BIOLOGICAL & PHARMACEUTICAL BULLETIN
·
2025
·Vol. 48
·期 5
·555-562
·
IF 1.7
Junli Han,Lianghe Wang,Li Wang,Hua Lei
Pulmonary arterial hypertension (PAH) is a progressive disorder that lacks a validated and effective therapy. Thus, further investigation of the pathogenesis of PAH will help explore novel treatments. The increase in T helper 17 (Th17) cell-mediated pro-inflammatory response and reduction of regulatory T (Treg) cell-mediated anti-inflammatory effect exacerbates PAH progression. Increasing evidence indicates that 5-hydroxytryptamine (5-HT) is closely related to Th17 and Treg polarization. Here, a decrease of 5-HT was found in hypoxia-induced CD4 + T cells. Hypoxia also resulted in a reduction in Treg cells and an increase in Th17 cells, but the addition of 5-HT rescued Th17/Treg balance, confirming that hypoxia destroyed Th17/Treg balance by inducing a 5-HT decrease. Furthermore, we found that 5-HT-restored Th17/Treg balance mitigated primary pulmonary artery smooth muscle cell (PASMC) proliferation, migration, and contraction, which are important factors in vascular remodeling in PAH. In summary, our findings demonstrate that hypoxia-induced 5-HT decline interferes with the balance of Th17/Treg, which affects the biofunction of PASMCs, thus accelerating PAH development. 5-HT-mediated Th17/Treg balance is expected to act as a novel immunotherapy for PAH treatment.
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高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2015
·
IF 3.453
Chaoqun Bian,Qinming Wu,Jian Zhang,Fang Chen,Shuxiang Pan,Liang Wang,Xiangju Meng,Ulrich Müller,Mathias Feyen,Bilge Yilmaz,Hermann Gies,Weiping Zhang,Xinhe Bao,Dirk De Vos,Toshiyuki Yokoi,Takashi Tatsumi,Feng-Shou Xiao
The layered silicate COK-5 has been used for an interlayer expansion reaction with dichlorodimethylsilane (DCDMS) at 180 °C to interconnect neighboring layers, yielding a new and crystalline microporous framework. The samples containing the methyl functional groups in the as-made form and having OH groups in the calcined form were designed as COE-5 and calcined COE-5. These samples were characterized with X-ray diffraction (XRD), N 2 sorption isotherms, inductively coupled plasma optical emission spectrometry (ICP-OES), infrared spectroscopy (IR), high-resolution transmission electron micrograph (HRTEM), thermogravimetry-differential thermal analysis (TG-DTA), and 29 Si and 13 C solid-state magic-angle spinning nuclear magnetic resonance (MAS NMR), as well as the contact angle techniques. XRD patterns and HRTEM images suggest that the sample interlayer spacing has been expanded by nearly 0.5 Å. The N 2 sorption isotherms of the materials show the BET surface areas are 165 m 2 /g for COE-5 and 340 m 2 /g for calcined COE-5. 29 Si and 13 C MAS NMR as well as IR spectroscopy confirm the insertion of the linker group -Si(CH 3 ) 2 - connecting neighboring layers. Interestingly, calcined COE-5 shows enhanced catalytic performance in the acetalisation of glycerol with acetone to produce solketal, compared with COK-5.
