Food Biophysics
·
2024
·Vol. 20
·期 1
·1-13
·
IF 2.8
Chen Xing-Shuo,Yang Jie,Mao Yi-Nuo,Sun Gui-Jin,Li Ying-Qiu,Wang Chen-Ying,Liang Yan,Zhao Xiang-Zhong,Mo Hai-Zhen
This study investigated the bitterness, structure, physicochemical properties and functional properties of peanut protein hydrolysate (PPH) debittered using β-CD and 2-butanol. The sensory properties of debittered PPH were characterized using sensory evaluation combined with electronic tongue and colorimetric analysis. High-performance size exclusion chromatography (HPSEC) and Fourier transform infrared (FTIR) spectroscopy characterized the structures of debittered PPH. Surface hydrophobicity, components of amino acids, and DPPH and ABTS radical scavenging capacities characterized the functional and physicochemical properties of debittered PPH. Bitterness of PPHF-4 with combined treatment of β-CD and 2-butanol was negligible with bitterness score (0.24) and almost unchanged color. Moreover, PPHF-4 retained a high level of umami with the electronic tongue umami response value (12.95). Surface hydrophobicity and amino acid analyses showed that PPHF-4 had the lowest surface hydrophobicity (8.36) and hydrophobic amino acids (202.62 mg/g) content among PPHs debittered. HPSEC analysis showed that compared to untreated PPH, protein hydrolysates less than 5.57 kDa in PPHF-4 were obviously reduced and less than 1.68 kDa were completely removed. The DPPH and ABTS radical scavenging IC50 of PPHF-4 was 0.67 and 0.40 mg/mL, which was decreased but still had a high level of antioxidant properties compared to PPH (0.44 and 0.34 mg/mL).
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Minghui Cai,Haixu Yao,Mengnan Li,Yan Yan,Yang Cao,Jiaxin Li,Huiyu Su,Wenwen Zhang,Hui Zhu,Feiyun Cui
β-estradiol is one of the most active hormones in steroids, and it plays a vital role in the human reproductive and non-reproductive systems. Compared with other methods for detecting β-estradiol, electrochemical biosensors are economical, simple, sensitive, and rapid. Herein, spinel copper ferrite nanoparticles and laser-induced graphene (LIG) were employed to functionalize screen-printed carbon electrodes (SPCE) for the first time, and it demonstrated that they have a synergistic effect on the catalysis of β-estradiol. Differential pulse voltammetry (DPV) was used to detect the changes in the peak current generated by the oxidation of β-estradiol. The nanoparticles were characterized by Fourier infrared spectroscopy, transmission electron microscope, scanning electron microscopy, X-ray photoelectron spectroscopy (XPS), and X-ray Diffraction (XRD), and the composition elements and morphology of the nanoparticles were explained. The linear range of β-estradiol detected by this method is 0.1 ∼ 100 μM, R 2 = 0.9992, and the detection limit is 3 nM. The sensor has good selectivity, reproducibility, repeatability, stability, and anti-interference performance. In rat serum, the recovery of the sensor at 100, 10, and 1 μM β-estradiol was 105 % ∼ 110 %, with relative standard deviations of 2.3 % ∼ 4.0 % (N = 3). This study shows that the sensor can be applied to detect actual samples and has potential application value for female reproductive-related diseases.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Linlin Bian,Xue Ge,Senwei Feng,Guangxuan Chen,Kefeng Li,Xu Wang
We developed a method for the determination of chloropropanol esters and glycidyl esters (GE) in instant noodles using solid-phase microextraction with chitosan-β-cyclodextrin (CS-β-CD) coated fiber coupled with gas chromatography-tandem mass spectrometry. The developed low-cost fiber coating can improve the sensitivity of the method. Immobilized enzymes can improve operational stability and reusability compared to free enzymes, thereby reducing costs. The adsorption isotherm was modeled using the Langmuir model, while the adsorption kinetics followed the pseudo second-order model. The limit of detection was 0.3 ng/L. The method exhibited satisfactory recoveries for the analytes, ranging from 80.2 % to 105.3 %, with relative standard deviations < 9.9 %. Furthermore, the results of the exposure assessment showed that chloropropanol esters do not pose unacceptable risks to different age groups. However, the margin of exposure for GE suggested a potential health risk for populations between the ages of 3 and 12 years old.
