ACS Applied Materials & Interfaces
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2022
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IF 10.383
Liang Xu,Da-Wen Sun,You Tian,Libin Sun,Tianhao Fan,Zhiwei Zhu
Excessive solar radiation and high temperature often cause considerable loss and waste of fruits during transportation, retail, and storage. In the current study, a natural deep eutectic solvent-based polyacrylamide/poly(vinyl alcohol) hydrogel with nanoparticles (NPs/NADES@PAAm/PVA) is developed for fruit quality protection from solar radiation and high-temperature stress by achieving the combined effect of radiative and evaporative cooling. NPs/NADES@PAAm/PVA presents an average solar reflectance of ∼0.89 and an average emittance at the atmospheric window of ∼0.90. Besides, NPs/NADES@PAAm/PVA possesses excellent flexibility, robust mechanical strength, and good swelling behavior. The fruit preservation experiments under sunlight demonstrate that the pear (Pyrus sinkiangensis) treated with NPs/NADES@PAAm/PVA can achieve an average temperature decrease of ∼15.3 °C after sun exposure compared with the blank, and its quality-related attributes, including color, total soluble solid, relative conductivity, and respiration rate, are similar to the fresh one. Multivariate data analyses, including principal component analysis and cluster analysis, further verify that the pear treated with NPs/NADES@PAAm/PVA possesses similar quality to the fresh one after sun exposure. Thus, NPs/NADES@PAAm/PVA has promising prospects for fruit transportation, retail, and storage under solar radiation in a low-operation-cost and sustainable manner.
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Drug Design Development and Therapy
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2022
·
IF 4.319
Xin-Yu Liao,Yang Lei,Song-Feng Chen,Jing Cheng,Dan Zhao,Zhi-Feng Zhang,Xin Han,Ya Zhang,Hua-Bao Liao,Yang Zhuang,Juan Chen,Hai-Bing Zhou,Qi Wan,Ying-Ying Zou
Background: The authors have recently designed a new compound bisperoxovandium (pyridin-2-squaramide) [bpV(pis)] and verified that bpV(pis) confers neuroprotection through suppressing PTEN and activating ERK1/2, respectively. Intracerebral hemorrhage (ICH) is the second most common cause of stroke and has severe clinical outcome. In this study, we investigate the effect of bpV(pis) in ICH model both in vivo and in vitro.Materials and methods: The novel drug bpV(pis) was synthesized in the Faculty of Pharmacy, Wuhan University School of Medicine. An ICH model was generated on both SD rats and cells. bpV(pis) was injected into intracerebroventricular or culture media. Western blotting was applied to test the signal pathway. To determine the effect of bpV(pis) on PTEN inhibition and ERK1/2 activation, we measured the phosphorylation level of AKT (a direct downstream target of PTEN that negatively regulates AKT) and ERK1/2. FJC, MTT, and LDH were applied to measure the cell viability. Neurobehavioral tests were performed to measure the effect of bpV(pis).Results: The in vivo results showed that intracerebroventricular administration of bpV(pis) significantly alleviates hematoma, the damage of brain–blood barrier and brain edema. The in vitro results demonstrated that bpV(pis) treatment reduces ICH-induced neuronal injury. Western blotting results identified that bpV(pis) exerts a neuroprotective effect by significantly increasing the phosphorylation level of AKT and ERK1/2 after experimental ICH. Neurobehavioral tests indicate that bpV(pis) promotes functional recovery in ICH animals.Conclusion: This study provides first and direct evidence for a potential role of bpV(pis) in ICH therapy.
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ENVIRONMENTAL POLLUTION
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2022
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IF 9.988
Lulu Wang,Laihong Shen,Yuyang Long,Dongsheng Shen,Shouxi Jiang
Uncoupling chemical looping gasification (CLG), the organic sulfur evolution was simulated and explored qualitatively and quantitatively using typical sulfur compounds on TG-MS and temperature-programmed fixed bed. The HS radical in the reductive atmosphere easier converted to H 2 S and COS. H 2 O activated the evolution of S which was stably bonded to carbon, and H 2 generated from gasification and oxidation of reductive Fe by H 2 O contributed to the release of sulfur. The proportion of H 2 S released from sulfur compounds was greater than 87% in steam gasification, and more than 60% during CLG. Oxygen carriers promoted the conversion of sulfur to SO 2 in the mid-temperature region (500 °C–700 °C), and H 2 S in the high temperature region (700 °C–900 °C). Sulfur species played a pivotal role in sulfur evolution at low temperature of CLG. The organic sulfur in mercaptan and benzyl were more easily converted and escaped than in thiophene and phenyl . The thermal stability of sulfur species, the presence of steam and OC affected the initial temperature and peak concentration of gas sulfur release as well as sulfur distribution. Consequently, CLG strengthened the sulfur evolution, and made it possible to targeted restructure the distribution of sulfur by regulating process parameters, or blending fuel with different sulfur species for emission reduction, and selective conversion of sulfur.
