Acta Neuropathologica Communications
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2026
·Vol. 14
·期 1
·122
·
IF 6.5
Zhang Xiangyi,Zhang Jingjing,Zhang Yan,Wang Dan,Liu Gaixiu,Wang Mengfei,Li Chaoyang,Shi Qi,Dong Xiaoping,Yuan Chonggang,Li Wenlong,Ma Jiyan
Prion protein (PrP) is essential for the pathogenicity of prion diseases, a group of fatal neurodegenerative disorders affecting both humans and animals. The octapeptide repeats (OR) of PrP is a highly conserved structural feature; however, conflicting observations—that OR expansion causes inherited prion diseases while being dispensable for prion transmission—obscure its role in these disorders. We developed ΔOR mice by deleting the OR from the endogenous PrP-encoding gene, PRNP , which resulted in significantly prolonged survival times for mice inoculated with any of the five prion strains. Although disease characteristics were similar between terminally ill ΔOR and wild-type mice, the aggregation and conversion of PrP to the misfolded PrP Sc were substantially delayed in ΔOR mice. This delay is attributable to reduced OR-OR self-association and diminished interactions between OR and the positively charged N-terminus of PrP. Additionally, the neurotoxic phase of the disease was both delayed and significantly prolonged in ΔOR mice. Our findings demonstrate the critical roles of OR in both PrP misfolding and neurotoxicity in prion diseases, highlighting OR as a promising therapeutic target that can mitigate both essential pathogenic processes in prion diseases.
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Journal of Bionic Engineering
·
2026
·
IF 4.4
Liu Qiang
Friction electric nanogenerators based on natural biomaterials have become a research hotspot in sports tactile monitoring since their self-powering ability, biocompatibility, and flexibility. To design a green, flexible, and high-performance tactile sensor device, this study innovatively used a lithium chloride/N-methylpyrrolidone solvent system to extract β-chitin from discarded squid cartilage. A composite nanofiber membrane was constructed using electrospinning method, and a cuttlefish cartilage-based β-chitin triboelectric array was successfully prepared. The experiment demonstrated that the peak output voltage of the frictional electric array at 3 Hz frequency was 117.0 V. After 5,000 cycles, the peak voltage remained at about 116.8 V, and the waveform shape was basically the same as the initial stage, with a peak decrease of less than 1.0%. Further placement of the frictional electric array at the heel of the football insole successfully monitored changes in foot pressure under different motion states. The output currents under walking, running, and sprinting conditions were 3.2 µA, 5.4 µA, and 8.6 µA, with stable signals and high discrimination. This study demonstrates the potential application of natural β-chitin materials in flexible self-powered sensors and provides a high-sensitivity, low-cost tactile monitoring solution for intelligent training in football.
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Nature Communications
·
2026
·Vol. 17
·期 1
·4225
·
IF 18.1
Wang Yu-Meng,Jia Yanyan,Wu Yu,Zhao Qiaohang,Guo Yumeng,Zhang Li,Jin Min,Zheng Yufang
Elevated prenatal androgen levels have been associated with an increased incidence of several neurodevelopmental conditions in offspring. However, the underlying mechanisms remain poorly understood. Here we showed that elevated prenatal androgens, modeled via aromatase inhibition in mice, disrupt cortical development and behavior in male offspring by activating androgen receptors (AR). AR interacts with MeCP2 to upregulate the transcription factor MEF2C, bypassing standard androgen response elements. Knocking down Mecp2 or Mef2c , or blocking AR, reversed these effects. Mecp2 duplication alone also upregulated MEF2C, driving similar neurogenesis and behavioral changes that were reversed by AR blockade. This reveals a MeCP2-mediated, non-canonical pathway by which androgens may contribute to male-biased neurodevelopmental conditions.
