NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
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2025
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IF 3.1
Liu Wencheng,Liu Rifu,Yang Yue,Cheng Lixue,Feng Xiaobing,Li Qinglei
This study investigated the potential therapeutic effect of kaempferol on osteoporosis by regulating bone homeostasis through the YAP. The ovariectomy model was constructed. The changes in bone and liver tissue were observed through HE staining. Bone metabolism and inflammatory markers were quantitatively analyzed using RT-qPCR and ELISA. Changes in the YAP/NF-κB signaling pathway were detected through Western blot and immunofluorescence. In vitro, the activity and secretion levels of MC3 T3-E1-differentiated osteoblasts were assessed using CCK- 8 and ELISA. The effects of osteoblast-secreted factors on osteoclast-induced differentiation were analyzed using alizarin red staining, phalloidin staining, ELISA, TRAP staining, and SEM. Finally, the regulatory effects of kaempferol on the expression of bone homeostasis in osteoclasts were examined by immunofluorescence and Western blot. Kaempferol treatment improved trabecular bone structure, increased bone density, and modulated bone metabolism by enhancing OPG expression and suppressing TRAP, NFATC1, and RANKL levels. Kaempferol inhibited the activation of the NF-κB pathway and increased YAP expression, reducing inflammatory factors. In vitro, kaempferol promoted osteoblast differentiation and inhibited osteoclast activity. These effects were most pronounced at higher kaempferol doses and were associated with improved bone remodeling and reduced bone resorption. Kaempferol exhibits significant potential for osteoporosis treatment by regulating bone homeostasis, mitigating inflammation through modulating the YAP.
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高影响因子
IONICS
·
2025
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IF 2.6
Chen Peng,Wang Yan,Cai Wangfeng
In this study, we successfully synthesized a novel flower-like composite V 2 O 5 @CoV-LDH, which was directly anchored onto nickel foam through a two-step hydrothermal method to enhance surface area and stability. The synthesized flower-like composite V 2 O 5 @CoV-LDH features V 2 O 5 as the first layer and CoV-LDH as the second layer, effectively merging the high capacitance and energy density of V 2 O 5 with the large surface area and multivalent states of CoV-LDH. Moreover, this unique bilayer composite structure, directly anchored onto nickel foam, significantly enhances the overall stability of the electrode material. This design demonstrates outstanding performancedue to a strong synergistic effect, which provides the composite with more reactive sites, shorter ion transport pathways, and enhanced cycling stability. The test results show that the V 2 O 5 @CoV-LDH electrode achieves a high specific capacitance of 1822 F g −1 at 1 A g −1 and retains 85.99% of its capacitance after 5000 charge–discharge cycles, demonstrating excellent stability. The asymmetric supercapacitor assembled with the V 2 O 5 @CoV-LDH electrode and activated carbon electrode delivers an energy density of 59.85 Wh kg −1 at a power density of 900 W kg −1 . After 10,000 cycles at 5 A g −1 , the specific capacitance remains at 88%, indicating promising potential for practical applications.
