JOURNAL OF AOAC INTERNATIONAL
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2021
·Vol. 104
·期 6
·1533-1538
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IF 1.913
Shi Qile,Wang Shuya,Zhou Yuxuan,Xu Jingjing
BackgroundBoth deficient and excessive intake of fluoride can lead to adverse health problems.ObjectiveThe aim of this study is to investigate the fluoride content in three types of drinking water in Suzhou urban area.MethodIon chromatography was employed to conduct the validation of analysis of fluoride ion in water and the method was applied to analyze the concentration of fluoride in 22 drinking water samples, including tap water, purified water, and commercially available bottled water, collected from five municipal districts of Suzhou urban area.ResultsThe method was validated in the range of 0.05–2.00 mg/L with good repeatability and accuracy. Results of water analysis indicated that fluoride content in tap water ranged from 0.267 to 0.336 mg/L (average 0.304 mg/L), and the levels of fluoride in purified water and bottled water were 0.068–0.317 mg/L (average 0.134 mg/L) and 0–0.120 mg/L (average 0.080 mg/L), respectively.ConclusionsThe amount of fluoride in all samples analyzed were lower than the limit set for fluoride in drinking water according to the China standard (1.0 mg/L). Low fluoride exposure for the population in the studied area was observed, and the replacement of tap water with purified and bottled water could further aggravate the deficiency of fluoride intake for local residents.HighlightsThe present study is the first to characterize the fluoride content in these three types of drinking water in Suzhou urban area by ion chromatography.
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Frontiers in Physiology
·
2021
·
IF 4.566
Zhang Xiaofang,Liu Panjing,Qin Qiuju,Li Min,Meng Runjie,Zhang Tao
An accurate olfactory system for recognizing semiochemicals and environmental chemical signals plays crucial roles in survival and reproduction of insects. Among all olfaction-related proteins, olfactory receptors (ORs) contribute to the conversion of chemical stimuli to electric signals and thereby are vital in odorant recognition. Olfactory receptor co-receptor (Orco), one of the most conserved ORs, is extremely essential in recognizing odorants through forming a ligand-gated ion channel complex with conventional ligand-binding odorant receptors. We have previously identified aggregation pheromone in Protaetia brevitarsis (Coleoptera: Scarabaeidae), a native agricultural and horticultural pest in East-Asia. However, to our best knowledge, its olfaction recognition mechanisms are still veiled. To illustrate how P. brevitarsis recognize aggregation pheromone and host plants, in the present study we cloned and sequenced the full-length Orco gene from P. brevitarsis antennae (named PbreOrco) and found that PbreOrco is highly conserved and similar to Orcos from other Coleoptera insects. Our real-time quantitative PCR (qRT-PCR) results showed that PbreOrco is mainly expressed in antenna. We also demonstrated that silencing PbreOrco using RNA interference through injecting dsOrco fragment significantly inhibited PbreOrco expression in comparison with injecting control dsGFP and subsequently revealed using electroantennogram and behavioral bioassays that decreasing PbreOrco transcript abundance significantly impaired the responses of P. brevitarsis to intraspecific aggregation pheromone and prolonged the time of P. brevitarsis spending on food seeking. Overall, our results demonstrated that PbreOrco is crucial in mediating odorant perception in P. brevitarsis.
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ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 24
·2100969
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IF 18.808
Juan Li,Jianglong Kong,Sihan Ma,Jiacheng Li,Meiru Mao,Kui Chen,Ziteng Chen,Jiaxin Zhang,Yanan Chang,Hui Yuan,Tong Liu,Zizhu Zhang,Gengmei Xing
Novel boron-containing carbon dots (BCDs) are designed and prepared for boron neutron capture therapy (BNCT) with superior water solubility and excellent optical property for tracking 10 B in vitro and in vivo. Encapsulation of BCDs using exosomes (Exos) from macrophages yields BCD–Exos of ≈100 nm. Fluorescent imaging shows that the BCD–Exos are internalized and distributed around the nuclei of U-87-MG glioma cells. BCD–Exos are also verified to cross the blood–brain barrier and significant accumulation in tumor tissue of the orthotopic U-87-MG glioma tumor-bearing mice model 4 h after administration. Using inductively coupled plasma mass spectrometry (ICP-MS), it is detected that 10 B in tumor tissue is 107.07 ± 1.58 ppm, and T/N ratios are enhanced from 2.03 ± 0.08 of boron phenylalanine (BPA) to 5.28 ± 0.29 of the BCD–Exos. In BNCT, the neutron radiation dose is 8.40 ± 0.12 Gy when a 500 mg kg −1 10 B dosage is given. Finally, there is a prominent BNCT effect of the BCD–Exos-treated brain glioma in the mice model, and the survival ratio is 100% at the end of the experiment. The excellent curative effect of BNCT using BCD–Exos to brain glioma is achieved through adjusted biodistribution of boron in the cancer cell, enhanced T/N ratios, and the precise match between boron and neutron exposure in the site of the tumor in vivo with fluorescence imaging of BCD–Exos.
