PEST MANAGEMENT SCIENCE
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2022
·Vol. 79
·期 2
·666-677
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IF 4.462
Meng-Ling Chen,Shu-Xia Zhang,Peng-Yu Guo,Qing-Shi Qin,Li-Wei Meng,Guo-Rui Yuan,Jin-Jun Wang
BACKGROUND The oriental fruit fly, Bactrocera dorsalis (Hendel) is a worldwide pest damaging a wide range of hosts. Due to the long-term indiscriminate use of insecticides, B. dorsalis has developed serious resistance to several insecticides. UDP-glycosyltransferases (UGTs) are secondary metabolic enzymes involved in biotransformation and play an important role in the metabolism of plant secondary metabolites and synthetic insecticides in insects. Thus, we suspect that UGTs in B. dorsalis play an important role in insecticide tolerance. RESULTS In this study, 31 UGT genes were identified in the genome of B. dorsalis , belonging to 13 subfamilies. Real-time quantitative polymerase chain reaction (RT-qPCR) results revealed that 12 UGT genes were highly expressed in the antennae, midgut, Malpighian tubule and fat body. The mRNA expressions of 17 UGT genes were up-regulated upon exposure to λ-cyhalothrin, imidacloprid, abamectin and chlorpyrifos. Knockdown of the selected five UGT genes ( BdUGT301D2 , BdUGT35F2 , BdUGT36K2 , BdUGT49D2 , BdUGT50B5 ) by RNA interference increased the mortality of B. dorsalis from 9.29% to 27.22% upon exposure to four insecticides. CONCLUSION The abundance of UGTs in B. dorsalis is similar to other insect species, and 12 out of 31 UGTs were specifically expressed in metabolic tissues, suggesting a key role in detoxification. Down-regulation of five selected UGT genes increased the susceptibility of B. dorsalis to various insecticides, indicating that UGTs may play an important role in tolerance of B. dorsalis to multiple insecticides. © 2022 Society of Chemical Industry.
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CHEMOSPHERE
·
2022
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IF 8.943
Zixiao Ren,Haiyan Cao,Peter Desmond,Bingsheng Liu,Huu Hao Ngo,Xu He,Guibai Li,Jun Ma,An Ding
In this study, we investigated the effect of different compositions of aquatic natural organic matter (NOM) and ions on virus removal by ultrafiltration (UF). MS2 bacteriophage was used as a surrogate. Humic acid (HA) improved the MS2 removal rate from 1.95 ± 0.09 LRV to 2.40 ± 0.03 LRV at the HA dosage of 9 mg/L through the combined mechanisms of size exclusion, electrostatic repulsion and hydrophobicity . MS2 removal rate further increased to 3.10 ± 0.05 LRV by 10 mmol/L Na + dosage and 3.19 ± 0.12 LRV by Ca 2+ 1 mmol/L in the HA-containing UF system. Size exclusion turned into the dominant virus removal mechanism according to the results of the fouling model fitting and the weakening of electrostatic repulsion and hydrophobicity. The complexation of Ca 2+ also played a role in MS2 removal based on the analysis of interaction force. MS2 removal rate by bovine serum albumin (BSA) was poor, which was 2.07 ± 0.06 LRV at the BSA dosage of 9 mg/L. Hydrophobicity was greatly reduced and the dominant virus removal mechanisms were size exclusion and electrostatic repulsion. 10 mmol/L Na + in the presence of BSA deteriorated MS2 removal rate to 2.02 ± 0.07 LRV by the weakening of electrostatic repulsion, hydrophobicity and size exclusion. Electrostatic repulsion severely decreased by 1 mmol/L Ca 2+ and the enhanced adsorption barrier represented competitive adsorption of Ca 2+ by BSA and MS2 contributed for MS2 removal further decline (1.99 ± 0.05 LRV). Complex components in water will have different effects on virus removal due to their properties and interactions. This study can provide references for selecting more efficient water treatment methods according to the different compositions of raw water in actual water treatment applications during the UF process. Moreover, the retention of virus by UF can be predicted based on our study results.
