JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
·
2018
·Vol. 86
·期 2
·479-492
·
IF 1.745
Liu Siqi,Zhou Xiezhen,Han Weiqing,Li Jiansheng,Sun Xiuyun,Shen Jinyou,Wang Lianjun
Ambient-pressure-dried (APD) crack-free Sb-SnO 2 xerogel has been developed through a facile sol-gel approach. Propylene oxide was added into the tin and antimony precursors to cause rapid formation of the wet gel, which was then modified with silica and dried under ambient pressure. A silica modification carried out by soaking the wet gel in TEOS/ethanol solution, in combination with solvent exchange, allowed to avoid the risky and costly supercritical drying (SCD) process. The silica possessed dual-functional effects during the synthesis process as surface silylation agent and underlying silica structure. FT-IR, SAXS, TEM, and nitrogen physisorption analysis revealed a favorable silica dispersion, outstanding specific surface area (254 m 2 g −1 ), pore volume (0.52 cm 3 g −1 ), and ideal pore size (8.75 nm) for the products. The key properties of the APD crack-free Sb-SnO 2 xerogel were compared with that of SCD aerogel at the same synthesis conditions. Finally, the electrochemical behaviors of 3D electrochemical system filled with the as-synthesized materials as particle electrodes were investigated, which with regard to voltammetric charge and polarization impedance. Padding of the crack-free Sb-SnO 2 xerogels yielded a voltammetric charge 2.99 times as large as that of conventional electrochemical system, and decreased the charge transfer resistance from 46.19 to 6.39 Ω, which realize intrinsically enhanced electrochemical performance for 3D electrochemical system.
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Food Analytical Methods
·
2016
·Vol. 10
·期 5
·1592-1596
·
IF 2.167
Xie Wei-Qi,Chai Xin-Sheng
This paper reports on a practical headspace gas chromatographic method (HS-GC) for rapid determination of degree of substitution (DS) in sodium carboxymethyl starch (Na-CMS). After Na-CMS was acidified by hydrochloric acid (HCl) solution, the supernatant of the solution was added to a bicarbonate solution in a closed headspace vial. Thus, the remaining HCl reacts with bicarbonate to form carbon dioxide gas and thus was measured by HS-GC. The result showed that the Na-CMS was completely converted to H-CMS within 45 min at a room temperature when a small sample size (= 0.5 g) was used by acidification pretreatment. The present method has a good measurement precision (the relative standard deviation <3.25%) and accuracy (the relative difference < 5.46%, compared to the data measured by the reference method) on the determination of DS in Na-CMS. The present method is simple, practical, and suitable for the use in the carboxymethyl starch related research and applications.
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International Journal of Minerals Metallurgy and Materials
·
2018
·Vol. 25
·期 2
·226-235
·
IF 1.261
Chen Zhi-yuan,Bian Liu-zhen,Yu Zi-you,Wang Li-jun,Li Fu-shen,Chou Kuo-Chih
Carbon deposition on nickel powders in methane involves three stages in different reaction temperature ranges. Temperature programing oxidation test and Raman spectrum results indicated the formation of complex and ordered carbon structures at high deposition temperatures. The values of I (D)/ I (G) of the deposited carbon reached 1.86, 1.30, and 1.22 in the first, second, and third stages, respectively. The structure of carbon in the second stage was similar to that in the third stage. Carbon deposited in the first stage rarely contained homogeneous pyrolytic deposit layers. A kinetic model was developed to analyze the carbon deposition behavior in the first stage. The rate-determining step of the first stage is supposed to be interfacial reaction. Based on the investigation of carbon deposition kinetics on nickel powders from different resources, carbon deposition rate is suggested to have a linear relation with the square of specific surface area of nickel particles.
