INTERNATIONAL ORTHOPAEDICS
·
2010
·Vol. 35
·期 10
·1561-1567
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IF 1.825
Li Hong,Ge Yunsheng,Wu Yang,Jiang Jia,Gao Kai,Zhang Pengyun,Wu Lingxiang,Chen Shiyi
The purpose of this study was to investigate whether hydroxyapatite (HAp) coating could induce polyethylene terephthalate (PET) artificial ligament graft osseointegration in the bone tunnel. Twenty-four New Zealand white rabbits underwent artificial ligament graft transplantation in bilateral proximal tibia tunnels. One limb was implanted with HAp-coated PET graft, and the contralateral limb was implanted with non-HAp-coated PET graft as control. The rabbits were randomly sacrificed at four and eight weeks after surgery. The loads to failure of the experimental group at eight weeks were significantly higher than those of the control group ( p = 0.0057). Histologically, application of HAp coating induced new bone formation between graft and bone at eight weeks compared with the controls. Real-time polymerase chain reaction examination revealed significantly elevated messenger ribonucleic acid expression levels of osteopontin and collagen I in the grafts of the HAp group compared with the controls at four weeks ( p < 0.05). The study has shown that HAp coating on the PET artificial ligament surface has a positive effect in the induction of artificial ligament osseointegration within the bone tunnel.
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BMC BIOTECHNOLOGY
·
2019
·Vol. 19
·期 1
·1-11
·
IF 2.303
Zhang Ge,Li Shugui,Xu Yingbo,Wang Juan,Wang Fan,Xin Yuhua,Shen Zhong,Zhang Haibo,Ma Ming,Liu Haobao
Background Tobacco stalk (TS), a major agricultural waste abundant in pectin, has resulted in concerns about the need for its reuse. The nicotine in TS is considered a chemical that is to\xic and hazardous to the environment. Results In this study, Bacillus tequilensis CAS-MEI-2-33 was isolated from cigar wrappers to produce alkaline pectinase using TS. Subsequently, the medium and fermentation conditions for the production of pectinase by B . tequilensis CAS-MEI-2-33 were optimized. The optimal fermentation period, pH of the initial fermentation medium, concentration of TS, and inoculum amount for B . tequilensis CAS-MEI-2-33 were 40 h, 40 g/L, 7.0, and 3%, respectively. Under optimal conditions, the pectinase activity was 1370 U/mL. Then, the enzymatic properties, such as the optimum pH, reaction temperature, temperature stability, and effects of metal ions, were studied. The optimal pH was determined to be 10.0, indicating that the enzyme was an alkaline pectinase. The optimal temperature was 40 °C, and pectinase activity was stable at 40 °C. The Ag + metal ions were shown to remarkably promote enzyme activity. The pectinase was partly purified by ammonium sulfate precipitation, ion exchange chromatography, and Sephacryl S-100 chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and LC-MS/MS analyses were utilized to analyze the pectinase. Conclusions This study provided a new alkaline pectinase candidate and a new strategy for the use of TS.
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3 Biotech
·
2017
·Vol. 8
·期 1
·1-8
·
IF 1.361
Yuan Shengliang,Duan Zhihong,Lu Yingnian,Ma Xiaoli,Wang Sheng
Gracilaria lemaneiformis is mainly used as a raw material source for agar industry, and its extract is rich in natural pigments with antioxidant activities. In this study, a solvent reflux extraction method for decolorization of G. lemaneiformis has been developed in agar production. The extraction conditions were optimized as follows: solvent-to-material ratio, 50:1; ethanol concentration, 70%; number of extractions, 3; extraction time, 0.5 h, under which the total antioxidant yield of the extract reached 2.89 ± 0.88 mg/g dried seaweeds. Their IC 50 values of DPPH radical scavenging activity, hydroxyl radical scavenging activity and superoxide anion scavenging activity were 21.91 ± 1.8 mg/L, 40.59 ± 1.5 mg/L and 160.87 ± 2.8 mg/L, respectively. Further isolation and spectroscopic analysis of natural pigments suggested the strong antioxidant capacities were attributed to chlorophyll derivatives. The results indicate that the decolorization process was able to improve the agar quality, and the extract containing lots of natural pigments had antioxidant activities which may be used in functional food and cosmetics.
