INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Shiqing Zhang,Yifan Lin,Zhanhua Zhang,Xingyan Ou,Zhenbo Xu,Chengjuan Zou,Thanapop Soteyome,Lili Li,Xiaomei Lin,Yanrui Ye
Chitin, the second most abundant natural polysaccharide, is a renewable resource with great potential for producing functional oligosaccharides. In this study, we identified and characterized CtChi70, a glycoside hydrolase family 18 (GH18) exochitinase that selectively hydrolyzes chitin to produce N -acetyl chitobiose [(GlcNAc)₂] with exceptional purity (≥98 %). CtChi70 was heterologously expressed in Escherichia coli CtChi70 at a high yield of 228.2 ± 47.8 mg L −1 . The purified enzyme exhibited optimal activity at pH 5.0 and 45 °C, and demonstrated stability across a broad pH range (4.0–12.0). Time-course hydrolysis of colloidal chitin showed moderate catalytic efficiency but high product specificity, peaking at 24 h with minimal N -acetylglucosamine byproduct. The ability of CtChi70 to directly convert raw chitin powder to high-purity (GlcNAc)₂ suggests its potential for applications requiring pure chitooligosaccharides. These findings highlight CtChi70 as a promising biocatalyst for sustainable chitin valorization.
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高影响因子
JOURNAL OF THE INDIAN CHEMICAL SOCIETY
·
2025
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IF 3.4
Wei Shen,Zilong Yang,Xiaolin Guo,Hongxiao Jin,Wenxiang Chai,Hongliang Ge,Dingfeng Jin
Amino acid ionic liquids (AAILs) are potential green substitutes of aqueous amine solutions for carbon dioxide (CO 2 ) capture. In this study, we prepared three amino acid ionic liquids, constructed from tetrabutylphosphine hydroxide in combination with glycine, histidine, and arginine. We have conducted a comprehensive study on the CO 2 absorption capacity of structures containing single, double, and triple N–H groups. The solubility of CO 2 in [P 4444 ][Gly], [P 4444 ][His] and [P 4444 ][Arg] were measured experimentally over a temperature range of 30–110 °C and up to a pressure of 0.75 MPa. The results indicate that the solubility of carbon dioxide in amino acid ionic liquids decreases with increasing temperature, while it increases with increasing pressure. The optimal CO 2 absorption conditions of three amino acid ionic liquids were determined to be 50 °C, 0.75 MPa. Moreover, CO 2 absorption capacity of three amino acid ionic liquids followed the order: [P 4444 ][Arg] > [P 4444 ][His] > [P 4444 ][Gly]. Studies on the recycling and regeneration of amino acid ionic liquids verified their reusability in the process and confirmed that the reaction between AAILs and CO 2 could be reversed at 140 °C. Finally, the recycle capacity and CO 2 removal efficiency of [P 4444 ][Gly], [P 4444 ][His] and [P 4444 ][Arg] were evaluated.
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INTERNATIONAL IMMUNOPHARMACOLOGY
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2025
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IF 4.7
Sen Chen,Hui Lin,Haodi Gu,Xinrou Yu,Junjie Xiang,Lili Xu,Gang Ye,Yue Shan,Yun Wang
Introduction Sepsis-induced cardiomyopathy (SIC) is a rapidly advancing condition associated with a poor prognosis due to the lack of effective treatments. Neutrophil extracellular traps (NETs), however, act as a double-edged sword in the innate immune response during sepsis. N-formyl methionine (fMet) has been documented to induce NETs in inflammatory conditions, yet its clinical significance and biological function in SIC remain unclear. In this study, we investigated whether fMet induces excessive NETs, thereby promoting SIC. Methods and results Clinically, serum fMet levels were quantified using ELISA, revealing a significant elevation in patients with SIC compared to non-sepsis patients and healthy controls. The fMet levels were positively correlated with the NETs-related markers myeloperoxidase (MPO) and double-stranded DNA (dsDNA) in patients with SIC. Treatment with lipopolysaccharide and fMet increased NET formation in human neutrophils and upregulated the expression of formyl peptide receptor 1 (FPR1) and hypoxia-inducible factor 1-alpha (HIF-1α). Furthermore, bone marrow-derived neutrophils (BMDNs) were isolated from global FPR1 knockout mice, and FPR1 deficiency in BMDNs was found to suppress NETosis. The cecal ligation and puncture (CLP) model was employed to induce SIC in mice and we found knockout of FPR1 improved outcomes in CLP mice, as evidenced by survival benefit, increased cardiac function, attenuated cytokine storm, reduced neutrophil infiltration, improved mitochondrial function and suppressed NETosis, compared with those of wild-type (WT) mice. In addition, treatment with FPR1 inhibitor HCH6–1 improved cardiac outcome and inhibits NETosis in CLP mice. Conclusion These data reveal the role of fMet-mediated FPR1/HIF-1α activation in promoting SIC through the NETosis, indicating novel therapeutic strategy for SIC.
