JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Shan-Shan Yang,Qing-Yun Lv,Juan Fu,Tian-Yi Zhang,Yi-Si Du,Xin-Juan Yang,Le Zhou
As our further research, a series of new 7-chloro-9-methyl-2-phenyl-3,4-dihydro-β-carbolin-2-iums were designed and synthesized. Twelve compounds were found with excellent inhibition activity in vitro on three to five out of six phytopathogenic fungi, superior to standard drugs thiabendazole and/or azoxystrobin. Especially, 18 displayed the highest activity against three out of the fungi and the highest comprehensive activity for all of the fungi. The test in vivo revealed that 18 at 50 μg/mL was able to completely control Physalospora piricola infections in apples over 8 days. Scanning/transmission electron microscopic observations found that 18 could damage the hyphal integrity and cell membrane structure of P. piricola. The safety evaluation showed that 18 had no effect on the germination rate of cowpea seed at ≤200 μg/mL. The SAR revealed that the combination of 7-Cl and 2′- or 4′-alkyl is conducive to improvement of the activity. Thus, 7-chloro-9-methyl-2-phenyl-3,4-dihydro-β-carbolin-2-ium is a promising antifungal lead scaffold.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2018
·
IF 3.412
Qianqian Geng,Jingchan Xie,Xin Wang,Mengling Cai,Hui Ma,Hanwen Ni
A water-soluble inclusion complex for butachlor was prepared by complexation with (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD). Phase solubility results indicated a 1:1 stoichiometric ratio with an apparent stability constant of 864.3 M–1 in the obtained solid complex. The formation of the complex was confirmed by 1H nuclear magnetic resonance, Fourier transform infrared, and differential scanning calorimetry spectra. Coupled with the molecular docking results, butachlor was considered to be completely included in HP-β-CD cavity. Butachlor complexation with HP-β-CD decreased its adsorption capacity and enhanced its mobility in soil. The inclusion complex displayed better herbicidal activities than free butachlor. The 96 h median lethal concentration values of the inclusion complex and free butachlor was 2.30 and 0.65 mg L–1, respectively, for zebrafish, indicating that the complexation could significantly reduce toxicity to fishes. The present study provides an approach to develop environment-friendly formulations using CDs for herbicides.
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BIOORGANIC CHEMISTRY
·
2018
·
IF 3.929
Jianpeng Su,Jiayun Liu,Cheng Chen,Yuejuan Zhang,Kewu Yang
The superbug infection caused by New Delhi metallo-β-lactamase (NDM-1) has grown into an emerging threat, labelling and inhibition of NDM-1 has proven challenging due to its shuttling between pathogenic bacteria. Here, we report a potent covalent scaffold, ebsulfur, for targeting the protein in vitro and in vivo . Enzymatic kinetic study indicated that eighteen ebsulfurs gained except 1a – b and 1f inhibited NDM-1, exhibiting an IC 50 value ranging of 0.16–9 μM, and 1g was found to be the best, dose- and time-dependent inhibitor with an IC 50 of 0.16 μM. Also, these ebsulfurs effectively restored the antibacterial activity of cefazolin against E. coli expressing NDM-1, and the best effect was observed to be from 1g , 1i and 1n , resulting in an 256-fold reduction in MIC of the antibiotic at a dose of 16 μg/mL. The equilibrium dialysis study implied that the ebsulfur disrupted the coordination of one Zn(II) ion at active site of NDM-1. Labelling of NDM-1 using a constructed fluorescent ebsulfur Ebs - R suggested that the inhibitor covalently bound to the target through SDS-PAGE analysis in vitro . Also, labelling NDM-1 in living E. coli cells with Ebs - R by confocal microscopic imaging showed the real-time distribution change process of intracellular recombinant protein NDM-1. Moreover, the cytotoxicity of these ebsulfurs against L929 mouse fibroblastic cells was tested, and their capability to restore antibacterial activity of antibiotic against clinical strains E. coli EC08 producing NDM-1 was determined. The ebsulfur scaffold proposed here is valuable for development of the covalent irreversible inhibitors of NDM-1, and also for labelling the target in vitro and in vivo .
