Ji Xiao,Xia Yongyao,Wang Fei
Magnesium–oxygen (Mg–O 2 ) batteries are considered promising candidates for next-generation energy storage systems due to their high specific energy, low cost and intrinsic safety. However, poor rechargeability remains a critical barrier, largely due to the lack of suitable electrolytes and undesired irreversible cathode reactions. Here we report a rechargeable non-aqueous Mg–O 2 battery enabled by a tailored electrolyte featuring a cage-like Mg 2+ solvation environment. We find that varying ether solvent chain lengths, alongside a tridentate chelating agent, can act coordinately to form a dynamic solvation microreactor. This solvation environment is consistent with the reversible formation of nanocrystalline magnesium peroxide at the cathode and remains compatible with reversible magnesium plating/stripping. The resulting Mg–O 2 batteries achieve a high initial discharge voltage approaching 2.0 V, an ultralow overpotential of 0.35 V, a remarkable round-trip energy efficiency of 80% and over 450 stable cycles. These results highlight a solvation-structure-guided strategy for enabling rechargeable Mg–O 2 batteries and potentially other multivalent metal–air systems.
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Journal of Thermal Science
·
2026
·Vol. 35
·期 4
·1342-1354
·
IF 3.2
Hao Yunze,Gong Yutong,Liu Feng,Lv Yanlong,Wang Yuhao,Li Jinping
In response to increasing energy demands, absorption heat transformers (AHTs) have been widely employed as efficient energy conversion devices across various fields. However, existing working pairs (e.g., LiBr/H 2 O and NH 3 /H 2 O) exhibit high corrosiveness and limited thermal stability, significantly restricting their applicability in high-temperature environments. Therefore, the development of high-performance working pairs is crucial for improving the efficiency and stability of AHT systems. In this study, four groups of ionic liquid-based ternary matrix pairs: LiBr-[EMIM]Cl/H 2 O (1:2), LiBr-[EMIM]Cl/H 2 O (1:3), LiBr-[EMIM][DEP]/H 2 O (1:3), and LiBr-[EMIM][DMP]/H 2 O (1:3), were systematically evaluated through short-term corrosion experiments. The effects of corrosiveness, thermal stability, density, viscosity, and specific heat capacity were analyzed. Experimental results and data analysis clarified the high-temperature properties of ionic liquids, while thermogravimetric analysis and viscosity measurements were conducted to evaluate the relative merits of different working pairs. Results indicate that the LiBr-[EMIM]Cl/H 2 O (1:2) system exhibits the lowest corrosiveness and good thermal stability, making it suitable for corrosion-sensitive AHT systems, while the LiBr-[EMIM][DMP]/H 2 O (1:3) system demonstrates optimal thermal stability at high temperatures, rendering it a complementary solution for high-temperature applications. This study establishes an experimental basis for optimizing AHT working pairs and provides essential data to inform their selection in AHT systems.
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Scientific Reports
·
2026
·
IF 4.9
Zhang Guangteng,Chen Yadong,Wang Meichen,Ge Da,Du Qiang,Ke Li,Gong Tianxing,Qi Feng,Bao Juncheng
Hydrogels are widely used in tissue engineering and scaffold fabrication due to their excellent biocompatibility, while 3D printing excels at creating complex architectures. Among various techniques, low-temperature extrusion-based 3D printing has shown promise for hydrogel scaffold fabrication, as rapid solidification under controlled cooling can improve shape retention during deposition. However, commercial low-temperature DIW systems are often expensive, closed-source, and difficult to customize. Moreover, most require a two-step post-processing workflow involving external freeze-thaw cycles, which can damage structures through mechanical disturbance and temperature gradients, increasing contamination risk. To address these issues, we developed a low-cost, open-source low-temperature 3D printing platform by modifying a commercial FDM printer with custom hardware and upgraded software. It maintains stable − 30 °C ± 1 °C and supports in-situ freezing and freeze-thaw processing. Using a Poly(vinyl alcohol)-lignosulfonate sodium-TEMPO-oxidized cellulose nanofibrils (PVA-LS-TOCNF) hydrogel as the model ink, we achieved stable printing of various patterns and mechanical test specimens, with tensile properties comparable to those from ex-situ freeze-thaw methods. Overall, this platform significantly lowers barriers to low-temperature hydrogel 3D printing and improves accessibility.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2026
·
IF 4.6
Long Yu-Si,Lu Mei-Yi,Qi Jia-Wei,Chen Geng,Sun Yu,He Xiu-Xia,Zhang Tian-Hui,Cui Li-Guo,Hu Jun-Li,Liu Yi-Chun
Silk fibroin hydrogels frequently exhibit insufficient mechanical strength for biomedical applications. Conventional enhancement strategies often rely on toxic crosslinkers or nondegradable components, thereby compromising biocompatibility and biodegradability. Recently emerged organic solvent-based methods represent an advance, but their associated environmental toxicity remains a concern. In this work, we present a novel thermal oscillation strategy for fabricating high-strength silk fibroin hydrogels exclusively from all-aqueous silk fibroin solutions, eliminating the need for toxic chemicals or non-degradable materials. The resulting hydrogels exhibit outstanding mechanical properties, tensile strength of 2.6 MPa, elongation rate of 152.6%, and toughness of 3.0 MJ/m 3 , which not only surpass the organic solvent-derived silk fibroin hydrogels but also rival the dual-crosslinked, double-networked or composite systems. The silk fibroin hydrogels also exhibit a minimal swelling ratio, outstanding long-term integrity, and excellent biocompatibility. The robust, biocompatible, and biodegradable silk fibroin hydrogels are promising for biomedical applications such as load-bearing tissue scaffolds, long-term implantable drug delivery systems, and durable wound dressings.