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Reaction Kinetics Mechanisms and Catalysis
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2024
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IF 1.7
Gao Xinglong,Qiu Feifei,Xie Hongzhao,Chen Xiaopeng,Wu Chenghong,Chen Yutong,Liang Jiezhen,Wang Linlin
In the present work, efficient catalytic hydrogenation of acrylic rosin (AR) were extensively investigated. Qualitative and quantitative analysis of AR and hydrogenated acrylic rosin (HAR) were carried out by GC and GC–MS, and the reaction pathway network about catalytic hydrogenation of AR was constructed. In addition, a Pd/C catalyst with well-dispersed ultrafine nano-palladium was prepared by impregnation method using Pd(NO 3 ) 2 ·2H 2 O precursor and put into use for the catalytic hydrogenation of AR. The structural properties of the catalyst were measured via XRD, TEM, ICP-OES, XPS, and BET. The characterizations showed that the ultrafine Pd nanoparticles (NPs) with an average Pd NPs size of 2.29 nm and consisting of 3.1% Pd were equably loaded on the surface of activated carbon. Benefiting from well-dispersed ultrafine Pd NPs, the catalyst exhibits outstanding activity in the hydrogenation of AR at 180 °C and 5 MPa, which shows 1.9 times superior activity than a commercial 10% Pd/C. The quality of prepared HAR is similar to the commercial HAR (Gardner color < 1, acid value of 235 mg KOH/g, softening point of 129 °C, 35% conversion rate of acrylpimaric acids). Graphical abstract
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JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-19
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IF 12.6
Zhu Junyi,Zhang Yijia,Sun Yinuo,Yu Fangzheng,Lu Yang,Hu Qianqian,Guo Jiali,Zhang Haijuan,Chen Tianling,Lian Feifei,Wang Jian,Li Xiaokun,Xiao Jian
The applications of nanomaterials in regenerative medicine encompass a broad spectrum. The functional nanomaterials, such as Prussian blue and its derivative nanoparticles, exhibit potent anti-inflammatory and antioxidant properties. By combining it with the corresponding scaffold carrier, the fusion of nanomaterials and biotherapy can be achieved, thereby providing a potential avenue for clinical treatment. The present study demonstrates the fabrication of a Mesoporous Prussian blue nanoparticles (MPBN) functionalized Inverse Opal Film (IOF) neuroconduit for peripheral nerve repair through reverse replication and freeze-drying techniques. The binding of MPBN to the neuroconduit can effectively decreasing reactive oxygen species and inflammatory factors in the vicinity of the residual nerve, thereby providing protective effects on the damaged nerve. Furthermore, comprehensive behavioral, electrophysiological, and pathological analyses unequivocally substantiate the efficacy of MPBN in increasing nerve structure regeneration and ameliorating denervation-induced myopathy. Moreover, MPBN enhances the antioxidant capacity of Schwann cells by activating the AMPK/SIRT1/PGC-1 pathway. The findings suggest that MPBN, a biocompatible nanoparticle, can safeguard damaged nerves by optimizing the microenvironment surrounding nerve cells and augmenting the antioxidant capacity of nerve cells, thereby facilitating nerve regeneration and repair. This also establishes a theoretical foundation for exploring the integration and clinical translation between nanomaterials and biotherapy.
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高影响因子
Journal of Translational Medicine
·
2025
·Vol. 23
·期 1
·1-19
·
IF 7.5
Han Ning,Yu Na,Yu Li
Background Diabetic retinopathy (DR) is the common cause of diabetic vascular complications and it causes blindness. Until now, there are still some patients with DR who lack effective treatment. Tripartite motif containing 44 (TRIM44) has been shown to play a significant role in endothelial cells. However, the role of TRIM44 in DR remains unknown. Methods Diabetes was induced in rats through the administration of an intraperitoneal injection of 65 mg/kg of streptozotocin (STZ). Rat retinal microvascular endothelial cells (RMECs) were subjected to stimulation under high glucose (HG) conditions. A thorough proteomic investigation and bioinformatic analysis were performed to identify the differentially expressed proteins (DEPs) in rat RMECs after blocking TRIM44. A dual luciferase reporter assay was employed to assess the luciferase activity of TRIM44. Results TRIM44 was highly expressed in the retinal tissues of rats with diabetes and HG-induced RMECs. In vivo assays suggested that TRIM44 silencing improved the pathological alterations of DR rats as demonstrated by the downregulated expression of isolectin-B4 and VEGFA, along with a decrease in acellular capillaries within the retinal tissues. Knockdown of TRIM44 markedly reduced cell viability, proliferation, migration, invasion, and angiogenesis in HG-evoked RMECs. Mechanistically, TRIM44 was demonstrated to be activated transcriptionally by KLF transcription factor 9 (KLF9), a known facilitator of angiogenesis in DR. In HG-induced cells, the loss of TRIM44 resulted in the reverse of the endothelial cell function caused by KLF9 overexpression. After the comprehensive analysis, 64 upregulated and 38 downregulated DEPs were screened out for a series of functional enrichment analyses. Conclusions Collectively, this study demonstrates that TRIM44 knockdown suppressed diabetes-induced retinal vascular dysfunction in DR.
