Nature Communications
·
2023
·Vol. 14
·期 1
·1-11
·
IF 16.6
Pillai Hemanth Somarajan,Li Yi,Wang Shih-Han,Omidvar Noushin,Mu Qingmin,Achenie Luke E. K.,Abild-Pedersen Frank,Yang Juan,Wu Gang,Xin Hongliang
The electrochemical ammonia oxidation to dinitrogen as a means for energy and environmental applications is a key technology toward the realization of a sustainable nitrogen cycle. The state-of-the-art metal catalysts including Pt and its bimetallics with Ir show promising activity, albeit suffering from high overpotentials for appreciable current densities and the soaring price of precious metals. Herein, the immense design space of ternary Pt alloy nanostructures is explored by graph neural networks trained on ab initio data for concurrently predicting site reactivity, surface stability, and catalyst synthesizability descriptors. Among a few Ir-free candidates that emerge from the active learning workflow, Pt 3 Ru-M (M: Fe, Co, or Ni) alloys were successfully synthesized and experimentally verified to be more active toward ammonia oxidation than Pt, Pt 3 Ir, and Pt 3 Ru. More importantly, feature attribution analyses using the machine-learned representation of site motifs provide fundamental insights into chemical bonding at metal surfaces and shed light on design strategies for high-performance catalytic systems beyond the d -band center metric of binding sites.
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Journal of Food Measurement and Characterization
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2023
·Vol. 17
·期 3
·2850-2857
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IF 3.4
Yang Xiao-Shuai,Wang Lu-Liang,Zhao Jie,Ji Shi-Lei,Liu Quan-Wen,Peng Xin-Yan,Hu Zhen-Hua,Liu Yu-Shen,Wang Ping
Facile fabrication of copper nanoclusters (CuNCs) with excellent fluorescence is desirable, but remains challenging and scarce for sensing applications in food. In this work, albumin egg (AE) stabilized CuNCs were prepared by a self-assembly templated method. The obtained fluorescent AE-CuNCs exhibited a strong emission at 412 nm upon excitation at 325 nm. Based on the absorption competition quenching mechanism, the AE-CuNCs fluorescent probe for Fe 3+ was developed. The probe had good linearity (0.10–1000 µM) with R 2 (0.986), low limits of detection (30 nM) and good selectivity. Furthermore, the iron content in noodles, biscuits, light soy sauce and bean sprouts was successfully determined by the fluorescent probe. Graphical Abstract
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Scientific Reports
·
2023
·Vol. 13
·期 1
·1-10
·
IF 4.6
Zhao Xiaojiong,Tian Min,Wei Ruichao,Jiang Saihua
To improve flame retardancy of wood, a novel high-water-retention and self-healing polyvinyl alcohol/phytic acid/MXene hydrogel coating was developed through facile one-pot heating and freeze–thaw cycle methods, and then painted on wood surface. The coating exhibit excellent self-healing property and significantly enhanced water-retention property (water content ≥ 90 wt%), due to the increased hydrogen bonds within the coating system with the presence of MXene nanosheets. Compared to pristine wood, the flame retardancy of coated wood is greatly improved, such as passed V0 rating in UL-94 test, increasing time to ignition (TTI, from 32 to 69 s), and decreased heat release rate and total heat release by 41.6% and 36.14%. The cooling effect and large thermal capacity of high-water-retention hydrogel, and physical barrier effects for flammable gas products, heat and oxygen by MXene nanosheets and the compact char layer formed during combustion play key roles in the flame retardancy enhancements of the wood. High thermal stability of MXene nanosheets is another beneficial factor. The detailed flame-retardant and self-healing mechanisms were proposed.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2023
·Vol. 107
·期 5
·1551-1562
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IF 5
Ye Jianbin,Li Jianqing,Wang Qiuhui,Wang Xiangjiang,Wang Shouan,Wang Honglin,Xu Jia
Abstract
In this study, loquat extract was selected as a promising substrate for bacterial cellulose (BC) production. A new BC-producing bacterial strain was isolated from residual loquat and identified as Komagataeibacter rhaeticus . BC production with different carbon sources and with loquat extract was investigated. Among all tested carbon sources, glucose was demonstrated to be the best substrate for BC production by K. rhaeticus , with up to 7.89 g/L dry BC obtained under the optimal initial pH (5.5) and temperature (28 °C) with 10 days of fermentation. The total sugar and individual sugars were investigated in different loquat extracts, in which fructose, glucose, and sucrose were the three main sugars. When loquat extract was prepared with a solid‒liquid (S-L) ratio of 2:1, the concentrations of glucose, fructose, and sucrose were 7.91 g/L, 9.31 g/L, and 2.84 g/L, respectively. The BC production obtained from loquat extract was higher than that of other carbon sources except glucose, and 6.69 g/L dry BC was obtained from loquat extract with an S-L ratio of 2:1. After BC production, all sugars substantially decreased, with the utilization of glucose, fructose, and sucrose reaching 93.9%, 87.9%, and 100%, respectively. These results suggested that the different sugars in loquat extract were all carbon sources participating in BC production by K. rhaeticus . Structural and physicochemical properties were investigated by SEM, TGA, XRD, and FT-IR spectroscopy. The results showed that the structural, chemical group, and water holding capacity of BC obtained from loquat extract were similar to those of BC obtained from glucose, but the crystallinity and thermal stability of BC were higher than those of BC from mannose and lactose but lower than those of BC from glucose and fructose. Key points A new BC-producing strain was isolated and identified as Komagataeibacter rhaeticus.