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AAPS PHARMSCITECH
·
2012
·Vol. 13
·期 2
·485-492
·
IF 1.432
Shi Jun,Wang Yiming,Luo Guoan
In the present study, we have investigated transdermal administration of ligustrazine phosphate (LP), as an antioxidant, for the treatment of Alzheimer’s disease (AD). The LP transdermal ethosomal system was designed and characterized. Franz-type diffusion cells and confocal laser scanning microscopy were used for the in vitro permeation studies. Furthermore, the effect of LP transdermal ethosomal system on AD was evaluated in the scopolamine-induced amnesia rats by evaluating the behavioral performance in the Morris water maze test. The activities of the antioxidant enzymes and the levels of the lipid peroxidation product malondialdehyde (MDA) in the brain of rats were also determined. The results showed that both the penetration ability and the drug deposition in skin of the LP ethosomal system were significantly higher than the aqueous one. The LP transdermal ethosomal system could recover the activities of the antioxidant enzymes and the levels of MDA in the brain of the amnesic rats to the similar status of the normal rats, which was also indirectly reflected by the improvement in the behavioral performance. In conclusion, LP might offer a potential alternative therapeutic drug in the fight against AD, and ethosomes could be vesicles of choice for transdermal delivery of LP.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 42
·62994-63004
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IF 5.19
Lu Yuanyuan,Liu Xuna,Miao Yanjun,Chatzisymeon Efthalia,Pang Lina,Qi Luqing,Yang Ping,Lu Hongyan
Roles of bulk-, micron-, and nano-copper oxide (CuO) on methane production, microbial diversity, functions during thermophilic anaerobic digestion (AD) were investigated in this study. Results showed that bulk-, micron-, and nano-CuO promoted methane production by 27.8%, 47.6%. and 83.1% compared to the control group, respectively. Microbial community analysis demonstrated that different particle sizes could cause various shifts on bacteria community, while had little effect on archaeal diversity. Thereinto, bacteria belonging to phylum Firmicutes and Coprothermobacterota dominated in enhanced hydrolysis process in groups with nano-CuO and bulk-CuO, respectively, while micron-CuO had stronger promotion on the abundances of hydrolytic and fermentative bacteria belonging to families Peptostreptococcaceae, Caloramatoraceae, Erysipelotrichaceae, and Clostridiaceae, than other two CuO sizes. Metabolic pathways revealed that energy-related metabolism and material transformation in bacteria were only boosted by micron-CuO, and nano-CuO and bulk-CuO were important to methanogenic activity, stimulating energy consumption and methane metabolism, respectively.
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Cell Death Discovery
·
2025
·Vol. 11
·期 1
·555
·
IF 7
Deng Zhilin,Huang Shuying,Pei Yu,Li Hanxiang,Dong Yong,Li Yuanyuan,Ran Qin,Liu Xindong,Feng Yi,Wang Qiang,Guo Zhenhua,Huang Sizhou
The proliferation of early erythrocyte and the subsequent maturation are critical events during erythropoiesis, while how these two independent but interconnected processes are efficiently orchestrated during erythropoiesis is largely unknown. Prpf4 expression is enriched from Pre-Colony Forming Unit-Erythroid (PreCFU-E) to Nucleated Erythrocytes, especially in the CFU-E cells, implying that Prpf4 plays a critical role in erythropoiesis. Here, we demonstrate that prpf4 sequentially regulates erythrocyte proliferation and maturation during zebrafish definitive hematopoiesis. The data show that prpf4 mutation results in severe defects in erythropoiesis, characterized by a substantial reduction in erythroid cell numbers and impaired erythrocyte maturation. Further analysis indicates that prpf4 mutation leads to cell cycle arrest of erythrocytes at the S and G2/M phases, as well as a significant increase in erythrocyte apoptosis. Mechanistically, prpf4 mutation leads to DNA damage and the subsequent activation of the DNA damage response, triggering the ATM/CHK2-p53 signaling pathway. This process inhibits the proliferation of early erythrocyte and induces erythrocyte apoptosis. On the other hand, the data reveal that prpf4 mutation causes significant defects in skipped-exon during pre-mRNA splicing, accompanied by severe splicing defect in slc25a39 pre-mRNA. This results in a significant downregulation of slc25a39 mRNA, which partially impairs erythrocyte maturation during late erythropoiesis. In conclusion, we identify that prpf4 sequentially regulates early erythrocyte proliferation and subsequent erythrocyte maturation. This dual function of prpf4 partially explains how early erythrocyte proliferation and late maturation are efficiently coordinated during erythropoiesis.
