Journal of Animal Science and Biotechnology
·
2024
·Vol. 15
·期 1
·1-20
·
IF 6.3
Li Daojie,Cai Hongying,Liu Guohua,Han Yunsheng,Qiu Kai,Liu Weiwei,Meng Kun,Yang Peilong
Background Fatty liver hemorrhage syndrome (FLHS) becomes one of the most major factors resulting in the laying hen death for caged egg production. This study aimed to investigate the therapeutic effects of Lactiplantibacillus plantarum ( Lp. plantarum ) FRT4 on FLHS model in laying hen with a focus on liver lipid metabolism, and gut microbiota.
Results The FLHS model of laying hens was established by feeding a high-energy low-protein (HELP) diet, and the treatment groups were fed a HELP diet supplemented with differential proportions of Lp. plantarum FRT4. The results indicated that Lp. plantarum FRT4 increased laying rate, and reduced the liver lipid accumulation by regulating lipid metabolism (lipid synthesis and transport) and improving the gut microbiota composition. Moreover, Lp. plantarum FRT4 regulated the liver glycerophospholipid metabolism. Meanwhile, “gut-liver” axis analysis showed that there was a correlation between gut microbiota and lipid metabolites.
Conclusions The results indicated that Lp. plantarum FRT4 improved the laying performance and alleviated FLHS in HELP diet-induced laying hens through regulating “gut-liver” axis. Our findings reveal that glycerophospholipid metabolism could be the underlying mechanism for the anti-FLHS effect of Lp. plantarum FRT4 and for future use of Lp. plantarum FRT4 as an excellent additive for the prevention and mitigation of FLHS in laying hens.
✓ 已验证
DOI
PubMed
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2018
·Vol. 37
·期 9
·841-850
·
IF 2.016
Yang Lin-Can,Han Li,Ma Hong-Wei,Liu Pi-Bo,Shen He-Yu,Li Chao,Zhang Song-Bo,Li Yang
“Thiol-yne” click reaction has already been widely applied in synthesis and modification of new polymer structures or novel materials due to its specific features. However, in most studies, only chain-end strategy was employed when using the di-addition feature of thiol-yne reaction, thus the in-chain di-addition strategy could endow us with a broader space to develop the synthesis of advanced polymers. Therefore, in this paper, the features of in-chain mono- and di-addition were investigated when modifying the alkyne-functionalized polymers to prepare grafted polymers via thiol-yne click reaction. The results showed that it is almost impossible to obtain the in-chain di-adducts even under excess feeding of chain-end thiol-functionalized grafts, while only the in-chain mono-adducts could be obtained efficiently. Further researches investigated that the controlled grafting could be encountered when carrying out the thiol-yne click reaction between chain-end alkyne-functionalized polystyrenes and chain-end thiol-functionalized polystyrenes under proper feedings. Therefore, the effect of steric-hindrance might be the primary reason for the alternative grafting via thiol-yne click reaction between in-chain and chain-end alkyne-functionalized polymers.
✓ 已验证
DOI
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-11
·
IF 14.7
Zhou Xuefan,Zhang Jun,Luo Hang,Zhang Yan,Tang Shiyu,Huang Houbing,Yuan Xi,Song Miao,Qi He,Zhang Dou
The trade-off between electrostrain and strain hysteresis for piezo/ferroelectric materials largely restrains the development of high precision actuators and remains unresolved over the past few decades. Here, a simple composition of (Bi 0.5 Na 0.5 ) 1- x /100 Sr x /100 TiO 3 in the ergodic relaxor state is collaboratively designed through the segregated domain structure with the ferroelectric core, local polarization heterogeneity, and defect engineering. The ferroelectric core can act as a seed to facilitate the field-induced nonpolar-to-polar transition. Together with the internal bias field caused by defect dipoles and adjusted through electric field cycling and heat treatment technology, a giant unipolar strain of 1.03% is achieved in the x = 30 ceramic with a low hysteresis of 27%, while the electric-field-independent large-signal piezoelectric strain coefficient of ~1000 pm/V and ultralow hysteresis of <10% can be obtained in the x = 35 ceramic. Intriguingly, the low-hysteresis high strain also exhibits near-zero remnant strain, excellent temperature and cycling stability.
