Nature Communications
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2025
·Vol. 16
·期 1
·1-20
·
IF 15.7
Zhang Jiayu,Yang Jinneng,Luo Jinyan,Wu Weili,Luo Haidan,Wei Wenxia,Lyu Haimei,Wang Yuzhi,Yi Hairong,Zhang Yijing,Fan Zongmin,Lyu Haiwen,Kanakaveti Vishnu Priya,Qin Baifu,Yuan Ping,Yang Runxiang,Zhang Haipeng,Zuo Tao,Felsher Dean W.,Lee Mong-Hong,Li Kai
Oncolytic viruses (OVs) hold promise for cancer treatment. However, the antitumor efficacy is limited. Microbiota plays a pivotal role in cancer treatment and its impact on oncolytic virotherapy is unknown. Here, we show that VSVΔ51 has higher antitumor efficacy for hepatocellular carcinoma in the absence of microbiota in female mouse models. VSVΔ51 infection causes microbiota dysbiosis, increasing most of the gut bacteria abundance, while decreasing the commensal Lactobacillus . VSVΔ51 reduced intestinal expression of SLC20A1 that binds to Lactobacillus acidophilus ( L. acidophilus ) CdpA cell wall protein through IL6-JAK-STAT3 signaling, thereby attenuating attachment and colonization of L. acidophilus . L. acidophilus supplementation confers sensitivity to VSVΔ51 through restoring gut barrier integrity and microbiota homeostasis destroyed by VSVΔ51. In this work, we show that targeting microbiota homostasis holds substantial potential in improving therapeutic outcomes of oncolytic virotherapy.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-19
·
IF 15.7
Peng Han,Xu Qikui,Zhang Ting,Zhu Jiakai,Pan Jinheng,Guan Xiaoyu,Feng Shan,Wu Jianping,Hu Qi
Lipid droplets (LDs) are the main cellular storage sites for triacylglycerols (TAGs), playing an important role in energy homeostasis and cell signaling. Hydrolysis of the stored TAGs begins with conversion of TAGs into diacylglycerols (DAGs) by adipose triglyceride lipase (ATGL), followed by hydrolysis of DAGs by hormone-sensitive lipase (HSL). Despite the central role of HSL in lipolysis, the molecular determinants for its LD association have remained elusive. Here, we report the cryo-EM structure of human HSL at 3.4 Å. Combining this with hydrogen-deuterium exchange mass spectrometry, biochemical and cellular assays, we identify residues 489-538, referred to as the “H-motif”, and the N-terminal 4-helix bundle of HSL as LD-binding motifs mediating direct interaction of HSL with LDs. LD binding mediated by the LD-binding motifs is independent of HSL phosphorylation catalyzed by the cAMP-dependent kinase PKA. Our findings provide insight into the LD binding mechanism of HSL, advancing our understanding of the regulation of lipolysis.
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International Journal of Environmental Science and Technology
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2024
·
IF 3
Cheng T.,Chen C.,Wen M.,Pan F.,Zhang X.,Ma H.,Hou B.,Xin X.
Developing highly efficient and low-cost composite electrode is a crucial step to achieve hydrogen and oxygen evolution reaction from overall water splitting. Herein, a ternary composite electrode derived from coating fly ash magnetic spheres and Graphite nanosheet on the carbon cloth surface, could greatly boost hydrogen and oxygen evolution reaction. Meanwhile, the efficiencies of overall water splitting were highly enhanced. The microstructure characterizations of composite material revealed that abundant magnetite crystals were existed on the fly ash magnetic spheres surface, and the main exposed crystal plane was active Fe 3 O 4 (111). Moreover, the composite electrode exhibited remarkable catalytic activities for hydrogen and oxygen evolution reaction, with lower overpotential of 175.6 mV and 328 mV at 10 mA·cm −2 , small Tafel slope of 45.5 mV·dec −1 and 77.9 mV·dec −1 , respectively, accompanied by prominent cycle stability. The overpotentials of hydrogen and oxygen evolution reaction were increased merely after 3000 CV cycles. Moreover, the electrocatalysis reaction mechanisms conducted by Density Functional Theory calculation illustrated that the combination of both advantages of Graphite nanosheet displaying superior electron transmission capacity, and the crystal plane of Fe 3 O 4 (111) gaining excellent electrocatalytic activity, gave ternary composite noticeably improved performances toward hydrogen and oxygen evolution reactions. Specifically, it required merely 1.73 V of potential difference to onset the overall water splitting reaction. This work opens a strategy to develop low-cost, efficient and earth-abundant catalysts for water splitting, and broaden the comprehensive utilization way of coal fired fly ash.
