ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Di Cheng,Yaping Zhang,Dongxiao Wen,Zhuangzhuang Guo,Huaixia Yang,Yanju Liu,Jinming Kong
A novel, simple, and label-free amplification electrochemical impedance method for quantitative detection of Human T-lymphotropic virus types II(HTLV-II) via click chemistry-mediated of hairpin DNA probes (hairpins) with polymers was developed. The hairpins were firstly attached to the gold electrode surface by an S-Au bond, the azido terminals of hairpins were close to the electrode surface, which make it difficult to be approached. After hybridizing with HTLV-II, the hairpins were unfolded and experienced a big configuration change, which made the azido terminals of the hairpins available to conjugate with alkynyl-containing polymer, called P(DEB-DSDA), formed by 1,4-diacetylenebenzene (DEB) and 4,4′-Diazido-2,2′-stilbenedisulfonic acid disodium salt tetrahydrate (DSDA) via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). With amount of P(DEB-DSDA) conjugated with the hairpin probes via click polymerization, its electrochemical signal can have a great amplification. Under optimized experimental conditions, this new probe showed a low detection limit of 0.171 pM with a good liner in the range of 1 pM-1 nM. Meanwhile, the biosensor also exhibited good selectivity and reliability in detection of real serum samples, indicating that it has great application potential in clinical DNA diagnosis and detection.
研究领域:
生物化学
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分析化学
·
生物技术
·
病毒学
·
电化学
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DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2021
·
IF 6.025
Xingyao Pei,Haiyang Jiang,Xinyu Liu,Liuan Li,Cun Li,Xilong Xiao,Daowen Li,Shusheng Tang
T-2 toxin, a food-derived mycotoxin , has been identified as a neurotoxin . Nonetheless, T-2 toxin-induced neuroinflammation has never been revealed. As an important therapeutic target for inflammatory diseases and cancers, the role of high mobility group B1 (HMGB1) in mycotoxin-mediated neurotoxicity remains a mystery. In current study, we found that PC12 cells were sensitive to trace amounts of T-2 toxin less than 12 ng/mL, distinguished by decreased cell viability and increased release of lactate dehydrogenase (LDH). Oxidative stress and mitochondrial damage were observed in PC12 cells, manifested as accumulation of oxidative stress products, up-regulation of Nrf2/HO-1 pathway and decrease of mitochondrial membrane potential (MMP), leading to mitochondria-dependent apoptosis . Meanwhile, we first discovered that tiny amounts of T-2 toxin triggered neuroinflammation directly, including raising the expression and translocation of NF-κB and promoting secretion of proinflammatory cytokines such as TNF-α, IL-6, IL-8 and IL-1β. Most interestingly, the increased of HMGB1 was detected both inside and outside the cells. Conversely, HMGB1 siRNA reduced T-2 toxin-mediated oxidative stress, apoptosis and neuroinflammatory outbreak, accompanied by lessened caspase-3 and caspase-9, and decreased secretion of pro-inflammatory cytokines. Taken together, T-2 toxin-stimulated PC12 cells simultaneously displayed apoptosis and inflammation, whereas HMGB1 played a critical role in these neurotoxic processes.
研究领域:
毒理学
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神经科学
·
细胞生物学
·
药理学
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DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2021
·Vol. 29
·期 1
·1-11
·
IF 3.097
Li Kaiqi,Chen Long,Xiong Zuochun,Xiong Chengdong,Chen Dongliang
Amino-functionalized six-membered cyclic carbonate tert-butyl(2-oxo-1,3-dioxan-5-yl) carbamate (TBC) was synthesized with tert-Butyloxycarbonyl (Boc) as protected group. Due to its thermal properties, ring-opening polymerization (ROP) carried out in a relatively low temperature, and Sn (Oct) 2 as catalyst. A series of copolymers were prepared with L-lactide (LA), ε-Caprolactone (CL) as co-monomers, searching for proper components and ratios. The protecting Boc-group was easy to remove by using trifluoroacetic acid (TFA). The structures of TBC and copolymers were confirmed by 1 H NMR and 13 C NMR analysis. Copolymers were characterized by DSC, GPC and water contact angle analysis. The introduction of pendant amino groups resulted in a significant enhancement of hydrophilicity of the copolymers.