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JOURNAL OF ANALYTICAL CHEMISTRY
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2022
·Vol. 77
·期 9
·1141-1146
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IF 1.237
Peng Fangfang,Zhou Xin,Cheng Wanmin,Ma Jimei,Jiang Hong
Abstract Carbon dots ( CDs ) with yellow-green fluorescence were synthesized by using m -aminophenol and ethylenediamine under mild conditions and applied as a sensor for detecting cysteine ( Cys ). Cys reacted with p -nitrophenyl acrylate via Michael addition and cyclization to form p -nitrophenol ( PNP ) which had the UV absorption maximum at 405 nm. PNP quenched the fluorescence of CDs at the excitation wavelength of 420 nm based on inner filter effect. The fluorescence intensity change of CDs was used as an output signal for Cys detection. The probe detected Cys over homocysteine and glutathione in a solution with high selectivity. The system was highly sensitive to Cys with low limit of detection of 0.8 μM and showed a good linear relationship in the range of 1–50 μM. This carbon dots probe has the potential to be applied in biological analysis.
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Water
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2022
·Vol. 14
·期 18
·2811
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IF 3.53
Zhixiong Liu,Wenlei Qin,Lei Sun,Huiyu Dong,Xiangjuan Yuan,Fei Pan,Dongsheng Xia
The degradation of acyclovir (ACY) and atenolol (ATL) in the UV/peroxydisulfate (UV/PDS) process has been systematically considered, focusing on the degradation kinetics, theoretical models, and reaction pathways via applying a microfluidic UV reaction system. The removal efficiencies of ACY and ATL were >94.8%, and the apparent degradation rate constants (kobs) were 0.0931 and 0.1938 min−1at pH 6.0 in the UV/PDS system. The sulfate radical (SO4•−) and hydroxyl radical (•OH) were identified as the major reactive radicals. The pH-dependent reaction rate constants of ACY and ATL with•OH and SO4•−were measured via the competing kinetics. Meanwhile, the contributions of•OH and SO4•−for ACY and ATL degradation were calculated by the radical steady-state hypothesis, and the results revealed that SO4•−occupied a decisive position (>84.5%) for the elimination of ACY and ATL. The contribution of•OH became more significant with the increasing pH, while SO4•−was still dominant. Moreover, ACY and ATL degradation performance were systematically evaluated via the experiments and Kintecus model under different operational parameters (Cl−, Br−, HCO3−, NOM, etc.) in the UV/PDS process. Furthermore, the plausible reaction pathways of ACY and ATL were elucidated based on the Fukui function theory and ultra-performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) analysis. The UV/PDS process has been demonstrated to be an efficient and potential application for micropollutants mitigation.Keywords:UV/PDS;micropollutants;radical contribution rates;kinetic models;degradation pathways
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ACS Nano
·
2022
·
IF 18.027
Yong Yan,Min Ni,Fan Wang,Yue Yu,Xin Gong,Yue Huang,Wei Tao,Chao Li,Feng Wang
Plant biotic or abiotic stresses, such as pathogens, mechanical damage, or high temperature, can increase intracellular H2O2 concentration, damaging proteins, lipids, and DNA. Most current H2O2 detection methods require the separation or grinding of plant samples, inducing plant stresses, and the process is complicated and time-consuming. This paper constructed a metal–organic framework (MOF)-based biosensor for real-time, remote, and in situ detection of exogenous/endogenous H2O2 in plant organs through color-to-thermal signal conversion. By simply spraying horseradish peroxidase, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and the precursor of zeolite imidazolate frameworks-8 (ZIF-8), ZIF-8 biosensors were formed in situ on a plant root, petiole, or leaf. This biosensor could report sub-micromolar H2O2 in plants since the oxidation products, ABTS• +, emitted heat when they absorbed energy from near-infrared (NIR) light. Due to the plant’s low absorption in the NIR region, the ZIF-8 biosensor allowed for remote thermal sensing of H2O2 transport or biotic/abiotic stresses in plants with a high signal-to-noise ratio combining NIR laser and thermometer. Our biosensor can be used for the future development of plant sensors for monitoring plant signaling pathways and metabolism that are nondestructive, minimally invasive, and capable of real-time, in situ analysis.
