FOOD CHEMISTRY
·
2024
·
IF 8.5
Linlin Bian,Xue Ge,Senwei Feng,Guangxuan Chen,Kefeng Li,Xu Wang
We developed a method for the determination of chloropropanol esters and glycidyl esters (GE) in instant noodles using solid-phase microextraction with chitosan-β-cyclodextrin (CS-β-CD) coated fiber coupled with gas chromatography-tandem mass spectrometry. The developed low-cost fiber coating can improve the sensitivity of the method. Immobilized enzymes can improve operational stability and reusability compared to free enzymes, thereby reducing costs. The adsorption isotherm was modeled using the Langmuir model, while the adsorption kinetics followed the pseudo second-order model. The limit of detection was 0.3 ng/L. The method exhibited satisfactory recoveries for the analytes, ranging from 80.2 % to 105.3 %, with relative standard deviations < 9.9 %. Furthermore, the results of the exposure assessment showed that chloropropanol esters do not pose unacceptable risks to different age groups. However, the margin of exposure for GE suggested a potential health risk for populations between the ages of 3 and 12 years old.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Shu Yang,Xueping Zhang,Ahmed S.M. Saleh,Lishuang Wang,Yumin Duan,Zhigang Xiao
Edible oil-based oleogels provide a viable and healthier alternative to traditional saturated fats. This study aimed to develop oleogels using rice bran oil combined with β-Sitosterol (Sit) and Palmitic Acid (PA). Structural, physicochemical, and rheological properties of the prepared oleogels were investigated. The results showed that Sit alone was insufficient to create a stable oleogel. However, when Sit was combined with PA, a firm and stable oleogel was obtained. The gel network was formed and stabilized through physical non-covalent interactions such as hydrogen bonding. Additionally, x-ray diffraction analysis indicated that the oleogel molecules rearranged and underwent a homogeneous polycrystalline transition due to the different gelator combinations, revealing the coexistence of β and β′ crystal types in the oleogels. Moreover, the firmness and oil binding capacity of the oleogels increased with higher PA content in the gelator mixture. Furthermore, the storage modulus (G′) and loss modulus (G″) were frequency-independent, with G′ significantly higher than G". The results suggest that fat-like oleogel can be produced using a combination of Sit and PA at mass ratios of Sit2:PA3 or Sit1:PA4 (w/w).
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Foods
·
2025
·Vol. 14
·期 7
·1108
·
IF 5.1
Jiaofen Lin,Jian Zeng,Guozong Shi,Zesheng Zhuo,Yanyun Guan,Zhipeng Li,Hui Ni,Peng Fei,Bingqing Huang
In this study, we developed a soybean oil-based emulsion system stabilized by caffeic acid-modified mushroom-derived chitosan, significantly enhancing its functional properties. The modification increased the grafting ratio from 5.02% to 8.26%, which greatly improved antioxidant activity and antimicrobial efficacy against Escherichia coli and Staphylococcus aureus. The modified chitosan exhibited superior rheological properties, including increased viscosity and elasticity, contributing to improved emulsification performance. Emulsions stabilized with caffeic acid-modified chitosan showed smaller and more uniform droplet sizes, along with greater stability, as indicated by a higher zeta potential (55.63 mV). These modifications resulted in enhanced β-carotene encapsulation efficiency (up to 87.46%) and improved bioaccessibility (up to 52.13%), highlighting the system’s potential as an efficient food-grade carrier for hydrophobic bioactive compounds. In conclusion, caffeic acid-modified mushroom chitosan is an effective natural emulsifier, enhancing stability, antioxidant activity, and nutrient delivery, and has promising applications in functional foods and nutraceuticals.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Minghui Cai,Haixu Yao,Mengnan Li,Yan Yan,Yang Cao,Jiaxin Li,Huiyu Su,Wenwen Zhang,Hui Zhu,Feiyun Cui
β-estradiol is one of the most active hormones in steroids, and it plays a vital role in the human reproductive and non-reproductive systems. Compared with other methods for detecting β-estradiol, electrochemical biosensors are economical, simple, sensitive, and rapid. Herein, spinel copper ferrite nanoparticles and laser-induced graphene (LIG) were employed to functionalize screen-printed carbon electrodes (SPCE) for the first time, and it demonstrated that they have a synergistic effect on the catalysis of β-estradiol. Differential pulse voltammetry (DPV) was used to detect the changes in the peak current generated by the oxidation of β-estradiol. The nanoparticles were characterized by Fourier infrared spectroscopy, transmission electron microscope, scanning electron microscopy, X-ray photoelectron spectroscopy (XPS), and X-ray Diffraction (XRD), and the composition elements and morphology of the nanoparticles were explained. The linear range of β-estradiol detected by this method is 0.1 ∼ 100 μM, R 2 = 0.9992, and the detection limit is 3 nM. The sensor has good selectivity, reproducibility, repeatability, stability, and anti-interference performance. In rat serum, the recovery of the sensor at 100, 10, and 1 μM β-estradiol was 105 % ∼ 110 %, with relative standard deviations of 2.3 % ∼ 4.0 % (N = 3). This study shows that the sensor can be applied to detect actual samples and has potential application value for female reproductive-related diseases.