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GREEN CHEMISTRY
·
2019
·Vol. 21
·期 19
·5336-5344
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IF 9.405
Da Yang,Huan Liu,Lei Liu,Wen-Di Guo,Yong Lu,Ye Liu
An amphiphilic tri-functional ligand (L1) containing a Lewis acidic phosphonium cation, a phosphino-fragment and a hydrophilic sulfonate anion (–SO3−) enabled Pd(OAc)2 to efficiently co-catalyze the hydroxycarbonylation of terminal alkynes towards α,β-unsaturated carboxylic acids. These incorporated functional groups synergistically promoted the reaction, which proved more effective than the ligands lacking –SO3− and/or phosphonium and the mechanical mixtures of the individual functional groups independently. The molecular structure of Pd-L1 indicated that –SO3− in L1 served as a secondary O-donor ligand with reversible coordinating ability, cooperating with the phosphino-fragment to stabilize the Pd-catalyst. The in situ FT-IR analysis verified that the formation and stability of Pd–H active species in charge of hydroxycarbonylation were dramatically facilitated by the presence of L1. It was believed that, over the L1-based Pd-catalyst, H2O was cooperatively activated by the Lewis acidic phosphonium via “acid–base pair” interaction (H2O → P(V)+) and by the hydrophilic SO3−via hydrogen bonding (SO3−⋯H2O), giving rise to the formation of dimeric and mono-nuclear Pd–H species driven by reversible SO3−-coordination. In addition, the L1-based Pd-catalyst could be immobilized in the ionic liquid [Bmim]NTf2 for six-run recycling uses without obvious activity loss and detectable metal leaching.
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Catalysis Science & Technology
·
2022
·Vol. 12
·期 9
·2903-2911
·
IF 6.177
Zhenzhen Yang,Yuhan He,Pengfei Tang,Bangchong Zhu,Genlei Zhang,Jianbo He
Direct conversion of biomass-based fructose to 2,5-diformylfuran (DFF) has attracted much attention in recent years. In this study, molybdenum carbide anchored on glucose-derived carbon (β-Mo2C@C) was synthesized by a simple, green and economical method and was found to be highly active for production of DFF from 5-hydromethylfurfural (HMF) and fructose. DFF was obtained with high yields of 94.5% and 76.0% from aerobic oxidation of HMF and “one-pot” conversion of fructose under optimal reaction conditions, respectively. A probable mechanism involving Mo6+/Mo2+ redox cycles was proposed for β-Mo2C@C-catalyzed oxidation of HMF to DFF according to the experimental and characterization results. Furthermore, the β-Mo2C@C catalyst can be reused without much loss of catalytic activity, suggesting that β-Mo2C@C is highly stable.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Jialin Hu,Lingling Guo,Xinxin Xu,Hua Kuang,Liguang Xu,Liqiang Liu,Chuanlai Xu,Hongyu Zhang,Maozhong Sun
Food fraud practices, such as milk adulteration, exacerbate allergy risks by introducing undeclared allergens, primarily β-lactoglobulin (β-Lg). Therefore, the effective detection of β-Lg in food is crucial for ensuring consumer safety. In this study, we screened hybridoma cell lines 11F10 and 4G1, which recognized β-Lg variants A (β-Lg A) and B (β-Lg B). Genes of these hybridoma cell lines were extracted, amplified, and cloned into expression vectors to produce recombinant antibodies (rAbs). Using rAbs 11F10 and 4G1, colloidal gold-based immunochromatographic strips were developed for detecting β-Lg A and B in dairy products. The strips exhibited a visual limit of detection of 200 μg/kg in cheese and amino acid formulas, and 200 ng/mL in raw milk. The results of molecular docking indicated that the critical amino acid residues for 11F10 were Asp57, Tyr32, and Arg49, whereas those for 4G1 included Tyr101, Tyr106, and Tyr49.
