3 Biotech
·
2021
·Vol. 11
·期 4
·1-9
·
IF 2.406
Ren Runxian,Zhai Lixin,Tian Qiaopeng,Meng Di,Guan Zhengbin,Cai Yujie,Liao Xiangru
Diphenyl phosphate (DPHP) has been increasingly detected in environmental samples, posing a potential hazard to humans and other organisms and arousing concern regarding its adverse effects. Biological degradation of DPHP is considered a promising and environmentally friendly method for its removal. In this study, the bagdpd gene was mined from the Bacillus altitudinis W3 genome and identified as a glycerophosphodiester phosphodiesterase by bioinformatics analysis. The enzyme was expressed and its biochemical properties were studied. When using bis(4-nitrophenyl) phosphate as substrate, enzyme activity was optimal at 55 °C and a pH of 8.5. The enzyme remained stable in the pH range of 8.0 − 10.0. The rBaGDPD enzyme degraded DPHP and the reaction product was identified as phenyl phosphate by LC–MS. This is the first report of a glycerophosphodiester phosphodiesterase exhibiting hydrolytic activity against DPHP. This study demonstrated that rBaGDPD could have the potential for bioremediation and industrial applications.
研究领域:
环境科学
·
微生物学
·
生物化学
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PubMed
高影响因子
ChemistrySelect
·
2025
·Vol. 10
·期 39
·e04041
·
IF 2
Xintong Huan,Mingxin Jiang,Can Gao,Meixuan Liu,Xiaohuan Sun,Jie Han
Enantioselective sensing of 3,4-dihydroxyphenylalanine (DOPA) enantiomers is highly significant in biology and pharmaceutics, yet developing sensors with high enantioselectivity and analyte-specific output signals remains challenging. In this study, we constructed an L-cysteine (Cys)-Fe 2+ nanozyme through self-assembly of L-Cys and Fe 2+ . This nanozyme exhibited efficient peroxidase-like activity, with a K cat value of 2.1 × 10 −9 M·s −1 ·µg −1 toward 3,3“,5,5”-tetramethylbenzidine (TMB). When using D/L-DOPA as a substrate, we observed higher catalytic efficiency for L-DOPA than for D-DOPA, with a selectivity factor of 1.6. Mechanistic studies revealed that this enantioselectivity originates from the preferential adsorption of D-DOPA over L-DOPA on the nanozyme surface, which impedes the oxidation kinetics by blocking substrate access to the active sites. These chirality-specific changes induced in UV–vis absorption during catalysis enabled the establishment of analyte-specific calibration curves for D-DOPA, L-DOPA, and enantiomeric compositions in D/L-DOPA mixtures, achieving highly enantioselective sensing of DOPA enantiomers. This sensing platform demonstrated high sensitivity (limit of detection: 3.6 µM), excellent selectivity, and significant potential for practical applications. These findings provide novel insights for developing superior enantioselective sensors with high accuracy. Graphical Through the self-assembly of L-cysteine (Cys) and Fe 2+ , we constructed a novel chiral L-Cys-Fe 2+ nanozyme. This nanozyme achieved high enantioselectivity in discriminating 3,4-dihydroxy-D/L-phenylalanine (D/L-DOPA) due to enantioselective adsorption. This approach constitutes a promising new platform for reliable enantiomer detection.
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产品关联
DOI
高影响因子
ACS Applied Optical Materials
·
2025
·
IF 3.8
Zhao-Xing Jin,Hong-Sheng Gao,Xiao-Qiang Huang,Zhi-Hui Ren,Zheng-Hui Guan
Luminescent organic molecules play a pivotal role in the advancement of organic light-emitting diode (OLED) materials. Herein, a class of acenaphthylene-cored D–π–A-type molecules were designed and synthesized (isolated yields >85% for each step), wherein the acenaphthylene served as a conjugated bridge integrating a benzoxazole acceptor moiety and a triarylamine donor unit. Computational results showed a spatially segregated distribution of the frontier molecular orbitals, which achieved a small singlet–triplet energy gap (ΔEST = 0.1 eV). The corresponding OLED device emitted green light at 543 nm. Furthermore, this work represents the first combined application of acenaphthylene and benzoxazole in luminescent organic materials.
