JOURNAL OF SEPARATION SCIENCE
·
2022
·Vol. 45
·期 11
·1847-1855
·
IF 3.614
Ben Chen,Haibo You,Liqun Fang,Tingting Lin,Ping Xu,Chu Chu,Shengqiang Tong
Analytical enantioseparations of five N-alkyl drugs, fluoxetine hydrochloride, labetalol, venlafaxine hydrochloride, trans -paroxol, and atropine sulfate, were investigated by reverse phase high-performance liquid chromatography with sulfobutylether-β-cyclodextrin as chiral mobile phase additive. Effects of various factors such as composition of mobile phase, concentration of cyclodextrins, and column temperature on retention and enantioselectivity were studied. Apparent formation constant between methanol, acetonitrile, and sulfobutylether-β-cyclodextrin were determined to be 2.90 × 10 −3 and 1.00 × 10 −4 L mmol −1 under 25°C using UV-spectrophotometry. Van't Hoff plots were used to investigate thermodynamic parameters for enantiomers–stationary phase interaction and formation of inclusion complex. Two retention models were employed individually for evaluation of inclusion complexation between five racemates and sulfobutylether-β-cyclodextrin. The second model with complex adsorption was more accord with the retention behavior of fluoxetine hydrochloride, labetalol, and venlafaxine hydrochloride enantiomers, while the first model was more consistent with the retention behaviors of trans -paroxol and atropine sulfate. In the selected mobile phase, stoichiometric ratio for both of inclusion complex was found to be 1:1.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2016
·
IF 2.687
Hongcheng Liu,Tao Lin,Xianglei Cheng,Ning Li,Luxiang Wang,Qiwan Li
A simple, accurate and highly sensitive multiresidues method was developed for the determination of 9 anabolic steroids and 16 β-agonists in milk. Target compounds were extracted and separated by a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) method with Primary secondary amine (PSA) and Zinc oxide (ZnO) nanoparticles as adsorbents. The analytes were determined by ultra high performance liquid chromatography coupled to an electro-spray ionization tandem mass spectrometer (UHPLC–MS/MS) operating in negative/positive multiple reaction monitoring mode. The effect of QuEChERS including organic solvent, amount of ZnO nanoparticle was evaluated. Good linearity was obtained for the analytes in the concentration range of 0.1–200 μg/L with a correlation coefficients higher than 0.994. Decision limits (CCα) was from 0.007 μg/kg–0.1 μg/kg, and detection capabilities (CCβ) was in the range of 0.02–0.4 μg/kg. The recoveries of these compounds were between 63% and 126% at three fortified levels. Reproducibility represented by RSD was with 10% or less. The method was successfully applied to screening of real milk samples obtained from local markets and confirmation of the suspected target analytes.
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Applied Catalysis O: Open
·
2024
·
IF 0
Xue Zhang,Baowei Zhao,Yetong Liu,Min Yao,Yanjuan Li,Nan Wu
Sonochemistry was used to improve the degradation performance of trace antibiotics in water by the adsorption-catalytic oxidation system composed of Nitrogen-doped cyclodextrin reduced graphene oxide (N-β-rGO) and peroxymonosulfate (PMS). The results indicated that after loading ultrasound, >90% of antibiotics such as SMZ, LVX, CLI, TC, PEN, and CHL were removed within 10 min. Increasing the sound intensity, raising the system temperature, and increasing the initial PMS concentration all improved the strengthening effect. Among them, the removal rate was enhanced by >10% under low pH conditions. Moreover, the calculated enhancement factor of ultrasound enhancement was >1.30. The magnitude of the enhancement effect was correlated with the logP of antibiotics.
