Probiotics and Antimicrobial Proteins
·
2019
·Vol. 12
·期 1
·32-38
·
IF 2.962
Zhang Jianming,Han Xue,Zhang Lanwei,Yi Huaxi,Chen Shiwei,Gong Pimin
The effects of fructose on plantaricin Q7 biosynthesis in Lactobacillus plantarum ( L. plantarum ) Q7 were investigated. Fructose induced and accelerated plantaricin Q7 production in the early stages of biosynthesis. Two-dimensional electrophoresis and quantitative real-time reverse transcriptase PCR (qRT-PCR) revealed that shock-related proteins were overexpressed in L. plantarum grown in MRS-fructose compared with cultures grown in MRS broth. A preheat-shock (PHS) treatment was used to overexpress shock-related proteins to enhance plantaricin Q7 production. The final plantaricin Q7 level reached 2873.08 IU/mL following PHS treatment, which was 23.36% higher than that obtained using a control strategy without PHS treatment. qRT-PCR analyses indicated that only groL was induced (by 10-fold) following PHS treatment. Evidently, groL plays a critical role in plantaricin Q7 biosynthesis. These results demonstrate that PHS treatment is an efficient approach to improving plantaricin Q7 production. The information obtained in this study may increase the amount of efficient antimicrobial peptide production using L. plantarum .
✓ 已验证
DOI
PubMed
高影响因子
Frontiers of Chemical Science and Engineering
·
2023
·Vol. 17
·期 4
·404-414
·
IF 4.5
Cheng Junjun,Zhao Yitao,Xu Guohao,Zhang Peng,Zhu Xuedong,Yang Fan
Alkylation of benzene with carbon dioxide and hydrogen to produce toluene and xylene could increase the added-value of surplus benzene as well as relieve environmental problems like green-house effect. In this work, the alkylation benzene with carbon dioxide and hydrogen reaction was proceeded by using the mixture of zinc-zirconium oxide and HZSM-5 as bifunctional catalyst. The equivalent of Zn/Zr = 1 displays the best catalytic performance at 425 °C and 3.0 MPa, and benzene conversion reaches 42.9% with a selectivity of 90% towards toluene and xylene. Moreover, the carbon dioxide conversion achieves 23.3% and the carbon monoxide selectivity is lower than 35%, indicating that more than 50% carbon dioxide has been effectively incorporated into the target product, which is the best result as far as we know. Combined with characterizations, it indicated that the Zn and Zr formed a solid solution under specific conditions (Zn/Zr = 1). The as-formed solid solution not only possesses a high surface area but also provides a large amount of oxygen vacancies. Additionally, the bifunctional catalyst has excellent stabilities that could keep operating without deactivation for at least 80 h. This work provides promising industrial applications for the upgrading of aromatics.
✓ 已验证
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 5
·1881-1890
·
IF 4.478
Wu Yike,Liu Yuanyuan,Shang Zhengjun,Liu Xin,Xu Yong,Liu Wenlan
Aberrant protein N-glycosylation is one of the hallmarks of malignancy, while its role in oral cancer (OC) progression is still poorly understood making it a perfect biomarker for early-stage cancer. In this work, we describe a MALDI-MS-based N-glycomic analysis with multivariate data analysis approach for exploration and classification of the patients with OC. Various statistical analyses were used to determine differential N-glycome profiles. There were 8 glycan structures significantly increased ( p < 0.05) in all OC-derived microvesicles (MVs). Importantly, the increases in these glycans were significantly greater in the early stage of OC than those in the advanced stage of OC. Additionally, N-glycomic profiles from the serum were also performed, demonstrating a significant change of 11 glycan structures ( p < 0.05). Our data highlighted the effectiveness approach of MALDI-MS-based MV N-glycomic profiling on OC screening, and presented strong potential for further identifying targeted glycoproteomics signatures of the cancer.
