APPLIED SURFACE SCIENCE
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2026
·
IF 6.6
Hao Liang,Min Li,Zengdong Zhang,Meixuan Ouyang,Hebing Pei,Ruibin Guo,Nijuan Liu,Zunli Mo
Chiral carbon dots synthesized via low-temperature one-step hydrothermal method. • Constructing an “on–off-on” fluorescence sensing mode for dual detection. • Demonstrating exceptional detection sensitivity for Hg 2 ⁺ and GSH. • Verified the recognition mechanism potentially mediated by chiral fluorescent groups. Carbon dots (CDs) are widely used in fields such as ion recognition and small molecule detection due to their unique fluorescent properties. In this study, L-CD, a “on–off-on” fluorescent sensor for highly selective detection of Hg 2+ and glutathione (GSH), was rapidly synthesized via a low-temperature one-step hydrothermal reaction. The results revealed that chiral functional groups on its surface may act as luminescent moieties interacting with Hg 2+ and GSH, leading to fluorescence switching. Hg 2+ exhibited a unique two-phase response, while GSH can rapidly and stably restore the fluorescence of the L-CD + Hg 2+ . Mechanistic analysis suggests that the process involves not only static quench, but also may include dynamic quenching. Moreover, L-CD can be used to chemically modify filter paper to enable visual semi-quantitative detection of Hg 2+ and GSH. In actual samples, L-CD exhibit excellent accuracy (RSD < 5.0%) and can serve as reliable fluorescent sensors for the detection of Hg 2 ⁺ in complex system and the efficient identification of GSH in biological samples. Conclusively, this work synthesized chiral CDs with high quantum yields at mild conditions and validated the potential mechanism involving chiral fluorescent groups, providing essential reference for in-depth investigation into the luminescent behavior of CDs. Download: Download high-res image (122KB) Download: Download full-size image
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TALANTA
·
2026
·
IF 6.7
Xuedong Jia,Hongyuan Guo,Xiu Zhao,Zhuo Shi,Yingchun Zhu,Suozhu Wu
Monitoring nitrite in water and food is critical for ensuring human health. Herein, an electrochemical sensor was constructed for determining nitrite based on iodide probe and gold nanoparticles-poly(L-arginine)-poly(L-lysine) (AuNPs-PArg-PLys) modified electrode. The AuNPs-PArg-PLys nanocomposites were fabricated by one-step in-situ electrochemical synthesis method. Electrochemical detection of nitrite was carried out using square-wave voltammetry. With the assistance of iodide probe, the minimum detectable nitrite concentration reduced remarkably by five orders of magnitude when compared to that without this probe. The iodide-assisted AuNPs-PArg-PLys-based electrochemical sensor could determine nitrite from 1.0 nM to 10.0 μM with a detection limit of 271.8 pM. This sensor possessed the advantages of simplicity, rapidness, low detection limit, wide response range, excellent reproducibility, and high selectivity towards nitrite, which was successfully utilized to measure nanomolar-scale nitrite in tap water and beef. The proposed sensor offered potential applications in environmental protection and food safety.
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JOURNAL OF DAIRY SCIENCE
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2020
·
IF 3.333
Qianying Li,Guoyang Xie,Yutong Wang,Zoraida P. Aguilar,Hengyi Xu
ABSTRACT In this study, a new vancomycin (Van)-modified poly- l -lysine (PLL) magnetic bead (MB) technique was developed for isolation of gram-positive bacteria. The method combines magnetic separation with a multiplex PCR (mPCR) assay and gel electrophoresis for easy and rapid detection of Bacillus cereus . Vancomycin was used as a molecular ligand between the MB and the d -alanyl- d -alanine moieties on the cell wall surface of B. cereus . The PLL served as a flexible molecular tether between the MB and Van that reduced steric hindrance maintaining the biological activity of Van. The MB-PLL-Van capture nanoprobes exhibited excellent capture and isolation efficiency for B. cereus in spiked milk matrix samples without interference from the complex food matrix. The subsequent mPCR assay showed high specificity for the 4 target genes in B. cereus , the entFM , cesB , cer , and 16S rRNA genes, that were used to achieve efficient genotyping and detection. Under optimum conditions, the limit of detection reached 10 3 cfu/mL, with a dynamic range of detection at 10 3 to 10 7 cfu/mL in pure culture. Application of the MB-PLL-Van mediated mPCR assay for B. cereus in milk matrix samples achieved results similar to those of the pure culture. In addition, with a 6-h pre-enrichment of B. cereus that was spiked in milk matrix samples, the limit of detection reached 10 1 cfu/mL. The MB-PLL-Van mediated mPCR assay developed in this study could be used as a universal technology platform for the efficient enrichment and genotyping of gram-positive bacteria.
