Materials Today Communications
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2023
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IF 3.8
Shi Qiu,Fengdong Sun,Chen You,Chaokun Tang,Baoli Zhou,Shuquan Zhang,Jiangtao Feng,Aixian Tian,Minfang Chen
Through preparing the porous Zn-0.8Li scaffolds with 50–70 % porosity by using NaCl particles as pore-forming agents in the range of 150–350 µm and 350–550 µm with the infiltration casting method, the present study investigated the pore structure , mechanical properties and weight loss in simulated body fluids (SBF), and conducted the cellular activity and in vivo degradation experiments on porous Zn-0.8Li scaffolds before and after chitosan coating treatment. The results showed that the porous samples with small pore size had higher mechanical properties and lower degradation rate. However, with relatively higher average porosity and through-porosity, the porous samples with large pore size showed better pore connectivity, which provided channels for nutrient transport and promoted the lateral growth of internal tissues. In terms of cell experiments, chitosan coated Zn-0.8Li scaffolds with large pore size had better cell activity and adhesion. Since the coating of chitosan inhibited the release of Zn 2+ during the implantation of porous Zn-0.8Li scaffolds in vivo, it effectively promoted the growth of new bone tissue around the porous scaffolds, which provided an ideal microenvironment for the adhesion, proliferation and differentiation of MSCS. The results of this study demonstrated the great potential of chitosan coated porous biodegradable Zn-based scaffolds for bone tissue engineering.
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Materials Express
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2023
·Vol. 13
·期 1
·81-84
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IF 0.7
Wang Wen-Wen,Yang Li-Jie,Guo Jun-Feng
Pancreatic cancer is a common tumor of the digestive system with a high degree of malignancy and poor prognosis. Its incidence has significantly increased worldwide in recent years. Currently, approximately 90% of patients cannot be treated surgically. Thus, drug therapy plays an important
role in pancreatic cancer treatment. In this study, hydrogels loaded with mitomycin were prepared from hyaluronic acid and carboxymethyl chitosan, which have excellent biocompatibility. SEM results showed that the hydrogels had a three-dimensional interoperable porous structure, and CCK-8
results showed that hydrogels loaded with mitomycin significantly reduced cancer-cell proliferation. The hydrogels also reduced the migration of cancer cells in a dose-dependent manner, indicating their potential use as a drug-carrier material for pancreatic cancer treatment.
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SCIENCE OF THE TOTAL ENVIRONMENT
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2022
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IF 10.753
Xianliang Wu,Huijuan Liu,Xuetao Guo,Zhenming Zhang,Jiachun Zhang,Xianfei Huang
An increasing number of researchers have focused on microplastics (MPs) pollution in inland freshwater lakes due to its ecotoxicity, while little is known about the effects of hydrological periods on MPs distribution. Therefore, this study aims to investigate MPs distribution, morphological characteristics and physicochemical indices in various environments in dry and wet periods in Caohai Lake. The results exhibited that cultivated soil, water, and sediment in Caohai Lake have been polluted by MPs in dry and wet periods. There were pellets, fragments, film, and fibers of MPs in both dry and wet periods, and MP foam was additionally found in the wet period. MPs with 0 to 0.5 mm possessed the largest proportion in the five environments in dry and wet periods, followed by MPs with 0.5 to 1 mm and 1 to 5 mm. In Caohai Lake, the black, white, green, red, and transparent MPs in dry period, and black, colourful, grey, red and transparent MPs in wet period were found. The developed structural equation model confirmed that MPs in sediment were probably mainly from soil. There are negative effects of the relative abundance of MPs from cultivated soil to lake water in the dry period, whereas the opposite is true in the wet period. Interestingly, the complex and fast water velocity in the estuary in the wet period led to a lower relative abundance of MPs in its sediment in comparison with the dry period. The distribution model of MPs in estuary and lake water in dry and wet periods is not inconsistent. Our results suggest that the related government department should take measures to reduce the MPs pollution in Caohai Lake, especially from the source.