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JOURNAL OF POLYMER RESEARCH
·
2020
·Vol. 27
·期 6
·1-9
·
IF 2.426
Deng Yaling,Sun Jianjun,Ni Xingya,Yu Bo
Natural joints have the hierarchical structure of bone and cartilage, providing excellent lubrication and load-carrying capacity. In our research, the hierarchical structure artificial joints of Poly Acrylic Acid (PAA)- Poly Acrylamide (PAAm) hydrogel coating and Ti6Al4V substrate were formed using a simple yet versatile method by mimicking the hierarchical structure of natural joints. The efficient lubrication at friction interfaces was achieved. The composition and microstructure were confirmed by Fourier-transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), Scanning electron microscopy (SEM). The hydrogel coatings were successfully prepared on the surface of Ti6Al4V substrate through the adsorption of the charged group. This hierarchical structure material exhibits the lower dynamic friction, approximately 0.085, which is much lower than bare Ti6Al4V, about 0.429. The wear on the surfaces after the tribological experiment is super-shallow and has no significant fracture area around the scratch. The PAA-PAAm hydrogel has a more uniform and compact cross-linked network porous structure, and the porous size is much smaller than PAA hydrogel. The cross-linked network porous structure is the main factor accounting for the low dynamic friction. This hierarchical structure of soft and hard improve the load-carrying ability and has the potential uses in artificial joints.
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BMC GENOMICS
·
2019
·Vol. 20
·期 1
·1-19
·
IF 3.501
Lu Wei-Jia,Gao Fan-Xiang,Wang Yang,Zhang Qi-Ya,Li Zhi,Zhang Xiao-Juan,Zhou Li,Gui Jian-Fang
Background Accompanied with rapid growth and high density aquaculture, gibel carp has been seriously threatened by Carassius auratus herpesvirus ( Ca HV) since 2012. In previous study, distinct Ca HV resistances and immune responses were revealed in the diseased individuals of three gibel carp gynogenetic clones (A + , F and H). However, little is known about the gene expression changes in the survivors after Ca HV challenge, particularly their differences of innate and adaptive immune system between susceptible clone and resistant clone. Results We firstly confirmed the Ca HV carrier state in the survivors of three gibel carp clones after Ca HV challenge by evaluating the abundances of five Ca HV genes. The assay of viral loads indicated the resistant clone H possessed not only stronger resistance but also higher tolerance to Ca HV. Then, 2818, 4047 and 3323 differentially expressed unigenes (DEUs) were screened from the head-kidney transcriptome profiles of survivors compared with controls from clone A + , F and H. GO and KEGG analysis suggested that a persistent immune response might sustain in resistant clone H and F, while susceptible clone A + had a long-term impact on the circulatory system which was consistent with the major symptoms of bleeding caused by Ca HV. Among the top 30 enriched pathways of specifically up-regulated DEUs in respective clones, 26, 7 and 15 pathways in clone H, F and A + were associated with infections, diseases, or immune-related pathways respectively. In addition, 20 pathways in clone F belonged to “metabolism” or “biogenesis”, and 7 pathways involved in “circulatory system” were enriched in clone A + . Significantly, we revealed the differential expression changes of IFN system genes and immunoglobulin (Ig) genes among the survivors of three clones. Finally, myosins and Igs were identified as co-expression modules which were positively or negatively correlated to Ca HV viral loads respectively. Conclusions Our results revealed the common and distinct gene expression changes in immune and circulatory system in the survivors of three gibel carp gynogenetic clones with different Ca HV resistances. The current study represents a paradigm of differential innate and adaptive immune reactions in teleost, and will be beneficial to the disease-resistance breeding of gibel carp.
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PubMed
高影响因子
Nano Research
·
2018
·Vol. 11
·期 8
·4278-4292
·
IF 7.994
Zhang Yapei,Li Yingying,Ma Jinlong,Wang Xinyu,Yuan Zhi,Wang Wei
A major impediment in the development of chitosan nanoparticles (CTS NPs) as effective drug delivery vesicles is their rapid clearance from blood and endosome entrapment. To overcome these problems, a convenient and promising template system was developed by decorating poly(methacrylic acid) (PMAA) to the surface of 10-hydroxy camptothecin (HCPT)-loaded CTS NPs (HCPT-CTS/PMAA NPs). The results show that the presence of negatively charged PMAA significantly elongated the blood circulation time of HCPT-CTS NPs from 12 to 24 h, and reduced the blood clearance (Cl) from 30.57 to 6.72 mL/h in vivo . The calculated area under curve (AUC 0-24h ) and terminal elimination half-life ( t 1/2 ) of HCPT-CTS/PMAA NPs were 4.37-fold and 2.48-fold compared with those of HCPT-CTS NPs. Furthermore, the positively charged HCPT-CTS/PMAA NPs triggered by tumor acidic microenvironment (pH 6.5) result in a 453-fold higher cellular uptake than the negatively charged counterparts at pH 7.4. Additionally, HCPT-CTS/PMAA NPs have the ability to escape endosomal entrapment via “proton sponge effect” after incubation with HepG2 cells for 3 h at pH 6.5. Taken together, these findings open up a convenient, low-cost, but effective way to prepare HCPT-CTS/PMAA NPs as a candidate for developing vectors with enhanced long blood circulation and endosomal escape ability in future clinical experiments.