研究领域:
材料科学
·
高分子化学
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DOI
高影响因子
RSC Advances
·
2014
·Vol. 5
·期 3
·1969-1978
·
IF 3.708
Xiaoxiao Chu,Pengyao Xing,Shangyang Li,Mingfang Ma,Jingcheng Hao,Aiyou Hao
As a small molecule, N-(9-fluorenylmethoxy carbonyl)-glycine (FG) possesses a hydrophobic plane domain (π-conjugated section) and a hydrophilic domain (amino acid section), which can be designed into multi-dimensional self-assembly structures under dual tuning. Through controlling the concentration of T-shaped FG, the transformation between various morphologies has been achieved: vesicles are obtained at low concentrations (0.0025–0.005 wt%); helical fibers can be found at the concentration of 0.2 wt% though Gly has no stereocenter, resulting in the birth of chiral organization; fibrous bundles can accumulate into a three dimensional network to finally form a supramolecular gel. Taking gel as an example, we devised a variety of nanostructures including nanoparticles, microparticles, nanoribbons and membranes obtained by adding base. The mechanism of self-assembly formation has been investigated and this system is hoped to enrich the category of nanomaterials from amino acid or short peptides.
研究领域:
纳米材料
·
自组装化学
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超分子化学
·
生物材料
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分子自组织
·
软物质物理
·
肽基功能材料
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DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2022
·
IF 6.371
Wei Shen,Liang Qiao,Juan Ding,Yongming Sui
Tailoring tantalum disulfide (TaS 2 ) electrocatalysts with tunable stacking architecture and phase structure is of great importance for advancement of hydrogen evolution reaction (HER). In this work, stacking architecture- and phase- modulated tantalum disulfide nanosheets are reported through a facile liquid phase synthesis strategy. Accordion-like nanosheets , vertical nanosheets and ultrathin nanosheets are named according to their obvious stacking architecture, and 1 T-2 H mixed phase structures containing different degrees of 2 H phases are realized. High-resolution transmission electron microscopy shows that there are obvious lattice defects in the nanosheets. Tantalum disulfide nanosheets synergistically integrate multiple advantages of nanostructure engineering, phase engineering and defect engineering. In the HER process, the ultrathin tantalum disulfide nanosheet structure is conducive to the in-plane electron transfer , and its close contact with the electrode surface makes the charge transfer faster and has the best HER activity. The overpotential of the ultrathin nanosheet electrocatalyst at 10 mA cm −2 is 280 mV. After 10,000 CV cycles and over 40 h of i-t test, the catalyst still has high stability in acidic media. Theoretical calculations show that the addition of 2 H phase structure and rich defects improves the intrinsic activity and active sites, and jointly promotes the improvement of HER performance. Our work integrates a variety of optimal regulation methods into TaS 2 nanosheets, providing a new idea for the construction of advanced electrocatalysts for water electrolysis.
研究领域:
材料科学
·
电催化
·
纳米材料
·
能量转换
·
计算化学
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DOI
高影响因子
Food Bioscience
·
2026
·
IF 5.9
Kai Cheng,Gang Wang,Jianhua Xie,Zhibing Huang
Penicillium polysaccharides exhibit immunomodulatory activity, different polysaccharides produced by Penicillium EF-2 during fermentation were altered by adding different concentrations of genistein as an exogenous additive to change the fermentation environment. Structural characterization of the polysaccharides was performed and their activities were evaluated, and it was found that the Penicillium polysaccharides became more porous and their molecular weight decreased after the addition of genistein, thereby increasing their biological activity. Combined cell-based and animal studies demonstrated that genistein addition enhances the bioactivity of extracellular polysaccharides produced by Penicillium fermentation. However, for polysaccharide yield in Penicillium fermentation, polysaccharide content decreased at 3 d in both 0.5 g/L genistein (G_0.5E) and 1.5 g/L genistein (G_1.5E) treatments. Furthermore, it was concluded that polysaccharides may influence T cell differentiation by regulating energy metabolism, thereby exerting an immunosuppressive regulatory effect.