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Nature Microbiology
·
2026
·
IF 18.7
Xu Yuanpeng,Kong Xiaoying,Qiao Qian,Liu Changhao,Zhu Jinyi,Wang Lei,Hu Yanhao,Sun Yujing,Qiu Min,Yan Wei,Dong Suomeng,Ma Wenbo,Wang Yan,Wang Yuanchao
Extracellular vesicles (EVs) transport biologically active molecules and are known to mediate host defence against microbial pathogens, including plant fungal pathogens. However, the mechanism by which pathogens disrupt EV-dependent defence remains unclear. Here we show that Phytophthora capsici , a global crop pathogen, counteracts EV-mediated plant defence through targeted lipase activity. We show that Arabidopsis releases EVs containing tetraspanin (TET), specifically TET8- and TET9-EVs, which damage germinated spores of Phytophthora , reducing infection. As a counter-defence, Phytophthora secretes an infection-induced apoplastic lipase, Plant Extracellular Vesicle Destroyer 1 (PED1), which targets TET8- and TET9-EVs. This occurs via interaction with the EV membrane-localized protein Defective Glycosylation 1 (DGL1), which directly interacts and co-localizes with TET8 and TET9 on the EV membrane. PED1 damages TET8- and TET9-EVs through its lipase activity towards campesteryl esters, suppressing EV-mediated plant defence. Our study reveals a mechanism used by Phytophthora to counteract EV-mediated host defence.
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PubMed
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npj Heritage Science
·
2026
·
IF 0
Li Zequn,Yuan Fang,Li Yuqi,Ma Tao,Pan Zhijun,Zhou Jianzhong,Li Weiqiang,Zhang Liangren
The production and trade of the pre-Qin prestigious goods of proto-porcelain wares has been riveting the attention of scholarly research for decades. In much of previous provenance studies, however, it has been commonly assumed that products of the major production center of Dongtiaoxi and Wuyishan are geochemically homogeneous. Our previous studies, however, have upset this assumption by identifying geochemical differences within the two centers. This study continues to explore the geochemical complexity by analyzing samples from outlying sites of the Longshan Cluster of the Dongtiaoxi Center. As a result, we find two additional complexities: a spatial dichotomy in body composition between the two banks of the Dongtiaoxi River and a diachronic evolution in glaze recipe. In the meantime, by comparing with the Wuyishan Center, we find a few fingerprint elements. Altogether, we build up a more rigorous framework for future proto-porcelain provenance studies.
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Food and Bioprocess Technology
·
2026
·Vol. 19
·期 7
·359
·
IF 5.6
Li Yanjiao,Qi Yaoyao,Deng Zeyuan,Li Guangyan,Zhang Jinwu,Li Hongyan
Ganoderma lucidum ( G. lucidum ) spore oil is a premium functional lipid, but its safety is frequently compromised by phthalate plasticizers (PAEs). Although supercritical fluid extraction (SFE) is widely used to obtain spore oil, the co-extraction of these hazardous PAEs remains a critical bottleneck. This study introduces an integrated in situ adsorption strategy within the SFE process, coupling one-step micro-interfacial purification with an AHP-CRITIC multi-criteria decision model to mathematically optimize the inherent yield-quality trade-off. The application of activated carbon (AC) and activated clay (ACLT) revealed distinct physicochemical trade-offs. For AC, its non-selective microporous network caused massive competitive physical entrapment, depleting above 70% of bioactive ergosterol despite achieving a high PAEs removal rate (77.18%). Conversely, while ACLT excellently captured PAEs via specific surface chemisorption, excessive dosages introduced abundant acidic active sites that catalyzed detrimental triglyceride hydrolysis, increasing the acid value to 22.43 mg/g. Pearson correlation analysis further corroborated these inherent conflicts, revealing a powerful co-extraction dynamic between lipophilic PAEs and ergosterol ( r = 0.879**) alongside profound negative correlations between elevated AV and bioactive retention. The AHP-CRITIC model identified 0.50 kg of ACLT as the optimal adsorbent dosage, achieving the highest comprehensive score of 80.42. Under this condition, PAEs removal reached 65.70%, while above 60% of thermosensitive ergosterol was retained and oxidative stability remained excellent. Ultimately, this integrated solvent-free method offers a practical industrial alternative for refining premium functional lipids.