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Nature Communications
·
2026
·
IF 18.1
Tang Linbin,Lu Zechun,Gao Zongyan,Lou Xuechun,Li Junjian,Wen Yujie,Chen Junchao,Zhu Zhexuan,Luo Shijing,Zhou Lina,Wei Guangfeng,Chen Zuofeng,Zhao Hongying,Li Tong,Peng Luming,Li Fengting,Liu Tao
Achieving a reversible four-electron-per-oxygen-molecule oxygen evolution reaction is an essential yet highly challenging task for nonaqueous lithium hydroxide-based Li | |O 2 batteries, as the kinetically sluggish oxygen evolution reaction tends to entangle with competing parasitic reactions, whose origins and mitigating strategies remain largely elusive. Here we construct a highly reversible lithium hydroxide-based Li | |O 2 battery using iron–cobalt–nickel layered double hydroxide catalysts and tetramethylene sulfone-based electrolytes. Lithium hydroxide decomposition toward oxygen evolution involves key reactive oxygen species of surface-bound hydroxyl and hydroxyl radicals, but no singlet oxygen. These hydroxyl species corrode the electrolyte and carbon support, predominantly accounting for charging irreversibility. While oxidation-resistant solvents and electrical conductors are necessary to reduce hydroxyl-induced side reactions, the synergistic interplay of interfacial water solvation and catalytic surface structures hold the key to steering hydroxyl activity toward the desirable oxygen evolution reaction or by-product formation. This work offers insights into achieving long-life lithium hydroxide-based Li | |O 2 batteries.
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Nature Communications
·
2026
·
IF 18.1
Wang Guojuan,Xiong Wei,Qiu Linrong,Hong Chengwu,Liu Ye,He Yutong,Li Shuxing,Xie Rong-Jun
Laser-driven light sources are critically important for lighting, display, and communication systems. However, the traditional phosphor ceramics used in these light sources suffer from low light conversion efficiency, low color rendering index and narrowly tunable color temperature. Here we present a approach of rapid pulsed high-temperature sintering technology to fabricate phosphor ceramics with both dense and grain refined microstructure simultaneously. It results in a 31% improvement in light conversion efficiency, markedly better color mixing uniformity, and 1.5-fold increased fracture toughness for Lu 3 Al 5 O 12 :Ce ceramics. Moreover, this method enables the Lu 3 Al 5 O 12 :Ce–CaAlSiN 3 :Eu composite ceramics with a 95-fold enhancement in quantum efficiency, showing white light under blue laser excitation with a color rendering index of 93 and good reliability. An accurate emission wavelength prediction model was also established with an interval below 5 nm from 495 to 605 nm, and achieved a broad color temperature range (1996-9803 K). This research enhances the development of high-performance light sources toward lighting and indoor/underwater visible light communication.
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npj Science of Food
·
2026
·
IF 9.7
Zhang Huifen,Pu Jing,Wang Miao,Du Ying,Tang Xiaofu,Niu Jie,Wang Hongxia,Luo Xian,Wang Yongqing,Deng Qunxian
Loquat is highly susceptible to postharvest deterioration, including browning and lignification, which limits its storage life. This study investigated the effects of combined L-cysteine (L-Cys) and γ-aminobutyric acid (GABA) treatment on postharvest quality maintenance of loquat fruit and elucidated the underlying molecular mechanisms through integrated transcriptomic and metabolomic analyses. Physiological assessments revealed that the combined treatment significantly delayed fruit softening, reduced weight loss and browning, suppressed decay incidence, and maintained higher levels of soluble solids, titratable acidity, and total phenolics. Integrated multi-omics analysis demonstrated that L-Cys+GABA treatment primarily modulated the phenylpropanoid metabolic pathway, leading to suppressed lignin biosynthesis through coordinated downregulation of key biosynthetic genes ( COMT , CCR , LAC ) and reduced accumulation of corresponding metabolites. These findings reveal that L-Cys+GABA combined treatment delays postharvest senescence in loquat fruit by targeting the phenylpropanoid pathway to inhibit lignification, providing a physiological and molecular basis for its application as an effective preservation strategy.
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