DYES AND PIGMENTS
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2022
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IF 5.122
Lin Yuan,Lei Jiang,Ze Liu,Yingying Xiang,Fei Song,Yujiao Tu
Tartrazine preferred in food and textile industries, but excessive consumption threats human health. Due to toxicity, non-biodegradability, and trace accumulation in the food chains, Hg 2+ has attracted people's attention. Thus, it is important to detect tartrazine and Hg 2+ with sensitivity way. Using VB 2 and citrate as precursors, yellow-green fluorescent carbon dots were prepared by hydrothermal method . The N-CDs were purified by dialysis, thin layer chromatography and chromatographic column. Excellent linear ranges of 0.25–5 μM and 5–50 μM of tartrazine were obtained with low detection limits 34.98 nM, while good linear ranges of 0.25–5 μM and 10–50 μM of Hg 2+ with low detection limits 29.64 nM, respectively. In addition, the quenching mechanisms of targets to the fluorescent of N-CDs were illuminated. Furthermore, the nanoprobe was efficiently applied to determine the content of tartrazine in carbonated drink, fruit jelly and sugar samples, and Hg 2+ in tap water, lake water, and river water samples. It showed great potential to detect tartrazine and Hg 2+ .
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Drug Design Development and Therapy
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2022
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IF 4.319
Shurong Duan,Fei Wang,Jingwei Cao,Chunyan Wang
Introduction Intracerebral hemorrhage (ICH) is a devastating type of stroke with high mortality, and the effective therapies for ICH remain to be explored. Exosomes (Exos) have been found to play important roles in cell communication by transferring molecules, including microRNAs (miRNAs/miRs). MiRNAs are critical regulators of genes involved in many various biological processes and have been demonstrated to aggravate or alleviate brain damages induced by ICH. The aim of the present study was to investigate the effect of Exos derived from miR-146a-5p-enriched bone marrow mesenchymal stem cells (BMSCs-miR-146a-5p-Exos) on experimental ICH.Methods ICH was induced in adult male Sprague-Dawley rats by an intrastriatal injection of collagenase type IV. At 24 h after surgery, Exos were administrated. For detecting apoptotic cells, TUNEL staining was performed using an in situ Cell Death Detection Kit. Fluoro-Jade B staining was performed to detect degenerating neurons. Immunofluorescence assay was performed to detect the expression of myeloperoxidase (MPO) and OX-42. The binding of miR-146a-5p and its target genes was confirmed by luciferase reporter assay.Results At 24 h after surgery, BMSCs-miR-146a-5p-Exos administration significantly improved neurological function, reduced apoptotic and degenerative neurons, and inhibited inflammatory response. Furthermore, miR-146a-5p-enriched Exos obviously inhibited the M1 polarization of microglia after ICH in rats, accompanied by the reduced expression of pro-inflammatory mediators releasing by M1 microglia including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and monocyte chemoattractant protein-1 (MCP-1). Finally, we observed that miR-146a-5p directly targeted interleukin-1 receptor-associated kinase1 (IRAK1) and nuclear factor of activated T cells 5 (NFAT5), which contributed to the inflammation response and the polarization of M1 microglia/macrophages.Conclusion We demonstrated that miR-146a-5p-riched BMSCs-Exos could offer neuroprotection and functional improvements after ICH through reducing neuronal apoptosis, and inflammation associated with the inhibition of microglial M1 polarization by downregulating the expression of IRAK1 and NFAT5.
