ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Zifei Wu,Hao Ding,Kailai Xu,Chenghui Li,Xiandeng Hou
Background due to the toxicity and widespread distribution of inorganic mercury ions (Hg 2+ ), it is a crucial task for researchers to develop simple methods for field analysis of water samples for trace Hg 2+ . Result a colorimetric and ratiometric fluorescent dual-mode assay based on bifunctional UiO–(COOH) 2 –NH 2 /Eu and gold nanocomplexes (AuNPs@UiO–(COOH) 2 –NH 2 /Eu) was constructed for the sensitive, selective and potentially on-site determination of Hg 2+ . The incorporation of Hg 2+ resulted in the formation of gold amalgam, which enhanced peroxidase-like activity of AuNPs@UiO–(COOH) 2 –NH 2 /Eu, accelerating the oxidization of zymolyte 3,3′,5,5′-tetramethylbenzidine (TMB). Concurrently, the color of the reaction solution turned blue, and the RGB (red, green, and blue) of the solution color changed accordingly. The fluorescence at 618 nm was almost unchanged with the increase of Hg 2+ concentration, whereas the fluorescence at 460 nm was obviously quenched due to the inner filter effect (IFE). Under UV irradiation, the fluorescence emission of the sensing system changed from blue to red, enabling easy visual detection. The probe showed great linearity over a wide range of 20–1200 nM (colorimetric) and 50–4000 nM (fluorescent), with limits of detection (LODs) of 8 nM and 3 nM, respectively. Significance the proposed method was successfully applied to the determination of trace Hg 2+ in tap water and groundwater, while the use of a smartphone enables its simplicity and portability. The results show that the colorimetric and ratiometric fluorescent dual-mode method based on AuNPs@UiO–(COOH) 2 –NH 2 /Eu can achieve rapid visual determination of Hg 2+ potentially useful for on-site analysis.
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Analytical Methods
·
2025
·
IF 2.6
Yunliang Liu,Jing Gao,Yuting Huo,Yang Yang,Daoping Yue,Xianghang Lin
MiRNA-155 is a reliable biomarker for cancers and allergic rhinitis, also plays a critical role in regulating gene expression and mediating the allergic TH2 process. The accurate determination of miRNA-155 will be significant for diagnosing allergic rhinitis, especially for children. Herein, Yb-Bi2S3 nanorods were prepared through a hydrothermal method. Gold nanoparticles (AuNPs) were assembled onto Yb-Bi2S3 nanorods surface to provide a Yb-Bi2S3/AuNPs nanocomposite. Yb-Bi2S3/AuNPs nanocomposite was used as a photoactive element for fabricating a near-infrared photoelectrochemical aptasensing platform by assembling the aptamer for specific recognition of miRNA-155. The photocurrent variation of the photoelectrochemical aptasensor before and after recognizing miRNA-155 is linearly related to the miRNA-155 concentration in the range of 1.0 fmo l L-1 ~ 1.0 nmo l L-1. The detection limit was calculated to be 0.34 fmol L-1. The developed photoelectrochemical aptasensor was successfully employed for determining miRNA-155 in serum samples with satisfactory results, indicating possessing practical applications in the determination of miRNA-155 in real samples.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Zhaozhou Li,Xuebing Wei,Lijuan Wang,Ran Zhang,Huawei Niu,Jian Tao,Yafang Xie,Lele Li,Yajuan Li,Xiujin Chen,Yao Wang,Hongli Gao,Huichun Yu,Yunxia Yuan,Fang Li,Ningbo Wan,Daomin Li
An innovative streptomycin-specific electrochemical sensor was successfully established using a dual-recognition model based on the integration of molecular imprinting technology with aptamer affinity recognition. The functional interface was fabricated with a MXene/AuNPs composite covered by the specific aptamer and electropolymerized dopamine-imprinted film. Under the optimized conditions, the electrochemical sensor demonstrated a wide linear range from 1.0 × 10 −12 mol/L to 1.0 × 10 −4 mol/L with a detection limit of 0.0316 pmol/L for streptomycin. With the developed sensor, specific, rapid and accurate monitoring of streptomycin residues in milk samples was successfully achieved. The recoveries varied from 88.0 % to 105.8 % with relative standard deviations ranging from 1.7 % to 2.3 %. Overall, with the remarkable performance, the integration of MIPs with aptamer offers a novel approach to prepare the dual-recognition sensing interface and exhibits extensive adaptability and broad application prospects for the measurement, inspection and control of hazardous chemical substances in the complex matrices.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Ruonan Sun,Shuang Han,Wei Zong,Hongtao Chu,Xunan Zhang,Haiyan Jiang
The chlortetracycline (CTC) residue in food poses a threat to human health. Therefore, developing sensitive, convenient and selective analytical methods for CTC detection is crucial. This study innovatively uses tin disulfide/bimetallic organic framework (SnS 2 /ZnCo-MOF) nanocomposites in conjunction with gold nanoparticles (AuNPs) to co-modify a glassy carbon electrode (GCE). Further, a molecularly imprinted polymer (MIP)-based electrochemical sensing platform Au-MIP/SnS 2 /ZnCo-MOF/Au/GCE (AZG) was fabricated for selective CTC detection. SnS 2 /ZnCo-MOF enhanced the stability and surface area of the AZG sensor. The presence of AuNPs facilitated electron transport between the probe and the electrode across the insulating MIP layer. The fixation of AuNPs and MIP via electropolymerization enhanced the selective recognition of this sensor and amplified its output signal. The AZG sensor demonstrated a wide linear detection range (0.1–100 μM), low detection limit (0.072 nM), and high sensitivity (0.830 μA μM −1 ). It has been used for detecting CTC in animal-origin food with good recovery (96.08%–104.60%).
