MICROCHIMICA ACTA
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2026
·Vol. 193
·期 5
·306
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IF 5.4
Zhu Yuchuan,Li Junhong,Yang Ruoxi,Bao Mingqiu,Hao Yakai,Chen Huijin,Yue Ruirui,Shu Jinbing,Duan Xuemin
A ternary nanocomposite, PEDOT:PSS/UiO-66-Cl@Ru, was successfully synthesized via a one-pot hydrothermal strategy for the ultrasensitive electrochemical detection of amaranth (AM) and Allura Red (AR). The conceptual advance of this work lies in the synergistic integration of halogen-induced intrinsic electroactivity, maximal atomic utilization of single-atom Ru, and the conductive bridging effect of PEDOT:PSS. This ternary design effectively overcomes the conductivity bottleneck of MOFs while delivering exceptional catalytic site density and enhanced interfacial stability. The resulting PEDOT:PSS/UiO-66-Cl@Ru platform delivered remarkable analytical performance, featuring wide linear ranges of 0.05–140 µM for AM and 0.07–50 µM for AR. The low limits of detection were 16.6 nM and 23.3 nM, with high sensitivities of 0.179 µA µM⁻¹ cm⁻² and 0.247 µA µM⁻¹ cm⁻² for AM and AR, respectively. Furthermore, the proposed sensor demonstrated excellent selectivity, reproducibility, and stability. Finally, the successful application of PEDOT:PSS/UiO-66-Cl@Ru in detecting AM and AR within beverage samples validates its practical utility for on-site food safety screening.
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Nature Protocols
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2026
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IF 18.4
Cao Fan,Wang Sheng,Chen Zhixin,Zhang Shizheng,Wu Qianqian,Zhang Jiaqi,Yang Xuyong
Quantum dots (QDs) have emerged as promising candidates for next-generation display owing to their exceptional optoelectronic properties. However, despite substantial advancements in QD synthesis, the blue-emitting QDs, especially heavy-metal-free blue ones, still underperform compared with their red and green counterparts. ZnSeTe QDs offer a viable ecofriendly alternative for blue emissions, but their performance is limited by spectrum broadening (linewidth >20 nm) and structural instability. These issues stem from compositional inhomogeneity, which is primarily induced by Te aggregation during synthesis. Recently, we realized the synthesis of homogeneous quaternary-alloyed ZnSeTeS QDs through a synergistic strategy of reactivity modulation and isoelectronic control. This Protocol enables precise bandgap tuning in the blue spectral region (450–475 nm) by controlling the Te ratio, while ensuring high color purity and stability of QDs. Furthermore, the as-prepared ZnSeTeS QDs exhibit outstanding electroluminescence performance, with a peak external quantum efficiency of 24.7% and half-life of 29,600 h at 100 cd cm −2 , and demonstrate strong potential for applications such as solid-state lighting and bioimaging owing to their high stability and low toxicity. Here we detail a synthesis Protocol for ZnSe 0.94 Te 0.03 S 0.03 /ZnSe/ZnS core/shell/shell QDs via a hot-injection method using zinc carboxylate and anionic phosphine precursor, systematically outlining the design and preparation of precursors and QDs, post treatments (including purifications) and characterization methods, including time-resolved photoluminescence spectroscopy. The entire process typically requires 11–12 h for QD synthesis and 6 h for characterizations, demanding only accessible chemistry knowledge and routine colloidal synthesis techniques.