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2022
·Vol. 12
·期 1
·1-8
·
IF 4.996
Weng Yue,Su Chun-Jen,Jiang Haoyang,Chiang Chien-Wei
Site-selectively chemical bioconjugation of peptides and proteins can improve the therapeutic exploration of modified protein drugs. Only 3.8% natural abundance of phenylalanine in protein and nearly 90% of proteins contain at least one phenylalanine residue in their sequenced, showing the potential in biopharmaceutical utility of the phenylalanine bioconjugation. However, the covalent bioconjugation of native phenylalanine is one of the most challenging problems in protein modification. Herein, an approach to protein modification is described that relies on a photoredox method for the site-selective bioconjugation of phenylalanine. This methodology has been validated on peptides as well as protein insulin using a straightforward and mild condition. In addition, based on characterization by near-UV CD spectroscopy and small angle X-ray scattering (SAXS), this pyrazole labeling approach permitted the insulin hexamer to completely dissociate into the monomeric form, thus making it a potential candidate for use as rapid-acting insulin for the treatment of diabetes.
✓ 已验证
DOI
高影响因子
CHEMICAL PAPERS
·
2022
·Vol. 76
·期 4
·2145-2152
·
IF 2.146
Zhu Xingji,Kou Xinhui
We disclose a metal-free catalyst system of organic bases/protic acids for effective fully alternating copolymerization of cyclohexene oxide and phthalic anhydride. A series of commercial organic bases and protic acids were employed as binary catalysts, and the effects of different kinds and ratios of organic bases and protic acids were performed to find optimal conditions for achieving the highest catalytic performance. And our results indicated that DMAP/DPP showed higher activity (TOF = 574 h −1 at 140 °C) and perfectly alternating selectivity (ester > 99%), producing polymers with narrow polydispersity (Ð = 1.08 ~ 1.22). Moreover, analysis of the resultant polymers was confirmed by GPC, NMR spectra and MALDI-TOF MS techniques which revealed that P(PA- alt -CHO) polyester with well-defined alternating structure without formation of polyether and DMAP can act as an initiator in DMAP/DPP catalyst system, resulting more effective ring-opening alternating copolymerization. However, trace amount of phthalic acid as an impurity in the system also acted as a bifunctional initiator influencing the molecular weight of the polymer. In addition, the DMAP/DPP catalytic system remained stable at high temperature and the development of this simple catalytic process provided a new chance to exploit in organocatalyzed polymerization.
✓ 已验证
DOI
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2016
·Vol. 18
·期 9
·1-10
·
IF 2.101
Zhong Benbin,Zu Xihong,Yi Guobin,Huang Hailiang,Zhang Minghai,Luo Hongsheng
In this paper, well-ordered Au nanoparticle arrays on silicon substrates were employed as efficient metal-enhanced fluorescence (MEF) substrates for investigating the fluorescence properties of the conjugated polymer poly(3-hexylthiophene) (P3HT). The ordered Au nanoparticle arrays were fabricated by block copolymer self-assembly technology, and the particle sizes were controlled by adjusting the molar ratios of HAuCl 4 precursor to vinyl pyridine units. The approach is economical and suitable to fabricate large-area MEF substrates. The results about fluorescence properties of P3HT showed that the fluorescence intensities of the P3HT films were improved on ordered Au nanoparticle arrays compared to those on bare silicon substrate and were significantly enhanced with the Au nanoparticle sizes increasing. The mechanism is based on localized surface plasmon resonances, coupling and propagating surface plasmons, and the emission enhancement mainly resulted from the increase of the excitation rate. This work provides a new way to prepare efficient MEF substrates for high-performance fluorescence-based devices.
