Drug Design Development and Therapy
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2022
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IF 4.319
Ruifeng Wu,Zhiqiang Zhang,Baohua Wang,Ge Chen,Yaozhong Zhang,Haowen Deng,Zilong Tang,Junjie Mao,Lei Wang
Purpose Lung cancer is the leading cause of cancer mortality worldwide. Drug resistance is the major barrier for the treatment of non-small cell lung cancer (NSCLC). The aim of this research is to develop an aptamer-decorated hybrid nanoparticle for the co-delivery of docetaxel prodrug (DTXp) and cisplatin (DDP) and to treat lung cancer.Materials and Methods Aptamer-conjugated lipid–polymer ligands and redox-sensitive docetaxel prodrug were synthesized. DTXp and DDP were loaded into the lipid–polymer hybrid nanoparticles (LPHNs). The targeted efficiency of aptamer-decorated, DTXp and DDP co-encapsulated LPHNs (APT-DTXp/DDP-LPHNs) was determined by performing a cell uptake assay by flow cytometry-based analysis. In vivo biodistribution and anticancer efficiency of APT-DTXp/DDP-LPHNs were evaluated on NSCLC-bearing mice xenograft.Results APT-DTXp/DDP-LPHNs had a particle size of 213.5 ± 5.3 nm, with a zeta potential of 15.9 ± 1.9 mV. APT-DTXp/DDP-LPHNs exhibited a significantly enhanced cytotoxicity (drug concentration causing 50% inhibition was 0.71 ± 0.09 μg/mL), synergy antitumor effect (combination index was 0.62), and profound tumor inhibition ability (tumor inhibition ratio of 81.4%) compared with the non-aptamer-decorated LPHNs and single drug-loaded LPHNs.Conclusion Since the synergistic effect of the drugs was found in this system, it would have great potential to inhibit lung tumor cells and in vivo tumor growth.
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高影响因子
DALTON TRANSACTIONS
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2022
·Vol. 51
·期 46
·17642-17652
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IF 4.569
Yanyan Pei,Yanqu Wang,Chengyuan Jiao,Yuchang Wang,Zhuwu Jiang
Owing to the ubiquitous existence and low concentrations of detrimental nitrogen pollutants in micro-polluted water, simple adsorption-oriented approaches are becoming increasingly appealing for the effective removal of NO3− from wastewater. Triethanolamine (TDA) modified carbon-based layered double hydroxide (LDH) composites (TDA@LDH/CS) were synthesized by a supersaturated co-precipitation method for efficient NO3− adsorption. The characterization results showed that TDA@LDH/CS, formed by the stacking of irregular nanosheets and lamellar aggregates, has a mesoporous structure and a specific surface area of 67.15 m2 g−1. The Langmuir and pseudo-second-order kinetic models were well fitted with the adsorption of NO3− by TDA@LDH/CS, with the maximum adsorption capacity reaching 14.45 mg g−1, and the adsorption process was consistent with the spontaneous exothermic entropy increasement. Furthermore, the synergistic adsorption mechanism of NO3− by the TDA-modified materials was proposed using XPS analysis, which indicated that TDA modification greatly enriched the surface of TDA@LDH/CS with tertiary amine groups (R3N) and hydroxyl groups (–OH), providing more adsorption sites and active sites. After five cycles, the NO3− removal rate could still reach 64.2%, which exhibited its high potential to be utilized as an adsorbent for the removal of nitrogen pollutants from micro-polluted water.