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ATMOSPHERIC ENVIRONMENT
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2022
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IF 5.755
Jinwen Li,Qingcai Chen,Dongjie Guan
Atmospheric aerosols contain organic compounds that can form triplet organics ( 3 C*) excited by sunlight, which plays a significant role in the aging process of aerosols. The formation characteristics of 3 C* of aerosol components with different polarities were investigated, including highly polar water-soluble matter (HP-WSM), humic-like substances (HULIS), and methanol-soluble matter (MSM). Carbon analysis, probe method and electron paramagnetic resonance were used to investigate the photochemical properties of ambient samples. The coupling effect of generation of 3 C* and reactive oxygen species (ROS) between different aerosol components was also examined. The results showed that the 3 C* generation characteristics are strongly dependent on the polarity of these components. HULIS has the strongest generation ability of 3 C*, and the MSM contributes the most to the total generation of 3 C*. It was found that the high-energy triplet states ( E T ≥ 250 kJ mol −1 ) of HULIS and HP-WSM components account for up to 80% of the water soluble components. These 3 C* have an important contribution to the photochemically generation of ROS, and the generated ROS of different components are also different, which is determined by the chromophore composition of complex organic compounds. A tyrosine-like chromophore is the main substance leading to the formation of water-soluble 3 C*, while the highly oxidized HULIS chromophore plays a leading role in the water-insoluble component. This study also found that there is a coupling effect between HP-WSM and HULIS on 3 C* and ROS generation. The generation rate of 3 C* increases by about 40% after mixing, but the generation of 1 O 2 is severely reduced. In general, this study provided deep insights into the generation characteristics and aging impact of the triplet state on atmospheric aerosols.
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ANALYTICAL CHEMISTRY
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2022
·
IF 8.008
Chengjie Duan,Wenting Cheng,Yanheng Yao,Dayong Li,Zhongyun Wang,Yang Xiang
Aptamer-based methods have attracted increasing interest due to flexible engineering, but their generality is limited by the heterogeneity of signal transduction mechanisms. Given the fact that nonlinear and large molecules are more likely to make the nanosurface overloaded, we investigated a novel signal transduction process to extend the application of aptasensors. In this work, an aptamer complementary element (ACE) is designed with a primer region to serve as the signal probe, which can fully hybridize with an aptamer and be separated by magnetic beads (MBs). Upon target binding, the formed aptamer/target complex is much larger than the linear aptamer/ACE-primer dimer, causing overload of MBs on account of steric hindrance. An extra aptamer/ACE-primer can escape from the surface to the supernatant, which can be amplified by a catalytic hairpin assembly (CHA) circle. The size-dependent signal transduction and the modular design endow the method with high generality and flexibility for protein analysis. The proposed aptasensor was successfully applied to the detection of tau proteins ranging from 0.5 to 1000 ng mL–1 with a limit of detection (LOD) as low as 0.254 ng mL–1. The recovery tests in both human serum and cerebra spinal fluid confirmed the high accuracy and stability. Furthermore, a successful distinction was made between AD patients and healthy controls by the method, suggesting the possible applicability for practical analysis of tau proteins.
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MOLECULAR MEDICINE
·
2022
·Vol. 28
·期 1
·1-15
·
IF 6.376
Wang Bingwu,Gao Jian,Zhao Zhongjun,Zhong Xuefei,Cui Hao,Hou Hui,Zhang Yanping,Zheng Junnian,Di Jiehui,Liu Yong
Background Targeting ribosome biogenesis to activate p53 has recently emerged as a therapeutic strategy in human cancer. Among various ribosomal proteins, RPL11 centralizes the nucleolar stress-sensing pathway by binding MDM2, leading to MDM2 inactivation and p53 activation. Therefore, the identification of MDM2-binding RPL11-mimetics would be valuable for anti-cancer therapeutics.Methods Based on the crystal structure of the interface between RPL11 and MDM2, we have identified 15 potential allosteric modulators of MDM2 through the virtual screening.Results One of these compounds, named S9, directly binds MDM2 and competitively inhibits the interaction between RPL11 and MDM2, leading to p53 stabilization and activation. Moreover, S9 inhibits cancer cell proliferation in vitro and in vivo. Mechanistic study reveals that MDM2 is required for S9-induced G2 cell cycle arrest and apoptosis, whereas p53 contributes to S9-induced apoptosis.Conclusion sPutting together, S9 may serve as a lead compound for the development of an anticancer drug that specifically targets RPL11-MDM2-p53 pathway.
