BIOMACROMOLECULES
·
2017
·
IF 5.246
Haijiao Zheng,Tenggao Zhu,Xiqian Li,Guan Wang,Qiong Jia
Biological-material-functionalized porous monoliths were prepared with lactoferrin and β-cyclodextrin via a click reaction. With the monolith as an extraction medium, a method combined with ICP-MS was developed for the determination of total gallium originating from metabolic residues of orally bioavailable gallium complexes with tris(8-quinolinolato)gallium (GaQ3) as a representative. The method exhibited favorable adsorption behaviors for gallium with high selectivity, low detection limit (2 ng L–1), and an enrichment factor of 29-fold with the sample throughput of 30 min–1. The developed approach was validated by the analysis of gallium from GaQ3 metabolic residues in a human cell line. Additionally, the practical applicability of this method was evaluated by the determination of gallium in human blood and urine samples from cancer patients. Results illustrated that the prepared monolith had potential in Ga-based anticancer drug analysis in complex biological samples.
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Stem Cell Research & Therapy
·
2020
·Vol. 11
·期 1
·1-15
·
IF 5.116
Shen Ying-Shan,Chen Xiao-Jun,Wuri Sha-Na,Yang Fan,Pang Feng-Xiang,Xu Liang-Liang,He Wei,Wei Qiu-Shi
Objectives Polydatin (PD), extracted from Polygonum cuspidatum , has shown potential therapeutic applications due to its antiosteoporotic and anti-inflammatory activities. Our previous study suggested that PD promotes the osteogenesis of human bone marrow stromal cells (hBMSCs) via the BMP2-Wnt/β-catenin pathway. The aim of our present study was to further explore the role of PD-mediated regulation of Tafazzin (TAZ), a transcriptional coactivator with a PDZ-binding motif, in osteogenesis. Materials and methods hBMSCs were isolated and treated with PD at various concentrations. Alizarin red staining and RT-qPCR were performed to identify calcium complex deposition in hBMSCs as well as the expression of specific osteoblast-related markers, respectively, in each group. Next, TAZ-silenced hBMSCs were generated by lentivirus-produced TAZ shRNA. After treatment with PD, the osteogenic abilities of the TAZ-silenced and control hBMSCs were estimated by ALP activity assay, and expression of the TAZ protein was detected by Western blot analysis and immunofluorescence staining. In vitro, an ovariectomized (OVX) mouse model was established and used to evaluate the effect of PD on bone destruction by micro-CT, immunohistochemistry, and ELISA. Results In vitro, 30 μM PD significantly improved the proliferation and calcium deposition of hBMSCs and markedly stimulated the expression of the mRNAs RUNX2 , Osteopontin , DLX5 , β-catenin , TAZ , and Osteocalcin ( OCN ). Osteogenic differentiation induced by PD was blocked by lentivirus-mediated TAZ shRNA. Furthermore, Noggin (a regulator of bone morphogenic protein 2 (BMP2)) and DKK1 (an inhibitor of the Wnt/β-catenin pathway) were found to inhibit the increase in TAZ expression induced by PD. In vivo, PD prevented estrogen deficiency-induced bone loss in the OVX mouse model. Conclusion Taken together, our findings suggest that PD improved the osteogenic differentiation of hBMSCs and maintained the bone matrix in the OVX mouse model through the activation of TAZ, a potential target gene of the BMP2-Wnt/β-catenin pathway.
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TALANTA
·
2018
·
IF 4.244
Miao Wang,Kun Su,Jing Cao,Yongxin She,A.M. Abd El-Aty,Ahmet Hacımüftüoğlu,Jing Wang,Mengmeng Yan,Sihui Hong,Shuibing Lao,Yanli Wang
Here, we developed a novel non-enzymatic rapid testing method for determination of organophosphate pesticide (malathion) in water. In principle, target molecule can block the Fluorescence resonance energy transfer (FRET) between chemical fluorescent probe (energy donor) and β-cyclodextrin-coated silver nanoparticles (@AgNP) (receptor). The effects of malathion on the dynamics of fluorescent probe and β-cyclodextrin@AgNP were evaluated and their properties were further characterized. The current methodology showed a good sensitivity of 0.01 μg/mL represented as a limit of detection (LOD) and the calibration curve was linear over the concentration range of 0.1–25 μg/mL. Recovery rate from water samples spiked at 3 different concentration levels (0.3, 0.4, and 0.6 μg/mL) showed satisfactory range between 83% and 101%.