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Nature Communications
·
2025
·
IF 15.7
Yang Wan,Pan Tengfei,Yang Guang,Zhu Jianbing,Ran Xueqin,Ren Biyun,Zuo Mengnan,Chao Lingfeng,Xia Yingdong,Chen Yonghua
Interfacial dipolar molecules play a crucial role in achieving high-performance perovskite solar cells (PSCs). However, the random distribution at the interface often limits their ability to effectively regulate interfacial energy levels and carrier extraction. Here, we report a bifunctional antisymmetric molecule, N-(1-carboxyethyl)iminodiacetic acid trisodium salt (MGDA·3Na), which enables both directional alignment and stable anchoring on SnO 2 and perovskite interfaces. The methyl side chain in the MGDA - anion induces intramolecular electronic polarization and dipole reorientation, facilitating selective adsorption on the SnO 2 surface and perovskite buried interface. This significantly improves interface uniformity, enabling efficient passivation of SnO 2 surface defects and precise regulation of perovskite crystal growth. The dual anchoring effect not only aligns energy levels to minimize charge extraction barriers but also templates vertical perovskite growth, reducing buried interface defects and enhancing crystallization quality. As a result, we achieved a power conversion efficiency of 26.43% in small-area PSCs and 23.27% in a 5 × 5 cm 2 large-area module, highlighting the potential for industrial-scale application. The device maintains 96% of its initial efficiency for 2000 h under nitrogen-filled glove box, 96% after 800 h at 55 °C/55% RH, and 99% after 800 h of continuous illumination at the maximum power point.
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Journal of Food Measurement and Characterization
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2025
·
IF 3.3
Ma Qiu-Yue,Xu Qian-Da,Chen Nan,Zeng Wei-Cai
Accurate determination of total phenolic content in protein-rich foods remains challenging due to methodological interferences. This study systematically investigated the effects of protein properties (concentration, molecular weight, and isoelectric point) on three common total phenolic content determination methods (Folin-Ciocalteu, Ferrous tartrate, and Prussian blue assays). Using bovine serum albumin (BSA, 66 kDa, pI 4.7), casein (23.6 kDa, pI 4.6), and cytochrome C (12.4 kDa, pI 10.2) as model proteins, with gallic acid as the phenolic standard. Results demonstrated that the synergistic effect of protein concentration and molecular weight significantly influenced the measurements. The Folin-Ciocalteu assay exhibited the most pronounced interference from high protein concentrations (≥ 1 mg/mL) and large molecular weights, primarily due to protein-copper chelation and competitive chromogenic reactions involving aromatic amino acids. The Ferrous tartrate method was sensitive to phosphorylated proteins (e.g., casein) owing to competitive Fe 2+ complexation by phosphate groups. In contrast, the Prussian blue method showed superior anti-interference capability (recovery > 92%), though protein precipitation at high concentrations (≥ 2 mg/mL) could lead to underestimation. A multiple linear regression model was established to quantify the relationship between protein characteristics and interference intensity. These findings provide quantitative relationships between protein properties and assay performance, offering practical guidance for selecting appropriate total phenolic content methods in complex food matrices. The study highlights the need for protein-specific method optimization to ensure accurate phenolic quantification in nutritional and functional food analysis.
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Communications Chemistry
·
2025
·
IF 6.2
Xu Xiaoli,Wang Ying,Cheng Wanwan,Zhai Huan,Xiao Lingbo,Qin Laishun,Khanbareh Hamideh,Bowen Chris,Jia Yanmin,Wen Zhenhai,Chen Da
Piezoelectric materials with high piezoelectric coefficients often exhibit low carrier concentrations, which hinders the generation of free charges for piezocatalytic processes. To address this, defect engineering has emerged as a promising approach to adjust the electronic structure and enhance the carrier concentration of piezocatalytic materials. However, the introduction of defects can negatively affect the piezoelectric performance by increasing charge screening and reducing domain wall mobility. In this work, we demonstrate that a high carrier concentration can be achieved without compromising the piezoelectric properties, by applying an external electric field to enhance polarization and rearrange oxygen defect dipoles. This poling process significantly improves both piezoelectric and piezocatalytic properties. Specifically, the effective piezoelectric coefficient of a polarised, oxygen-deficient Bi 2 WO 6- x material increased from d * 33 ~ 4.79 pm V ‒1 to d * 33 ~ 39.89 pm V ‒1 . Moreover, its carrier concentration remained high, approaching N d ~ 7.39×10 20 cm ‒3 , when subjected to ultrasonic vibrations. As a result of the high piezoelectric activity and carrier concentration, the piezocatalytic hydrogen production efficiency (per unit power) attained ~3.49 μmol g −1 h −1 W −1 . This work enables the design of efficient and energy-saving piezocatalytic materials, providing a significant step forward for hydrogen production via piezocatalytic water splitting.
