JOURNAL OF MOLECULAR LIQUIDS
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2024
·
IF 5.3
Faisal Ul Rehman,Amjad Iqbal,Awais Khalid,Hanna Dib,Aisha Nawaf Albalawi,Adeel Ahmed,Muhammad Usman,Mohamed A. Ismail
In this study, a highly magnetic β-cyclodextrin-modified copper ferrite (CuFe 2 O 4 @β-CD) catalyst was developed utilizing a hydrothermal method , which was subsequently utilized to degrade Levofloxacin (LEV) antibiotic in aqueous solution via heterogeneous activation of peroxymonosulfate (PMS). The findings demonstrated that the 98.87 % degradation of LEV was achieved with CuFe 2 O 4 @β-CD/PMS, much higher than that of pure CuFe 2 O 4 /PMS (87.78 %) within a 24-minute time frame under optimal parameters ([CuFe 2 O 4 @β-CD] = 0.4 g/L, [PMS] = 0.4 mM, [LEV] = 25 mg/L, pH = 6), and CuFe 2 O 4 @β-CD/PMS was present. The rate constant of CuFe 2 O 4 @β-CD/PMS (0.1608 min −1 ) was much greater than that of the CuFe 2 O 4 /PMS system (0.0822 min −1 ). The increased availability of active sites for PMS activation may be credited to the larger surface area (189.42 m 2 /g) of the CuFe 2 O 4 @β-CD catalyst in comparison to the pristine CuFe 2 O 4 (87.76 m 2 /g), which facilitated the improved degradation of LEV. Additionally, the impact of various reaction parameters and intervening anions on the degradation of LEV was investigated. The emergence of free radicals (SO 4 •− , • OH, and 1 O 2 ) was corroborated via electron paramagnetic resonance and scavenging experiments. On the basis of recognizing reaction intermediates, a hypothetical degradation mechanism for LEV was developed. PMS activation was caused by the transformation of Cu + /Cu 2+ and Fe 3+ /Fe 2+ pairs, which was accomplished via radical and non-radical pathways. Also, CuFe 2 O 4 @β-CD demonstrated exceptional stability and retained its catalytic activity after five concurrent cycles. In conclusion, the CuFe 2 O 4 @β-CD catalyst demonstrated encouraging potential in the context of purifying LEV-contaminated water.
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JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Mohan Li,Na Gan,Dian Zhou,Yi He,Fang Geng,Wei Wang,Di Wu
Aloe dairy products have occupied a certain market share, and the interaction between the small bioactive molecules contained in aloe and milk proteins during product processing profoundly affects the performance of dairy products. In this study, two typical and potentially dangerous bioactive small molecules of aloe, aloin (AA) and aloe-emodin (AE), were selected to investigate their interaction mechanism with β -lactoglobulin ( β -La). Computer simulations showed that AA/AE had the potential to form complexes with β -La, and the β -La-AA system exhibited a stronger binding affinity than the β -La-AE system. Fluorescence analysis showed that the binding affinity values of AA to β -La at 298 K were deduced to be (4.184 ± 1.06) × 10 4 M −1 , meaning medium-strength interactions, comparatively, AE to β -La was weaker, were deduced to be (3.15 ± 1.25) × 10 3 M −1 . In addition, the thermodynamic analysis found that ΔH 0 and ΔS 0 were positive in both the β -La-AA system and the β -La-AE system, which implies that the main driving force for the combination of AA/AE and β -La to form the ground state complex is the hydrophobic force. The conformation changes in β -La brought about by binding to AA/AE may alter the properties and functions of the protein, such as surface hydrophobicity and oxidation resistance. Analysis shows that β -La is potentially present in whey as an AA/AE carrier during the production of aloe milk.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2024
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IF 5.3
Xin Wang,Shurong Zhang,Xiaoyue Wang,Liping Zhou,Yang Tang,Yan Xiao,Yu Zhang,Wei Li
Advanced nanosized drug delivery systems can significantly improve efficacy and safety of first-line chemotherapeutics by enhancing tumor targeting. Herein, one-pot covalent crosslinking approach was developed to generate biodegradable tumor-targeted composite Nanogels from carboxymethyl chitosan, hyaluronic acid, cystamine and 6-ethylene-diamine-6-deoxy-β-cyclodextrin loaded with doxorubicin (DOX) for controlled intracellular DOX release. The optimized synthetic procedures generated Nanogels of about 190 nm in size and 28.3 % drug loading capability. DOX-loaded Nanogels was effectively internalized into tumor cells mainly by CD44 receptor-mediated endocytosis and rapidly released DOX in response to the high level of GSH in cytoplasm and acidic intracellular environments. DOX-loaded Nanogels significantly inhibited the tumor growth especially without appreciable side toxicities in 4 T1 tumor-bearing mice model owing to CD44 receptor-mediated active targeting and the passive targeting of Nanogels by enhanced permeation and retention effect. Overall, our newly developed composite Nanogels might be employed as a potentially effective therapeutic strategy for tumor therapy.