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Scientific Reports
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2026
·
IF 4.9
Zhu Lian,Liu Jin,Li Kailin,Wang Yang,Liu Fengjiao,Xie Liu,Zhang Yan,Cai Wei
Tryptophan is an essential amino acid for the human body, and its metabolic processes are closely related to a variety of physiological and pathological processes, including neurodegenerative diseases, immune modulation, and cardiovascular diseases. Simultaneous detection of tryptophan and its nine metabolites is challenging and previous methods may have shortcomings of poor sensitivity, selectivity, and specificity in complex matrices. The technology of LC-MS/MS, with its characteristics of high sensitivity and selective, provides a powerful tool for the detection of biomarkers. A rapid and effective method for the simultaneous determination of kynurenine metabolites (Xanthurenic acid (XA), DL-Tryptophan (TRP), Kynurenine (KYN), 3-Hydroxyanthranilic acid (3-HAA), Quinolinic acid (QUIA), Kynurenic acid (KYNA), 3-Hydroxy-DL-kynurenine (3HK), 2-Picolinic acid (2PA), 5-Hydroxytryptamine (5-HT), and Anthranilic acid (AA)) was established in human plasma using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technology. Methanol precipitation was used for sample pretreatment, with [2H5]-L-Tryptophan (TRP-D5) as the internal standard. Mass spectrometric detection was performed in positive electrospray ionization mode using multiple reaction monitoring on a triple-quadrupole mass spectrometer. The method’s selectivity, linearity, quantification limit, detection limit, precision, recovery rate, matrix effect, and stability are evaluated. The results show that the linear ranges of 2PA, QUIA, 3HK, 5HT, KYN, 3HAA, TRP, XA, KYNA and AA are 0.25-50, 0.9–225, 1.2–120, 1.4–700, 2-2000, 0.15–150, 180-180000, 0.1–100, 0.1–100, and 0.2–100 ng/mL, respectively; all analytes have good linear relationships with a determination coefficient R2 greater than 0.99. The precision relative standard deviation (RSD) is less than 15%; the spiked recovery rate is 87.54%-107.43%, with an RSD of 2.76%-12.19%. Additionally, the matrix effects for 10 analytes are summarized. At all three QC levels, the matrix effect in plasma is 88.44% to 107.85%, and the RSD is 2.97%-14.62%. These results indicate that there is no significant matrix effect. Stability was evaluated by comparing the measured concentrations after storage with the initial concentrations and expressed as percentages of the initial values. All analytes remained within 85–115% of the initial concentrations under the tested conditions. This study describes the development and validation of a rapid, sensitive, and robust UHPLC–MS/MS method for the simultaneous quantification of ten kynurenine pathway metabolites—including the precursor tryptophan, key intermediates, and neurotoxic/neuroprotective end products-in human plasma. The method employs a simple protein precipitation procedure, a single deuterated internal standard (TRP-D5), and a 13-minute chromatographic run on a C8 column, providing adequate separation of metabolites with diverse physicochemical properties.
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Nature Communications
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2026
·
IF 18.1
Feng Weixiao,Li Peifan,Li Xin,Wang Ziwei,Chen Min,Hu Yang,Xu Fu-Jian,Shi Shaowei
Bottom-up assembly of protocell building blocks into self-supporting, macroscopic, and robust prototissues that are stable in water and exhibit biomimetic behaviors remains a fundamental challenge. Here, we present a gas-liquid microfluidics-assisted diffusion-inhibited complexation strategy that enables low-cost, high-throughput fabrication of protocells and precise, large-scale (>10 cm) 3D construction of prototissues. The resulting prototissues display good mechanical integrity in both water and air and resist disintegration under external perturbations. By modulating the surrounding matrix or integrating bioactive components into the prototissue framework, programmable deformation/motion can be achieved through chemo-mechanical transduction. This strategy provides a platform for modeling the intricate behaviors of living systems and may facilitate applications in synthetic biology and bioengineering.
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JOURNAL OF NANOBIOTECHNOLOGY
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2026
·Vol. 24
·期 1
·269
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IF 15
Fan Yonggang,Wang Ze-Han,Zhang Xu,Dong Yu,Liu Xin,Luo Gan,Yang Houzhi,Li Huinan,Wang Zicheng,Tang Qiong,Jin Xin,Guo Dong-Sheng,Sun Tianwei
