Molecular Catalysis
·
2024
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IF 3.9
Xiangyu Zheng,Zhe Dou,Wenqiang Xiang,Wen Zhang,Ye Ni,Guochao Xu
N -substituted β-amino esters as an important class of chiral amines, are widely used as building blocks in pharmaceuticals. A novel imine reductase Sv IRED was identified through gene mining from Streptomyces viridochromogenes with high enantioselectivity of > 99 % ee (1 R , 2 S ), 95 % de ( cis:trans ), towards 2-oxocyclohexane-1-carboxylate and cyclopropylamine. Sv IRED exhibited the highest activity at pH 7.0 and 40 °C. A variety of amine donors could be used by Sv IRED and produce chiral N -substituted β-amino esters with different enantioselectivity. Employing methylamine ( i ) and pyrrolidine ( n ) as amine donors, Sv IRED exhibited reversed enantioselectivity of 23 % ee (1 S , 2 R ) and 79 % ee (1 S , 2 R ) respectively. Through alanine scanning, M183 and H244 were identified with remarkable roles in governing enantioselectivity towards i and n . Compared with WT Sv IRED, the enantioselectivity of M183A was inverted to ee value of >99 % (1 R , 2 S ) and de value of >99 % ( cis:trans ) towards i , and the enantioselectivity of H244A was inverted to ee value of 88 % (1 R , 2 S ) and de value of >99 % ( cis:trans ) towards n . Interaction analysis indicated the orientation of ester group was vital for manipulation of enantioselectivity. This study provides novel Imine reductase mutants for enantioselective synthesis of N -substituted β -amino esters with different amine donors.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Zhong-hao Zhang,Ying-hong Pei,Zhi-hao Duan,Tao Gao,Shi-ling Feng,Zi-zhong Tang,Yang-er Chen,Sheng-lin Hu,Shu Yuan,Wei Wang,Xiao-rong Yan,Ya-ying Pu,Ming Yuan
Alzheimer's disease (AD) is prevalent in the elderly, with amyloid-β (Aβ) playing a critical role in its progression. Polysaccharides have garnered increasing attention due to their low toxicity and diverse applications in alleviating AD-like symptoms. However, the potential of ginger leaf polysaccharide in mitigating AD-like symptoms has been rarely investigated. In this study, we isolated a polysaccharide (GLP1) from ginger leaf and evaluated its efficacy and underlying mechanisms in alleviating AD-like symptoms using Caenorhabditis elegans and PC12 cells. GLP1 ameliorated AD-like symptoms in C. elegans , as evidenced by a 41.50 % increase in head thrashing frequency and an 87.13 % increase in body bending frequency. Furthermore, GLP1 mitigated cognitive decline by 76.51 %. Additionally, GLP1 enhanced the activity of acetylcholinesterase in C. elegans and maintained the integrity of neural system function. Moreover, GLP1 improved the survival rate of PC12 cells under Aβ induction by activating the Wnt/β-catenin pathway, which also resulted in a reduction in the release of inflammatory factors, specifically IL-1β by 21.15 %, IL-6 by 39.98 %, and TNF-α by 19.66 %. Notably, FITC-labeled GLP1 could be absorbed by PC12 cells. These compelling findings underscored the therapeutic potential of GLP1 in alleviating Aβ-induced AD-like symptoms and supported the advancement of ginger leaf resource utilization.
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Nano Energy
·
2025
·
IF 17.1
Xiuzi Che,Huiqing Fan,Jiahao Cao,Yongbo Fan,Yuxin Jia,Yidi Duan,Qinghua Guo,Yafei Feng,Weijia Wang
Piezoelectric biomaterials can convert mechanical energy into electricity, which make them attractive for implant applications, such as sensing, energy harvesting, and medical devices. The β-glycine has excellent piezoelectric performance, but their practical use is hindered by instable crystal phase and water solubility. Here, we present a simple method for preparing poly L-lactide (PLLA) composite fibers encapsulating β-glycine nano crystals via coaxial electrospinning. The formation of β-glycine crystals is the synergistic outcome of the ionization of glycine solution under the electric field and the confinement of external PLLA solution, which enhances the piezoelectric performance of the film. The V oc , I sc , and power density of composite film reach 14 V, 0.94 μA, and 0.92 mW/cm 2 , respectively. In addition, PLLA shell stabilizes β-glycine, leading to a stable piezoelectric performance for device applications. The composite film can be ultrasonically driven to generate electric signals, and therefore functions well in promoting cell proliferation and bone tissue repairing in vivo . Moreover, the film can be naturally degraded after long-term implantation. This study provides a strategy to deal with the instability of β-glycine-based biodegradable piezoelectric films, and promotes the implant applications.
