APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2017
·Vol. 101
·期 14
·5689-5697
·
IF 3.42
Zhou Pengfei,Lan Dongming,Popowicz Grzegorz Maria,Wang Xuping,Yang Bo,Wang Yonghua
Green technologies are attracting increasing attention in industrial chemistry where enzymatic reactions can replace dangerous and environmentally unfriendly chemical processes. In situ enzymatic synthesis of peroxycarboxylic acid is an attractive alternative for several industrial applications although concentrated H 2 O 2 can denature the biocatalyst, limiting its usefulness. Herein, we report the structure-guided engineering of the Pyrobaculum calidifontis esterase (PestE) substrate binding site to increase its stability and perhydrolysis activity. The L89R/L40A PestE mutant showed better tolerance toward concentrated H 2 O 2 compared with wild-type PestE , and retained over 72% of its initial activity after 24-h incubation with 2 M H 2 O 2 . Surprisingly, the half-life ( t
1/2 , 80 °C) of PestE increased from 28 to 54 h. The k
cat / K
m values of the mutant increased 21- and 3.4-fold toward pentanoic acid and H 2 O 2, respectively. This work shows how protein engineering can be used to enhance the H 2 O 2 resistance and catalytic efficiency of an enzyme.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-8
·
IF 4.259
Yang Yufang,Shen Dongjun,Long Yijuan,Xie Zhixiong,Zheng Huzhi
Ficin is classified as a sulfhydryl protease isolated from the latex of fig trees. In most cases, a particular enzyme fits a few types of substrate and catalyzes one type of reaction. In this investigation, we found sufficient proofs for the intrinsic peroxidase-like activity of ficin and designed experiments to examine its effectiveness in a variety of scenarios. Ficin can transform peroxidase substrates to colored products in the existence of H 2 O 2 . Our results also indicate that the active sites of peroxidase-like activity of ficin are different from that of protease, which reveals that one enzyme may catalyze more than one kind of substrate to perform different types of reactions. On the basis of these findings, H 2 O 2 releasing from MCF-7 cells was detected successfully. Our findings support a wider application of ficin in biochemistry and open up the possibility of utilizing ficin as enzymatic mimics in biotechnology and environmental monitoring.
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POWDER TECHNOLOGY
·
2017
·
IF 2.942
Liang Shen,Jinbo Zhu,Lingyun Liu,Huaifa Wang
In order to improve the flotation recovery of fine kaolinite , mixed dodecylamine chloride/fatty acid was used as collector in kaolinite flotation process. The performance of dodecylamine chloride/fatty acid mixture collector on kaolinite flotation was investigated using flotation test and computational method. Surface tension measurement, contact angle measurement and image analysis were also used to study the flotation behavior of dodecylamine chloride and mixture collectors. The flotation results showed that much higher kaolinite flotation recovery was obtained in the presence of dodecylamine chloride/octanoic acid mixture compared with that in the presence of dodecylamine chloride alone. Surface tension measurements indicated that the mixed dodecylamine chloride/octanoic acid collector was more efficient at decreasing the air–water interfacial tension. Molecular dynamics simulation showed that the octanoic acid molecules were interleaved among dodecylamine chloride ions and co-adsorbed at the kaolinite (001) surface. The relative concentration of water molecules near kaolinite (001) surface further decreased in the presence of dodecylamine chloride/octanoic acid compared with that in the presence of dodecylamine chloride alone, which indicated a much stronger hydrophobicity of the kaolinite (001) surface.
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FISH & SHELLFISH IMMUNOLOGY
·
2024
·
IF 4.1
Nanhai Xiao,Xiuzhen Zhou,Yi Zhang,Tao Liu,Yiwei Jiang,Sami Ullah,Jing Wang,Fengqin Feng,Minjie Zhao
The aim of this study was to investigate the effects of antibacterial peptides from Brevibacillus texasporus (BT) on the growth performance, meat quality and gut health of cultured largemouth bass ( Micropterus salmoides ). Largemouth bass (36.17 ± 1.52 g) were divided into 2 groups and each group was fed with diets supplemented with or without 200 ppm of BT peptides for 130 days. The results showed that BT peptides had no significant influences on growth performance and body indexes, but significantly enhanced total antioxidant capacity and lysozyme content in the serum. Moreover, digestive enzymes activities and intestinal villous height were also prominently increased. From meat quality aspect, no significant differences were found in nutritional components, amino acid composition, fatty acid composition and texture property, except the values of hardness, gumminess and γ-linolenic acid (C18:3n6) were remarkably increased after BT peptides intervention. Finally, the results of gut microbiota and short chain fatty acids revealed that BT peptides significantly decreased the relative abundances of harmful bacteria such as genus Acinetobacter and Pseudomonas , and increased the production of short chain fatty acids. In conclusion, this study confirmed that BT peptides could be used to improve the health of largemouth bass, which provided novel insights into the application of antimicrobial peptides in aquacultures.