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 415
·期 5
·855-865
·
IF 4.478
Lai Haohong,Niu Yuanyuan,Ming Pingtao,Wang Jinhao,Wang Shumei,Xu Yan,Zhai Haiyun
In this paper, a novel electrochemical sensor was constructed for the detection of purine bases. Ultrafine carbide nanocrystals confined within porous nitrogen-doped carbon dodecahedrons (PNCD) were synthesized by adding molybdate to ZIF-8 followed by annealing. With MoC-based PNCDs (MC-PNCDs) as the carrier, gold nanoparticles (AuNPs) were deposited on the electrode surface via potentiostatic deposition as the promoter of electron transfer, forming a AuNPs/MC-PNCDs/activated glassy carbon electrode (AGCE) sensor. MC-PNCDs had a large specific surface area, which combined with the excellent electrocatalytic activity of AuNPs, synergistically improved the electrocatalytic activity. The morphology and structure of the electrode surface modifier were characterized by scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray photoelectron spectroscopy, infrared spectroscopy, X-ray diffraction, nitrogen adsorption–desorption analysis, and electrochemical characterization. Under the optimal conditions, the linear detection range of guanine (G) and adenine (A) was 0.5–160.0 μM, and the detection limits (S/N=3) were 72.1 and 69.6 nM, respectively. AuNPs/MC-PNCDs/AGCE was successfully constructed, and was used to simultaneously detect G and A with high sensitivity and selectivity. Moreover, the sensor was successfully used to detect G and A in herring sperm DNA samples. Graphical abstract
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Ziyao Mu,Peiran Sun,Pengsheng Chen,Qing Zhou,Haiyun Zhai
A sensor based on UiO-66@MWCNTs, AuNPs, and ABSA was constructed for methyl parathion detection. • UiO-66@MWCNTs provides abundant Zr OH sites for MP adsorption and preconcentration. • The sensor exhibits a wide linear range (0.1–15 μg·mL −1 ). • Satisfactory recoveries (97.43%–101.5%) were obtained in traditional Chinese herbs. A non-enzymatic electrochemical sensor for methyl parathion (MP) was developed based on UiO-66@MWCNTs/AuNPs/ABSA composites. These composites were synthesized via a solvothermal route, followed by AuNPs electrodeposition and ABSA electropolymerization. The UiO-66@MWCNTs matrix offers a large surface area and abundant binding sites for organophosphorus groups, enhancing MP adsorption and preconcentration. AuNPs effectively boost conductivity and catalytic activity, while the electropolymerized ABSA further facilitates electron transfer due to its excellent electrocatalytic properties. Thus, the composite displays remarkable electrocatalytic activity toward MP. Using the MP reduction peak current, the sensor achieves a linear range of 0.1–15 μg·mL −1 and a detection limit of 0.0152 μg·mL −1 (S/N = 3). It also exhibits good selectivity, satisfactory reproducibility (RSD 1.93%), excellent repeatability (RSD 1.50%), long-term stability (99.96% retention after 15 days), and successful application in traditional Chinese herbs such as Coptis chinensis French. and Citri Reticulatae Pericarpium .
