BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Jie-Qiong Chen,Shi-Fan Xue,Zi-Han Chen,Shengqiang Zhang,Guoyue Shi,Min Zhang
A novel label-free ratiometric fluorescent probe is developed for the detection of H 2 O 2 based on GelRed/[G 3 T] 5 /Tb 3+ hybrid, in which GelRed (a nucleic acid dye) intercalated into the designed single-stranded DNA [G 3 T] 5 (i.e. GelRed/[G 3 T] 5 ) acts as a stable build-in reference with red emission and Tb 3+ sensitized by [G 3 T] 5 (i.e. [G 3 T] 5 /Tb 3+ ) as a sensitive response signal with green fluorescence. With the successive addition of Hg 2+ and cysteine (Cys) to GelRed/[G 3 T] 5 /Tb 3+ , the fluorescence of [G 3 T] 5 /Tb 3+ can be effectively quenched and recovered respectively, while the fluorescence of GelRed/[G 3 T] 5 remains unchangeable. Combined with these properties, we have demonstrated its application for label-free ratiometric fluorescence detection of H 2 O 2 with self-calibration. The sensing mechanism is based on the specific reaction between H 2 O 2 and Cys, the resulting disulfide reverses the Cys-mediated fluorescence changes of [G 3 T] 5 /Tb 3+ . This method is further applied to the monitoring of oxidase-related reactions. As the glucose oxidase (GOx)-biocatalyzed oxidation of glucose and the acetylcholine esterase/choline oxidase (AChE/ChOx) cascade yield H 2 O 2 , such two biocatalytic processes are successfully examined utilized our proposed method, indicating its potential broad applications in biomedical analysis. In addition, we have also demonstrated its feasibility for oxidase-based visual biosensing, in which only a handheld UV lamp is used. Importantly, coupled with our proposed visual biosensing, a realtime quantitative scanometric assays has been also demonstrated by using a smartphone with easy-to-access color-scanning APP as the detection platform. Compared to traditional methods, the proposed design is cost-effective, simple to prepare and easy-to-use without fluorescence labeling or chemical modification.
研究领域:
分析化学
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生物医学工程
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生物化学
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传感器与执行器
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 2
·1-12
·
IF 5.3
Ma Shuyan,Wei Chenjie,Bao Yuheng,Liu Yanhui,Jiang Hong,Tong Weijun,Chen Dajing,Huang Xiaojun
A novel strategy based on gradient porous hollow fiber membrane (GPF) is proposed for the modular assembly of enzyme-nanozyme cascade systems. The porous structure of GPF provided sufficient specific surface area, while the gradient structure effectively minimized the leaching of enzymes and nanozymes. To enhance stability, we prepared and immobilized metal–organic framework (MOF) nanozymes, resulting in the fabrication of GPF-MOF with excellent stability and reusability for colorimetric H 2 O 2 detection. To improve specificity and expand the detection range, micro-crosslinked natural enzymes were modularly assembled, using glucose oxidase as the model enzyme. The assembled system, GPF-mGOx@MOF, achieved a low detection limit of 0.009 mM and a linear range of 0.2 to 11 mM. The sensor retained 87.2% and 80.7% of initial activity after being stored for 49 days and 9 recycles, respectively. Additionally, the reliability of the biosensor was validated through glucose determination of human blood and urine samples, yielding comparable results to a commercial glucose meter. Graphical Abstract
研究领域:
生物传感器
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纳米技术
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分析化学
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生物医学工程
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PubMed
高影响因子
Journal of Materials Chemistry C
·
2022
·Vol. 10
·期 18
·7227-7240
·
IF 8.067
Xinmeng Zhang,Zixuan Mao,Wanyin Ge,Jiajing Zhu,Yuanxiao Zhao
Surface microstructure regulation is an effective way to enhance the performance of sensing materials. Here, a hierarchical and flexible Brussels sprout-like Ni–Co(OH)2/rGO/carbon cloth (CC) composite was constructed by optimizing the morphology of Ni–Co(OH)2 microspheres and the addition amount of rGO. This sensor shows excellent reproducibility, high selectivity, and good stability. It shows a high sensitivity of 3.7391 mA mM−1 cm−2 in the 0.0388–124.0436 mM line detection range for H2O2 and 1.8457 mA mM−1 cm−2 in the 0.0300–2.0000 mM line detection range for glucose. The low limits of detection for H2O2 and glucose are 2.316 nM and 0.115 μM, respectively. The excellent electrochemical performance of the Ni–Co(OH)2/rGO/CC composite is attributed to its unique Brussels sprout-like microstructure. By studying the electrical conductivity, electrochemical active surface area, and hydrophilic–hydrophobic surfaces of the Ni–Co(OH)2/rGO/CC composite, the structure–performance relationship between the microstructure and sensing performance of the composite was further clarified. Moreover, the mechanism of CC, rGO, and the Ni–Co(OH)2 ternary composite in the sensing process is proposed. This study provides a new strategy for the construction of flexible wearable electrodes with high sensitivity.
