International Journal of Nanomedicine
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2022
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IF 7.033
Guanyu Chi,Yinghua Lv,Shuang Chao,Chenxi Hou,Yuxin Pei,Zhichao Pei
Background Multifunctional nanocarriers based on tumor targeting and intracellular monitoring have received much attention and been a subject of intensive study by researchers in recent years. In this study, we report multifunctional glyconanoparticles with activatable near-infrared probes for tumor imaging and targeted drug delivery.Methods Disulfide-functionalized dicyanomethylene-4H-pyran (DCM-SS-NH2) and amino-functionalized lactose were modified and loaded onto the surfaces of polydopamine nanoparticles (NPs) by Michael addition or Schiff-base reaction as GSH stimulation–responsive fluorescent probes and tumor-targeting moieties, respectively. Doxorubicin (DOX), a model anticancer drug, was loaded onto polydopamine through π–π interactions directly to prepare multifunctional PLDD (PDA@Lac/DCM/DOX) NPs.Results Experimental results showed that PLDD NPs had been successfully prepared. DCM, the fluorescence of which was quenched in PLDD NPs, was able to restore red fluorescence in a solution with a GSH concentration of 5 mM. The amount of DOX released from PLDD NPs was 44% over 72 hours in a weak-acid environment (pH 5). The results of CLSM and flow cytometry indicated that the PLDD NPs had good HepG2-targeting ability due to the special recognition between lactose derivative of NPs and overexpressed asialoglycoprotein receptors on HepG2 cell membrane. More importantly, the disulfide bond of DCM-SS-NH2 was broken by the high concentration of GSH inside cancer cells, activating the near-infrared fluorescence probe DCM for cancer-cell imaging. MTT assays indicated that PLDD NPs exhibited higher anticancer efficiency for HepG2 cells and had reduced side effects on normal cells compared with free DOX.Conclusion The fluorescence of modified DCM loaded onto PLDD NPs is able to be restored in the high-concentration GSH environment within cancer cells, while improving the effectiveness of chemotherapy with reduced side effects. It provides a good example of integration of tumor imaging and targeted drug delivery.
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Journal of the American Chemical Society
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2022
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IF 16.383
Yuan Tian,Zhiliang Gao,Ning Wang,Ming Hu,Yi Ju,Qiang Li,Frank Caruso,Jingcheng Hao,Jiwei Cui
Surface modification with poly(ethylene glycol) (PEGylation) is an effective strategy to improve the colloidal stability of nanoparticles (NPs) and is often used to minimize cellular uptake and clearance of NPs by the immune system. However, PEGylation can also trigger the accelerated blood clearance (ABC) phenomenon, which is known to reduce the circulation time of PEGylated NPs. Herein, we report the engineering of stealth PEG NPs that can avoid the ABC phenomenon and, when modified with hyaluronic acid (HA), show specific cancer cell targeting and drug delivery. PEG NPs cross-linked with disulfide bonds are prepared by using zeolitic imidazolate framework-8 NPs as templates. The reported templating strategy enables the simultaneous removal of the template and formation of PEG NPs under mild conditions (pH 5.5 buffer). Compared to PEGylated liposomes, PEG NPs avoid the secretion of anti-PEG antibodies and the presence of anti-PEG IgM and IgG did not significantly accelerate the blood clearance of PEG NPs, indicating the inhibition of the ABC effect for the PEG NPs. Functionalization of the PEG NPs with HA affords PEG NPs that retain their stealth properties against macrophages, target CD44-expressed cancer cells and, when loaded with the anticancer drug doxorubicin, effectively inhibit tumor growth. The innovation of this study lies in the engineering of PEG NPs that can circumvent the ABC phenomenon and that can be functionalized for the improved and targeted delivery of drugs.
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ANALYST
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2022
·Vol. 147
·期 22
·4991-4999
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IF 5.227
Jinyue Shi,Yu Lin,Weiling Qin,Mingxiang Li,Yuyi Zhou,Yeyu Wu,Hu Luo,Ke-Jing Huang,Xuecai Tan
Graphdiyne (GDY) is an sp and sp2 co-hydrocarbon allotrope whose particular structure endows it with many fascinating properties, including abundant chemical bonds, high conjugation, natural pores, high carrier mobility, high conductivity and stability, etc. In this work, two-dimensional graphdiyne is prepared as an electrode substrate material coupling with an exonuclease III-assisted amplification strategy to construct a superior-performance self-powered biosensor based on enzymatic biofuel cells for highly sensitive detection of the tumour marker miRNA-21. Glucose oxidase (GOD) is first immobilized on the GDY/AuNP composite to prepare a bioconjugate. GDY/AuNP modified carbon cloth is used as an enzyme biofuel cell electrode, which is then modified with bilirubin oxidase as a biocathode. The bioconjugate binds to GOD through specific binding to the bioanode. When miRNA-21 is present, specific recognition by exonuclease III in the system results in cleavage of the capture probe, and miRNA-21 is recovered and involved in the cycle. The target miRNA-21 then causes corresponding changes in the open-circuit voltage of the self-powered system. Based on this, a sensitive detection method was constructed, within the scope from 0.1 fM to 0.1 nM with a shallow detection limit of 55.2 aM (S/N = 3). The new approach triumphantly has been used to detect miRNA-21 in serum, which provides a compelling new way for early diagnosis of related cancers.