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NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 16
·7722-7732
·
IF 3.3
Liran Tian,Xiangwei Song,Tianjiao Liu,Anfeng Li,Yang Ning,Xiuyi Hua,Deming Dong,Dapeng Liang
Based on the specific identification ability of aptamers towards a target, a combined UV-Visible with fluorescence detection method for acetamiprid was designed. In the absence of acetamiprid, AuNPs were uniformly distributed and had a good quenching ability for Rhodamine B's fluorescence at a higher NaCl concentration. In the presence of acetamiprid, a few AuNPs lost their protective effect and aggregated, meanwhile, the absorption peak of the UV-vis spectrum gradually redshifted from 520 nm to 680 nm. Additionally, the quenching effect of AuNPs on Rhodamine B was diminished when aggregation took place. Thus the presence of acetamiprid could be clearly determined using a UV-vis or fluorescence method. The combination of the two routes had a wider detection range and a detection limit as low as 0.0559 μM. This method was successfully used to determine acetamiprid in spiked lake water samples and would be a potential simple method for the determination of acetamiprid residues in natural water bodies.
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ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Bodong Wang,Shan Pu,Chengyan Xia,Xiandeng Hou,Kailai Xu
Background Recently, headspace single-drop microextraction (HS-SDME) has attracted some attention for developing sensitive and selective colorimetric assays due to its excellent capability to reduce matrix interference and enrich analytes. However, the single droplet limits direct visual observation of color change and its quantitative measurement suffers from reduced optical path length. Therefore, amplifying the detection signals in both volume and intensity is an important and challenging task for improving the sensitivity, stability, and accuracy of such colorimetric analysis . Results In this study, a "headspace-nanoenzyme" (HS-NE) strategy was proposed that successfully addressed these challenges and enabled the colorimetric and fluorescent dual-mode detection of trace Hg 2+ . Atomic Hg 0 , generated via chemical vapor generation (CVG), underwent headspace reaction with AuNPs droplet to form Au@HgNPs, thus catalyzing the oxidation of o -phenylenediamine (OPD) in the presence of H 2 O 2 . The absorbance and fluorescence intensity of oxidized OPD were proportion to the concentration of Hg 2+ in the sample solution. Due to the greatly enhanced peroxidase-like activity by Au@HgNPs, the limit of detection was as low as 0.98 nM and 0.21 nM for the colorimetric and fluorescent modes, respectively. The applicability of this assay was further demonstrated with determination of Hg 2+ in real environmental and biological samples. Moreover, a convenient and cost-effective paper-based sensing platform was fabricated for rapid on-site detection of Hg 2+ . Significance and novelty This novel HS-NE strategy combines HS-SDME and nanoenzyme-based sensing to achieve dual effects of eliminating matrix interference and amplifying the measurement signal, resulting in improved accuracy, enhanced stability, high sensitivity, and exceptional selectivity , with great potential for on-site determination of trace Hg 2+ .
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ANALYTICAL SCIENCES
·
2022
·Vol. 38
·期 9
·1181-1188
·
IF 1.967
Wang Cheng-Yan,Ge Qingmei,Jiang Nan,Cong Hang,Tao Zhu,Liu Mao,Fan Ying
Metal–organic frameworks (MOFs) including cucurbit[7]uril block (Q[7]·HAuCl 4 ) were employed to develop a diphenylamine (DPA) sensor in electrochemical method, the presence of HAuCl 4 improved the conductivity of the macrocyclic compound. To further enhance of the sensitivity, Au nanoparticles were inserted between the surface of glassy carbon electrode and Q[7]·HAuCl 4 MOFs (GCE-AuNPs-Q[7]·HAuCl 4 ). Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV) were applied for evaluation on the electrochemical behavior. For the electrochemical inertness of DPA, a label-free electrochemical sensor in 5 mM K 3 [Fe(CN) 6 ] solution was achieved, to produce a limit of detection as low as 4.6 µM in a linear range of 5–1000 µM with good reproducibility, high stability and acceptable anti-interference ability. Application of the proposed electrode for the quantitative determination of DPA in tap water and apple juice confirms its real value. Graphical abstract
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yiyun Zhang,Jie Bian,Yuxi Li,Tao Lin,Jifan Zhang,Kefeng Huo,Xunyong Liu,Yanli Liu,Yi Liu
