JOURNAL OF HAZARDOUS MATERIALS
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2022
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IF 14.224
Dongyun Liao,Guifang Liang,Yuyao Liu,Wenjun Yan,Yujing Guo,Wenting Liang,Chuan Dong,Lifang Fan
In this work, an efficient and novel photoelectrochemical (PEC) aptasensor for 2,3′,5,5′-tetrachlorobiphenyl (PCB72) was constructed based on [email protected] [email protected] quantum dots ( [email protected] QDs)-decorated TiO 2 nanotubes (TiO 2 NTs). [email protected] QDs were prepared by the combination of CdTe and CdS with a proper lattice mismatch. Due to their large band offsets, [email protected] QDs can reduce undesirable carrier recombination, significantly improving their charge separation efficiency. Then the synthesized [email protected] QDs were modified on TiO 2 NTs ( [email protected] QDs/TiO 2 NTs) through electrostatic adsorption method. The as-prepared composites exhibit a wide visible light absorption range, good PEC activity and high photoelectric conversion efficiency. Also, the PEC aptasensor prepared via the immobilization of anti-PCB72 aptamer on the composites exhibits outstanding analytical performance with high sensitivity and specificity for PCB72 under visible-light irradiation, achieving a detection limit as low as 0.03 ng/L. It was also applied to detect PCB72 in four different real environmental samples with satisfactory results.
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FOOD CHEMISTRY
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2022
·
IF 9.231
Yuhao Zhu,Chenming Dai,Danyu Lv,Yongguang Guan
Nonionic oil-in-water (O/W) nanoemulsion provides potential to stabilize hydrophobic bio-functional components in aqueous medium. Understanding safety of nanoemulsion droplets via investigating in vitro cell uptake and cellular substructural changes is important to achieve their practical applications. Herein, we developed a nonionic O/W nanoemulsion to stabilize representative bio-functional hydrophobic component of 9′- cis -bixin at pH 3–7 and ultraviolet (UV)-induced degradation at 365, 302, and 254 nm. In vitro cell uptake demonstrated that Caco-2 cells adequately enriched 9′- cis -bixin through fast uptake of nanoemulsion droplets within 15 min. However, excessive nanoemulsion droplets greatly decreased cell survival rate, which was due to the potential destruction of cellular substructures of mitochondria, nuclear membrane, and cell membrane. Lower nanoemulsion concentration provided no significant effects on Caco-2 cell survival. This work provided objective understanding on bio-functional component stability by nanoemulsion with in vitro safety evaluation.
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SCIENCE OF THE TOTAL ENVIRONMENT
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2022
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IF 10.753
Dan Zhou,Mengmeng Liang,Yonglian Xia,Chao Li,Mingzheng Huang,Shuming Peng,Yi Huang
Extracellular polymeric substances (EPS) are high-molecular polymers secreted by microbes and play essential roles in metallic biogeochemical cycling. Previous studies demonstrated the reducing capacity of the functional groups on EPS for metal reduction. However, the roles of different EPS components in vanadium speciation and their responsible reducing substances for vanadium reduction are still unknown. In this study, the EPS of Bacillus sp. PFYN01 was fractionated via ultrafiltration into six components with different kDa (EPS >100 , EPS 100 – 50 , EPS 50 – 30 , EPS 30 – 10 , EPS 10 – 3 , and EPS <3 ). Batch reduction experiments of the intact cells, EPS-free cells, the pristine and fractionated EPS with V 5+ were conducted and characterized. The results demonstrated that the extracellular reduction of V 5+ into V 4+ by EPS was the major reduction process. Among the functional groups in EPS, C=O/C-N of amide in protein/polypeptide and C O of carboxyl in fulvic acid-like substances might act as the reductants