✓ 已验证
DOI
PubMed
高影响因子
Frontiers of Materials Science
·
2021
·Vol. 15
·期 3
·406-415
·
IF 2.765
He Xia,Liu Qingchun,Zhou Ying,Chen Zhan,Zhu Chenlu,Jin Wanhui
Wearable gas sensors can improve early warning provision for workers in special worksites and can also be used as flexible electronic platforms. Here, the flexible multifunctional gas sensor was prepared by grafting graphene oxide (GO)-Ag onto cotton fabric after swelling. The maximum bacterial inhibition rate of GO-150/cotton fabric was 95.6% for E. coli and 87.6% for S. aureus , while retaining the original high moisture permeability of cotton fabric. So GO/cotton fabric can resist the multiplication of bacteria. At the same time, GO can greatly improve the UV protection performance of cotton fabric used in garments. With increase of the GO concentration, the UV protection ability of composite fabric is enhanced. Finally, GO-Ag/cotton fabric sensors had stable NH 3 gas-sensitive properties and good washing stability. In conclusion, these cotton fabric sensors with antibacterial properties, UV resistance and highly sensitive gas-sensitive properties have potential applications in wearable early warning devices and textile products.
✓ 已验证
DOI
高影响因子
TRANSACTIONS OF TIANJIN UNIVERSITY
·
2020
·Vol. 26
·期 6
·470-478
·
IF 0
Xu Rongjie,Xu Hua,Ning Shangbo,Zhang Qiqi,Yang Zhongshan,Ye Jinhua
Visible light photocatalytic CO 2 conversion is a promising solution to global warming and energy shortage. Herein, we build a well-designed bridge-like nanostructure, that is, the phosphonated Ru complex (RuP) light absorber–TiO 2 bridge–Cu catalyst. In this nanostructure, brookite TiO 2 serving as a bridge is spatially connected to the RuP and Cu on each of its sides and could thus physically separate the photoexcited holes and electrons over the RuP and Cu, respectively. Given its effective charge separation, this RuP–TiO 2 –Cu assembly exhibits superior CO 2 photoreduction activity relative to RuP–SiO 2 –Cu under visible light irradiation ( λ > 420 nm). The catalytic activity is further optimized by adopting brookite TiO 2 with various electronic band structures. Results reveal the rapid movement of electrons from the RuP through the conduction band of TiO 2 and finally to the Cu surface. This property is crucial in CO 2 photoreduction activity.
✓ 已验证
DOI
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 10
·4091-4098
·
IF 4.58
Liu Haochi,Chen Ligang,Ding Jie
The authors describe an efficient method for the extraction of tetracycline antibiotics (TCs) from water samples by using a new kind of core-shell adsorbent. It consists of a magnetite (Fe 3 O 4 ) core and a metal-organic framework (type ZIF-8) as the shell. The sorbent has a large specific surface and superparamagnetic response. The sorbent was characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, nitrogen adsorption, and X-ray diffraction. The adsorption isotherms, dynamic adsorption, and mechanisms of interactions between tetracycline and sorbent were studied. Various parameters affecting the extraction efficiency were optimized. Following desorption of the TCs with methanol (containing 1% of acetic acid), they were quantified by using ultra-high performance liquid chromatography combined with mass spectrometry. The method has a linear range that extends from 0.5 to 50 ng·L −1 , and the limits of detection for tetracycline, chlortetracycline, and oxytetracycline are 0.125, 0.122 and 0.143 ng·L −1 , respectively. The relative standard deviations of intra-day and inter-day assays range from 3.3% to 8.5%, and from 2.8% to 8.3%, respectively. The recoveries of TCs (at spiking levels of 0.5, 5.0 and 50 ng·L −1 ) are in the range between 78.3% and 93.7%. The method was successfully applied to the determination of TCs in spiked river water, fish pond water, and livestock wastewater.