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PubMed
高影响因子
IMMUNOLOGIC RESEARCH
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2024
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IF 3.3
Su Xiaoran,Wang Xinliu,Zhang Xin,Sun Yajie,Jia Yugai
Massive evidence shows that intestinal tryptophan metabolites affected by intestinal flora can modulate the progression of rheumatoid arthritis (RA). However, the effects and mechanisms of intestinal tryptophan metabolites on RA are not yet detailed. Herein, we investigated the protective effects of intestinal tryptophan metabolites on RA and its detailed mechanisms. In this study, the collagen-induced arthritis (CIA) rat model was established. Based on metabolomics analysis, the contents of β-indole-3-acetic acid (IAA), indolylpropionic acid, and indole-3-β-acrylic acid in the sera of CIA rats were significantly less compared with those of the normal rats. Under the condition of Treg or Th17 cell differentiation, IAA significantly promoted the differentiation and activation of Treg cells instead of Th17 cells. Intestinal tryptophan metabolites are well-known endogenic ligands of aryl hydrocarbon receptor (AhR). Not surprisingly, IAA increased the level of Foxp3 through activating the AhR pathway. Interestingly, IAA had little impact on the level of Foxp3 mRNA, but reducing the ubiquitination and degradation of Foxp3. Mechanically, IAA reduced the expression of the transcriptional coactivator TAZ, which was almost completely reversed by either AhR antagonist CH223191 or siRNA. In vitro, IAA decreased the combination of TAZ and the histone acetyltransferase Tip60, while it increased the combination of Tip60 and Foxp3. In CIA rats, oral administration of IAA increased the number of Treg cells and relieved the inflammation. A combined use with CH223191 almost abolished the effect of IAA. Taken together, IAA attenuated CIA by promoting the differentiation of Treg cells through reducing the ubiquitination of Foxp3 via the AhR-TAZ-Tip60 pathway.
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PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Xiaoning Zhang,Haiyan Cong,Yu Sun,Haohui Li,Baoer Zuo,Meifeng Li,Sining Li,Mingyi Wang
The covalent conjugation of chitosan to β-lactoglobulin (βLg) via Maillard reaction alters its structure and characteristics, providing a method to reduce allergenicity. The molecular weight (Mw) of chitosan plays a crucial role in the conjugation process, which in turn affects the desensitization effectiveness to βLg. The degree of conjugation decreased from 46.4 % ± 2.6 % (βC-1 ) to 27.6 % ± 2.9 % (βC-100) as the Mw increased from 1 kDa to 100 kDa. Notably, βC-1 showed a significant reduction in β-sheet content, resulting in a greater increase in surface hydrophobicity and improved thermostability compared to βC-10 and βC-100. Consequently, βC-1 demonstrated the most effective desensitization, with the highest inhibition of immunoglobulin E (IgE) combining capacity and the best suppression of cytokine overproduction in KU812 cells. Additionally, the overexpression of IgE and inflammatory factors in mice was significantly suppressed after βLg was conjugated with chitosan, alleviating dysbiosis of gut microbiota at both phyla and family levels and lessening anaphylactic response to βLg. The desensitization effects were observed in the order of βC-1 > βC-10 > βC-100.