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GREEN CHEMISTRY
·
2022
·Vol. 24
·期 22
·8812-8819
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IF 11.034
Qiyu Liu,Liang Zhou,Xinyi Xie,Di Fan,Xinping Ouyang,Wei Fan,Xueqing Qiu
Selective production of glucose from cellulose in a molten salt hydrate (MSH) shows high efficiency but the separation of glucose is still challenging. To address this issue, we proposed a stepwise method, where cellulose was first hydrolyzed into glucan oligomers and separated via anti-solvent precipitation, followed by being hydrolyzed into glucose under mild conditions. For potential industrial applications, using raw biomass as a substrate is more attractive due to its commercial advantages. However, directly hydrolyzing raw corn stover in an MSH resulted in a low glucan oligomer yield and sugar concentration, together with insufficient separation efficiency. This work demonstrated that the generation of acetic acid during hemicellulose hydrolysis is the critical factor affecting glucan oligomer selectivity. To address this issue, a pre-extraction method using auto-hydrolysis was proposed to remove hemicellulose before MSH hydrolysis. After pre-extraction, the obtained glucan oligomer yield could be enhanced from 59.9% to 79.5%, with the separation efficiency increased from 53.7% to 80.6% by methanol precipitation. Besides, increasing the raw corn stover concentration led to an increase in acetic acid and resulted in glucan oligomer degradation. However, after removing hemicellulose, the concentration of glucan oligomers could be significantly enhanced from less than 72.1 to 121.5 mg mL−1. Thus, pre-extraction of hemicellulose followed by MSH hydrolysis provides a potential application for directly producing glucan oligomers from raw biomass with improved yield and separation efficiency.
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Drug Design Development and Therapy
·
2022
·
IF 4.319
Qin Yin,Yi-jin Wu,Shu Pan,Dan-dan Wang,Meng-qing Tao,Wei-ya Pei,Jian Zuo
Background Studies have shown that α-mangostin (MG) could exert anti-rheumatic effects in vivo by restoring immunity homeostasis, and have indicated that activation of the choline anti-inflammatory pathway (CAP) may contribute to this immunomodulatory property. The current study was designed to further investigate the effects of MG on the CAP in peripheral immune cells and clarify its relevance to the potential anti-rheumatic actions.Methods The catalytic activity of acetylcholinesterase (AChE) and expression of α7-nicotinic cholinergic receptor (α7nAChR) in peripheral blood mononuclear cells (PBMCs) from rats with collagen-induced arthritis (CIA) or human volunteers were evaluated after MG treatment. Consequent influences on the immune environment were assessed by flow cytometry and ELISA analyses. Indirect effects on joints resulting from these immune changes were studied in a co-culture system comprised of fibroblast-like synoviocytes (FLSs) and PBMCs.Results MG promoted α7nAChR expression in PBMCs both in vivo and in vitro, and inhibited the enzymatic activity of AChE simultaneously. Activation of the CAP was accompanied by a significant decrease in Th17 cells (CD4+IL-17A+), while no obvious changes concerning the distribution of other T-cell subsets were noticed upon MG treatment. Meanwhile, MG decreased the secretion of TNF-α and IL-1β under inflammatory conditions. PBMCs from MG-treated CIA rats lost the potential to stimulate NF-κB activation and pro-inflammatory cytokine production of FLSs in the co-culture system.Conclusion Overall, the evidence suggested that MG can improve the peripheral immune milieu in CIA rats by suppressing Th17-cell differentiation through CAP activation, and achieve remission of inflammation mediated by FLSs.
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AQUATIC TOXICOLOGY
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2022
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IF 5.202
Chenyang Rao,Xianglin Cao,Lulu Li,Jiameng Zhou,Dandan Sun,Baohua Li,Suqi Guo,Rongjie Yuan,Han Cui,Jianjun Chen
As common environmental endocrine-disrupting chemicals (EDCs), bisphenol AF (BPAF) raises potential concerns for aquatic organisms due to its widespread presence and continued release in the aquatic environment. This research aimed to use zebrafish embryos and adult fish to explore the effects of environmentally relevant concentrations (5 μg/L), 50 μg/L and 500 μg/L of BPAF on zebrafish embryonic development, behavioral alterations, and the potential mechanisms driving these effects. The results showed that 500 μg/L of BPAF severely affected the growth and development of embryos. In behavioral experiments, all concentrations of BPAF significantly inhibited the locomotor activity of larvae, 50 and 500 μg/L BPAF significantly altered the anxiety-like and aggressive behavior of adult zebrafish. Furthermore, environmentally relevant concentrations and higher concentrations of BPAF induced varying degrees of oxidative stress in both embryonic and adult fish. The most significant histopathological changes and decreased acetylcholinesterase (AChE) activity were observed in the brain at 50 and 500 μg/L of BPAF. We hypothesized that oxidative stress is an important cause of behavioral disturbances in larvae and adult fish. To our best knowledge, the present experiment is a pioneer in studying the effects of BPAF on a variety of complex behaviors (swimming performance, anxiety-like, social behavior, aggression) in zebrafish, which emphasizes the potential health risk of higher concentrations of BPAF in terms of induced neurotoxicity.