✓ 已验证
DOI
高影响因子
Metabolomics
·
2017
·Vol. 13
·期 12
·1-14
·
IF 3.692
Tang Tian,Gao Qun,Lin Hua,Biville Francis,Xiong Jingyuan,Pei Xiaofang,Zheng Bo,Zou Xiaoli,Wang Chuan
Introduction ClpXP protease is an important proteolytic system in Salmonella enterica serovar typhimurium ( S. typhimurium ). Inactivation of ClpXP by deletion of clpP resulted in overproduction of RpoS and a growth defect phenotype. Only one report has indicated that deleting rpoS can restore the growth of a S. typhimurium clpP mutant to the wild-type level. Whether overproduction of RpoS is responsible for the growth deficiency resulting from clpP disruption and how ClpXP affects the cell metabolism of S. typhimurium remain to be elucidated. Objectives The aim of this study is to investigate the effect of ClpXP on cell metabolism of S. typhimurium and explore the possible co-effect of RpoS associated with ClpXP in cell metabolism. Method We constructed a clpP rpoS double deletion mutant TT-19 (Δ clpP Δ rpoS TT-1) using a two-step phage transduction technique. We then compared the metabolite fingerprints of Salmonella rpoS deletion mutant TT-14 (Δ rpoS TT-1), clpP deletion mutant TT-16 (Δ clpP TT-1), and clpP rpoS double deletion mutant TT-19 (Δ clpP Δ rpoS TT-1) with those of the wild-type strain TT-1 by using gas chromatography coupled with mass spectrometry (GC–MS). Results Deletion of rpoS recovered only a part of the growth of Salmonella clpP mutant. Further metabolome analysis indicated that clpP disruption changed the levels of 16 extra- and 19 intracellular substances, while the extracellular concentrations of 4 compounds (serine, l -5-oxoproline, l -glutamic acid, and l -tryptophan) and intracellular concentrations of 10 compounds ( l -isoleucine, glycine, serine, l -methionine, l -phenylalanine, malic acid, citric acid, urea, putrescine, and 6-hydroxypurine) returned to their wild-type levels when rpoS was also deleted. Conclusion ClpXP affects the cell metabolism of S. typhimurium partially in an RpoS-dependent manner.
✓ 已验证
DOI
高影响因子
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
·
2022
·Vol. 27
·期 3
·443-450
·
IF 3.386
Yang Rong-ling,Chen Xi,Song Yu-ye,Zhu Qian-lin,Bilal Muhammad,Wang Yu,Tong Zheng,Wu Ting-ting,Wang Zhao-yu,Luo Hong-zhen,Zhao Xiang-jie,He Ting-ting
Tyrosinase inhibitors are clinically effective for treating some dermatological disorders related to melanin hyperpigmentation. Accordingly, the discovery and development of tyrosinase inhibitors have great value in the pharmaceutical and cosmetic industry. Here, a novel tyrosinase inhibitor, 6′-O-cinnamoyl-helicid (helicid cinnamylate) was successfully synthesized by a simple and effective biocatalytic approach with Aspergillus oryzae cells. Investigation of the effects of several key variables on helicid cinnamylate synthesis found that the reaction conversion, reaction rate and regioselectivity reached 99%, 9.40 mM/h and > 99%, respectively, at the optimal conditions with anhydrous acetone as the solvent, whole-cell concentration of 40 mg/mL, and the molar ratio of vinyl cinnamate to helicid of 10 at 45°C. The whole-cells retained 68.87% of its initial activity after reusing for seven batches, indicating a potent application potential in non-aqueous biocatalytic systems. It was worth noting that helicid cinnamylate demonstrated a more potent tyrosinase inhibitory activity with an IC 50 value of 3.55 mM than helicid (IC 50 = 4.48 mM) and arbutin (IC 50 = 5.48 mM), which suggest that helicid cinnamylate could be developed as a more potential tyrosinase inhibitor. In conclusion, this study provides a novel whole-cell catalytic approach for the synthesis of helicid cinnamylate and insight into its application as a tyrosinase inhibitor.
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 11
·1-8
·
IF 5.479
Wang Shuangling,Yan Han,Wang Yalin,Wang Na,Lin Yulong,Li Meng
The peroxidase-like activity of hollow Prussian Blue nanocubes (hPBNCs) is used, in combination with the enzyme alcohol oxidase (AOx), in a colorimetric ethanol assay. Different from other nanozymes, the large cavity structure of the hPBNCs provides a larger surface and more binding sites for AOx to be bound on their surface or in the pores. This extremely enhances the sensitivity of the assay system. In the presence of ethanol, AOx is capable of catalyzing the oxidation of alcohols to aldehydes, accompanied by the generation of hydrogen peroxide (H 2 O 2 ). The hPBNCs act as peroxidase mimics and then can catalyze the oxidation of 3,3′5,5′-tetramethylbenzidine (TMB) by H 2 O 2 , resulting in a color change of the solution from colorless to blue with a strong absorption at 652 nm. The lower detection limit for ethanol is 1.41 μg∙mL −1 . Due to the high catalytic activity of hPBNCs in weakly acidic and neutral solutions, the system was successfully applied to the determination of ethanol in mice blood. This is critically important for studying the alcohol consumption and monitoring the ethanol toxicokinetics.
