ACS Omega
·
2018
·
IF 0
Changfen Bi,Yulu Liang,Lijin Shen,Shanshan Tian,Kai Zhang,Yiliang Li,Xiwen He,Langxing Chen,Yukui Zhang
Efficient enrichment glycoproteins/glycopeptides from complex biological solutions are very important in the biomedical sciences, in particular biomarker research. In this work, the high hydrophilic polyethylenimine conjugated polymaltose polymer brushes functionalized magnetic Fe3O4 nanoparticles (NPs) denoted as Fe3O4–PEI–pMaltose were designed and synthesized via a simple two-step modification. The obtained superhydrophilic Fe3O4–PEI–pMaltose NPs displayed outstanding advantages in the enrichment of N-linked glycopeptides, including high selectivity (1:100, mass ratios of HRP and bovine serum albumin (BSA) digest), low detection limit (10 fmol), large binding capacity (200 mg/g), and high enrichment recovery (above 85%). The above-mentioned excellent performance of novel Fe3O4–PEI–pMaltose NPs was attributed to graft of maltose polymer brushes and efficient assembly strategy. Moreover, Fe3O4–PEI–pMaltose NPs were further utilized to selectively enrich glycopeptides from human renal mesangial cell (HRMC, 200 μg) tryptic digest, and 449 N-linked glycopeptides, representing 323 different glycoproteins and 476 glycosylation sites, were identified. It was expected that the as-synthesized Fe3O4–PEI–pMaltose NPs, possessing excellent performance (high binding capacity, good selectivity, low detection limit, high enrichment recovery, and easy magnetic separation) coupled to a facile preparation procedure, have a huge potential in N-glycosylation proteome analysis of complex biological samples.
✓ 已验证
DOI
PubMed
ANALYST
·
2020
·Vol. 146
·期 3
·896-903
·
IF 3.978
Xiaolei Xie,Yufei Wang,Xiaobin Zhou,Junyang Chen,Mengke Wang,Xingguang Su
Single-atom nanozymes have drawn wide attention in bio-sensing for their remarkable merits such as low cost, high stability, and maximum atom utilization. Herein, a colorimetric strategy based on Fe–N–C single-atom nanozymes (Fe/NC-SAs) was established for the detection of alkaline phosphatase (ALP) activity. The Fe/NC-SAs prepared by pyrolysis have excellent peroxidase-like activity and can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue color product in the presence of hydrogen peroxide (H2O2). When ascorbic acid (AA) is added to the system, the blue color fades, and the absorbance has a linear relationship with the concentration of AA. Alkaline phosphatase (ALP) can catalyze the hydrolysis of ascorbic acid 2-phosphate (AAP) to produce AA. Thus, a strategy based on Fe/NC-SAs for the detection of ALP activity was established, which provided a linear range of 0.1–1.5 U L−1 and a limit of detection as low as 0.05 U L−1. Besides, Fe/NC-SAs showed high stability under harsh conditions. Moreover, an Fe/NC-SA-based assay was successfully validated using human serum samples for ALP determination with satisfactory results, and has broad prospects in the field of biosensing.
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Jiujun Wang,Ya Wu,Qinghua Wu,Li Li,Yun Wang,Hualin Yang
Melamine has a high nitrogen content thus is often illegally added to milk powder to phonily boost the protein percentage composition. Milk products containing melamine are harmful to babies and children. In the present work, a simple, label-free and turn-on fluorescence method to detect melamine with a high sensitivity and selectivity has been developed. The N -methylmesoporphyrin IX (NMM)/G-quadruplex structure is used as a signal reporter in this method to realize label-free detection of melamine. The detection limit of this method is 12.5 nM, which is much less than the maximum level permitted for infant formulas. The linear range is 0.05–1 μM ( R 2 = 0.98). The selectivity of this method is also very satisfactory. Furthermore, this method can be successfully applied for detection melamine in raw milk and milk powder.
