INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2023
·Vol. 24
·期 8
·7354
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IF 5.6
Ning Han,Jie Li,Feifei Zhao,Yangyang Li,Jun Wang,Xiaolan Dai,Dongping Zeng,Wenguang Xiong,Zhenling Zeng
Streptococcus suis, an encapsulated zoonotic pathogen, has been reported to cause a variety of infectious diseases, such as meningitis and streptococcal-toxic-shock-like syndrome. Increasing antimicrobial resistance has triggered the need for new treatments. In the present study, we found that isopropoxy benzene guanidine (IBG) significantly attenuated the effects caused byS. suisinfection, in vivo and in vitro, by killingS. suisand reducingS. suispathogenicity. Further studies showed that IBG disrupted the integrity ofS. suiscell membranes and increased the permeability ofS. suiscell membranes, leading to an imbalance in proton motive force and the accumulation of intracellular ATP. Meanwhile, IBG antagonized the hemolysis activity of suilysin and decreased the expression ofSlygene. In vivo, IBG improved the viability ofS. suisSS3-infected mice by reducing tissue bacterial load. In conclusion, IBG is a promising compound for the treatment ofS. suisinfections, given its antibacterial and anti-hemolysis activity.Keywords:Streptococcus suis;isopropoxy benzene guanidine;antibacterial;anti-hemolysis;suilysin
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FASEB JOURNAL
·
2023
·Vol. 37
·期 4
·e22866
·
IF 4.8
Shan-Yan Gao,Yong-Ping Liu,Ri Wen,Xin-Mei Huang,Ping Li,Yu-Hang Yang,Ni Yang,Tie-Ning Zhang
Uncontrolled diabetes causes a catabolic state with multi-organic complications, of which impairment on skeletal muscle contributes to the damaged mobility. Kcnma1 gene encodes the pore-forming α-subunit of Ca 2+ - and voltage-gated K + channels of large conductance (BK channels), and loss-of-function mutations in Kcnma1 are in regards to impaired myogenesis. Herein, we observed a time-course reduction of Kcnma1 expression in the tibialis anterior muscles of leptin receptor-deficient (db/db) diabetic mice. To investigate the role of Kcnma1 in diabetic muscle atrophy, muscle-specific knockdown of Kcnma1 was achieved by mice receiving intravenous injection of adeno-associated virus-9 (AAV9)-encoding shRNA against Kcnma1 under the muscle creatine kinase (MCK) promoter. Impairment on muscle mass and myogenesis were observed in m/m mice with AAV9-sh Kcnma1 intervention, while this impairment was more obvious in diabetic db/db mice. Simultaneously, damaged mitochondrial dynamics and biogenesis showed much severer in db/db mice with AAV9-sh Kcnma1 intervention. RNA sequencing revealed the large transcriptomic changes resulted by Kcnma1 knockdown, and changes in mitochondrial homeostasis-related genes were validated. Besides, the artificial alteration of Kcnma1 in mouse C2C12 myoblasts was achieved with an adenovirus vector. Consistent results were demonstrated by Kcnma1 knockdown in palmitate-treated cells, whereas opposite results were exhibited by Kcnma1 overexpression. Collectively, we document Kcnma1 as a potential keeper of mitochondrial homeostasis, and the loss of Kcnma1 is a critical event in priming skeletal muscle loss in diabetes.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2023
·
IF 4.2
Junxin Chen,Caijin Zhou,Bingqi Xie,Jisong Zhang
Counter-current operation has been widely applied to enhance the extraction efficiency for systems with low distribution coefficients, which was hindered in microextraction because viscous force and surface tension dominate over gravity and inertial force. In this study, a novel design of a tube-in-tube microextractor relying on a poly tetra fluoroethylene membrane to construct a stable interface was proposed to conduct continuous counter-current extraction. Extraction kinetics for two systems with different distribution coefficients, membranes with different pore radii, and three flow rate ratios were measured as a function of residence time. For the system that toluene was used to extract 0.06 wt % phenol in water, 4.1 stages of extraction efficiency were achieved at a residence time of 9.8 min. A challenging case study (50 wt % acetone–water–toluene) was subsequently conducted to prove the high applicability for emulsion systems of this special microextractor. Lastly, a mathematical model was established and the predicted results of three flow rate ratios are in good agreement with experimental results.