研究领域:
电化学
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材料科学
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传感器
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纳米技术
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Kailian Zhang,Man Zhou,Kai Yang,Changlin Yu,Ping Mu,Zhenzhen Yu,Kangqiang Lu,Weiya Huang,Wenxin Dai
The low-usage of solar energy and the sluggish separation efficiency of the photogenerated electrons/holes pairs are the obstacles in the practical application of photocatalysts. The integration of upconversion and Z-scheme heterojunction is expected to break the barriers to achieve the efficient charge separation and broaden near-infrared light absorption . Herein, an effective indirect Z scheme AgInS 2 /In 2 S 3 heterostructure with carbon quantum dots (CQDs, as the electron conduction medium) and Lu 3 NbO 7 :Yb, Ho (as upconversion function) has been successfully synthesized. Consequently, the Lu 3 NbO 7 : Yb, Ho/CQDs/AgInS 2 /In 2 S 3 heterostructure exhibited superior photocatalytic activities for Cr(VI) reduction and H 2 O 2 production, reducing 99.9% of Cr(VI)(20 ppm, 15 min) and 78.5% of Cr(VI) (40 ppm, 30 min) with visible light irradiation as well as 94.0% of Cr(VI) (20 ppm, 39 min) under NIR light irradiation. Simultaneously, the heterostructure could generate 902.9 μM H 2 O 2 for 5 h under visible light irradiation. The intensive photocatalytic properties could primarily be attributed to the boosted light absorption capacity, the improved solar-to-energy conversion by the remarkable upconversion capacity of Lu 3 NbO 7 : Yb, Ho/CQDs and the faster charge transfer through a Z-schematic pathway. This work is anticipated to open a novel “window” for designing the efficient photocatalysts by coupling of Lu 3 NbO 7 : Yb, Ho and CQDs.
研究领域:
材料科学
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环境科学
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光化学
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纳米技术
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PubMed
高影响因子
PLANT JOURNAL
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2023
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IF 7.2
Xiangyang Li,Linru Chen,Xiaoyue Zeng,Kaixin Wu,Jiayu Huang,Mengmeng Liao,Yue Xi,Guohui Zhu,Xiuying Zeng,Xuewen Hou,Zhisheng Zhang,Xinxiang Peng
SUMMARY Sensing of environmental challenges, such as mechanical injury, by a single plant tissue results in the activation of systemic signaling, which attunes the plant's physiology and morphology for better survival and reproduction. As key signals, both calcium ions (Ca 2+ ) and hydrogen peroxide (H 2 O 2 ) interplay with each other to mediate plant systemic signaling. However, the mechanisms underlying Ca 2+ -H 2 O 2 crosstalk are not fully revealed. Our previous study showed that the interaction between glycolate oxidase and catalase, key enzymes of photorespiration, serves as a molecular switch (GC switch) to dynamically modulate photorespiratory H 2 O 2 fluctuations via metabolic channeling. In this study, we further demonstrate that local wounding induces a rapid shift of the GC switch to a more interactive state in systemic leaves, resulting in a sharp decrease in peroxisomal H 2 O 2 levels, in contrast to a simultaneous outburst of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-derived apoplastic H 2 O 2 . Moreover, the systemic response of the two processes depends on the transmission of Ca 2+ signaling, mediated by glutamate-receptor-like Ca 2+ channels 3.3 and 3.6. Mechanistically, by direct binding and/or indirect mediation by some potential biochemical sensors, peroxisomal Ca 2+ regulates the GC switch states in situ , leading to changes in H 2 O 2 levels. Our findings provide new insights into the functions of photorespiratory H 2 O 2 in plant systemic acclimation and an optimized systemic H 2 O 2 signaling via spatiotemporal interplay between the GC switch and NADPH oxidases.