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Food Biophysics
·
2024
·Vol. 20
·期 1
·1-13
·
IF 2.8
Chen Xing-Shuo,Yang Jie,Mao Yi-Nuo,Sun Gui-Jin,Li Ying-Qiu,Wang Chen-Ying,Liang Yan,Zhao Xiang-Zhong,Mo Hai-Zhen
This study investigated the bitterness, structure, physicochemical properties and functional properties of peanut protein hydrolysate (PPH) debittered using β-CD and 2-butanol. The sensory properties of debittered PPH were characterized using sensory evaluation combined with electronic tongue and colorimetric analysis. High-performance size exclusion chromatography (HPSEC) and Fourier transform infrared (FTIR) spectroscopy characterized the structures of debittered PPH. Surface hydrophobicity, components of amino acids, and DPPH and ABTS radical scavenging capacities characterized the functional and physicochemical properties of debittered PPH. Bitterness of PPHF-4 with combined treatment of β-CD and 2-butanol was negligible with bitterness score (0.24) and almost unchanged color. Moreover, PPHF-4 retained a high level of umami with the electronic tongue umami response value (12.95). Surface hydrophobicity and amino acid analyses showed that PPHF-4 had the lowest surface hydrophobicity (8.36) and hydrophobic amino acids (202.62 mg/g) content among PPHs debittered. HPSEC analysis showed that compared to untreated PPH, protein hydrolysates less than 5.57 kDa in PPHF-4 were obviously reduced and less than 1.68 kDa were completely removed. The DPPH and ABTS radical scavenging IC50 of PPHF-4 was 0.67 and 0.40 mg/mL, which was decreased but still had a high level of antioxidant properties compared to PPH (0.44 and 0.34 mg/mL).
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Molecular Catalysis
·
2024
·
IF 3.9
Qiqi Xing,Xiaoyu Wang,Qi Li,Changning Lu,Yi Wu,Jianjun Pei,Linguo Zhao
β-Galactosidase is a crucial class of biocatalysts, renowned for its ability to catalyze the hydrolysis of non-reducing terminal β-d-galactoside bonds, while also demonstrating significant potential in galactosylation synthesis reactions. In this study, a novel GH1 β-galactosidase gene VthBga was screened and cloned from Vulcanisaeta thermophila . Soluble expression of the VthBga was induced at 47 °C in Escherichia coli BL21. VthBga exhibited optimal activity at 95 °C and pH 4.5 with p -nitrophenyl-β-d-galactoside ( p NPGal) as a substrate and displayed excellent thermotolerance below 90 °C. In addition, VthBga possessed not only a diverse array of substrate hydrolytic activities but also inherent galactosylation activity, rendering it a promising candidate for the galactosylation of the natural compound. To enhance its transglycosylation activity, a superior mutant Vth-A266G/F441T was obtained by adjusting the transglycosylation/hydrolysis ratio through a semi-rational design. Under optimal conditions, this mutant produced 44.5 % tyrosol galactoside, representing a significant 2.3-fold increase compared to the wild-type strain, highlighting its practical value in related biosynthesis applications.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2017
·
IF 2.714
Jianxin Chen,Yunping Pu,Chongbin Wang,Jian Han,Yunlong Zhong,Kaili Liu
β-cyclodextrin, a type of molecule with a cavity structure and large number of hydroxyl groups , has great potential in water treatment, attributing to its highly adsorption ability. In this work, silica nanoparticles coated with β-cyclodextrin (SiO 2 @β-CD) as adsorbent have been successfully synthesized via distillation-precipitation polymerization. The composition , morphology and structure of SiO 2 @β-CD nanoparticles were characterized by FTIR and TEM . The adsorption performance of SiO 2 @β-CD nanoparticles were analyzed by the adsorption ability of bromophenol blue (BPB) and crystal violet (CV) in water solution. In addition, the dyes sorption processes have been thoroughly studied with both kinetic and equilibrium models. The results show that the adsorption rates for dyes is fast and the time evolution can be perfectly fitted using a pseudo-second-order kinetic model. The obtained maximum adsorption capacities exhibit an extremely good ability to remove dye at ambient temperature.