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BIOMATERIALS
·
2022
·
IF 15.304
Lei Cao,Huixiang Tian,Man Fang,Zhe Xu,Dongsheng Tang,Juan Chen,Jiye Yin,Haihua Xiao,Kun Shang,Hongbin Han,Xiangping Li
cGAS-STING pathway, as an essential intracellular immune response pathway, has attracted much attention in tumor immunotherapy. However, low metabolic stability of conventional STING agonists limits their clinical application. Recent study shows that chemotherapeutic drugs cisplatin and camptothecin (CPT) can activate cGAS-STING pathway and induce immune response by DNA damage. Nevertheless, the ability of chemotherapeutic drugs to activate STING is so weak that new strategies are required to improve drug delivery efficiency for enhanced DNA damage, and then efficiently activate cGAS-STING pathway. Herein, we have developed a hybrid platinum prodrug (CPT-Pt (IV)) which can be triggered to release cisplatin and CPT in tumor cells. CPT-Pt (IV) with high hydrophobicity is further self-assembled with a ROS sensitive polymer (P1) and mPEG 2k -DSPE into ROS responsive nanoparticles (NPs). NPs could accumulate in the tumor site to release cisplatin and CPT, resulting in DNA double damage and finally activating cGAS-STING pathway, inducing DC cells maturation and increasing tumor infiltration of CD8 + T cells on colorectal cancer mouse model. This study showed that common DNA targeted drugs can activate the cGAS-STING pathway in situ via nano delivery system, and enhance the effect of chemotherapy and immunotherapy, which provide a new strategy for clinical antitumor therapy.
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Advanced Healthcare Materials
·
2022
·Vol. 12
·期 2
·2202117
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IF 11.092
Shanshan Peng,Lizhen Wang,Lin Liu,Liang Song,Junpeng Shi,Hanrun Zheng,Jixuan Xu,Rui Rong,Yun Zhang
Manipulation of autophagic processes has emerged as a promising strategy for synergizing nanoagent-mediated photothermal therapy (PTT). Most of the current studies focus on improving PTT efficacy by inhibiting pro-survival autophagy induced by the heat generated from the photothermal process. However, autophagy induced by the nanoagents is usually ignored, which may weaken the effect of autophagy-mediated efficacy improvement in PTT if induced autophagy is pro-death. Therefore, this work aims at developing a nanoagent that is able to induce heat-synergetic pro-survival autophagy to optimize the efficacy of PTT. An approach is developed to coat carbon layer, polyethylenimine (PEI), and folic acid (FA) on NaYF 4 :Er,Yb,Nd@NaNdF 4 (DCNPs@C@PEI@FA, DCPF) nanoparticles successively, giving access to the nanoagent to induce pro-survival autophagy. The synthetic imaging-guided photothermal nanoagent displays outstanding targeting ability and biocompatibility based on the surface modification of PEI and FA. By using an autophagy inhibitor chloroquine, a conspicuously synergistic effect on DCPF-mediated PTT in vitro and in vivo tumor models (HeLa) is achieved. A promising strategy is presented here to enhance the efficacy of imaging-guided PTT by modulating the autophagy induced by the nanoagent.