✓ 已验证
DOI
高影响因子
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
·
2016
·
IF 3.031
Xiaobo Xie,Zhenpeng Wang,Yafeng Li,Lingpeng Zhan,Zongxiu Nie
A liquid sampling-atmospheric pressure afterglow microplasma ionization (LS-APAG) source is presented for the first time, which is embedded with both electrospray ionization (ESI) and atmospheric pressure afterglow microplasma ionization (APAG) techniques. This ion source is capable of analyzing compounds with diverse molecule weights and polarities. An unseparated mixture sample was detected as a proof-of-concept, giving complementary information (both polarities and non-polarities) with the two ionization modes. It should also be noted that molecular mass can be quickly identified by ESI with clean and simple spectra, while the structure can be directly studied using APAG with in-source oxidation. The ionization/oxidation mechanism and applications of the LS-APAG source have been further explored in the analysis of nonpolar alkanes and unsaturated fatty acids/esters. A unique [M + O – 3H]+ was observed in the case of individual alkanes (C5–C19) and complex hydrocarbons mixture under optimized conditions. Moreover, branched alkanes generated significant in-source fragments, which could be further applied to the discrimination of isomeric alkanes. The technique also facilitates facile determination of double bond positions in unsaturated fatty acids/esters due to diagnostic fragments (the acid/ester-containing aldehyde and acid oxidation products) generated by on-line ozonolysis in APAG mode. Finally, some examples of in situ APAG analysis by gas sampling and surface sampling were given as well.
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 191
·期 1
·1-8
·
IF 5.7
Che Dou,Cao Xitao,Chen Chong,Yan Hui
The rapid detection of Pseudomonas aeruginosa ( P. aeruginosa ) is of great significance for the diagnosis of medical infection. In view of the above, a novel aptasensor based on fluorescence resonance energy transfer (FRET) was developed. It contained aptamer-coupled upconversion nanoparticles (UCNPs-apt) as a donor (excitation 980 nm) and molybdenum disulfide (MoS 2 ) nanosheets as an acceptor. The upconversion fluorescence aptamer system was investigated to obtain the optimal parameters of MoS 2 concentration, the incubation time of UCNPs-apt/MoS 2 and P. aeruginosa , and pH. Based on the optimal parameters, a linear calibration equation (emission 654 nm) with a wide detection range 8.7 × 10 ~ 8.7 × 10 7 cfu/mL, a high coefficient of determination R 2 0.9941, and a low limit of determination (LOD) 15.5 cfu/mL were established. The method was validated with P. aeruginosa infected foci of mouse wound. The advantage of this aptasensor is that analysis results can be obtained within 1.5 h, which was much faster than that of the standard method (18–24 h). Furthermore, combined with a portable instrument, it can be used as a point-of-care testing for the early detection of P. aeruginosa infection, which is useful for selecting the correct antibiotics to achieve good therapeutic effects. Additionally, it also has a broad application prospect in food and environmental areas. Graphical abstract
✓ 已验证
DOI
高影响因子
Journal of Translational Medicine
·
2024
·Vol. 22
·期 1
·1-13
·
IF 6.1
Li Jing,Wang Xiaochen,Bai Jie,Wei Huangzhao,Wang Wenbo,Wang Shuai
Background Hypertension influences the inflammatory pathological changes in the retina. The function of the inflammasomes is significant. To see if Sirtuin 1 (SIRT1) regulates angiotensin II (Ang II)-induced hypertensive retinopathy and inflammation by modulating NOD-like receptor thermal protein domain associated protein 3 (NLRP3) inflammasome activation and the potential protective effects of fucoidan (FO) in mouse retinal vascular endothelial cells (mRECs) and mice retina. Methods The diagnosis of hypertensive retinopathy was made after three weeks of Ang II infusion (3000 ng/kg/min). One day prior to the commencement of Ang II infusion, the mice were treatment with NLRP3 inhibitor MCC950 (10 mg/kg/day, intraperitoneal injections) or FO (300 mg/kg/day, oral gavage). A blood pressure was recorded. Hematoxylin and eosin (H&E) staining was used to conduct pathological alterations, dihydroethidium bromide (DHE) was utilized to assess oxidative stress damage in the retina, and fluorescence angiography was used to identify vascular disorders in the eye. Using immunohistochemical labeling, NLRP3 expression was found. Reactive protein and mRNA expression levels in mouse retina and cells were assessed using Western blot and real-time quantitative polymerase chain reaction (RT-qPCR). Results NLRP3 inflammasome activation and SIRT1 decrease were brought about by Ang II infusion. Retinopathy and dysfunction were lessened by MCC950 target-induced NLRP3 inflammasome activation, while overexpression of SIRT1 had the opposite impact on NLRP3 inflammasome activation, indicating that SIRT1 functions as an upstream regulator of NLRP3 activity. FO may improve SIRT1 expression and decrease NLRP3 activation in retinopathy and dysfunction brought on by Ang II, and the effects were consistent across both in vivo and in vitro models. Conclusions SIRT1 adversely regulates the NLRP3 inflammasome pathway, which in turn increases Ang II-induced inflammation and hypertensive retinopathy. FO may mitigate Ang II-induced retinopathy and dysfunction via modulating the expression of SIRT1/NLRP3. This implies practical approaches to the management of hypertensive retinopathy.
