ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2022
·Vol. 29
·期 24
·36216-36224
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IF 5.19
Guo Zhao-Bing,Sun Wen-Long,Zuo Xiao-Jun,Song Hai-Liang,Ling Hao,Zhang Shuai
Microbial electrolysis cells (MECs) have been applied for antibiotic degradation but simultaneously induced antibiotic resistance genes (ARGs), thus representing a risk to disseminate antibiotic resistance. However, few studies were on the potential and risk of ARGs transmission in the MECs. This work assessed conjugative transfer of ARGs under three tested conditions (voltages, cell concentration, and donor/recipient ratio) in both single- and two-chamber MECs. The results indicated that voltages (> 0.9 V) facilitated the horizontal frequency of ARGs in the single-chamber MECs and anode chamber of two-chamber MECs. The donor cell number (donor/recipient ratio was 2:1) increased the transfer frequency of ARGs. Furthermore, voltages ranged from 0.9 to 2.5 V increased reactive oxygen species (ROS) production and cell membrane permeability in MECs. These findings offer new insights into the roles of ARG transfer under different applied voltages in the MECs, which should not be ignored for horizontal transfer of antibiotic resistance.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2022
·Vol. 20
·期 1
·1-12
·
IF 9.429
Duan Wenyao,Li Bo,Zhang Wen,Li Jiaqi,Yao Xin,Tian Yupeng,Zheng Jun,Li Dandan
Designing new oxygenation nanomaterials by oxygen-generating or oxygen-carrying strategies in hypoxia-associated anti-tumor therapy is a high priority target yet challenge. In this work, we fabricated a nanoplatform involving Fenton-like reaction, Pd@MOF-525@HA, to relieve tumor hypoxia via oxygen-generating strategy for enhanced oxygen-dependent anti-tumor therapy. Thereinto, the porphyrinic MOF-525 can produce singlet oxygen (1O2) via light or ultrasonic irradiation for photodynamic and sonodynamic therapy. Notably, the well-dispersed Pd nanocubes within MOF-525 can convert H2O2 into O2 to mitigate the hypoxic environment for enhanced therapy outcome. Moreover, the two-photon activity and cancer cell specific targeting capability of Pd@MOF-525@HA gave rise to deeper tissue penetration and near-infrared light-induced fluorescence imaging to achieve precise guidance for cancer therapy. This work provides a feasible way in designing new oxygenation nanomaterials to relieve tumor hypoxia for enhanced cancer treatment.Graphical Abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 17
·4837-4847
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IF 4.478
Lv Xiaoyi,Hu Qiong,Miao Tian,Li YanPing,Cui Bo,Fang Yishan
Herein, we fabricated a label-free ECL immunosensor for aflatoxin B 1 (AFB 1 ) detection. In this system, a small organic aggregation-induced electrochemiluminescence luminophore, 2,5-di-tetraphenylethylene-ylthiazolo [5,4-d] thiazole, was designed, named TPETTZ. Polyaniline-wrapped TiO 2 nanoparticles (PANI/TiO 2 NPs) complex was synthesized through one-step in situ oxidation polymerization of aniline, and performed excellent electrical conductivity and abundant amino groups. As an ECL accelerator, TiO 2 nanoparticles (TiO 2 NPs) promoted the oxidation of tri- n -propylamine (TPA) to generate more TPA • ; in addition, it also acted as a donor to improve the ECL intensity of TPETTZ (acceptor) through electrochemiluminescence resonance energy transfer (ECL-RET). Encouraged by the above, under the existence of TPA, TPETTZ displayed a strong and continuously stable ECL anode signal due to the introduction of PANI/TiO 2 NPs. Therefore, the immunosensor was constructed for AFB 1 detection based on the quenching effect of target on the ECL signal, and a linearly decreasing ECL signal was obtained as the increasement of AFB 1 in the range of 75 fg/mL to 100 ng/mL, with a lower detection limit of 27.5 fg/mL. Moreover, the as-prepared sensing platform performed a satisfactory anti-interference, stability, and reproducibility, and appeared a good accuracy in walnut sample analysis, presenting a promising application in the future.