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JOURNAL OF CONTROLLED RELEASE
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2022
·
IF 11.467
Linlin Song,Dan Zheng,Jinshun Xu,Tianyue Xu,Zhihui Liu,Huan Zhang,Yi Li,Yulan Peng,Hubing Shi
Despite revolutionary achievements have been made in clinical cancer therapy, the immune checkpoint blockade regimen still presents limited efficacy on tumors lack of neoantigens exposure. Here, we designed and synthesized an on-demand microwave-controlled ozone release nanosystem to specifically generate reactive oxygen species in tumor mass. By taking advantage of iRGD modification, the synthesized nanosystem can be specifically enriched in the tumor microenvironment and subsequently internalized by tumor cells. Triggered by the low-power microwave, ozone was released from the nanocarriers and inhibited tumor cell growth in vitro and in vivo . Molecular mechanism investigation further unraveled that the released-ozone induced cytolytic cell death through the rapid generation of reactive oxygen species such as hydroxyl radical. The tumor-specific neoantigen derived from this immunogenic cell death promoted cytotoxic T-lymphocytes infiltration, which provided a fundament for immune checkpoint blockade therapy. In the triple-negative breast cancer animal model, tumor-specific delivery of ozone significantly improved the systematical anti-tumor efficacy of the PD-1 blockade antibody. Notably, tumor-locally confined microwave-controlled release avoided systematic toxicity in the tested animals. Collectively, our nanosystem provides a novel controllable strategy for promoting immune checkpoint blockade therapy, especially in tumor types deficient in infiltrated T-lymphocytes.
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高影响因子
Catalysts
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2022
·Vol. 12
·期 10
·1207
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IF 4.501
Chenliu Tang,Zhicheng Long,Yidan Wang,Dongze Ma,Xiaobiao Zhu
This study investigated the inhibition effects of sulfate on ferrous ion-activated persulfate oxidation of azo dye reactive brilliant red X-3B. The experimental results showed that the degradation efficiency of reactive brilliant red X-3B decreased from 100% to 63% in 60 min when the initial concentration of sulfate increased from 0 to 3 g/L. The ferrous/persulfate molar ratio had remarkable influence on persulfate oxidation capability in presence of sulfate. SO42−could coordinate with Fe2+and Fe3+in generating FeSO4ion pairs as well as FeSO4+or Fe(SO4)2−complexes, which were difficult to activate persulfate and reduced the regeneration of Fe2+. Radicals quenching and electron paramagnetic resonance experiments showed that ·OH and SO4·−were responsible for the oxidation of reactive brilliant red X-3B; however, the addition of sulfate significantly inhibited the generation of SO4·−, and then the generation of ·OH. Moreover, the corresponding Nernst equation indicated that high concentration of sulfate reduced the oxidation potential of SO4·−/SO42−. Experimental results proved that the adverse effects of sulfate on the persulfate oxidation could be counteracted either by batch addition of ferrous or by adding Ba2+to remove SO42−in the system.Keywords:persulfate;sulfate;sulfate radicals;reactive brilliant red X-3B
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INDUSTRIAL CROPS AND PRODUCTS
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2022
·
IF 6.449
Dongxue Cao,Enwei Wei,Zeyu Wang,Zhengyu Hu,Ling Qi,Hongli Zhou,Jingli Zhao
This paper aimed to elaborate the chemical characteristics of ginseng polysaccharide via chemical methods and instrumental analyses; additionally, the anti-glioma activity and immunostimulatory capacity were also evaluated via biological experiments. Ginseng polysaccharide by microwave-assisted extraction (MEGP) method was purified by spongiform diethylaminoethyl cellulose (DEAE) and further separated by DEAE-52 column chromatography to obtain four MEGP fractions (MEGP-0, MEGP-1, MEGP-2, MEGP-3). Utilizing Zeta potential analyzer and X-ray photoelectron spectroscopy (XPS), MEGPs were, for the first time, confirmed the valence state of C and O with the binding mode of elements, and all MEGP fractions contained triple-helix structure. MEGP-2 and MEGP-3 significantly reduced the cell growth rate of TJ905 and U87 , showing an inhibitory effect on glioma. Among four fractions, MEGP-2 markedly elevated the immunostimulatory capacity via the activation of MAPK signaling route, with an increasing the expression levels of p-p38, p-ERK and p-JNK. In summary, this paper laid a solid foundation for the development of ginseng, as a promising drug or tonic, in the fields of anti-glioma and immunoregulation.