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ChemSusChem
·
2022
·Vol. 15
·期 13
·e202200092
·
IF 9.14
Xiaohong Hu,Zhijian Li,Haiyong Wang,Haosheng Xin,Song Li,Chenguang Wang,Longlong Ma,Qiying Liu
Graphical Ring-opening : Au/ZrO 2 catalysts with excellent ring-opening hydrogenolysis ability are developed for the selective hydrogenolysis of 5-hydroxymethylfurfural and furfural to produce 2-hexanol and pentanol (1-pentanol and 2-pentanol). The Au-O−Zr interfaces with Lewis acid and metal functionalities play key roles for obtaining the target products with high yield. 2-Hexanol (2-HOL) is a versatile biomass-derived platform molecule for synthesis of liquid transportation fuels, lubricants, or detergents. Herein, a one-step preparation of 2-HOL using 5-hydroxymethylfurfural (HMF) as a substrate was reported for the first time. Several Au-based catalysts supported on different metal oxides were prepared to explore the relationship between carrier and catalytic activity. The results showed that the highest 2-HOL yield of 65.8 % was obtained at complete HMF conversion over the 5 %Au/ZrO 2 catalyst. The 5 %Au/ZrO 2 catalyst exhibited excellent durability after five consecutive recycling runs, while confirming its remarkable ring-opening hydrogenolysis on other biomass-derived furanics, furfural, with a total yield of 1-pentanol and 2-pentanol of 67.4 %. The distinguished ring-opening hydrogenolysis performance of the Au/ZrO 2 catalyst originated from a synergistic effect between the interfacial Au-O-Zr oxygen vacancies-induced Lewis acidic sites (activating C−OH/C=O bonds) and metallic Au (activating H 2 ). This work provides a possibility for producing 2-HOL from HMF with high yield, expanding the sustainable application of lignocellulosic biomass.
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PubMed
高影响因子
PLANT PHYSIOLOGY
·
2023
·
IF 7.4
Li Yuanyuan,Gao Junping,Wang Ying,Cai Jun,Wu Dousheng,Wang Long,Pu Wenxuan,Yu Feng,Zhu Sirui
Transfer RNA (tRNA) can produce smaller RNA fragments called tRNA-derived fragments (tRFs). tRFs play critical roles in multiple cellular programs, although the functional mechanisms of tRFs remain largely unknown in plants. In this study, we examined the phenotype associated with 5′ tRF-Ala (tRF-Ala, produced from tRNA-Ala) overexpression and knockdown lines (tDR-Ala-OE and tDR-Ala-kd, respectively) and the mechanisms by which tRF-Ala affects mRNA levels in Arabidopsis (Arabidopsis thaliana). We investigated the candidate proteins associated with tRF-Ala by quantitative proteomics and confirmed the direct interaction between tRF-Ala and the splicing factor SERINE-ARGININE RICH PROTEIN 34 (SR34). A transcriptome sequencing analysis showed that 318 genes among all the genes (786) with substantial alternative splicing (AS) variance in tDR-Ala-OE lines are targets of SR34. tRF-Ala diminished the binding affinity between SR34 and its targets by direct competition for interaction with SR34. These findings reveal the critical roles of tRF-Ala in regulating mRNA levels and splicing.
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PubMed
高影响因子
ACS Applied Bio Materials
·
2022
·
IF 0
Chun-Xia Zhang,Hong-Wei Li,Renwen Zhang,Zhongyuan Ren,Yuqing Wu
In the present work, cytidine 5′-monophosphate capped gold nanoclusters (AuNCs@CMP) are reported as a catalyst for redox reactions, which show both oxidase- and excellent peroxidase-like activity. When employing 3,3′,5,5′-tetramethylbenzidine (TMB) as a substrate in the presence of hydrogen peroxide (H2O2), the maximum velocity (Vmax) was 175 × 10–8 M s–1in vitro. Besides, the AuNCs@CMP exhibited high catalytic activity for reactive oxygen species (ROS) generation with H2O2. Particularly, they also displayed excellent catalytic activity for ROS generation in tumor cells, being activated and promoted by the tumor microenvironment (TME). Consequently, the AuNCs@CMP show an excellent antitumor effect on HeLa and SW480 cells as assayed by flow cytometry. The antitumor mechanism of AuNCs@CMP was attributed to the high ROS generation based on the specific environments of the TME. Therefore, the present study provides TME-adaptive AuNCs@CMP with excellent mimetic peroxidase activity, producing significant ROS to kill the tumor cells in TME.