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Food & Function
·
2021
·Vol. 12
·期 12
·5551-5562
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IF 5.396
Mengjie Ma,Han Wang,Yongkai Yuan,Yuanhong Wang,Shuang Yang,Zhihua Lv
The purpose of this study was to investigate the absorption and transport of glycosaminoglycan from Apostichopus japonicus (AHG) and its depolymerized derivatives (DAHG-1, DAHG-2, DAHG-3). The AHG and depolymerized AHGs (DAHGs) were characterized by high-performance gel permeation chromatography (HPGPC), Raman spectroscopy and atomic force microscopy (AFM). The results showed that there was no significant difference of an AHG primary structure and functional groups during the depolymerization. Meanwhile, AFM observation showed that AHG and DAHGs possessed linear structures. In this study, a rapid and sensitive liquid chromatographic post-column derivatization method was used to investigate the absorption of AHG and DAHGs with the Caco-2 cell model, the M cell model and the intestinal recirculating perfusion. It was found that AHG and DAHGs can be absorbed in the intestine, and their transport involved endocytosis.
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ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 19
·2011073
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IF 18.808
Huiying Chen,Linlin Zhou,Zhi Fang,Shuize Wang,Tao Yang,Laipan Zhu,Xinmei Hou,Hailong Wang,Zhong Lin Wang
Inorganic lead halide perovskite has become an emerging material for modern photoelectric and electronic nanodevices due to its excellent optical and electronic properties. In view of its huge dielectric and electrical properties, inorganic CsPbBr 3 perovskite is introduced into the piezoelectric nanogenerator (PENG). Based on one-step electrospinning of solutions containing CsPbBr 3 precursors and polyvinylidene difluoride (PVDF), in situ growth of CsPbBr 3 nanocrystals in PVDF fibers (CsPbBr 3 @PVDF composite fibers) with highly uniform size and spatial distribution are synthesized. The CsPbBr 3 @PVDF composite fibers based PENG reveals an open-circuit voltage ( V oc ) of 103 V and a density of short-circuit current ( I sc ) of 170 µ A cm −2 , where the V oc is comparable to the state-of-the-art hybrid composite piezoelectric nanogenerators (PENGs) and the density of I sc is 4.86 times higher than that of lead halide perovskites counterpart ever reported. Moreover, CsPbBr 3 @PVDF composite fibers based PENG exhibits fundamentally improved thermal/water/acid–base stabilities. This study suggests that the CsPbBr 3 @PVDF composite fiber is a good candidate for fabricating high-performance PENGs, promising application potentials in mechanical energy harvesting and motion sensing technologies.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xiaoxue Liu,Jiao Yang,Jing Cheng,Yi Xu,Wen Chen,Yingchun Li
Development of nanomaterials with multienzyme-like activities (nanozymes) via a simple way has attracted paramounting attention. Generally, enzymatic activity is closely related to the size, surface state, material type, etc. Bimetallic oxides are very appealing owing to their mixed valence and structural advantages. Herein, we reported an eco-friendly strategy to synthesize Co 1.5 Mn 1.5 O 4 nanozyme via sol-gel method. This nanozyme functionally mimics four major enzymes, i.e. laccase, peroxidase (POD), oxidase (OXD), and catalase (CAT). Accordingly, a colorimetric sensing platform was designed to distinguish isomers of organic pollutants ‒catechol (CC) and hydroquinone (HQ) by using Co 1.5 Mn 1.5 O 4 as the sole catalyst. In particular, laccase-like activity of Co 1.5 Mn 1.5 O 4 enables it to directly oxidize CC into yellow o -benzoquinone with good linearity in the range of 1∼1000 μM and a detection limit of 0.35 μM. Meanwhile, HQ can discolor the oxidized substrate that was pre-oxidized with the aid of oxidase-like activity of the nanozyme (linear range: 0.05∼100 μM, detection limit: 0.04 μM). The established colorimetric method has been successfully utilized for selective sensing of CC, HQ in water samples with good selectivity and high sensitivity. Our study contributes to green synthesis of metal-based nanomaterials with multi-enzyme mimicking activities and uncovers new usage of nanozyme in discriminating of environmental pollutants.