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Acta Biomaterialia
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2022
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IF 10.633
Junqin Li,Wenhua Yi,Yuze Luo,Ke Yang,Lidan He,Caiyun Xu,Le Deng,Dinggeng He
Nanozyme-based chemodynamic therapy (CDT) has shown tremendous potential in the treatment of bacterial infections. However, the CDT antibacterial efficacy is severely limited by the catalytic activity of nanozymes or the infection microenvironments such as insufficient hydrogen peroxide (H 2 O 2 ) and overexpressed glutathione (GSH). Herein, a versatile hybrid nanozyme (MoS 2 /CuO 2 ) is rationally constructed by simply decorating ultrasmall CuO 2 nanodots onto lamellar MoS 2 platelets of hydrangea-like MoS 2 nanocarrier via a covalent Cu-S bond. The MoS 2 /CuO 2 nanozyme exhibits the peroxidase-mimic activity for catalytically converting H 2 O 2 produced by acid-triggered decomposition of the decorated CuO 2 into hydroxyl radical (•OH). Meanwhile, the MoS 2 /CuO 2 can consume GSH overexpressed in the infection sites via redox reaction mediated by polyvalent transition metal ions (Cu 2+ and Mo 6+ ) for enhanced CDT. More importantly, MoS 2 support can promote the conversion of Cu 2+ to Cu + by a co-catalytic reaction based on the Mo 4+ /Mo 6+ redox couples, and provide photonic hyperthermia (PTT) to augment the peroxidase-mimic activity. The developed MoS 2 /CuO 2 nanozymes possesses a desirable catalytic property, as well as a remarkably improved antibacterial efficiency both in vitro and in vivo . Taken together, this study proposes a synergetic multiple enhancement strategy to successfully construct the versatile hybrid nanozymes for intensive in vivo PTT/CDT dual-mode anti-infective therapy. Statement of significance Chemodynamic therapy (CDT) has shown great potentialities in the treatment of bacterial infections, while its therapeutic efficiency is severely limited by the infection microenvironments such as insufficient hydrogen peroxide (H 2 O 2 ) and overexpressed glutathione (GSH). Here, we rationally construct a hybrid nanozyme (MoS 2 /CuO 2 ) with peroxidase-like activity that can enhance CDT by regulating local microenvironments, that is, simultaneously self-supplying H 2 O 2 and consuming GSH. Importantly, MoS 2 support can promote the conversion of Cu 2+ to Cu + by the Mo 4+ /Mo 6+ redox couples, and provide photonic hyperthermia (PTT) to augment the peroxidase-mimic activity. The developed MoS 2 /CuO 2 shows desirable PTT/CDT dual-mode antibacterial efficacy both in vitro and in vivo . This study proposes a versatile hybrid nanozyme with multiple enhancement effects for intensive in vivo anti-infective therapy.
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Biomaterials Science
·
2022
·Vol. 10
·期 23
·6791-6803
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IF 7.59
Yandong Xie,Xueying Lu,Zhen Wang,Mingxi Liu,Liang Liu,Ran Wang,Kun Yang,Hong Xiao,Jianyong Li,Xianglong Tang,Hongyi Liu
Glioblastoma (GBM), as the most aggressive adult brain tumor, seriously threatened people's lives with a low survival time. Standard postoperative treatment, chemotherapy combined with radiotherapy (RT), was the major therapeutic strategy for GBM. However, this therapeutic efficacy was hindered by chemoradiotherapy resistance of GBM. Herein, to sensitize temozolomide (TMZ)-based chemotherapy and RT, a hypoxia-radiosensitive nanoparticle for co-delivering TMZ and siMGMT (RDPP(Met)/TMZ/siMGMT) was synthesized in this study. Our nanoparticle could effectively release the encapsulated alkylating agent (TMZ) and small interfering O6-methylguanine-DNA-methyltransferase RNA (siMGMT) in the hypoxic GBM. DNA-damage repair was effectively inhibited by down-regulating MGMT expression and activating cell apoptosis, which obviously enhanced the sensitivity of TMZ as well as RT. In vitro and in vivo experiments showed that RDPP(Met)/TMZ/siMGMT could efficiently penetrate the blood–brain barrier (BBB), accurately target GBM cells and effectively inhibit GBM proliferation. Compared with traditional TMZ combined with RT, RDPP(Met)/TMZ/siMGMT remarkably improved the survival time of orthotopic GBM-bearing mice, which demonstrated that our nanoplatform was an efficient combinatorial GBM therapy.