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Journal of Environmental Sciences
·
2024
·
IF 5.9
Xiao Liu,Xiaolong Zhang,Chunyu Geng,Qinglan Hao,Jie Chang,Xin Hu,Yongwang Li,Botao Teng
Fischer-Tropsch synthesis (FTS) wastewater retaining low-carbon alcohols and acids are organic pollutants as a limiting factor for FTS industrialization. In this work, the structure-capacity relationships between alcohol-acid adsorption and surface species on graphene were reported, shedding light into their intricate interactions. The graphene oxide (GO) and reduced graphene oxide (rGO) were synthesized via improved Hummers method with flake graphite (G). The physicochemical properties of samples were characterized via SEM, XRD, XPS, FT-IR, and Raman. The alcohol-acid adsorption behaviors and adsorption quantities on G, GO, and rGO were measured via theoretical and experimental method. It was revealed that the presence of COOH, C=O and C O species on graphene occupy the adsorption sites and increase the interactions of water with graphene, which are unfavorable for alcohol-acid adsorption. The equilibrium adsorption quantities of alcohols and acids grow in pace with carbon number. The monolayer adsorption occurs on graphene was verified via model fitting. rGO has the highest FTS modeling wastewater adsorption quantity (110 mg/g) due to the reduction of oxygen species. These novel findings provide a foundation for the alcohol-acid wastewater treatment, as well as the design and development of high-performance carbon-based adsorbent materials.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Haocheng Li,Yuanyuan Li,Lin Liu,Xiangbo Ren,Chunlong Yuan,Junjun Li
This study evaluated the impact of Artemisia sphaerocephala Krasch glucomannan with different degrees of acetyl group substitution (DS) on the prebiotic properties of grape seed proanthocyanidins (GSP). UV spectra, CIELab, and dynamic light scattering analyses indicated DS-influenced variable interactions between GSP and glucomannan. In vitro fermentation demonstrated that glucomannan enhanced the solubility of some phenolic compounds, and decreased the pH value of fermentation liquids. The production of acetate acid and total short chain fatty acids in the GSP fermentation liquid increased with the degree of DS of glucomannan. Notably, acetylated glucomannan exerted dramatic effects on GSP-induced gut microbiota modulation. The relative abundances of Bacteroides ovatus and Bacteroides decreased as DS increased. Meanwhile, Bacteroides acidifaciens and Akkermansia muciniphila have a positive correlation, even though the GSP-promoted enrichment of A. muciniphila was inhibited by the added glucomannan. Moreover, glucomannan enhanced the metabolism of nucleotides, secondary bile acid, and glycan inhibited by GSP. These findings suggest that acetylated glucomannan affect the prebiotic properties of GSP with its DS serving as a key factor.
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FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Fang Wu,Yaqiong Liu,Ming Zhang,Xinlei Yuan,Tengteng Ji,Yutong Jin,Yixuan Li,Ran Wang,Yanling Hao,Bing Fang
Recent studies have shown that 1-oleo-2-palmito-3-linoleyl glycerol (OPL) is the most abundant triacylglycerol in human breast milk in China. Epidemiologic studies have shown that sn -2 palmitate improves the absorption of fatty acids and calcium in infants. However, there have been few studies of the specific mechanism by which OPL affects intestinal function. In the present study, we have characterized the effects of various levels of OPL supplementation on the development of the intestinal epithelium and the intestinal microbiota of neonatal mice. OPL supplementation increased the body masses and intestinal lengths of weaned mice and promoted defecation. These positive effects were related to the effect of OPL to promote the development of intestinal villi and crypts. OPL increased the expression of the intestinal stem cell markers Olfm4 and Sox9 in the jejunum and ileum, which promoted their differentiation into goblet cells and Paneth cells. It also promoted the integrity of the epithelial barrier by increasing the secretion of mucin 2 and lysozyme 1 and the expression of the tight junction proteins occludin, ZO1, claudin 2, and claudin 3. More importantly, we found that low dose-OPL promotes the transformation of the intestinal microbiota of neonatal mice to the mature state in 3-month-old mice, increases the proportion of Firmicutes, and reduces the proportion of Bacteroidota. The proportions of anaerobic genera of bacteria, such as Lachnospiraceae_NK4A136_group, Lachnoclostridium, Ligilactobacillus, and Bifidobacterium were higher, as were the key producers of short-chain fatty acids, such as Bacteroides and Blautia . OPL also increased the butyric acid content of the feces, which significantly correlated with the abundance of Lactobacillus . High-dose OPL tended to be more effective at promoting defecation and the development of the villi and crypts, but these effects did not significantly differ from those achieved using the lower dose. A low dose of OPL was more effective at increasing the butyric acid content and causing the maturation of microbes. In summary, the OPL supplementation of newborn mice promotes the establishment of the intestinal epithelial layer structure and barrier function, and also promotes the transformation of the intestinal microbiota to a mature state. This study lays a theoretical foundation for the inclusion of OPL in infant formula and provides a scientific basis for the development of intestinal health products.
