Nature Communications
·
2023
·Vol. 14
·期 1
·1-16
·
IF 16.6
Chen Shengqiang,Yu Yuanman,Xie Songqing,Liang Danna,Shi Wei,Chen Sizhen,Li Guanglin,Tang Wei,Liu Changsheng,He Qianjun
The senescence microenvironment, which causes persistent inflammation and loss of intrinsic regenerative abilities, is a main obstacle to effective tissue repair in elderly individuals. In this work, we find that local H 2 supply can remodel the senescence microenvironment by anti-inflammation and anti-senescence effects in various senescent cells from skeletally mature bone. We construct a H 2 -releasing scaffold which can release high-dosage H 2 (911 mL/g, up to 1 week) by electrospraying polyhydroxyalkanoate-encapsulated CaSi 2 nanoparticles onto mesoporous bioactive glass. We demonstrate efficient remodeling of the microenvironment and enhanced repair of critical-size bone defects in an aged mouse model. Mechanistically, we reveal that local H 2 release alters the microenvironment from pro-inflammation to anti-inflammation by senescent macrophages repolarization and secretome change. We also show that H 2 alleviates the progression of aging/injury-superposed senescence, facilitates the recruitment of endogenous cells and the preservation of their regeneration capability, thereby creating a pro-regenerative microenvironment able to support bone defect regeneration.
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Scientific Reports
·
2023
·Vol. 13
·期 1
·1-11
·
IF 4.6
Lan Xiang,Qi Dongdong,Ren Hao,Liu Tao,Shao Hong,Zhang Jiantao
Damage to lamellar keratinocytes, an essential cellular component of the epidermal layer of hoof tissue, can have a detrimental effect on hoof health and the overall production value of dairy cows. We isolated and cultured cow lamellar keratinocytes using the Dispase II and collagenase methods. We purified them by differential digestion and differential velocity adherent methods at each passaging and identified them by keratin 14 immunofluorescence. We established an in vitro model of inflammation in laminar keratinocytes using LPS and investigated whether chicoric acid protects against inflammatory responses by inhibiting the activation of the TLR4/MAPK/NF-κB signaling pathway. The results showed that cow lamellar keratinocytes were successfully isolated and cultured by Dispase II combined with the collagenase method. In the in vitro inflammation model established by LPS, the Chicoric acid decreased the concentration of inflammatory mediators (TNF-α, IL-1β, and IL-6), down-regulated the mRNA expression of TLR4 and MyD88 (P < 0.01), down-regulated the expression of TLR4, MyD88, p-ERK, p-p38, IKKβ, p-p65, p-p50 (P < 0.05), and increased the IκBα protein expression (P < 0.05). In conclusion, Chicoric acid successfully protected cow lamellar keratinocytes from LPS-induced inflammatory responses by modulating the TLR4/MAPK/NF-κB signaling pathway and downregulating inflammatory mediators.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 10
·2527-2542
·
IF 3.125
Chen Jiahui,Hou Waner,Han Bo,Liu Guanghui,Gong Jin,Li Yemeng,Zhong Danmin,Liao Qiongfeng,Xie Zhiyong
Amino acids, neurotransmitters, purines, and pyrimidines are bioactive molecules that play fundamental roles in maintaining various physiological functions. Their metabolism is closely related to the health, growth, development, reproduction, and homeostasis of organisms. Most recently, comprehensive measurements of these metabolites have shown their potential as innovative approaches in disease surveillance or drug intervention. However, simultaneous measurement of these metabolites presents great difficulties. Here, we report a novel quantitative method that uses hydrophilic interaction ultra-high-performance liquid chromatography–tandem mass spectrometry (HILIC-UPLC–MS/MS), which is highly selective, high throughput, and exhibits better chromatographic behavior than existing methods. The developed method enabled the rapid quantification of 37 metabolites, spanning amino acids, neurotransmitters, purines, and pyrimidines pathways, within 6.5 min. The compounds were separated on an ACQUITY UPLC® BEH Amide column. Serum and brain homogenate were extracted by protein precipitation. The intra- and interday precision of all of the analytes was less than 11.34 %, and the accuracy was between −11.74 and 11.51 % for all quality control (QC) levels. The extraction recoveries of serum ranged from 84.58 % to 116.43 % and those of brain samples from 80.80 % to 119.39 %, while the RSD was 14.61 % or less for all recoveries. This method was used to successfully characterize alterations in the rat brain and, in particular, their dynamics in serum. The following study was performed to simultaneously test global changes of these metabolites in a serotonin antagonist p -chlorophenylalanine (PCPA)-induced anxiety and insomnia rat model to understand the effect and mechanism of PCPA. Taken together, these results show that the method is able to simultaneously monitor a large panel of metabolites and that this protocol may represent a metabolomic method to diagnose toxicological and pathophysiological states.