Excessive intake or lack of iodide ions (I - ) will have serious impact on human growth and health. Therefore, the highly selective and sensitive detection of I - is particularly urgent. Herein, a dual-modal sensor based on colorimetric and gel-sol signal responses has been developed for the visual detection of I - . Visual sensing based on I - triggered dual-signal simultaneous response can reduce the interference of false positive signals and improve detection accuracy. This dual-modal sensor (Gel-AuNPs) is constructed by the in situ reduction of chloroauric acid using gelatin (Gel) with unique topological structure and reduction properties to prepare gold nanoparticles (AuNPs). The presence of I - can induce a noticeable color change from red to yellow (attributed to the occurrence of redox reaction), and the selective visual detection of I - was realized in a wide concentration range with a linear fitting coefficient of 0.9983 and a limit of detection (LOD) of 30.1 μM. Simultaneously, based on the change in viscosity in the gel-sol phase transformation process (attributed to the salting-in effect of I - ), the quantitative relationship of I - detection ( R 2 = 0.9937) was then established. Furthermore, the Gel-AuNPs hydrogel exhibited high sensitivity for detecting I - in actual water samples such as seawater and tap water. More importantly, the in situ synthesized Gel-AuNPs showed good solid-state stability and strong salt tolerance, implying the potential scope of their practical application in different harsh conditions. As such, this visual sensing system with dual signal responses will have prospective applications in detection and analysis in due course.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Haiyan Zhao,Linna Dou,Jujie Ren,Min Cui,Na Li,Xueping Ji,Xiaoting Liu,Cong Zhang
Adenosine triphosphate (ATP), as the primary energy source and significant biomarker, is associated with the occurrence and progression of various diseases. Therefore, detecting ATP sensitively is essential for ATP-related biochemical study, disease diagnosis and treatment. Herein, sensitive electrochemical aptasensing of ATP was proposed based on MOF-derived porous Co 3 O 4 coupled with AuNPs and nucleic acids as a novel electrocatalysis signal probe . Firstly, [email protected] 3 O 4 nanocomposite was synthesized via pyrolysis Co-zeolitic imidazolate framework and then functionalized with AuNPs by in situ chemical reduction, which showed excellent electrocatalytic activity towards hydroquinone (HQ) as well as excellent stability. Then, DNA capture probe (CP) was connected to [email protected] 3 O 4 nanocomposite via Au-S bond to form [email protected] 3 O 4 -CP signal probe for signal transduction . Meanwhile, well-designed aptamer probe (AP) containing ATP aptamer and its complementary sequence was immobilized on AuNPs modified electrode surface via Au-S bond. In the presence of ATP, the aptamer in AP interacted with ATP, resulting in the dissociation of AP. Consequently, [email protected] 3 O 4 -CP signal probe could be captured on electrode surface via hybridization, leading to a significant amplified electrocatalytic signal towards HQ. Finally, the quantitative determination of ATP was achieved by determining the current signal generated on electrode surface. The proposed electrochemical aptasensor demonstrated excellent sensitivity and selectivity that could detect ATP as low as 0.3 nM. Moreover, the aptasensor showed excellent performance for detecting ATP in complex serum matrix. These results indicate that MOF-derived nanocomposites have promising application in construction of novel electrocatalysis signal probe as well as versatile bioassays.
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ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yu Liu,Yanan Cao,Cong Zhang,Cheng Ye,Qinglai Bian,Xue Cheng,Hui Xia,Junping Zheng,Hongtao Liu
The inhibition of hydrogen peroxide (H 2 O 2 ) on the cysteine-mediated aggregation of gold nanoparticles (AuNPs) has great potential to rapidly visualize H 2 O 2 and disease-associated biomarkers. However, associated applications have been rigid due to the low cysteine-oxidation efficiency or the insufficient interference-resistance of previous catalysts. Here, we proposed a novel AuNPs colorimetric method termed Fe 2+ -catalyzed H 2 O 2 -preventing aggregation of AuNPs by oxidizing Cysteine (FeHOAuC) for the visible and rapid detection of H 2 O 2 with high specificity. Formed Fe 2+ -cysteine composites in the beginning, Fe 2+ accelerates the oxidation of cysteine by H 2 O 2 with an intramolecular electron-transferring model, and the generated cysteine oxides (including cysteic acid or cystine , dependent on the ratio of cysteine to H 2 O 2 molecules) lose the pro-aggregation effect on AuNPs. Notably, FeHOAuC can quantify 2–30 μM of H 2 O 2 within 5 min, and the monitoring process works well with tolerance to the impact of dissolved oxygen. The FeHOAuC paired with lactate oxidase was successfully used for measuring lactic acids in real sweat samples with a practicable detection window (2–60 μM) and a low variable coefficient (<10%). In summary, FeHOAuC is a feasible platform for rapid and convenient detection of H 2 O 2 and oxidase-specific substrates.
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