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OPTICAL MATERIALS
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2022
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IF 3.754
Song Xie,Jing Shi,Yicun Liang,Xiaozhan Yang,Wenlin Feng
Terbium metal organic frameworks (Tb-MOFs) hollow spheres were synthesized by solvothermal method, using 5-aminoisophthalic acid (H 2 AIP) as organic ligand, 2-methylimidazole (2-MI) as competitive ligand and protective agent. Tb-MOFs exhibit excellent luminescent sensing properties to water in organic solvents , and the limit of detections (LODs) in N , N -dimethylformamide and ethanol are 0.000515% and 0.000422%, respectively. Water can not only enhance the luminescent properties of Tb-MOFs, and also a new emission peak at 418 nm (or 427 nm) appears. The enhancement and new peak are quenched by the Fe 3+ in water, and the LODs of Tb-MOFs for Fe 3+ at 402 nm and 545 nm are 0.45 μM and 0.71 μM, respectively. The sensing mechanism for water is water can replace part of the coordination of H 2 AIP with Tb 3+ , and the sensing mechanism for Fe 3+ is that the competitive absorption between Fe 3+ and H 2 AIP inhibits the energy absorption of the organic ligand.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2019
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IF 2.931
Aiqing Feng,Yongmei Jia,Liping Huang,Lin Wang,Guohua Zhou,Sheng Wang,Peilian Liu
Near-Infrared “turn on” type fluorescent probes are attractive and promising in the fields of chemical sensing and bioimaging. Here, a new dicyanomethylene-4H-pyran derivative (DCM-Si) NIR fluorescent probe was designed and synthesized for specific lighting up F − in living cells and bodies. Si O bond was used as F − trigger, and the release of fluorophore (DCM-NH 2 ) occurred after substituent reaction and subsequent 1,6-elimination. This NIR probe displayed high sensitivity and selectivity for the sensing of F − , and the detection limit was calculated to be as low as 157 nM. Moreover, the “off-on” fluorescent signal changes can be realized by adding F − in living cells and zebrafish embryos.
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BIORESOURCE TECHNOLOGY
·
2026
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IF 8.2
Yuhao Jia,Shuwen Luo,Xu Wang,Weihao Li,Bei Li,Dayi Zhang
In situ microcapsule-entrapped cultivation screened antibiotic-resistant bacteria. • 20 extra genera revealed higher resistant-bacterial diversity in activated sludge. • Active tetracycline resistance gene diversity was overestimated in activated sludge. • A novel tetracycline resistance gene tetG_N1 involved in tetracycline efflux. Antibiotic resistance poses a growing threat to global health and increases healthcare costs. Considering the limitations of traditional methods identifying antibiotic-resistant bacteria and genes, this study established an in situ microcapsule-entrapped cultivation (IMEC) strategy to screen the active tetracycline-resistant bacteria and genes in activated sludge from a wastewater treatment plant. Besides six tetracycline-resistant bacterial genera identified by conventional isolation and direct sequencing, IMEC uncovered extra 20 bacterial genera resistant to tetracycline, and their metabolic activities and tetracycline resistance were confirmed by Raman-Deuterium isotope probing. Among them, Romboutsia , Janthinobacterium , and Hydrogenoanaerobacterium were reported to have tetracycline resistance for the first time. The diversity of active tetracycline-resistant genes identified by IMEC was much lower than that by direct sequencing. In particular, one new tetG -like gene encoding a tetracycline outer periplasmic pump was confirmed by molecular docking. These findings documented the underestimated diversity of tetracycline-resistant bacteria but overestimated diversity of tetracycline-resistant genes in sludge. Overall, IMEC provides an effective tool for resolving active antibiotic-resistant bacteria and genes in activated sludge, offering new insights for water epidemiology. Download: Download high-res image (134KB) Download: Download full-size image
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npj Science of Food
·
2026
·
IF 9.7
Zhou Yunxiao,Lu Yiguang,Xiang Qing,Song Xiaochao,Yang Wei,Zhao Yangfei,Liang Chen,Bai Jian,Cheng Mengyu,Zhang Jianhai
As a widely distributed hazardous substance in agriculture and food, arsenic has become a global public health concern. Riboflavin is known for its anti-inflammatory and antioxidant properties, but whether it mitigates arsenic-induced male reproductive damage remains unclear. To clarify arsenic’s reproductive toxicity, network toxicology was first used to identify potential targets. Subsequently, thirty-six 4-week-old male mice were administered arsenic trioxide (5 mg/L in drinking water) and/or riboflavin (10 mg/kg in diet) for 22 weeks to establish a riboflavin intervention model. Network toxicology identified that arsenic induces male infertility via multiple signaling pathways, such as the TGF-β signaling pathway. In vivo, arsenic diminished sperm quality, caused histopathological damage, and impaired both blood-testis barrier integrity and Sertoli cell proliferation, while riboflavin effectively mitigated these detrimental effects. In vitro, TM4 cell experiments further confirmed that riboflavin reversed arsenic-induced decline in cell viability and cytoskeletal damage. This study is the first to demonstrate that the protective effect of riboflavin is mainly attributed to its ability to mediate the binding of Bmp4 to Bmpr1α, thereby reversing arsenic-induced toxicity, providing a new perspective for exploring riboflavin’s role in alleviating arsenic toxicity.