研究领域:
植物生物学
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分子生物学
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细胞信号
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生物化学
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Zhenglin Chen,Guangzhen Liu,Shuiping Yu,Lixia Yang,Lingyi Zheng,Zhihui Wei,Shenglian Luo
Cathode materials with high hydrogen peroxide (H 2 O 2 ) selectivity and Fe 2+ regeneration under macroneutral conditions are the essential for practical electro-Fenton processes. Herein, we develop an amorphous cobalt sulphide (CoSx(t = x)@Cu) cocatalytic electro-Fenton (EF) system capable of effectively degrading organic pollutants, such as sulfamethoxazole, at macroneutral pH conditions. Owing to the flexible electronic properties of amorphous structures and charge redistribution triggered by S defects, the optimum CoSx(t = 8)@Cu cathode successfully introduces H*/H + and promotes the in-situ evolution of H 2 O 2 . Additionally, an acidic microenvironment was constructed on the CoSx(t = 8)@Cu surface by the unreduced H*/H + , enabling a self-adjustment from alkalinity to acidity in the EF reaction without adding acid. Such self-adjustment effectually inhibits iron sludge production and promotes Fe 2+ regeneration, assuring 80% Fe 2+ cycling efficiency. More importantly, the EF system possesses outstanding stability and applicability for organic pollutant remediation regardless of water matrices. This work elucidates the intrinsic mechanism of amorphous electrocatalysts in overcoming the pH limitation of EF reactions, extending the applicability of Fenton technology.
研究领域:
环境科学
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化学工程
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材料科学
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水处理
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DOI
高影响因子
Biomedical Materials
·
2024
·
IF 3.9
Xiaolu Chen,Dawei Yan,Hao Deng,Hulin Yang,Suping Peng,Wei Zhang,Shijie Cai,Qiyi Zhang,Haohao Ren,Yonggang Yan
Stopping postoperative soft tissue adhesions is one of the most challenging clinical problems that needs to be addressed urgently to avoid secondary injury and pain to patients. Currently, membrane materials with anti-protein adsorption and antibacterial activity are recognized as an effective and promising anti-adhesion barrier to prevent postoperative adhesion and the recurrent adhesion after adhesiolysis. Herein, poly(amino acid) (PAA), which is structurally similar to collagen, is selected as the membrane base material to successfully synthesize PAA-5 membranes with excellent mechanical and degradation properties by in-situ melt polymerization and hot-melt film-forming technology. Subsequently, CuSO4/H2O2 induced co-deposition of the polydopamine/polysulfobetaine methacrylate (PDA/PSBMA) coatings brought excellent biological properties to the PAA-5 membranes. In vitro studies indicated that the introduction of copper ions and quaternary ammonium salts resulted in superior hemocompatibility, anti-protein activity and cytocompatibility of PDC-5S and PDC-10S. Additionally, PDC-5S and PDC-10S demonstrated significant antibacterial activity against E. coli and S. aureus, with an inhibition rate of more than 90%. As a result, this study sheds light on newly discovered PAA membranes with anti-protein adsorption and antibacterial activity can sever as one of the promising candidates for the prevention of postoperative peritoneum adhesions.