for V 5+ , while C O in polysaccharide molecules and P O in phosphodiester played a key role in the adsorption process. The intracellular reduction was via translocating V 5+ into the cells and releasing V 4+ by the intracellular reductases. The reducing capacity of the fractionated EPS followed a sequence of EPS <3 > EPS 10 – 3 > EPS 50 – 30 > EPS 100 – 50 > EPS 30 – 10 > EPS >100 . The small molecules of fulvic acid-like substances and amino acids were responsible for the high reducing capacity of EPS <3 . EPS >100 had the lowest reducing capacity due to its macromolecular structure decreasing the exposure of the reactive sites. In addition to reduction, those intermediate EPS components may also have supporting functions, such as connecting protein skeletons and increasing the specific surface area of EPS. Therefore, the diverse effects of the EPS components cannot be neglected in vanadium biogeochemical cycling.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Yarong Lv,Zhongxun Yu,Chenxi Li,Jianwei Zhou,Xujin Lv,Jia Chen,Ming Wei,Jun Liu,Xiaomin Yu,Ce Wang,Ping Hu,Yong Liu
Infection is a huge obstacle to wound healing. Thus, to enhance the healing of infected wounds, wound dressings that permit the dual delivery of antimicrobials and antioxidants are highly desirable. In this study, a series of gelatin-based nanofiber membranes with different curcumin contents were fabricated via solution electrospinning. The obtained membranes were characterized in terms of their morphologies, in addition to their physical, mechanical, and in vitro properties. The results showed that the membranes maintained an integrated morphology, excellent water absorption capability, satisfactory mechanical properties, and a high dissolution rate of curcumin. The addition of curcumin and borneol conferred the membranes the ability to inhibit Staphylococcus aureus and eliminate free radicals. Furthermore, cytocompatibility testing using the L929 cell line confirmed the excellent biocompatibility of the membranes. These gelatin-based nanofiber membranes loaded with curcumin and borneol can therefore be considered as promising materials for dressing wounds. Moreover, the use of biodegradable polymers and environmentally sustainable production techniques in this system render it suitable for the commercial manufacture of composite membranes.
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JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2022
·Vol. 169
·期 9
·097501
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IF 4.386
Dongmei Ren,Xingwen Wang,Chunpeng Leng,Wei Meng,Jingrui Zhang,Chao Han
4-Nitrophenol (4-NP) is a typical organic pollutant, thus it is urgent to detect it in environmental samples. This study rationally designed a novel electrode material of P-doping Fe/Fe3O4@C (P-Fe/Fe3O4@C) prepared with MIL-88 as precursor. P2h-Fe/Fe3O4@C-700 was synthesized by calcining MIL-88 at 700 °C, followed by annealing process with NaH2PO2 for 2 h. The P2h-Fe/Fe3O4@C-700 modified glassy carbon electrode (P2h-Fe/Fe3O4@C-700/GCE) showed a high electrocatalytic activity toward 4-NP reduction due to its active metal center, fine-tuned electronic structure by P-doping, as well as good electrical conductivity. It exhibited excellent sensing behaviors in 4-NP detection with rather high sensitivities of 1415.43 μA·mM−1·cm−2 and 424.63 μA·mM−1·cm−2 in the 4-NP concentration of 5 to 100 μM and 100 to 1200 μM, respectively, with a low detection limit of 0.462 μM. The P2h-Fe/Fe3O4@C-700/GCE still had good selectivity for 4-NP detection in the presence of common interferences. In addition, it was found that the current response towards 4-NP at P2h-Fe/Fe3O4@C-700/GCE retained more than 97% of its initial value after one week, indicating a good long-term stability. Furthermore, the P2h-Fe/Fe3O4@C-700/GCE exhibited a good reproducibility. The constructed P2h-Fe/Fe3O4@C-700/GCE achieved practical uses for the sensing of 4-NP in tap water with satisfactory recoveries.