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SCIENCE OF THE TOTAL ENVIRONMENT
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2023
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IF 9.8
Yi Huang,Junxu Wu,Yuetong Lu,Runchao Wang,Yaqiong Lan,Ning Jia
Behavioral changes in zebrafish are an effective early warning system to determine water quality. However, only a few studies have examined the response of zebrafish to non-chemical stimulus after exposure to a contaminant. Therefore, this study investigated the differences in the behavioral responses of zebrafish to acoustic stimuli before and after exposure to cadmium (Cd). Acoustic escape response sensitivity curves were obtained and analyzed, followed by the determination of sensitive stimulus conditions at 100 Hz and 97 dB with a duration of 30 s and an interval of 30 min. Zebrafish exhibit a significant acoustic escape response, which is significantly reduced after exposure to Cd. The results showed that zebrafish stop demonstrating acoustic escape responses when exposed to higher Cd concentrations or longer acoustic exposures. Based on these results, a novel method for detecting abnormal behavior in zebrafish by acoustic stimulation has been proposed, which is expected to reduce the false alarm rate of this type of water quality technology .
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EUROPEAN POLYMER JOURNAL
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2023
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IF 6
Jiayi Wang,Yicen Ge,Yue Wang,Rui Sun,Xiao Yang,Haodan Xue,Xiaoyan Ma,Jinyu Liu,Keling Hu
Pd nanoparticles (NPs) supported by micelles formed with poly(2-(dimethylamino)ethyl methacrylate)- b -poly(lauryl methacrylate) (Pd/PDMAEMA- b -PLMA) were prepared and employed as catalyst in the Suzuki-Miyaura cross-coupling reaction of aryl bromides with arylboronic acids in water. Most reactions proceeded with high efficiency at room temperature with only 0.05 mol% Pd loading in the absence of ligands, which afforded the desired functionalized biaryls in 73–98 % yields after 24 h. After reaction, Pd/PDMAEMA- b -PLMA retained in the aqueous phase was easily separated from the insoluble biaryl product and could be further reused two times without significant loss in catalytic efficiency. Characterization of the catalyst disclosed that sub-2 nm Pd NPs were embedded in the hydrophilic corona of polymeric micelles that served as nanoreactors in water.
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JOURNAL OF HAZARDOUS MATERIALS
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2023
·
IF 13.6
Liangchen Long,Shutao Wang,Zhelu Gao,Shijie You,Liangliang Wei
A comprehensive study was conducted to investigate how ultraviolet (UV) irradiation combined with electrochemistry (EC) can efficiently remove human body fluids (HBFs) related pollutants, such as urea/creatinine/hippuric acid, from swimming pool water (SPW). In comparison with the chlorination, UV, EC, and UV/chlorine treatments, the EC/UV treatment exhibited the highest removal rates for these typical pollutants (TPs) from HBFs in synthetic SPW. Specifically, increasing the operating current of the EC/UV process from 20 to 60 mA, as well as NaCl content from 0.5 to 3.0 g/L, improved urea and creatinine degradation while having no influence on hippuric acid . In contrast, EC/UV process was resilient to changes in water parameters (pH, HCO 3 - , and actual water matrix). Urea removal was primarily attributable to reactive chlorine species (RCS), whereas creatinine and hippuric acid removal were primarily related to hydroxyl radical , UV photolysis, and RCS. In addition, the EC/UV procedure can lessen the propensity for creatinine and hippuric acid to generate disinfection by-products. We can therefore draw the conclusion that the EC/UV process is a green and efficient in-situ technology for removing HBFs related TPs from SPW with the benefits of needless chlorine-based chemical additive, easy operation, continuous disinfection efficiency, and fewer byproducts production.