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yuqi Zhang,Yaxuan Zhen,Chunying Zhu,Taotao Fu,Youguang Ma
Chemical absorption has become the most mature and widely used CO 2 capture technology to address CO 2 emissions. Phase change absorbents based on mixed amines combine the advantages of individual amines and reduce regeneration energy consumption. A novel phase change absorbent system based on mixed amine was developed in this study and its CO 2 capture and desorption performance was investigated. The system comprises monoethanolamine (MEA), N-methyldiethanolamine (MDEA), n-butanol and water. Gas-liquid absorption experiments in the microchannel revealed that the flow patterns of the rich phase changed successively through homogeneous flow, irregular droplet flow, tubular-droplet flow and liquid slug flow as the gas-liquid ratio was increased. This transition was influenced by internal circulation, phase change and viscosity, causing the absorption rate to rise initially, then increase slowly before eventually declining. The desorption performance of the saturated rich phase in the MEA-MDEA-n-butanol-H 2 O system was significantly superior to that of the 30 wt% MEA aqueous solution. Comprehensively, the optimal mass ratio of MEA-MDEA-n-butanol-H 2 O was identified as 1.2:1.8:4:3, achieving a higher desorption rate and lower energy consumption. Desorption efficiency and cyclic capacity were 1.8 times those of the 30 wt% MEA aqueous solution, indicating promising application potential.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2025
·
IF 4.3
Jin Li,Yang Wang,Tianxing Wu,Xiujing Xing,Limin Li,Wei Xiong,Hao Li
Electrocatalytic reduction of nitrate for the synthesis of high-value chemical hydroxylamine (NH 2 OH) represents a sustainable pathway that combines the dual functions of green synthesis and wastewater treatment. However, the selective control of the process is extremely challenging, mainly due to the susceptibility of nitrate to over-reduction during the reaction, leading to uncontrolled conversion of the target product NH 2 OH to the thermodynamically stable product ammonia (NH 3 ). In this paper, the Cu-PDA MOF precursor was synthesized using the emulsion method, and ZIF-8 was introduced to form the structure of Cu-PDA@ZIF-8. Subsequently, the nitrogen-carbon-doped Cu-ZnO/N-C catalysts were formed by calcination under N 2 atmosphere. The synthesized catalyst effective improved NH 2 OH selectivity during nitrate reduction. The NH 2 OH yield and FE efficiency of Cu-ZnO/N-C reached 564.97 μmol h −1 mg cat −1 and 46.64 %, respectively, under neutral electrolyte conditions. Cu, as the main component of the catalyst, plays a dominant role in hydroxylamine by electrocatalytic nitrate reduction to promote the reaction in the stage of NO 3 – →*NH 2 OH. Zn, on the other hand, plays a key role in the phase *NH 2 OH → NH 2 OH, selectively generating the product NH 2 OH and blocking the excessive reduction of NH 2 OH to NH 3 . Additionally, this study achieved tandem catalytic selective generation of NH 2 OH at copper and zinc heterogeneous interfaces and offers a potential approach and reference for applications involving the nitrate reduction reaction.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Energy Storage
·
2025
·
IF 9.8
Jianfeng Hu,Siyang Wang,Jianbing He,Peng Xie,Zhengguo Zhang
To address the global energy crisis and achieve carbon neutrality goals, the development of efficient building thermal management technologies is imperative. Phase change materials (PCMs) have gained significant attention due to their high latent heat capacity. However, their practical application is constrained by limitations in packaging stability and mechanical properties. In this study, we developed a novel composite aerogel by constructing a three-dimensional porous matrix from biomass-derived guar gum (GG) and polyvinyl alcohol (PVA), which was then integrated with polyurea-encapsulated n-eicosane phase change microcapsules (MC) via freeze-drying. The synergistic combination of this tailored GG/PVA scaffold and the MC filler was systematically investigated. The results reveal that an optimized GG:PVA ratio of 1:1 confers exceptional morphological stability (volume shrinkage of 18.75 %) and mechanical strength (compression modulus of 857 kPa for GP@MC-5). The composite's enthalpy efficiency directly correlates with MC content, achieving a high relative enthalpy efficiency exceeding 96.26 %, a latent heat of 160.56 J/g, and a low thermal conductivity of 0.06452 W/(m·K). The material exhibits remarkable reliability, maintaining stable phase change enthalpy and microstructure after 100 thermal cycles, with a thermal decomposition temperature above 150 °C and a leakage rate below 1 %. Owing to its optimized structure and multifunctionality, the composite aerogel demonstrates outstanding thermal management performance. This work highlights its potential for application in energy-efficient buildings. Furthermore, its lightweight and flexible nature suggests promising utility in smart textiles for personal thermal management.