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JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Min Zhu,Xi Feng,Gaoyang Qiu,Jiayin Feng,Lujun Zhang,Phillip C. Brookes,Jianming Xu,Yan He
Methanogenesis is commonly mass-produced under anaerobic conditions and serves as a major terminal electron accepting process driving the degradation of organic biomass. In this study, a cofactor of methanogenesis (coenzyme M, CoM) and a classic methanogensis inhibitor (2-bromoethanesulfonate, BES) were added at different concentrations to investigate how methanogenesis would affect PCP degradation in flooded soil. Strikingly, the processes of methanogenesis and PCP degradation were simultaneously promoted with CoM , or inhibited with BES, significantly ( p < 0.05). High-throughput sequencing for soil bacterial and archaeal community structures revealed that members of Desulfitobacterium , Dethiobacter , Sedimentibacter, Bacillus and Methanosarcina might act as the core functional groups jointly perform PCP degradation in flooded soil, possibly through assisting microbial mediated dechlorination in direct organohalide-respiration, and/or indirect co-metabolization in complex anaerobic soil conditions. This study implied an underlying synergistic coupling between methanogenesis and dechlorination, and provided insights into a novel consideration with respect to coordinating methanogenesis while promoting anaerobic degradation of PCP for complex polluted soil environment, which is necessary for the improved all-win remediation.
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JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
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2018
·Vol. 94
·期 5
·1474-1479
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IF 2.587
Yuqiang Zhang,Jun Li,Yang Jin,Ming Chen,Defang Yu
BACKGROUND Over the past few decades, tri-n-butyl phosphate (TBP) has been a widely used and irreplaceable extractant that can extract a variety of heavy metal ions and inorganic acids. The conventional process of tri-n-butyl phosphate synthesis has the problem of low yield. The in situ vapor stripping method was developed to intensify the tri-n-butyl phosphate synthesis reaction. RESULTS In this work, the coupling process of in situ vapor stripping and reaction was proposed for the first time in the process of synthesis of TBP. It was found that the reaction of high concentration hydrogen chloride and 1-butanol would produce water, which resulted in increasing the possibility of side reactions, especially when the temperature was higher than 55 °C. In the lower temperature range, n-pentane was selected as the stripping medium, which effectively removed hydrogen chloride from the reaction system by in situ vapor stripping. The FT-IR analysis confirmed that TBP can be synthesized successfully by the vapor stripping process. In addition, the effects of n-pentane volume, temperature and stripping time on the yield of TBP and the amount of hydrogen chloride stripped were investigated. By applying the optimum reaction conditions, the yield of TBP was increased to 94%, larger than that of the current industrial process whose yield is only 80%. CONCLUSION The in situ vapor stripping process is demonstrated to be an effective method for intensification of the esterification process in TBP synthesis. It can be used for other reactions which generate volatile gases as the byproduct. © 2018 Society of Chemical Industry
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ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Yi-Tao Zou,Zhou Fang,Yuan Li,Runmei Wang,Hao Zhang,Kevin C. Jones,Xin-Yi Cui,Xin-Yao Shi,Daixia Yin,Chao Li,Zhao-Dong Liu,Lena Q. Ma,Jun Luo
Widespread use of organophosphorus flame retardants (OPFRs) and their ubiquity in water results in the need for a robust and reliable monitoring technique to better understand their fate and environmental impact. In situ passive sampling using the diffusive gradients in thin-films (DGT) technique provides time-integrated data and is developed for measuring OPFRs here. Ultrasonic extraction of binding gels in methanol provided reliable recoveries for all tested OPFRs. Diffusion coefficients of TCEP, TCPP, TDCPP, TPrP, TBP, and TBEP in the agarose diffusive gel (25 °C) were obtained. The capacity of an HLB binding gel for OPFRs was >115 μg per disc, and the binding performance did not deteriorate with time up to 131 days. DGT performance is independent of typical environmental ranges of pH (3.12–9.71), ionic strength (0.1–500 mmol L–1), and dissolved organic matter (0–20 mg L–1), and also of diffusive layer thickness (0.64–2.14 mm) and deployment time (3–168 h). Negligible competition effects between OPFRs was found. DGT-measured concentrations of OPFRs in a wastewater treatment plant (WWTP) effluent (12–16 days) were comparable to those obtained by grab sampling, further verifying DGT’s reliability for measuring OPFRs in waters.