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Journal of Coatings Technology and Research
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2024
·
IF 2.3
Chen Lvxin,Yu Zhijie,Fang Yinchun
Structural colorization as a new green coloring method has received more and more attention. However, structural colors with only color can no longer meet the actual needs. It is of great practical significance to develop functional structural colors with brilliant colors and special function. In this study, Cu 2 O single crystal spheres with different sizes were successfully prepared and were sprayed on the surface of polyester/cotton fabric to obtain bright purple, purple red, light blue, dark green, grass green, and orange–yellow structural colors. Due to the introduction of polyacrylate (PA) adhesive, Cu 2 O structural colorized fabrics had excellent abrasion and washing colorfastness and stability. Meanwhile, the Cu 2 O structural color has little influence on the original softness and air permeability of fabric. The structural colorized fabric showed excellent antibacterial properties against E. coli and S. aureus , and the antibacterial rates decreased with the increasing of particle sizes of Cu 2 O spheres. The large-scale preparation of uniform structural colors on fabric can be realized by the spraying method. The fine and complex structural color patterns can also be obtained by spraying Cu 2 O single crystal spheres, showing good industrial application prospects. Therefore, this study provides experimental basis for the development of functional structural colors with brilliant colors and excellent antibacterial properties. Graphical abstract
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2024
·Vol. 35
·期 14
·1-13
·
IF 2.8
Hou Chunping,Yue Zeyu,Sun Hehang,Zhai Lidong,Xie Haidong,Tian Hui,Qu Yuqing,Wang Xinwei,Hou Jiao
Silicon oxide (SiO x ) anode materials have gained significant attention in lithium-ion batteries due to their high theoretical specific capacity (above 1965 mAh g −1 ), relatively stable cycling performance, and lower production costs. However, SiO x anode materials tend to form a solid electrolyte interphase (SEI) film and generate inert substances like Li 2 O and Li 4 SiO 4 during the initial charge and discharge processes, leading to the consumption of active lithium ions and low initial coulombic efficiency. To address these issues effectively, prelithiation of SiO x anode materials is explored in this study. A lithium powder suspension is prepared by dispersing lithium powder and carbon nanotubes (CNTs) in the electrolyte, served as the prelithiation agent. The SiO/G@C@A anode material is then prelithiated with this agent. The lithium powder suspension prelithiation agent enables effective prelithiation of the anode material. The SiO@C@A-1 anode exhibits improved electrochemical performances. At a current density of 0.1 A g −1 , the initial specific capacity reaches 981.3 mAh g −1 with an initial coulombic efficiency of 95.65%, which is approximately 25% higher than that of the untreated sample. After 100 cycles and at a current density of 0.5 A g −1 , the prelithiated sample retains a discharge specific capacity of 648 mAh g −1 , with a capacity retention rate of 83.27%, around 10% higher than that of the untreated sample, demonstrating excellent cycling performances.
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WATER AIR AND SOIL POLLUTION
·
2024
·Vol. 235
·期 8
·1-12
·
IF 3.8
Li Qi,Zhu Yanping,Liu Yanbiao,Chen Xiaoguang,Jiang Nan,Yan Mengying,Li Xuhua,Xing Haoyu,Bao Yinzhou,Huang Manhong
Antimonate (Sb(V)), sulfate and acid orange 7 (AO7) are common pollutants in printing and dyeing wastewater. Currently, there have been no reports about the effects of sulfate and AO7 on Sb(V) removal by anaerobic granular sludge (AnGS). In this study, the Sb(V) removal and Sb(Ш) accumulation was revealed in presence of sulfate and AO7, and the role of adding granular activated carbon (GAC) was investigated. Results showed that sulfate inhibited Sb(V) removal by AnGS (S + Sb, k = 0.101). GAC alleviated the inhibition of sulfate on Sb(V) removal and Sb(V) removal rate increased by 1.9 times (GAC + S + Sb, k = 0.187). Meanwhile, GAC promoted sulfate reduction by AnGS. AO7 inhibited sulfate reduction, thereby alleviating the inhibition of sulfate on Sb(V) removal, and Sb(V) removal rate increased by 1.5 times (S + Sb + dye, k = 0.147). When Sb(V), sulfate and AO7 coexisted, adding GAC actually inhibited Sb(V) removal (GAC + S + Sb + dye, k = 0.067), which may be due to GAC promoted the reduction of sulfate and AO7 by AnGS. The microbial community analysis revealed that Acinetobacter genus was related to Sb(V) reduction, with higher relative abundance in GAC + S + Sb (49.4%) and S + Sb + dye groups (28.9%). These results provided guidance for anaerobic treatment of printing and dyeing wastewater. Graphical Abstract
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Scientific Reports
·
2024
·Vol. 14
·期 1
·1-11
·
IF 3.8
Xie S. D.,Cai X. Y.,Wu H. B.,Wang Q.,Guo Z. R.,Chen Z. Y.,Ma C. S.