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高影响因子
Materials Research Express
·
2024
·Vol. 11
·期 3
·036505
·
IF 1.8
Zhen Jiang,Liduo Chen,Yanhong Cui,Yanhua Suo,Wei Zhang,Yisi Zhou,Yingjun Wang
The monometallic Pt/SAPO-11 and bimetallic Ni-Pt/SAPO-11 catalysts were synthesized with Instant Exactness Synthesis (IES) method and characterized by XRD, nitrogen adsorption–desorption, SEM, TEM, FT-IR, Py-IR, NH3-TPD and XPS, and their properties were evaluated for the n-heptane hydroisomerization. The catalysts had spherical structure and moderate acid strength, and exhibited good n-heptane conversion and catalytic selectivity toward n-heptane isomers. Typically, Ni-Pt/SAPO-11 exhibited an isomer selectivity of 80.9% with a conversion of 69.9% at 310 °C, which was obviously better than Pt/SAPO-11 (isomer selectivity of 75.1% with a conversion of 60.6% at 310 °C). Moreover, the Ni-Pt/SAPO-11 catalyst displayed higher percentage of i-heptane (79%) and lower percentage of n-heptane (6%) than Pt/SAPO-11. The continuous test of 360 min indicated Ni-Pt/SAPO-11 catalyst had good catalytic stability. Bimetallic Ni-Pt alloy loaded on SAPO-11 had significantly effect on catalytic properties of n-heptane hydroisomerization.
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JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
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2024
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IF 2.3
Zhu Mengxuan,Xu Wenqi,Wang Longyang,Wang Rui,Jiang Heng,Jin Changzi
Developing sustainable synthesis method of versatile zeolites to overcome the shortcoming of traditional process is of significant for development of green chemistry and environmentally friendly techniques. In this work, MFI zeolite (ZSM-5) was synthesized through organotemplate-, solvent- and seed-free sustainable process comprising physical grinding and quasi-solid state crystallization and utilizing commercial silica gel or Stöber colloidal SiO 2 as silica source. The key influencing factors to this sustainable synthesis process, such as starting material composition, crystallization temperature and time, had been unambiguously investigated by combining a series of characterization techniques. It is revealed that the starting material with SiO 2 /Al 2 O 3 and Na 2 O/SiO 2 at 30–40 and 0.072, respectively, is suitable to obtain zeolite product with high crystallinity. The presence of right amount of water (or alcohol) is also crucial. In addition, this green synthesis method can be extended into the fabrication of encapsulated metal-zeolite bifunctional catalyst, which is effective in hydroisomerization of n -heptane. These results are instructive for development of sustainable synthesis of aluminosilicate zeolites and derived heterogeneous catalysts. Graphical Abstract
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高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Liuxin Yang,Pengfei Ren,Dandan Wei,Min Liang,Li Xu,Yinghua Tao,Guanhua Jiao,Tianzhu Zhang,Qianli Zhang
Temperature-responsive poly(N-isopropylacrylamide) (PNIPAM) polymers have been studied in many fields due to their “on–off” adjustable properties under temperature stimulation. In this work, the “on–off” mechanism for poly(N-isopropylacrylamide-co-dopamine methacrylamide) (PNIPAM-co-DMA) microgels for cell culture and nonenzymatic cell harvest was explored. Thermoresponsive surfaces were developed on silica wafers using microgels with different DMA contents using a simple spin-coating method. Experiments showed that the microgels showed an “on” state above the lower critical solution temperature (LCST), and PNIPAM-co-DMA (PND) microgels shrank to expose catechol groups for enhanced cell adhesion and proliferation. Conversely, the microgels showed an “off” state below LCST, and the hydrophobic and cell-repellent properties of DMA contributed to the rapid hydration of microgels for accelerated cell disassociation. The developed microgels presented DMA content and distribution-related cell detachment efficiency, with PND (100:0.5) and PND (100:2) showing better results. The failure of cell harvest caused by too much DMA restricted the shrinkage and swelling of microgels due to the strong hydrogen-bonding adhesion of catechol moieties for PND (100:10). Overall, the presented strategy exhibits a wide-ranging utility to construct thermoresponsive surfaces with “on–off” functionalization by introducing catechol chemical monomers.