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BIOORGANIC CHEMISTRY
·
2018
·
IF 3.929
Jianpeng Su,Jiayun Liu,Cheng Chen,Yuejuan Zhang,Kewu Yang
The superbug infection caused by New Delhi metallo-β-lactamase (NDM-1) has grown into an emerging threat, labelling and inhibition of NDM-1 has proven challenging due to its shuttling between pathogenic bacteria. Here, we report a potent covalent scaffold, ebsulfur, for targeting the protein in vitro and in vivo . Enzymatic kinetic study indicated that eighteen ebsulfurs gained except 1a – b and 1f inhibited NDM-1, exhibiting an IC 50 value ranging of 0.16–9 μM, and 1g was found to be the best, dose- and time-dependent inhibitor with an IC 50 of 0.16 μM. Also, these ebsulfurs effectively restored the antibacterial activity of cefazolin against E. coli expressing NDM-1, and the best effect was observed to be from 1g , 1i and 1n , resulting in an 256-fold reduction in MIC of the antibiotic at a dose of 16 μg/mL. The equilibrium dialysis study implied that the ebsulfur disrupted the coordination of one Zn(II) ion at active site of NDM-1. Labelling of NDM-1 using a constructed fluorescent ebsulfur Ebs - R suggested that the inhibitor covalently bound to the target through SDS-PAGE analysis in vitro . Also, labelling NDM-1 in living E. coli cells with Ebs - R by confocal microscopic imaging showed the real-time distribution change process of intracellular recombinant protein NDM-1. Moreover, the cytotoxicity of these ebsulfurs against L929 mouse fibroblastic cells was tested, and their capability to restore antibacterial activity of antibiotic against clinical strains E. coli EC08 producing NDM-1 was determined. The ebsulfur scaffold proposed here is valuable for development of the covalent irreversible inhibitors of NDM-1, and also for labelling the target in vitro and in vivo .
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 42
·64244-64251
·
IF 5.19
Zhao Ke-meng,Liu Ze-hua,Zhang Jun,Zhong Shu-shu,Dang Zhi
Arylsulfatase and β-glucuronidase are the two substantial enzymes having a significant role in the cleavage of conjugated natural estrogens (C-NEs). The present study reports that arylsulfatase and β-glucuronidase have been abundantly found in the digestive tracts of Cipangopaludina chinensis ; in which, their corresponding activities were 60 and 5 U/g wet waste, respectively. The arylsulfatase from Cipangopaludina chinensi s could show high activity at low temperatures. Hence, its activity still remained at 53.2% of maximal activity even at an extremely low temperature of 4 ℃; while the corresponding activities of arylsulfatase from Helix pomatia or activated sludge were less than 20% and 10%, respectively. The arylsulfatase and β-glucuronidase from Cipangopaludina chinensis could efficiently cleave C-NEs suggesting that they could be alternative enzymes derived from Helix pomatia that are used for cleavage of conjugated compounds in environmental or biological sample analysis. Meanwhile, they might also be used to enhance the cleavage of C-NEs in municipal wastewater.
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BEHAVIORAL ECOLOGY AND SOCIOBIOLOGY
·
2022
·Vol. 76
·期 12
·1-10
·
IF 2.944
Li Chengzhe,Wang Hua,Chen Xiaoming,Yao Jun,Deng Jianyu
Abstract Butterflies use visual and olfactory cues to identify conspecifics during courtship. Tirumala limniace , Idea leuconoe , and Danaus chrysippus are three species of butterflies with a sympatric distribution, and T. limniace butterflies are rarely seen to chase I. leuconoe or D. chrysippus during courtship in artificial cultivation gardens, indicating that they have a mechanism for recognizing conspecifics; however, there are no reports on what kind of signals they use to recognize conspecifics. In this study, we investigated the cues used by T. limniace to identify conspecifics and the relative roles of visual and olfactory cues. We found that in the absence of olfactory cues (visual cues only), male and female butterflies were able to identify the same species based on wing color and pattern. By collecting and identifying body volatiles emitted from T. limniace males and females, we found that the highest amounts were β -ocimene and β -pinene, which were sourced from the insects’ bodies (as opposed to sex pheromones). Furthermore, electroantennogram binding behavioral experiments showed that β -ocimene was involved in the process of species recognition and that the relative roles of olfactory cues were greater than those of visual cues at close range. Therefore, T. limniace males and females could utilize both visual and olfactory cues to identify conspecifics during courtship but preferred olfactory cues. The present study highlighted the important role of adult body odor rather than sex pheromones in butterfly species recognition. Significance statement Butterflies use visual (wing color and pattern) and olfactory (sex pheromones) cues to identify conspecifics during courtship. Butterflies also release volatiles emitted from their bodies during courtship. However, there is no clear evidence that body volatiles (as opposed to sex pheromones) emitted from butterflies are involved in species recognition. We studied the functions of body volatiles emitted from Tirumala limniace adults and the relative roles of visual and olfactory cues in species recognition during courtship. We found that male and female adults could identify conspecifics using only visual cues. Meanwhile, we also found that the highest amounts of β -ocimene, which came from the bodies of T. limniace males and females, were also involved in the process of species recognition. The olfactory cues that butterflies use to identify conspecifics are usually sex pheromones, while the present study highlighted the important role of adult body odor rather than sex pheromones in butterfly species recognition.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Shan-Shan Yang,Qing-Yun Lv,Juan Fu,Tian-Yi Zhang,Yi-Si Du,Xin-Juan Yang,Le Zhou