To study the effect of internal particle size on the microstructure properties and thermal decomposition characteristics of site mixed emulsion explosive at different altitudes. Site mixed emulsion explosive was prepared with different shear rate. The particle size, viscosity, sensitized bubbles, detonation velocity and peak pressure of the emulsion explosive were tested after stored at different simulated altitudes. The thermal decomposition characteristics of emulsion matrix prepared at three different rotational speeds were measured by thermogravimetric analyzer and kinetic analysis was performed by non-isothermal model Kissinger–Akah–Sunose (KAS) method. The results show that with the increase in altitude, the internal phase size showed a trend of first increasing and then decreasing, and the number of sensitized bubbles within the emulsion explosive decreases. At an altitude of 0 m, the detonation velocity and peak overpressure of the emulsion explosive prepared by 1600 r min −1 increased 4.78% and 29.09%, respectively compared with 1200 r min −1 , and at an altitude of 4500 m, the detonation velocity increased 11.87%, the peak overpressure increased 43.98%. The thermal decomposition activation energy of the emulsion matrix at 1600 r min −1 increased 13.14% compared to 1200 r min −1 . It shows that in the production of site mixed emulsion explosive at high altitude, reducing the particle size of the internal phase of emulsion explosives in a certain range can effectively improve the performance of emulsion explosives.
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Cell Death Discovery
·
2025
·Vol. 11
·期 1
·1-16
·
IF 7
Liang Xue,Tian Ruofei,Li Ting,Wang Hao,Qin Yifei,Qian Meirui,Fan Jing,Wang Dan,Cui Hong-Yong,Jiang Jianli
Pancreatic cancer exhibits high mortality rates with limited therapeutic options. Emerging evidence suggests that the ketogenic diet may act as adjuvant therapy by triggering ferroptosis in cancer cells, though the underlying molecular mechanisms remain unclear. This study aims to investigate the molecular mechanisms linking ketogenic metabolism and ferroptosis, with an emphasis on key regulatory proteins. We demonstrated that pancreatic adenocarcinoma (PAAD) tissues significantly enhanced ketogenic and ferroptosis phenotypes compared to normal tissues, both correlating with poorer patient prognosis. These phenotypes showed strong interdependence mediated by CAV1. In the pancreatic tumor microenvironment, CAV1 was predominantly expressed in tumor cells. Through in vitro cell experiments, we clarified that Na-OHB downregulated CAV1 expression in pancreatic cancer cells, inhibiting the transcription of the CAV1/AMPK/NRF2 downstream ferroptosis-protective genes SLC7A11 and SLC40A1. Additionally, we demonstrated the interaction between CAV1 and SLC7A11 molecules; when CAV1 was downregulated, it affected the stability of SLC7A11, leading to the ubiquitination and degradation of the translated SLC7A11 protein. Through these dual mechanisms, Na-OHB caused Fe 2+ overload, lipid peroxidation accumulation, and oxidative stress in pancreatic cancer cells, ultimately triggering ferroptosis. In ketogenic diet-fed tumor-bearing mouse models, we also observed a significant increase in lipid peroxidation and other related biomarkers, while CAV1 and SLC7A11 levels were markedly decreased compared to the normal diet group. Our findings identify CAV1 as a pivotal molecular link between ketogenic metabolism and ferroptosis in pancreatic cancer. The multi-level regulatory axis involving CAV1-mediated transcriptional regulation and post-translational modifications provides mechanistic insights into ketogenic diet-induced ferroptosis, suggesting potential therapeutic targets for pancreatic cancer adjuvant treatment.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-12
·
IF 3.9
Ma Hui,Qin Ruixiu,Yao Qiufeng,Li Yier,Cong Xingshun,Wu Wenhui,Zhao Qi,Ye Hua,Wu Kefeng
Optical transparency methods can facilitate biological tissue optical imaging, which enabled accurate three-dimensional (3D) signal visualization and quantification of complex biological structures. Unfortunately, existing optical clearing approaches present a compromise between maximizing clearing capability, the preservation of fluorescent protein emission and the speed of sample processing. To address this challenge, we synthesis of a 2-hydroxyethyl methacrylate (HEMA)-acrylamide (AAm) copolymer using antipyrine (ATP) and 2,2′-thiodiethanol (TDE) as solvent, which could embed tissue samples rapidly and highly transparent, and compatible with multiple fluorescence labeling. It can enable volumetric imaging of tissue up to the scale of mice organs, shrinkage duration of the clearing and preserve emission from fluorescent proteins and dyes. This copolymer with suitable toughness and plasticity allows the tissue of interest to be sectioned into thin slices, and histological techniques provide high-resolution two-dimensional (2D) images of cells and subcellular structures. Furthermore, HEMA-AAm copolymer -tissue transparent could distinguish cell structures between healthy and diabetic disease in dye-labeled liver tissues, which provides new insights into pathological diagnosis and analysis. Copolymer provides an environment to facilitate high-resolution 3D/2D fluorescence imaging, which enables the study of cellular and tissue morphology in experimental and clinical conditions of interest.