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ACTA PHARMACOLOGICA SINICA
·
2025
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IF 8.4
Wang Hao,Zhang Li,Yang Wan-yue,Ji Xiao-yi,Gao An-qi,Wei Yi-hong,Ding Xin,Kang Yue,Ding Jian-hua,Fan Yi,Lu Ming,Hu Gang
Obesity increases the risk of depression. Evidence shows that peripheral inflammation, glycemic dysregulation, and hyperactivity within the hypothalamic-pituitary-adrenal axis are implicated in both obesity and depression. In this study we investigated the impact of visceral adipose tissue (VAT), a crucial characteristic of obesity, on stress susceptibility in obese mice. Age-matched mice were fed with chow diet (CD) or high-fat diet (HFD), respectively, for 12 weeks. CD mice were deprived of VAT and received transplantation of VAT from HFD mice (TransHFD) or CD mice (TransCD). Extracellular vesicles (EVs) were prepared from VAT of CD or HFD mice, and intravenously injected (100 μg, 4 times in 2 weeks) in naïve mice or injected into hippocampus (5 μg, 4 times in 2 weeks) through implanted bilateral cannula. Depression-like behaviors were assessed 14 days after transplantation. We showed that HFD mice exhibited significantly higher body weight gain and impaired insulin and glucose tolerance, accompanied by increased stress susceptibility. Transplantation of VAT or VAT-derived EVs from HFD mice caused synaptic damage and promoted stress susceptibility in recipient mice. Through inhibiting miRNA biogenesis in the VAT and miRNA sequencing analysis, we demonstrated that miR-140-5p was significantly upregulated in both VAT-EVs and hippocampus of HFD mice. Overexpression of hippocampal miR-140-5p in naïve mice not only facilitated acute stress-induced depression-like behaviors, but also decreased hippocampal CREB-BDNF signaling cascade and synaptic plasticity. Conversely, knockdown of miR-140-5p in the VAT, VAT-EVs or hippocampus of HFD mice protected against acute stress, reducing stress susceptibility that were mediated via CREB-BDNF pathway. In summary, VAT-EVs or the cargo miRNAs in obese mice promote synaptic damage and stress susceptibility, providing potential therapeutic targets for metabolism-related affective disorders.
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高影响因子
Reproductive Sciences
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2025
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IF 2.5
Ma Qianwen,Wu Lifei,Wu Jianfei,Ni Binfei,Wang Jiajia,Song Shiyan
Objective To investigate the mechanism in the progression of decreased ovarian reserve (DOR). Methods Three-month-old female SD rats were employed and randomly divided into the model group and the normal group. The model group was intraperitoneally injected with 4-vinylcyclohexene diepoxide (VCD). Thereafter, blood sample from the abdominal aorta was taken, and rats were sacrificed, and ovarian tissues were obtained by laparotomy. Results HE staining results revealed that the model group exhibited significantly reduced ovarian volume, increased follicular atresia, and decreased quantities of growing follicles and corpus luteum, thereby indicating degraded reserve function of ovarian. TEM images revealed that prominent autophagic vacuoles could be observed in the model group, accompanied by the mitochondria shrinkage and generation of the autophagosome. The expression of Pink1, Parkin, BNIP3L and LC3II genes in ovaries of the model group was significantly higher than those of the normal group ( P < 0.05). In addition, the protein expression of Pink1, Parkin, BNIP3L and LC3II in ovaries of the model group were higher than those of the normal group ( P < 0.05). The expression of Fe 2+ and GSH in oocytes of the model group was higher than those of the normal group ( P < 0.05). The expression of FTH1 and GPX4 in oocytes of the model group was significantly higher than those of the normal group ( P < 0.05). Conclusions Mitochondrial autophagy and ferroptosis may participate in the progression of decreased ovarian reserve (DOR).
