PLANT MOLECULAR BIOLOGY REPORTER
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2025
·Vol. 44
·期 1
·21
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IF 1.4
Fan Huiyan,Zhou Jingzhi,Ying Yiling,Tang Kaijie,Han Jiayi,Wang Qiuying,Kai Guoyin
Background (TF) R2R3-MYB transcription factors (TFs) play crucial roles in regulating plant secondary metabolism, which is vital for growth, development, and stress responses. Salvia miltiorrhiza , a medicinal plant, is known for its bioactive compounds such as salvianolic acids. Understanding the R2R3-MYB TF family in S. miltiorrhiza could provide insights into the regulation of secondary metabolism and contribute to quality improvement in this important medicinal plant. Methods and Results We conducted a comprehensive analysis of the R2R3-MYB TF family in S. miltiorrhiza , identifying 431 MYB-like TFs, including 149 R2R3-MYB TFs. Phylogenetic analysis classified these TFs into 27 distinct groups. Chromosomal localization analysis revealed their widespread distribution across all chromosomes. Structural analysis showed that most R2R3-SmMYB genes contain untranslated regions (UTRs) and conserved motifs, with motif5 potentially playing a key role in differentiation. Interspecific collinearity analysis demonstrated the highest collinearity between S. miltiorrhiza and Salvia baicalensis , with 12 intraspecific collinear genes exhibiting interspecific collinearity with multiple species. Promoter analysis identified abundant light- and hormone-responsive elements, particularly MeJA-responsive elements. Expression profiling highlighted SmMYB50 as highly responsive to MeJA, ABA, and YE induction, promoting salvianolic acid accumulation. Transgenic validation confirmed that SmMYB50 activates key biosynthetic genes such as SmCYP98A14 , crucial for SalB synthesis. Conclusions Our findings provide valuable insights into the functional roles of R2R3-SmMYB-TFs in regulating secondary metabolism in S. miltiorrhiza . SmMYB50 was identified as a key regulator, offering new directions for future research on metabolic pathways and quality improvement in this important medicinal plant.
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IONICS
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2025
·
IF 2.6
Huang Xiaoyu,Zhang Yaowen,Yang Jian,Xiao Zhizhong,Lu Huidan,Liu Yongping
The synergistic modulation of both the surface electronic configuration and micromorphology of the catalyst is crucial for its electrochemical hydrogen evolution activity. This research successfully developed an efficient Ru-WS 2 NS/CC electrocatalyst for the hydrogen evolution reaction (HER) using a simple two-step strategy involving a solvothermal reaction followed by chemical vapor deposition. The catalyst exhibits excellent electrocatalytic activity and remarkable stability in acidic electrolyte, demonstrating an overpotential of 128.8 mV (at 10 mA cm − 2 ), a Tafel slope of 58.7 mV dec − 1 , and a low charge transfer resistance of only 3.8 Ω, while maintaining high catalytic activity for at least 24 h under acidic conditions. The enhanced HER performance of the Ru-WS 2 NS/CC catalyst is attributed to two main factors: first, the strong electronic interaction induced by Ru doping, which improves the intrinsic electrocatalytic activity; second, the uniform and thin nanosheet morphology of WS 2 provides a highly developed surface area that facilitates hydrogen adsorption and desorption. Density functional theory (DFT) simulations further indicate that introducing Ruthenium (Ru) dopants substantially lowers the Gibbs free energy (ΔG H* ) associated with hydrogen adsorption on the catalyst. Analysis of charge density differences corroborates that such doping strategy efficiently tailors the electronic configuration of the WS 2 support, thereby optimizing its interfacial characteristics. This work proposes an effective electronic structure modulation strategy for developing three-dimensional materials into high-efficiency hydrogen evolution electrocatalysts.
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Food Analytical Methods
·
2025
·Vol. 19
·期 1
·30
·
IF 3
Yin Yitong,Wang Xin,Hao Huihui,Zhao Xiaolei,He Jinxing
A core–shell structured magnetic molecularly imprinted nanoparticle (MMINP) was developed, capable of forming a photonic crystal (PC) sensor in the presence of a magnetic field for the detection of sulfamethazine (SM2) residues in food. The preparation conditions were meticulously optimized, and the adsorption performance of MMINPs was comprehensively characterized through Adsorption Kinetics Experiments and Equilibrium Binding Experiments. As the concentration of SM2 varied, the structural colors were systematically characterized under a designated magnetic field strength. The experiments revealed that within the range of SM2 concentrations from 10 −1 μg/L to 10 3 μg/L, there was a shift in the reflected wavelength from 570 to 610 nm, and the color changed from green to red–orange. The sensor achieved a minimum detection limit of 2.75 μg/L, a maximum adsorption capacity of 3.86 mg/g, an imprint factor of 1.70, and was reused for at least five adsorption-resolution cycles. The recoveries ranged from 69.97% to 102.68% and RSD < 8.73%. The sensor introduces an innovative approach for the rapid visual colorimetric detection of SM2 in complex food matrices.