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Scientific Reports
·
2019
·Vol. 9
·期 1
·1-12
·
IF 4.011
Li Xingqiang,Zhang Bingyu,Zhao Wei,Yang Shanshan,Yang Xinjuan,Zhou Le
Thirty new title compounds along with five known analogues were prepared from commercially available 2-arylhydrazin-1-ium chlorides and α-ketoglutaric acid. The mycelium growth rate method was used to evaluate inhibition activity against six strains of plant pathogenic fungi. Most of the compounds displayed the activity for each the fungi at 150 μΜ, higher than azoxystrobin, a positive drug. Compound 6-2 showed the lowest average IC 50 value of 4.58 μg/mL for all the fungi where F . solani exhibited the highest susceptibility to most of the compounds. For F . solani , some compounds were more active with IC 50 values of 2.67–8.48 μM than thiabendazole (IC 50 = 9.30 μM) and/or carbendazim (IC 50 = 3.36 μM). The SAR showed that the activity is significantly affected by substituents on the A-ring and/or D-ring along with the degree of unsaturation of the C-ring. Thus, a series of new β-carboline compounds with potent antifungal potential were found.
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FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Yinyue Yang,Ming Zhang,Junhua Li,Yujie Su,Luping Gu,Yanjun Yang,Cuihua Chang
This study explored the construction of egg white protein particles (EWPP) and/or rhamnolipid (Rha) based emulsion gels with β-sitosterol (Sit) as gelation factor. The properties of the emulsion gels and the application in cookies were investigated to further reveal the functional mechanism of each component. The rheological and microstructure results of the emulsions showed that emulsion gels had suitable plastic texture with lower melting rate and faster cooling rate compared with butter. EWPP stabilized the droplet while Rha reduced the droplet size and enhanced the fluidity of the emulsion gels. In cookie system, Sit in the emulsion gels provided the appropriate hardness and uniform matrix with emulsifiers. EWPP and Rha effectively improved the appearance, texture and mouthfeel of cookies and extended cookies’ frangibility during storage. The constructed composite emulsion gels showed promising potential as plastic fat substitutes, to be used in healthy bakery systems to decrease saturated fatty acids.
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POLYMERS FOR ADVANCED TECHNOLOGIES
·
2021
·Vol. 33
·期 2
·492-504
·
IF 3.665
Zhongyang Yao,Qiming Cao,Chenyang Li,Weiguang Gong,Xin Meng
In this article, a β-cyclodextrin(CD)/cardanol (CA) inclusion complex (IC) was fabricated by co-precipitation method, which was used to guarantee excellent thermal oxidative stability and environmental-response antioxidation releasing property of PLA. The results showed that CA was successfully immobilized into β-CD with a stoichiometric ratio of 1:1, and the immobilization inhibited the migrant of CA from PLA during storage, resulting in the enhancement of antioxidant life of CA in PLA. Thus, PLA stabilized by IC (PLA/IC) maintained higher oxidation onset temperature (OOT) and molecular weight after long-term thermal oxidative aging. Moreover, CA releasing comparison from PLA/IC and PLA stabilized by CA (PLA/CA) in food stimulant suggested that PLA/IC released CA faster than PLA/CA when PLA contacted with food stimulants due to the solubilization of β-CD for CA, which would induce the quicker antioxidation of CA in food. In a word, IC can make PLA with longer anti-oxidative stability during storage and quicker environmental-response antioxidation releasing property during usage, showing strong potential as an active food-packaging materials.
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POLYMERS FOR ADVANCED TECHNOLOGIES
·
2022
·Vol. 33
·期 12
·4120-4130
·
IF 3.348
Xue Meng,Da Zhang,Meng Wang,Ronghui Zhao,Pengfei Zhang,Jingyuan Zhao,Kuilin Deng
For the nontraditional aggregation-induced emission (AIE) active polymers, the heterocyclic structure on the macromolecular backbone and side chains play a crucial role in its fluorescence generation. In this paper, we facilely prepared a series of poly(β-lactam) derivatives (PLTM) by Staudinger polymerization. As expected, PLTMs with four-membered heterocycles along the main-chain shows a remarkable AIE performance with the fluorescence emission at 520 nm, while no fluorescence was observed for both β-lactam (repeating unit) and a model polymer for PLTM. The fluorescence properties of PLTMs greatly depend on the intramolecular aggregation of β-lactam structures, the size of the substituents, and appropriate length of CH 2 spacer. In addition, PLTMs can specifically detect Fe 3+ ions with a limit of detection (LOD) of 7.2 μM even in the presence of other 13 metal ions. Similar to the biological activity of β-lactam, AIE-active PLTMs shows a bactericidal effect on Staphylococcus aureus and Escherichia coli with the minimum inhibitory concentration of about 8 and 4 mg/mL. In brief, the preparation and versatility of PLTMs with β-lactam structure provide useful references for designing other nontraditional AIE-active polymers as well as for ion detection and antibacterial processes.
