SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Yunhao Li,Shuhao Wang,HengYu Li,Dandan Liu,Yan Jin,Guodong Kang,Yiming Cao
Lithium extraction from salt-lake brine with a high Mg 2+ /Li + ratio is crucial for mitigating lithium supply shortage. Positively charged polyamide nanofiltration membranes, which are often prepared by performing interfacial polymerization (IP) using polyethyleneimine and trimesoyl chloride, are considered to be promising candidates for Mg 2+ /Li + separation owing to their high energy efficiency and low environmental impact. However, the performance of these membranes is severely hindered by “useless pores” and “defects” present in the flexible polyamide layer. Herein, we provide a facile strategy for modifying flexible semi-aromatic polyamide chains by intercalating a rigid electropositive monomer, histamine, to remold the internal pores and positive charge density at a molecular level. Two routes, namely, “swelling-embedding-reacting-shrinking (SERS)” and “in-situ interposition (SIP)” were used to construct a new polyamide layer with rigid–flexible microstructures. The fabricated SERS-0.50 membrane showed a 5-fold higher water flux and 2-fold increase in the Mg 2+ /Li + separation factor compared with the IP membrane. Moreover, the SIP-0.15 membrane with an enhanced positive charge density exhibited a considerably higher Mg 2+ /Li + separation factor (15.38, 500 % increase compared with that of the IP membrane) for brine with a Mg 2+ /Li + ratio of 20. This study provides a reference for developing nanofiltration membranes used for high-efficiency Mg 2+ /Li + separation.
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DYES AND PIGMENTS
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2022
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IF 5.122
Niuniu Cui,Jiawei Li,Ziwen Xie,Xiaofei Yan,Dongming Qi
The colouration of cotton fabrics through the charge attractions between cationic nanoscale pigment and cotton fabrics, is a novel pigment dyeing technology with the advantages of no salt, recyclable dyebath, high uptake, and good wet fastness. In this study, the cationic copolymer nanoparticle dispersants with a diameter of about 32 nm were synthesized by reversible addition-fragmentation chain transfer (RAFT) alcoholic dispersion polymerization . The cationic nanoparticle-based nanoscale pigment dispersion was prepared, and its colloidal and dyeing properties were also investigated. The results indicated that the nanoscale pigment dispersion had good dispersion performance in the aqueous medium with relatively narrow distribution and excellent colloid stability against the external effects of centrifuge, freeze-thaw test, and pH, in which the cationic nanoparticles were indeed adsorbed on the surface of anionic C.I. pigment red 170 particles by electrostatic attraction . It was found that the salt-free cationic nanoparticle-based nanoscale pigment dyed cotton fabrics acquired higher coloring intensity and colour fastness due to establishing the charge attractions between nanoscale pigment and cotton. More importantly, the dyebath is recyclable for circular dyeing, as a result significantly reduces the discharge of dyeing effluent, which provides a viable and promising strategy for ecologically sustainable dyeing technology for cotton fabrics.
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PHARMACOLOGICAL RESEARCH
·
2022
·
IF 10.334
Ying Zhang,Changjun Yin,Chao Wei,Shuai Xia,Zhu Qiao,Xue-Wen Zhang,Bin Yu,Jun Zhou,Rong Wang
Migration, invasion, epithelial-mesenchymal transformation (EMT), and chemotherapeutic resistance are the leading causes of therapeutic failure in people with colorectal cancer (CRC). The migration of exosomal miRNA between cancer cells and the tumor microenvironment is directly associated with malignant behavior in cancer-associated fibroblasts (CAFs). In the context of earlier research, the purpose of the current study was to assess the role and potential mechanism of miR-625-3p released by CAFs in CRC cells. Exosomes were extracted and purified from CAFs conditioned medium by ultracentrifugation. Western blot , immunohistochemistry, CCK-8, transwell assay, H&E staining, Tunnel, real-time PCR, double luciferase assay, RNA-binding protein immunoprecipitation (RIP), and immunofluorescence double staining experiments were used to investigate the effects of CAFs-Exo and miR-625-3p on CRC cell invasion, migration, proliferation, EMT, chemotherapeutic resistance, and molecular mechanisms. The current results indicated that CAFs-Exo was directly internalized by CRC cells, and exosomal miR-625-3p derived from CAFs might promote migration, invasion, EMT and chemotherapeutic resistance in CRC cells by inhibiting the CELF2/WWOX pathway, providing a potential candidate for CRC prediction and treatment.