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ARCHIVES OF MICROBIOLOGY
·
2025
·Vol. 208
·期 1
·1
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IF 2.6
Zhong Huichang,Chen Liyi,Liu Weifeng,Tao Yong
Xanthophyllomyces dendrorhous (anamorph form Phaffia rhodozyma ) is a key microbial host for industrial astaxanthin production, where yield depends on both the efficiency of the biosynthetic pathway and overall metabolic robustness. To develop superior industrial strains, we established an efficient evolutionary-enrichment system and an accelerated evolutionary workflow that couples astaxanthin production with cell growth under optimized oxidative stress conditions. This strategy enabled the enrichment of mutants exhibiting both high astaxanthin production and strong industrial fitness. Using this approach, we employed a high astaxanthin-producing X. dendrorhous strain A06 as the parental strain for evolution. After several rounds of screening over 2,000 colonies, we successfully identified the top-performing mutant, TP-4. TP-4 demonstrated a stable 20% increase in astaxanthin production relative to the parental strain, along with faster growth and enhanced fermentation performance. The genome of TP-4 was sequenced, and comparative genomic analysis against the parent strain A06 and a low-astaxanthin-producing control strain, C01, revealed multiple mutations potentially underlying its superior phenotype. These findings provide new insights into the metabolic and genetic basis of astaxanthin overproduction. This work establishes an efficient evolutionary platform for strain improvement and provides a mechanistic understanding of astaxanthin overproduction in X. dendrorhous , laying the groundwork for further optimization and industrial-scale application.
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高影响因子
CELLULOSE
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2025
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IF 4.8
Zheng Mengjie,Guo Qingfeng,Yue Yizhi,Lu Chaoyang,Ran Jianhua,Bi Shuguang
A multifunctional cotton fabric was fabricated by in-situ synthesis of zeolitic imidazolate framework-8 (ZIF-8) on a polydopamine (PDA)-modified cotton substrate. Leveraging the reducing capability of PDA, silver nanoparticles (Ag NPs) were simultaneously incorporated into and around the ZIF-8 nanocrystals. The successful fabrication of the Ag/ZIF-8 functional cotton fabric was confirmed by characterization techniques including SEM, XRD, XPS, and TG. The modified fabric exhibits exceptional photocatalytic performance and cycling stability, achieving 93.4% degradation of methylene blue (MB) under visible light irradiation within 170 min and retaining 90.2% efficiency after three degradation cycles. Furthermore, the synergistic effect between Ag and ZIF-8 endows the fabric with significant antibacterial activity and efficient heavy metal ion adsorption capacity. Therefore, this study provides a novel strategy for designing multifunctional materials, holding significant promise for advancing the development of multifunctional textiles.
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Nature Communications
·
2025
·
IF 15.7
Yao Yuan,Zhang Xinyue,Cao Yingying,Liu Long,Zhang Yanqiang,Zhang Suojiang
Cyclopropane-based aerospace fuels exhibit the excellent volumetric calorific value, leading to broad application prospects in aircrafts. However, due to the highly rigid structure of the cyclopropane (compared to cyclobutane or cyclopentane, etc.) and the difficulty in forming metal-carbene intermediates, there are still significant challenges in the precise synthesis of multi-cyclopropane-based high-energy fuels ( ≥ 2 cyclopropane structures). Herein, via the introduction of bending strain on metal organic complex, we report a single-site Cu/single-walled carbon nanotube catalyst (Cu/SWCNT), which possesses 2.6-fold conversion (up to 77.4%) and 4-fold selectivity (up to 45.7%) as that without bending strain for the preparation of bi-cyclopropane-based fuels from dienes. XAFS and DFT calculations show that bending strain enhances the upward shift of d-band center of β electrons (−4.370 to −4.366), thus promotes the adsorption and activation of CH 2 N 2 and alkenes. Moreover, bending strain elongates Cu-O bonds (1.91 to 1.96 Å), making it easier for CH 2 N 2 to insert and form Fischer carbene intermediates. These two aspects obviously reduce the reaction energy barrier from 40.3 to 33.7 kcal·mol -1 for the cyclopropanation process. Our work provides a method of strain engineering to regulate C-C coupling reactions at the molecular level, and achieve highly efficient cyclopropanation from alkenes.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2025
·Vol. 36
·期 36
·2284
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IF 2.8
Danli Yao,Cheng Rong
To address the urgent need for lightweight, wideband (S-C band) microwave absorbing materials for naval ship stealth, this study designed and prepared Fe 3 O 4 /activated carbon (Fe 3 O 4 /AC) composites using a solvothermal method. By controlling the distribution of Fe 3 O 4 nanoparticles within the hierarchical pores of AC, a magneto-dielectric synergistic loss system was constructed. At a Fe 3 O 4 :AC mass ratio of 20:5 (Sample S5), the material demonstrated breakthrough wave absorption performance at a practical thickness of 5.0 mm: a minimum reflection loss (RL) of − 42.63 dB at 6.0 GHz and an effective absorption bandwidth (EAB, RL ≤ − 10 dB) of 3.12 GHz (4.16–7.28 GHz), fully covering the critical S–C band (2–8 GHz) for shipborne radar. This performance enhancement arises from the synergistic effect between enhanced interfacial polarization relaxation due to AC’s hierarchical pore structure and the magnetic loss (dominated by low-frequency natural resonance) from Fe 3 O 4 . This synergy significantly optimizes impedance matching and excites λ/4 interference cancellation. This study provides a lightweight composite with strong absorption and broad bandwidth in the S–C band, offering potential for naval stealth applications.