研究领域:
微生物学
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食品科学
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分子生物学
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生物技术
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高影响因子
Frontiers in Immunology
·
2022
·
IF 8.786
Wei Shujin,Jiao Dian,Xing Wanli
Both Gram-negative and Gram-positive bacteria can release vesicle-like structures referred to as bacterial extracellular vesicles (BEVs), which contain various bioactive compounds. BEVs play important roles in the microbial community interactions and host-microbe interactions. Markedly, BEVs can be delivered to host cells, thus modulating the development and function of the innate immune system. To clarify the compositions and biological functions of BEVs, we need to collect these vesicles with high purity and bioactivity. Here we propose an isolation strategy based on a broad-spectrum antimicrobial epsilon-poly-L-lysine (ϵ-PL) to precipitate BEVs at a relatively low centrifugal speed (10,000 × g). Compared to the standard ultracentrifugation strategy, our method can enrich BEVs from large volumes of media inexpensively and rapidly. The precipitated BEVs can be recovered by adjusting the pH and ionic strength of the media, followed by an ultrafiltration step to remove ϵ-PL and achieve buffer exchange. The morphology, size, and protein composition of the ϵ-PL-precipitated BEVs are comparable to those purified by ultracentrifugation. Moreover, ϵ-PL-precipitated BEVs retained the biological activity as observed by confocal microscopy studies. And THP-1 cells stimulated with these BEVs undergo marked reprogramming of their transcriptome. KEGG analysis of the differentially expressed genes showed that the signal pathways of cellular inflammatory response were significantly activated. Taken together, we provide a new method to rapidly enrich BEVs with high purity and bioactivity, which has the potential to be applied to BEVs-related immune response studies.
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2021
·
IF 5.268
Jingwen Wang,Jing Wang,Shuilai Qiu,Weijian Chen,Liang Cheng,Wenxiang Du,Jinghao Wang,Longfei Han,Lei Song,Yuan Hu
Biodegradable poly-(lactide-coε-caprolactone) (PLCL) scaffolds have opened new perspectives for tissue engineering due to their nontoxic and fascinating functionality. Herein, a black phosphorus-based biodegradable material with a combination of promising enhanced hydrophilicity , shape recovery and osteodifferentiation properties was proposed. First, amino black phosphorous (BP-NH 2 ) was prepared by a simple ball milling method. Then, L -lysine-modified black phosphorous ( L -NH-BP) was formed by hydrogen bonding between L -lysine and amino BP and integrated into PLCL to form PLCL/ L -NH-BP composite fibers . The scaffolds had excellent shape recovery and shape fixity properties. Moreover, based on gene expression and protein level assessment, the scaffolds could enhance the expression of alkaline phosphatase (ALP) and bone morphogenetic protein 2 (BMP2), simultaneously improving the mineralization ability of bone mesenchymal stem cells. Specifically, this new composite material was experimentally verified to be degradable under mild conditions. This strategy provided new insight into the design of multifunctional materials for diverse applications.
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DESALINATION
·
2020
·
IF 7.098
Zhengyang Gu,Shuili Yu,Junyong Zhu,Ping Li,Xuerui Gao,Ruijun Zhang
The utilization of metal-organic frameworks (MOFs) has been proved to be promising in the attainment of high-performance membranes. In this work, lysine-decorated UiO-66 (Lys@UiO-66) is prepared via post-synthetic modification. Then, the controlled positioning of Lys@UiO-66 on a polyethersulfone (PES) substrate membrane is achieved by a pressure-assisted method, followed by interfacial polymerization to fabricate the Lys@UiO-66-based thin-film nanocomposite membrane (TFN-LDU). An even distribution of hydrophilic MOFs atop the substrate induces the formation of regular tent-like and mesh-topology polyamide structures, which enhance the effective membrane surface area and provide additional water transport channels. The optimal membrane with a Lys@UiO-66 loading density of 17.13 μg cm −2 , TFN-LDU8, achieves a 55% enhancement in water permeability (18.27 L m −2 h −1 bar −1 ) without compromising the solute selectivity . Importantly, the TFN-LDU8 membrane exhibits high rejections of divalent cation salts (97.81% for MgCl 2 and 92.81% for CaCl 2 ) and improved mono-/divalent cation selectivity due to the pore hindrance and electrostatic exclusion of the Lys@UiO-66. Furthermore, the TFN-LDU8 membrane evinces a stable nanofiltration performance during the long-term nanofiltration experiment and displays a good antifouling ability in the fouling tests. This research provides a promising strategy for the design of nanofiltration membranes with special functions by using modified MOFs.