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MOLECULAR NUTRITION & FOOD RESEARCH
·
2025
·
IF 4.2
Fang Wu,Yaqiong Liu,Ming Zhang,Xinlei Yuan,Yutong Jin,Yixuan Li,Ran Wang,Yanling Hao,Bing Fang
1,3-Dioleoyl-2-palmitoyl-glycerol (OPO) is a specific triglyceride in human breast milk, and it has been added to infant formula to mimic human breast milk fat. Existing studies only focused on its effects on fatty acid and calcium absorption, as well as the intestinal microbial composition; however, effects of OPO on the early-life development of intestine were still unclear. Our study explored the effects of OPO on intestinal epithelial structure and barrier construction in neonatal mice and the involvement of intestinal microorganisms. OPO supplementation significantly increased the number of intestinal stem cells, which in turn promoted villus and crypt, and promoted goblet cell and Paneth cell differentiation. OPO also promotes epithelial barrier integrity by increasing the expression of mucin 2, lysozyme 1, and tight junction proteins. Furthermore, the benefits of OPO were associated with the higher abundance of beneficial bacteria ( unclassified_f_Muribaculaceae , Akkermansia , Bifidobacterium , and Blautia ) and elevated butyrate levels. This study demonstrates the efficacy of OPO on intestinal health in neonatal mice beyond defecation, expands the understanding of the biological functions of OPO, and expands its application in intestinal health products targeting special populations, such as the elderly or individuals with intestinal fragility or injury.
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Frontiers in Microbiology
·
2025
·
IF 4.5
Mengge Sun,Haotian Huang,Haibao Tang,Jiajie Chen,Wei Chen,Dongsheng Yang
Recent studies have highlighted the prebiotic potential of natural plant polysaccharides, demonstrating their role in promoting beneficial gut microbiota and improving health. However, research on the digestive properties and prebiotic activities of Imperatae Rhizoma Polysaccharides (IRPs) remains limited. This study investigated fresh Imperatae Rhizoma as the research object. After processing, dry Imperatae Rhizoma and carbonized Imperatae Rhizoma were prepared. Three polysaccharides from the fresh, dry, and carbonized Imperatae Rhizoma were extracted with traditional hot water. And another polysaccharide was obtained by cold water extraction from fresh Imperatae Rhizoma. Total four IRPs were extracted and named: IRPs-F, IRPs-D, IRPs-C, and IRPs-J. This study evaluated the prebiotic activity of four polysaccharides derived from the roots of thatch, demonstrating their resistance to digestion, their ability to promote probiotic growth, and their enhancement of short-chain fatty acid (SCFA) production. The final results show that four IRPs exhibit strong resistance to digestion and IRPs-F ability to promote the growth of beneficial probiotics, making it a promising candidate for functional foods aimed at improving intestinal health, immune regulation, and metabolic benefits. This research is highly relevant to food microbiology and holds significant potential for application in the functional food and gut health sectors.