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Food Biophysics
·
2024
·Vol. 19
·期 2
·310-320
·
IF 2.8
Wu Zi-ke,Chen Ru-kang,Shi Yu,Zhu Chen-min,Wang Yi-ran,Shi Yu-gang,Ettelaie Rammile,Gu Qing
Octyl gallate (GAC8) as a bioactive compound has excellent antibacterial effectiveness, but its poor hydrophilicity limits its applications. In this work, GAC8 was encapsulated into hydroxypropyl- β -cyclodextrin (HP β CyD) cavity to form an inclusion complex (GAC8/HP β CyD-IC), and their antibacterial activities were investigated. Phase solubility test suggested that the aqueous solubility of GAC8 was prominently enhanced after forming the inclusion complex. The aqueous solution of GAC8/HP β CyD-IC yielded uniform fiber morphology with ~ 900 nm average fiber diameter. The fabricated GAC8/HP β CyD-IC nanofibers (GAC8/HP β CyD-IC NFs) were characterized by 1 H NMR, FT-IR, XRD, DSC, and TGA, revealing successful synthesis of GAC8/HP β CyD-IC NFs and the thermal stability of GAC8 was enhanced by inclusion complexation with HP β CyD. Furthermore, GAC8/HP β CyD-IC NFs possessed antibacterial activity against E. coli (12.5 mm zone of inhibition), S. aureus (18.5 mm zone of inhibition). The results of DNA and protein leakage in the experiment indicated that GAC8/HP β CyD-IC NFs can disrupt the membrane integrity of bacteria. Meanwhile, GAC8/HP β CyD-IC NFs suppressed the colony growth of E. coli on Chinese giant salamander meat. Overall, the nanofibers encapsulating GAC8/HP β CyD-IC were potential antibacterial food packaging materials.
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Journal of Macromolecular Science Part B-Physics
·
2020
·
IF 0.911
Hailin Xie,Zhihua Zhou,Wenjuan Liu,Yanmin Zhao,Tianlong Huang,Ping Chen,Ziwei Zhou,Dan Wang,Jianglong Duan,Jianjun Fang
Polymeric/ceramic composite scaffolds that are biocompatible and biodegradable are widely used for tissue engineering applications. In this work a series of poly(L-lactide-co-glycolide-co-ε-caprolactone)/nano-biaoactive glass-nano-β-tricalcium phosphate composite scaffolds were successfully fabricated and the influences of the inorganic content and freezing temperature on the physical properties were studied. The composite scaffolds with various inorganic contents showed an interconnected pore structure with irregular shapes. The composite scaffolds had a porosity that was reduced with increasing inorganic content and decreasing freezing temperature. The incorporation of inorganic fillers and decreasing freezing temperature improved the mechanical properties of the hybrid scaffolds. By appropriate control of these two factors (10.0 wt% content of NBAG and β-TCP with freezing at −30 °C) a suitable composite scaffold was prepared as a potential bone tissue engineering implant.