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高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-13
·
IF 15.7
Bai Lishun,Xu Yan,Liu Yue,Zhang Danni,Zhang Shibin,Yang Wujie,Chang Zhi,Zhou Haoshen
The rapid evolution of portable electronics and electric vehicles necessitates batteries with high energy density, robust cycling stability, and fast charging capabilities. High-voltage cathodes, like LiNi 0.8 Co 0.1 Mn 0.1 O 2 (NCM-811), promise enhanced energy density but are hampered by poor stability and sluggish lithium-ion diffusion in conventional electrolytes. We introduce a metal-organic framework (MOF) liquid-infusion technique to fully integrate MOF liquid into the grain boundaries of NCM-811, creating a thoroughly coated cathode with a thin, rigid MOF Glass layer. The surface electrically non-conductive MOF Glass layer with 2.9 Å pore windows facilitating Li-ion pre-desolvation and enabling highly aggregative electrolyte formation inside the Glass channels, suppressing solvated Li-ion co-insertion and solvent decomposition. While the inner Glass layer composes of Li-ion conducting components and enhancing fast Li-ion diffusion. This functional structure effectively shields the cathode from particle cracking, CEI rupture, oxygen loss, and transition metal migration. As a result, Li | |Glass@NCM-811 cells demonstrate good rate capability and cycling stability even under high-charge rates and elevated voltages. Furthermore, we also achieve a 385 Wh kg -1 pouch-cell (19.579 g, for pouch-cell), showcasing the practical potential of this method. This straightforward and versatile strategy can be applied to other high-voltage cathodes like Li-rich manganese oxides and LiCoO 2 .
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高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 5
·1-13
·
IF 5.3
Zhang Jing,Yin Zhaojiang,Xiong Wei,Fan QiQi,Yu Fengyao,Liao Fusheng,Fan Hao,Qu Fei,Yu Qiangqiang
A novel, low-toxicity Ru-Ni-BPY metal–organic framework (MOF) nanomaterial was developed and a simple and sensitive dual-mode "on–off" ferric ions (Fe 3+ ) sensing platform based on this material constructed. The dual-mode detection method enabled cross-validation of the electrochemiluminescence (ECL) and fluorescent (FL) signals, enhancing accuracy and effectively minimizing errors. In linear ranges of 0.005–10 μM and 0.078–10 μM, Fe 3+ can dramatically quench the ECL and FL signals of the Ru-Ni-BPY MOF, with quenching rates of 91.42% and 91.31%, respectively, and detection limits of 0.1 nM and 9.3 nM, respectively. This ECL method not only achieved efficient detection of Fe 3+ in rat brain microdialysis fluid but also successfully enabled real-time FL detection of Fe 3+ in nude mice. Therefore, this platform has great potential for applications in environmental protection, life sciences, and early disease diagnosis. Graphical abstract
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Journal of Coatings Technology and Research
·
2024
·
IF 2.3
Chen Lvxin,Yu Zhijie,Fang Yinchun
Structural colorization as a new green coloring method has received more and more attention. However, structural colors with only color can no longer meet the actual needs. It is of great practical significance to develop functional structural colors with brilliant colors and special function. In this study, Cu 2 O single crystal spheres with different sizes were successfully prepared and were sprayed on the surface of polyester/cotton fabric to obtain bright purple, purple red, light blue, dark green, grass green, and orange–yellow structural colors. Due to the introduction of polyacrylate (PA) adhesive, Cu 2 O structural colorized fabrics had excellent abrasion and washing colorfastness and stability. Meanwhile, the Cu 2 O structural color has little influence on the original softness and air permeability of fabric. The structural colorized fabric showed excellent antibacterial properties against E. coli and S. aureus , and the antibacterial rates decreased with the increasing of particle sizes of Cu 2 O spheres. The large-scale preparation of uniform structural colors on fabric can be realized by the spraying method. The fine and complex structural color patterns can also be obtained by spraying Cu 2 O single crystal spheres, showing good industrial application prospects. Therefore, this study provides experimental basis for the development of functional structural colors with brilliant colors and excellent antibacterial properties. Graphical abstract