Schematic representation of hollow Prussian Blue nanocubes (hPBNCs) used as both a peroxidase mimetic and as a carrier for alcohol oxidase. Utilizing hPBNCs along with the ethanol conversion enzyme, a sensitive colorimetric assay for ethanol was developed and applied to blood samples with satisfactory results.
✓ 已验证
DOI
PubMed
高影响因子
TRANSACTIONS OF TIANJIN UNIVERSITY
·
2018
·Vol. 24
·期 6
·538-546
·
IF 0
Qu Hongmei,Zhang Xin,Chen Xu,Zhang Hui,Men Yican,Li Junqiu
In this study, a new zirconium-mediated cycloaddition for preparing dibenzosilole derivatives was developed using silicon-bridged diynes and electron-withdrawing alkynes as starting materials. The preparation of silicon-bridged diynes from 1-bromide-2-iodobenzene, terminal alkynes, and dimethyldichlorosilane was also studied. Unlike in the previous synthesis methods, much higher yields of electron-withdrawing group-substituted dibenzosilole derivatives were obtained. In addition, a new synthesis strategy for preparing benzonaphthosilole derivatives using internal alkynes, 1,4-dibromobenzene, and electron-withdrawing alkynes as starting materials is proposed. Compared with previous methods, alkyl, phenyl, and electron-withdrawing groups can be successfully introduced onto aromatic rings, and the positions of these substituents can be easily controlled. The cycloaddition reactions for dibenzosilole and benzonaphthosilole derivatives are highly efficient one-pot processes, and the raw materials are available and easily prepared. Using these new methods, a series of novel multi-substituted dibenzonsilole and benzonaphthosilole derivatives were obtained effectively.
✓ 已验证
DOI
JOURNAL OF MATERIALS SCIENCE
·
2015
·Vol. 50
·期 18
·6032-6040
·
IF 2.371
Li Yuda,Wang Xunchang,Cheng Chang,Xiong Zichao,Shu Ge,Wang Feng
Pyrene-based nanotweezers 1 consisting of two pyrenes and amino-substituted carbazole in between have been successfully synthesized via Suzuki–Miyaura coupling reaction. The supramolecular complex formation of nanotweezers 1 with single-walled carbon nanotubes (SWNTs) was studied, and it was found that nanotweezers 1 exhibited much higher the SWNTs extraction ability as compared to the analogous dipyrene nanotweezers 2 and 3 with soluble alkyl-substituents. This enhanced extraction ability of 1 can be attributed to the formation of more stable SWNT complexes in methanol. On the basis of photoluminescence excitation and UV–Vis-NIR absorption of 76-CoMoCAT SWNTs before and after the extraction, nanotweezers 1 were found to show high selectivity towards (8,4)-, (7,6)-, (9,4)-, and (8,6)-SWNTs with diameter ranging from 0.84 to 0.97 nm.
✓ 已验证
DOI
高影响因子
Science China-Chemistry
·
2015
·Vol. 59
·期 1
·114-121
·
IF 1.695
Zhao Wenping,Jin Yu,Zhang Wen
Two phase transition compounds, diethylammonium 1,5-naphthalenedisulfonate (1) and cyclohexylammonium 1,5-naphthalenedisulfonate (2), were screened from a series of organic salts based on 1,5-naphthalenedisulfonate. The phase transition behaviors were studied by differential scanning calorimetry, single-crystal X-ray analysis and dielectric measurements. Compounds 1 and 2 undergo phase transitions at about 202 and 148 K, respectively, accompanied by distinct dielectric changes. The origin of the phase transitions was ascribed to motional changes of the cations in the crystal lattices.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）