Loquat extract is an alternative substrate for BC production.
The BC obtained from loquat extract owns advanced physicochemical properties . Graphical Abstract
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JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 6
·2764-2781
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IF 4.5
Zhang Xiangke,Chen Ming,Kan Yuping,Dong Yumeng,Zhang Xinyu,Wang Xueying,Su Hui,Xu Shiyi,Yan Xueying
A series of antitumor therapies such as chemotherapy, photothermal therapy, and photodynamic therapy have certain limitations when applied alone. Herein, the chemotherapy drug doxorubicin (DOX) and photosensitizer new indocyanine green (IR820) were loaded into mesoporous silica nanoparticles (MSNs), and it was wrapped by manganese dioxide (MnO 2 ) on the surface. Optimization of the synthesis conditions by Box–Behnken design (BBD) generated DOX-IR820@MSN–MnO 2 nanoparticles with a high loading capacity. The experiments suggested that the nanocomposites had tumor microenvironment response characteristics and excellent photodynamic and photothermal properties. It can significantly regulate the level of glutathione and reduce the antioxidant effect of cells to enhance the photodynamic effect. NIR laser irradiation increases the temperature and accelerates drug release. The relative cell viability in the multi-modal combination therapy group almost disappeared at 17.20%. Combined tumor microenvironment response and multi-modal therapy show unique advantages in inhibiting tumor cell proliferation and have promising applications and development potential in cancer treatment. Graphical Abstract
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NEUROCHEMICAL RESEARCH
·
2022
·Vol. 48
·期 5
·1424-1437
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IF 4.414
Zhao Yanling,Yan Han,Liang Xue,Zhang Zhenyu,Wang Xuan,Shi Nianwei,Bian Weihong,Di Qing,Huang He
Astrocyte activation is key in neurodegenerative diseases. Hydrogen sulfide (H 2 S) exhibits neuroprotective effects on astrocytes, although the underlying molecular mechanism remains unclear. Here, we explored the effects of H 2 S on lipopolysaccharide (LPS)-induced astrocyte activation and astrocyte-mediated neuroinflammation. After inducing primary astrocytes via LPS exposure, H 2 S levels were altered. The generation and secretion of inflammatory mediators by astrocytes and their interrelation with P-glycoprotein (P-gp), an important transporter belonging to the ABC transporter family, were assessed. Activated astrocytes showed upregulated glial fibrillary acidic protein ( GFAP ) mRNA expression, and significantly increased proinflammatory factor mRNA/protein expression and release. The secretory capacity of astrocytes was reduced, with significantly decreased proinflammatory factor levels in culture supernatant after P-gp inhibitor verapamil pretreatment. The increase in the intracellular H 2 S level inhibited LPS-induced GFAP expression and P65 nuclear entry in astrocytes. mRNA expression and release of proinflammatory factors were reduced significantly, with no significant changes in cytoplasmic protein expression. S-sulfhydration levels increased significantly with the increased concentration of sodium hydrosulfide or S-adenosyl- l -methionine addition, with only moderate changes in astrocyte P-gp expression. H 2 S regulates NF-κB activation, leads to S-sulfhydration of P-gp, and inhibits the biosynthesis and secretion of proinflammatory factors by astrocytes. The regulatory effects of H 2 S on astrocytes may have clinical value for exploring new therapeutic strategies against neurodegenerative diseases.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2022
·Vol. 415
·期 18
·4265-4275
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IF 4.478
Wang Tingting,Chen Yihui,Hou Chunyan,Qiao Xiaoqiang
A novel type of PEG-modified halloysite was prepared and used as a hydrophilic interaction and cation exchange mixed-mode sorbent for solid-phase extraction of biogenic amines in fish samples. The eluates were analyzed by high-performance liquid chromatography-ultraviolet detection after the derivatization with benzoyl chloride. The developed sorbent was characterized by scanning electron microscopy, infrared spectroscopy, X-ray diffraction, zeta potential analyzer, and thermo-gravimetric analysis. After the optimization of various parameters influencing the extraction efficiency, the PEG-modified halloysite-based SPE method was evaluated. The adsorption capacities of putrescine, spermine, phenethylamine, and histamine were as high as 9.3, 8.5, 5.7, and 5.6 mg g −1 , respectively. Satisfactory reproducibility of sorbent preparation was obtained with within-batch and batch-to-batch relative standard deviations (RSDs) lower than 3.9% and 8.6%, respectively. The biogenic amine spiking recoveries in fish samples ranged from 84.3 to 105.5% with good RSDs lower than 7.8%. Intra-day and inter-day precision, expressed as RSDs, were better than 8.8%. The limits of detection of histamine, putrescine, phenethylamine, and spermine were 9.4, 1.9, 0.5, and 0.9 μg L −1 , respectively. This work provides a new hydrophilic interaction and cation exchange mixed-mode sorbent and is successfully applied to the extraction of trace biogenic amines from fish samples. Graphical Abstract
