Frontiers in Microbiology
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2021
·
IF 5.64
Chen Guo,Shuhan You,Changmei Li,Tiantian Chen,Xiudan Wang
The global food waste problem, especially aquatic product spoilage, stimulates the accurate freshness analysis of food products. However, it still remains a great challenge to realize in-field determination of fish freshness at the time of use. In the present study, a colorimetric enzyme biosensor was developed for one-step detection of hypoxanthine (Hx), which is an important intermediate of adenosine triphosphate decomposition during fish storage. We demonstrate that xanthine oxidase grade I ammonium sulfate suspension (XOD-ASS) possesses peroxidase activity. It can oxidize different peroxidase substrates, including 3,3′,5,5′-tetramethylbenzidine, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, and o-phenylenediamine in the presence of H 2 O 2 , producing visible color reactions. Further experiments indicate that XOD-ASS displayed effective peroxidase activity and could be used for H 2 O 2 detection. Based on this, a one-step Hx detection method was established using only XOD-ASS as the catalyst. The method displays a good linear relationship in the range from 20 to 100 μM with a detection limit of 6.93 μM. Additionally, we successfully applied this method in testing Hx accumulation in sea bass fish samples of different storage times. The recovery values range from 97.44 to 102.56%. It is exciting to note that, compared with other methods, our proposed method provides a robust advantage on the economic reaction system, ease of preparation, short time consumption, and moderate reaction temperature. We believe that this method shows good application prospects for on-site fish freshness determination.
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BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Tianyu Li,Bo Liang,Zhichao Ye,Lei Zhang,Shiyi Xu,Tingting Tu,Yiming Zhang,Yu Cai,Bin Zhang,Lu Fang,Xiyu Mao,Shanshan Zhang,Guan Wu,Qifu Yang,Congcong Zhou,Xiujun Cai,Xuesong Ye
Simultaneous monitoring of electrophysiological and biochemical signals is of great importance in healthcare and fitness management, while the fabrication of highly integrated and flexible devices is crucial to these applications. Herein, we devised a multifunctional and flexible hydrogel-paper patch (HPP) that was capable of simultaneously real-time monitoring of electrocardiogram (ECG) signal and biochemical signal (glucose content) in sweat during exercise. The self-assembly of the highly porous PEDOT:PSS hydrogel on paper fiber provided the HPP with good conductivity and hydrophilic wettability for efficient electron transmission and substance diffusion, thereby enabling it to serve as a low-impedance ECG electrode and a highly sensitive glucose sensor . Additionally, the spontaneous capillary flow effect allows the paper patch to be used as microfluidic channels for the collect and analysis of sweat. Moreover, the HPP is integrated with a flexible printed circuit board (FPCB) and works as a multifunctional wearable device mounted on the chest for real-time monitoring of electrophysiological and biochemical signals during exercise.