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JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
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2025
·Vol. 44
·期 1
·321
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IF 12.8
Lei Yu,Bai Yaowei,Bai Xiatong,Sun Bo,Zhu Licheng,Wu Wenlong,Su Yang,Zhang Hongsen,Wang Yingliang,Zheng Chuansheng
Background and aims Radiofrequency ablation (RFA) is an effective treatment for hepatocellular carcinoma (HCC), but incomplete ablation and recurrence of residual tumors remain significant challenges, partly due to local inflammation and elevated COX-2 levels in the tumor microenvironment. This study aims to investigate the potential of combining celecoxib, a COX-2 inhibitor, with anti-PD-1 monoclonal antibody (αPD-1) to enhance anti-tumor efficacy and activate immune responses. Methods In vitro, ELISA was used to assess the effects of radiofrequency heat treatment and celecoxib on PGE 2 secretion by Hepa1-6 cells. Scanning electron microscopy, flow cytometry, and CCK-8 assays were employed to evaluate the function and cytotoxic activity of NK92 cells against Hepa1-6 cells. In vivo, orthotopic HCC mice were divided into five groups to evaluate tumor volume, pathology, and survival. The role of celecoxib in the COX-2/PGE 2 /NK cell axis and its impact on NK cell immune function were investigated. Results In vitro experiments showed that celecoxib reversed PGE 2 -mediated suppression of NK cell function and cytotoxic activity against HCC cells by inhibiting PGE 2 secretion. In vivo, orthotopic HCC mice treated with celecoxib and αPD-1 exhibited significantly reduced tumor volumes, attenuated the infiltration and activation of NK cells, and prolonged survival compared to control groups. The combination therapy also demonstrated a notable abscopal-like effect, inhibiting metastatic tumor growth and activating systemic immunity. Conclusions Taken together, these findings suggest that celecoxib combined with αPD-1 represents a promising strategy for treating residual HCC after RFA, with enhanced anti-tumor effects and good safety. Graphical abstract
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MICROCHIMICA ACTA
·
2025
·Vol. 193
·期 1
·51
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IF 5.3
Li Xiaoqing,Bao Chengqi,Shao Rong,Deng Lei,Zheng Wei,Yang Minghui
An innovative organic-inorganic heterojunction based on meso -tetra(4-carboxyphenyl)-porphine (TCPP) and CsPbBr 3 perovskite is reported. Additionally, cobalt nitride (Co 4 N) was employed as mimetic enzyme of lactic acid oxidase (LOX), thereby enabling sensitive detection of lactic acid in serum through photoelectrochemical (PEC) detection. The stability of CsPbBr 3 was significantly improved in aqueous solutions by substituting some of the long-chain ligands on its surface with TCPP, an organic semiconductor containing π-π bonds and carboxyl groups. Furthermore, the formation of a type-II heterojunction between TCPP and perovskite improved carrier separation and transport capabilities, resulting in more stable and enhanced photocurrent response. The non-enzymatic catalyst, Co 4 N nanoparticles, demonstrate efficient catalytic activity toward lactic acid oxidation and enhance electron transfer to the heterojunction interface, thereby increasing the response current. The prepared PEC biosensor exhibits an effective sensing capability for lactic acid, with a working range of 0.2 mM to 10 mM and a detection limit of 0.03 mM. Furthermore, the biosensor features good selectivity and reproducibility, making it ideal for measuring lactic acid levels in human blood samples. This approach of forming heterojunctions by combining organic semiconductor materials with perovskites opens up new possibilities for constructing advanced perovskite-based photoelectric sensors.