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JOURNAL OF APPLIED ELECTROCHEMISTRY
·
2022
·Vol. 53
·期 3
·547-557
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IF 2.925
Hu Kaiyue,Ren Xinxin,Qin Lingxia,Guo Zhiyong,Wu Di,Wang Sui,Hu Yufang
The purpose of this study was to achieve a specific unlocking of the butterfly effect: nanointerface-based electrochemical biosensing of adenosine triphosphate (ATP) and alkaline phosphatase (ALP). Based on the Faraday-cage concept reported first by our group, we built a new outer Helmholtz plane (OHP)-based electrochemical biosensor by using an unique nanocomposite involving three-dimensional graphene-Au nanoparticles (3D-GO-AuNPs), tetrahedral DNA nanostructures (TDNs), and separated ATP aptamers, in which methylene blue (MB) was employed as the electrochemical signal output. In this process, the prepared nanocomposites were attached favorably onto the TDN substrate electrode surface due to the interaction of ATP and its aptamer, creating a better OHP of the electrode owing to its large enough specific surface area; then a detection limit of 0.25 pM was calculated by 3 δ /slope. Whereas, the hydrolysis for ATP of ALP can hinder this binding process, therefore, the biosensor could be indirectly applied for ALP analysis with a detection limit of 0.21 mU/L (3 δ /slope). Since some small changes of the two targets will set off a whole series of changes in system, the OHP-extended biosensor provides a superior electrochemical platform for complex biological processes with causal relationships in clinical diagnosis and drug development, similar to the butterfly effect. Graphical abstract
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Human Cell
·
2022
·Vol. 36
·期 1
·312-328
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IF 4.374
Long Jianwu,Liu Longfei,Yang Xuefeng,Zhou Xiaojun,Lu Xianzhou,Qin Lei
Emerging evidence has uncovered that noncoding RNAs (ncRNAs) contribute to the development of hepatocellular carcinoma (HCC). Nevertheless, the functions of the majority of long ncRNAs (lncRNAs) in HCC are unknown. Here, we intend to probe the function of lncRNA NUTM2A-AS1 in the evolvement of HCC and the related mechanism. Expression levels of lncRNA NUTM2A-AS1, miR-186-5p and KLF7 mRNA in HCC tissues and adjacent non-tumor tissues were monitored. Gain- or loss-of-function assays were utilized to investigate the biological functions of lncRNA NUTM2A-AS1, miR-186-5p and KLF7 in HCC cell lines (including HCCLM3 and Huh7). Western blot was implemented for the detection of the epithelial–mesenchymal transition (EMT)-related proteins (including E-cadherin, Vimentin and Snail), KLF7, Wnt, β-catenin, and stemness-related proteins (Nanog, OCT4, YKL40, and CD133). Furthermore, the targeted associations between lncRNA NUTM2A-AS1, miR-186-5p, and KLF7 were verified by bioinformatics analysis, dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. As a result, lncRNA NUTM2A-AS1 and KLF7 profiles were heightened in the HCC tissues versus adjacent normal tissues, while miR-186-5p had the opposite expression tendency. Up-regulation of lncRNA NUTM2A-AS1 was related to tumor size, advanced tumor stage, and lymph node metastasis of HCC patients. Functionally, overexpression of lncRNA NUTM2A-AS1 heightened HCC cells’ growth, invasion, EMT, and stemness and repressed their apoptosis by activating the Wnt/β-catenin pathway. In contrast, up-regulation of miR-186-5p or inhibition of KLF7 had reverse effects. In vivo, lncRNA NUTM2A-AS1 overexpression facilitated tumor growth and EMT, accompanied by declined miR-186-5p levels and enhanced KLF7 expression. The mechanistic studies revealed that miR-186-5p served as a common target of lncRNA NUTM2A-AS1 and KLF7. As hinted by the rescue experiments, NUTM2A-AS1 partly abated miR-186-5p-mediated anti-tumor effects in HCC cells, whereas KLF7 knockdown reversed the promotive effects of NUTM2A-AS1. LncRNA NUTM2A-AS1 accelerated the evolution of HCC by up-regulating the KLF7/Wnt/beta-catenin pathway through sponging miR-186-5p.