Overview of experimental workflow
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 13
·18680-18688
·
IF 4.223
Sun Wenjing,Hu Xiaoyu,Xiang Yuhong,Ye Nengsheng
In this work, four kinds of covalent organic framework (COF) materials (TpPa-1, TpBD, TpDT, and TFBBD) with different pore sizes or functional groups were synthesized by an ultrasonic method for the adsorption of five sulfonamides. Optimization experiments regarding the adsorption time, vortex speed, and pH were carried out to improve adsorption efficiency. In addition, kinetic and thermodynamic experiments were conducted to explore the adsorption mechanism of the sulfonamides on the different COFs. The adsorption processes of the five sulfonamides on the four COFs fit the pseudo-second-order kinetic model and Langmuir adsorption isotherm model. Additionally, pore filling, hydrogen bond interactions, and electrostatic attraction were found to be the main adsorption mechanisms.
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Frontiers of Chemical Science and Engineering
·
2023
·Vol. 17
·期 10
·1533-1543
·
IF 4.5
Xu Shengshuo,Lu Zhenying,Wang Jinling,Huang Guangtuan,Wang Hualin,Yang Xuejing
Iron oxychloride (FeOCl) is a unique layered material with tunable electronic properties. The conventional synthetic route of chemical vapor transition involves a thermodynamics-driven gas–solid interfacial reaction which often generates macroscopic crystals with stable facets. In this study, through analyzing the effects of the synthetic parameters on the FeOCl synthesis, we discovered the dominant contribution of the α -Fe 2 O 3 precursors on the chemical property of the FeOCl product, and subsequently developed a highly-controllable synthetic route of tailoring the FeOCl structures into small sizes and exposed high-energy facets via a facile and scalable mechanical-chemical approach. The synthesized products could be systematically tuned by the ball-milling conditions of the α -Fe 2 O 3 precursors. With increased milling time, the FeOCl crystallites demonstrated reduced sizes and more exposed (110) facets. Intriguingly, these small-sized FeOCl catalysts exhibited much faster Fenton-like kinetics than the pristine macroscopic FeOCl materials. Specifically, FeOCl catalysts with a 12-hour milling time showed nearly 39 times higher efficiency toward phenol degradation than the pristine FeOCl. The structure-reactivity relationship was further elucidated using the combinatory analysis via density functional theory calculation, electron paramagnetic resonance and radical quenching probe experiments. This work provides a rationale for tailoring the surface structures of FeOCl crystallites for potential applications in environmental catalysis.
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JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
·
2019
·
IF 3.202
Xu Zhao,Jing Chen,Weiying Zhang,Chengdui Yang,Xiaoxiao Ma,Sichun Zhang,Xinrong Zhang
Lipids exert substantial influences on vertebrate embryogenesis, but their metabolic dynamics at detailed structural levels remains elusive, primarily owing to the lack of a tool capable of resolving their huge structural diversity. Herein, we present the first large-scale and spatiotemporal monitoring of unsaturated lipids with C=C specificity in single developing zebrafish embryos enabled by photochemical derivatization and tandem mass spectrometry (MS). The lipid isomer composition was found extremely stable in yolk throughout embryogenesis, while notable differences in ratios of C=C location (e.g., PC 16:0_16:1 (7) vs. 16:0_16:1 (9)) and fatty acyl composition isomers (e.g., PC 16:1_18:1 vs. 16:0_18:2) were unveiled between blastomeres and yolk from zygote to 4 h post fertilization (hpf). From 24 hpf onwards, lipid isomer compositions in embryo head and tail evolved distinctively with development, suggesting a meticulously regulated lipid remodeling essential for cell division and differentiation. This work has laid the foundation for functional studies of structurally defined lipids in vertebrate embryology.