研究领域:
材料科学
·
有机电子学
·
光电功能材料
·
理论与计算化学
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DOI
高影响因子
MATERIALS SCIENCE FORUM
·
2010
·
IF 0
Xin Wang,Yuan Yuan Dou,Mei Shan Pei,Xin De Tang
Biocompatible and biodegradable synthetic materials have attracted considerable attention during the past two decades. In this work, a series of amphiphilic triblock copolymers containing D-galactopyranose were synthesized by atom transfer radical polymerization (ATRP). The macroinitiator was prepared by the esterification between poly(ethylene glycol) monomethyl ether with a number-average molecular weight of 1200 g/mol (MeOPEO-1200) and 2-bromoisobutyryl bromide. The sugar-bearing monomer, 6-O-methacryloyl-1,2;3,4-di-O-isopropylidene-D-galactopyranose (MAIPG) was polymerized using PMDETA/CuBr as catalytic system and anisol as solvent. The chemical structure and composition of the resultant polymer MeOPEO-b-PMAIPG were verified by 1H NMR. The molecular weights and their polydispersities were characterized by gel permeation chromatography (GPC). The results indicate that the polymerization follows the mechanism of ATRP. The amphiphilic copolymers can self-assembly to from micelles with PMAIPG as the core and PEO as the corona, which have potential applications as biomaterials or controlled release drug delivery systems.
研究领域:
高分子化学
·
生物医学工程
·
药物递送
·
材料科学
·
大分子化学
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DOI
IEEE SENSORS JOURNAL
·
2024
·Vol. 24
·期 13
·21276-21285
·
IF 4.3
Xinghua Shi,Anxue Zhang,Guoqing Han,Yuemeng Yin,Wenchao Chen
Highway bridges are primarily reinforced concrete structures. They have multilayer steel mesh sheets, corrugated pipes, and steel strands inside. As a result, detecting defects in bridge concrete structures requires high technical expertise and can be challenging. This article presents a new device of ground-penetrating radar (GPR) designed specifically for highway bridge inspection. It employs antenna array technology, multichannel radar control technology, and real-time 3-D data acquisition and display technology to achieve reliable detection of various concrete structures commonly found in bridges. Using a 1.3 GHz central frequency antenna array and a multichannel control unit, the system enables simultaneous surveying along five lines, the detection depth can exceed 600 mm in concrete. This significantly enhances detection efficiency. By employing specialized software processing, the detection results are presented in a specific and visually comprehensive 3-D format. Moreover, the system provides 3-D slice images from any position and direction, facilitating the interpretation of detection results for various targets, including reinforcement layout, protective layer thickness, corrugated pipes, steel strands, and other quality defects. It provides advanced and reliable technical means for construction quality control and bridge health monitoring of highway bridges. The results indicate that the device performs well not only in controlled experimental environments, but also in real-world bridge structure environments.
研究领域:
土木工程
·
结构健康监测
·
无损检测
·
交通基础设施
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DOI
高影响因子
Nature Chemical Biology
·
2026
·
IF 15.8
Liu Jiapeng,Han Jian,Wang Yupu,Song Mengjiao,Zhu Jianbei,Liang Yu,Chen Fangyi,Liu Zhike,Yan Xinwen,Wang Ziyuan,Peng Weicheng,Tu Ruibo,Zhang Zihan,Zhong Binglin,Sun Hao,Yin Junyi,Chang Jingbo,Long Yuying,Men Yinghui,Tsai Shang-Jui,Wang Shanshan,Susanto Teodorus Theo,Qiang Albert,Chen Yong,Pan Weijun,Lin Yizhu,Wu Wei,Shen Yidong,Li Jiefu,Wan Yue,Han Shuo
Understanding the spatial organization of biomolecules in living organisms is a central but challenging goal in biology. Here, we introduce peroxidase reactions activated by D -amino acids (PRADA), which uses an engineered oxidase to convert nonproteinogenic D -amino acids into H 2 O 2 for in situ activation of a genetically fused peroxidase. PRADA enables versatile proximity-based chemistries, including protein labeling, RNA labeling and functional polymer assembly, with minimal toxicity and background. We demonstrate that PRADA is broadly applicable inside living organisms, including flies, worms, fish and mice. By further leveraging the unique RNA reactivity of PRADA, we developed a mutational profiling sequencing strategy for spatiotemporally resolved mapping of RNA secondary structure. Application of this multifaceted PRADA platform in a mouse xenograft model offered insights into its mitochondrial proteome, transcriptome and RNA structurome, revealing RNA folding as a regulatory mechanism in mitochondrial gene expression. Collectively, PRADA holds promise to uncover the intricate organization of biomolecules in vivo.