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Journal of Functional Foods
·
2024
·
IF 3.8
Xilin Jiang,Jian Tang,Zhiqiang Gao,Caixia Chen,Wenjun Li,Lu Peng,Hongwei Li,Hongying Zhao,Yun Zhao
Moringa oleifera Lam. ( M. oleifera ) has been used as a remedy for diabetes clinically. To discover the underlying mechanism, we investigated its hypoglycemic compounds and shed light on its regulatory targets and pathways using network pharmacology. A total of 13 active compounds of stem were shot, and they may relieve diabetes through 122 core intersecting targets, mainly regulating the biological processes of inflammatory response and apoptosis. Molecular docking showed binding advantages of the active compounds with proteins in apoptosis process. In experiments conducted on MIN6 cells, an islet β-cell line, the extract of M. oleifera stem was validated to protect cells from apoptosis through reducing cellular ROS and endoplasmic reticulum stress. And consistently with its protective effect on islet β-cells, M. oleifera stem alleviated hyperglycemia in Type 2 diabetes mellitus (T2DM) mice. These results offer novel evidence for the development of functional foods to treat T2DM with M. oleifera stem.
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DIAMOND AND RELATED MATERIALS
·
2024
·
IF 4.3
YuXuan Xiong,Dandan Luo,Guowen He,Saiwen Liu,Jin Zhang,Chao Chen
Baicalin is a principal component of traditional Chinese medicine and plays a crucial role in treating conditions such as arthritis, respiratory tract infections, chronic and acute hepatitis, and tumors. Graphene oxide is bonded to silicon dioxide (SiO 2 ) through covalent bonds or hydrogen bonds and then reduced to SiO 2 @rGO through hydrazine hydrate to solve the problem of easy accumulation of SiO 2 and improve the conductivity of the material. Next, β-cyclodextrin (β-CD) self-assembles on SiO 2 @rGO to form a stable inclusion complex. The hydrophobic inner cavity of β-CD can effectively accommodate various guest molecules to form inclusion complexes, enhancing molecular recognition and detection capabilities. Finally, β-CD/SiO 2 @rGO is bridged and surface-loaded between clay-like Ti 3 C 2 T x (MXenes) nanosheets through electrostatic interactions to form a stable three-dimensional composite material. It is precisely because of MXenes, due to their unique layered microstructure, excellent conductivity, and extensive hydrophilic surface area, that they are conducive to self-assembly or embedding nanoparticles in aqueous solution. The composite was characterized via X-ray diffraction, X-ray photoelectron spectroscopy, and scanning electron microscopy. Under optimized conditions, the electrochemical sensor constructed from this composite exhibited a broad detection range (0.008–10 μM) and a low detection limit (0.191 nM) for baicalin. In comparison to existing methods for detecting baicalin, this approach demonstrates strong anti-interference capabilities and stability. It can efficiently, sensitively, and accurately quantify baicalin in Chinese patent medicine samples, including Scutellaria baicalensis root, Shuanghuanglian oral liquid, and Bear Bile Scutellaria baicalensis eye drops, achieving a satisfactory recovery rate. This study introduces a novel approach for the electrochemical detection of baicalin.
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Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Changzhe Zhou,Haojie Li,Longlong Wang,Tao Fu,Guangli Liu,Xiaoran Shen,Huazhang Zhao
Polyester membranes are increasingly utilized in wastewater treatment due to their chlorine resistance. Nonetheless, they face challenges such as limited permeability. The integration of covalent organic frameworks (COFs) can significantly enhance the performance of nanofiltration membranes. However, the heterogeneous dispersion of COFs in solution results in poor compatibility with polyester membranes, leading to increased membrane defects and compromising nanofiltration performance. This study presents the development of COFs-polyester hybrid membranes via a simple one-step in-situ co-polymerization method. During the interfacial polymerization (IP), p-phenylenediamine (Pa) and 1,3,5-triformylphloroglucinol (Tp) were integrated into a β-cyclodextrin (β-CD) aqueous solution and a trimesoyl chloride (TMC) organic solution, respectively. The uniform dispersion of COFs monomers within the IP monomer solution addressed heterogeneity issues, improving compatibility with polyester membranes. The in-situ co-polymerized COFs not only enhanced the sieving capabilities but also improved the hydrophilicity, and free volume of the membranes. The optimized membranes demonstrated a permeance of 105 L/m 2 ·h·bar, with rejection rates of 98.5 % for Congo red and 6 % for Na 2 SO 4 . Moreover, these membranes exhibited remarkable resistance to chlorine, organic solvents, and antifouling capabilities. This study offers novel insights into the application of COFs in polyester membranes, providing a straightforward, cost-effective, and scalable integration strategy.