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JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Xiaoying Zhang,Fangfei Cai,Funing Ma,Paul Reimus,Linlin Qi,Di Lu,Mohamad Reza Soltanian,Zhenxue Dai
Colloids play a crucial role in influencing the mobility of radionuclides in high-level radioactive waste repositories. However, the co-transport behavior of radionuclides and colloids in geological media remains insufficiently understood. This study investigated the transport of Strontium (Sr) in four types of granite minerals (quartz, biotite , K-feldspar, and plagioclase) in the presence and absence of Na-bentonite colloids (Na-BC) using column experiments. Employing a stepwise modeling strategy, this study first determined the basic flow and transport parameters through breakthrough curve analysis of the conservative tracer (Br). Then, the experimental data of Sr in the colloid-free Sr transport experiments and Na-BC in the Sr-colloid co-transport experiments were quantitatively explained using a two-site sorption model and a two kinetic sites model, respectively. Finally, the co-transport behavior of Sr and Na-BC was fitted using the colloid-facilitated solute transport model. The stepwise modeling allowed quantification of the kinetics of Sr sorption onto mobile and immobile Na-BC and highlighted the role of straining in Na-BC retention. In the absence of Na-BC, Sr transport experienced the greatest retardation in biotite, followed by plagioclase, K-feldspar, and quartz, respectively, which positively correlated with the specific surface area of the minerals. Moreover, Na-BC enhanced Sr transport with the same retardation order in all tested minerals, with recovery rate increments of 68.61%, 19.21%, 6.67%, and 4.33%, respectively. The transport of Sr in K-feldspar was found to be less affected by Na-BC compared to the other tested minerals, likely due to the strong cation exchange capacity of K + among the cation components of minerals, making hydrated K + more likely to exchange with Sr 2+ on K-feldspar surfaces. These findings hold significant implications for assessing the risks associated with the transport of radionuclides in deep geological repositories.
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Surfaces and Interfaces
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2023
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IF 6.2
Jiayao Zhang,Danni Xue,Huan Liu,Zhao Wei,Chenjie Gu,Shuwen Zeng,Junhui Jiang,Tao Jiang,Xingfei Zhou,Kerong Wu
Attributed to their structure-dependent chemical enhancement capability and perfect biocompatibility, semiconductors have attracted significant concern in surface enhanced Raman scattering (SERS)-based immunoassay. By continually adjusting the size of red phosphorus (RP) in the transition process from bulk to nanosheet, we investigated in detail about the improvement of high-efficient charge transfer (CT) resonance dependent on the perfect energy level matching within the substrate-molecule combination. The nano-sized RP has achieved an impressive three-fold enhancement in SERS intensity in comparison to the RP nanosheet. Furthermore, the RP-based immunosubstrate was combined with MoS 2 nanoflowers-based immunoprobe to construct a sandwich immunostructure for the accurate and efficient diagnostics of cancer marker prostate-specific antigen. A limit of detection as low as 3.99 × 10 −10 M was obtained in the SERS-based immunoassay with reliable specificity. This study presents an innovative approach to promote the SERS performance of semiconductors, which may find potential biological applications in the early screening of cancer.
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Food & Function
·
2023
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IF 6.1
Jiaxin Shen,Yanping Wu,Teng Wei,Yangzheng He,Xiaoru Liu,Zeyuan Deng,Jing Li
Phospholipids play a crucial role in the growth and neurodevelopment of infants. Currently, soybean phospholipids (SPLs) are the common phospholipid component in most infant formulas (IFs), which, however, shows an obvious difference with the phospholipid (PL) composition of human milk fat. Therefore, in the present study, human milk phospholipid analogs (HMPAs) were prepared by mimicking the composition of PE, PC, PI, PS, and SM in breast milk phospholipids and the composition of the major fatty acids (C16:0, C18:0, C18:1, and C18:2), and their digestion and absorption characteristics were explored using in vitro and mice models. The prepared HMPA contained 26.48% PE, 24.64% PC, 36.19% SM, 6.35% PI, and 6.32% PS, with 40.51% C16:0, 17.02% C18:0, 29.19% C18:1, and 13.26% C18:2, showing different digestive properties relative to SPL. There was little effect on the physical and chemical properties of HMPA under in vitro gastric conditions. The hydrolysis degree, fatty acids release rate, and average particle size decreasing rate of HMPA was significantly higher than that of SPL during digestion in vitro intestine (P < 0.05), showing better digestive process relative to SPL. In terms of the mice model, HMPA had a higher hydrolysis degree in the intestinal tract. Based on the area under curve (AUC) analysis of serum fatty acids, it was found that despite HMPA being absorbed at a slower rate than SPL, it was absorbed more than SPL. In summary, the digestion and absorption of HMPA were preferred to SPL, and these obtained results might provide a theoretical basis for the development and utilization of HMPA in IF.
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