As our further research, a series of new 7-chloro-9-methyl-2-phenyl-3,4-dihydro-β-carbolin-2-iums were designed and synthesized. Twelve compounds were found with excellent inhibition activity in vitro on three to five out of six phytopathogenic fungi, superior to standard drugs thiabendazole and/or azoxystrobin. Especially, 18 displayed the highest activity against three out of the fungi and the highest comprehensive activity for all of the fungi. The test in vivo revealed that 18 at 50 μg/mL was able to completely control Physalospora piricola infections in apples over 8 days. Scanning/transmission electron microscopic observations found that 18 could damage the hyphal integrity and cell membrane structure of P. piricola. The safety evaluation showed that 18 had no effect on the germination rate of cowpea seed at ≤200 μg/mL. The SAR revealed that the combination of 7-Cl and 2′- or 4′-alkyl is conducive to improvement of the activity. Thus, 7-chloro-9-methyl-2-phenyl-3,4-dihydro-β-carbolin-2-ium is a promising antifungal lead scaffold.
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ANALYTICA CHIMICA ACTA
·
2013
·
IF 4.387
Haiyu Tian,Junhong Qian,Qian Sun,Hongyan Bai,Weibing Zhang
Three fluorescent probes were constructed by incorporating an α,β-unsaturated ketone to a coumarin fluorophore. The selective addition of sulfite to the alkene of TSP assisted by cetyltrimethyl ammonium bromide (CTAB) micelle can be visualized by dramatic color and ratiometric fluorescence changes. In CTAB–PBS system, the fluorescence intensity ratio at 465 nm and 592 nm ( I 465 / I 592 ) and the absorbance ratio at 390 nm and 470 nm ( A 390 / A 470 ) were linearly proportional to sulfite concentration in the range of 0.5–150 μM, and the detection limit was 0.2 μM. Good selectivity and competition of TSP1 towards sulfite over several anions and biological thiols were acquired. Probe TSP1 was used to detect sulfite in three realistic samples (mineral water, sugar and white wine) with good recovery.
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ENVIRONMENTAL RESEARCH
·
2019
·
IF 5.026
Xuejiao Hu,Yue Hu,Guizhou Xu,Meng Li,Yuanting Zhu,Lu Jiang,Yizhou Tu,Xingqi Zhu,Xianchuan Xie,Aimin Li
Adsorption is one of the most preferred techniques in the advanced treatment of dyeing wastewater. Magnetic porous materials with good adsorption performance, excellent reusability, and a green synthesis route are highly desirable adsorbents in commerce. In this study, we synthesized a magnetic β-cyclodextrin polymer (MNP-CM-CDP) containing many macro- and ultramicropores in aqueous phase. CO 2 adsorption-desorption isotherms and a dye adsorption method provided Langmuir specific surface areas for the MNP-CM-CDP of 114.4 m 2 g −1 and 153 m 2 g −1 , respectively. Model pollutants (BPA, MB , BO 2 , RhB , Cr(III), Pb(II), Zn(II), and Cu(II)) were rapidly and efficiently removed from the aqueous solution by the MNP-CM-CDP. In addition, the polymer could be easily separated from the solution under an external magnetic field. The adsorption of the contaminants was dependent on pH, while the effects of ionic strength and humic acid were slight in the concentration range studied. The polymer could be easily regenerated at room temperature and retained good adsorption performance. Moreover, the MNP-CM-CDP showed good feasibility for the removal of pollutants from actual dyeing wastewater samples.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2016
·Vol. 27
·期 8
·8254-8270
·
IF 1.798
Xi Junhua,Ding Mingye,Dai Jianbin,Pan Yajing,Chen Daqin,Ji Zhenguo
In this paper, Yb 3+ /Er 3+ co-doped hexagonal NaYF 4 have been successfully synthesized by solvothermal, thermal decomposition, hydrothermal and molten salt method, respectively. The crystal structures, particle morphologies, sizes and corresponding upconversion luminescent properties of β-NaYF 4 :Yb 3+ /Er 3+ nano/microcrystals are systemically studied. It is interesting to observed that the temperature-dependent UC luminescent properties of β-NaYF 4 :20 %Yb 3+ , 2 %Er 3+ samples are strongly related to their synthesis methods. Comparison of the upconversion luminescence and thermal sensitivity between the β-NaYF 4 :Yb 3+ /Er 3+ samples reveals that β-NaYF 4 :Yb 3+ /Er 3+ sub-microplates synthesized by thermal decomposition method have much stronger green emission intensity, better luminescent thermal stability and higher temperature sensitivity. This findings are relevant to the application of β-NaYF 4 :Yb 3+ /Er 3+ in optical temperature sensors and to the further understanding of the UC luminescent mechanism.
