EUROPEAN JOURNAL OF MEDICAL RESEARCH
·
2026
·
IF 4.8
Li Chenhao,Wang Xin,Shi Dongliang,Yang Meng,Yang Wenhua,Chen Liang
Background Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer. Tumor cells often promote the recruitment and infiltration of tumor-associated macrophages (TAMs). We focused on the effect of ETS-like transcription factor 4 (ELK4) on macrophage infiltration in promoting TNBC progression. Methods The publicly available TNBC dataset (GSE38959) was analyzed and found that ELK4 is significantly upregulated in TNBC tissues. Furthermore, Transwell, CCK-8, clonal formation, and EdU assay examined the regulation of ELK4 on cell invasiveness and proliferation. TNBC patient M2 infiltration was detected by flow cytometry. Dual-luciferase assay and ChIP-qPCR experiments were used to assess the bond between ELK4 and Muscleblind Like Splicing Regulator 1 (MBNL1). Results Analysis of the GSE38959 database indicated that ELK4 expression was upregulated in TNBC. Survival analysis revealed that higher ELK4 expression was associated with poorer prognosis in TNBC patients. Clinically, immunohistochemical staining of ELK4 in 60 TNBC patients’ samples suggested that higher ELK4 expression was associated with advanced TNM stage. Double-immunofluorescence staining for ELK4 and CD68 in TNBC tissues revealed a positive correlation between ELK4 expression and the number of CD68 + M2 macrophages. In vitro experiments, ELK4 overexpression enhanced the proliferation, colony formation, and the migration and invasion ability of TNBC cells. ELK4 knockdown showed opposite effects. Macrophage migration and M2 polarization were promoted when cultured in the conditioned medium derived from ELK4-overexpressing TNBC cells and inhibited in the conditioned medium derived from ELK4-silenced TNBC cells. Furthermore, ELK4 promoted Notch signaling activation in TNBC cells. Notably, ELK4 bound to the MBNL1 promoter and downregulated its activity, which was confirmed via dual-luciferase reporter assay and ChIP-qPCR. Conclusion Taken together, our results demonstrate that ELK4 may affect the Notch1 signaling pathway and promote macrophage M2 infiltration through transcriptional inhibition of MBNL1, ultimately promoting TNBC development.
✓ 已验证
DOI
高影响因子
AAPS PHARMSCITECH
·
2026
·Vol. 27
·期 3
·177
·
IF 5.1
Xu Fanjun,Li Meiting,Ruan Hang,Guo Teng,Wang Zhi,Feng Nianping,Zhang Yongtai
Retinol has emerged as a star ingredient in the cosmetics industry owing to its remarkable skincare efficacy. However, its major limitations—high irritation potential and chemical instability—necessitate further improvement. We developed a liposome primarily composed of glyceryl monooleate and poloxamer (F127). By hybridizing this with a binary alcohol system comprising a 1:1 (v/v) mixture of propylene glycol and dipropylene glycol, an ethosome (ES) capable of efficiently encapsulating retinol was obtained. Retinol-loaded ES (Ret-ES) was further modified with D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS@Ret-ES), thereby optimizing particle size distribution and drug loading capacity. Increasing the binary alcohol concentration from 10 to 30% caused TPGS@Ret-ES hydrated particle size to sharply decrease from 100 to 50 nm, without significant changes in drug loading or encapsulation efficiency. Compared with retinol aqueous solutions, TPGS@Ret-ES substantially reduced degradation rates at room temperature while maintaining excellent particle size stability. Additionally, incorporating antioxidants tocopheryl acetate and Irganox 1010 further improved chemical stability. Notably, TPGS@Ret-ES simultaneously enhanced transdermal drug permeation and skin retention, with no significant irritation observed following repeated application to the same skin site in guinea pigs. In conclusion, ES represents a highly promising topical delivery carrier, and TPGS@Ret-ES shows considerable potential as a novel formulation for retinol. Graphical
The alternative text for this image may have been generated using AI.