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CELLULOSE
·
2022
·Vol. 30
·期 2
·1087-1102
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IF 6.123
Li Chunyu,Hou Zehua,Li Pan,Zhang Guomin,Bai Liangjiu,Wang Wenxiang,Chen Hou,Yang Huawei,Yang Lixia
Cellulose nanocrystals (CNCs) have been widely used in the surface modification and construction of nanocomposites due to their excellent surface activity and high tensile strength. In this manuscript, nanocomposite hydrogels were prepared by implanting poly(glycidyl methacrylate)-modified CNCs (CNCs@PGMA) and biomass phytic acid (PA). After extracting CNCs from microcrystalline cellulose, PGMA was successfully grafted on the surface of CNCs by surface-initiated Activator Generated by Electron Transfer Atom Transfer Radical Polymerization (SI-AGET ATRP). With the assistance of PA, the reversible hydrogen bonding interaction between the internal structures of the matrix was promoted to construct the CNCs@PGMA/PA self-healing nanocomposite hydrogels. The obtained hydrogels by using CNCs@PGMA and PA as additives have excellent mechanical properties (tensile strength of 1.39 MPa) and self-healing ability (91.4% after 6 h). This study develops a facile method for the preparation of nanocomposite hydrogels with excellent self-healing properties. Graphical abstract
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-11
·
IF 17.694
Xiao Yang,Ribe Neil M.,Zhang Yage,Pan Yi,Cao Yang,Shum Ho Cheung
The solutal Marangoni effect is attracting increasing interest because of its fundamental role in many isothermal directional transport processes in fluids, including the Marangoni-driven spreading on liquid surfaces or Marangoni convection within a liquid. Here we report a type of continuous Marangoni transport process resulting from Marangoni-driven spreading and Marangoni convection in an aqueous two-phase system. The interaction between a salt (CaCl 2 ) and an anionic surfactant (sodium dodecylbenzenesulfonate) generates surface tension gradients, which drive the transport process. This Marangoni transport consists of the upward transfer of a filament from a droplet located at the bottom of a bulk solution, coiling of the filament near the surface, and formation of Fermat’s spiral patterns on the surface. The bottom-up coiling of the filament, driven by Marangoni convection, may inspire automatic fiber fabrication.
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AAPS PHARMSCITECH
·
2022
·Vol. 24
·期 1
·1-14
·
IF 4.026
Wang Xiaoling,Du Jiangyue,Zhou Feng,Ye Qing,Chen Ying,Sun Dujuan,Chen Haimin,Lv Yuanyuan,Sun Xiaoyi
Adequate delivery of therapeutic agents to their intended molecular targets is crucial in tumor therapy. Versatile drug carriers need to overcome the challenges coming from the systemic circulation, membrane barriers, and endo-lysosomal degradation. Herein, hyaluronic acid-conjugated polydopamine (HA-PDA)-shelled mesoporous silica nanoparticles encapsulated with doxorubicin (MSNs-DOX) were successfully fabricated for targeted tumor therapy. Compared with reported studies focusing on the pH-sensitive release in tumors, we especially revealed the significant role of lysosomal release in DOX nuclear accumulation. After active targeting and CD44-mediated endocytosis in tumor cells, the PDA layer of the nanoparticles would be peeled off to trigger drug release owing to MSNs gatekeeper in acidic lysosomes. Subsequently, DOX molecules passively diffused into nuclei. The intracellular DOX transportation was evidenced by DOX accumulation in nuclei, lysosomal location of nanoparticles, and lysosome acidification inhibition test. After discharging of the cargoes from nanoparticles, PDA shells from residual nanoparticles were able to produce localized hyperthermia under NIR irradiation entrapped in lysosomes, inducing synergistic chemo-photothermal effect. Under NIR treatment, HA-PDA@MSNs-DOX presented a prominent tumor inhibition rate without obvious side effects. This study indicated the potent nuclear delivery and synergetic chemo-photothermal therapy achieved by HA-PDA-shelled MSNs. Graphical Abstract
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危险品化学品经营许可证（带存储）