研究领域:
材料科学
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生物医学工程
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药剂学
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PubMed
高影响因子
Acta Biomaterialia
·
2022
·
IF 10.633
Furong Liu,Tao He,Songlin Gong,Meiling Shen,Shuang Ma,Xianzhou Huang,Lu Li,Li Wang,Qinjie Wu,Changyang Gong
Combining the internal force-driven chemodynamic therapy (CDT) and the external energy-triggered photodynamic therapy (PDT) holds great promise to achieve an advanced anticancer effect based on reactive oxygen species (ROS). However, the insufficient oxy-substrates supply in tumor microenvironment, like hydrogen peroxide (H 2 O 2 ) and oxygen (O 2 ), is the Achilles heel that greatly restricts the efficacy of this ROS-based treatment. Herein, the construction of a c opper p e roxide-based tumor pH-re s ponsive a utocatalytic nano r eactor (CESAR), via an albumin-mediated biomimetic mineralization strategy is described. The decoration of human serum albumin endows the nanoreactor good hydrophilicity and biocompatibility, which is highly desired for the metal-based materials. Upon exposure to acidic tumor microenvironment, CESAR presents a pH-triggered disintegration with Cu 2+ , H 2 O 2 and O 2 generated instantly. The generated H 2 O 2 complements the hyperoxide deficiency and initiates a localized Fenton-like reaction with the assistance of Cu 2+ for highly toxic hydroxyl radicals (•OH) production for improving CDT. The evolved O 2 gas enables hypoxia relief for enhanced Ce6-mediated PDT. This H 2 O 2 /O 2 self-supplying strategy significantly amplifies the tumor oxidative damage and gains an optimal treatment outcome, which offers a new paradigm for optimizing the tumor therapeutic options limited by oxide or hyperoxide deficiency, not only for CDT/PDT, but also other oxy-substrates involved strategies. Statement of significance The shortage of oxy-substrates in the tumor microenvironment remains a great challenge for ROS-based cancer therapy. Herein, we introduce human serum albumin as a scaffold to stabilize copper peroxide nanomaterials for constant production of H 2 O 2 and O 2 to enhance chemodynamic/photodynamic therapy. The tumor pH-triggered H 2 O 2 /O 2 production and Cu 2+ release are confirmed, assuring the strategy of a highly precise, effective way to destroy tumor without any side effects. This work lends new and exciting insights into the engineering design of autocatalytic oxy-substrates self-supply nanoreactor for overcoming the bottlenecks, like the oxy-substrates deficiency of CDT/PDT and the poor stability of metal peroxides, to achieve highly effective chemodynamic/photodynamic therapy.
研究领域:
纳米技术
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癌症治疗
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生物医学工程
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材料科学
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化学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2022
·
IF 7.139
Dengyu Fu,Yanji Zhu,Lei Chen,Yue Sun,Meng Zhang,Huaiyuan Wang
Heteroatom modified catalyst applying for the two-electron oxygen reduction reaction has the potential application for efficient on-site production of H 2 O 2 . At present, state of the art catalyst synthesis is often energy-intensive and tends to destroy the original structure of heteroatoms. Based on the above considerations, Co-O, pyrrolic N structures co-modified graphite felt catalyst (Co-PDA-GF) is successfully synthesized by a simple two-step electrochemical method . Interestingly, the introduction of Co 2+ during the dopamine (DA) polymerization process changes the surface morphology of GF from a “thin-film” like to an “ant-nest” like. The cumulative H 2 O 2 concentration achieves 421 mg L −1 in 6 h test time, which is 16 times higher than that of R-GF, and the Methylene blue (MB) in-situ removal rate reaches 97.2% within 3 h. The enhanced H 2 O 2 yield of Co-PDA-GF catalyst is attributed to the increased oxygen-containing functional groups (-COOH, -C-O-C) and defect structures , and most importantly, Co-O and pyrrolic N synergistic modulation effects on local electronic modulation. This work provides new insights on DA polymerization morphology control and designing of high-efficient 2e − ORR catalyst with a low-energy method.