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MACROMOLECULAR RESEARCH
·
2018
·Vol. 26
·期 3
·270-277
·
IF 1.767
Ruan Zheng,Yuan Pan,Jing Titao,Xing Tao,Yan Lifeng
A novel pH-sensitive carborane and cyanine dye conjugated polypeptide and its micelles has been designed and synthesized for potential near infrared fluorescence imaging-guided boron neutron capture therapy (BNCT). The amphiphilic polymer we synthesized with tertiary amine as functional group could self-assemble to micelle nanoparticles at neutral pH, and responsive to acidic solution for the disassembly of the micelles for drug release. The tertiary amine sensitive induced process was observed via dynamic light scattering (DLS) and transmission electron microscopy (TEM). The efficiency of cell killing ability is studied by MTT assays and fluorescence microscope measurement, indicating a new intelligent system for potential NIR imaging-guided therapy.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2015
·Vol. 19
·期 11
·3347-3353
·
IF 2.446
Pan Wanjing,Liu Xiaolin,Miao Xiaowei,Yang Jun,Wang Jiulin,Nuli Yanna,Hirano Shin-ichi
A new type of hybrid battery has been assembled with magnesium metal anode, hollow MoO 2 microsphere cathode, and dual-salt electrolyte containing Mg 2+ and Li + ions. This kind of hybrid battery not only avoids metallic dendrite formation, which occurs in rechargeable lithium battery, but also ensures high-capacity intercalation reaction in the cathode, which is still a bottleneck for rechargeable magnesium battery. It is found that the morphology of MoO 2 electrode has a great effect on its electrochemical performance. The hollow MoO 2 microsphere cathode delivers an initial discharge capacity of 217.2 mAh g −1 with the coulombic efficiency of ca. 88 %. In the following cycles, its coulombic efficiency reaches nearly 100 %. In contrast, MoO 2 solid particles with a size of 1–10 μm exhibit a capacity less than 50 mAh g −1 . Inductively coupled plasma (ICP) analysis reveals that both of Mg 2+ and Li + ions take part in the cathode intercalation reaction.
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NPG Asia Materials
·
2020
·Vol. 12
·期 1
·1-16
·
IF 8.131
Qian Chunyu,Xin Tianwen,Xiao Wanshu,Zhu Huajun,Zhang Qiang,Liu Lili,Cheng Ruoyu,Wang Zhen,Cui Wenguo,Ge Zili
Electrospun fiber membranes have been extensively researched for tissue repair; however, fiber angiogenesis has been a difficult problem because the biomaterials used to prepare the fiber cannot promote angiogenesis by themselves. To endow the fiber with the function of promoting angiogenesis and oral mucosa regeneration, surface-aminated liposomes (NH 2 -LIPs) encapsulating leptin, as well as silk fiber (SF) membranes that have been immersed overnight in polydopamine (PDA) solution were synthesized. The NH 2 -LIPs were then grafted onto the surfaces of SF through reactions between the catechol groups of PDA and the amino groups of NH 2 -LIPs to induce angiogenesis on the fiber surface and thus promote oral mucosa regeneration. The PDA-modified and NH 2 -LIP-modified SF retained the original fibrous morphology but increased the SF tensile strength from 1.95 MPa to 2.87 MPa. PDA changed the hydrophilicity of the fibers and improved the adhesion of fibroblasts on the fibrous membranes. Leptin loaded onto SF via PDA had no significant effect on cell proliferation and formed 162.7-node tubes in human umbilical vein endothelial cells (HUVECs) within 10 h, which demonstrated that leptin loaded onto SF can promote angiogenesis. Wound closure of mucosa covered in leptin-loaded fibrous membranes reached 99% at 14 days postsurgery. Histologic analysis showed that leptin-loaded SFs exhibited clear stratification of new mucosa, as well as a strong CD34 signal that indicated the presence of new blood vessels and confirmed the successful loading of leptin on the fiber. Therefore, this work successfully showed that NH 2 -LIPs grafted onto the surface of SFs via PDA endow the fibers with angiogenic abilities and promote oral mucosa regeneration.