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JOURNAL OF FOOD ENGINEERING
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2023
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IF 5.5
Beibei Ye,Cikun Liu,Huan Li,Feifei Wang,Jian Chen,Yanbo Wang
The spoilage of seafood is highly dependent on temperature changes during cold chain. To visually reflect the shelf-life of large yellow croakers (0–25 °C), the xylose-lysine based time-temperature indicator (TTI) label was designed and applicated. The ultraviolet (UV) spectrum, and color response of Maillard reaction (MR) indicated that the color changes of TTI was related to the formation of melanoidins of MR. The color changes of TTI, and the quality degradation of large yellow croakers were described by kinetics analysis, which showed that Ea of TVB-N (70.45 kJ/mol) was closer to that of TTI (83.51 kJ/mol). Under fluctuating temperatures, the difference of the effective temperature (T eff ) between TVB-N (15.95 °C) and TTI (16.84 °C) was less than 1 °C, and the relative error of shelf-life was −8.33%. Briefly, this novel TTI label as an environmentally friendly system could be attached to sample surface for real-time reflecting shelf-life of seafood in cold chain.
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EUROPEAN POLYMER JOURNAL
·
2023
·
IF 6
Yao Xu,Bingtao Wang,Zhenghong Guo,Zhengping Fang,Peng Chen,Juan Li
A bio-based copolymer (TDDL) containing lysine, dopamine, triazine was synthesized using cyanuric chloride, dopamine and lysine, respectively. The TDDL was used as a charring agent and ammonium polyphosphate (APP) was used as an acid source to improve the flame retardancy of thermoplastic polyurethane (TPU). The flame retardancy and thermal degradation behaviors of TPU blends were investigated using UL-94 vertical burning, limiting oxygen index (LOI), cone calorimetry tests and thermal gravimetric analysis (TGA). TPU blend containing 8 wt% APP displays a UL-94 V0 rating and a LOI value 30.0 vol%. While TPU blend containing 6 wt% APP/TDDL displays a UL-94 V0 and a LOI 29.6 vol% suggesting good synergistic effect between TDDL and APP. In addition, TDDL reduces the heat release rate of TPU/APP blend, and promotes the formation of a perfect char layer during the combustion process, and suppresses the release of combustible gas and smoke. Moreover, TPU blends have better modulus and elongation at break than those of TPU.
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RSC Advances
·
2019
·Vol. 9
·期 35
·20044-20052
·
IF 3.049
Tianyu Liu,Dandan Chen,Feng Yang,Jinyao Chen,Ya Cao,Ming Xiang,Jian Kang,Ruizhang Xu
The application of a reverse osmosis (RO) membrane is greatly restricted on account of a trade-off between water flux and salt rejection, as well as poor anti-fouling properties. In order to improve the surface hydrophilicity of RO membranes, L-lysine (Lys) was grafted onto polyamide selective layers with the premise of maintaining the original surface morphology and thickness, which were verified through scanning electron microscopy and atomic force microscopy analysis. X-ray photoelectron spectroscopic measurements confirmed the occurrence of the reaction. After modification, the RO membranes exhibited notably enhanced hydrophilicity due to a steep decline in their water contact angles. The results of cross-flow filtration tests manifested that the modified membranes showed evident enhancements in water flux and salt rejection. For the optimized samples, the water flux increased by 22.45% compared with the pristine membrane, and the salt rejection rose up to 98.53% from the initial value of 95.44%. After grafting, the RO membranes performed much better in fouling resistance, especially towards protein and cationic foulants. The mechanism of how grafted Lys affected the performance of the RO membranes was analyzed. This research provides a feasible method to optimize the performance of RO membranes.