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高影响因子
PHARMACEUTICAL RESEARCH
·
2025
·
IF 4.3
Lu Tianshu,Wu Yiyang,Xiong Ping,Zhong Hao,Ding Yang,Li Haifeng,Ouyang Defang
Objective Amorphous solid dispersion (ASD) is widely utilized to enhance the solubility and bioavailability of water-insoluble drugs. However, conventional experimental approaches for ASD development are often resource-intensive and time-consuming. Machine learning (ML) algorithms have great potential to predict ASD formulations but face the challenge of extensive data to construct reliable models. Current study aims to predict the formation of both binary and ternary ASD by combined high-throughput screening (HTS) and ML approaches. Methods Micro-quantity HTS was conducted to generate 1272 binary and ternary solid dispersions using solvent evaporation method. The Powder X-Ray Diffraction (PXRD) was used to characterize the amorphous state of formulations. The results indicated that 188 formulations successfully formed amorphous solid dispersions (ASDs), while 1084 resulted in crystalline formations. Models development employed nested cross-validation with four algorithms: Light Gradient Boosting Machine (LGBM), Random Forest (RF), Support Vector Machine (SVM), and Multi-Layer Perceptron (MLP). Results The RF model for ASD formation achieved 96.7% accuracy on the in-house HTS dataset, with a precision of approximately 87.9% and an F1 score of 83.6%. Furthermore, the RF model trained with milligram-scale HTS experimental data could effectively predict the large-scale ASD formulations from the literature, highlighting its promise as a powerful tool for advancing ASD prediction. Conclusion In summary, the combination of HTS experiments and ML techniques provides a valuable reference framework for ASD development, greatly minimizing both time and material usage in the selection of formulations during the early stages of drug discovery with a limited quantity of API. Graphical The workflow of this study involves a micro-quantity HTS experiment that generated over a thousand homogeneous data points for ML model development. The RF model demonstrated strong performance on a large-scale external dataset for ASD prediction, significantly saving time and material usage in formulation development, particularly during early drug discovery.
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高影响因子
Frontiers of Chemical Science and Engineering
·
2024
·Vol. 18
·期 7
·1-11
·
IF 4.3
Chen Yi,Chen Shaowei,Shao Yan,Quan Cui,Gao Ningbo,Fan Xiaolei,Chen Huanhao
Electrified non-thermal plasma (NTP) catalytic hydrogenation is the promising alternative to the thermal counterparts, being able to be operated under mild conditions and compatible with green electricity/hydrogen. Rational design of the catalysts for such NTP-catalytic systems is one of the keys to improve the process efficiency. Here, we present the development of siliceous mesocellular foam (MCF) supported Cu catalysts for NTP-catalytic CO 2 hydrogenation to methanol. The findings show that the pristine MCF support with high specific surface area and large mesopore of 784 m 2 ·g −1 and ∼8.5 nm could promote the plasma discharging and the diffusion of species through its framework, outperforming other control porous materials (viz., silicalite-1, SiO 2 , and SBA-15). Compared to the NTP system employing the bare MCF, the inclusion of Cu and Zn in MCF (i.e., Cu 1 Zn 1 /MCF) promoted the methanol formation of the NTP-catalytic system with a higher space-time yield of methanol at ∼275 µmol·g cat −1 ·h −1 and a lower energy consumption of \(26.4\ \text{kJ}\cdot \text{mmol}_{\text{CH}_{3}\text{OH}}\!^{-1}\) (conversely, ∼225 µmol·g cat −1 ·h −1 and \(\sim71\ \text{kJ}\cdot \text{mmol}_{\text{CH}_{3}\text{OH}}\!^{-1}\) , respectively, for the bare MCF system at 10.1 kV). The findings suggest that inclusion of active metal sites (especially Zn species) could stabilize the CO 2 /CO-related intermediates to facilitate the surface reaction toward methanol formation.
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危险品化学品经营许可证（带存储）