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International Journal of Nanomedicine
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2023
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IF 8
Yanan Dong,Rongzhan Fu,Jing Yang,Pei Ma,Lihua Liang,Yu Mi,Daidi Fan
Background and purpose Ginsenoside Rg5 (Rg5), a triterpene saponin, extracted from the natural herbal plant ginseng, is one of the most potent anticancer drugs against various carcinoma cells. However, the therapeutic potential of Rg5 is limited by its low solubility in water, poor bioavailability, and nontargeted delivery. Therefore, we prepared folic acid (FA)-modified bovine serum albumin (BSA) nanoparticles (FA-Rg5-BSA NPs) to improve the therapeutic efficacy and tumor targetability of Rg5.Methods Various aspects of the FA-Rg5-BSA NPs were characterized, including size, polydispersity, zeta potential, morphology, entrapment efficiency (EE), drug loading (DL), in vitro drug release, thermal stability, in vitro cytotoxicity, cell apoptosis, cellular uptake, in vivo antitumor effects and in vivo biodistribution imaging.Results The FA-Rg5-BSA NPs showed a particle size of 201.4 nm with a polydispersity index of 0.081, uniform spherical shape, and drug loading of 12.64±4.02%. The aqueous solution of FA-Rg5-BSA NPs had favorable stability for 8 weeks at 4°C. The FA-Rg5-BSA NPs dissolved under acidic conditions. Moreover, the Rg5-BSA NPs and FA-Rg5-BSA NPs had advanced anticancer activity compared with Rg5 in MCF-7 cells, while poor cytotoxicity was observed in L929 cells. The FA-Rg5-BSA NPs facilitated cellular uptake and induced apoptosis in MCF-7 cells. In addition, in an MCF-7 xenograft mouse model, the in vivo antitumor evaluation revealed that FA-Rg5-BSA NPs were more effective in inhibiting tumor growth than Rg5 and Rg5-BSA NPs. The in vivo real-time bioimaging study showed that the FA-Rg5-BSA NPs exhibited superior tumor accumulation ability.Conclusion The results suggested that FA-Rg5-BSA NPs could serve as a promising system to improve the antitumor effect of Rg5.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2022
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IF 9.136
Xingzhen Zhang,Ying Liu,Feng Zhang,Wangxi Fang,Jian Jin,Yuzhang Zhu
Developing a membrane integrating high separation efficiency and good anti-fouling ability for both oil-in-water and water-in-oil emulsions is meaningful due to the complexity and diversity of actual oily wastewater. Herein, a facile strategy was designed to generate a nanofibrous Janus membrane to achieve multifunctional and self-cleaning properties. Hydrophilic β-FeOOH was anchored on poly(vinylidene fluoride-co- hexa -fluoropropylene) (PVDF-HEP) fibers to achieve hydrophilicity and self-cleaning performance of PVDF-HEP/β-FeOOH layer by in situ mineralization technology. The Janus membrane was then obtained by incorporating PVDF-HEP fibers layer onto the hydrophilic layer through electrospinning technology. PVDF-HEP/β-FeOOH layer owns superhydrophilicity (WCA = 0°) and underwater superoleophobicity (OCA = 159°), which can separate different surfactant-stabilized oil-in-water emulsions with separation efficiency of above 99.8 % when this layer is facing up. Conversely, the PVDF-HEP layer exhibits superhydrophobicity (WCA = 149°), which can separate different surfactant-stabilized water-in-oil emulsions with separation efficiency of above 99 % when this layer is facing up. More importantly, a satisfying water flux recovery rate of 98.2 % is acquired due to the synergistic effect between β-FeOOH photocatalysis and asymmetric wettability of the membrane, endowing the membrane with effective self-cleaning capability and satisfactory stability. This study provides a new strategy for designing multifunctional membrane toward actual complex oily wastewater treatment .