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GREEN CHEMISTRY
·
2017
·Vol. 20
·期 1
·188-196
·
IF 9.125
Lin Tao,Mingmei Zhong,Jian Chen,Sanjeevi Jayakumar,Lina Liu,He Li,Qihua Yang
An efficient heterogeneous catalytic system for hydroformylation of long-chain alkenes is highly desirable for both academy and industry. In this study, an IL-in-oil Pickering emulsion system was employed for heterogeneous hydroformylation of 1-dodecene with Rh-sulfoxantphos as the catalyst and surface modified dendritic mesoporous silica nanospheres (DMSN) as the stabilizer. The IL-in-oil Pickering emulsion system outperformed IL-oil biphase, water-in-oil Pickering emulsion and IL-oil micelle system under similar reaction conditions to afford n/b ratio of 98 : 2, chemoselectivity of 94% and TOF of 413 h−1, among the highest ever reported for IL-oil biphase hydroformylation of long-chain alkenes. The high efficiency of IL-in-oil Pickering emulsion was primarily attributed to the increased interface area and unique properties of ILs. Studies also revealed that solid stabilizers with large and open pore channels could greatly increase the reaction rate of Pickering emulsion systems by accelerating the diffusion rate. The recyclable IL-in-oil Pickering emulsion is promising not only for hydroformylation of long-chain alkenes but also for catalytic reactions with immiscible liquids.
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Energy & Environmental Materials
·
2022
·
IF 13.443
Chundong Liu,Fengcai Lei,Maogang Gong,Xiaoming Zhou,Xiaofei Zhao,Zhen Li,Chao Zhang,Baoyuan Man,Jing Yu
In situ surface-enhanced Raman scattering (SERS) is a widely used operando analytical technique, while facing numerous complex factors in applications under aqueous environment, such as low detection sensitivity, poor anti-interference capability, etc., resulting in unreliable detectability. To address these issues, herein a new hydrophobic SERS strategy has been attempted. By comprehensively designing and researching a SERS-active structure of superhydrophobic ZnO/Ag nanowires, we demonstrate that hydrophobicity can not only draw analytes from water onto substrate, but also adjust “hottest spot” from the bottom of the nanowires to the top. As a result, the structure can simultaneously concentrate the dispersed molecules in water and the enhanced electric field in structure into a same zone, while perfecting its own anti-interference ability. The underwater in situ analytical enhancement factor of this platform is as high as 1.67 × 10 11 , and the operando limited of detection for metronidazole (MNZ) reaches to 10 −9 M. Most importantly, we also successfully generalized this structure to various real in situ detection scenarios, including on-site detection of MNZ in corrosive urine, real-time warning of wrong dose of MNZ during intravenous therapy, in situ monitoring of MNZ in flowing wastewater with particulate interference, etc., demonstrating the great application potential of this hydrophobic platform. This work realizes a synergistic promotion for in situ SERS performance under aqueous environment, and also provides a novel view for improving other in situ analytical techniques.
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CHEMICAL RESEARCH IN TOXICOLOGY
·
2023
·
IF 4.1
Aixuan Wang,Qiang Zhao,Minglu Liu,Yang Wang,Guode Zhao,Weiwei Li,Ying Peng,Jiang Zheng
Tolterodine (TOL) is an antimuscarinic drug used for the treatment of patients with overactive bladder presenting urinary frequency, urgency, and urge incontinence. During the clinical use of TOL, adverse events such as liver injury took place. The present study aimed at the investigation of the metabolic activation of TOL possibly associated with its hepatotoxicity. One GSH conjugate, two NAC conjugates, and two cysteine conjugates were found in both mouse and human liver microsomal incubations supplemented with TOL, GSH/NAC/cysteine, and NADPH. The detected conjugates suggest the production of a quinone methide intermediate. The same GSH conjugate was also observed in mouse primary hepatocytes and in the bile of rats receiving TOL. One of the urinary NAC conjugates was observed in rats administered TOL. One of the cysteine conjugates was found in a digestion mixture containing hepatic proteins from animals administered TOL. The observed protein modification was dose-dependent. CYP3A primarily catalyzes the metabolic activation of TOL. Ketoconazole (KTC) pretreatment reduced the generation of the GSH conjugate in mouse liver and cultured primary hepatocytes after TOL treatment. In addition, KTC reduced the susceptibility of primary hepatocytes to TOL cytotoxicity. The quinone methide metabolite may be involved in TOL-induced hepatotoxicity and cytotoxicity.