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NPG Asia Materials
·
2022
·Vol. 14
·期 1
·1-10
·
IF 10.761
Wen Guo-Yu,Zhou Xing-Long,Tian Xiao-Yu,Xie Rui,Ju Xiao-Jie,Liu Zhuang,Faraj Yousef,Wang Wei,Chu Liang-Yin
Pigmentary coloration can produce viewing angle-independent uniform colors via light absorption by chromophores. However, due to the limited diversity in the changes of the molecular configuration of chromophores to undergo color change, the existing materials cannot produce a wide range of visible colors with tunable color saturation and transmittance. Herein, we propose a novel strategy to create materials with a wide visible color range and highly tunable color saturation and transmittance. We fabricated a hydrogel with poly (acrylamide- co -dopamine acrylamide) networks swollen with Fe 3+ -containing glycerol/water in which the covalently crosslinked polyacrylamide backbone with pendant catechols can ensure that the hydrogel maintains a very stable shape. Hydrogels containing adjustable catechol-Fe 3+ coordination bonds with flexible light-interacting configuration changes can display a wide range of visible colors based on the complementary color principle. The catechol-Fe 3+ complexes can dynamically switch between noncoordinated and mono-, bis- and tris-coordinated states to harvest light energy from a specific wavelength across the whole visible spectrum. Therefore, these hydrogels can be yellow, green, blue, and red, covering the three primary colors. Moreover, color saturation and transmittance can be flexibly manipulated by simply adjusting the Fe 3+ content in the hydrogel networks. The versatility of these smart hydrogels has been demonstrated through diverse applications, including optical filters for color regulation and colorimetric sensors for detecting UV light and chemical vapors. This proposed smart hydrogel provides a universal color-switchable platform for the development of multifunctional optical systems such as optical filters, sensors, and detectors.
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IONICS
·
2022
·Vol. 28
·期 6
·2931-2942
·
IF 2.961
Zhu Shiling,Chen Susu,Zhang Hao,Yu Wenxing,Tian Yuanhao,Hu Xiaolin,Hussain Shahid,Lin Liyang,Chen Wei,Xu Chaohe
Vanadium pentoxide (V 2 O 5 ), as a common supercapacitor electrode material, has unsatisfactory electrochemical performance due to its low conductivity and ionic diffusion rate, especially its specific capacitance and rate capability, which limits its practical use. In this work, V 2 O 5 rich in oxygen vacancies is successfully prepared by a controllable two-step reduction method. A variety of advanced characterization techniques, especially X-ray photoelectron spectroscopy (XPS) and electron paramagnetic resonance (EPR) spectra, have been used to qualitatively monitor changes in oxygen vacancy content. Compared with the untreated hydrated V 2 O 5 nanosheets, the oxygen vacancy V 2 O 5 (Vö V 2 O 5 ) has a larger specific surface area (4.002 m 2 g −1 ); besides, its specific capacitance (619.5 F g −1 in 1 A g −1 ) being not only better than that of V 2 O 5 without oxygen vacancy treatment (308.0 F g −1 in 1 A g −1 ) but also its cycle stability being improved (70.7% capacity retention after 1000 cycles), it is possible to develop high-performance supercapacitors by using it as an electrode material.
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Molecular Cancer
·
2022
·Vol. 21
·期 1
·1-17
·
IF 41.444
Pan Zhenzhen,Wang Kai,Wang Xiniao,Jia Zhirong,Yang Yuqi,Duan Yalei,Huang Lianzhan,Wu Zhuo-Xun,Zhang Jian-ye,Ding Xuansheng
Background The use of epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) brings remarkable benefits for the survival of patients with advanced NSCLC harboring EGFR mutations. Unfortunately, acquired resistance seems to be inevitable and limits the application of EGFR-TKIs in clinical practice. This study reported a common molecular mechanism sustaining resistance and potential treatment options to overcome EGFR-TKIs resistance.Methods EGFR-TKIs resistant NSCLC cells were established and confirmed by MTT assay. Cholesterol content was detected and the promotional function of cholesterol on NSCLC growth was determined in vivo. Then, we identified ERRα expression as the downstream factor of cholesterol-mediated drug resistance. To dissect the regulatory mechanism, we conducted experiments, including immunofluorescence, co-immunoprecipitation, luciferase reporter assay and chromatin immunoprecipitation assay.Results Long-term exposure to EGFR-TKIs generate drug resistance with the characteristic of cholesterol accumulation in lipid rafts, which promotes EGFR and Src to interact and lead EGFR/Src/Erk signaling reactivation-mediated SP1 nuclear translocation and ERRα re-expression. Further investigation identifies ERRα as a target gene of SP1. Functionally, re-expression of ERRα sustains cell proliferation by regulating ROS detoxification process. Lovastatin, a drug used to decrease cholesterol level, and XCT790, an inverse agonist of ERRα, overcome gefitinib and osimertinib resistance both in vitro and in vivo.Conclusion sOur study indicates that cholesterol/EGFR/Src/Erk/SP1 axis-induced ERRα re-expression promotes survival of gefitinib and osimertinib-resistant cancer cells. Besides, we demonstrate the potential of lowing cholesterol and downregulation of ERRα as effective adjuvant treatment of NSCLC.