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DOI
高影响因子
Journal of Functional Foods
·
2026
·
IF 3.9
Ming Nie,Chenhao Liu,Xiaozhen Gu,Hui-Li Wang
Peony seed oil attenuated selected behavioral deficits in D-gal-treated male mice. • PSO-treated fields showed qualitatively more orderly hippocampal morphology. • PSO lowered serum MDA at all doses; SOD increased only at the medium dose. • PSO altered hippocampal synapse-related mRNAs in a dose-specific manner. Peony seed oil (PSO) is rich in unsaturated fatty acids, but its effects on cognitive impairment remain unclear. Male C57BL/6J mice received D-galactose (200 mg/kg/day) with or without PSO (2.4, 4.8, or 9.6 mL/kg/day) for 8 weeks. Cognition, hippocampal morphology, serum malondialdehyde and superoxide dismutase, hippocampal synapse-related transcripts, PSO composition, and predictive network pharmacology were assessed. PSO improved Morris water maze and Y-maze performance and was associated with more preserved hippocampal morphology. It also improved serum oxidative stress-related indicators and upregulated hippocampal Dlg4 , Syn1 , Slc17a7 , Grin1 , Grin2b , and Stx1a mRNA. PSO contained 45.60% alpha-linolenic acid, 22.62% linoleic acid, and 17.12% oleic acid. Network analysis generated unvalidated candidate targets and pathways. PSO attenuated D-galactose-induced cognitive deficits, although protein-level, brain oxidative-stress, and mechanistic validation is still required. Download: Download high-res image (163KB) Download: Download full-size image
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高影响因子
WATER RESEARCH
·
2026
·
IF 12.8
Jiajun Lu,Rongjie Jiang,Defang Ma,Baoyu Gao,Shuang Zhao,Yan Wang
Oxidative polymerization driven by direct electron transfer (DET) demonstrates superior selectivity and strong anti-interference capability in water purification. However, its practical application is constrained by inherent limitations, including inadequate polymerization efficiency and extent, as well as catalytic site deactivation. To address these challenges, we propose a catalytic pathway engineering strategy through d-band broadening. Building upon this principle, a cobalt-manganese oxide-modified graphite felt (Co‑MnO 2 @GF) catalyst was developed and integrated into a dual-chamber reaction system. Facilitated by a downward shift of the d-band peak, surface-confined oxidative polymerization converted >94% of model pollutants into separable, potentially recoverable polymers. This yielded an effluent free of detectable intermediates and achieved near-complete removal of total organic carbon (TOC). More importantly, the electron delocalization induced by broadened d-band enables electrons derived from pollutants to migrate rapidly toward the oxidant instead of being trapped in Mn d-orbitals. This mitigates the deep reduction of Mn(IV) caused by excessive electron accumulation on Co‑MnO 2 @GF, thereby stabilizing high-valence Mn species during operation and underpinning the superior long-term stability of the catalyst in oxidative polymerization. Our work demonstrates that rational electronic structure design can effectively overcome the intrinsic barriers of DET-driven oxidative polymerization, establishing a scalable purification strategy for sustainable water treatment.
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Ling Li,Lichen Gou,Kaixi Yin,Xinni Ng,Longcheng Zhang,Xiandeng Hou,Li Wu
Aromatic organic pollutants pose severe risks to aquatic ecosystems and call for analytical strategies that couple trace-level detection with efficient in situ degradation. Here, we report a plasmonic nanozyme composed of Ag nanoparticles on metal-organic framework-derived Fe 2 P (Ag@Fe 2 P/C), in which the Ag-Fe 2 P heterointerface integrates surface-enhanced Raman scattering (SERS) sensing with Fenton-like oxidation. Ag@Fe 2 P/C enabled ultrasensitive, quantitative SERS detection of crystal violet, 4-nitrophenol, and bisphenol A, with limits of detection of 10⁻ 10 –10⁻ 8 M, correlation coefficients R 2 above 0.95, and relative standard deviations below 5%. Under mild H 2 O 2 treatment, Ag@Fe 2 P/C completely degraded crystal violet within 10 min and 4-nitrophenol and bisphenol A within 30 min, and fully recovered its initial SERS response over six regeneration cycles. Density functional theory calculations show that the Ag-Fe 2 P heterointerface shifts the d band of Fe sites and enhances interfacial electron transfer, which in turn accelerates the Fe 2 ⁺/Fe 3 ⁺ redox cycle and promotes H 2 O 2 activation into hydroxyl radicals that remove adsorbed pollutants and regenerate SERS-active sites. By bridging plasmonic enhancement with enzyme-mimetic catalysis through interface engineering, this work provides a promising strategy to enable in situ SERS monitoring and degradation of representative aromatic organic pollutants.