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Journal of Coatings Technology and Research
·
2015
·Vol. 12
·期 3
·525-532
·
IF 1.298
Yi Jie,Ren Runtao,Huang Congshu,Zhang Chuyi,Ma Yupu
Poly(ether ester) polyols consisting of polypropylene glycol-2000 (PPG-2000) and different ratios of ε-caprolactone and lactic acid (LA) had been prepared by polycondensation reactions. Degradable polyurethane (PU) films were prepared with poly (ether ester) polyols and hexamethylene diisocyanate trimer. Hydrolytic degradation experiments demonstrated that PU films could be degradable in artificial seawater. Importantly, the hydrolytic degradation of PU films in artificial seawater increased with the increase of LA content. The results also showed that the surfaces of PU films were renewed and kept active, as revealed by the scanning electron microscope micrographs of degradation PU coatings. Moreover, the copper ion release rates of PU coatings prepared with poly(ether ester) polyols reached steady state at about 35 days. The degradable polyurethanes based on poly(ether ester) polyols (PPG-2000, ε-caprolactone, and LA) could be effective and durable resins for marine antifouling.
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Nano Research
·
2019
·Vol. 12
·期 9
·2335-2340
·
IF 8.515
E Bin,Huang Bolong,Zhang Nan,Shao Qi,Li Yujing,Huang Xiaoqing
Although many previous studies have shown that the shape-control of nanocrystal (NCs) is an efficient strategy to improve the catalytic performance, these syntheses were conducted under very different conditions, which are not suitable for the shape-dependent properties studies as well as catalysis optimization. Herein, we demonstrate an effective method for the selective synthesis of well-defined PtPb NCs in a highly controllable manner. Four distinct PtPb NCs, namely PtPb peanut nanocrystals, PtPb hexagonal nanoplates, PtPb octahedra nanocrystals (ONCs) and PtPb nanoparticles have been selectively prepared in the presence of different phenols. Significantly, we found that the created PtPb NCs/C shows the shape-dependent activity with the optimized PtPb ONCs/C being the most active for the ethanol reforming to H 2 , 22.4 times higher than the commercial Pt/C. The high performance of PtPb ONCs/C has been also successfully expanded into other polyhydric alcohols reformings. X-ray photoelectron spectroscopy (XPS) reveals that the high Pt(0)/Pt(II) ratio in PtPb NCs/C enhances the alcohols reforming. The density functional theory (DFT) studies show the PtPb ONCs possess the highest surface averaged electronic occupation for unit Pt-atom, matching well with XPS results. The PtPb ONCs/C also displays excellent durability with limited activity decay and negligible structure/composition changes after ten cycles. This work demonstrates an important advance in the high-level control of metallic nanostructures to tune the catalytic activities.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2020
·Vol. 38
·期 9
·921-931
·
IF 3.154
Che Kun-Ming,Zhang Ming-Zu,He Jin-Lin,Ni Pei-Hong
The structure and properties of functional nanoparticles are important for stabilizing Pickering emulsion polymerization. Recently, cellulose nanocrystals (CNCs) are increasingly favored as a bio-based stabilizer for Pickering emulsions. In this study, we reported a novel functionalized polyphosphoester-grafted CNCs for the stabilization of oil-in-water Pickering emulsions and the emulsion polymerization of styrene. First, polyphosphoester containing an amino group at one end of the chain, abbreviated as PBYP-NH 2 , was prepared by ring-opening polymerization (ROP) and hydrolysis reaction, wherein PBYP represents poly[2-(but-3-yn-1-yloxy)-2-oxo-1,3,2-dioxaphospholane]. Subsequently, CNC-COOH was obtained via 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) oxidation of CNCs. The functionalized nanocrystals CNC-PBYP-COOH with carboxyl groups and polyphosphoester on the surface were obtained by the amidation reaction of PBYP-NH 2 with CNC-COOH. Finally, we used CNC-PBYP-COOH as sole particle emulsifiers to stabilize styrene-in-water Pickering emulsions and studied its effects on the emulsions in details by using dynamic light scattering (DLS). The results indicated that the properties of these emulsions depended on the concentration of hydrophobically modified CNCs, volume ratios of oil to water, and pH values. The modified CNCs had higher ability to stabilize the styrene-in-water emulsions relative to the unmodified CNCs, and a stable oil-in-water (o/w) Pickering emulsion with diameter of hundreds of nanometers could be obtained. The resulting emulsions could be polymerized to yield nanosized latexes. The polyphosphoester-modified CNCs as green particle emulsifiers can efficiently stabilize nanoemulsions and latexes, which would promote the development of novel environmentally friendly materials.