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Acta Biomaterialia
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2022
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IF 10.633
Yifan Li,Qiangwei Liang,Liyue Zhou,Yongjing Cao,Jiayu Yang,Juan Li,Jinxia Liu,Jiawei Bi,Yanhua Liu
The signaling cascade between nuclear factor-kappa B (NF-κB) and hypoxia-inducible factor-1α (HIF-1α) can be activated by proinflammatory M1 macrophages in rheumatoid arthritis (RA), which produces reactive oxygen species (ROS) and enhances M1 macrophage polarization, thus aggravating the development of RA. Therefore, an ROS-responsive artesunate prodrug micellar nanosystem for co-delivery of dexamethasone (DEX/HA-TK-ART micelles , abbreviated as DEX/HTA) was developed for synergistic inhibition of the HIF-1α/NF-κB cascade to regulate ROS scavenging and macrophage repolarization in RA combination therapy. DEX/HTA micelles displayed prolonged circulation in blood and efficiently co-delivered ART&DEX in the inflamed joints of adjuvant-induced arthritis (AIA) rats; moreover, they were specifically recognized and internalized into M1 macrophages through CD44 receptor-mediated endocytosis . ROS-responsive co-released ART&DEX then exerted a synergistic action to efficiently perform ROS scavenging and repolarization of M1 to M2 macrophages by inhibition of the HIF-1α/NF-κB cascade. The intravenous administration of DEX/HTA micelles into AIA rat models significantly alleviated inflammatory cell infiltration and repaired cartilage injury in the joint. Collectively, our study highlights the therapeutic potential of DEX/HTA micelles for treating RA through synergistic inhibition of the HIF-1α/NF-κB signaling cascade to regulate ROS scavenging and macrophage repolarization. Statement of significance An ROS-responsive artesunate (ART) prodrug micellar nanosystem for co-delivering dexamethasone (DEX), abbreviated as DEX/HA-TK-ART micelle, was developed for synergistic cascade regulation of the HIF-1α/NF-κB pathway on ROS scavenging and macrophage repolarization in combination therapy for rheumatoid arthritis. The well-designed nanosystem showed prolonged circulation in blood and superior ART&DEX accumulation in the inflamed joints of AIA rats; moreover, the micelles were specifically internalized into M1 macrophages and co-released ART&DEX, subsequently leading to inhibition of the HIF-1α/NF-κB pathway for ROS scavenging and macrophage repolarization, thus generating synergistic anti-inflammatory effects in RAW 264.7 cells and AIA rats. The HIF-1α/NF-κB cascade regulation on ROS scavenging and macrophage repolarization based on ART&DEX combination with smart nanotechnology could serve as a promising approach for rheumatoid arthritis therapy.
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ACS Nano
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2022
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IF 18.027
Yihan Fu,Fei Ye,Xuwu Zhang,Yuchu He,Xiaoyu Li,Yongfu Tang,Jing Wang,Dawei Gao
Currently, one of the main reasons for the ineffectiveness of tumor treatment is that the abnormally high tumor interstitial pressure (TIP) hinders the delivery of drugs to the tumor center and promotes intratumoral cell survival and metastasis. Herein, we designed a “nanomotor” by in situ growth of Ag2S nanoparticles on the surface of ultrathin WS2 to fabricate Z-scheme photocatalytic drug AWS@M, which could rapidly enter tumors by splitting water in interstitial liquid to reduce TIP, along with O2 generation. Moreover, the O2 would be further converted to reactive oxygen species (ROS), accompanied by increased local temperature of tumors, and the combination of ROS with thermotherapy could eliminate the deep tumor cells. Therefore, the “nanomotor’’ could effectively reduce the TIP levels of cervical cancer and pancreatic cancer (degradation rates of 40.2% and 36.1%, respectively) under 660 nm laser irradiation, further enhance intratumor drug delivery, and inhibit tumor growth (inhibition ratio 95.83% and 87.61%, respectively), and the related mechanism in vivo was explored. This work achieves efficiently photocatalytic water-splitting in tumor interstitial fluid to reduce TIP by the nanomotor, which addresses the bottleneck problem of blocking of intratumor drug delivery, and provides a general strategy for effectively inhibiting tumor growth.