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ANALYST
·
2022
·Vol. 147
·期 24
·5633-5642
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IF 5.227
Haijiao Dong,Guancheng Wang,Kaizheng Feng,Xiaohan Wu,Yaoyao Fan,Wei Zhang,Ming Ma,Ning Gu,Yu Zhang
Nanozymes with wide applications have rapidly attracted tremendous attention from various fields in the last decade. However, research on the standardization of nanozymes is still lacking. Currently, the accurate evaluation and effective tracing of the enzyme-like activity of nanozymes have become a common concern. This work aims to develop a certified reference material (CRM) of Prussian blue nanozymes (PBNEs) for their peroxidase (POD)-like activity. The homogeneity and stability studies demonstrated that the property value of POD-like activity is consistent across different packing units, and remains unchanged during the one-year validity period of storage in the dark at 4 °C. The certified value of the POD-like activity of the PBNE CRM is assigned as 174 ± 13 U mg−1 (k = 2) by interlaboratory comparison studies and traceable uncertain evaluation. Furthermore, the need for quality control of the POD-like activity of nanozymes was exemplified by comparing the influence of two additional PBNEs on the dry and wet chemical detection of glucose (Glu). As the first quality assurance tool of nanozymes, the PBNE CRM is expected to replace natural horse radish peroxidase (HRP) as an effective benchmark for assessing the analytical method and laboratory competence. In addition, this work also inspires the further standardization of nanozymes.
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CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Yuehua Shi,Yu Shi,Zhaoyang Wang,Jiahe Zhang,Ran Hao,Gangwan Zhang,Xiaolong Zhao,Leyong Zeng
To achieve safe and efficient tumor therapy, it was a powerful strategy of constructing an intelligent tumor microenvironment (TME)-responsive nanoprobe. In this study, by coating ultrasmall gold nanoparticles (AuNPs) and manganese dioxide (MnO 2 ) on mesoporous prussian blue (MPB), [email protected] @MnO 2 nanoprobe with H 2 O 2 self-generation was developed for magnetic resonance (MR) imaging, photothermal therapy (PTT) and enhanced chemodynamic therapy (CDT). The results indicated that the prepared [email protected] @MnO 2 possessed uniform size and good biocompatibility. Using glucose-responsive self-supplying H 2 O 2 of ultrasmall AuNPs, and glutathione (GSH)-responsive Mn 2+ release from MnO 2 degradation, the accelerated hydroxyl free radicals (·OH) generation efficiency (80.0 % to 90.84 %) and enhanced MR relaxation (1.93 to 9.96 mM −1 ·s −1 ) were achieved. Importantly, the prepared [email protected] @MnO 2 exhibited excellent photothermal performance with a photothermal conversion efficiency of 42.62 %. Moreover, the intracellular H 2 O 2 /·OH generation was demonstrated, and the in vitro CDT/PTT was enhanced, by which the cell viability was reduced to 6.4 %, stronger than 18.8 % of [email protected] 2 . By modeling MDA-MB-435 tumor-bearing nude mice, the combined CDT/PTT of [email protected] @MnO 2 nanoprobe greatly inhibited the tumor growth, and after 15 days, the tumor completely disappeared. This work provided a promising strategy to develop TME-responsive self-supplying H 2 O 2 nanoprobe, achieving MR imaging and enhanced antitumor efficacy.
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CHEMOSPHERE
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2022
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IF 8.943
Zhongbao Ma,Long-Fei Ren,Diwen Ying,Jinping Jia,Jiahui Shao
The separation of hardness ions such as calcium and magnesium from hard water can improve water quality, which is important but technically challenging. Nanofiltration (NF) has attracted much attention because of its efficiency, environmental friendliness and low cost. However, common NF membranes with a singly (either positively or negatively) charged layer have insufficient water softening capacity. In this work, two types of dual-layer Janus charged polyamide NF membranes composed of oppositely charged inner and outer layers were developed for the first time by sequential electrospray polymerization strategy for efficient water softening. The effect of the microstructure of the dually charged barrier layer on the separation performance of divalent salt ions was explored. Detailed mechanistic studies revealed that the microstructure of the outer layer of the barrier layer played a crucial role in the ion separation of the Janus membrane due to its control of the reverse transport of ions. Janus charged polyamide NF membrane with a loose outer layer exhibited better water softening performance (93.6% of hardness removed) compared to the singly charged NF membranes due to the simultaneous dual electrostatic effect and no ion reverse transport confinement. This Janus charged NF membrane also possessed good antifouling performance, mainly due to its negatively charged outer layers. The mechanistic insights gained in this study reveal the huge potential of microstructural design toward high-performance Janus charged NF membranes, and provide important guidance on the future development of high-efficiency water softening NF membranes.