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COLLOID AND POLYMER SCIENCE
·
2015
·Vol. 293
·期 9
·2617-2624
·
IF 1.865
Yan Zhihu,Dai Caili,Zhao Mingwei,Zhao Guang,Li Yuyang,Wu Xuepeng,Liu Yifei,Du Mingyong
Dual-stimulated wormlike micelles regulated by pH and temperature in a surface active ionic liquid system comprising of a cationic surfactant ( N -cetyl- N -methylpyrrolidinium bromide (C 16 MDB)) and an aromatic dibasic acid (pyrocatechol) were investigated. Such a system can be reversibly switched between liquid-like and gel-like states on adjusting the solution pH value. The rheology, light scattering techniques and cryogenic-transmission electron microscopy (cryo-TEM) results show the growth of spherical micelles into long and entangled wormlike micelles was responsible for the changes in flow properties such as high viscosity, shear-thinning behaviour and Maxwell-type dynamic rheology. The nuclear magnetic resonance results reveal the binding capacity of pyrocatechol to C 16 MDB is proposed as the key factor of the unusual rheological and micellar responses of this pH-stimulated system. In addition, with the increase of temperature, molecular motion of surfactant molecules becomes more active, which endow the property of thermo-stimulated to the surface active ionic liquid system.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2020
·Vol. 38
·期 7
·685-695
·
IF 3.154
Wang Sheng,Cai Si-Liang,Zhang Jie,Wan Xin-Hua
A series of novel cis poly(phenylacetylene)s (PPAs) substituted at meta -position(s) by two alkoxycarbonyl pendants, i.e. , sP-Me-C8/rP-Me-C8 , P-Me-C12 , sP-Et-C4 , sP-2C4 and sP-Oct-C4 , were synthesized under the catalysis of [Rh(nbd)Cl] 2 (nbd = norbornadiene). The dependence of elongation, screw sense, and stimuli response of helical polyene backbone on the structure of pendant, solvent, and temperature was systematically investigated in both solution and solid states. Because of n → π * interaction between vicinal carbonyl groups, sP-Me-C8/rP-Me-C8 could adopt contracted cis-cisoid helix in THF, toluene, CH 2 Cl 2 , and CHCl 3 . Such an intramolecular interaction was sensitive to the hydrogen bond donating ability of solvent and temperature, but insensitive to the dielectric constant and polarity of solvent. In poly(3-methoxycarbonyl-5-alkoxycarbonylphenylacetylene), the longer the chiral alkyl chain was, the easier the stable cis-cisoid helix could be achieved. However, when the methoxycarbonyl was changed to ethoxycarbonyl, sec -butyloxycarbonyl, and octyloxycarbonyl pendant groups, only cis-transoid helix was obtained at room temperature due to the increased steric hindrance. Moreover, lowering temperature was found to facilitate the stabilization of n → π * interactions, and reversible temperature-dependent stereomutations were achieved in sP-Me-C8 and sP-Et-C4 depending on the solvent where they were dissolved. These results suggested that the long alkyl chain, small pendant size, and lower temperature favored the stabilization of intramolecular n → π * interactions and the formation of contracted, cis-cisoid helices for poly(3,5-diester substituted phenylacetylene)s.
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Frontiers of Chemical Science and Engineering
·
2022
·Vol. 16
·期 9
·1399-1406
·
IF 4.803
Feng Xing,Du Meiqing,Wei Hongbei,Ruan Xiaoxiao,Fu Tao,Zhang Jie,Sun Xiaolong
The degradation of polymeric materials is recognized as one of the goals to be fulfilled for the sustainable economy. In this study, a novel methodology was presented to synthesize multiple highly cross-linked polymers (i.e., hydrogels) through amine-thiol scrambling under mild conditions. Amine-terminated poly(ethylene glycol) (PEG-NH 2 ) was reacted with the representative conjugate acceptors to synthesize hydrogels in organic and aqueous solutions, respectively. The materials above exhibited high water-swelling properties, distributed porous structures, as well as prominent mechanical strengths. It is noteworthy that the mentioned hydrogels could be degraded efficiently in hours to release the original coupling partner, which were induced by ethylene diamine at ambient temperature through amine-amine metathesis. The recovered PEG-NH 2 reagent could be employed again to regenerate hydrogels. Due to the multiple architectures and functions in polymeric synthesis, degradation and regeneration, a new generation of “smart” materials is revealed.
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