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TALANTA
·
2021
·
IF 6.057
Yang Tao,Yao Wang,Rufeng Zhu,Yuanli Chen,Xue Liu,Mufang Li,Liyan Yang,Yuedan Wang,Dong Wang
In this study, poly(3, 4-ethylenedioxythiophene) (PEDOT) nanocluster structure was synthesized on the reduced graphene oxide (rGO) modified cotton fibers. The organic electrochemical transistors based on the modified fiber have been assembled and their performance of different gate electrode transistors has been investigated. The transistor exhibits an excellent transconductance of up to 15.5 mS and a high on-off ratio close to 2*10 2 . The bending angle and bending times have little effect on the device performance. The uric acid (UA) sensor based transistor has been fabricated for the first time. Flexible sensors based on molecularly imprinted polymer (MIP) membrane with different fiber gate electrodes have been investigated. The UA sensor with MIP/PEDOT/carbon fiber as the gate electrode has a sensitivity of 100 μA per decade from 1 nM to 500 μM, a linear coefficient of 0.97143, excellent selectivity, and good reproducibility. In addition, fiber based organic electrochemical transistors (FECTs) can be sewn into the fabric for monitoring and have successfully evaluated the detection of UA in artificial urine sample, with data consistent well with the UA concentration obtained from single fiber. Therefore, the sensor based FECTs can be used for low cost, accurate, non-enzymatic detection of UA in clinical diagnostics and bioanalytical applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Ya You,Jiao Zou,Wen-Jie Li,Jun Chen,Xin-Yu Jiang,Jin-Gang Yu
The abnormal levels of two biological molecules, dopamine (DA) and Uric acid (UA), in human body are symptoms of diseases such as Parkinson's disease and arrhythmia. A novel lanthanum vanadate and multi-walled carbon nanotubes (LaV-MWCNTs) composite modified glassy carbon electrode (GCE) was developed and utilized as an efficient electrochemical sensor for the simultaneous detection of DA and UA. LaV-MWCNTs composite was successfully fabricated by a facile ultrasonic self-assembly method and identified by means of a series of successive measurements including XPS, XRD, FT-IR and FE-SEM. The LaV-MWCNTs modified GCE shows the concentration linear ranges of DA and UA are 2–100 μΜ using DPV. The limits of detection (LODs; signal-to-noise ratio of 3, S/N = 3) of the LaV-MWCNTs modified GCE sensor for DA and UA were calculated to be 0.046 μM and 0.025 μM, respectively. The feasibility of using the LaV-MWCNTs modified GCE sensor to detect DA and UA in a typical biological fluid, fetal bovine serum, was also evaluated by the standard addition method.
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MICROCHIMICA ACTA
·
2021
·Vol. 189
·期 1
·1-11
·
IF 5.833
Ma Junping,Bai Wushuang,Liu Xiaoli,Zheng Jianbin
Integrating other metal ions into mono-metallic metal–organic framework (MOF) to form bi-metallic MOF is an effective strategy to enhance the performance of MOFs from the internal structure. In this study, two-dimensional (2D) cobalt/zinc-porphyrin (Co/Zn-TCPP) MOF nanomaterials with different Co/Zn molar ratios were synthesised using a simple surfactant-assisted method, and novel dopamine (DA) sensing methods were constructed based on these materials. The characterisation results showed that all MOF with different Co/Zn molar ratios presented a nanofilm, and the Co and Zn elements were uniformly distributed. All sensors based on Co x Zn 100− x -TCPP had a certain catalytic performance to DA. Among them, the sensor based on CO 25 Zn 75 -TCPP showed the strongest signal response, indicating that the catalytic performance of MOF on DA can be adjusted by changing the Co/Zn molar ratio. The doping of metal ions improves the chemical environment of the pores, and increases the types and spatial arrangement of the active sites of the MOF, which is beneficial to the electron transfer and exchange with DA; Co 2+ and Zn 2+ active centres have a synergistic promotion effect, so the catalytic activity of MOF is significantly improved. The linear range at the potential of 0.1 V based on Co 25 Zn 75 -TCPP for DA was 5 nM–177.8 μM, with a detection limit of 1.67 nM (S/N = 3). The sensor exhibited a good selectivity for detecting DA. This research is expected to provide new ideas and references for constructing high-performance sensing interfaces and platforms. Graphical abstract
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Nanoscale
·
2021
·Vol. 13
·期 47
·20078-20090
·
IF 7.79
Kai Zhang,Zhonghui Zhuo,Guorong Fan,Zongde Wang,Shangxing Chen,Lulu Xu,Yangping Wen,Peng Wang
The simultaneous detection of multiple biological small molecules is critical for human health evaluation and disease prevention. In this study, a nano-ZnS decorated hierarchically porous carbon (ZSHPC) electrocatalyst with multiple enzyme-like activities as a nanozyme sensing platform for simultaneous derivative voltametric detection of four important biological small molecules, dopamine (DA), uric acid (UA), guanine (G), and adenine (A), is successfully synthesized via an in situ hydrothermal reaction using leaves of Cinnamomum camphora (L.) after the extraction of essential oil as a carbon source, ZnCl2 as both zinc source and an activator, sulfuric acid as a sulfur source, and silica gel as a hard template. Activator together with the introduction of silica gel is beneficial for tuning pore structure. The in situ synthesized ZnS nanoparticles and sulfur doping improve the conductivity and cycling stability of the material. The ZSHPC electrode with multiple enzyme-like activities and oxidase-like characteristics was employed for the simultaneous detection of multiple target molecules in linear ranges of 0.3–500 μM with detection limits of 0.12 μM for DA, 0.26 μM for UA, 0.07 μM for G, and 0.075 μM for A. A derivative technique was selected for enhancing the peak resolution of the partial overlapped voltammograms and eliminating human error. Both the coefficient of determination and residual prediction deviation were used to evaluate this technique.