Current clinical management of fractures relies largely on surgical intervention and systemic pharmacotherapy, both of which are frequently associated with side effects and largely neglect early microenvironmental abnormalities. Precise elimination of senescent cells at the early stage may offer a more efficient means of restoring microenvironmental homeostasis and resetting regenerative dynamics. To this end, we designed and synthesized a multifunctional supramolecular nanoplatform, Asp 10 SAC4A, based on combination of host–guest recognition and self-assembly to simultaneously address three fundamental challenges in selectively eliminating senescent cells at the fracture site: inadequate targeting, insufficient responsiveness, and poor coordination of multi-drug delivery. The platform consists of an azocalix [ 4 ]arene modified with a deca-aspartate (Asp 10 ) peptide for selective binding to newly exposed hydroxyapatite at fracture sites, enabling active localization. Simultaneously, Dasatinib and Quercetin are co-loaded in a controlled ratio, and triggered for rapid release through the hypoxia-responsive mechanism of the azo groups, achieving microenvironment-specific “source-clearing” therapy. This strategy significantly alleviates inflammation, enhances osteogenesis and accelerates fracture healing. These results highlight the crucial role of selectively targeting senescent cells in the early phase of fracture and offer an innovative therapeutic approach that improves treatment efficiency and prognosis. Overall, this study aims to establish a fracture-site–activated senolytic delivery strategy that integrates fracture targeting, hypoxia-triggered release, and combination therapy, thereby enabling precise early-stage anti-senescence intervention to accelerate fracture healing. Graphic abstract
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Advanced Fiber Materials
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2026
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IF 22.6
Yao Yijun,Wei Xin,Ge Huixin,Zhou Jinyu,Zhang Pengfei,Xue Ying,Zhou Dan,Sheng Cuihong,Zhu Lingfeng,Xie Qiyuan,Hu Linlin,Shen Guodong,Wu Hailiang,Zhang Zhenfang,Xia Caijuan,Ramakrishna Seeram
The mechanoluminescent (ML) fiber, as a unique type of self-powered material, has become an important material in fields such as visual sensing, flexible displays, and smart wearables. However, their non-continuous processability, low response sensitivity, and limited luminescence capacity severely restrict the development of existing ML fibers for smart wearable electronic textiles. Herein, for the first time, we present an interfacial fusion engineering strategy that enables single-step, large-scale continuous fabrication of micron-sized ML fibers with high sensitivity and durability via hydrogel-assisted coaxial wet spinning. The introduction of temperature-responsive hydroxypropyl cellulose (HPC) significantly enhanced the interfacial fusion between the core and sheath layers, endowing the fibers with high stress (5.18 MPa), large strain (150%), and excellent toughness (195.57 J/m 3 ). Notably, the ML fiber showed an ultra-low strain threshold (3.3%) and high luminescence sensitivity ( K = 318.18), maintaining > 90% of its luminescence intensity after 5000 stretch–release cycles. The ML fibers exhibit luminescence behavior superior to that of previously reported coated fibers. Theoretical calculations suggest that HPC enhances luminescence through reduced electron transition energy and accelerated carrier separation with directional transfer. Moreover, integration with conductive fibers endows the ML fiber with triboelectric sensing capabilities for human motion monitoring. By enabling continuous fabrication of highly sensitive, durable, and bright stress-luminescent fibers with triboelectric sensing, this work opens new avenues for scalable production of multifunctional smart fibers for advanced wearable electronics.
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Science China-Materials
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2026
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IF 7.7
Zhou Qun,Guo Junze,Wen Xian,Dong Yalin,Sun Zhaoyang,Qin Kunlin,He Xinyang,Zhang Hongnan,Wang Liming,Qin Xiaohong
Emerging moisture-enabled energy harvesting technologies offer a promising route for self-powered strain sensing, yet conventional generators suffer from slow response, poor recovery, and limited multidirectional resolution. Here, we report a stretchable thermoplastic polyurethane (TPU) nanofiber moisture-enabled electric generator (MEG) with highly aligned ion channels. A carbon black/sodium dodecylbenzene sulfonate (CB/SDBS) layer is coated on the TPU membrane, while carboxymethyl cellulose (CMC) and acidified poly(sodium 4-styrenesulfonate) (HPSS) are applied on opposite sides, establishing lateral hydrophilicity and ion gradients to drive directional ion migration. The planar MEG is lightweight, flexible, and requires no fully covered electrodes, enabling conformity to complex deformations. The aligned channels reduce ion migration tortuosity, enhancing ion transport efficiency and flux. As a result, the aligned MEG (ATMEG) delivers 0.2 V and 0.51 µA cm −2 at ∼90% relative humidity, corresponding to 400% and 287% enhancements compared with the unaligned MEG (UATMEG). The ATMEG also exhibits ultrafast response (0.16 s) and recovery (0.08 s). Utilizing its anisotropic characteristics, a multidirectional self-powered strain sensor is developed, capable of distinguishing both the amplitude and direction of human motion, demonstrating strong potential for adaptive wearable electronics and intelligent motion monitoring.