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TOXICOLOGY LETTERS
·
2021
·
IF 4.374
Zhe Wang,Xiaoyu Wang,Zhen Wang,Yaqin Jia,Yuyi Feng,Lili Jiang,Yangliu Xia,Jun Cao,Yong Liu
Osimertinib is the only third-generation epidermal growth factor receptor tyrosine-kinase inhibitor (EGFR-TKI) approved by Food and Drug Administration (FDA). This study aimed to know the inhibitory effect of osimertinib on human UDP-glucosyltransferases (UGTs) and human liver microsomes (HLMs), as well as to identify its potential to cause drug-drug interaction (DDI) arising from the modulation of UGT activity. High inhibitory effect of osimertinib was shown towards UGT1A1, 1A3, 1A6, 1A7, 1A8, 1A10, 2B7 and 2B15. Especially, osimertinib exhibited competitive inhibition against UGT1A1 with a K i,u of 0.87 ± 0.12 μM. It also noncompetitively inhibited SN-38 glucuronidation in pooled HLMs with a K i,u of 3.32 ± 0.25 μM. Results from quantitative prediction study indicated that osimertinib administered at 80 mg/day may result in a 4.83 % increase in the AUC of drugs mainly metabolized by UGT1A1, implying low risk of DDI via liver metabolism. However, the ratios of [ I ] gut / K i,u are much higher than 11 in HLMs and recombinant UGT1A1, indicating a risk for interaction in intestine. The effects of osimertinib on intestinal UGT should be paid more attention on to avoid unnecessary clinical DDI risks.
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Bioactive Materials
·
2021
·
IF 14.593
Yamin Chai,Zhuang Liu,Yunzheng Du,Lichun Wang,Jinyan Lu,Qian Zhang,Wenyan Han,Tingting Wang,Yameng Yu,Lisha Sun,Lailiang Ou
Highly efficient removal of bilirubin from whole blood directly by hemoperfusion for liver failure therapy remains a challenge in the clinical field due to the low adsorption capacity, poor mechanical strength and low biocompatibility of adsorbents. In this work, a new class of nanocomposite adsorbents was constructed through an inorganic-organic co-crosslinked nanocomposite network between vinyltriethoxysilane (VTES)-functionalized hydroxyapatite nanoparticles (V-Hap) and non-ionic styrene-divinylbenzene (PS-DVB) resins (PS-DVB/V-Hap) using suspension polymerization. Notably, our adsorbent demonstrated substantially improved mechanical performance compared to the pure polymer, with the hardness and modulus increasing by nearly 3 and 2.5 times, respectively. Moreover, due to the development of a mesoporous structure, the prepared PS-DVB/V-Hap3 exhibited an ideal adsorption capacity of 40.27 mg g −1 . More importantly, the obtained adsorbent beads showed outstanding blood compatibility and biocompatibility. Furthermore, in vivo extracorporeal hemoperfusion verified the efficacy and biosafety of the adsorbent for directly removing bilirubin from whole blood in pig models, and this material could potentially prevent liver damage and improve clinical outcomes. Taken together, the results suggest that PS-DVB/V-Hap3 beads can be used in commercial adsorption columns to threat hyperbilirubinemia patients through hemoperfusion, thus replacing the existing techniques where plasma separation is initially required.
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ACS Catalysis
·
2021
·
IF 13.084
Lipeng Wu,Bin Li,Yang Li,Xiaobin Fan,Fengbao Zhang,Guoliang Zhang,Qing Xia,Wenchao Peng
Herein, a series of Co@N–C materials are synthesized using nitrates (LiNO3, NaNO3, and KNO3) as templates to control the preferential growth of the cobalt (200) facet. Co@N–C–X (X = LiNO3, NaNO3, and KNO3) exhibit excellent activities and long-term stabilities for Fenton-like degradation of bisphenol A (BPA) with peroxymonosulfate (PMS) as an oxidant. The texture coefficient (TC) is used to investigate the relationship between the Co facets and their catalytic activities, and a linear relation is built between the TC of Co(200) and the reaction rate constant (k). Co(200) is therefore the most active facet for PMS activation, while nitrates can promote the preferential growth of the Co(200) facet. Moreover, the Co(200) facet is more stable against leaching and can achieve more quick transformation between Co2+/Co3+. Quenching tests, electron paramagnetic resonance spectra, and electrochemical tests show that singlet oxygen (1O2) plays the dominant role for BPA degradation. Density functional theory verifies that Co nanoparticles coated with a graphitic nitrogen-doped carbon layer have the best activity for PMS activation. This work first proposes the strategy of using nitrates to control the preferential growth of Co facets and reveals the relationship between the TC value and k, thus attracting great interest in the environmental field.