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JOURNAL OF NANOPARTICLE RESEARCH
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2026
·Vol. 28
·期 6
·139
·
IF 3.5
Luo Haoyu,Liu Feng,Li Jiale,Wang Tao,Lv Lianpeng,Wang Bing
Reactive oxygen species (ROS)-mediated therapeutic modalities, including photodynamic therapy (PDT) and copper ion-mediated chemodynamic therapy (CDT), have been recognized as highly promising non-invasive strategies for cancer treatment. However, their therapeutic efficacy is frequently restricted by poor subcellular targeting capability. To address this challenge, a mitochondria-targeted nanoplatform (Cu@GO-PEG-Ce6/TPP) was developed for light-triggered ROS therapy. Driven by triphenylphosphine (TPP) moieties, selective accumulation of the nanoplatform within mitochondria was achieved. Upon single 660 nm laser irradiation, singlet oxygen ( 1 O 2 ) was generated by the photosensitizer Ce6 to execute the PDT effect, while the loaded copper ions catalyzed endogenous H 2 O 2 within tumor cells through a Fenton-like reaction, leading to in situ production of highly toxic hydroxyl radicals (•OH) to fulfill the CDT function. Additionally, mild photothermal effect (PTT) was produced by the graphene oxide scaffold, with a photothermal conversion efficiency of 22.48%, through which the Fenton-like reaction rate was thermodynamically accelerated, thereby achieving synergistic PTT–CDT enhancement. The combination of mitochondrial localization and mild hyperthermia triggered an amplified local ROS burst. In vitro cellular experiments demonstrated that, under 660 nm laser irradiation at 0.5 W/cm 2 , a tumor cell killing rate of 75.89% was achieved through PDT/CDT/PTT trimodal synergistic effects, and cancer cell apoptosis was significantly enhanced. Consequently, this work provides a novel strategy for precision cancer therapy by co-localizing ROS generation and mild hyperthermia at the most vulnerable subcellular site.
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Food and Bioprocess Technology
·
2026
·Vol. 19
·期 6
·311
·
IF 5.6
Chang Haijun,Huang Yu,Wen Xin,Shi Yuanwei,Zhou Wenbin,Wu Li
This study investigated the effects of varying concentrations of purslane extract (PE) (0, 0.05, 0.10, 0.15, 0.20, and 0.40 mg mL −1 ) on the gelling properties of pork myofibrillar proteins (MPs) under a Fenton oxidation system, with 0.02 mg g −1 BHA as a positive control. Both BHA and PE alleviated the deterioration of gel properties induced by oxidation, as evidenced by suppressed cooking loss, improved water-holding capacity (WHC), and enhanced texture characteristics. Notably, PE at 0.20 mg mL −1 exhibited the most pronounced effects, leading to increased elasticity, cohesiveness, gumminess, and resilience, as well as maximal storage ( G ′) and loss ( G ″) modulus. LF-NMR analysis revealed that oxidation promoted the migration of water from immobilized to free states, a trend reversed by BHA and PE. The immobilized water content was significantly higher at 0.20 mg mL −1 PE than in the oxidized and BHA groups ( P < 0.05), indicating an improved gel network. Microstructural analysis further confirmed that the addition of 0.20 mg mL −1 PE under oxidative conditions significantly improved the three-dimensional network structure of MPs gels. However, further increasing the PE concentration to 0.40 mg mL −1 deteriorated all gel properties, highlighting a concentration-dependent regulatory effect. Therefore, optimizing PE concentration is critical for practical applications. These findings provide a theoretical basis for using PE as a natural antioxidant in gel-type meat products.
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JOURNAL OF POLYMER RESEARCH
·
2026
·Vol. 33
·期 5
·213
·
IF 3.5
Meng Chihan,Li Hongjiao,Feng Xiao,Zhao Yuanbo,Zhong Jiachun,Pu Zejun,Luo Yuxiu,Zhang Gaoping
In this paper, long carbon chain bio-based semi-aromatic polyamide PA9T, PA10T and PA12T were synthesized by using bio-based monomers nonenediamine, decamethylene diamine and dodecane diamine as raw materials. Materials studio software, universal testing machine, impact testing machine and other software and instruments were used, the physical and chemical properties of PA9T, PA10T and PA12T were studied in detail from both micro and macro perspectives. The results show that in the single-molecule model, PA12T exhibits the largest Poisson’s ratio, but the smallest bulk modulus, shear modulus, and Young’s modulus; PA9T shows the opposite trend, while PA10T is intermediate between the two, indicating that the less methylene in the single molecule model, the better the mechanical properties. From the macroscopic point of view, with the increase of methylene content, the tensile strength and bending strength decreased, while the elongation at break and the impact strength increased. The density also showed a gradual decreasing trend, and the oil absorption rate and water absorption rate gradually decreased. At the same time, with the increase of carbon chain, the solvent resistance is gradually enhanced, and the performance retention rate is gradually increased.