✓ 已验证
DOI
PubMed
高影响因子
ARCHIVES OF TOXICOLOGY
·
2026
·
IF 10.9
Deng Li,Yan Hanwen,Qian Zhipeng,Zhong Jiaqi,Xie Wenpei,Wang Jianfeng,Xu Jun,Gao Hong,Zong Liang,Chen Xijing,Huang Haoyan,Zhang Yongjie
Chlorfenapyr (CFP) is a halogenated pyrrole insecticide that requires metabolic activation to exert toxicity and has been associated with severe poisoning in humans. However, the metabolic pathways underlying CFP bioactivation and detoxification in humans remain poorly defined. In this study, we systematically investigated the metabolic fate of CFP and its toxic metabolite tralopyril (TLP) using in vitro human drug-metabolizing enzyme systems. Screening of ten human cytochrome P450 (CYP) isoforms at 5 and 50 µM CFP indicated that CYP2B6 is the major contributor to CFP bioactivation, which was further supported by selective inhibition and correlation analyses. CFP metabolism by CYP2B6 followed Michaelis–Menten kinetics, with showing a K m of 1.80 ± 0.57 μM, a V max of 0.24 ± 0.02 pmol/min/pmol P450, and an CL int of 139.78 ± 33.70 nL/min/pmol P450. In addition, incubations using 50 or 100 µM TLP and varying concentrations of glutathione (0.1 to 1 mM GSH) showed the formation of a novel GSH conjugate of TLP (TLP-GSH), of which level was markedly enhanced by human glutathione S-transferases (GSTs). Among six recombinant human GST isoforms tested, GSTA1, GSTA2, GSTM1, and GSTP1 showed significant catalytic activity in TLP-GSH formation compared with non-enzymatic reactions. Moreover, extracellular flux analysis revealed that TLP dose-dependently inhibited basal and maximal oxygen consumption rates, an effect exacerbated by GST inhibition, indicating a protective role of GSTs in TLP-induced mitochondrial toxicity. Together, these findings delineated metabolic enzymes-mediated bioactivation and detoxification pathways governing CFP toxicity in humans and provided a new perspective on clinical management strategies for CFP poisoning.
✓ 已验证
DOI
PubMed
高影响因子
BMC BIOTECHNOLOGY
·
2026
·Vol. 26
·期 1
·34
·
IF 4.8
Huo Huanglin,Zhou Zhenhua,Guo Rongxiang,Zhang Jinyi,Li Menghan,Zeng Huicong,Zhao Dongyu,Terekhov Roman P.,Jiang Peng,Zhou Qian,Li Bo
Diabetes wounds represent a significant clinical challenge, primarily attributed to their complex microenvironment characterized by persistent inflammation, heightened infection susceptibility, and impaired angiogenesis. To address these interconnected challenges, a multifunctional hydrogel dressing (designated EEP/PS) was constructed using pullulan, Brazilian propolis ethanolic extract, and polyvinyl alcohol. This biomaterial exhibits an interconnected porous structure, suitable mechanical properties, tissue adhesion, remarkable thermal stability, excellent sustained-release and swelling capacity. Notably, EEP/PS shows potent antioxidant capability and significantly enhances cell migration in hydrogen peroxide-induced oxidative injury models. Furthermore, the hydrogel downregulates the expression and secretion of key pro-inflammatory cytokines in LPS-stimulated macrophages, including tumor necrosis factor-α, interleukin-6, and interleu-kin-1β. The hydrogel also exhibits broad-spectrum antibacterial efficacy against common wound pathogens Escherichia coli and Staphylococcus aureus. In a diabetic rat model, EEP/PS accelerated wound closure by synergistically promoting reepithelialization, collagen deposition, angiogenesis while mitigating local inflammation. Additionally, EEP/PS exhibits an excellent hemostasis capacity and bio-compatibility, critical for clinical translation. Collectively, these findings highlight the translational potential of EEP/PS as a multifunctional biomaterial for diabetic wound care.