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Pharmacology
·
2020
·
IF 4.225
Gao Chengcheng,Sun Xin,Wu Zhipan,Yuan Huahua,Han Haote,Huang Hongliang,Shu Yuhan,Xu Mengting,Gao Ruilan,Li Shouxin,Zhang Jianbin,Tian Jingkui
Introduction: The leaves of Morus alba L is a traditional Chinese medicine widely applied in lung diseases. Moracin N (MAN), a secondary metabolite extracted form the leaves of Morus alba L, is a potent anticancer agent. But its molecular mechanism remains unveiled.Objective: In this study, we aimed to examine the effect of MAN on human lung cancer and reveal the underlying molecular mechanism.Methods: MTT assay was conducted to measure cell viability. Annexin V-FITC/PI staining was used to detect cell apoptosis. Confocal microscope was performed to determine the formation of autophagosomes and autolysosomes. Flow cytometry was performed to quantify cell death. Western blotting was used to determine the related-signaling pathway.Results: In the present study, we demonstrated for the first time that MAN inhibitd cell proliferation and induced cell apoptosis in human non-small-cell lung carcinoma (NSCLC) cells. We found that MAN treatment dysregulated mitochondrial function and led to mitochondrial apoptosis in A549 and PC9 cells. Meanwhile, MAN enhanced autophagy flux by the increase of autophagosome formation, the fusion of autophagsomes and lysosomes and lysosomal function. Moreover, mTOR signaling pathway, a classical pathway regualting autophagy, was inhibited by MAN in a time- and dose-dependent mannner, resulting in autophagy induction. Interestingly, autophagy inhibition by CQ or Atg5 knockdown attenuated cell apoptosis by MAN, indicating that autophagy serves as cell death. Furthermore, autophagy-mediated cell death by MAN can be blocked by reactive oxygen species (ROS) scavenger NAC, indicating that ROS accumulation is the inducing factor of apoptosis and autophagy. In summary, we revealed the molecular mechanism of MAN against lung cancer through apoptosis and autophagy, suggesting that MAN might be a novel therapeutic agent for NSCLC treatment.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Haiyan Wang,Ximei Zhang,Peng Kang,Xiuxiu Cui,Guo Hao,Zhongfu Wang,Bei Han,Xin Lv,Jing Zhang,Wupeng Ge
Oligosaccharides and N/O-glycans are abundant in human milk and have numerous biological functions (for instance sialylated glycans provide sialic acid for the growth of infant brains), but their variation trends during lactation need further exploration. Qualitative and quantitative analyses of oligosaccharides and N/O-glycans in human milk at different lactation stages (from 7 days to 8 months) were performed using UHPLC-ESI-MS/MS. Thirty-four oligosaccharides, twenty-three N-glycans, and six O-glycans were identified. Oligosaccharides showed the highest abundance in human colostrum and decreased with the progression of lactation, and the abundance of N/O-glycans fluctuated as lactation progressed, while a high abundance of sialylated oligosaccharides and sialylated N/O-glycans was observed in human colostrum. These findings provide evidence for breastfeeding support and contribute to the development of infant formula supplemented with human milk glycans.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
·
IF 5.8
Tie Liu
The geometric design of atomically dispersed 'framework-active sites' grafted with carbonaceous materials to establish a well-connected three-dimensional network represents a compelling strategy for enhancing the performance of lithium-oxygen batteries, particularly in terms of cyclability. This paper introduces a simple approach that utilizes self-assembly heterogeneous nucleation to fabricate discrete bimetallic (Co, Ni) micron lattices grafted onto CNTs, exhibiting commendable surface properties. This intriguing hierarchical structure optimally harnesses the profound structural effects of CNTs weaving matrix, thereby imparting essential properties to the cathode, including high catalytic activity, intrinsically accessible active sites, and continuous porosity to accommodate discharge products. And such binary metal-nitride grafted configuration is significant in mitigating the embarrassing presence of nitrides (low conductivity) and carbon material (inferior activity). Consequently, cell measurement results indicate that a comparable capacity of 8599.7 mA h g⁻¹ and cyclic performance exceeding 200 cycles are achieved in a semi-solid state battery, outperforming commercially available RuC catalysts. And the DFT calculations further demonstrate the strong electronic coupling transfer and exceptional bifunctional activity observed in this binary metal-nitride sample. Therefore, this work exemplifies both steerable preparation techniques and the considerable potential inherent in geometric design for bimetallic sites grafted with carbonaceous material-based catalysts as they transition from Li-O 2 to Li-air batteries.