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Environmental Science-Processes & Impacts
·
2025
·
IF 3.9
Xiaomin Ren,Jianying Wang,Fenqing Zhao,Pingping Zhang,Huan He,Zhixiang Xu,Bin Huang,Xuejun Pan
Previous studies have indicated that 6:2 chlorinated polyfluoroalkyl ether sulfonate (6:2 Cl-PFAES), a substitute for perfluorooctane sulfonate (PFOS), caused disruptions in glucose metabolism, but its toxicological mechanisms remain unclear. Pancreatic β cells are responsible for regulating glucose metabolism. This study used mouse insulinoma β cells (β-TC-6) to study the toxic effects of 6:2 Cl-PFAES and elucidate its potential mechanisms. Through cell counting kit-8, trypan blue staining, and apoptosis assays, we found that 6:2 Cl-PFAES showed toxicity to β-TC-6 cells, even surpassing that of PFOS. Mechanistic studies demonstrated that 6:2 Cl-PFAES induced oxidative stress by ascendant levels of reactive oxygen species (ROS) and malondialdehyde (MDA), alongside increased superoxide dismutase (SOD) activity. The oxidative stress-inducing capability of 6:2 Cl-PFAES was stronger than PFOS. Co-exposure with N-acetyl-L-cysteine (NAC), as the ROS scavenger, which obviously reduced the degree of cell apoptosis and the production of ROS, highlighting oxidative stress as a crucial mechanism of 6:2 Cl-PFAES-induced cell death. Transcriptomic analysis further revealed that 6:2 Cl-PFAES affected genes association with the oxidative phosphorylation pathway, confirming the oxidative stress effect, and altered diabetes-related genes expression, suggesting a heightened risk of diabetes. This study provides insights into the toxicity of 6:2 Cl-PFAES on β cells and its potential risks on glucose metabolism disruption.
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产品关联
DOI
PubMed
高影响因子
npj Science of Food
·
2025
·Vol. 9
·期 1
·1-9
·
IF 7.8
Ye Xiang,Chen Wen,Yan Fujie,Zheng Xiaodong,Tu Pengcheng
In late-stage type 2 diabetes mellitus (T2DM), impaired islet β cell function leads to absolute insulin deficiency, thereby disrupting blood glucose homeostasis. GLP-1, an incretin hormone, stimulates insulin secretion from islet β cells post-meals. This study investigated the effects of anthocyanin cyanidin-3-O-glucoside (C3G) on GLP-1 secretion using STC-1 (intestinal endocrine L cells) and NIT-1 (islet β cells). In a co-culture system, C3G treatment increased GLP-1 secretion in STC-1 cells, promoting insulin release in NIT-1 cells under high glucose. Mechanistically, C3G activated the PPARβ/δ-β-catenin-TCF-4 pathway in STC-1 cells, enhancing PG precursor transcription and GLP-1 synthesis.Inhibiting PPARβ/δ with GSK0660 blocked this C3G-induced upregulation. Overall, C3G stimulates GLP-1 secretion from intestinal L cells via this pathway, indirectly boosting insulin release from β cells. These findings enhance T2DM mechanism understanding and suggest the potential of C3G in GLP-1-based T2DM therapy.
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PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2025
·
IF 3.5
Yongjun Tang,Zhengning Feng,Chenlu Ma,Nian Jang,Xiaolong Chen,Yingxu He,Francis L. Martin,Hui Liu,Weiyi Pang
Breast cancer has a high global incidence, and benzo[ a ]pyrene (B[ a ]P) is considered a contributing factor that increases carcinogenic risk. This study examined B[ a ]P's oncogenic mechanisms in mammary epithelial cells. Chronic B[ a ]P exposure induced morphological changes and enhanced proliferative/clonogenic capacity in MCF-10A cells. Chronic B[ a ]P exposure altered gene expression in MCF-10A cells, revealing differential levels of circRNAs, lncRNAs, miRNAs, and mRNAs. qRT-PCR validation demonstrated strong alignment with RNA-seq results, ensuring sequencing reliability. Additionally, chronic B[ a ]P exposure upregulated the protein expression of AhR and ARNT, as well as TGF- β , pSmad2/3, and KRT14 , while increasing Vimentin expression and decreasing E-cadherin expression. Notably, treatment with the TGF- β inhibitor SB431542 reversed these protein expression changes in transformed cells. These results show that exposure to Chronic B[ a ]P induces MCF-10A cell transformation. The underlying mechanisms involve significant transcriptional alterations, AhR/ARNT expression regulation, TGF- β signaling pathway activation, KRT14 protein modulation, and EMT. Furthermore, Chronic B[ a ]P exposure may drive transformation through TGF- β modulation. Chronic B[ a ]P exposure promotes breast carcinogenesis, revealing mechanistic insights and potential preventive biomarkers.
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DOI
PubMed
高影响因子
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