Section
Graphical abstract
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Natural Products and Bioprospecting
·
2025
·Vol. 15
·期 1
·66
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IF 4.9
Xia Xuewei,Lin Jieling,Yang Lihong,Li Youhong,Lin Wenshen,Wang Qingqing,Liu Jun,Huang Riming
Syngnathoides biaculeatus , a potential functional food from marine sources, was found to enhance nonspecific immunity, but its functional ingredients have rarely been reported. Therefore, this study focused on the preparation and physicochemical properties of its water-soluble natural ingredients with their immunomodulatory activities and potential mechanisms. Firstly, we optimized the extraction method of glycoproteins from S. biaculeatus and prepared the crude glycoprotein SYB, from which the fraction glycoprotein SYB-1 was further purified. The carbohydrates of SYB-1 were 8.46% comprising mannose, glucose, and galactose, with an average molecular weight of 9.423 kDa. Amino acid analysis demonstrated that its major amino acids are glycine, glutamic acid, aspartic acid, and proline, with a total amino acid content of 88.81%. Furthermore, SYB-1 could significantly increase the cell viability of macrophages, and promote the release of NO, TNF- α , and IL-6. Metabolomics revealed that it was associated with arachidonic acid metabolism. The CYP450 enzyme family members and PTGS2 may be key targets for the regulatory role. These results suggested that the glycoprotein of S. biaculeatus may be an attractive functional food supplement from natural sources for immunocompromised populations. Graphical
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npj Clean Water
·
2025
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IF 11.4
Lv Shiyu,Yuan Tian,Zhang Xueyan,Yang Pu,Fu Kerong,Yao Xia,Fu Weilin,Zhang Qiuxian,Luo Yanli,Wang Feng
Herein, LaFeO 3 photocatalysts (HX-LFO and SX-LFO) were synthesized using hydrothermal and sol–gel methods, respectively, for removing β-E2 from dairy cattle breeding wastewater. A combination of flocculation pretreatment and photocatalytic Fenton oxidation was used to achieve efficient β-E2 degradation. Results revealed that HX-LFO had high defect concentrations and achieved better photocatalytic Fenton oxidation efficiency for β-E2 than SX-LFO. The H0.7-LFO/H 2 O 2 /visible (Vis) system completely degraded 2 mg/L of β-E2 within 2 h and achieved a total organic carbon (TOC) mineralization rate of 99.7%. The intermediate products, degradation pathways, and ecological toxicity of β-E2 degradation were analyzed. The H0.7-LFO/H 2 O 2 /Vis system exhibited stable degradation performance when humic acid and multiple anions coexisted in the reaction solution. The developed system increased the β-E2 degradation rate to 83.38%–100%. Characterizations and density functional theory calculations revealed that the La-defect formation energy in La/O dual-defect LaFeO 3 was lower than that in LaFeO 3 containing only La defects. Moreover, O defects facilitated the formation of La defects that lowered the energy barrier for free-radical desorption, making it easier for free radicals to form and diffuse into the reaction solution. La/O dual-defect LaFeO 3 exhibited lower binding energy for β-E2, accelerating charge transfer within itself and from itself to β-E2.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·775
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IF 12.6
Mu Yulei,Zhang Zhen,Zhou Huiqun,Ma Liang,Liu Bangheng,Hu Xu,Xu Chenjie,Wang Dong-An
Glioblastoma (GBM) remains among the most aggressive brain malignancies, necessitating the development of novel therapeutic strategies. In this study, we designed a multifunctional magnetic-targeting nanoassembly (MnBPDF) by chelating Mn²⁺ with a dopamine-modified albumin coat, encapsulating a magnetic core constructed with magnetic Fe₃O₄ and doxorubicin. MnBPDF effectively induces oxidative stress and triggers immunogenic cell death (ICD), thereby releasing damage-associated molecular patterns that prime antigen-presenting cells (APCs) and subsequently activate tumor-specific cytotoxic T lymphocyte (CTL) responses. Moreover, Mn²⁺ simultaneously enhances the activation of cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway, triggering robust type I interferon production and amplifying antitumor immune responses. By boosting antigen presentation by dendritic cells (DCs) in the GBM microenvironment, this system promotes CTL infiltration and inhibits tumor progression. The synergistic effects of magnetic targeting, ICD induction, and enhanced immune activation highlight MnBPDF as a highly effective therapeutic strategy for GBM. Graphical
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