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Oncology Letters
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2022
·Vol. 24
·期 5
·1-12
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IF 3.111
Haoran Jiang,Xianxin Wen,Xue Zhang,Bingyu Zhang
Concanavalin A (Con A), the first and most typical representative of the legume lectin family, has a potent anti‑liver cancer effect by inducing cell apoptosis and autophagy. However, its function in human liver cancer cell migration remains unclear. The present study investigated the effect of Con A on the viability and migration of human liver cancer cells with different metastatic abilities. It was found that Con A could reduce the viability of human liver cancer cells (HCCLM3, MHCC97L and HepG2) and human hepatocytes (MIHA). In addition, Con A could inhibit human liver cancer cell migration specifically without affecting hepatocytes. Sugar inhibition assay showed that glucose‑related sugar binding sites played an important role in the inhibition of Con A on human liver cancer cell migration. In addition, Con A could affect HCCLM3 migration by activating ERK1/2, JNK1/2/3 and p38 signaling in the MAPK pathway. Moreover, Con A could regulate fibrous actin (F‑actin) redistribution and assembly via the MAPK signaling pathway. However, Con A had no significant effect on the activation of matrix metalloproteinase (MMP)‑2 and MMP‑9 in HCCLM3 cells. In conclusion, Con A may bind to glucose‑related receptor protein, activating ERK1/2, JNK1/2/3 and p38 signaling in the MAPK pathway, further contracting cells and reducing the content of F‑actin of single cells to inhibit HCCLM3 cell migration. These results would deepen the links between human liver cancer cell migration and related glycoproteins or signaling molecules, and may provide a new perspective for Con A as a potential anticancer agent.
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DYES AND PIGMENTS
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2022
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IF 5.122
Yuanyuan Zhang,Jing Wang,Wanlu Sun,Hui Yu,Shubo An,Hai-Yan Wang,Yi Liu
Increasing interest has been noted in photosensitizer design. Currently, most small molecular photosensitizers have several drawbacks, including limited penetration depth and unsatisfying photostability. For addressing these limitations, Frequency upconversion luminescence (FUCL) small molecule was adopted as a photosensitizer design strategy for its deeper penetration, sensitive imaging, and better photostability. We developed a series of FUCL (NIR-NH 2 , NIR-NHEt, NIR-NEt 2 ) molecules with rigid structures. Equipped with the most powerful electron donor , NIR-NEt 2 exhibits the highest singlet oxygen generation, the strongest upconversion luminescence intensity under 808 nm irradiation and mitochondria-targeted ability. FUCL can be a superior molecular platform for image-guided photodynamic therapy of cancer cells.
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CHEMICAL PHYSICS LETTERS
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2022
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IF 2.719
Jing Kong,Jingui Zhang,Ming Shen,Sufen Zhang,Peipei Shen,Chuanli Ren
Bacterial infection is a major cause of life-threatening infections. Zinc oxide nanoparticles (nano-ZnO) are an inorganic antimicrobial material with non-toxicity and low antimicrobial efficiency. To improve its antimicrobial activity, a series of manganese(II) oxide/ZnO nanocomposites were designed and prepared via a chemical precipitation method. After being characterized via EDS, XRD, SEM, TEM, and HRTEM, the obtained nanocomposites were proved to be successful with no impurities and exhibited smaller particle sizes. It showed improved photocatalytic activity and antibacterial activity under visible light. The MnO/ZnO-2.5% nanocomposite showed 92.3% inhibition against E. coli . MnO/ZnO nanocomposites can kill bacteria by generating reactive oxygen species.