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INTERNATIONAL DAIRY JOURNAL
·
2025
·
IF 3.4
Qianqian Ding,Jiuxiu Shi,Mengjiao Lv,Ling Li,Danli Wang,Tinglan Yuan,Gongshuai Song,Jinyan Gong
Protein-based complexes are critical for enhancing food functionality, particularly owing to the synergistic effects of ternary covalent complexes containing proteins, polysaccharides, and polyphenols. This study investigated the formation mechanism, structural characteristics, and functional properties of ternary covalent complexes composed of β-lactoglobulin (β-Lg), hyaluronic acid (HA), and three dicaffeoylquinic acid (DCQA) isomers (3,5-DCQA, 3,4-DCQA, and 4,5-DCQA); the complexes were prepared using different addition sequences. These complexes exhibited reduced surface hydrophobicity, altered secondary structures, and enhanced thermal stability. They showed significantly better antioxidant activities and emulsifying properties in comparison with native β-Lg. The protein-polysaccharide-polyphenol sequence yielded more stable and compact structures with higher polyphenol binding capacity. Subsequently, the protein-polysaccharide-polyphenol complexes were used to encapsulate curcumin (Cur), and it yielded encapsulation efficiencies up to 76.14 % (β-Lg-HA-3,4-DCQA-Cur), which was significantly higher than the encapsulation efficiency of native β-Lg-Cur (36.35 %). These complexes also improved the physical stability and antioxidant activity of Cur under adverse conditions. These findings highlight the critical roles of the addition sequence and polyphenol structure in modulating the functionality of protein-based complexes, and offer promising strategies for enhancing the delivery of bioactive compounds in food applications.
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International Journal of Stem Cells
·
2021
·
IF 2.5
Qiong Li,Lei Huang
Background and Objectives MiR-148a-3p has been reported to regulate the differentiation of marrow stromal cell osteoblast. In this study, whether miR-148a-3p regulated the odontoblastic differentiation of human dental pulp stem cells (hDPSCs) or not was explored. Methods and Results The hDPSCs were isolated and identified via flow cytometry. Targets of miR-148a-3p were identified via bioinformatics and dual-luciferase reporter assay. After the cell was cultured in the odontogenic differentiation medium or infected, cell viability, invasion, and odontoblastic differentiation were detected via MTT, transwell, and Alizarin Red S staining, respectively. The miR-148a-3p, Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions were determined by RT-qPCR and Western blot. The hDPSCs odontoblastic differentiation downregulated the miR-148a-3p expression and upregulated Wnt1 expression. Wnt1 was determined as the target for miR-148a-3p. MiR-148a-3p mimic and siWnt1 suppressed the cell viability, invasion, and odontoblastic differentiation of hDPSCs and inhibited the Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions. In contrast, miR-148a-3p inhibitor and overexpressed Wnt1 promoted the cell viability, invasion, and odontoblastic differentiation of hDPSCs, and upregulated the Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions. Also, miR-148a-3p mimic and inhibitor reversed the effects of Wnt1 overexpression and siWnt1. Conclusions MiR-148a-3p modulated the invasion and odontoblastic differentiation of hDPSCs through the Wnt1/β-catenin pathway.
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TALANTA
·
2018
·
IF 4.244
Miao Wang,Kun Su,Jing Cao,Yongxin She,A.M. Abd El-Aty,Ahmet Hacımüftüoğlu,Jing Wang,Mengmeng Yan,Sihui Hong,Shuibing Lao,Yanli Wang
Here, we developed a novel non-enzymatic rapid testing method for determination of organophosphate pesticide (malathion) in water. In principle, target molecule can block the Fluorescence resonance energy transfer (FRET) between chemical fluorescent probe (energy donor) and β-cyclodextrin-coated silver nanoparticles (@AgNP) (receptor). The effects of malathion on the dynamics of fluorescent probe and β-cyclodextrin@AgNP were evaluated and their properties were further characterized. The current methodology showed a good sensitivity of 0.01 μg/mL represented as a limit of detection (LOD) and the calibration curve was linear over the concentration range of 0.1–25 μg/mL. Recovery rate from water samples spiked at 3 different concentration levels (0.3, 0.4, and 0.6 μg/mL) showed satisfactory range between 83% and 101%.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2016
·Vol. 27
·期 8
·8254-8270
·
IF 1.798
Xi Junhua,Ding Mingye,Dai Jianbin,Pan Yajing,Chen Daqin,Ji Zhenguo
In this paper, Yb 3+ /Er 3+ co-doped hexagonal NaYF 4 have been successfully synthesized by solvothermal, thermal decomposition, hydrothermal and molten salt method, respectively. The crystal structures, particle morphologies, sizes and corresponding upconversion luminescent properties of β-NaYF 4 :Yb 3+ /Er 3+ nano/microcrystals are systemically studied. It is interesting to observed that the temperature-dependent UC luminescent properties of β-NaYF 4 :20 %Yb 3+ , 2 %Er 3+ samples are strongly related to their synthesis methods. Comparison of the upconversion luminescence and thermal sensitivity between the β-NaYF 4 :Yb 3+ /Er 3+ samples reveals that β-NaYF 4 :Yb 3+ /Er 3+ sub-microplates synthesized by thermal decomposition method have much stronger green emission intensity, better luminescent thermal stability and higher temperature sensitivity. This findings are relevant to the application of β-NaYF 4 :Yb 3+ /Er 3+ in optical temperature sensors and to the further understanding of the UC luminescent mechanism.