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高影响因子
BIOMATERIALS
·
2022
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IF 15.304
Yan Gao,Zilin Song,Li Jia,Yi Tang,Chengcheng Wang,Xiuli Zhao,Haiyang Hu,Dawei Chen,Mingxi Qiao
Despite the important role of reactive oxygen species (ROS) in battling cancer, ROS production with current approaches has been severely limited by the deficiency of oxy-substrates in tumor microenvironment . Herein, an atorvastatin (Ato)-catalytic self-amplified approach was utilized for sustainable ROS production and enhancing anti-tumor efficacy of PD-L1 silencing. A C 18 -pArg 8 -ss-pHis 10 lipopeptide based self-assembled nanoplexes was developed to co-encapsulate AMP-activated protein kinase (AMPK) activator of Ato and PD-L1 siRNA. Efficient delivery of payloads was achieved because of the acidic pH triggered the protonation of pHis 10 , disulfide-bond exposure for cleavage and subsequent cytosolic translocation. Ato was found to activate AMPK, boosting the highly restrained mitochondrial fatty acid oxidation (FAO) in cancer cells for ROS production. The ROS derived from FAO further activated AMPK, creating a positive-feedback mechanism of sustainable ROS production. The self-amplified ROS production from cellular mitochondrial FAO was maintained by the sufficient intracellular fatty acid substrates arising from the dysregulated lipid metabolism and Ato inhibited triglyceride synthesis in cancer cells. The excessive ROS level was found to successfully induce immunogenic cell death effect, boosting the anti-tumor efficacy of PD-L1 silencing. Overall, the Ato catalyzed self-amplified ROS production has been demonstrated as a promising alternative for cancer therapy.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Erfei Dong,Ting Chen,Min Fang,Weiju Zhu,Cun Li
In this paper, water-soluble cysteamine (CA)-capping CdSe quantum dots (CA-CdSe) could be used as a continuous fluorescent sensor. The CA-CdSe QDs can respond to Ag + with a detection limit of 54.1 nM. Interestingly, CA-CdSe quantum dots combined with Ag + to generate a new nano-fluorescence sensor-Ag + modified CA-CdSe QDs (Ag + @CA-CdSe). Ag + @CA-CdSe can detect glutathione (GSH) with good sensitivity and anti-interference performance. The detection limit of Ag + @CA-CdSe fluorescenct sensor for GSH is as low as 0.74 μM. In addition, the novel nano-fluorescent sensor Ag + @CA-CdSe exhibited good cell permeability and was successfully applied to detect exogenous and endogenous GSH concentrations in cells. It could distinguish cancerous and normal cells by in vitro cell fluorescence imaging.
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高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 11
·1-15
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IF 6.408
Qin Dongmiao,Meng Shuo,Wu Yusheng,Luo Zhi,Deng Biyang
An efficient electrochemiluminescence resonance energy transfer (ECL-RET) method is proposed which combines the luminescent materials of tris(4,4′-dicarboxylicacid-2,2′-bipyridyl) ruthenium(II) (energy donor) and tin dioxide and tin disulfide quantum dots (SnO 2 /SnS 2 QDs) (energy acceptor) into the isoreticular metal − organic framework-3 (IRMOF-3) material to form a composite. In this mode, the distance between the energy donor and the acceptor was greatly shortened, reducing the energy loss, and thereby effectively improving RET efficiency and further significantly improving the ECL signal. The obtained composite (SnO 2 /SnS 2 QDs-Ru@IRMOF-3) was combined with sandwich immunoreaction to construct an ECL immunosensor for the sensitive detection of procalcitonin (PCT). Under the optimized experimental conditions with a working potential of − 1.48 V (vs Ag/AgCl), the proposed PCT biosensor exhibited a linear concentration range of 1 × 10 −4 –200 ng mL −1 , with a detection limit of 0.029 pg mL −1 ( S / N = 3). The biosensor was used to detect PCT in actual samples. The biosensor has broad application prospects in biological analysis and clinical diagnosis due to its high sensitivity, good selectivity, and good stability. Graphical abstract
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Photonics
·
2022
·Vol. 9
·期 10
·784
·
IF 2.536
Zixin Yang,Gaozhe Cai,Jianlong Zhao,Shilun Feng
Urine examinations are widely applied in hospitals using urine test strip analyzers or other sophisticated professional instruments. However, such methods are inconvenient health monitoring of patients at home. Herein, we construct an optical device for point-of-care testing (POCT) for urine analysis at home or on the spot. A black box and color calibration curve are established to eliminate the influence of ambient light with an independent internal lighting system included in the device. A Raspberry Pi with a CSI camera is programmed to automatically collect the strip images and identify the HSV values of the image with an image processing algorithm. During this process, these corrected colors are converted to concentration values by preloaded standard curves. Under optimal conditions, the proposed POCT device can quantitatively and automatically detect glucose within 1 min, with linear detection ranging from 2 mM to 60 mM and a detection limit of 1.16 mM. In addition, the device demonstrates satisfactory accuracy and quantitative analysis of ketone bodies, glucose, protein, occult blood, pH, and leukocytes in human urine samples with high-resolution concentrations, achieving results similar to those obtained with hospital instruments. The proposed device is portable and user-friendly, providing convenient colorimetric analysis for urine. Furthermore, the proposed device also has considerable potential for the development of in vitro diagnosis methods through combination with other test strips.Keywords:colorimetry detection;HSV parameters;point-of-care testing;urine test