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MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING
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2019
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IF 2.722
Manoj Pudukudy,Qingming Jia,Hong Wang,Shaoyun Shan,Ramesh Rajendran
The influence of synthesis conditions on the formation of α and β polymorphs of AgVO 3 , their crystalline, structural, textual and optical properties and photocatalytic activity for dye degradation was investigated. The as-prepared polymorphs were characterized by using XRD , FTIR , EDX , SEM, TEM, HRTEM , XPS , BET and UV–vis. analyses. The analytical results indicated that a metastable α-AgVO 3 was formed in the precipitation method, whereas it was irreversibly converted into thermodynamically stable β-AgVO 3 by a hydrothermal method at 120 °C. The hydrothermal treatment was found to be decisive for the formation of β-form with high crystallinity and phase purity. The increase of hydrothermal temperature to 150 °C and 180 °C could not contributed to a significant variation in the crystalline, structural, textural and optical properties of the β-AgVO 3 . Moreover, a plausible mechanism for the phase transformation was briefly discussed. A combination of the needle and rod-like nanostructures of α form was completely changed to nanorods in β form after hydrothermal treatment. The optical band gap of α and β AgVO 3 polymorphs was found to be 1.91 eV and 1.98 eV respectively. The photocatalytic activity of the silver vanadates for the degradation of Rhodamine B indicated that β-AgVO 3 nanorods were better than α-AgVO 3 polymorphs. This could be attributed to the significant effects of its stable crystalline structure rather than specific surface area . Moreover, due to the reduced grain boundaries in β-form, their electron-hole recombination rate was lowed compared to α form. The degradation efficiency reached to 56% within 180 min under visible light irradiation. However, the increase of hydrothermal temperature does not have a significant effect in the photocatalytic activity. Moreover, the β-AgVO 3 nanorods were successfully reused for five cycles of photodegradation .
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Food Biophysics
·
2024
·Vol. 19
·期 2
·310-320
·
IF 2.8
Wu Zi-ke,Chen Ru-kang,Shi Yu,Zhu Chen-min,Wang Yi-ran,Shi Yu-gang,Ettelaie Rammile,Gu Qing
Octyl gallate (GAC8) as a bioactive compound has excellent antibacterial effectiveness, but its poor hydrophilicity limits its applications. In this work, GAC8 was encapsulated into hydroxypropyl- β -cyclodextrin (HP β CyD) cavity to form an inclusion complex (GAC8/HP β CyD-IC), and their antibacterial activities were investigated. Phase solubility test suggested that the aqueous solubility of GAC8 was prominently enhanced after forming the inclusion complex. The aqueous solution of GAC8/HP β CyD-IC yielded uniform fiber morphology with ~ 900 nm average fiber diameter. The fabricated GAC8/HP β CyD-IC nanofibers (GAC8/HP β CyD-IC NFs) were characterized by 1 H NMR, FT-IR, XRD, DSC, and TGA, revealing successful synthesis of GAC8/HP β CyD-IC NFs and the thermal stability of GAC8 was enhanced by inclusion complexation with HP β CyD. Furthermore, GAC8/HP β CyD-IC NFs possessed antibacterial activity against E. coli (12.5 mm zone of inhibition), S. aureus (18.5 mm zone of inhibition). The results of DNA and protein leakage in the experiment indicated that GAC8/HP β CyD-IC NFs can disrupt the membrane integrity of bacteria. Meanwhile, GAC8/HP β CyD-IC NFs suppressed the colony growth of E. coli on Chinese giant salamander meat. Overall, the nanofibers encapsulating GAC8/HP β CyD-IC were potential antibacterial food packaging materials.