✓ 已验证
DOI
高影响因子
Nature Communications
·
2026
·
IF 18.1
Hao Shuaipeng,Zhang Yi,Liu Shuaiwei,Tan Zhouliang,Liu Wei,Xia Yuanguang,Yin Wen,Liao Yaqi,Ji Haijin,Kong Yuelin,Shao Yudi,Lv Yuelin,Qie Long,Huang Yunhui,Yuan Lixia
Direct regeneration offers a shortcut to close the material supply loop of lithium-ion batteries and is a promising recycling strategy. However, in spent LiNi 0.5 Co 0.2 Mn 0.3 O 2 positive electrode, severe bulk cation disorder and surface rock salt phase hinder Li + reinsertion. Moreover, the coexistence of single and poly-crystal particles in commercial batteries further complicates uniform re-lithiation and morphological regeneration. Herein, we propose an oxidation strategy to simultaneously regulate the structural reconstruction and morphological evolution of spent material. During oxidation, surface NiO transforms into NiOOH, while targeted oxidation of the anti-site Ni 2+ to Ni 3+ in the bulk reduces Li + /Ni 2+ mixing. This reconstructs Li + diffusion channels from surface to bulk, facilitating re-lithiation. Meanwhile, structural changes induce lattice expansion in secondary particles, causing their decomposition into primary particles and forming uniform precursor particles. These particles, with continuous Li + transport channels and NiOOH surface, agglomerate into large single-crystal during calcination. The regenerated LiNi 0.5 Co 0.2 Mn 0.3 O 2 achieves a high tap density of 2.57 g/cm 3 and retains 80.2% capacity after 600 cycles. This work presents a concept for the direct regeneration of degradable positive materials.
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 5
·310
·
IF 5.4
Lu Xia,Wang Long,Gao Qinyi,Zhang Xianglin,Wang Yuxin,Wang Fang,Yang Xujin,Ge Wupeng
A novel fireproof material@UIO-66-gold nanoparticles (AS@UIO-66-AuNPs) fiber network using low-cost fireproof material (AS) via an in-situ synthesis strategy has been developed. This functionalized network anchors specific aptamers (Apt), creating a highly efficient aptamer affinity column for the sensitive detection of aflatoxin B1 (AFB1) in food samples. Notably, this work focuses on the construction of an aptamer-based affinity pretreatment column rather than a signal-output sensing platform, aiming to improve target enrichment and matrix cleanup prior to instrumental analysis. Under optimized conditions, the method demonstrated excellent analytical performance, with a linearity range of 1–100 µg/kg and a detection limit of 0.157 µg/kg. Validation with spiked recovery experiments on corn, soybean meal, and rapeseed meal showed satisfactory recoveries and strong agreement with a commercial detection kit. The method also exhibited excellent reproducibility and reusability. Overall, the AS@UIO-66-AuNP/Apt system represents a cost-effective, stable, and selective strategy for the pretreatment and enrichment of AFB1, with potential applicability to other targets through aptamer replacement, thereby supporting the development of multifunctional aptamer affinity columns. Graphical abstract The alt text for this image may have been generated using AI.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2026
·
IF 4.4
Haoting Niu,Shuo Tang,Moyong Li,Lilu Guo,Lulu Qin,Liuyun Jiang,Anji Chen,Xiang Hu
A novel multifunctional double-network hydrogel composed of carboxymethyl chitosan-oxidized sodium alginate-poloxamer (PF127) loaded with Panax notoginseng saponins (OSA/CM/PF127@PNS) was developed for intrauterine adhesion therapy. FT-IR spectroscopy confirmed acetalation crosslinking between OSA and CMC, as evidenced by characteristic imine peak (1636 cm⁻ 1 ). SEM observations revealed the hydrogel possessed a porous microstructure, with robust self-healing capability validated (by rheological cyclic strain tests), a maximum swelling ratio of 1155%, and sustained PNS release reaching 52.2% at 24 h. In vitro degradation studies indicated the stability under physiological pH (7.4) was enhanced due to the dual-crosslinked network. Moreover, the hydrogel exhibited potent antibacterial activity (> 90% inhibition against E. coli and S. aureus ), antioxidant capacity, and excellent hemocompatibility (< 5% hemolysis). The hydrogel showed exceptional biocompatibility in cellular assays. Notably, PNS loading significantly suppressed pro-inflammatory cytokines (TNF- α , IL-1 β , IL-6) at the mRNA level and inhibited NF- κ B signaling at the protein level. Furthermore, it promoted angiogenesis in vitro, evidenced by upregulated angiogenesis-related proteins. Therapeutically, the hydrogel significantly accelerated wound healing in a murine model (> 90% closure by day 9) and effectively promoted endometrial regeneration in a rat IUA model. This work provides a novel and promising strategy based on natural polymer-based hydrogels, demonstrating its potential for the treatment of intrauterine adhesions and regenerative medicine.