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GREEN CHEMISTRY
·
2024
·
IF 9.3
Jie Li,Pengpeng Shao,Weijie Geng,Peng Lei,Jing Dong,Yingnan Chi,Changwen Hu
The oxidative depolymerization of β-O-4 linkages into value-added aromatic chemicals is of great importance for lignin valorization. However, achieving highly selective and rapid cleavage of β-O-4 linkages under mild conditions remains a challenge. Herein, cobalt–cobalt oxides (Co-CoOx) supported on N-doped carbon (CoMA/C900) combined with a green and recyclable base K7HNb6O19 (KNb6) have shown remarkable activity for the one-step oxidative cleavage of lignin β-O-4 linkages. Under relatively mild conditions (100 °C, 0.2 MPa O2), a lignin β-O-4 alcohol model compound was almost completely converted within 3 h, affording a narrow product distribution of phenol (yield: 99%) and methyl benzoate (yield: 98%). Based on the control experiments, kinetic study, and spectroscopic analysis, a synergistic oxidative cleavage mechanism was proposed: CoMA/C900 activates molecular oxygen to form a superoxide radical, while basic KNb6 promotes deprotonation of secondary alcohol, and they synergistically catalyze the oxidation of β-O-4 alcohol and the rapid cleavage of Cβ–O and Cα–Cβ bonds. During the reaction, the oxidation of β-O-4 alcohol to β-O-4 ketone is the rate-determining step, while the cleavage of PP-one to phenol and methyl benzoate can be completed within 15 min. Moreover, the CoMA/C900–KNb6 catalyst is recyclable at least three times and highly active for the oxidative cleavages of other lignin models and organosolv birch lignin.
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Analytical Methods
·
2024
·Vol. 16
·期 25
·4104-4115
·
IF 2.7
Hong Cui,Qiuju Tian,Yunfeng Liu,Liangpo Liu,Xiangjun Li,Jisheng Nie,Ruiping Zhang
Fluoroquinolone (FQ) antibiotics, one of the leading environmental pollutants, have ecotoxic effects that can accumulate through ecosystems and harm human health. The determination of FQs is still difficult due to the complex matrix, many interfering factors, and low concentration. Hence, a magnetic microporous organic network (MON) composite denoted as Fe3O4@MON-NH2@CM-β-CD with excellent FQ adsorption performance was prepared by β-CD covalent modification of a MON. Based on the existence of π–π packing, hydrophobic interaction, and hydrogen bonding between Fe3O4@MON-NH2@CM-β-CD and FQs, a new magnetic solid phase extraction (MSPE) method for the enrichment of FQs was developed. Under optimized MSPE conditions, five FQs were detected by HPLC-UV with good linearity (R2 ≥ 0.9989) in the range of 0.02–1 μg mL−1, and detection limits (S/N = 3) in the range of 0.0014–0.0023 μg mL−1. The satisfactory recoveries ranged from 93.1 to 116.2% with RSDs lower than 8.39% when applied to actual environmental water samples. These results revealed that Fe3O4@MON-NH2@CM-β-CD as an adsorbent for MSPE had excellent performance for FQ extraction from real samples, and the MON material types were expanded through the functionalization of MONs, which would have great potential for further application in various analytical methods.
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JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
·
2024
·Vol. 150
·期 7
·1-19
·
IF 2.7
Chang Jing-Xia,Zhang Meng,Lou Li-Li,Chu He-Ying,Wang Hua-Qi
Purpose Kinase interacting with stathmin (KIS) is a serine/threonine kinase involved in RNA processing and protein phosphorylation. Increasing evidence has suggested its involvement in cancer progression. The aim of this study was to investigate the role of KIS in the development of lung adenocarcinoma (LUAD). Dual luciferase assay was used to explore the relationship between KIS and SOX4, and its effect on ID1/β-catenin pathway. Methods Real-time qPCR and western blot were used to assess the levels of KIS and other factors. Cell proliferation, migration, and invasion were monitored, and xenograft animal model were established to investigate the biological functions of KIS in vitro and in vivo. Results In the present study, KIS was found to be highly expressed in LUAD tissues and cell lines. KIS accelerated the proliferative, migratory and invasive abilities of LUAD cells in vitro, and promoted the growth of LUAD in a mouse tumor xenograft model in vivo. Mechanistically, KIS activated the β-catenin signaling pathway by modulating the inhibitor of DNA binding 1 (ID1) and was transcriptionally regulated by SOX4 in LUAD cells. Conclusion KIS, a target of SOX4, regulates the ID1-mediated enhancement of β-catenin to facilitate LUAD cell invasion and metastasis.