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JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Xiaofang Niu,Han Liu,Su Xue,Jinli Zhang,Wei Li
The solubility data of flonicamid (FA) in twelve mono-solvents (methanol (MA), ethanol (EA), n-propanol (NPA), isopropanol (IPA), n-butanol (NBA), isobutanol (IBA), ethyl acetate (EAC), butyl acetate (BAC), acetone (AT), acetonitrile (AN), tetrahydrofuran (THF), 1,4-dioxane (DX)) and a binary solvent of EA + EAC were experimentally measured by using a gravimetric method under atmospheric pressure. The tested temperature range for the studied solvents was from 283.15 K to 323.15 K except of 288.15–323.15 K for DX. Powder X-ray diffraction (PXRD) was applied to evaluate the crystal form of FA, and no solvate or new form formation were observed during the whole experiment. The experimental results show that the rising temperature leads to the increase of the FA solubility in all the studied mono and mixed solvents at the tested ranges. For mono-solvents, the solubility of FA at 293.15 K increases in the order: IBA (0.002641) < IPA (0.004243) < NPA (0.004361) < NBA (0.004750) < BAC (0.006844) < EA (0.006864) < EAC (0.01342) < MA (0.01394) < DX (0.01747) < AN (0.02455) < THF (0.03433) < AT (0.04645). For EA + EAC mixed solvents, the solubility of FA increases and then decreases with the rising mole fraction of EAC. That means addition of EAC to EA can alter the FA solubility. Density functional theory (DFT) calculations were performed to analyze the interactive energy between FA and the solvent molecules. Furthermore, the Apelblat equation, van’t Hoff equation, λh equation are applied to correlate the experimental solubility data of FA in mono and mixed solvent systems, while NRTL model and Wilson model are selected for mono-solvents, CNIBS/R-K model and Jouyban-Acree model for mixed solvents. Correlated results demonstrate that the Apelblat model provides the best regression. In addition, the dissolution thermodynamic properties of FA in chosen solvents are analyzed by the van’t Hoff equation. It suggests that the dissolution processes of FA are non-spontaneous, endothermic and entropy increase in these studied systems.
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JOURNAL OF FOOD SCIENCE
·
2021
·Vol. 86
·期 5
·2118-2130
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IF 3.167
Lingyu Zhu,Yang Song,Huilin Liu,Min Wu,Haizhou Gong,Hainan Lan,Xin Zheng
β-Carotene displays antioxidant and anti-inflammatory activities and prevents the development of cancer. Ulcerative colitis (UC) is a kind of inflammatory bowel disease that is accompanied by a certain risk of colon cancer. However, the role of β-carotene in the modulation of gut microbiota and UC improvement is unclear. In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS). The results revealed that β-carotene significantly ( p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged. Moreover, colonic levels of proinflammatory cytokines were significantly lower following β-carotene supplementation. β-Carotene intervention also lowered the expression levels of phosphorylated p65 (0.60 ± 0.02), p38 (0.57 ± 0.00), Erk (0.63 ± 0.04), and JNK (0.70 ± 0.00). The result of the relative abundance of gut microbiota showed that DSS administration significantly changed the microbial structure at the phylum and genus levels of rats. Furthermore, β-carotene treatment significantly increased the abundance of Faecalibacterium , the levels of which negatively correlated with the levels of inflammatory cytokines. Faecalibacterium may be a potential target in the alleviation of DSS-induced UC. β-Carotene can alleviate DSS-induced UC through the regulation of gut microbiota. This study provides a reference for the rational use of β-carotene in the treatment of UC. Practical Application β-Carotene can relieve ulcerative colitis and regulate the gut microbiota; the nutritional intervention of β-carotene enhancing animal health.
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Journal of Magnesium and Alloys
·
2021
·
IF 10.088
Baojing Feng,Guonan Liu,Peixu Yang,Sensen Huang,Dongqing Qi,Peng Chen,Chengduo Wang,Jiang Du,Shaojun Zhang,Jinhui Liu
Effect of the second phase in the micro-galvanic corrosion of a commercial Mg alloy containing rare earth elements, cast WE43 alloy, was investigated in 0.6 M NaCl solution and 0.6 M Na 2 SO 4 solution by scanning electron microscopy (SEM) observations, scanning Kelvin probe force microscopy (SKPFM) analysis, hydrogen evolution, weight loss measurement, and electrochemical techniques. It is confirmed that the second phase of cast WE43 alloy is more active than Mg matrix and exhibits an anodic role in the micro-galvanic corrosion with α-Mg matrix as cathode and dissolves preferentially in Na 2 SO 4 solution, in contrast to the situation in NaCl solution. The corrosion rate of cast WE43 alloy in Na 2 SO 4 solution is much higher than that in NaCl solution, which is different from the conventional wisdom and could be attributed to the different role of the second phase in the micro-galvanic corrosion in two solutions.
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危险品化学品经营许可证（带存储）