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International Journal of Nanomedicine
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2022
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IF 7.033
Xiao Zhao,Ying Wang,Tingting Zhu,Huili Wu,Diya Leng,Zhiguo Qin,Yan Li,Daming Wu
Background Mesoporous calcium-silicate nanoparticles (MCSNs) are advanced biomaterials that have been used to control drug delivery for many years. Ultrasmall Prussian blue nanoparticles (UPBNPs) showed high peroxidase and catalase-like activities. This study evaluated the antibacterial and antibiofilm properties, mechanism and cytotoxicity of UPBNPs-MCSNs composites synthesized by both as precursors. Methods UPBNPs-MCSNs were prepared and characterized. The antibacterial effect of UPBNPs-MCSNs was evaluated by the MTT assay and CFU counting method, and their biosafety was tested by CCK8. Then explore the antibacterial mechanism, including TEM observation of bacterial morphology, and detection of bacterial ROS, LPO and GSH levels. The antibiofilm activity of UPBNPs-MCSNs was tested by E. faecalis biofilm model in human roots. The roots were pretreated with materials and cultured with E. faecalis , and the survival of E. faecalis on the root canal wall was observed by SEM and CLSM. Results The results showed that UPBNPs-MCSNs had potent antibacterial and antibiofilm activities. They can aggregate on the dentin surface and significantly inhibit E. faecalis adhesion and colonization. Their antibacterial activity is as effective as NaClO and calcium hydroxide (CH), can significantly prolong the time of bacterial colonization than CH, but have lower cytotoxicity to normal cells. We found that UPBNPs-MCSNs trigger a like classic ferroptosis pathway in bacteria. UPBNPs-MCSNs can induce bacteria to produce ROS and LPO, and reduce GSH level. Moreover, we observed that the metal ions chelator and the antioxidant could block their antibacterial activity. Conclusion These results reveal that UPBNPS-MCSNs have high antibacterial and antibiofilm, and can mediate the bacterial redox pathway ROS/GSH like the classical pathway of ferroptosis, providing a theoretical basis for them to develop into a safe and effective novel root canal disinfectant.
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Frontiers in Sustainable Food Systems
·
2022
·
IF 5.005
Dong Yuhe,Zhong Wenting,Yang Chunmiao,Zhang Yongze,Yang Dongsheng
The objectives of this study are to optimize the ultrasonic microwave synergistic extraction (UMSE) of anthocyanins from Lycium ruthenicum Murr (ALRM) by response surface methodology and also to investigate its antioxidant activity in vitro. Based on the single-factor experiments, we optimized the ALRM extraction process by response surface methodology assuming anthocyanin extraction rate as the response point and microwave power, ultrasonic power, extraction time, and liquid-to-material ratio as the investigating parameters as well as performed correlation analysis between DPPH·, ·OH, ABTS·, O2-· radical scavenging potential, and ferric-reducing antioxidant power. The results showed that the influence on anthocyanin yield was in the order of liquid to material ratio > microwave power > ultrasonic power > extraction time; the elicited extraction conditions were: ultrasonic power 216.253 W, microwave power 89.311 W, time 26.141 min, liquid to material ratio 17.294 mL/g, the forecast content was 10.157 mg/g, while the actual value was consistent with the preview value. Meanwhile, ALRM obtainable by UMSE was identified as yielding favorable antioxidant properties firstly, when the concentration of purified ALRM (ALRM-1) was increased, the antioxidant capacity was strengthened. Theoretically, we provide a basis for the extraction procedure of ALRM and its antioxidant activity, which serves as a promising antioxidant and free radical scavenger.
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ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Danping Wang,Chaoying Du,Shuo Wang,Lingxiao Li,Tian Liu,Jiaxuan Song,Zhonggui He,Yinglei Zhai,Bingjun Sun,Jin Sun
Prodrug-based self-assembled nanoparticles combined with the merits of nanotechnology and prodrugs strategies have gradually become a research trending topic in the field of drug delivery. These prodrugs usually consist of parent drugs, connecting bonds, and modifying chains. The influences of the connecting bonds and modifying chains on the pharmaceutical characteristics, in vivo delivery fate, and antitumor activity of prodrug nanoassemblies remain elusive. Herein, three docetaxel (DTX) prodrugs were designed using sulfur bonds (thioether bond or disulfide bond) as connecting bonds and fatty alcohols (straight chain or branched chain) as modifying chains. Interestingly, the difference between connecting bonds and modifying chains deeply influenced the colloidal stability, redox responsive drug release, cytotoxicity, pharmacokinetic properties, tumor accumulation, and antitumor effect of prodrug nanoassemblies. DTX conjugated with branched chain fatty alcohols via disulfide bonds (HUA-SS-DTX) significantly improved the antitumor efficiency of DTX and reduced the systematic toxicity. Our study elaborates on the vital role of connecting bonds and modifying chains in the rational design of prodrug nanoassemblies.
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危险品化学品经营许可证（带存储）