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LASER PHYSICS
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2022
·Vol. 32
·期 12
·125201
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IF 1.38
Ruitong Zhao,Xin Wang,Peng Wang,Xingda Wu,Xianglin Fang,Yanjiao Zhang,Shaoxin Li
The real-time measurement of optical activity is of great significance for studying the chemical reactions of chiral molecules. In this study, we have built a set of linear common path optical weak measurement chiral sensor system in frequency domain, and the optical sensitivity of the system feasibly reaches 1524 for activity. Using this system, we have realized the state measurement of the ultrafast alkaline hydrolysis reaction of gluconolactone under different concentrations of catalyst. This study shows that the optical weak measurement chiral sensor can well monitor and compare the degree of hydrolysis reaction, and partly reflect the approximate change of the optical rotation in the real-time process of hydrolysis. Optical weak measurement chiral sensing does not require separation and labeling molecules, and has great application potential for the production and development of chiral compounds, especially chiral drugs.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2022
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IF 4.598
Qingyuan Song,Xueling Shan,Liyin Bu,Anna Dai,Ding Jiang,Wenchang Wang,Hiroshi Shiigi,Zhidong Chen
A highly efficient electrochemiluminescence resonance energy transfer (ECL-RET) aptasensor was constructed for ultrasensitive detection of diethylstilbestrol (DES). The composite composed of silver orthophosphate and zirconium-based metal–organic frameworks (Ag 3 PO 4 -UiO-66) was prepared as a sensing platform for aptasensor incubation. Ag 3 PO 4 , a novel ECL donor, whose emission could be obviously enhanced through loading on UiO-66. Particularly, the composite of Ag 3 PO 4 -UiO-66 could minimize the aggregation of Ag 3 PO 4 , which provided a novel possibility for the construction of high ECL efficiency sensors. UiO-66 presented ultrahigh surface area to improve the loading capacity of Ag 3 PO 4 and DES-specific aptamer. Notably, Ag 3 PO 4 with superb catalytic properties could immobilize DES aptamer via Ag–NH 2 bond and further ensured the distance between the ECL donor and the energy receptor. To design a novel ECL-RET system, GO nanosheets were synthesized as a suitable ECL acceptor which could be fixed through electrostatic adsorption. Owing to the superior spectral overlap, GO could significantly quench the ECL emission of Ag 3 PO 4 . Under the optimal conditions, the constructed ECL-RET aptasensor showed a satisfactory response to DES in a linear range of 1.0 × 10 −14 ∼ 1.0 × 10 −8 M and the detection limit was 7.2 × 10 −15 M (S/N = 3). This paper not only developed a novel approach for environmental water quality monitoring but also offered more opportunities for building highly sensitive ECL-RET aptasensors.
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NEW JOURNAL OF CHEMISTRY
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2022
·Vol. 46
·期 47
·22778-22785
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IF 3.925
Xiaozhi Li,Jutao Qiao,Dan Luo,Siyi Xu,Yuefeng Liu,Hao Liu
In this study, Ni nanoparticles were grown on a silver-coated nylon yarn (SCNY) surface by electrochemical deposition to prepare SCNY@Ni. The hierarchical Ni–Ag nanostructure promoted their synergistic effect, which enhanced the electrocatalytic behavior of glucose oxidation. The test result of SCNY@Ni showed its high sensitivity (115.26 μA mM−1 cm−2 and 67.37 μA mM−1 cm−2) with a wide linear response (from 2 μM to 1 mM and from 1 mM to 22 mM) of glucose con-centration range, a low detection limit (LOD) of 2 μM, fast response time (3 s), and good selectivity for glucose determination in the presence of sucrose (SU), aceta-mino phenol (AP), D-fructose (D-Fru), ascorbic acid (AA), dopamine (DA), hydro-quinone (HQ) and uric acid (UA) as potential interference species. Also, SCNY@Ni exhibited excellent stability, repeatability, reproducibility and flexibility. It has good prospects for large-scale production.