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Jing Chen,Shuying Li,Ying Chen,Jiao Yang,Jianbin Dong,Xiaoquan Lu
5-Hydroxytryptamine (5-HT) is an important neurotransmitter, and its content in the human body is of great significance to human health. In this study, an l-cysteine-terminated triangular silver nanoplate loaded on a MXene (two-dimensional transition metal carbide or nitride) (Tri-AgNP/l-Cys/MXene) electrochemical sensor was used to detect 5-HT. As an electrically active amino acid with a sulfhydryl group, l-cysteine (l-Cys) forms a more stable Ag–S bond with silver nanoparticles, which can selectively substitute trisodium citrate (TSC) in TSC-capped triangular silver nanoplates (Tri-Ag-NP/TSC). Due to the good conductivity, biocompatibility, and large surface area, MXenes provide a good platform for loading Tri-AgNP/l-Cys. Under optimized conditions, the concentration range for detecting 5-HT with the sensor is 0.5–150 μM, and the limit of detection (LOD) is 0.08 μM (S/N = 3). For detecting 5-HT in actual serum samples, the sensor also showed a good recovery rate (95.38–102.3%), and the relative standard deviation was 2.2–3.4%.
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LUMINESCENCE
·
2021
·Vol. 37
·期 2
·340-347
·
IF 2.464
Liping Lin,Qingjiao Lai,Qiushuang Chen,Qiaoling Zheng,Meng Fang,Xinghao He,Jinying Qin
Hydrothermal treatment of m -phenylenediamine and grape seed powder has been adopted to synthesize nitrogen-doped carbon quantum dots (N-CQDs). The prepared N-CQDs possessed outstanding optical properties and high quantum yield. Based on the combined effect of static quenching effect and inner filter effect of permanganate (MnO 4 − ) to N-CQDs and the redox reaction that occurred between MnO 4 − and l -ascorbic acid ( l -AA), an ‘off–on’ fluorescence strategy with N-CQDs has been proposed for the detection of MnO 4 − and l -AA. The proposed fluorescent probe was fast, sensitive and selective to MnO 4 − and l -AA under mild conditions. In addition, the satisfactory results of the proposed strategy for the detection of MnO 4 − and l -AA in real samples indicated its practicability.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Zehuai Mou,Zhijin Gao,Yaoping Hu
Sensitive determination of hypoxanthine (HX), an indicator reflecting the degradation of meat and fish, is significantly important in monitoring food freshness. Herein, we design a novel sensor consisting of orange emissive carbon dots (O-CDs), nitrotetrazolium blue chloride (NTBC), and xanthine oxidase (XOD) for fluorescence turn-off detection of HX. O-CDs, possessing a high fluorescence quantum yield of 37%, are synthesized by hydrothermal treatment of 2,3-diaminopyridine in sulfuric acid. NTBC can react with HX/XOD-generated H 2 O 2 and O 2 – to yield a violet-colored formazan, which remarkably quenches the orange fluorescence of O-CDs through inner filter effect. There is a linearity between the quenching efficiency and HX concentration in the range of 2–250 μM, and the limit of detection is 0.61 μM, lower than those of most reported HX sensors. In addition, the proposed method exhibits excellent selectivity, and can be applied to quantify HX in fish samples with satisfactory results.