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FOOD AND CHEMICAL TOXICOLOGY
·
2025
·
IF 3.5
Yongjun Tang,Zhengning Feng,Chenlu Ma,Nian Jang,Xiaolong Chen,Yingxu He,Francis L. Martin,Hui Liu,Weiyi Pang
Breast cancer has a high global incidence, and benzo[ a ]pyrene (B[ a ]P) is considered a contributing factor that increases carcinogenic risk. This study examined B[ a ]P's oncogenic mechanisms in mammary epithelial cells. Chronic B[ a ]P exposure induced morphological changes and enhanced proliferative/clonogenic capacity in MCF-10A cells. Chronic B[ a ]P exposure altered gene expression in MCF-10A cells, revealing differential levels of circRNAs, lncRNAs, miRNAs, and mRNAs. qRT-PCR validation demonstrated strong alignment with RNA-seq results, ensuring sequencing reliability. Additionally, chronic B[ a ]P exposure upregulated the protein expression of AhR and ARNT, as well as TGF- β , pSmad2/3, and KRT14 , while increasing Vimentin expression and decreasing E-cadherin expression. Notably, treatment with the TGF- β inhibitor SB431542 reversed these protein expression changes in transformed cells. These results show that exposure to Chronic B[ a ]P induces MCF-10A cell transformation. The underlying mechanisms involve significant transcriptional alterations, AhR/ARNT expression regulation, TGF- β signaling pathway activation, KRT14 protein modulation, and EMT. Furthermore, Chronic B[ a ]P exposure may drive transformation through TGF- β modulation. Chronic B[ a ]P exposure promotes breast carcinogenesis, revealing mechanistic insights and potential preventive biomarkers.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Rui Fan,Xiaoqian Chen,Yanbo Qin,Caiyun Wang,Jinrui Du,Guofang Zhang,Xinyao Sun,Chun Li,Libo Liu,Jian He
This investigation focuses on examining an integrated approach employing hollow fiber membrane filtration combined with dialysis washing filtration. By systematically varying the low-temperature dissociation time following the addition of deionized water to dilute casein obtained through 50 °C microfiltration membrane separation, the β-casein (β-CN) to whey protein ratio in the permeate samples was modulated. Comparative analyses were conducted to evaluate β-CN purity, structural properties, and physicochemical-functional attributes across samples prepared with different dissociation periods. The optimal β-CN purity (70.96 %) was achieved following 36 h of dissociation treatment. The high-purity β-CN preparations exhibited superior functional performance, demonstrating enhanced foaming capacity and emulsification properties. Specifically, the emulsion activity index reached 20 m 2 /g and 70 % emulsion stability, whereas foaming capacity attained 113.70 % and 49.25 % foam stability. Throughout the low-temperature dissociation process, the particle size of the β-CN increased gradually, and the average particle size peaked at 86.67 nm at 48 h. This investigation seeks to optimize the β-CN isolation process while establishing technical parameters for industrial-scale production of β-CN across multiple purity grades. Meanwhile, a comprehensive evaluation was conducted to systematically characterize the physicochemical properties and functional performance of β-CN preparations with varying purity levels.
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npj Science of Food
·
2025
·Vol. 9
·期 1
·1-9
·
IF 7.8
Ye Xiang,Chen Wen,Yan Fujie,Zheng Xiaodong,Tu Pengcheng
In late-stage type 2 diabetes mellitus (T2DM), impaired islet β cell function leads to absolute insulin deficiency, thereby disrupting blood glucose homeostasis. GLP-1, an incretin hormone, stimulates insulin secretion from islet β cells post-meals. This study investigated the effects of anthocyanin cyanidin-3-O-glucoside (C3G) on GLP-1 secretion using STC-1 (intestinal endocrine L cells) and NIT-1 (islet β cells). In a co-culture system, C3G treatment increased GLP-1 secretion in STC-1 cells, promoting insulin release in NIT-1 cells under high glucose. Mechanistically, C3G activated the PPARβ/δ-β-catenin-TCF-4 pathway in STC-1 cells, enhancing PG precursor transcription and GLP-1 synthesis.Inhibiting PPARβ/δ with GSK0660 blocked this C3G-induced upregulation. Overall, C3G stimulates GLP-1 secretion from intestinal L cells via this pathway, indirectly boosting insulin release from β cells. These findings enhance T2DM mechanism understanding and suggest the potential of C3G in GLP-1-based T2DM therapy.