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FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Qingbo Jiao,Gerui Ren,Nan Lv,Hao Li,Tianrong Wang,Min Huang,Ruibo Wu,Zexing Cao,Hujun Xie
Enhancing protein-protein interactions (PPIs) has been intensively studied, and the use of molecular glues is a cutting-edge technology. Although the number of molecular glues approved by the Food and Drug Administration is limited, (−)-Epigallocatechin-3-gallate (EGCG) has shown potential as a molecular glue. This study aimed to investigate how EGCG regulated the mechanism of PPIs using zein and β-lactoglobulin (β-LG) as model proteins. The molecular dynamics simulations revealed that EGCG enhanced hydrogen bonding and hydrophobic interactions in Zein-β-LG complexes, thereby improving their stability and compactness. The Δ G values for the formation of the complexes were all higher than −20 kcal/mol, suggesting strong protein interactions and spontaneous binding process. To validate the theoretical calculations, experimental verification was also conducted. The turbidity, particle size, and ζ-potential results demonstrated that EGCG enhanced the aggregation and stability of Zein-β-LG complexes. Fluorescence experiments indicated that the Δ G values at 298K were −27.57 kJ‧mol −1 at pH 5.5, −26.26 kJ‧mol −1 at pH 7.0, and −25.58 kJ‧mol −1 at pH 2.5, and the corresponding binding sites were 1.036, 1.018, and 1.118, respectively. Thermodynamic parameters suggested that hydrogen bonding and hydrophobic interactions were the main driving forces involved in the complexation of EGCG and Zein-β-LG. EGCG binding significantly altered the secondary structures and surface properties of the complexes. These insights emphasized the potential of EGCG in modulating protein interactions, with significant implications for protein-based material and delivery system in the food and pharmaceutical industries.
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Analytical Methods
·
2024
·Vol. 16
·期 25
·4104-4115
·
IF 2.7
Hong Cui,Qiuju Tian,Yunfeng Liu,Liangpo Liu,Xiangjun Li,Jisheng Nie,Ruiping Zhang
Fluoroquinolone (FQ) antibiotics, one of the leading environmental pollutants, have ecotoxic effects that can accumulate through ecosystems and harm human health. The determination of FQs is still difficult due to the complex matrix, many interfering factors, and low concentration. Hence, a magnetic microporous organic network (MON) composite denoted as Fe3O4@MON-NH2@CM-β-CD with excellent FQ adsorption performance was prepared by β-CD covalent modification of a MON. Based on the existence of π–π packing, hydrophobic interaction, and hydrogen bonding between Fe3O4@MON-NH2@CM-β-CD and FQs, a new magnetic solid phase extraction (MSPE) method for the enrichment of FQs was developed. Under optimized MSPE conditions, five FQs were detected by HPLC-UV with good linearity (R2 ≥ 0.9989) in the range of 0.02–1 μg mL−1, and detection limits (S/N = 3) in the range of 0.0014–0.0023 μg mL−1. The satisfactory recoveries ranged from 93.1 to 116.2% with RSDs lower than 8.39% when applied to actual environmental water samples. These results revealed that Fe3O4@MON-NH2@CM-β-CD as an adsorbent for MSPE had excellent performance for FQ extraction from real samples, and the MON material types were expanded through the functionalization of MONs, which would have great potential for further application in various analytical methods.