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APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Chongfei Yu,Pengyan Yang,Luna Tie,Siyu Yang,Shuying Dong,Jingyu Sun,Jianhui Sun
Herein, we demonstrate the design and fabrication of β-Bi 2 O 3 @ Bi 2 S 3 hollow microspheres with sheet-like hierarchical nanostructures targeting an advanced visible-light-responsive photocatalytic system. In comparison with pure Bi 2 S 3 and β-Bi 2 O 3 , the as-obtained β-Bi 2 O 3 @Bi 2 S 3 heterojunction photocatalyst exhibits a markedly enhanced sunlight photocatalytic activity towards the photo-degradation of rhodamine B. Following, the property of the β-Bi 2 O 3 @Bi 2 S 3 on the Cr 6+ photocatalytic reduction was then evaluated. Operating conditions such as β-Bi 2 O 3 @Bi 2 S 3 mass, tartaric acid concentration, and initial Cr 6+ concentration were analyzed. The results showed that a suitable reduction condition was selected as, β-Bi 2 O 3 @Bi 2 S 3 dosage 25 mg, initial concentration 20 mg·L −1 and molar ratio of Cr 6+ /tartaric acid 1:20. The outstanding photocatalytic performances of the hybrid photocatalyst are mainly attributed to the reduced recombination rates of photo-generated electrons/holes, enhanced light harvesting, increased active sites as well as the improved photo/chemical stability. Moreover, the photocatalytic mechanism of β-Bi 2 O 3 @Bi 2 S 3 for oxidation of RhB and reduction of Cr 6+ was investigated in detail. The enhanced photocatalytic activity and excellent chemical stability render the thus-fabricated heterostructure a promising candidate to remediate aquatic contaminants and meet the demands of future photocatalytic materials.
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Analytical Methods
·
2020
·Vol. 12
·期 19
·2434-2442
·
IF 2.596
Lian Wen,Peng Wu,Lei-Lei Wang,Li-Zong Chen,Ming-Lin Wang,Xia Wang,Jin-Ming Lin,Ru-Song Zhao
Covalent organic frameworks with tunable porous crystallinity and outstanding stability have recently exhibited fascinating pretreatment performance as solid-phase microextraction coatings. In this report, a β-ketoenamine-linked covalent organic framework (TpPa-1) was successfully constructed through a Schiff-base-type reaction between 1,3,5-triformylphloroglucinol (Tp) and para-phenylenediamine (Pa-1). A TpPa-1 coating was then fabricated on a stainless-steel fiber for capturing trace synthetic musks. This TpPa-1 coating exhibited strong interaction with synthetic musks because of its hydrophobicity and π–π affinity. This TpPa-1-based solid-phase microextraction methodology, coupled with gas chromatography-tandem mass spectrometry, provided high enrichment factors (1214–12 487), wide linearity (0.5–1000 ng L−1), low limits of detection (0.04–0.31 ng L−1), and acceptable reproducibility (relative standard deviation, <10%) for nine synthetic musks. Recoveries at three spiked levels in three types of water samples were between 76.2% and 118.7%. These results indicated the promising applicability of the TpPa-1 as a solid-phase microextraction fiber coating for reliably detecting trace concentrations of synthetic musks in the environment.
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