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高影响因子
Journal of Macromolecular Science Part B-Physics
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2020
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IF 0.911
Hailin Xie,Zhihua Zhou,Wenjuan Liu,Yanmin Zhao,Tianlong Huang,Ping Chen,Ziwei Zhou,Dan Wang,Jianglong Duan,Jianjun Fang
Polymeric/ceramic composite scaffolds that are biocompatible and biodegradable are widely used for tissue engineering applications. In this work a series of poly(L-lactide-co-glycolide-co-ε-caprolactone)/nano-biaoactive glass-nano-β-tricalcium phosphate composite scaffolds were successfully fabricated and the influences of the inorganic content and freezing temperature on the physical properties were studied. The composite scaffolds with various inorganic contents showed an interconnected pore structure with irregular shapes. The composite scaffolds had a porosity that was reduced with increasing inorganic content and decreasing freezing temperature. The incorporation of inorganic fillers and decreasing freezing temperature improved the mechanical properties of the hybrid scaffolds. By appropriate control of these two factors (10.0 wt% content of NBAG and β-TCP with freezing at −30 °C) a suitable composite scaffold was prepared as a potential bone tissue engineering implant.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2017
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IF 5.401
Shulin Zheng,Hongqing Wang,Qinghua Hu,Yuyuan Wang,Jiangke Hu,Fenfen Zhou,Pengying Liu
A fluorescent sensor Hn based on β-diketone derivative for Th 4+ ions was designed and investigated, which showed high selectivity in the present of other metal ions even UO 2 2+ in CH 3 OH:H 2 O (v/v = 2:8, pH = 7.2) solution. Meanwhile, the binding mode of the sensor Hn with Th 4+ ions was found to be 1:1 with association constant of 2.46 × 10 4 M −1 . And there was linear relationship between the fluorescent intensity of Hn -Th 4+ and the concentration of Th 4+ ions covering a range of 0.06 μM–1.0 μM and the detection limit was 0.0344 μM. At the same time, the interaction properties between the sensor Hn and Th 4+ ions and the fluorescent mechanism were investigated by IR, 1 HNMR and DFT. Most importantly, this fluorescent sensor was successfully utilized for the fluorescent imaging of Th 4+ ions in living cells.
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CHROMATOGRAPHIA
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2011
·Vol. 74
·期 3
·319-325
·
IF 1.075
Shi Jie-hua,Ding Zuo-jing,Hu Ying
Enantioseparations of methyl mandelate (MMA) and methyl α -cyclohexylmandelate (MCHMA) on permethylated β -cyclodextrin (PM- β -CD) chiral stationary phase were explored in detail using high-performance liquid chromatography. The influence of the concentration of organic modifiers, along with the column temperature, was studied. In addition, the thermodynamics parameters of the enantioseparations were determined to discuss driven power in the process of enantioseparations. In addition, host−guest complexation of PM- β -CD with MMA enantiomers was simulated by quantum mechanics PM3 method for understanding the chiral recognition mechanism. The experimental results showed that the retention factor ( k ), separation factor ( α ), and resolution factor (Rs) for MMA and MCHMA resolved on the PM- β -CD column all generally decreased with the increase of methanol content, which indicated that the main chiral recognition mechanism is that the hydrophobic portions of MMA and MCHMA are included in the hydrophobic cavity of PM- β -CD to form inclusion complexes. In addition, there is an excellent linear relationship between the logarithms of retention factors ( k ) of MMA and MCHMA enantiomers and 1/ T . It was demonstrated that the enantioseparations of MMA and MCHMA on PM- β -CD chiral column were enthalpy-driven processes. The modeling results can correctly predict the retention order and provide an atomistic account of how chiral discrimination takes place. It is found that the most stable structure of ( R )-MMA/PM- β -CD complex is different with that of ( S )-MMA/PM- β -CD complex. The main driving forces responsible for chiral recognition are hydrophobic forces and weak hydrogen bondings.