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INTERNATIONAL UROLOGY AND NEPHROLOGY
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2026
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IF 2.2
Liu Hong,Zhang Shuang,Zhao Xiu‑Nan,Wang Zhen‑Zhen,Song Jia‑Ni,Liu Shu‑Xin
Purpose Membranous nephropathy (MN) is the most common pathological type of primary nephrotic syndrome in adults. MicroRNAs (miRNAs) are involved in the pathogenesis of several renal diseases. Here we investigated the potential involvement of miRNAs in MN progression. Methods miRNA sequencing was performed in the urinary exosomes of MN patients and normal controls (three cases per group). An experimental mouse MN model induced by cationic bovine serum albumin (CBSA) and MPC5 podocytes intoxicated by puromycin aminonucleoside (PAN) were used in this study. Results Compared with the normal controls, 19 miRNAs were downregulated and 13 upregulated in the urinary exosomes of MN patients. In an expanded cohort, we confirmed that miR-423-5p was significantly upregulated in the urinary exosomes of MN patients. Urinary particle concentrations and size distribution were assessed using transmission electron microscopy and NanoSight. Similarly, upregulated miR-423-5p was observed in PAN-intoxicated MPC5 cells. Lentiviral vector-mediated inhibition of miR-423-5p suppressed podocyte apoptosis and inactivated β-catenin signaling. Upon miRNA database prediction, WT1 was identified as a target of miR-423-5p. Consistently, downregulated WT1 expression in PAN-intoxicated MPC5 cells was rescued by miR-423-5p inhibition. The target relationship between miR-423-5p and WT1 mRNA 3’UTR was validated by the dual luciferase reporter assay. Knockdown of WT1 reversed the inhibitory effect of LV-anti-miR-423-5p on podocyte apoptosis and β-catenin signaling. Notably, miR-423-5p inhibition and WT1 overexpression attenuated renal injury and podocyte apoptosis in MN mice. Conclusion Our study demonstrates that miR-423-5p expression is upregulated in urinary exosomes of MN patients and modulates WT1, affecting podocyte apoptosis by the β-catenin signaling pathway. Inhibition of miR-423-5p action may protect against MN.
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Nature Communications
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2026
·
IF 18.1
Fang He,Shuye Ying,Haiyu Liu,Quan Wan,Yajun Shuai,Jie Wang,Ming Liu,Mingying Yang,Zongpu Xu
Developing polymer matrices with closed-loop recyclability is a key solution to solve the sustainability issues caused by unrecyclable end-of-life multi-phase composites, and using building blocks from renewable resources rather than petrochemical products presents an attractive option. However, the strategy of first extracting monomers from biomass feedstocks and then polymerizing them is functionality-removed, complexity-increased, and energy-inputted. We report a strategy demonstration from current polymer-monomer-matrix mode to polymer-macromolecule-matrix, based on retaining the advantages in property, processing, and recycling of silk protein. The silk matrix as was its fiber-reinforced composite show spontaneously room-temperature molding and curing capabilities, totally avoiding the use of any additives. The obtained composites also possess high mechanical property and environmental durability. Moreover, the multicycle recyclability of composites could be achieved by a simple room-temperature dissolution procedure, acquiring lossless reinforcing fibers and reusable silk protein. Conventional carbon fiber composites require high temperature curing and have complex recycling procedures. Here, the authors report high strength silk fibroin carbon fiber composites which are synthesized and recycled via room-temperature solvent-based methods.
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JOURNAL OF NANOBIOTECHNOLOGY
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2026
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IF 15
Wei Zhang,Yanan Zhou,Zijun Li,Zhaowen Wang,Fangzheng Zhu,Yaoyu Zou,Nianshuang Li,Cong He,Nonghua Lu,Xiaolei Wang,Yin Zhu
Helicobacter pylori (H. pylori) infection has emerged as a serious risk factor for global human health. Current antibiotic-based clinical eradication therapies still have many challenges, including inadequate drug permeability across the gastric mucus barrier, H. pylori biofilm-induced antibiotic resistance and recurrent infections, as well as gut dysbacteriosis. Herein, we report a probiotic-nanozyme hybrid system (LP@FeTA/CuPt), which is formed by coupling polyphenol-coated Lactobacillus plantarum (LP@FeTA) and CuPt nanozymes. LP@FeTA effectively penetrates the gastric mucus barrier via autokinetic movement, and facilitates the targeted delivery of CuPt nanozymes within H. pylori biofilms by "eating" extracellular polymeric substances (EPS). CuPt nanozymes respond to the acidic and H 2 O 2 -rich biofilm microenvironment to generate reactive oxygen species (ROS) and release Cu ions, thereby achieving multi-target eradication of biofilms by interfering with H. pylori flagellar self-assembly, urease activity, outer membrane function and energy metabolism. Importantly, LP@FeTA/CuPt can be degraded by transferrin at the infection site, allowing the released CuPt nanozymes to reactivate the antibacterial immune functions of macrophage, thus eliminating biofilm-escaped bacterioplankton to prevent recurrent infections. Notably, LP@FeTA/CuPt also modulates gut microbiota homeostasis. This study provides a promising non-antibiotic therapeutic strategy for H. pylori infection.