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EUROPEAN JOURNAL OF PHARMACOLOGY
·
2025
·
IF 4.7
Xingfang Zhang,Liang Gao,Jianv Wang,Wei Zhang,Dong Xu,Yanhua Wang,Tianlong Liu,Kai Gao,Zhen Ren,Yi Ding
β-Bisabolene, a bioactive sesquiterpene extracted from myrrh—a substance utilized for ischemic stroke treatment—has known therapeutic potential. Nevertheless, the exact mechanisms underlying its beneficial effects on ischemic stroke, along with its potential targets, remain to be fully elucidated. This study aimed to investigate the therapeutic potential of β-Bisabolene in ameliorating ischemic stroke, identify its potential targets and interactions, and explore the underlying pathways involved. Bioinformatics analyses, including network pharmacology and analysis of GEO transcriptomic datasets, were performed to predict the potential targets and mechanisms of β-Bisabolene in ischemic stroke treatment. Preliminary computational validation was achieved through molecular similarity comparisons, molecular docking studies, and molecular dynamics simulations. In vitro experiments were then conducted to confirm the protective effects of β-Bisabolene on microglia injured by oxygen‒glucose deprivation and to validate the findings from the bioinformatics analyses. The results revealed that β-Bisabolene reduces the levels of the proinflammatory cytokines TNF-α, IL-1β and NO; decreases reactive oxygen species levels; and decreases the expression of COX-2, P38, ERK and Keap1. Moreover, it increases the expression of Arg-1, Nrf2, NQO1 and HO-1. These effects are associated with improved survival rates of oxygen–glucose deprivation–damaged microglia. In conclusion, β-Bisabolene may exert anti-inflammatory and antioxidant effects to ameliorate microglial injury induced by ischemic stroke by antagonizing COX-2 and mediating the MAPK signaling pathway and Keap1/Nrf2 pathway. This study provides valuable insights into the therapeutic potential of β-Bisabolene for the treatment of ischemic stroke.
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Food Bioscience
·
2025
·
IF 5.9
Chen Yang,Tianlai Guo,Zizhen Zhang,Aitong Li,Yaqian Han,Yening Qiao,Guoqiang Xia,Lina Wang,Jianming Wang
Oleogels have been extensively employed for the encapsulation and protection of lipophilic nutrient. This study was investigated the effect of low ionic strength (0-100 mM) on the delivery system properties of oleogels prepared by emulsion template method using Soy protein isolate - Xanthan gum nanoparticles (SXNPs) as stabilizers. With the increase of ionic strength (0-100 mM), the particle size of SXNPs showed a trend of decreasing and then increasing. At ionic strength of 50 mM, SXNPs had the best interfacial adsorption properties (82.71 ± 0.19%), which was attributed to the salt ions improved the surface hydrophobicity of SXNPs. Under this condition, the emulsion stabilized by SXNPs exhibited the smallest particle size (2.23 μm) and the best rheological properties. Microscopic images showed that the oleogel had the densest network structure at ionic strength of 50 mM , which enhanced the retention of β-carotene in storage environments at 4, 25 and 55 °C and oil binding capacity of oleogel (10.20%). In vitro digestion showed up to 55.17% free fatty acid release from the oleogels compared to bulk oil, highlighting their potential for controlled nutrient delivery.
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Foods
·
2025
·Vol. 14
·期 9
·1648
·
IF 5.1
Feng Yang,Xin Qi,Yan Chen,Kai Tang,Mengyang Fang,Yanwei Song,Jiufen Liu,Lianming Zhang
In this study, β-cyclodextrin (β-CD) functionalized graphene quantum dots (GQDs) was employed to augment the array of chiral recognition sites, thereby enhancing the affinity of GQDs/β-CD composite for imprinting molecules and realizing heightened chiral selectivity. The incorporation of GQDs/β-CD into the synthesis of molecularly imprinted polymers (MIPs), synergizing with the host-guest inclusion properties of β-CD and the abundant carboxyl groups of GQDs, enhanced the chiral recognition capacity of MIPs materials. Consequently, a novel MIPs/(GQDs/β-CD) sensor with chiral recognition capabilities tailored for D-carnitine was successfully fabricated. The binding mechanism between GQDs/β-CD and D-carnitine was elucidated via Ultraviolet-visible spectroscopy and Fourier transform infrared spectroscopy. The variation in the response signal (ΔI) of the probe molecule exhibited a linear correlation with the logarithm of D-carnitine concentration (lgC) in the range of 1.0 × 10−12mol/L to 1.0 × 10−9mol/L, and the detection limit (3δ/S) was calculated as 2.35 × 10−13mol/L. These results underscore a 7.15-fold enhancement in the selectivity of MIPs/(GQDs/β-CD) sensor for D-carnitine recognition. Moreover, the sensor presented commendable efficacy in real-world scenarios, yielding recovery rates ranging from 98.5% to 103.0% during the determination of D-carnitine content in real samples.