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npj Flexible Electronics
·
2022
·Vol. 6
·期 1
·1-9
·
IF 12.019
Huang Yuan,Liu Binbin,Zhang Wenyue,Qu Gangrui,Jin Shunyu,Li Xianbo,Nie Zanxiang,Zhou Hang
A conductive, elastic, and biocompatible hybrid network hydrogel was prepared by cross-linking of locust bean gum, polyvinyl alcohol, and carbon nanotubes, yielding a rough top surface and smooth bottom surface. The merging of the two pieces of hydrogel flat face to flat face forms a highly elastic hydrogel with double-rough surfaces. A piezoresistive sensor assembled with the double-rough surface hydrogel sandwiched between two carbon cloth electrodes exhibits a high sensitivity (20.5 kPa-1, 0-1kPa), a broad detection range (0.1–100 kPa) and a reliable response for 1000 cycles. The rough contact area between the hydrogels and the carbon cloth is found critical in achieving ultra-high sensitivities in the low-pressure range. Moreover, further monolithic integration of the sensor with a flexible solid-state zinc ion battery ensures the self-powering of the sensor for various human motions detection applications.
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3 Biotech
·
2021
·Vol. 11
·期 7
·1-9
·
IF 2.406
Chang Siyuan,Pan Xin,Zhao Mingzhe,Li Guoqing,Wang Xue,Fan Yachen,Song Wei,Li Bingfeng,Zhang Sen,He Xuejun
Glycosylation is a prominent biological mechanism, affecting the structural and functional diversity of many natural products. In this study, a novel thermostable uridine diphosphate-dependent glycosyltransferase gene PpGT1 was cloned from Paenibacillus polymyxa NJPI29 and recombinantly expressed in B. subtilis WB600. The purified PpGT1 had a molecular weight of 45 kDa, as estimated using SDS-PAGE. The PpGT1 could catalyze the glycosylation of vanillic acid, methyl vanillate, caffeic acid, cinnamic alcohol, and ferulic acid. Moreover, PpGT1 possessed good thermostability and retained 80% of its original activity even after 12 h of incubation at 45 °C. In addition, PpGT1 remained stable within a neutral to alkaline pH range as well as in the presence of metal ions. The synthesis of methyl vanillate 4- O -β-D-glucoside by purified PpGT1 reached a yield 3.58 mM in a system with pH 8.0, 45 °C, 12 mM UDP-Glc, and 4 mM methyl vanillate. 3D-structure-based amino acid sequence alignments revealed that the catalytic residues and C-terminated PSPG motif were conserved. These unusual properties indicated that PpGT1 is a candidate UGT for valuable natural product industrial applications.
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PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 19
·27677-27687
·
IF 5.19
Long Yujiao,Li Hongna,Jin Hongmei,Ni Jinren
The chemical and biological mechanisms of electrochemical elimination of Microcystis aeruginosa ( M. aeruginosa ) using boron-doped diamond (BDD) anode were comparatively explored in three different electrolytes (chloride, sulfate, and phosphate solutions). The most efficient elimination of M. aeruginosa was observed in chloride solution, which was attributed to the greatest total long-lived oxidants from the favorable formation of active chlorine. Moreover, the high permeability of active chlorine resulted in profound intracellular damages to chlorophyll- a , microcystin-LR (MC-LR), superoxide dismutase (SOD) enzyme, and DNA in the chloride system. The change of membrane permeability and degradation of the released MC-LR induced by active chlorine were further confirmed by the increase of extracellular MC-LR in the initial 5 min and a complete decay in the subsequent 15 min, while the change in morphology of algae cells was insignificant from SEM images. In sulfate and phosphate electrolytes, membrane damages were much more pronounced based on lipid peroxidation observation, although changes in cell morphology was found more significant in phosphate system. The higher concentrations of oxidants (·OH, O 3 , H 2 O 2 , S 2 O 8 2− ) generated in sulfate than in phosphate solution explained the greater efficiency of electrochemical elimination of M. aeruginosa in the sulfate electrolyte in terms of changes of cell density, OD 680 , chlorophyll- a , MC-LR, lipids, SOD enzyme, and DNA.
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