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高影响因子
Materials Horizons
·
2026
·
IF 11.4
Xin Hu,Shengyang Wang,Xiaoping Tao,Ruizhi Duan,Qi Ye,Jun Li,Zhaochi Feng,Mingge Zhai,Can Li
Efficient photothermal conversion relies on the dissipation of energy from photoexcited charge carriers through non-radiative pathways. However, the fundamental electronic structure origins governing such processes in semiconductors remain poorly understood, limiting the rational design of photothermal materials. Here, using TiO2 as a model system, we demonstrate that multi-transition-metal doping provides an effective strategy to simultaneously regulate electronic configurations and spin states, thereby governing carrier relaxation pathways. The incorporation of Fe, Co, and Mn into the TiO2 matrix induces a redistribution of 3d-orbital defect states within the bandgap, giving rise to d–d interband transitions (IBTs), suppression of spin polarization, and accelerated non-radiative recombination. As a result, the optimized FeCoMn–TiO2 exhibits broadband solar absorption and a near-unity photothermal conversion efficiency (97.9%), along with a high solar steam generation rate of 3.82 kg m−2 h−1 (91.2%). These findings establish a direct link between d-orbital electronic structure, spin characteristics, and non-radiative energy dissipation, providing a conceptual framework for designing photothermal semiconductors through electronic state engineering.
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DOI
PubMed
高影响因子
Molecular Catalysis
·
2025
·
IF 4.9
Xiaojuan Yu,Zili Zhang,Wenlong Lu,Qian Li,Chenghua Gao,Cefan Zhou,Jingfeng Tang,Zhiyong Guo,Aitao Li
Steroids are important pharmaceutically active compounds, and cytochrome P450 monooxygenases (CYPs) serve as promising biocatalysts for steroidal drug synthesis. However, their application is often limited by low catalytic efficiency and incomplete understanding of regioselectivity mechanisms, despite their remarkable oxygen activation capabilities. In this study, we engineered P450BM3 to enable high-efficiency C15α-hydroxylation of d -ethylgonendione, producing a key intermediate in the synthesis of gestodene, a widely used progestogen hormonal contraceptive. Initial screening of a laboratory P450 library identified variant HG12 (P450BM3/F87G/A328G/A330W), which exhibited 54.1 % regioselectivity and 45.8 % substrate conversion. Subsequent semi-rational design targeting residues lining in the active pocket and substrate channel, variant HG12M8 (R47W/F87G/M185L/A328G/A330W/T438G) was obtained, achieving 89.5 % regioselectivity and 90.1 % conversion. Molecular dynamics simulations revealed that F87G and M185L mutations alleviate steric hindrance and stabilize substrate binding, while R47W widens the substrate access channel. This work presents a dual-purpose protein engineering strategy to overcome efficiency and selectivity challenges in P450s while providing a robust biocatalyst for sustainable steroidal pharmaceutical production.
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DOI
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2024
·
IF 3.3
Hui Xu,Zhi-Jiang Jiang,Yun Jia,Yuhang Su,Jian-Fei Bai,Zhanghua Gao,Jia Chen,Kun Gao
Aromatic sulfones are the prevailing scaffolds in pharmaceutical and material sciences. However, compared to their widespread application, the selective deuterium labeling of these structures is restricted due to their electron-deficient properties. This study presents two comprehensive strategies for the deuteration of aromatic sulfones. The base-promoted deuteration uses DMSO-d6 as the deuterium source, resulting in a rapid H/D exchange within 2 h. Meanwhile, a silver-catalyzed protocol offers a much milder option by using economical D2O to furnish the labeled sulfones.
研究领域:
药物化学
·
材料科学
·
有机合成
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DOI
PubMed
高影响因子
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