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NEUROCHEMICAL RESEARCH
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2026
·Vol. 51
·期 2
·129
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IF 4.5
Liu Shanshan,Yan Jing,Dong Jian
This study investigates the early synergistic effects of curcumin and licorice through vertical cooperation, which can simultaneously target both the upstream and downstream components of neuroinflammation. It evaluates their neuroprotective effects and potential mechanisms in a d -galactose/sodium nitrite-induced Alzheimer’s disease mouse model.Eighty C57BL/6 mice were divided into eight groups ( n = 10): wild-type (WT), AD model, curcumin monotherapy (AD + CL, 100 mg/kg), G. glabra monotherapy (AD + GG, 100 mg/kg), low-dose combination (AD + COM-L, 50 + 50 mg/kg), high-dose combination (AD + COM-H, 100 + 100 mg/kg), donepezil (3 mg/kg), and SN50 (NF-κB inhibitor, 400 µg/kg). Cognitive function was assessed via Morris Water Maze in WT, AD, AD + CL, AD + GG, and AD + COM-H groups, while all groups underwent molecular analyzes. The high-dose combination most effectively restored spatial memory, reducing escape latency by ~ 43% versus monotherapies. Molecularly, it synergistically reduced tau-related proteins (MAPT, GSK-3β) and suppressed the TLR4/MyD88/NF-κB axis, lowering inflammatory mediators (IL-6, TNF-α, CXCL1, PTGS2). IL-6 was further reduced by 28.6% and 40.0% compared to curcumin and G. glabra alone, respectively. The combination also enhanced antioxidant defense (increased SOD) and anti-apoptotic capacity (upregulated BCL-2) while reducing oxidative lipid damage (lower MDA). Network pharmacology identified 40 shared AD targets, with enrichment in NF-κB and IL-17 pathways, validated experimentally. In conclusion, curcumin and G. glabra exert synergistic neuroprotection by concurrently inhibiting the TLR4/NF-κB pathway and modulating IL-17 signaling, with G. glabra potentially targeting the TLR4/HMGB1 axis and curcumin directly inhibiting NF-κB activation, forming a complementary mechanistic interplay. This multi-target action disrupts the interplay between neuroinflammation and tau pathology, underscoring the combination’s therapeutic potential for AD intervention.
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CELLULOSE
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2026
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IF 4.6
Su Zhongyong,Li Jin,Lai Junjie,Liu Kai,Li Jianguo,Wang Qiang,Huang Liulian,Huang Fang,Miao Qingxian
The global scarcity of freshwater resources has become increasingly critical in recent years, highlighting the urgent need to develop efficient and energy-saving solar evaporative materials for seawater desalination and effluent purification. In this study, pulp fiber was employed as a raw material without any chemical pretreatment to prepare an aerogel evaporator through a one-step freeze-casting process. This approach offers process simplicity, cost-effectiveness, facile microstructure regulation, and excellent evaporation performance. The aerogel possesses a multidimensional layered structure with interconnected macropores, as well as excellent mechanical stability and water transport ability, which is attributed to the abundant hydroxyl groups. The incorporation of carbon nanotubes (CNTs) significantly improves photothermal absorption ability and conversion efficiency. The evaporator delivers a remarkable evaporation rate of 1.91 kg·m − 2 ·h − 1 and a high energy conversion efficiency of 92% under one-sun irradiation with simulated seawater. Furthermore, driven by capillary forces and ion migration, the aerogel generates a voltage of 532.45 mV, a current of 240.64 μA, and an output power of 24.12 μW·cm − 2 , demonstrating promising capability for water-electricity cogeneration. Additionally, the aerogel evaporator exhibits remarkable salt resistance, cycling stability, and outdoor adaptability, as well as effective purification of high-salinity seawater and organic dye-contaminated effluent. This work offers a new alternative for the high-value utilization of biomass resources and structural optimization of solar interfacial evaporator. Graphical abstract
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