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GENE
·
2024
·
IF 2.6
Wen Zhong,Huanan Jia,Haiyan Zhu,Yuan Tian,Wei Huang,Qiyue Yang
Sarcopenia has become a prominent health problem among the elderly because of its adverse consequence, including physical disabilities and death. Fibro-adipogenic progenitors (FAPs) exhibit adipogenic and fibrogenic potencies and regulate skeletal muscle development, which plays important role in sarcopenia. Mairin, as an ingredient of Astragalus membranaceus, has the effect of anti-fibrosis. Therefore, we predicted that mairin targeted the fibrosis of FAPs and then affected sarcopenia. To verify our ideas, mairin (30 mg/kg/day or 60 mg/kg/day) was given to senescence accelerated mouse-prone 8 (SAMP8) mice by oral administration. Aging led to loss of weight, skeletal muscle mass, strength, and function, and an increase in muscle atrophy and fibrosis, while mairin administration inhibited physiological decline caused by aging. Similarly, mairin (20 μM or 40 μM) treatment enhanced FAP proliferation but blocked the differentiation into fibroblasts. Mechanically, mairin played an anti-fibrotic role via AMP-activated protein kinase-transforming growth factor beta-drosophila mothers against decapentaplegic protein (AMPK-TGF-β-SMAD) axis, as evidenced by increased phosphorylation of AMPKα and decreased TGF-β and phosphorylated-SMAD2/3. In addition, the potential target genes of mairin were explored by mRNA sequencing in our study. In conclusion, mairin may interfere with the AMPK/TGF-β/SMAD pathway to repress the fibrosis of FAPs and eventually ameliorate sarcopenia.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Bin Yang,Jia Liu,Xin Liu,Shiyue Niu,Liqian Niu,Xian Wang,Xinxin Liu,Shuyun Bi
Acetamiprid and pymetrozine are effective pyridine insecticides used to kill aphids and whiteflies in agricultural products such as fruits, vegetables and rice, whose residues pose a serious threat to human health. Herein, the silver nanoparticles (AgNPs) modified by β -cyclodextrin ( β -CD) and nano-CuO (named as β -CD/CuO@AgNPs) were synthesized and it was used as surface-enhanced Raman spectroscopy (SERS) substrate to determine acetamiprid and pymetrozine. Among four SERS substrates such as AgNPs, β -CD/AgNPs, CuO@AgNPs and β -CD/CuO@AgNPs, β -CD/CuO@AgNPs exhibited the most substantial enhancement of the Raman signals for acetamiprid and pymetrozine, with the enhancement factors (EFs) of 2.16 × 10 6 and 3.86 × 10 6 , respectively. The XRD, TGA, TEM, HAADF-STEM, UV–vis and FT-IR were used to characterize β -CD/CuO@AgNPs and the combination of the substrate with acetamiprid/pymetrozine. Single factor experiment and response surface method were used to optimize pH, dosage of coagulant and nanosubstrate and mixing time. The influences of various interfering substances on the detection system were studied. The excellent linear relationships were respectively established for acetamiprid and pymetrozine in the range of 6.67 × 10 −9 –6.67 × 10 −7 and 1.00 × 10 −8 –1.00 × 10 −6 mol L −1 , with the limits of detection (LODs) were 0.36 and 0.94 nmol L −1 (S/N = 3). This project was successfully used for the detection of acetamiprid and pymetrozine in apple, pear and peach samples. The recoveries in the fruit samples were in the range of 95.5 %–105.0 %.
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危险品化学品经营许可证（带存储）