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JOURNAL OF POWER SOURCES
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2022
·
IF 9.794
Haoru Wu,Likun Zhu,Yongzhu Fu
Molecular organosulfides as a class of cathode materials have the advantages of high capacity and sustainability. However, they could dissolve in organic liquid electrolytes resulting in capacity decay upon cycling. Herein, we report a strategy to anchor 1,2-benzenedithiolate (1,2-BDT) molecule as an example from dissolution by forming organosulfide-metal complex in situ in the cathode of a lithium battery . Copper nanowires (Cu NWs) are synthesized hydrothermally and then interweaved with carbon nanotubes as the current collector. Lithium 1,2-benzenedithiolate (1,2-LBDT) catholyte is added in the current collector and evaluated in a lithium half cell. In the first charge, 1,2-LBDT is converted to 1,2-BDT radicals which react with Cu NWs forming copper benzene-1,2-dithiolato complex Cu[Cu-(BDT) 2 ]. Cu NWs are consumed and the formed Cu[Cu-(BDT) 2 ] is hardly dissolved in the electrolyte. The whole process is revealed by Raman, X-ray diffraction, and quadrupole time-of-flight mass spectrometry and the redox mechanism is proposed. The cell exhibits a sloping discharge voltage profile with four visible plateaus, initial discharge capacity of 211.8 mAh g −1 , and 1000 cycles at 0.5 C rate.
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EBioMedicine
·
2022
·
IF 11.205
Xiaoqi Hong,Yujin Jian,Shenghao Ding,Jianpo Zhou,Xiaoli Zheng,Huimin Zhang,Butian Zhou,Canbin Zhuang,Jieqing Wan,Xiaoping Tong
Summary Background Stroke is one of the most common neurological diseases in the world and is clinically manifested by transient or permanent brain dysfunction. It has a high mortality and disability rate, which severely affects people's health and diminishes the quality of life. However, there is no efficient treatment that can be considered curative and there are other less well-known theories of pathogenesis. Therefore, it is imperative to gain a full understanding of the pathophysiology of ischemia and to seek new therapeutic strategies. Methods We first examined Kir4.1 channel and myelin based protein (MBP) expression in brain tissues from acute ischemic patients by Western blotting. We then established a transient ischemic mouse model (tMCAO) to conduct molecular, cell biological, transmission electron microscopy and pharmacokinetic studies, as well as in Kir4.1 cKO mice. Finally, neuroimaging and behavioral analyses were used to examine whether activation of Kir4.1 channel by luteolin could contribute to neuronal functional recovery in ischemic stroke. Findings In acute ischemic stroke patients, we first demonstrated that Kir4.1 ion channels were greatly impaired and a severe demyelination of axons occurred in ischemic infarction area of cerebral cortex in these patients. Further evidence showed that the deficits of Kir4.1 channels in NG2 glia led to the myelin loss of axons in a transient ischemic mouse model (tMCAO). Treating ischemic mice with a natural botanical extract, luteolin augmented Kir4.1 channel currents in NG2 glia and consequently promoted remyelination of axons, alleviated the infarction area and ultimately improved motor function in a series of behavioral tests. Interpretation Targeting Kir4.1 ion channels expressed in NG2 glial cells by luteolin treatment highlights an effective therapeutic strategy for a prompt brain functional recovery in ischemic stroke. Funding This work was supported by grants from the Ministry of Science and Technology China Brain Initiative (2022ZD0204702, to X.T.), the National Natural Science Foundation of China ( 82271466 , 82171279 , 31970904 and 31571063 ), the Program for Professor of Special Appointment (Eastern Scholar for Dr. X.T.) at Shanghai Institutions for Higher Learning ( 1510000084 ), Shanghai Pujiang Talent Award ( 15PJ1404600 ), Shanghai Municipal Science and Technology Major Project ( 2018SHZDZX05 ) and Shanghai Science and Technology Project ( 17411954000 ).
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Jing Li,Youyou Yang,Chaohua Tang,Shengnan Yue,Qingyu Zhao,Fadi Li,Junmin Zhang
Intramuscular fat (IMF) content is associated with lamb flavor and largely varies between individuals of the same breed. However, studies investigating the effects of IMF variations on lipid profiles and aroma compounds are limited. Here, we performed lipidomic and volatilomic profiling of high-IMF and low-IMF Hu lambs. Triglycerides and diglycerides in the high-IMF group were significantly higher than those in the low-IMF group ( p < 0.05). Seventy-nine of 842 lipids identified were significantly different between the groups under positive and negative ion modes (variable importance in projection > 1, p < 0.05). Volatilomic analyses revealed that the aroma profiles also differed between the groups. Fifteen aroma compounds, mainly originating from lipid oxidation, could be responsible for this difference. Thus, our findings provide a comprehensive understanding of the increases in IMF content that drive consumers’ satisfaction and also provide a basis for underpinning breeding value for IMF.
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