✓ 已验证
DOI
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-18
·
IF 14.7
Lu Yichao,Luo Zhenyu,Zhou Huanli,Shi Yingying,Zhu Ying,Guo Xuemeng,Huang Jiaxin,Zhang Junlei,Liu Xu,Wang Sijie,Shan Xinyu,Yin Hang,Du Yongzhong,Li Qingpo,You Jian,Luo Lihua
Obesity often leads to severe medical complications. However, existing FDA-approved medications to combat obesity have limited effectiveness in reducing adiposity and often cause side effects. These medications primarily act on the central nervous system or disrupt fat absorption through the gastrointestinal tract. Adipose tissue enlargement involves adipose hyperplasia and hypertrophy, both of which correlate with increased reactive oxygen species (ROS) and hyperactivated X-box binding protein 1 (XBP1) in (pre)adipocytes. In this study, we demonstrate that KT-NE, a nanoemulsion loaded with the XBP1 inhibitor KIRA6 and α-Tocopherol, simultaneously alleviates aberrant endoplasmic reticulum stress and oxidative stress in (pre)adipocytes. As a result, KT-NE significantly inhibits abnormal adipogenic differentiation, reduces lipid droplet accumulation, restricts lipid droplet transfer, impedes obesity progression, and lowers the risk of obesity-associated non-alcoholic fatty liver disease in female mice with obesity. Furthermore, diverse administration routes of KT-NE impact its in vivo biodistribution and contribute to localized and/or systemic anti-obesity effectiveness.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 21
·59554-59566
·
IF 5.8
Ma Xiao-Yan,Xie Qing-Qing,Hadiya Ablat,Xamsiya Nurmamat,Zhao Zhi-Xi
Dimethyl arsonic acid, the most common organic arsenic pollutant, is widely present in the environment and seriously threatens the safety of drinking water. Syntheses of magnetite, magnetic bentonite, and magnetic ferrihydrite via hydrothermal methods, and the magnetic composites were examined using XRD, BET, VSM, and SEM. SEM images revealed that many monodispersible pellets were attached to the surface of magnetic bentonite. The magnetic ferrihydrite contained abundant pores and had a rich pore structure, which expanded the specific surface area of the original magnetite. The specific surface areas of the magnetic bentonite and magnetic ferrihydrite were 65.17 and 220.30 m 2 ·g −1 , respectively. The adsorption kinetics and adsorption isotherms of dimethyl arsonic acid on magnetic composites were studied. The adsorption of dimethyl arsonic acid on the magnetic composites conformed to the pseudo-second-order model and Freundlich isothermal adsorption model. By comparing the isotherms of the adsorption of dimethyl arsonic acid by the magnetic composites at pH values of 3, 7, and 11, respectively, it was found that the adsorption of dimethyl arsonic acid was the greatest at neutral pH of 7. The adsorption mechanism was analyzed via zeta potential determination, Fourier transform infrared spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS). The zeta potential results revealed that magnetic bentonite electrostatic activity occurred with dimethyl arsonic acid, and the magnetic ferrihydrite indicated a coordination complex with dimethyl arsonic acid. The XPS results revealed that the Fe–O bonds on the surfaces of the magnetic ferrihydrite had coordination complexation effects on the As-O of the dimethyl arsonic acid.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
·
2023
·
IF 1.2
He Yuntao,Guo Xiaxin,Qiao Luyang,Ye Runping,Zong Shanshan,Cao Xinyi,Cheng Jiankai,Zhou Zhangfeng,Yao Yuangen
Rh complexes are considered as the most promising catalysts for formaldehyde hydroformylation since they can achieve higher yields of glycolaldehyde under mild conditions. Although heterogeneous catalysts are convenient to separate, Rh-based heterogeneous catalysts usually have limited catalytic activity in hydroformylation of formaldehyde. Here, four different supports (Al 2 O 3 , SiO 2 , SBA-15 and HMS), loaded with Rh by impregnation method, have been developed for formaldehyde hydroformylation. The physico-chemical properties of the materials have been investigated by means of complementary techniques. Due to the presence of more oxidized rhodium species and stronger metal support interactions, the silica-based catalysts achieved higher formaldehyde conversion and glycolaldehyde selectivity than Rh/Al 2 O 3 . Moreover, SBA-15 and HMS zeolites were synthesized by adjusting the structure of the silica support. Thus, the selectivity of 94.8% for glycolaldehyde and a remarkable glycolaldehyde yield of 52.7% over Rh/SBA-15 catalyst was obtained due to its larger surface area, suitable pore structures and higher dispersion of Rh species.