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Nano Today
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2022
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IF 18.962
Jing Ruan,Fang Li,Hao Tian,Jie Yu,Hongpei Deng,Shengfang Ge,Kam W. Leong
Photodynamic therapy (PDT) relies on the generation of cytotoxic singlet oxygen ( 1 O 2 ) from an otherwise nontoxic photosensitizer accumulated in tumor tissue to treat cancer. However, PDT of uveal melanoma, the most common adult intraocular cancer, using potent porphyrin derivatives as photosensitizers has shown limited therapeutic efficacy due to low bioavailability and 1 O 2 yield. Here, considering the uveal melanoma microenvironment of endogenous melanin , which has a high absorption between 300 nm and 500 nm, we design a carbon dot-based photosensitizer that uses multiple different pathways to generate 1 O 2 . We conjugated gadolinium (Gd)-doped carbon dots (Gd@Cdots) with the porphyrin-derivatives chlorin e6 (Ce6) and verteporfin (Ver). The resulting nanoparticle (Gd@Cdots/Ce6/Ver) generates 1 O 2 under UV irradiation mainly in cascade FRET from Gd@Cdots to Ce6 and then to Ver; the photoactivated Ver, in turn, transfers its energy to 3 O 2 to generate 1 O 2 . In human uveal melanoma MUM-2B cells in vitro, Gd@Cdots/Ce6/Ver generated 7.1- and 2.4-fold higher production of hydroxyl radical (·OH) and superoxide anions (O 2 - ·) following UV irradiation than Gd@Cdots, indicating greater induction of tumor cell apoptosis. In an orthotopic mouse model of uveal melanoma, PDT with Gd@Cdots/Ce6/Ver showed the same trend of superior potency in tumor growth inhibition. This photosensitizer design improves the PDT efficacy of uveal melanoma by enhancing the production of 1 O 2 and should be of broad utility to other PDT.
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ANALYTICAL CHEMISTRY
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2022
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IF 8.008
Yu Wang,Yu Xue,Qilin Zhao,Shuang Wang,Jian Sun,Xiurong Yang
Alzheimer’s disease (AD) is a common chronic neurodegenerative disease that manifests as cognitive impairment and behavioral deficits and severely threatens the health of the elderly. Acetylcholinesterase (AChE) plays a vital role in biological signaling and is an essential target for the early diagnosis and treatment of AD. Herein, 2D Zn-TCPP(Fe) nanosheets (NSs) employing Zn2+ and Fe-bound tetrakis(4-carboxyphenyl)porphyrin ligands were prepared through a surfactant-assisted synthetic method. The ultrathin two-dimensional (2D) metal–organic framework structures exhibited high peroxidase-like activity, which allowed the catalysis of the H2O2-initiated oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidized TMB (ox-TMB). Such catalytic performance inspired us to develop a convenient, rapid, and sensitive acetylcholinesterase activity assay, during which AChE can catalyze the substrate acetylthiocholine (ATCh) to produce thiocholine (TCh), and TCh could especially enable the degradation of 2D Zn-TCPP(Fe) NSs accompanied by the reduction of ox-TMB production. Our proposed sensing system exhibited favorable selectivity and sensitivity (LOD of 0.029 mU/mL) and has excellent potential to evaluate AChE activity in human serum samples and to screen AChE inhibitors. This colorimetric assay could provide an alternative pathway for early diagnosis and drug screening of AD, facilitating the development of AD therapy.