✓ 已验证
DOI
高影响因子
JOURNAL OF POWER SOURCES
·
2024
·
IF 8.1
Siyu Jin,Yuhua Xie,Hongyuan Xu,Yanping Luo,Jing Li,Weiwei Cai,Hui Xu,Hong Jin
The sluggish redox reactions and the shuttle effect associated with lithium polysulfides significantly impede the advancement of LSBs. To solve these problems, we synthesized CoS-FeS heterostructures supported by N-doped carbon nanocage (CoS-FeS@NC). The kinetic analysis shows that the enriched heterojunctions strengthens the bonding of host with polysulfides, thus promotes the redox reaction kinetics of polysulfides. XPS analysis reveals valence state of cobalt is elevated following incorporation of iron. This increase is attributed to the transfer of electrons from Co 2+ /Co 3+ to Fe 3+ . The configuration of the cationic valence electrons drives this electron transfer, which in turn improves the catalytic efficiency of the CoS-FeS@NC composites. Leveraging these advantages, the S@CoS-FeS/NC cathode demonstrates an exceptional ability to adsorb lithium polysulfides (LiPSs), along with rapid reaction kinetics and enduring stability in cycling (reversible capacity of 638 mAh g −1 at 0.2 C after 200 cycles). Remarkably, even when subjected to a charge/discharge rate as high as 1 C, the cathode sustains a capacity of 619 mAh g −1 and it keeps 542 mAh g −1 at 1 C after 1000 cycles at a degradation rate of merely 0.05 % for each cycle. This offers a feasible strategy to thoughtfully fabricate bimetallic sulfide heterostructures and fosters advancements in various electrochemical energy storage and conversion applications.
✓ 已验证
DOI
高影响因子
JACS Au
·
2024
·
IF 8.5
Xixi Liu,Liang Huang,Yurong He,Peng Zhou,Xuedan Song,Zehui Zhang
It remains challenging to construct C═N bonds due to their facile hydrogenation. Herein, a single Co atom catalyst was discovered to be active for the selective construction of C═N bonds toward the synthesis of imines and N-heterocycles via reductive coupling of nitroarenes with various alcohols, including inert aliphatic ones. DFT calculations and experimental data revealed that the transfer hydrogenation proceeded via the intramolecular hydride transfer and the transfer of H from the α-Csp3-H bond to the nitro group was the rate-determining step. The single Co atoms served as a bridge to transfer the electrons from the catalyst to the adsorbed alcohol molecules, resulting in the activation of the α-Csp3-H bond. Unlike metal nanoparticles, the C═N bonds in imine products can be reserved due to the large steric hindrance from substituents on C and N. DFT calculation also confirmed that transfer hydrogenation of the C═N bonds in imines is thermodynamically unfavored with a much higher energy barrier compared with the transfer hydrogenation of the –NO2 group (1.47 vs 1.15 eV).
✓ 已验证
DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2021
·Vol. 33
·期 6
·065409
·
IF 3.874
Wenwen Zhang,Ximeng Zhao,Weixing Niu,Hang Yu,Tongtao Wan,Guihua Liu,Dongsheng Zhang,Yanji Wang
The slow kinetic of oxygen reduction reaction (ORR) hampers the practical application of energy conversion devices, such as fuel cells, metal-air batteries. Here, an efficient ORR electrocatalyst consists of Co, Ni co-decorated nitrogen-doped double shell hollow carbon cage (Ni–Co@NHC) was fabricated by pyrolyzing Ni-doped polydopamine wrapped ZIF-67. During the preparation, polydopamine served as a protective layer can effectively prevent the aggregation of Co and Ni nanoparticles during the pyrolysis process, and at the same time forming a carbon layer to grow a double layer carbon cage. This unique hollow structure endows the catalyst with a high specific surface area as well as more exposed active sites. Also benefited from the synergistic effect between Ni and Co nanoparticles, the Ni–Co@NHC catalyst leads to an outstanding ORR performance of half-wave potential (E1/2, 0.862 V), outperforms that of commercial Pt/C catalyst. Additionally, when Ni–Co@NHC was used in the cathode for the zinc-air battery, the cell exhibits high power density (108 mW cm−2) and high specific capacity (806 mAh g−1) at 20 mA cm−2 outperforming Pt/C. This work offers a promising design strategy for the development of high-performance ORR electrocatalysts.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）