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Energies
·
2024
·Vol. 17
·期 23
·5811
·
IF 3
Qing Zou,Zhenhui Chen,Siwen Ding,Jimin Xu,Maogang He,Xiangyang Liu
As a renewable energy source and potential substitute for fossil fuels, biodiesel plays an increasingly important role in both energy security and environmental protection. Accurate thermal conductivity data of biodiesels and their mixture with diesel are critical to engine design to achieve high combustion efficiency. This study measured the thermal conductivity of binary mixtures of heptane and biodiesel components, specifically methyl myristate, methyl laurate, and methyl caprate, over a temperature range of 298.15–328.15 K, using the two-wire 3ω method. Based on the experimental data, the effect of mass fraction, temperature, and carbon chain length of the fatty acid ester on the thermal conductivity was analyzed. The second-order Scheffé polynomial model, Flippov equation, Jamieson equation, and Chen equation were used to correlate the experimental data and compare to find a better one. The Flippov equation shows the lowest absolute average relative deviation of 0.80% for the binary mixtures of heptane with methyl myristate, methyl laurate, and methyl caprate.
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高影响因子
JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 89
·期 10
·6707-6719
·
IF 3.2
Qilei Wang,Zhenzhen Feng,Chan Shi,Xingang Lyu,DaiDi Fan
Sulforaphane-loaded nanoparticles (NP-SF) were prepared in this study to improve their biological effects. Based on propylene glycol alginate and zein as wall materials and anthocyanin and CaCl 2 as crosslinking agents, the NPs were encapsulated by the crosslinking method and freeze-dried. With the increasing contents of anthocyanin and Ca 2+ , the encapsulation efficiency and loading capacity of NP-SF were both increased. In vitro simulated digestion experiments showed controlled release of SF from the NPs. The pharmacokinetics confirmed that NP-SF exerted a slower release effect in rats, with improved SF bioavailability and protective effects on liver injury induced by N -diethylnitrosamine in mice. NP-SF reduced serum indicators of liver injury, increased the activities of antioxidant enzymes and GSH levels, and reduced malondialdehyde levels in the liver. In addition, SF activated the Keap1/Nrf2 signaling pathway and upregulated the expression of the Nrf2 downstream genes NQO1 and heme oxidase 1. High doses of NP-SF, in particular, had a higher therapeutic effect. In conclusion, encapsulation enhanced the biological activity of SF and promoted physiological function.
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高影响因子
SOLID STATE SCIENCES
·
2024
·
IF 3.4
Zhan Tian,He Wang,Guoqiang Li,Hongrui Jia,Guoxin Zhang
NiFe 2 O 4 has emerged as an efficient oxygen evolution reaction (OER) electrocatalyst, with outstanding stability in alkaline media and excellent redox properties. In order to further improve the catalytic performance, thinly-walled NiFe 2 O 4 nanotubes (NiFe 2 O 4 -NTs), efficiently derivable from Ni, Fe, N-codoped carbon nanofibers, were innovatively synthesized through a sequential route combing hydrothermal, electrospinning, and high-temperature sintering in this work. The NiFe 2 O 4 -NTs possess large diameter of around 120 nm and their thickness of the tube wall is only about 10 nm. The surface properties of NiFe 2 O 4 can be adjusted by forming the Ni-N/Fe-N bonds. Excitingly, largely exposed active surface area and boosted catalytic reaction kinetics toward oxygen evolution reaction are realized. The required overpotential to deliver 10 mA cm −2 is only 331 mV, accompanied with favorable Tafel slope of only 51.8 mV dec −1 , small charge transfer resistance, and superior reaction stability.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2025
·
IF 21.1
Mengyang Li,Feng Cao,Jie Gao,Guozheng Lv,Na Du,Jiayi Rong,Chongyuan Ruan,Gaowu Qin