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Nano Today
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2022
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IF 18.962
Di Liu,Hongliang He,Fei Kong,Yixiang Cao,Fengchao Zang,Ming Ma,Ning Gu,Yu Zhang
Currently, multimodal synergistic nanoplatform emerges as an important cancer therapy paradigm, however, multimodal synergistic theranostic nanoplatform remains to be developed. Herein, using the mouse model of breast cancer, a versatile multimodal synergistic theranostic nanoplatform is rationally designed and synthesized for enhancing chemodynamic therapy (CDT), overcoming immunosuppression within tumor microenvironment , and inhibiting metastasis as well as tracking tumor. To fulfill our design, a composite Fe/Mn magnetic nanoparticle is first synthesized by loading with Fe 3 O 4 and BMS-202 (PD-L1 inhibitor) within a poly(lactide-co-glycolide)-based nanoparticle core, which are further modified with in situ synthesis of MnO 2 layer. Then, the composite metal-organic nanoparticle is coated with two targeting moieties hyaluronic acid (HA) and AMD3100 (CXCR4 antagonist), respectively, to achieve the multimodal synergistic theranostic nanoplatform ( [email protected] +AMD). With surface targeting modification, [email protected] +AMD exhibits enhanced tumor accumulation, where it effectively consumes endogenous glutathione to generate Mn 2+ allowing for the enhanced CDT effect, alleviates tumor hypoxia by O 2 generation and reverses the tumor immunosuppression. [email protected] +AMD blocks CXCR4 receptor on cancer cells, thus suppressing the CXCR4-mediated cancer metastasis and invasion. Additionally, [email protected] +AMD would synchronously be achieving tumor tracking by T 1 -T 2 dual-mode magnetic resonance imaging. Collectively, this strategy holds a novel multimodal synergistic theranostics for effective cancer management.
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FOOD HYDROCOLLOIDS
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2022
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IF 11.504
Yang Chen,Mengying Yao,Tianyi Yang,Yajing Fang,Dong Xiang,Weimin Zhang
Atmospheric pressure cold plasma (ACP) treatment, a non-thermal sterilization technology with great potential in coconut milk, can maximize the retention of the taste and flavour. However, it is unavoidable to have an impact on the coconut milk components. In this study, coconut globulin (CG), a component important for coconut milk stability, was selected to explore the effect of the ACP treatment on the structure and emulsifying properties of CG. After the ACP treatment, the structure of CG unfolded, which was the increase in random coils and reduction in intrinsic fluorescence intensity. In addition, the carbonyl and S–S bond content increased, while the free –SH content decreased. Compared with untreated CG (440.57 nm), the particle size of CG after the ACP treatment decreased, especially at 60 kV 60 s (207.63 nm) and 60 kV 90 s (204.20 nm). Simultaneously, poor modification and excessive modification of CG have similar surface hydrophobicity and interfacial tension to untreated CG. Notably, moderate modification (60 kV 60 s and 60 kV 90 s) resulted in the unfolding of CG molecules and the exposure of hydrophobic groups, increasing their adsorption at the oil-water interface. As expected, the emulsifying ability and emulsifying stability of CG were significantly improved after the ACP treatment at 60 kV 60 s and 60 kV 90 s. In conclusion, moderate ACP treatment could improve the emulsifying properties of CG by modifying its structure. This study provides new insights into the effect of ACP on food components during liquid food sterilization .