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PubMed
ACS Omega
·
2024
·
IF 3.7
Min Zhou,Liyuan Wei,Chongzhi Wu,Wei Chen,Zhengju Tang
Cytidine 5′-monophosphate (CMP) was widely applied in the food and pharmaceutical industries. Currently, CMP is mainly produced by enzyme catalysis. However, the starting materials for enzyme catalysis were relatively expensive. Therefore, seeking a low-cost production process for CMP was attractive. In this study, Escherichia coli (E. coli) was systematically modified to produce CMP. First, a the cytidine-producing strain was constructed by deleting cdd, rihA, rihB, and rihC. Second, the genes involved in the pyrimidine precursor competing pathway and negative regulation were deleted to increase cyti dine biosynthesis. Third, the deletion of the genes that caused the loss of CMP phosphatase activity led to the accumulation of CMP, and the overexpression of the rate-limiting step genes and feedback inhibition resistance genes greatly increased the yield of CMP. The yield of CMP was further increased to 1013.6 mg/L by blocking CMP phosphorylation. Ultimately, the yield of CMP reached 15.3 g/L in a 50 L bioreactor. Overall, the engineered E. coli with a high yield of CMP was successfully constructed and showed the potential for industrial production.
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PubMed
高影响因子
ENERGY & FUELS
·
2020
·
IF 3.421
Wei Zhao,Shanshan Ruan,Siyu Qian,Beibei Feng,Chengcheng Ao,Lei Wang,Fuyi Liu,Lidong Zhang
Catalytic combustion has become a promising technology to address the accelerating volatile organic compounds (VOCs) emissions. In this work, cobalt modified ZSM-5 zeolite catalysts (3–18 wt % Co) were prepared to investigate the combustion of n-butane (one typical model component of VOCs). The performances of catalysts were evaluated by conducting experiments in a flow tube reactor. XRD, BET, FT-IR, and XPS were also utilized to characterize their physicochemical properties. A significant enhancement of the catalytic activity was demonstrated in terms of the cobalt supported over ZSM-5, with an optimal cobalt loading of 7 wt %. The excellent catalytic activity of 7 wt % Co-ZSM-5 is mainly attributed to a higher concentration of active surface Co3+ species. Furthermore, DFT calculations indicated that the role of Co can enhance the activity by decreasing the activation barriers for C–H bond cleavage. In addition, the primary reaction mechanism of n-butane combustion on Co-ZSM-5 was also revealed.
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高影响因子
JOURNAL OF CHEMICAL RESEARCH
·
2017
·
IF 0.646
Da-Hua Shi,Hui-Long Zhu,Yu-Wei Liu,Zong-Ming Tang,Chen Lu,Xiao-Dong Ma,Xiao-Kai Song,Wei-Wei Liu,Tong Dong,Meng-Qiu Song
Three novel 5-benzyl-1,3,4-thiadiazole derivatives were synthesised starting from phenylacetic acid derivatives. These compounds were characterised by NMR, HRMS and single-crystal X-ray diffraction analysis. 2-Pyrrolidyl-5-[2-(4-bromophenyl)methyl]-1,3,4-thiadiazole showed moderate acetylcholinesterase-inhibition activity with a 50% inhibitory concentration value of 33.16 μM. 2-Pyrrolidyl-5-[2-(4-bromophenyl)methyl]-1,3,4-thiadiazole and acetylcholinesterase docking was demonstrated using the Molecular Operating Environment program.