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Hong Lin,Jianbin Tan,Jing Wang,Chengliang Xie,Bifeng Chen,Mansi Luo,Yun Liu,Wenzhen Liao,Weiling Huang,Hongxia Wang,Ying Jiang,Kexin Wang,Ciyong Lu,Min Zhao
The widespread use of titanium dioxide nanoparticles (TiO2 NPs) in the food industry has brought about human safety risks related to nanotoxicity. In this study, food-related TiO2 NPs (anatase, 40 nm) were given to rats by oral gavage for 90 days at doses of 10, 100, and 1000 mg/kg bw. An additional two satellite groups underwent the same protocol for 45 days and for 90 days followed by a 28 day recovery. TiO2 NPs tended to agglomerate together in H2O, AGJ, and AIJ. No systemic toxicity was observed after 90 day agglomerated TiO2 NP exposure with no Ti distribution in major tissues/organs. Furthermore, TiO2 NP consumption for 90 days had no impact on microbiota diversity; the community structure of the gut microbiota is shifted to some extent at the genus level. Collectively, the NOAEL of agglomerated TiO2 NPs for 90 days of oral administration was 1000 mg/kg bw, the highest dose tested in male and female rats.
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CERAMICS INTERNATIONAL
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2023
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IF 5.2
Jing Duan,Tao Lin,Hongyuan Liu,Jing Xu,Huiping Shao
Polyvinylalcohol/chitosan (PVA/CS) is an excellent dual-network hydrogel material, but some significant challenges remain in fabricating composites with specific structures. In this study, 3D gel printing (3DGP) combined with a water-level controlled crosslinker bath was proposed for the rapid in-situ prototyping of PVA/CS/Fe 3 O 4 magnetic hydrogel scaffolds. Specifically, the PVA/CS/Fe 3 O 4 hydrogels were extruded into the crosslinker water to achieve rapid in-situ gelation , improving the printability of hydrogel scaffolds. The effect of the PVA/CS ratio on the rheological and mechanical properties of dual-network magnetic hydrogels was evaluated. The printing parameters were systematically optimized to facilitate the coordination between the crosslinking water bath and printer. The different crosslinking water baths were investigated to improve the printability of PVA/CS/Fe 3 O 4 hydrogels. The results showed that the printability of the sodium hydroxide (NaOH) crosslinker was significantly better than that of sodium tripolyphosphate (TPP). The magnetic hydrogels (PVA: CS= 1: 1) crosslinked by NaOH had better compressive strength , swelling rate, and saturation magnetization of 1.17 MPa, 92.43%, and 22.19 emu/g, respectively. The MC3T3-E1 cell culture results showed that the PVA/CS/Fe 3 O 4 scaffolds promoted cell adhesion and proliferation, and the scaffolds crosslinked by NaOH had superior cytocompatibility. 3DGP combined with a water-level controlled crosslinker bath offers a promising approach to preparing magnetic hydrogel materials.