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PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
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2022
·Vol. 40
·期 1
·2200159
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IF 3.467
Yiliang Xie,Baowen Dong,Xuan Zhang,Jimin Fan,Jianying Lin,Bing Zhang,Zhihuan Zhao
In this study, biosynthetic Ag@AgCl plasma is used as a photosensitizer for the first time to construct a photothermal/photodynamic/chemotherapy and pH-responsive synergistic antitumor nanomedicine delivery system CS-Ag@AgCl/C. The Ag@AgCl and AgNPs synthesized by the extracts of alpine banana, C itrus reticulata , and Citrus sinensis are characterized. The Ag@AgCl NPs synthesized with banana extract have a regular crystal shape and a granular size of 12 nm, and they are more stable than AgNPs synthesized with the other two extracts. In addition, Ag@AgCl plasma used as a photosensitizer can reduce the dependence of CS-Ag@AgCl/C on oxygen in the photodynamic process. After coating with chitosan, though the photothermal conversion efficiency of CS-Ag@AgCl/C decreases from 44.06% to 29.51%, the pH response character of it increases, and the pH response plays an important role in drug release experiments. Under an acidic environment of pH = 5.0 phosphate-buffered saline buffer, the drug release rate of the samples increases significantly. In the synergistic antitumor experiment, the cell survival rate increases from 36.83% to 78.69% after chitosan-coated Ag@AgCl/C NPs. When CS-Ag@AgCl/C-DOX+808 nm is co-cultured with HeLa cells, the cell survival rate is only 14.89%, which indicates that the constructed plasma drug delivery system has excellent photothermal/photodynamic/chemotherapy combined treatment capability.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Haoyuan Li,Hongxiang Zhu,Zongyan Quan,Zhiping Chen,Lei Wang,Hui He
Gold nanoclusters with excellent fluorescence intensity, stability and biocompatibility have promising application prospects in the field of detection. Inspired by the growth behavior of sea squirts adhered to ropes, a novel bionic bifunctional cellulose nanocrystal (CNC)-based gold nanocluster probe was effectively constructed by directionally growing gold nanoclusters on CNCs modified with a specific DNA sequence . The constructed probe has excellent fluorescence properties and stability. The “ON or OFF” conversion mode of fluorescence was designed to detect different pathological markers in cells. In the “ON” mode, the probe readily combined with various sulfhydryl group containing markers (acetylcholinesterase, cysteine and glutathione) to produce fluorescence quenching and realize detection, the corresponding detection limits were as low as 0.015 mU/mL, 0.028 μM and 0.228 μM, respectively. Interestingly, the fluorescence of the probe could be regulated to the OFF mode via the assembly of the graphene oxide structure in accordance with Förster resonance energy transfer . The fluorescent probe with OFF mode could selectively recognize the carcinoembryonic antigen biomarker of malignant tumours via fluorescence enhancement, and its limit of detection was as low as 0.54 ng/mL. The as-developed novel strategy has positive significance for the early diagnosis of malignant tumours.
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Pharmaceutics
·
2022
·Vol. 14
·期 11
·2307
·
IF 6.525
Xueyan Gou,Fenglin Ran,Jinru Yang,Yanrong Ma,Xin’an Wu
Background: Organic anion transporter 1 (OAT1) and OAT3 have an overlapping spectrum of substrates such that one can exert a compensatory effect when the other is dysfunctional. As a result, the knockout of either OAT1 or OAT3 is not reflected in a change in the excretion of organic anionic substrates. To date, only the mOAT1 and mOAT3 individual knockout mouse models have been available. Methods: In this study, we successfully generated aSlc22a6/Slc22a8double-knockout (KO) rat model using CRISPR/Cas9 technology and evaluated its biological properties. Results: The double-knockout rat model did not expressionmRNAfor rOAT1 or rOAT3 in the kidneys. Consistently, the renal excretion ofp-aminohippuric acid (PAH), the classical substrate of OAT1/OAT3, was substantially decreased in theSlc22a6/Slc22a8double-knockout rats. The relativemRNAlevel ofSlco4c1was up-regulated in KO rats. No renal pathological phenotype was evident. The renal elimination of the organic anionic drug furosemide was nearly abolished in theSlc22a6/Slc22a8knockout rats, but elimination of the organic cationic drug metformin was hardly affected. Conclusions: These results demonstrate that this rat model is a useful tool for investigating the functions of OAT1/OAT3 in metabolic diseases, drug metabolism and pharmacokinetics, and OATs-mediated drug interactions.Keywords:OAT1/OAT3;CRISPR/Cas9;gene knockout;pharmacokinetic
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危险品化学品经营许可证（带存储）