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PHYTOMEDICINE
·
2024
·
IF 6.7
Huizhen Tian,Qigang Pan,Jianfeng Wu,Juanjuan Liao,Yuwei Wan,Ke Pei,Qiong Liu,Lingbing Zeng,Yanli Cao,Qiaofa shi,Nanzhen Kuang,LiJuan Sun,Xiaomin Yu,Xiaotian Huang
Background Coxsackievirus B3 (CVB3) is a leading cause of viral myocarditis and is currently lacking specific pharmacological treatments, highlighting the critical need for therapeutic development. Icariin (ICA), a prenylated flavonol glycoside, was previously found to exhibit several pharmacological effects, but its potential to combat CVB3 remains uninvestigated. Purpose This study aimed to elucidate the anti-CVB3 efficacy of ICA and elucidate its molecular mechanisms. Methods CVB3-infected HeLa cells, H9C2 cells and neonate rat ventricular cardiomyocytes (NRVCs) were selected as in vitro models, and were treated with ICA at 1 and 10 μM. Additionally, BALB/c mice that were infected with CVB3 via intraperitoneal injection were chosen as in vivo model and were treated with ICA or ribavirin over 3 days. The effect of ICA against CVB3 was determined by Cell Counting Kit-8 (CCK-8) assay, western blot, real-time fluorescence quantitative PCR (RT–qPCR), terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, hematoxylin and eosin (H&E) staining, immunohistochemistry (IHC) and flow cytometry. Results In this study, it was found that ICA is capable of reducing CVB3 viral load both in vitro and in vivo . Mechanistic studies suggested that ICA prevents cardiomyocyte apoptosis by attenuating the S100 calcium binding protein A6 (S100A6)/β-catenin/c-Myc signaling pathway. Additionally, ICA inhibits the secretion of proinflammatory cytokines tumor necrosis factor alpha (TNF-α), interleukin-1beta (IL-1β) and CXC motif chemokine ligand 2 (CXCL2) in heart tissue, thereby mitigating CVB3-induced myocarditis. Moreover, ICA also regulates the immune response of CD4 + T, CD8 + T and Treg cells by changing the cells numbers in spleen tissue. Lastly, ICA can reduce the load of other enteroviruses (such as CVA6, CVA16 and EV71) in rhabdomyosarcoma (RD) cells as well. Conclusion Our findings indicate that ICA provides significant protection against CVB3 infection by modulating the S100A6/β-catenin/c-Myc signaling pathway, suggesting its potential use as a novel drug against CVB3 infection in clinical application.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Jinpeng Li,Liang Li
Nanoparticles prepared by soy protein isolate (SPI)-oat β-glucan (OG) extrudates ( E -SPI-OG) could encapsulate quercetin and improve its bioaccessibility. This study systematically investigated the binding mechanism between E -SPI-OG and quercetin in nanoparticles using multi-spectroscopic techniques. The results revealed that fluorescence quenching via static type occurred during the interaction between E -SPI-OG and quercetin, accompanied by the occurrence of non-radiative energy transfer (binding distance was 2.99 nm and less than 7 nm). The interaction between E -SPI-OG and quercetin was an endothermic and spontaneous binding process (ΔH > 0 and ΔG < 0) and mainly driven by hydrophobic interactions (ΔH of 4.92 kJ·mol −1 and ΔS of 121.39 J·mol −1 ·K −1 ). Tryptophan and tyrosine residues of E -SPI-OG were involved in binding to quercetin, resulting in a larger binding constant (2.07–5.48 × 10 5 L·mol −1 ) and more binding sites (1.15–1.25). Quercetin altered the secondary structure of E -SPI-OG (α-helix content was reduced from 8.9 % to 6.75 %), causing its structure to become loose.
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Journal of Energy Storage
·
2024
·
IF 8.9
Baojin Jia,Qianyun Zhou,Leiying Gao,Lulu Wang,Mingyang Liu,Shiwu Xu,Genlin Zhang
The insufficient energy density of electrode materials has hindered the popularity and application of supercapacitors. The construction of hybrid supercapacitors offers an ideal solution by combining transition-metal hydroxides with high specific capacity and biochar with excellent conductivity. In this study, an electrode material (β-Ni(OH) 2 @AC 22 composite) was created by in situ growth flower-like β-Ni(OH) 2 nanosheets on biochar (AC) that extracted from the seed coat of Xanthoceras sorbifolium Bunge . This β-Ni(OH) 2 @AC 22 composite exhibited a remarkable specific capacity of 617.5C g −1 (1 A g −1 ), nearly four times that of AC, and remarkable cycling stability (85.6 % retention after 5000 cycles). Using this composite as the positive electrode and biochar as the negative electrode, the assembled battery-type hybrid supercapacitors (HSCs) achieved a specific capacity of 92.75C g −1 (1 A g −1 ). The HSCs demonstrated a specific energy of 57.9 Wh kg −1 , a specific power of 750.9 W kg −1 , and even maintained 42.2 Wh kg −1 at a maximum specific power of 6049.4 W kg −1 . The device maintained a specific capacity of about 78.7 % after 7000 charging/discharging cycles, demonstrating good practical performance. These results highlight the practical application potential of the prepared composites in supercapacitors, with biochar serving as a basis for enhancing the electrochemical performance of supercapacitors.
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危险品化学品经营许可证（带存储）