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DIAMOND AND RELATED MATERIALS
·
2024
·
IF 4.3
YuXuan Xiong,Dandan Luo,Guowen He,Saiwen Liu,Jin Zhang,Chao Chen
Baicalin is a principal component of traditional Chinese medicine and plays a crucial role in treating conditions such as arthritis, respiratory tract infections, chronic and acute hepatitis, and tumors. Graphene oxide is bonded to silicon dioxide (SiO 2 ) through covalent bonds or hydrogen bonds and then reduced to SiO 2 @rGO through hydrazine hydrate to solve the problem of easy accumulation of SiO 2 and improve the conductivity of the material. Next, β-cyclodextrin (β-CD) self-assembles on SiO 2 @rGO to form a stable inclusion complex. The hydrophobic inner cavity of β-CD can effectively accommodate various guest molecules to form inclusion complexes, enhancing molecular recognition and detection capabilities. Finally, β-CD/SiO 2 @rGO is bridged and surface-loaded between clay-like Ti 3 C 2 T x (MXenes) nanosheets through electrostatic interactions to form a stable three-dimensional composite material. It is precisely because of MXenes, due to their unique layered microstructure, excellent conductivity, and extensive hydrophilic surface area, that they are conducive to self-assembly or embedding nanoparticles in aqueous solution. The composite was characterized via X-ray diffraction, X-ray photoelectron spectroscopy, and scanning electron microscopy. Under optimized conditions, the electrochemical sensor constructed from this composite exhibited a broad detection range (0.008–10 μM) and a low detection limit (0.191 nM) for baicalin. In comparison to existing methods for detecting baicalin, this approach demonstrates strong anti-interference capabilities and stability. It can efficiently, sensitively, and accurately quantify baicalin in Chinese patent medicine samples, including Scutellaria baicalensis root, Shuanghuanglian oral liquid, and Bear Bile Scutellaria baicalensis eye drops, achieving a satisfactory recovery rate. This study introduces a novel approach for the electrochemical detection of baicalin.
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Journal of Functional Foods
·
2024
·
IF 3.8
Xilin Jiang,Jian Tang,Zhiqiang Gao,Caixia Chen,Wenjun Li,Lu Peng,Hongwei Li,Hongying Zhao,Yun Zhao
Moringa oleifera Lam. ( M. oleifera ) has been used as a remedy for diabetes clinically. To discover the underlying mechanism, we investigated its hypoglycemic compounds and shed light on its regulatory targets and pathways using network pharmacology. A total of 13 active compounds of stem were shot, and they may relieve diabetes through 122 core intersecting targets, mainly regulating the biological processes of inflammatory response and apoptosis. Molecular docking showed binding advantages of the active compounds with proteins in apoptosis process. In experiments conducted on MIN6 cells, an islet β-cell line, the extract of M. oleifera stem was validated to protect cells from apoptosis through reducing cellular ROS and endoplasmic reticulum stress. And consistently with its protective effect on islet β-cells, M. oleifera stem alleviated hyperglycemia in Type 2 diabetes mellitus (T2DM) mice. These results offer novel evidence for the development of functional foods to treat T2DM with M. oleifera stem.
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Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Changzhe Zhou,Haojie Li,Longlong Wang,Tao Fu,Guangli Liu,Xiaoran Shen,Huazhang Zhao
Polyester membranes are increasingly utilized in wastewater treatment due to their chlorine resistance. Nonetheless, they face challenges such as limited permeability. The integration of covalent organic frameworks (COFs) can significantly enhance the performance of nanofiltration membranes. However, the heterogeneous dispersion of COFs in solution results in poor compatibility with polyester membranes, leading to increased membrane defects and compromising nanofiltration performance. This study presents the development of COFs-polyester hybrid membranes via a simple one-step in-situ co-polymerization method. During the interfacial polymerization (IP), p-phenylenediamine (Pa) and 1,3,5-triformylphloroglucinol (Tp) were integrated into a β-cyclodextrin (β-CD) aqueous solution and a trimesoyl chloride (TMC) organic solution, respectively. The uniform dispersion of COFs monomers within the IP monomer solution addressed heterogeneity issues, improving compatibility with polyester membranes. The in-situ co-polymerized COFs not only enhanced the sieving capabilities but also improved the hydrophilicity, and free volume of the membranes. The optimized membranes demonstrated a permeance of 105 L/m 2 ·h·bar, with rejection rates of 98.5 % for Congo red and 6 % for Na 2 SO 4 . Moreover, these membranes exhibited remarkable resistance to chlorine, organic solvents, and antifouling capabilities. This study offers novel insights into the application of COFs in polyester membranes, providing a straightforward, cost-effective, and scalable integration strategy.
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