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Food Bioscience
·
2024
·
IF 4.8
Xiaona Sun,Yunning Yang,Chen Song,Chunqing Ai,Jingfeng Yang,Shuang Song
Fucoidan, a sulfated polysaccharide from brown algae, has been shown to improve gut microbiota balance and function. However, the relationship between molecular weight of fucoidan and colonic fermentation characteristics is still unclear. Here, the interaction between different-molecular-weight fucoidans and intestinal microbiota was analyzed via an in vitro fecal fermentation model. Results showed that fucoidan and its 2 low-molecular-weight derivatives (Dfuc1 and Dfuc2) could be utilized by intestinal microbiota after 48 h of fermentation, and among them, Dfuc2 exhibited the highest utilization efficiency of 30.1%, followed by Dfuc1 (24.5%) and fucoidan (14.4%). Notably, the components glucose, mannose and galactose, but not fucose, in different-molecular-weight fucoidans could be utilized. Moreover, intestinal microbiota could degrade Dfuc1 and Dfuc2 to produce oligosaccharide fragments, such as fucose-hexose disaccharide. In addition, fucoidan, Dfuc1 and Dfuc2 displayed different prebiotic effects on the intestinal microbiota community. Native fucoidan preferred to promote Bacteroides and Lactococcus , and low-molecular-weight fucoidans tended to promote Parabacteroides and Lactobacillus . Furthermore, metabolites analysis showed that fucoidan, Dfuc1 and Dfuc2 had similar promoting effects on acetic acid and propionic acid, and had no effect on butyric acid. But they could regulate carbohydrate metabolic pathways and amino acid pathways to varying degrees and with decreasing molecular weight, more differential metabolites were found. Thus, Dfuc2 with a lower molecular weight has more potential to become a potential prebiotics to improve intestinal health.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2025
·
IF 3.1
Qiuyi Jing,Hong Pan,Xiaoli Li,Yaya Fan,Jingshan Shi,Chao Fang,Fuguo Shi
Catalpol, an iridoid glycoside, exhibits potent and versatile pharmacological effects. It is a promising drug candidate for treating ischemic stroke. However, its drug metabolism and disposition remain poorly understood, which hinders a better understanding of its mode of action. Here, we elucidated the intriguing metabolic characteristics of catalpol in rats. Catalpol underwent sequential metabolism mainly in the intestine, resulting in 31 stable metabolites and 8 unstable dialdehyde metabolites. Twelve glucosyl-containing metabolites were generated through the direct metabolism of catalpol. Eleven deglycosylated metabolites were primarily derived from catalpol aglycone metabolism. Seven N -heterocyclic metabolites originated from aglycone metabolites. Eight unstable and reactive dialdehyde metabolites were formed by the ring-opening of the hemiacetal reaction in aglycone metabolites. Eleven metabolic pathways were involved in catalpol metabolism. All eight dialdehyde metabolites were produced in the intestine through the sequential metabolism of catalpol. Notably, five dialdehyde metabolites could covalently bind to the proteins in the intestine. The dialdehyde metabolites were primarily derived from didehydroxylated and acetylated aglycone. Catalpol could improve the levels of gut bacterial metabolites, short-chain fatty acids. In conclusion, catalpol underwent extensive and sequential metabolism, generating 31 stable metabolites and 8 reactive dialdehyde metabolites. Five dialdehyde metabolites enable covalent protein modification in the intestine, which may be vital to the potent and versatile pharmacological effects of catalpol.
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Foods
·
2025
·Vol. 14
·期 1
·101
·
IF 5.1
Run Yu,Haimeng Zhang,Rui Chen,Yangzhuo Lin,Jingxuan Xu,Ziyang Fang,Yuehang Ru,Chenhan Fan,Guoqing Wu
Alzheimer’s disease (AD) is marked by impaired cognitive functions, particularly in learning and memory, owing to complex and diverse mechanisms. Methionine restriction (MR) has been found to exert a mitigating effect on brain oxidative stress to improve AD. However, the bidirectional crosstalk between the gut and brain through which MR enhances learning and memory in AD, as well as the effects of fecal microbiota transplantation (FMT) from MR mice on AD mice, remains underexplored. In this study, APP/PS1 double transgenic AD mice were used and an FMT experiment was conducted. 16S rRNA gene sequencing, targeted metabolomics, and microbial metabolite short-chain fatty acids (SCFAs) of feces samples were analyzed. The results showed that MR reversed the reduction in SCFAs induced by AD, and further activated the free fatty acid receptors, FFAR2 and FFAR3, as well as the transport protein MCT1, thereby signaling to the brain to mitigate inflammation and enhance the learning and memory capabilities. Furthermore, the FMT experiment from methionine-restricted diet mouse donors showed that mice receiving FMT ameliorated Alzheimer’s learning and memory ability through SCFAs. This study offers novel non-pharmaceutical intervention strategies for AD prevention.
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