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Pharmaceutics
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2023
·Vol. 15
·期 2
·388
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IF 5.4
Kunfeng Zhang,Jingxin Fu,Xiaorui Liu,Yifei Guo,Meihua Han,Meifeng Liu,Xiangtao Wang
Mitochondria are involved in various stages of cancer cell diffusion and metastasis. Therefore, targeting tumor mitochondria with antineoplastic medicines to cause mitochondria to initiate apoptosis may be an effective strategy for cancer therapy. Here, in order to enhance the anti-tumor efficacy of the antineoplastic agent hydroxycamptothecin (HCPT), the mitochondrial targeting ligand 4-(carboxybutyl) triphenylphosphine bromide (TPP) was attached to HCPT by an ester linkage. The resultant TPP-HCPT (TH) conjugate could self-assemble into nano-aggregates, with a mean particle size of 203.2 nm and a polydispersity index (PDI) value of 0.312. The TH conjugate could also co-assembly with mPEG3000-PLGA5000into nanoparticles (TH-NPs), with a mean diameter of 86.41 nm, a PDI value of 0.256 and a zeta potential of −0.125 mV. In contrast to HCPT injections, TH aggregates displayed enhanced cellular uptake, mitochondria-targetability and cytotoxicity against 4T1 cells, while TH-NPs showed even better improvement than TH aggregates. In the in vivo study, TH aggregates displayed higher anti-tumor efficacy in 4T1 tumor-bearing mice than HCPT injections (tumor inhibition rate, 55.71% vs. 69.17%), and TH-NPs displayed more superior anti-tumor effects (tumor inhibition rate, 80.02%). In conclusion, our research demonstrated that the TPP-HCPT conjugate and its nano-formulations, including TH aggregates and TH-NPs, may be a promising mitochondria-targeting anticancer medicine for cancer therapy. As far as we know, this is the first report in which TPP and HCPT have been conjugated directly for this aim.Keywords:mitochondrial targeting;hydroxycamptothecin;triphenylphosphine;nanoparticles
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ENVIRONMENTAL POLLUTION
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2023
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IF 8.9
Shuxin Li,Wenjing Zhang,Dayi Zhang,Wei Xiu,Shengyu Wu,Juanfen Chai,Jincai Ma,Muhammad Yousuf Jat Baloch,Simiao Sun,Yuesuo Yang
The vadose zone is a critical zone for microbial entry into the subsurface environment, and various types of inorganic and organic colloids can affect the migration of pathogenic bacteria. In the study, we explored the migration behavior of Escherichia coli O157:H7 with humic acids (HA), iron oxides (Fe 2 O 3 ) or their mixture, uncovering their migration mechanisms in the vadose zone. The effect of complex colloids on the physiological properties of E. coli O157:H7 was analyzed based on the measured particle size, zeta potential and contact angle. HA colloids significantly promoted the migration of E. coli O157:H7, where Fe 2 O 3 was opposite. The migration mechanism of E. coli O157:H7 with HA and Fe 2 O 3 is obviously different. Multiple colloids dominated by organic colloid will further highlight its promoting effect on E. coli O157:H7 under the guidance of electrostatic repulsion due to the influence of colloidal stability. Multiple colloids dominated by metallic colloid will inhibit the migration of E. coli O157:H7 under the control of capillary force due to the restriction of contact angle. The risk of secondary release of E. coli O157:H7 can be effectively reduced when the ratio of HA/Fe 2 O 3 is ≥ 1. Combining this conclusion with the distribution characteristics of soil in China, an attempt was made to analyse the migration risk of E. coli O157:H7 on a national scale. In China, from north to south, the migration capacity of E. coli O157:H7 gradually decreased, and the risk of secondary release gradually increased. These results provide ideas for the subsequent study of the effect of other factors on the migration of pathogenic bacteria on a national scale and provide risk information about soil colloids for the construction of pathogen risk assessment model under comprehensive conditions in the future.
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BRAIN RESEARCH BULLETIN
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2023
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IF 3.8
Jingwei Zhao,Mingming Zhang,Huamin Zhang,Ying Wang,Bingyu Chen,Jing Shao
Diosmin is a flavone glycoside with a confirmed therapeutic effectiveness on the chronic venous disorders. In this paper, the classical mouse depression model induced by LPS was established to explore the effect of Diosmin on depression. Firstly, we found that Diosmin could inhibit the inflammation and neuronal damage in the prefrontal cortex (PFC) of mice, and thus alleviating the LPS-induced depressive-like behaviors. Specifically, Diosmin treatment significantly suppressed the secretion of pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β), reduced the activation of microglia, and inhibited the expression of NLRP3 inflammasome and its downstream effector caspase-1 in both PFC of mice and BV2 microglial cells exposed to LPS. Then, we demonstrated that pretreatment with Diosmin dramatically suppressed the LPS-induced oxidative stress in the PFC of mice, manifested in the decrease of reactive oxygen species and malondialdehyde while increase of catalase activity. Consistently, Diosmin also alleviated the oxidative stress in BV2 cells exposed to LPS. Finally, we confirmed that Diosmin effectively suppressed the activation of NF-κB signaling pathway in the PFC of LPS-treated mice. Further in vitro experiments also verified that Diosmin could prevent the p65 transposition to nucleus in LPS-treated BV2 cells, suggesting that the antidepressant effects of Diosmin are partially mediated by blocking of NF-κB signaling. Taken together, this study proposes the potential antidepressant effect of Diosmin, which provides useful support to the development of new therapies for depression.