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CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2022
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IF 1.193
Yunxiao WANG,Dongyu GU,Chang LIU,Shanshan TANG,Shuai WANG,Yi WANG,Yi YANG
The tender leaves of Toona sinensis not only taste delicious, but also have a wide range of biological activities, including strong antioxidant activity. In this study, a method based on liquid-liquid-refining extraction and bioassay-guided separation were established and developed for enrichment, analysis and identification of antioxidant components in T. sinensis . Two main antioxidants, 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG) and ethyl gallate (EG) with strong antioxidant capacity were responsible for the antioxidant activity of T. sinensis . The EC 50 of EG scavenging ABTS· + and DPPH· were 22.53 ± 0.25 µM and 38.43 ± 0.66 µM, and the EC 50 of PGG were 13.72 ± 0.17 µM and 17.33 ± 0.21 µM. The thermodynamic parameters of various pathways indicated that single electron transfer followed by proton transfer (SET-PT) was the main antioxidant mechanism of PGG and EG. Furthermore, the results of frontier molecular orbital theory and reaction kinetics showed that PGG had low energy gap and reaction energy barrier, which was the fundamental reason why its antioxidant activity was better than EG.
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JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
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2022
·
IF 6.437
Fupeng Huang,Dongmei Bi,Shanjian Liu,Weiming Yi,Jiawen Zhang,Di Yao
Biochar contains environmentally persistent free radicals (EPFRs), a class of pollutants whose regulation and influencing factors need to be studied. In this study, electron paramagnetic resonance (EPR) signals were observed for biochar samples obtained by the pyrolysis of wheat straw, corn straw, peanut shell, and cotton stalk. The effects of pyrolysis temperature, residence time, and alkali/alkaline earth metals (AAEMs) on EPFRs in biochar were investigated. The adsorption effect of diethyl phthalate (DEP) on biochar obtained from the pyrolysis of wheat straw impregnated with K, Na, Ca, and Mg was also studied. The concentration of EPFRs increased first and then decreased upon increasing the pyrolysis temperature within the rage of 300–700 ℃, conforming to a Gaussian fit. Residence time experiments showed that the concentration of EPFRs was the highest at a residence time of 10 min. The effect of AAEMs on the EPFRs concentration followed the order Mg > K > Ca > Na due to the catalysis of a secondary reaction. Because of hole-filling and electrostatic attraction, biochar obtained by pyrolysis of wheat straw impregnated with MgCl 2 (WS Mg ) had the highest DEP removal rate of 95.969%. DEP removal efficiency of the five adsorbents followed the order WS Mg > WS Ca (88.701%) > WS (68.867%) > WS Na (53.852%) > WS K (50.845%). Hydroxyl radicals (•OH) signals were detected in these five adsorption systems, showing that EPFRs could activated H 2 O 2 to produce •OH. However, the five •OH signals had similar intensities, indicating that AAEMs had not significantly effects on the process of activation of H 2 O 2 to produce •OH by EPFRs. The results of this study are of great significance for understanding the formation and regulation of EPFRs and the utilization of biochar.
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Antioxidants
·
2022
·Vol. 11
·期 11
·2145
·
IF 7.675
Yuanfang Chen,Yuwei Yang,Haikang Tang,Ziqi Zhang,Xiaoliang Zhou,Wenqing Xu
Radiation exposure can immediately trigger a burst of reactive oxygen species (ROS), which can induce severe cell death and long-term tissue damage. Therefore, instantaneous release of sufficient radioprotective drugs is vital to neutralize those accumulated ROS in IR-exposed areas. To achieve this goal, we designed, synthesized, and evaluated a novel oral ROS-responsive radioprotective compound (M1) with high biocompatibility and efficient ROS-scavenging ability to act as a promising oral drug for radiation protection. The compound is stably present in acidic environments and is hydrolyzed in the intestine to form active molecules rich in thiols. M1 can significantly remove cellular ROS and reduce DNA damage induced by γ-ray radiation. An in vivo experiment showed that oral administration of M1 effectively alleviates acute radiation-induced intestinal injury. Immunohistochemical staining showed that M1 improved cell proliferation, reduced cell apoptosis, and enhanced the epithelial integrity of intestinal crypts. This study provides a promising oral ROS-sensitive agent for acute intestinal radiation syndrome.Keywords:ROS response;aminothiols;oral administration;radioprotection;intestinal damage
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