研究领域:
电化学
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材料科学
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催化
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环境化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Wudan Cai,Shuai Chen,Xiao Zhang,Dan Wu,Xinyi Zhu,Honglin Zhu,Yangchao Luo,Qilin Huang
The current study aimed to investigate the effect of ultrasound assisted H 2 O 2 /Vc degradation on the digestive characteristics of Lignosus rhinocerotis polysaccharide (LRP) and gut microbiota regulation. Results showed that during in vitro digestion, no changes were observed in molecular weight ( M w ) of LRP and its antitumor activity remains stable. However, M w of degraded LRP (DLRP) slightly decreased from 45.66 × 10 4 Da to 36.61 × 10 4 Da with no free monosaccharides released, enhancing its antitumor activity. The supplementation with LRP or DLRP significantly elevated mice fecal water content and modulated microbiota composition. The total short chain fatty acids concentration of control, LRP, and DLRP groups were 20.07, 24.13, and 28.08 mmol/L, respectively. Compared with LRP, DLRP significantly increased the abundance of beneficial bacteria such as Alloprevotella and Mucispirillum , exhibiting protective effects on intestinal health. This study provides a theoretical foundation for targeted regulation of intestinal flora by LRP and DLRP.
研究领域:
食品科学
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微生物学
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药理学
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生物技术
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PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 18
·8739-8751
·
IF 3.925
Quansheng Wu,Songhua Zhou,Chengbing Fu,Jiesong Zhang,Bo Chen,Hongyan Pan,Qian Lin
For the mono-metal Pd based catalysts, the O–O bond in O2 and H2O2 is easily dissociated due to the higher energy sites of Pd, which decreases the selectivity and productivity of H2O2. To address this issue, non-noble metals M (M = Sn, Fe, Co, Ni) were doped into palladium precursors to create a series of yolk–shell structure bimetallic catalysts Pd–M@HCS (H = hollow; C = carbon; S = sphere) with Pd–M as the core and porous carbon as the shell. The results indicated that the catalyst Pd–M@HCS doped with Sn, Fe, Co, and Ni has a weak dissociation ability for O2, and both the adsorption and dissociation ability for H2 are improved. Of these catalysts, the catalyst Pd–Sn@HCS gives the best performance, which is benefited by the suitable Pd–Sn nanoparticle size and the best Pd2+/Pd0 ratio. The influence of the nPd/nSn ratio in the Pd–Sn@HCS system on catalyst performance was further explored, and the findings revealed that Pd–Sn@HCS had the best catalytic activity and stability when the nPd/nSn ratio was 2. The catalyst's H2 conversion, H2O2 selectivity, and productivity were 35%, 97%, and 3961 mmol gPd−1 h−1, respectively. After 10 cycles, the H2O2 productivity of this catalyst remained at 92.1%, which was greater than the H2O2 productivity of the typical Pd-based catalyst Pd/C, which stayed at 37% under the same conditions.
研究领域:
催化
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化学工程
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材料科学
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无机化学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Yue Wang,Yaning Li,Meiwen Cao,Zeyu Yan,Fenggang Wei,Tianhao Ma,Ruochen Xie,Hongchao Ma
Tumor hypoxia, limited H 2 O 2 levels, and abundant glutathione (GSH) in the tumor microenvironment (TME) pose challenges for high-efficiency reactive oxygen species (ROS)-mediated therapies. To address these limitations, an intelligent ROS-mediated nanoplatform, Ce6@ZIF-8/PDA/CuO 2 /HA (CZPCH), with photodynamic activity, self-supplied H 2 O 2 , Fenton-like reaction, GSH-depletion and photothermal activity was designed for efficient tumor treatment. In the acidic TME, CuO 2 in CZPCH deposits into Cu 2+ and H 2 O 2 , consuming GSH and triggering Fenton-like reactions to generate hydroxyl radical (•OH), enabling efficient chemodynamic therapy (CDT). Moreover, the presence of Ce6 and PDA enables CZPCH to exhibit excellent singlet oxygen ( 1 O 2 ) and temperature increase performance 660 nm and 808 nm laser irradiation, respectively, resulting in efficient photodynamic therapy (PDT) and photothermal therapy (PTT) efficacy. Interestingly, the temperature increase from PTT significantly enhances •OH and 1 O 2 generation, achieving high-efficiency ROS-based therapeutic efficacy. This synergistic CDT/PDT/PTT treatment demonstrates excellent anti-tumor efficacy and illuminates novel ROS-based therapeutic strategy design.
研究领域:
纳米技术
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癌症治疗
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生物医学工程
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材料科学
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产品关联
DOI
高影响因子
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