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BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Yuan Zhao,Jie Yao,Zhenglong Wu,Han Liu,Wangwang Zheng
Strong and stable electrochemical beacons are critical for the achievement of sensitive and reliable electroanalysis applications. In this work, the electrochemical oxidation performance of AuPt NPs was studied and firstly found to be largely enhanced under light illumination. Plasmonic AuPt NPs collected light energy after local surface plasmon resonance (LSPR) excitation and generated much more holes to participate in the electrochemical oxidation process of Pt 0 in AuPt NPs. AuPt NPs with the electrochemical oxidation peak at around −0.7 V were utilized as detection probes for the fabrication of ratiometric electrochemical aptasensor , by introducing Co-MOF/Fe 3 O 4 /Ag nanosheets (NSs) with the electrochemical oxidation peak at 0.1 V as reference beacons. The aptamers of epithelial cell adhesion molecule (EpCAM) modified AuPt NPs were assembled with Co-MOF/Fe 3 O 4 /Ag NSs, which generated strong detection and reference signals at −0.7 V and 0.1 V, respectively. The high affinity between EpCAM and aptamers induced the separation of AuPt NPs from Co-MOF/Fe 3 O 4 /Ag NSs, resulting in the decrease of detection signal at −0.7 V and unchanged reference signal at 0.1 V. A ratiometric electrochemical aptasensor was achieved for the sensitive and reliable quantification of EpCAM in the range from 100 pg/mL to 100 ng/mL. The limit of detection (LOD) was calculated to be 13.8 pg/mL for EpCAM. Plasmon-driven electrochemical oxidation enhancement principle provides the possibility for the design and fabrication of more strong and anti-interference electroactive plasmonic metal-Pt composite nanostructures for the electroanalysis applications.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Shen Zhang,Jinjia Bao,Ling Lv,Yongjie Bai,Yiming Zhang,Yaofang Zhang
The non-standard use of curcumin could cause some adverse drug reactions, such as diarrhea, nausea and skin allergies. Thus, the curcumin determination was fundamental to disease treatment and prevention. Herein, a facile and efficient fluorescent probe was developed based on carbon dots, which was prepared through hydrothermal method (o-phenylenediamine and N-isopropylacrylamide as the reaction raw materials). Characteristics of the as-fabricated carbon dots (NCDs) were studied through some analysis techniques, such as UV–vis absorption spectroscopy, transmission electron microscopy, Fourier transform infrared instrument, X-ray photoelectron spectroscopy and fluorescence spectrophotometer. Fluorescence quenching phenomenon could be observed after addition of curcumin. This as-prepared fluorescent probe displayed a significant response for the determination of curcumin with a satisfactorily lower detection limit of 0.017 μM and a considerable linear range of 0.5–50 μM compared to reported literatures. Because of the preeminent repeatability and anti-jamming capability, the as-developed CDs suggested mighty potentiality for actual applications of curcumin detection in real samples and temperature sensing.
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JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2023
·Vol. 170
·期 12
·127506
·
IF 3.9
Lingling Han,Yali Yang,Bowen Wang,Fuxin Liu,Na Li,Dongyu Zhang,Xiuhui Liu
It is well known that elevated homocysteine (HcySH) values can lead to a wide range of diseases. Quantifying HcySH can be an aid in early diagnosis and treatment of related illnesses. Consequently, it is very significant to develop a sensitive detection technology for exact monitoring of HcySH. Based on the excellent affinity of Au NPs with the -SH group in HcySH and the excellent conductivity of cMWCNTs, a novel electrochemical sensor (Au NPs/cMWCNTs/GCE) was constructed to detect HcySH in this paper. The sensor not only can effectively improve the specificity of HcySH detection, but also has the wide linear range and low detection limit (2.89 × 10−8 M, S/N = 3). What's more, the homocysteine contents of a variety of serum samples were directly detected by the constructed sensor for the first time, and the recovery rate was satisfactory. In conclusion, the research provided an effective method to achieve the rapid, reliable and ultrasensitive determination of HcySH, which has important applications in biomedical analysis and clinical diagnosis.
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