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高影响因子
Science Immunology
·
2026
·Vol. 11
·期 121
·
IF 16.4
Weiwei Yu,Ruya Sun,Xiaoyang Lu,Qiqi Deng,Heng Zhang,Sheng Chen,Siqi Dai,Tianyi Hu,Xiangyuan Li,Yidong Yang,Lihao Chen,Zhexu Chi,Jian Zhang,Siwen Zhang,Yiyi Zhu,Dehang Yang,Qianzhou Yu,Zhen Wang,Quanquan Wang,Fan Yang,Qian Xiao,Kefeng Ding,Di Wang
Tumor cells promote metabolic dysregulation of immune cells by controlling the metabolic landscape of the tumor microenvironment. It is unclear whether tumors restrict specific nutrients to drive rapid growth and immune evasion in addition to the overconsumption of nutrients to support anabolism. We identified that up-regulation of solute carrier family 7 member 1 (SLC7A1) increased arginine utilization and promoted tumor growth, whereas down-regulation of SLC7A2 decreased lysine catabolism to support immune evasion. Repression of lysine catabolism in tumor cells reduced glutaconic acid (GC), a medium-chain acyl-CoA dehydrogenase–dependent lysine catabolite that has immunostimulatory effects on antitumor CD8 T cells. GC modified pyruvate kinase M2 (PKM2) through posttranslational glutaconylation at key lysine residues Lys 336 (K336) and K337. This modification reinforced PKM2 dimers, transcriptionally driving metabolic reprogramming and reinvigorating antitumor CD8 T cells. Our study highlights an amino acid trade-off that dynamically optimizes the metabolic preferences of tumors to promote proliferation and immune evasion.
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产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2026
·
IF 5.7
Ying Huang,Jiale Li,Yingzhou Qin,Bo Qie,Qianqian Wang,Cuijuan Liu,Yimin Zhu
A cyclic peptide binding with TIM-3, TBP1, was screened by a novel CX 12 C cyclic peptide display library base on bacterial surface display. • TBP1 effectively disrupted TIM-3 interactions with CEACAM-1 and PtdSer. • TBP1 restored the function of exhausted T cells in vitro . • TBP1 synergized with anti-PD-1 antibody, improving anti-tumor immunity in vivo . Immune checkpoint blockade (ICB) therapies, particularly antibodies blocking, the programmed cell death protein 1 (PD-1)/programmed cell death-ligand 1 (PD-L1), have shown clinical success but remain limited by suboptimal response rates, often due to insufficient immune activation. T-cell immunoglobulin and mucin domain-containing protein-3 (TIM-3), a non-redundant immune checkpoint frequently co-expressed with PD-1, drives severe T-cell exhaustion in solid tumors and contributes to resistance against anti-PD-1/PD-L1 therapy. Thus, dual blockade of TIM-3 and PD-1 may restore more effective anti-tumor immunity. Compared to antibodies, low-molecular-weight peptides offer advantages in combination therapy, including better tissue penetration, lower immunogenicity, improved tolerability and low production costs. Cyclic peptides, in particular, provide enhanced biological activity and proteolytic stability. Here, we present TBP1, a cyclic peptide identified via a bacterial surface display library, which binds TIM-3 with high affinity and specificity. Competitive blocking assays and molecular docking confirmed that TBP1 effectively disrupts TIM-3 interactions with Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1) and Phosphatidylserine (PtdSer). In vitro , TBP1 restored exhausted T-cell function by disrupting the TIM-3/CEACAM-1 interaction. In CT26 tumor-bearing mice, TBP1 synergized with an anti-PD-1 antibody, enhancing anti-tumor immunity by promoting CD4 + T cell infiltration and IL-2 secretion. These findings identify TBP1 as a novel TIM-3-targeting cyclic peptide and support its potential application in combination immune checkpoint blockade. Download: Download high-res image (372KB) Download: Download full-size image