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Nature Communications
·
2026
·
IF 18.1
Zhang Weizhe,Wang Tao,Zhuo Yuhang,Li Shuang,Shi Jixin,Wei Haibing,Lin Weiran,Shi Yixiang,Wan Xinhua,Cai Ningsheng,Wang Bo
Anion exchange membrane water electrolyzers present a promising approach to cost-effective green H 2 generation, whereas integration of alkaline media and dry-cathode conditions intrinsically forbids adequate H 2 O/OH - conduction for efficient operation at high current densities. Herein, we develop a quinuclidinium-functionalized membrane possessing a modulated nano-porous architecture, and exploit its synergy with regulated configuration featuring an anode-to-cathode pressure gradient. By facilitating H 2 O permeation across interconnected hydrophilic nano-channels, a performance of 11.2 A·cm −2 at 2 V and 90 °C is realized using a NiFe anode, while sufficient membrane robustness and durability enable 2000 h operation at 1 A·cm −2 with suppressed decay of <1 μV·h −1 . The narrowed (1-2 nm) gas avenues coordinate with applied pressure gradient to mitigate H 2 crossover, improving adaptability to various static-dynamic scenarios. An encouraging levelized cost of H 2 of 1.8 $·kg −1 unveils the promise for up-scaled deployment, and this proposed membrane-condition collaboration advances to innovate next-generation energy technologies.
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产品关联
DOI
PubMed
高影响因子
ARCHIVES OF TOXICOLOGY
·
2026
·
IF 10.9
Zhao Yanjia,Yang Yi,Ding Zifang,Sun Yao,Zhang Mingyu,Li Weiwei,Liu Ting,Hu Zixia,Peng Ying,Zheng Jiang
Rosiglitazone (RGZ), a thiazolidinedione insulin sensitizer, is widely used in the treatment of type 2 diabetes mellitus. However, concerns regarding its dose-dependent hepatotoxicity have emerged, and the mechanisms of the toxic action remain elusive. This study aimed to investigate the mechanism by which an aldehyde metabolite generated through metabolic activation of RGZ forms covalent adduction with protein lysine residues and its contribution to hepatotoxicity. Using in vitro liver microsomal incubations coupled with LC–MS/MS, the reactive aldehyde metabolite and its corresponding lysine adduct were identified. It was verified that RGZ was primarily metabolized by CYP3A and CYP2E1 into an electrophilic aldehyde metabolite which subsequently underwent covalent bonding with the ε -amino group of lysine residues. The formation of the protein adduction was found to be associated with the hepatotoxicity of RGZ.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Luyao Xu,Jing Feng,Shihao Xu,Mingli Jin,Xianyue Bai,Hui Zhang,Minghui Zhu,Shengmin Lin,Jiaxing Song,Cuixia Lu
Multimodal targeted combination therapy that harnesses synergistic effects has emerged as a transformative paradigm in cancer therapy, progressively replacing traditional monotherapy. Herein, we report a tumor microenvironment (TME)-responsive multifunctional nanoplatform Cu-Ce6@DHA NPs (CCD NPs), which is self-assembled through the coordination of Cu²⁺, the antitumor drug dihydroartemisinin (DHA), and the photosensitizer chlorin e6 (Ce6). This nanoplatform enables the near-infrared-triggered combination of cuproptosis and ferroptosis for tumor treatment. Upon internalization by tumor cells, these nanoparticles undergo glutathione (GSH)-triggered disintegration, releasing their encapsulated payloads within the TME. The released Ce6 mediates potent photodynamic therapy (PDT) under laser irradiation, and DHA undergoes GSH-dependent activation to generate cytotoxic reactive oxygen species (ROS) and suppresses glutathione peroxidase 4 (GPX4), thereby amplifying ferroptotic cell death. Concurrently, the released copper ions deplete intracellular GSH and further inhibit GPX4, which exacerbates lipid peroxidation and promotes ferroptosis. Notably, the intracellular accumulation of copper ions disrupts mitochondrial metabolism by destabilizing iron-sulfur cluster (Fe-S) proteins and inducing oligomerization of lipoylated lipoylated dihydrolipoamide S-acetyltransferase (DLAT), ultimately triggering cuproptosis. Therefore, our findings establish a novel nanoplatform that simultaneously exploits metabolic vulnerabilities (via cuproptosis), redox imbalances (via ferroptosis), and photodynamic effects, providing a promising multimodal therapeutic strategy for cancer treatment.
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