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PHYTOMEDICINE
·
2026
·
IF 11.3
Yeqiu Xu,Junrui Jonathan Hai,Duan Sun,Yihan Yang,Chen Wang,Xinan Zhang,Huanli Xu,Yajin Liao,Xianjun Qu,Bo Han,Weishi Liang,Yong Hai
Background Distractive spinal cord injury (DSCI) is a severe complication of spinal deformity correction surgery, causing early disruption of the blood–spinal cord barrier (BSCB). However, the molecular mechanisms that translate mechanical stress into vascular endothelial dysfunction remain unclear, and effective pharmacological strategies targeting this process are lacking. Purpose This study aimed to investigate the therapeutic effects of sodium rutin (NaR), a water-soluble derivative of rutin, on BSCB disruption following DSCI, and to elucidate the underlying molecular mechanisms. Methods In vivo rat DSCI models and in vitro mechanical distraction models of spinal cord microvascular endothelial cells were employed. The therapeutic effects of oral NaR administration on locomotor function and BSCB permeability in rats following DSCI were systematically evaluated. Mechanistic investigations targeting the SIRT1/β-catenin axis and oxidative stress were conducted utilizing RNA sequencing, interventions with small interfering RNA and SIRT1 inhibitors, alongside standard molecular biology techniques. Results NaR treatment dose-dependently improved locomotor recovery in DSCI rats and significantly reduced BSCB permeability by restoring the expression of tight junction proteins. Mechanical distraction caused a significant downregulation of SIRT1 in endothelial cells. NaR treatment upregulated SIRT1 expression and promoted its nuclear-to-cytoplasmic translocation. This upregulation of SIRT1 was associated with significant inhibition of mitochondrial oxidative stress. Furthermore, oxidative stress was found to promote β-catenin phosphorylation and subsequent degradation via the ubiquitin-proteasome pathway. NaR stabilized β-catenin through SIRT1-mediated antioxidant effects, promoting its nuclear translocation and the downstream expression of tight junction genes. Consequently, by repairing the BSCB, NaR restricted the infiltration of immune cells (CD68 + macrophages/microglia and MPO + neutrophils) into the spinal parenchyma, thereby attenuating secondary neuroinflammation. Conclusion The SIRT1/β-catenin axis may serve as a critical mechanotransduction pathway regulating BSCB integrity. NaR protects the BSCB after DSCI by restoring SIRT1 activity, suppressing oxidative stress, and stabilizing β-catenin signaling. NaR may be a promising pharmacological strategy for the treatment of DSCI. In brief: Hai et al. identify a mechanism by which mechanical distraction disrupts the blood-spinal cord barrier through suppression of SIRT1 and oxidative destabilization of β-catenin. Oral sodium rutin restores barrier integrity, limits inflammation, and thus improves locomotor recovery, highlighting a vascular-targeted therapeutic strategy for distraction-induced spinal cord injury Download: Download high-res image (296KB) Download: Download full-size image
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FOOD HYDROCOLLOIDS
·
2026
·
IF 13.8
Hanyu Song,Bingru Li,Siyu Wu,Jingwen Xu,Baokun Qi,Shizhang Yan,Lianzhou Jiang
Soybean lipophilic protein (LP) is composed of β-conglycinin (7S), glycinin (11S), and oil body protein-phosphatidylcholine (OBPs-PC), but the interactions among its components and their effects on foaming properties remain unclear. In this study, we reconstituted these components at the native LP ratio and systematically investigated the regulatory mechanism of protein-protein interactions on foaming properties. The results showed that the nature of component interactions determined foaming properties. 7S and 11S exhibited a synergistic effect: compared with the individual components, their combination underwent conformational rearrangement, characterized by decreased particle size, transition from β-sheet to random coil, increased free sulfhydryl content, reduced surface hydrophobicity, and fluorescence quenching. These changes enhanced interfacial adsorption and foam stability, making 7S + 11S exhibit the optimal foaming ability (136.44%) and stability (58.42%). In contrast, OBPs-PC, due to its strong hydrophobicity and large-sized aggregates, inhibited interfacial adsorption, causing a significant decrease in the foaming ability of OBPs-PC-containing complexes. Molecular interaction analysis indicated that hydrogen bonds and hydrophobic interactions were the main non-covalent forces driving complex formation. Rheological results confirmed that 7S + 11S foam had the highest storage modulus and the widest linear viscoelastic region. In conclusion, 7S and 11S achieved optimal foaming properties through conformational rearrangement and synergistic interfacial behavior, while the strong hydrophobicity and large-sized aggregates of OBPs-PC disrupted interfacial equilibrium and inhibited foaming. This study provides a theoretical basis for expanding the application of LP as efficient gas-water interface stabilizers and foaming agents.
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