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高影响因子
Science Immunology
·
2025
·Vol. 10
·期 108
·
IF 16.3
Limin Cao,Li Wang,Zhihong Li,Xia Wei,Jinqiu Ding,Chun Zhou,Xia Chen,Zhicheng Huang,Zhugui Shao,Junchen Shen,Hongfei Lou,Keqing Zhao,Yuwei Huang,Yuanqin Yang,Han Liu,Yumeng Sun,Junling Niu,Shan Jiang,Rong Lu,Longhai Tang,Xiaoming Zhang,Haibing Zhang,Yichuan Xiao,Jianfeng Chen,Shixin Ma,Chengjiang Gao,Guangxun Meng,Li Liu,Zhaozhu Qiu,Haopeng Wang,Liufu Deng,Youqiong Ye,Xin-Ming Jia,Huabin Li,Hui Xiao
The volume-regulated anion channels (VRACs) transport osmolytes, neurotransmitters, and cyclic GMP-AMP (cGAMP) across the cell membrane to regulate cell volume and host defense. We report that the leucine-rich repeat–containing 8A/C (LRRC8A/C) VRAC plays a crucial role in immune responses to radiotherapy and chemotherapy for cancer. VRACs transfer cGAMP from irradiated cancer cells to infiltrating CD4 and CD8 T cells, thus enhancing their effector functions. TCR signaling acts as a physiological signal to open the VRAC pore through phosphatidylinositol 4,5-bisphosphate [PI(4,5)P 2 ] and reactive oxygen species (ROS). This allows the rapid uptake of cGAMP and STING activation in mouse and human T cells and induction of interferon-α/β, which up-regulate granzymes and IFN-γ in CD8 T cells. Inhibition of the extracellular hydroxylases CD39 and ENPP1 maintains extracellular ATP and cGAMP, which promotes VRAC-enhanced CD8 T cell anticancer function. Thus, the transfer of cGAMP to T cells by VRACs may be a strategy that can be targeted in future cancer therapies.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2025
·
IF 6
Hengyang Liu,Wanyuan Li,Yong Xu,Enzhu Hu,Guangchao Qin,Kunhong Hu
A 1 T/2H-MoS 2 (THM) lubricant additive was synthesized via a solvothermal method to improve the lubrication of poly-alpha-olefin (PAO) and reduce the negative influence of diesel carbon soot (CS). The THM was then modified with Cu to further enhance the wear resistance. The average interlayer spacing of MoS 2 in THM and the Cu-modified THM (CTHM) was 0.99 nm, significantly larger than the 0.63 nm of 2H-MoS 2 nanoparticles. In PAO, CTHM demonstrated a 45 % improvement in friction reduction and a 77 % increase in wear resistance compared to 2H-MoS 2 . While the friction reduction of CTHM was similar to that of THM, CTHM exhibited 36 % superior wear resistance. In CS-contaminated PAO, CTHM outperformed 2H-MoS 2 with a 37 % reduction in friction and a 29 % enhancement in wear resistance. The Cu modification reduced the oxidation of CTHM and the conversion of 1 T-MoS 2 to 2H-MoS 2 in the tribofilm, contributing to improved wear resistance. The CS contamination negatively influenced the lubrication by accelerating MoS 2 oxidation, catalyzing the transition from 1 T-MoS 2 to 2H-MoS 2 , and inhibiting Cu incorporation into the tribofilm. Nonetheless, the effect of CS on CTHM and THM was less severe than on 2H-MoS 2 . This work provides an alternative method to alleviated the antagonism between MoS 2 and CS.