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2022
·
IF 3.322
Jing Cong,Yuehui Zhang,Xinming Yang,Yu Wang,Hui He,Mengying Wang
Polycystic ovary syndrome (PCOS) is a complicated endocrinopathy affecting women at reproductive age. Increasing evidence has shown the anti-PCOS effect of electroacupuncture (EA), a modified approach of traditional Chinese medical therapy “acupuncture”. However, the underlying mechanism of EA-alleviated PCOS waits further explored. In this study, experimental PCOS were induced in rats by dehydroepiandrosterone (DHEA) injection. Testosterone (T)-induced human ovarian granulosa cell (GC) line KGN was used to mimic PCOS in vitro . EA significantly alleviated histological changes and hormone disruption in PCOS rats. Besides, EA inhibited cell apoptosis, autophagy and the activation of endoplasmic reticulum (ER) stress-related PERK/eIF2α/ATF4/CHOP signaling in ovaries of PCOS rats. More interestingly, intermedin (IMD), a member of calcitonin gene-related peptide (CGRP), was evidently up-regulated in ovarian GCs after EA treatment, and its main bioactive form IMD 1-53 suppressed cell apoptosis, autophagy and PERK/eIF2α/ATF4/CHOP signaling in T-induced KGN cells. Consistent with IMD 1-53 , ER stress inhibitor 4-PBA exerted an inhibitory effect on T-induced cell apoptosis and autophagy in KGN cells. Collectively, this study validates the protective effect of EA on DHEA-induced PCOS, and proposes that IMD relieved apoptosis and autophagy in T-induced granulosa cells via inhibiting ER stress.
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Journal of Environmental Chemical Engineering
·
2022
·
IF 7.968
Jie Tian,Junyan Wei,Yeping Liang,Ruixue Guo,Beibei Li,Ruijuan Qu,Dongmei Zhou,Zunyao Wang,Ping Sun
This study aimed to investigate the catalytic performance of Fe 3 O 4 /ZnO in the ozonation of 1-hexyl-3-methyl imidazolium bromide ([HMIM]Br). The Fe 3 O 4 /ZnO composite was prepared by a quick and convenient two-step coprecipitation method, and the optimum mass ratio of Fe 3 O 4 :ZnO was determined as 1:8. The structure, surface morphology, and chemical composition of the Fe 3 O 4 /ZnO composites were studied by characterization methods. The degradation efficiency of [HMIM]Br reached 96% in 5 min in catalytic ozonation with the Fe 3 O 4 /ZnO composite, an increase of 20% compared with ozonation alone. Moreover, the pseudo-first-order rate constant was 1.5 times higher than ozonation alone. With the increase in the catalyst dosage, the degradation efficiency of [HMIM]Br first improved and then decreased, and the optimum dosage was 0.25 g/L. The increase in pH value and the introduction of Cu 2+ effectively promoted the catalytic ozonation of [HMIM]Br with Fe 3 O 4 /ZnO, while the reducing inorganic ions inhibited the ozonation reaction. Different water matrices had little influence on the catalytic performance of Fe 3 O 4 /ZnO. Radical scavenging and EPR experiments showed that hydroxyl radicals (•OH), superoxide radicals (O 2 •- ) and singlet oxygen ( 1 O 2 ) were the main active species in this system. Meanwhile, product identification showed that the degradation of [HMIM]Br mainly included hydroxylation, imidazole ring cleavage and bond cleavage. Toxicity prediction illustrated that the catalytic ozonation of [HMIM]Br could effectively reduce its toxicity. The efficiency, toxicity, reusability, and stability experiments suggested that the developed catalyst, Fe 3 O 4 /ZnO composite, had promising prospects in catalytic ozonation.
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