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POULTRY SCIENCE
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2022
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IF 4.014
Zhengtian Lyu,Haifang Li,Xin Li,Hui Wang,Hongchao Jiao,Xiaojuan Wang,Jingpeng Zhao,Hai Lin
ABSTRACT Fibroblast growth factor 23 ( FGF23 ), a bone-derived hormone, is involved in the reabsorption of phosphate ( P ) and the production of vitamin D hormones in the kidney. However, whether and how FGF23 regulates chicken bone metabolism remains largely unknown. In the present study, we investigated the effect of FGF23 on osteogenic differentiation and mineralization of chicken bone marrow mesenchymal stem cells ( BMSCs ). First, we found that the transcription of FGF23 was inhibited by β-glycerophosphate sodium ( GPS , 5 mM, 10 mM, 20 mM) and 10 −9 M 1, 25-dihydroxyvitamin D3 ( 1, 25(OH) 2 D 3 ), but was stimulated by 10 −7 M 1, 25(OH) 2 D 3 and parathyroid hormone ( PTH , 10 −9 M, 10 −8 M, 10 −7 M). Second, overexpression of FGF23 by the FGF23 adenovirus ( Adv-FGF23 ) suppressed the formation of mineralized nodules ( P < 0.001) and alkaline phosphatase ( ALP ) activity ( P < 0.05) in both differentiated and mineralized osteoblasts. Administration of FGF receptor 3 ( FGFR3 ) inhibitor (50 nM) was sufficient to restore the FGF23-decreased ALP activity ( P < 0.05), but not for the formation of mineralized nodules. In addition, the phosphorylation of ERK increased considerably with Adv-FGF23 overexpression ( P < 0.05). Administration of an ERK-specific inhibitor (10 μM) could down-regulate the phosphorylation of ERK ( P-ERK ) ( P < 0.05) and slightly restored the Adv-FGF23-reduction of ALP activity ( P = 0.08). In summary, our data suggest that GPS, 1, 25(OH) 2 D 3 , and PTH could regulate FGF23 mRNA expression in vitro. FGF23 is a negative regulator of bone remodeling. FGF23 not only inhibits BMSCs osteogenesis through the FGFR3-ERK signaling pathway but also suppresses the mineralization of mature osteoblasts.
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ACS Applied Polymer Materials
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2022
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IF 4.855
Xi Chen,Hao Zhang,Chenyao Tian,Jingzhi Jia,Hui Xie
Tremendous progress in applying small-scale magnetic robots for targeted drug delivery has sparked a growing interest among researchers. However, significant effort is needed to design and fabricate robots to meet the needs of clinical treatments, such as biocompatibility, biodegradation, and synergistic therapy. Here, we propose a multiscale magnetic hydrogel robot (MMHR) with a core–shell structure in which superparamagnetic nanoparticles and curcumin-loaded chitosan microparticles (CCMs) are encapsulated inside the core, while doxorubicin-loaded chitosan microparticles (DCMs) are embedded inside the shell. The core and shell of the MMHR are composed of pH-sensitive calcium alginate hydrogels that can alleviate the sudden release of drugs from CCMs and DCMs in gastric fluid. MMHRs can be precisely actuated to the target region through magnetic driving systems for further drug delivery. The experimental results demonstrate that the MMHR possesses superior obstacle-crossing ability and can successfully locomote on the surface of the intestine. Additionally, the co-delivery of doxorubicin and curcumin is realized through the different fabrication methods of CCMs and DCMs and shows excellent synergistic therapeutic effects against colon cancer cells. The powerful propulsion, good biocompatibility, and remarkable synergistic efficacy of the MMHR offer a promising route for active drug delivery through the gastrointestinal tract.
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ACS Applied Materials & Interfaces
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2022
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IF 10.383
Meiting Li,Yaqian Zhang,Xiaoge Zhang,Zhuoyin Liu,Junjie Tang,Miao Feng,Baizhu Chen,Dalin Wu,Jie Liu
Sonodynamic therapy (SDT) benefiting from its intrinsic merits, such as noninvasiveness and deep tissue penetrability, is receiving increasing considerable attention in reactive oxygen species (ROS)-based tumor treatment. However, current sonosensitizers usually suffer from low tumor lesion accumulation, insufficient ROS generation efficiency under ultrasound, and non-biodegradability, which seriously impede the therapeutic outcomes. Additionally, it is difficult that SDT alone can completely eradicate tumors because of the complex and immunosuppressive tumor microenvironment (TME). Herein, we simultaneously employ sonosensitive porphyrin building blocks and glutathione (GSH)-responsive disulfide bonds to construct a novel degradable multifunctional porphyrin-based hollow porous organic polymer (POP) nanosonosensitizer (H-Pys-HA@M/R), which combine SDT, “on-demand” chemotherapy, and immunotherapy. Taking the unique advantages of POPs with designable structures and high specific surface area, this H-Pys-HA@M/R nanosonosensitizer can achieve tumor target accumulation, GSH-triggered drug release, and low-frequency ultrasound-activating ROS generation with encouraging results. Furthermore, this multifunctional nanosonosensitizer can effectively evoke immunogenic cell death (ICD) response through the combination of SDT and chemotherapy for both primary and distal tumor growth suppression. Meanwhile, H-Pys-HA@M/R exhibits favorable biodegradation and biosafety. Therefore, this study provides a new strategy for reasonably designing and constructing POP-related sonosensitizers combining SDT/chemotherapy/immunotherapy triple treatment modalities to eradicate malignant tumors.
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