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RARE METALS
·
2025
·
IF 11
Ren Xiao-Qian,Chen Meng-Xin,Cao Xun,Dai Yu-Long,Yu Wen-Xiao,Liu Zhe,Wen Xin-Xin,Ma Guo-Xin,Guo Bing-Rong,Tan Sze-Xing,Liu Jia,Xu Ping,Gao Jia-Jian,Li Si-Wei
Metal oxohydroxides (MOOH) are widely accepted as the true active species for oxygen evolution reaction (OER). However, the MOOH converted from pre-catalysts usually exhibits better catalytic performance than those directly synthesized. The underlying structural reason for this phenomenon remains controversial. In this work, CoOOH and Co(OH) 2 with similar morphology are employed as model catalysts to investigate the origin of in-situ converted catalyst’s high activity, as Co(OH) 2 can be fully converted to CoOOH during OER. In-situ Raman, electron paramagnetic resonance, HR-TEM, and X-ray spectroscopic studies reveal that O vacancies in the CoOOH converted from Co(OH) 2 play a key role in its higher intrinsic activity towards OER than directly synthesized CoOOH. Furthermore, theoretical calculations and electrochemical methods indicate that O vacancies in CoOOH affect the interaction between Co–O bond, downshift the d-band center of Co, further weaken the adsorption of OH*, and finally facilitate the OER process over CoOOH. This work not only provides a deep understanding of pre-catalyst’s high OER activity by taking Co(OH) 2 as an example but also deliver insights into the activation process of other electrochemical oxidation reactions. Graphical
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-13
·
IF 3.9
Mobarak Syed Husne,Basit Abdul,Wu Lei,Khurshid Aroosa,Gui Shun-Hua,Hu Chao-Xing,Liu Tong-Xian
Bactrocera dorsalis is a major pest causing economic losses in fruit and vegetable production. Current control methods, such as synthetic pesticides and male annihilation techniques, have drawbacks, including health risks, resistance development, and ineffectiveness against females. This study investigates the attractions and oviposition stimulant activities of five common volatiles—[isobutyl acetate (A), isoamyl acetate (B), isobutyl butyrate (C), isoamyl isovalerate (D), and isoamyl butyrate (E)]—from the most preferred hosts: mango, guava, orange, and banana. These volatiles were tested at three different concentrations, both individually and in complex blends. Choice tests were performed using a Y-tube olfactometer and Petri plate with agarose assays, and antennal responses were determined using electroantennogram (EAG) recordings. Among them, a blend comprising 1.25% isoamyl acerate (10.95 µg/µl), 1.25% isoamyl isovalerate (10.75 µg/µl), and 20% isoamyl butyrate (172.40 µg/µl) (B1D1E3) emerged as the most potent attractant for both sexes and oviposition stimulant for females. Moreover, combining B1D1E3 with mango, banana, or orange juice significantly enhanced egg-laying behavior. Electrophysiological recordings supported these findings, with B1D1E3 eliciting the strongest antennal responses. Results suggest B1D1E3 could be used to elicit strong oviposition responses in mass rearing of B. dorsalis for the Sterile Insect Technique.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 5
·1-13
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IF 5.3
Zhang Jing,Yin Zhaojiang,Xiong Wei,Fan QiQi,Yu Fengyao,Liao Fusheng,Fan Hao,Qu Fei,Yu Qiangqiang
A novel, low-toxicity Ru-Ni-BPY metal–organic framework (MOF) nanomaterial was developed and a simple and sensitive dual-mode "on–off" ferric ions (Fe 3+ ) sensing platform based on this material constructed. The dual-mode detection method enabled cross-validation of the electrochemiluminescence (ECL) and fluorescent (FL) signals, enhancing accuracy and effectively minimizing errors. In linear ranges of 0.005–10 μM and 0.078–10 μM, Fe 3+ can dramatically quench the ECL and FL signals of the Ru-Ni-BPY MOF, with quenching rates of 91.42% and 91.31%, respectively, and detection limits of 0.1 nM and 9.3 nM, respectively. This ECL method not only achieved efficient detection of Fe 3+ in rat brain microdialysis fluid but also successfully enabled real-time FL detection of Fe 3+ in nude mice. Therefore, this platform has great potential for applications in environmental protection, life sciences, and early disease diagnosis. Graphical abstract
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危险品化学品经营许可证（带存储）