Selective reduction of aromatic nitro compounds to functionalized anilines remains a formidable challenge due to the competitive reducible functional groups. High-entropy alloys (HEAs) with multiple elements in near-equiatomic proportions hold great promise given their exceptional physicochemical properties. Herein, we developed nitrogen-doped carbon nanofiber-encapsulated HEA nanoparticles (FeCoNiCrCu@N-CNFs) for efficient nitrobenzene hydrogenation to aniline. The FeCoNiCrCu@N-CNFs catalyst demonstrated exceptional activity with 99 % aniline yield at 60℃, outperforming the FeCoNi@N-CNFs counterpart by a factor of 6.6. The HEA-NC heterojunction was constructed on the surface of the catalyst given the intimate contact between the N-doped carbon layer and the alloy particles. Density functional theory (DFT) calculations revealed that the elevated d‐band center of the HEA, induced by carbon interaction, enhances the intermediates adsorption. This synergistic interplay between HEA and nitrogen-doped carbon provides a novel strategy for designing selective hydrogenation catalysts.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2025
·
IF 2.8
Jundong Yang,Dayu Liang,Xiang Li,Yajun Gao,Wenzhi Wang,Zhimin Lu,Jianlei Qi
Epoxy resin is a widely used thermosetting resin, but its application is limited due to its high brittleness and poor toughness after curing. In this study, we used repolymerized epoxy resin as the matrix and added carboxyl-terminated nitrile rubber (CTBN) and nano-cerium dioxide (n-CeO 2 ) to improve its impact strength without compromising its thermal properties. The test results show that with 15 wt.% CTBN, the tensile strength of the cured product is 60.59 MPa, the flexural strength is 132 MPa, and the impact strength is 22 KJ/m 2 , which are 206%, 200%, and 424% higher than those of pure epoxy resin, respectively. To further enhance the toughening effect, we introduced n-CeO 2 on the basis of 15 wt.% CTBN. The study found that with increasing n-CeO 2 content, the impact strength first increased and then decreased. When the n-CeO 2 content is 2 wt.%, the impact strength of the resin reaches 29.3 KJ/m 2 , which is 32% higher than that of CTBN. Additionally, thermogravimetric analysis (TGA) shows that the addition of n-CeO 2 significantly improves the thermal stability of CTBN/EP composites. Scanning electron microscopy (SEM) analysis reveals that CTBN cavitation occurs in the resin; n-CeO 2 plays a crucial role in particle debonding and crack pinning.
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Science Bulletin
·
2024
·
IF 18.8
Xia Jie,Zhang Lixing,Zhu Wucheng,Tu Juchuanli,Peng Xilei,Deng Qiaodan,Li Siqin,He Xueyan,Dong Haonan,Liu Cuicui,Chen Xian,Xu Jiahui,Ma Wei,Xiao Yi,Liu Wen,Hu Guohong,Jiang Yi-Zhou,Chen Ceshi,Bian Xiu-Wu,Shao Zhi-Ming,Liu Suling
Neurotransmitters are increasingly recognized to play important roles in limiting anti-tumor immunity. N-acetyl-aspartyl-glutamate (NAAG) has been extensively studied in neurological disorders; however, its potential role in restricting anti-tumor immunity has not been investigated. Here, we demonstrated that NAAG or its synthetase RimK-like family member B (RIMKLB) significantly disrupted anti-tumor immunity by rewiring the myeloid progenitor differentiation of polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs), which in turn promoted breast cancer growth and metastasis. Mechanistically, NAAG sustained the tumor immunosuppressive microenvironment by activating an NR2B-containing NMDA receptor (NR2B-NMDAR)-dependent p38-NOTCH1 axis, and subsequently stimulating tumor cell migration and invasion, as well as inducing PMN-MDSC differentiation and expansion. In mouse models, RIMKLB ablation or NMDAR inhibition enhanced the efficacy of anti-PD-1 therapy and suppressed tumor progression. An analysis of clinical samples revealed that high levels of NAAG and NR2B-NMDAR predicted a poor prognosis in TNBC patients. Collectively, our findings have uncovered a signaling role for tumor-derived NAAG beyond its classic function as a neurotransmitter that can be targeted pharmacologically to enhance immunotherapy against breast cancer.
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