研究领域:
材料科学
·
润滑工程
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DOI
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2025
·
IF 11.5
Nan Li,Weiwei Mu,Zhenxing Xia,Qingping Ma,Ruihua Feng,Panpan Gu,Qinglin Yang,Shuying Gao,Weihan Zhang,Suyun Wei,Yi Zheng,Wei Zhao,Yongjun Liu,Na Zhang
Therapeutic tumor vaccines, which activate self-T cells to eliminate tumors, hold tremendous promise in future cancer immunotherapy with high specificity and low side effects. To effectively activate T cell, dendritic cells (DCs) need simultaneously provide three indispensable signals to naive T cells, including MHC-antigen signal, costimulation signal and cytokine stimulation. However, current marketed therapeutic tumor vaccines still suffer from lacking the ability to activate three stimulation signals at the same time, which resulted to the low response rate and unsatisfied therapeutic efficiency. Here, we proposed a soluble microneedle-based tumor vaccines (TR-12@LGMN) which facilitate triple activation of antigen presentation and induce high immune response rate. First, the melanoma-specific antigen tyrosinaserelated protein-2 (Trp2), Resiquimod (R848), and IL-12 mRNA co-loaded liposomes (TR-12@LIPO) was prepared. The TR-12@LIPO ensured triple-activating three essential signals of antigen presentation through enhancing the binding of MHC I-antigen peptides to T cell receptor (TCR), the binding of CD80/86 on DCs to CD28 on T cells, and the release of cytokines for T cells activation. Second, TR-12@LIPO was co -dispersed in polyvinylpyrrolidone-polyvinyl alcohol (PVP-PVA) matrix with granulocyte-macrophage colony stimulating factor (GM-CSF) to prepare TR-12@LGMN by mold method. The TR-12@LGMN was quadrilateral shape with sufficient mechanical strength for skin piercing. After skin insertion, TR-12@LGMN dissolved in the skin interstitial fluid to release GM-CSF and TR-12@LIPO. DCs were recruited by GM-CSF and uptaked TR-12@LIPO. TR-12@LIPO showed enhancement of cross-presentation by antigen cytoplasmic delivery, DCs maturation and IL-12 secretion. In vivo results showed that TR-12@LGMN could efficiently activate CD8 + T cells, induce antigen-specific cytotoxic T cells (CTLs) and memory T cell (T m ). Ultimately, strong anti-tumor immunity and long-term durable tumor control were achieved in the B16F10 tumor prevention and treatment model. Overall, our work proposes a triple-activated antigen presentation strategy and designs a microneedle-based tumor vaccine for skin delivery, revealing a potential promising direction for the development of new therapeutic tumor vaccines methods.
研究领域:
肿瘤免疫学
·
生物医学工程
·
药剂学
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DOI
PubMed
高影响因子
ChemNanoMat
·
2025
·Vol. 11
·期 7
·e202500102
·
IF 2.6
Yuanchao Li,Shixing Han,Pengchao Liang,Xiaokun Shang,Qi Li,Jingjing Ma,Songlin Wang,Li Yang,Guangri Xu
The Na x MnO 2 ( x < 0.44) material with a T-type tunnel phase shows excellent cycling performance, but low initial specific capacity. The Na x MnO 2 ( x > 0.6) with P2-type phase exhibits high initial specific capacity, yet poor cycling performance. Layer-tunnel hybrid materials has served as cathode materials for sodium-ion batteries (SIBs) due to the integration of high specific capacity and superior cycling performance. Herein, sodium manganite composites is synthesized with tunnel phase containing a small amount of P2-type layered phase in Na 0.44 MnO 2 materials using citric acid as the dispersant. The citric acid can promote the transition of the T-P2 phase and contribute to the uniform distribution of elements. The as-prepared Na 0.44 MnO 2 with layered-tunnel hybrid phase exhibits the best overall electrochemical performances with a capacity retention of 68% after 300 cycles at 1 C and an initial discharge capacity of 143.7 mA h g −1 , due to combination of the advantages of layered and tunnel structure. This work provides a new strategy for synthesizing transition metal oxides with hybrid phase as the cathode of advanced SIBs.
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高影响因子
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