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Polymer Chemistry
·
2017
·Vol. 8
·期 45
·7033-7043
·
IF 5.375
Pan Li,Jiacheng Zhang,Chang-Ming Dong
A photoresponsive poly(o-nitrobenzyloxycarbonyl-L-lysine)-b-PEO (PNBL-b-PEO) polypeptide copolymer was synthesized and its photosensitive cleavage and self-assembly were monitored by means of UV-vis spectroscopy, 1H NMR, DLS, and TEM. Three kinds of dual-sensitive (i.e., photo/pH-thermo) polypeptide normal and reverse micellar hydrogels were then fabricated by the physical cross-linking of the self-assembled micelles. Utilizing the inclusion complexation between α-cyclodextrin (α-CD) and PEO of the normal micelles of PNBL-b-PEO or polylysine-b-PEO, photo/pH-thermo-sensitive normal micellar hydrogels were fabricated; in contrast, the reverse micelles of polylysine-b-PEO@α-CD self-assembled at pH = 3 and then were transformed into a pH-thermo-sensitive reverse micellar hydrogel at pH = 10. The multiple self-assembly and hydrogel properties were thoroughly characterized by means of UV-vis spectroscopy, circular dichroism (CD), wide angle X-ray diffraction (WAXD), DLS, zeta potential, rheology, TEM, and SEM. Notably, these dual-sensitive polypeptide hydrogels with a higher mechanical modulus (10–105 kPa) were facilely constructed and the mechanical modulus can be further tuned by varying the UV-irradiation time, the amount of α-CD added, and the copolymer concentration. Consequently, this work provides a versatile platform for constructing polypeptide copolymer hydrogels with tunable mechanical and stimuli-sensitive properties.
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FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Shuqi Hao,Mengyu Ye,Na Li,Zeyu Lu,Wei Quan,Huaide Xu,Mei Li
Advanced glycation end products (AGEs), a heterogeneous compound existed in processed foods, are related to chronic diseases when they are accumulated excessively in human organs. Protein-bound N ε -(carboxymethyl) lysine (CML) as a typical AGE, is widely determined to evaluate AGEs level in foods and in vivo . This study investigated the intestinal absorption of three protein-bound CML originated from main food raw materials (soybean, wheat and peanut). After in vitro gastrointestinal digestion, the three protein-bound CML digests were ultrafiltered and divided into four fractions: less than 1 kDa, between 1 and 3 kDa, between 3 and 5 kDa, greater than 5 kDa. Caco-2 cell monolayer model was further used to evaluate the intestinal absorption of these components. Results showed that the absorption rates of soybean protein isolate (SPI)-, glutenin (Glu)-, peanut protein isolate (PPI)-bound CML were 30.18%, 31.57% and 29.5%, respectively. The absorption rates of components with MW less than 5 kDa accounted for 19.91% (SPI-bound CML), 22.59% (Glu-bound CML), 23.64% (PPI-bound CML), respectively, and these samples were absorbed by paracellular route, transcytosis route and active route via PepT-1. Taken together, these findings demonstrated that all three protein-bound CML digests with different MW can be absorbed in diverse absorption pathways by Caco-2 cell monolayer model. This research provided a theoretical basis for scientific evaluation of digestion and absorption of AGEs in food.
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JOURNAL OF THE WORLD AQUACULTURE SOCIETY
·
2016
·Vol. 48
·期 2
·290-302
·
IF 0.665
Chenmin Liu,Yuzhe Han,Tongjun Ren,Zhiqiang Jiang,Fuqiang Wang,Mingling Liao,Jing Wang
A 56-d feeding trial was conducted to evaluate the effects of dietary lysine levels on growth performance, intestinal digestive enzymes, and nonspecific immune response of Apostichopus japonicus juveniles. Five semipurified diets were formulated to contain graded levels of lysine (from 0.28 to 2.32% of dry matter). Although no significant differences ( P > 0.05) were observed in survival rate, final weight (FW), and body weight gain (BWG), the FW and BWG increased from 0.28 to 1.19% with increasing dietary lysine levels, then showed a declining tendency. Broken-line regression analysis of BWG indicated sea cucumber requires lysine at 0.76% of dry diet. Proximate composition, lysine content of the body wall, and total amino acid profiles were not affected by dietary treatments. Intestinal protease level increased with increasing dietary lysine level from 0.28 to 1.19%, then decreased. Acid phosphatase and superoxide dismutase activities of the sea cucumbers were not affected by various dietary lysine levels; however, significantly ( P < 0.05) higher alkaline phosphatase activity was observed in sea cucumbers fed 2.32% lysine diet than those fed diets having 0.28 and 1.89% lysine. Overall, sea cucumber juveniles fed with diets containing between 0.76 and 1.19% lysine showed better growth performance, digestive enzymes, and nonspecific immune enzyme activities.
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