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APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
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2022
·Vol. 128
·期 4
·1-8
·
IF 2.983
Xu Tianwen,Wang Xueqin,Zhang Xin,Bai Zhongchen
An ideal surface-enhanced Raman scattering (SERS) substrate should exhibit a high electromagnetic field enhancement factor, simple preparation repeatability low cost. Here, we prepared a reproducible nanoplasmonic paper SERS substrate via a silver mirror reaction (SMR) and AgNO 3 -ascorbic acid (AgNO 3 -ASC) solution treatment method to reduce Ag nanoparticles (Ag NPs) on the surface of the flexible glass fiber filter paper (GFP). GFP with Ag NPs (AgNPs@GFP) was used for SERS to detect melamine molecules (MMs). The results showed that melamine molecules’ detection limit was up to 1 ng/mL. The maximum enhancement factor of the SERS signal reached 838, and the sensitivity was 150. This strategy of fabricating SERS substrates based on GFP can provide a new way to prepare a low-cost flexible SERS substrate and increase the sensitivity of biomolecules.
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RARE METALS
·
2022
·Vol. 41
·期 7
·2292-2304
·
IF 6.318
Zhang Le-Xi,Zhao Ming-Mei,Yin Yan-Yan,Xing Yue,Bie Li-Jian
Comprehensive consideration of structural and electronic sensitization is of significant importance for rational design and assembly of high-performance gas sensors based on metal oxides. In this work, hierarchically mesoporous ZnO nanosheets are synthesized via a hydrothermal method followed by calcination. Material characterization reveals polycrystalline feature of these ZnO nanosheets rich in mesopores and defects. Gas sensing performance of as-synthesized ZnO nanosheets was systematically investigated, taking formaldehyde as probe molecules. Compared with commercial ZnO nanoparticles, ZnO nanosheets exhibited enhanced formaldehyde sensing properties, including lower operation temperature, higher sensitivity, faster response, and smaller detection limit. Notably, the response ( S = 227.4) of ZnO nanosheets to 200 × 10 –6 formaldehyde is about 17 times larger than that of ZnO nanoparticles ( S = 13.5). Excluding effects of grain size and surface area, enhanced sensing properties, especially response value, are credited in large part to synergistic actions of surface defects, grain boundaries, as well as unique structural advantage of ZnO nanosheets. Furthermore, this work offers a guideline for boosting performance of metal oxide-based gas sensors via surface defect control and grain boundary construction. Graphical abstract
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Microbial Cell Factories
·
2022
·Vol. 21
·期 1
·1-12
·
IF 6.352
Yang Fengyu,Xu Jiayu,Zhu Yichun,Wang Yi,Xu Meijuan,Rao Zhiming
Background Agmatine is a member of biogenic amines and is an important medicine which is widely used to regulate body balance and neuroprotective effects. At present, the industrial production of agmatine mainly depends on the chemical method, but it is often accompanied by problems including cumbersome processes, harsh reaction conditions, toxic substances production and heavy environmental pollution. Therefore, to tackle the above issues, arginine decarboxylase was overexpressed heterologously and rationally designed in Corynebacterium crenatum to produce agmatine from glucose by one-step fermentation.Results In this study, we report the development in the Generally Regarded as Safe (GRAS) l-arginine-overproducing C. crenatum for high-titer agmatine biosynthesis through overexpressing arginine decarboxylase based on metabolic engineering. Then, arginine decarboxylase was mutated to release feedback inhibition and improve catalytic activity. Subsequently, the specific enzyme activity and half-inhibitory concentration of I534D mutant were increased 35.7% and 48.1%, respectively. The agmatine production of the whole-cell bioconversion with AGM3 was increased by 19.3% than the AGM2. Finally, 45.26 g/L agmatine with the yield of 0.31 g/g glucose was achieved by one-step fermentation of the engineered C. crenatum with overexpression of speAI534D.Conclusion sThe engineered C. crenatum strain AGM3 in this work was proved as an efficient microbial cell factory for the industrial fermentative production of agmatine. Based on the insights from this work, further producing other valuable biochemicals derived from l-arginine by Corynebacterium crenatum is feasible.