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高影响因子
RSC Advances
·
2016
·Vol. 6
·期 16
·12782-12787
·
IF 3.289
Ye-Min Lu,Hu Li,Jian He,Yan-Xiu Liu,Zhi-Bing Wu,De-Yu Hu,Song Yang
Partially hydroxylated AlF3 (denoted as AlF3) was synthesized by using a sol–gel method, and unambiguously characterized by FT-IR, XRD, NH3-TPD, SEM, TEM, N2 adsorption–desorption, and FTIR (pyridine-adsorption) techniques. The resulting mesoporous material (AlF3) simultaneously bearing Lewis and Brønsted acid sites was efficient for producing 5-hydroxymethylfurfural (HMF) from glucose, and an optimized HMF yield of 57.3% at a glucose conversion of 95.5% was obtained within 10 h at 140 °C. The effects of reaction temperature, time, catalyst amount, solvent and substrate type, and the amount of Brønsted/Lewis acid sites on conversion of glucose to HMF were also investigated. The existence of both Lewis and Brønsted acid sites in AlF3 was found to play a key role in glucose-to-HMF conversion.
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高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2020
·
IF 3.573
Jiaojiao Liu,Huifang Zhang,Ningyue Lu,Xiaoliang Yan,Binbin Fan,Ruifeng Li
A series of mesoporous ZSM-5-x (x = Si/Al molar ratio) supports, possessing various Si/Al ratios, were prepared without using the secondary templates, and the influences of acidity of ZSM-5-x on catalytic performance of Pt/HZSM-5-x samples prepared by the conventional wetness impregnation in naphthalene hydrogenation were investigated. The results show that decreasing Si/Al ratios of mesoporous ZSM-5 supports promotes the dispersion of the supported Pt species, and the catalytic efficiencies of Pt/HZSM-5-x samples for naphthalene hydrogenation depend on the integrated effects of the Pt dispersion, the acidity, and the mesoporous structure of ZSM-5 supports. This work provides the detailed influences of acidity of mesoporous ZSM-5 on catalytic performance of supported Pt catalysts in naphthalene hydrogenation.
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BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Lishi Yan,Ruoshui Ma,Huaixin Wei,Liangzhi Li,Bin Zou,Yiwen Xu
The production of 5-hydroxymethylfurfural (5-HMF) from cellulose catalyzed by a series of transition metal chlorides (i.e. FeCl 3 , RuCl 3 , VCl 3 , TiCl 3 , MoCl 3 and CrCl 3 ) was studied in biphasic system. RuCl 3 was the most efficient catalyst among these transition metal chlorides for 5-HMF production, and resulted in both the highest yield of 83.3% and selectivity of 87.5% in NaCl-aqueous/butanol biphasic system. XRD analysis and FTIR spectroscopy were applied to further characterize the RuCl 3 catalyzed cellulose slurries to reveal the catalytic reaction mechanism. Results demonstrated that RuCl 3 enhanced the decrystallization and cleavage of C O C bonds in cellulose, promoted the subsequent dehydration of glucose into 5-HMF, while suppressed the glucose retro -aldol reaction to byproduct lactic acid. In addition, with the assistance of NaCl-aqueous/butanol biphasic system, 5-HMF further degradation was limited and thusly maintained a desired 5-HMF yield. This proposed approach provides an efficient strategy for one-pot conversion of cellulose into 5-HMF.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Xitong Yang,Ruifeng Zhou,Guowei Liu,Sajjad Hussain,Qiuye Li
Catalytic regeneration has become one of the most promising technologies to solve the high energy consumption problem of CO 2 capture in organic amine solutions. As the most mainstream type of catalyst, the instability of acidic catalysts in alkaline solutions attracts increasing attention from researchers. Herein, we proposed a composite catalyst of MnOOH loaded HZSM-5 to weaken the adsorption of alkaline substances and improve the catalytic stability of the catalytic material. The optimized catalytic effect is obtained when the mass ratio of MnOOH is 20% The CO 2 desorption efficiency is increased by 68.2%, and the energy consumption is reduced by 31.8% when the dosage of the catalyst is only dosage of 0.2 wt% amine solution. Through materials characterization and DFT calculations, it was found that the surface O and hydroxyl groups on MnOOH are the key factors for proton cycling, and HZSM-5 has an important impact on improving proton transfer rate. This work provides a valuable approach to solving the stability problem of acidic catalysts.
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