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Environmental Science-Processes & Impacts
·
2025
·
IF 3.9
Xiaomin Ren,Jianying Wang,Fenqing Zhao,Pingping Zhang,Huan He,Zhixiang Xu,Bin Huang,Xuejun Pan
Previous studies have indicated that 6:2 chlorinated polyfluoroalkyl ether sulfonate (6:2 Cl-PFAES), a substitute for perfluorooctane sulfonate (PFOS), caused disruptions in glucose metabolism, but its toxicological mechanisms remain unclear. Pancreatic β cells are responsible for regulating glucose metabolism. This study used mouse insulinoma β cells (β-TC-6) to study the toxic effects of 6:2 Cl-PFAES and elucidate its potential mechanisms. Through cell counting kit-8, trypan blue staining, and apoptosis assays, we found that 6:2 Cl-PFAES showed toxicity to β-TC-6 cells, even surpassing that of PFOS. Mechanistic studies demonstrated that 6:2 Cl-PFAES induced oxidative stress by ascendant levels of reactive oxygen species (ROS) and malondialdehyde (MDA), alongside increased superoxide dismutase (SOD) activity. The oxidative stress-inducing capability of 6:2 Cl-PFAES was stronger than PFOS. Co-exposure with N-acetyl-L-cysteine (NAC), as the ROS scavenger, which obviously reduced the degree of cell apoptosis and the production of ROS, highlighting oxidative stress as a crucial mechanism of 6:2 Cl-PFAES-induced cell death. Transcriptomic analysis further revealed that 6:2 Cl-PFAES affected genes association with the oxidative phosphorylation pathway, confirming the oxidative stress effect, and altered diabetes-related genes expression, suggesting a heightened risk of diabetes. This study provides insights into the toxicity of 6:2 Cl-PFAES on β cells and its potential risks on glucose metabolism disruption.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Lichuang Cao,Ran Chen,Xin Huang,Shuifeng Li,Sufang Zhang,Xiangpeng Yang,Zongmin Qin,Wei Kong,Wei Xie,Yuhuan Liu
In this study, a Petri-dish-based double-layer high-throughput screening method was established to improve glucose tolerance of β-glucosidase Bgl15. Two beneficial mutations were identified, and the joint mutant 2R1 improved the half-maximal inhibitory concentration of glucose from 0.04 to 2.1 M. The crystal structure of 2R1 was subsequently determined at 2.7 Å. Structure analysis revealed that enhancement of glucose tolerance may be due to improved transglycosylation activity made possible by a hydrophobic binding site for glucose as an acceptor and more stringent control of a putative water channel. To further ameliorate the application potential of the enzyme, it was engineered to increase the half-life at 50 °C from 0.8 h (Bgl15) to 180 h (mutant 5R1). Furthermore, supplementation of 5R1 to the cellulase cocktail significantly improved glucose production from pretreated sugar cane bagasse by 38%. Consequently, this study provided an efficient approach to enhance glucose tolerance and generated a promising catalyst for cellulose saccharification.
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PHYSIOLOGY & BEHAVIOR
·
2017
·
IF 2.341
Xiaoyu Song,Bo Liu,Lingyu Cui,Biao Zhou,Weiwei Liu,Fanxing Xu,Toshihiko Hayashi,Shunji Hattori,Yuko Ushiki-Kaku,Shin-ichi Tashiro,Takashi Ikejima
Depression is one of the most frequent psychiatric disorders of Alzheimer's disease (AD). Depression and anxiety are associated with increased risk of developing AD. Silibinin , a flavonoid derived from milk thistle ( Silybum marianum ), has been used as a hepato-protectant in the clinical treatment of liver diseases. In this study, the effect of silibinin on Aβ-induced anxiety/depression-like behaviors in rats was investigated. Silibinin significantly attenuated anxiety/depression-like behaviors caused by Aβ1-42-treatment as shown in tail suspension test (TST), elevated plus maze (EPM) and forced swimming tests (FST). Moreover, silibinin was able to attenuate the neuronal damage in the hippocampus of Aβ1-42-injected rats. Silibinin-treatment up-regulated the function through BDNF/TrkB pathway and attenuated autophagy in the hippocampus. Our study provides a new insight into the protective effects of silibinin in the treatment of anxiety/depression.
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