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JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
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2023
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IF 6.1
Yiqian Zhu,Hui-Fang Xiao,Meisheng Li,Shouyong Zhou,Haq Navaz,Ailian Xue,Chao-Nan Wang,Rong Xu,Dawei Yang,Chenglung Chen,Yijiang Zhao
It has been a longstanding problem to combat the oxidation process of MXene membranes. Here, we propose a strategy to construct nano bridge defect repair loose nanofiltration membrane with the help of MXene oxidation. The oxidation kinetics of Ti 3 C 2 T x was investigated in both model and experimental aspects. The acid solution leads to aggregation and smaller d-spacing. However, the oxidation of MXene was accelerated in alkaline solution via reaction with hydroxyl anions resulting in a hydrophilic MXene membrane surface. The water permeability of the pH=12-Ti 3 C 2 T x membrane increased to a maximum value of 26.5 L·m -2 ·h -1 ·bar -1 together with a high rejection (98%) of Janus green B (JGB). Titanium dioxide, which was in situ formed by oxidation of MXene, gives the membrane self-cleaning properties under UV light. The water recovery ratio was as high as 96.6%. To further study the mechanism of in situ oxidation of MXene to form nano-titanium dioxide, the oxidation stages were analyzed which revealed that the in-situ oxidation process involved three different oxidations stages. This research offers a new perspective on MXene membrane design, paving the way for a new method of in situ nano bridge defect repair loose MXene nanofiltration membrane.
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BMC Complementary Medicine and Therapies
·
2023
·Vol. 23
·期 1
·1-14
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IF 3.9
Han Wenrun,Zhang Dandan,Zhang Peng,Tao Qianqian,Du Xiaoli,Yu Chunquan,Dong Pengzhi,Zhu Yan
IntroductionLiver X Receptor (LXR) agonists could attenuate the development of atherosclerosis but bring excess lipid accumulation in the liver. Danlou Recipe was believed to be a benefit for improving the lipid profile. Thus, it is unclear whether Danlou Recipe could attenuate hyperlipidemia without excess lipid accumulated in the liver of mice. This study aimed to clarify if Danlou Recipe could alleviate the progression of hyperlipidemia in mice without extra lipids accumulated in the liver.Methods Male murine macrophage RAW264.7 cells and murine peritoneal macrophages were used for the in vitro experiments. Cellular cholesterol efflux was determined using the fluorescent cholesterol labeling method. Those genes involved in lipid metabolism were evaluated by qRT‐PCR and western blotting respectively. In vivo, a mouse model of hyperlipidemia induced by P407 was used to figure out the effect of Danlou Recipe on reverse cholesterol transport (RCT) and hyperlipidemia. Ethanol extract of Danlou tablet (EEDL) was prepared by extracting the whole powder of Danlou Prescription from ethanol, and the chemical composition was analyzed by ultra-performance liquid chromatography (UPLC).Results EEDL inhibits the formation of RAW264.7 macrophage-derived foam cells, and promotes ABCA1/apoA1 conducted cholesterol efflux in RAW264.7 macrophages and mouse peritoneal macrophages. In the P407-induced hyperlipidemia mouse model, oral administration of EEDL can promote RCT in vivo and improve fatty liver induced by a high-fat diet. Consistent with the findings in vitro, EEDL promotes RCT by upregulating the LXR activities.Conclusion Our results demonstrate that EEDL has the potential for targeting RCT/LXR in the treatment of lipid metabolism disorders to be developed as a safe and effective therapy.
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