✓ 已验证
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2024
·
IF 3.5
He Taotao,Wang Fei,Feng Junfeng,Liu Yancong,Chen Yuwei,Guo Feihong,Liu Peng,Jiang Xiaoxiang,Jiang Jianchun
To efficiently convert the microalgae to valuable and renewable fuels, the microalgae oil was first extracted and then transformed into the second-generation biodiesel. Among all solvents that were used for the microalgae oil extraction, the polar single solvent system methanol achieved the optimal value (47.8% extraction yield) because the polar cell membrane was easily dissolved by the polar solvent. Hexadecanoic acid (38.54%) and docosanoic acid (54.39%) dominated in fatty acid of microalgae oil. In the catalytic deoxygenation of microalgae oil, the Co-Al nanosheet displayed a higher performance than Co/Al 2 O 3 because of its high Co content and lamellar structure, exposing massive Co sites with high dispersion and small particle size. Moreover, when the reaction temperature was raised to 240 °C, the 100% conversion and 100% alkane yield were obtained, and the further temperature rise in temperature led to the cracking reaction. Graphical Abstract
✓ 已验证
DOI
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2023
·Vol. 30
·期 8
·1-7
·
IF 2.8
Wu Fang,Pu Zejun,Hou Hongbo,Li Xianyong,Zhu Rongli,Wang Xu,Yu DaYang,Zhong Jiachun
Bioderived polycarbonate (ISB-PC) was synthesized via transesterification and polycondensation of isosorbide (ISB) and diphenyl carbonate (DPC) using lithium acetoacetone as a catalyst. The resulting ISB-PC exhibited high glass transition temperatures similar to bisphenol A polycarbonate (BPA-PC) and was thermally stable up to 350 ℃. The incorporation of isosorbitol into the polymer chain imparted an improved mechanical property of ISB-PC, such as a higher bending and tensile modulus and higher elongation at break. ISB-PC can be readily cast into transparent films with a tensile strength of 73.3 MPa and a tensile modulus of 534 MPa. ISB-PC displayed high UV transmittance and possessed blue fluorescence under UV irradiation. At the same time, ISB-PC also has strong anti-yellowing ability.
✓ 已验证
DOI
高影响因子
BIOTECHNOLOGY LETTERS
·
2023
·Vol. 45
·期 10
·1355-1364
·
IF 2.7
Gao Yahui,Li Fen,Wang Yulu,Chen Zhou,Li Zijie
Purpose In our previous study, we constructed a one-pot multi-enzyme system for rare ketoses synthesis based on L-rhamnulose-1-phosphate aldolase (RhaD) from accessible glycerol in vitro. To eliminate tedious purification of enzymes, a facile Escherichia coli whole-cell cascade platform was established in this study. Methods To enhance the conversion rate, the reaction conditions, substrate concentrations and expressions of related enzymes were extensively optimized. Results The biosynthetic route for the cascade synthesis of rare ketoses in whole cells was successfully constructed and three rare ketoses including D-allulose, D-sorbose and L-fructose were produced using glycerol and D/L-glyceraldehyde (GA). Under optimized conditions, the conversion rates of rare ketoses were 85.0% and 93.0% using D-GA and L-GA as the receptor, respectively. Furthermore, alditol oxidase (AldO) was introduced to the whole-cell system to generate D-GA from glycerol, and the total production yield of D-sorbose and D-allulose was 8.2 g l −1 only from the sole carbon source glycerol. Conclusion This study demonstrates a feasible and cost-efficient method for rare sugars synthesis and can also be applied to the green synthesis of other value-added chemicals from glycerol.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）