✓ 已验证
产品关联
DOI
高影响因子
Biomass Conversion and Biorefinery
·
2023
·
IF 4
Lu Yanyu,Lin Changqu,Yang Hao,Ma Peipei,Chen Jishuang,He Zhuang,Wu Hongli,Cao Fei,Chen Kequan,Ouyang Pingkai
Abstract FDCA (2,5-furandicarboxylic acid), produced by oxidizing carbohydrate-derived HMF (5-hydroxymethylfurfural), is a valuable monomer for biopolymers. The difference in the solvent system between the dehydration reaction and oxidation reactions seriously affects the process continuity and production efficiency. Herein, we reported a cascade process for FDCA production from carbohydrates in a single acetic acid system. The fructose conversion was complete and total furan compounds yield reached nearly 90%, which contained 42.8% HMF and 47.1% 5-acetoxymethylfurfural (AMF) in the acetic acid system with NaBr addition. After the azeotropic distillation and vacuum distillation in situ, AMF, a more stable intermediate than HMF, could be easily collected with 90.5% recovery and 99.9% purity. AMF was also oxidized into FDCA with 91.1% yield by Co/Mn/Br catalysts in the acetic acid system. Finally, the cascade process, including fructose dehydration–esterification and HMF/AMF mixture oxidation, was successfully realized in a single acetic acid system. This process is simple to operate, easy to implement, eliminates the use of extra acid or self-made catalysts and expensive solvents, and has the potential for continuous preparation from carbohydrates to FDCA. Graphical Abstract
✓ 已验证
产品关联
DOI
高影响因子
Chinese Journal of Integrative Medicine
·
2023
·
IF 2.9
Gu Jiao-jiao,Wei Ya-ru,Ma Ku,Wang Xiao-qi,Gao Huai-lin
Objective To investigate the effects of Tongxinluo (TXL) on thromboangiitis obliterans (TAO) and the underlying mechanisms. Methods Ninety male C57/BL6J mice were randomly divided into 6 groups according to a random number table: the sham group, TAO model group, Compound Danshen Tablet (CDT) group, and the high-, medium-, and low-dose TXL groups. All mice except the sham group were injected with sodium laurate (0.1 mL, 5 mg/mL) in the femoral artery to establish TAO mouse model. After modeling, mice in the sham and TAO model groups were intragastrically administered 0.5% (w/v) sodium carboxymethylcellulose, mice in the CDT group were intragastrically administered 0.52 g/kg CDT, and mice in the TXL-H, TXL-M, and TXL-L groups were intragastrically administered 1.5, 0.75, and 0.38 g/kg TXL, respectively. After 4 weeks of gavage, the recovery of blood flow in the lower limbs of mice was detected by Laser Doppler Imaging. The pathological changes and thrombosis of the femoral artery were observed by morphological examination. The expressions of tumor necrosis factor α (TNF-α) and inducible nitric oxide synthase (iNOS) in the femoral artery wall were detected by HE staining. Levels of thromboxane B2 (TXB2), 6-keto-prostaglandin F1α (6-keto-PGF1α), endothelin-1 (ET-1), interleukin (IL)-1β and IL-6 were measured using enzyme-linked immunosorbent assay (ELISA). Levels of activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and fibrinogen (FIB) were detected by a fully automated biochemical analyzer. Results TXL promoted the restoration of blood flow in the lower limbs, reduced the area of thrombosis in the femoral artery, and alleviated the pathological changes in the femoral artery wall. Moreover, the levels of TXB2, ET-1, IL-6, IL-1β, TNF-α and iNOS were significantly lower in the TXL groups compared with the model group ( P <0.05 or P <0.01), while the level of 6-keto-PGF1α was significantly higher ( P <0.01). In addition, APTT, PT, and TT were significantly prolonged in TXL groups compared with the model group ( P <0.05 or P <0.01), and FIB levels were significantly decreased compared with the model group ( P <0.01). Conclusions TXL had a protective effect on TAO mice, and the mechanism may involve inhibition of thrombosis and inflammatory responses. TXL may be a potential drug for the treatment of TAO.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）