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Reaction Kinetics Mechanisms and Catalysis
·
2022
·Vol. 135
·期 2
·795-810
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IF 1.843
Liu Panli,Shi Shengbin,Gao Lijing,Xiao Guomin
In the present work, three immobilized imidazolium acidic ionic liquid catalysts (ImmHSO 4 -IL, ImmCH 3 SO 3 -IL and ImmH 2 PO 4 -IL) were prepared with silica as carrier and modified with H 2 SO 4 , CH 3 SO 3 H and H 3 PO 4 , which were used as catalysts in the production of furfural from xylan and rice husk. The characteristics of the synthesized catalysts were analyzed by various characterization techniques, and the effects of reaction conditions, including reaction solvent, reaction time, reaction temperature and the amount of catalyst on the yield of furfural were studied in detail. The results showed that the highest yield of furfural reached 72% and 74.6% when using xylan and rice husk as substrates, respectively, which is one of the best furfural yield among literature reports. In addition, the ImmHSO 4 -IL catalyst has excellent selectivity for hemicellulose in rice husk, and the catalyst has high catalytic activity after four cycles. Therefore, the application of ImmHSO 4 -IL catalyst provides a new approach for conversion biomass to furfural. Graphical abstract
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 40
·61534-61546
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IF 5.19
Tan Jiangtao,Chen Yongjian,Mo Zhaowen,Tan Chunju,Wen Runhao,Chen Zhengtong,Tian Hua
Metal nanoparticles and microplastics are becoming important pollutants in agricultural fields, but there are few studies on the interaction of zinc oxide nanoparticles (ZnONPs) and polyethylene (PE) microplastics with rice seedlings. The two rice cultivars Xiangyaxiangzhan and Yuxiangyouzhan were grown at three ZnONP levels (0 mg L −1 , 50 mg L −1 , and 500 mg L −1 ) and three PE levels (0 mg L −1 , 250 mg L −1 , and 500 mg L −1 ), and the growth, physiological attributes, and Zn uptake of rice seedlings were measured. Result showed that the ZnONPs and PE treatment effects on the investigated parameters differed between the cultivars, whilst Yuxiangyouzhan produced 6.98% higher in mean total dry biomass than Xiangyaxiangzhan. The mean total dry biomass in Xiangyaxiagnzhan and Yuxiangyouzhan changed by 10.22–30.85% and − 11.74–25.58% under ZnONPs, respectively. The PE treatments reduced growth parameters in Xiangyaxiangzhan, whilst the 250 mg L −1 PE treatment reduced the growth parameter of Yuxiangyouzhan. Besides, the ZnONP treatment had a stronger effect on rice seedling growth than the PE treatment. Furthermore, the ZnONPs modulated the physiological parameter in plant tissue of the two rice varieties. ZnONP treatment lead to the accumulation of Zn in plant tissue and the shoot Zn content was strongly related to shoot cellulose content. Overall, ZnONPs and PE treatments modulated the growth, physiological and biochemical attributes, and Zn uptake of rice seedlings, and the cultivars and dose effects could not be ignored.
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JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 16
·7896-7908
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IF 4.682
Xu Tianye,Lu Shun,Wang Yajie,Gan Xiaoyan,Guo Liling,Liu Hanxing
Two-dimensional (2D) perovskite formed by introducing various organic spacer cations into 3D perovskite is considered to be one of the most effective methods to improve the humidity stability of perovskite materials. In order to solve the phase inhomogeneity and phase distribution nonuniformity of two-dimensional perovskites, additive engineering technique is widely used during their fabrication processes. Although many additives are proved to be effective in improving the quality of perovskite films, the mechanism of additive engineering has not been fully understood. In our work, we prepare 2D Ruddlesden–Popper perovskites based on 4-bromophenylethylammonium (BPEA) using a new additive of ionic liquids MAAc (methylammonium acetate). The (BPEA) 2 (MA) 4 Pb 5 I 16 perovskite prepared with the assistance of MAAc exhibits a stable photoelectric conversion efficiency (PCE) of 8.47%, which is much higher than the one without MAAc additive (4.79%). Besides a nearly tenfold increase in electron mobility, the unencapsulated (BPEA) 2 (MA) 4 Pb 5 I 16 -based device with MAAc additive remains 88% of its initial efficiency after 960 h exposure to air with a humidity of 20 ± 5% at room temperature, while the device without additive decreases to 63% of its initial efficiency. Meanwhile, the interactions between PbI 2 and MAAc at different temperatures and molar ratios are investigated and the formation mechanism of 2D perovskites in the presence of MAAc is proposed. It is found that an intermediate perovskite with distorted [PbI 6− x Ac x ] 4− octahedron is formed with the addition of MAAc, making the quality and photoelectric properties of the resultant films greatly enhanced.
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