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JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
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2025
·
IF 2
Zhang Ling,Zhao Ronglin,Dong Yaqiang,Jia Xingjie,Zhang Bowei,He Aina,Li Jiawei,Shen Baogen
In the present work, the thermal stability, insulation performance, and magnetic properties of nanocrystalline soft magnetic powder cores (NSMCs) have been greatly improved, thanks to the successful creation of composite materials that combine inorganic nano-Cr 2 O 3 with organic epoxy resins. By coating the nano-Cr 2 O 3 /epoxy insulating layer on the FeSiBNbCu nanocrystalline powders, the core loss ( P cv ) of the FeSiBNbCu NSMCs is successfully decreased and the DC-bias performance is significantly enhanced. When the content of nano-Cr 2 O 3 is 0.3 wt.%, the FeSiBNbCu NSMCs demonstrate optimal comprehensive soft magnetic properties, including low P cv of 106.2 mW/cm 3 at 100 kHz/50 mT, high effective permeability ( μ e ) of 53.6 at the frequency of 1 MHz and DC-bias characteristics of 62% at a bias field of 100 Oe. As the coating layer of the NSMCs, the nano-Cr 2 O 3 /epoxy composite material has a straightforward, practical, and affordable production process. It is also more suited for industrialization and has a wide range of potential industrial applications.
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Nature Communications
·
2025
·
IF 15.7
Yi Li,Chen Chaohao,Wen Yingjie,Zhang Sixie,Chen Haocheng,Zhu Jinchao,Weng Jiabin,Zhang Wuyong,Xu Wenwen,Guan Wanbing,Chen Xu,Qiu Tianyu,Tian Xinlong,Lu Zhiyi
The electrochemical synthesis of solid compounds is a critical emerging area in electrocatalysis; however, a major challenge is still remaining on severe catalysts scaling driven by strong solid-solid interactions, giving rise to rapid electrode deactivation. In this study, for the example of simultaneous synthesis of Mg(OH) 2 and H 2 from natural seawater electrolysis, we demonstrate that Pt catalyst with surface-coordinated halogens (F, Cl, Br, I) can alleviate this scaling effect (i.e., solidophobicity) by like-charge repulsion and thus regulating the local environment. Specifically, Pt-I coordination results in supersolidophobicity, achieving a successful extraction of Mg(OH) 2 ( > 99% purity) while stably producing H 2 (under 100 mA cm -2 for over 5000 hours). A combination of experimental and theoretical studies reveals that, due to the like charge repulsion between I - and in situ generated OH - , the Pt-I catalyst regulates the surface pH gradient which increases the distance from the electrode surface to the Mg(OH) 2 nucleus (>4 μm) and facilitates homogeneous nucleation. Additionally, the scalability of the Pt-I catalyst, along with the techno-economic analysis and life cycle assessment of the natural seawater electrolysis technology are systematically demonstrated.
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Nature Communications
·
2025
·
IF 15.7
Xiong Xuhui,Liu Zhengwang,Zhang Ruixuan,Yang Liting,Liang Guisheng,Pei Ke,Che Renchao
Commercial negative electrodes such as graphite and Li 4 Ti 5 O 12 are fundamental to lithium-ion batteries but face inherent trade-offs among safety, energy density, rate performance, and cycling stability. In this work, we introduce structural ordering and vacancy engineering into a perovskite negative electrode Ce 2/3 TiO 3 to tackle this dilemma, by creating highly ordered Ce vacancies that form a stable superlattice. As a result, micron-sized Ce 2/3 TiO 3 achieves a high specific capacity (>200 mAh g -1 ) at an optimal operating potential (~0.8 V vs. Li + /Li), with fast-charging capability up to 50 C and stable cycling performance exceeding 10000 cycles at 20 C. Its electrochemical performance has the potential to overcome the shortcomings of graphite and Li 4 Ti 5 O 12 , comparable to many representative intercalation-type negative electrodes. In situ structural analysis and atomic-scale imaging reveal a reversible topological phase transition between long-range and short-range ordering, which preserves the lattice integrity while unlocking low-barrier Li + diffusion pathways. Here, we show that vacancy ordering provides a compelling strategy for designing high-performance electrodes.
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