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DYES AND PIGMENTS
·
2022
·
IF 5.122
Xiaoqing Xiong,Ye Yuan,Gengwen Chen,Miao Li,Lihua Lyu,Hongjuan Zhao,Jun Yan,Yulan Qian,Junyang Zhu,Chengqi Jiao
The investigation on the construction strategy, relationship between the structure and photophysical properties of fluorescein analogs plays an important role in the rational design of specific fluorescent dyes. In this work, a fluorescein analog Fc-1 , containing a reactive aldehyde group was prepared using 2′, 7′-dichlorofluorescein (DCF) as the raw material and Vilsmeier–Haack reaction mechanism. Meanwhile, two more fluorescein analogs Fc-2 and Fc-3 were synthesized based on Fc-1 and the Knoevenagel reaction mechanism. The results of Fc-1 indicated that the yield of Fc-1 was higher than 95% after a step of the low-temperature hydrolysis (40 °C) from an intermediate imine salt DCF-3 . The fluorescence quantum yield ( Φ ) of Fc-1 was relatively high in polar protic solvents, even in H 2 O ( Φ = 0.25). Fc-1 had large Stokes shifts in different solvents (≧ 123 nm), which was indentified by the Gaussian calculation of Fc-1 . Meanwhile, Fc-1 was also used as a ratio and colorimetric fluorescent probe for H 2 S in vitro , and the dianion ( [Fc-1-2H] 2- ) mechanism for detecting H 2 S was proposed by nuclear magnetic resonance (NMR), pH titration, and Gaussian calculation. Additionally, Fc-1 can be used in leukocyte classification because of its relatively high Φ in water, cell permeability, and cytotoxicity. All the data of dyes Fc-2 and Fc-3 indicated that the derivatization of the carboxyl group for Fc-2 did not affect the photophysical properties of Fc-2 . Both of them had relatively large Stokes shifts and long emission wavelengths . The large Stokes shift of Fc-2 and Fc-3 was due to the distribution of electron clouds in the excited states of Fc-2 and Fc-3 . The results of pH titration proved that dyes Fc-2 and Fc-3 were sensitive to the changes in pH and exhibited their potentials as pH probes. Thus, the strategy of constructing fluorescent dyes based on Vilsmeier–Haack and Knoevenagel reactions and the relationship between structure and properties will be beneficial to developing more dyes with large Stokes shift.
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Frontiers in Pharmacology
·
2022
·
IF 5.988
Huang Jianbo,Wang Hong,Huang Lei,Zhou Yuqing
Background: Lipid and polymer microbubbles (MBs) are widely used as ultrasound contrast agents in clinical diagnosis, and possess great potential in ultrasound-mediated therapy due to their drug loading function. However, overcoming the limitations of stability and echo enhancement of MBs are still a considerable challenge.Methods: A series novel block, graft and block-graft copolymers was proposed and prepared in this work, and these copolymers were used as shells to encapsulate perfluoropentane as ultrasound contrast agents. First, block, graft and block-graft copolymers with different topological structures were prepared. Then, these copolymers were prepared into block copolymer phase-transition MBs, graft copolymer phase-transition MBs, and block-graft copolymer phase-transition MBs, respectively. Finally, the dexamethasone was used for drug-loaded phase-transition microbubbles model to explore the potential of theranostic microbubbles.Results: Finally, these three resulting copolymer MBs with average size of 4–5 μm exhibited well enhancement of ultrasound imaging under the influence of different frequencies and mechanical index, and they exhibited a longer contrast-enhanced ultrasound imaging time and higher resistance to mechanical index compared with SonoVue in vitro and in vivo. In vitro drug release results also showed that these copolymer MBs could encapsulate dexamethasone drugs, and the drug release could be enhanced by ultrasonic triggering. These copolymer MBs were therapeutic MBs for targeted triggering drug release.Conclusion: Therefore, the feasibility of block, graft, and block-graft copolymers as ultrasonic contrast agents was verified, and their ultrasonic enhancement performance in vitro and in vivo was compared. The ultrasound contrast agents developed in this work have excellent development potential in comprehensive diagnosis and treatment.
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DYES AND PIGMENTS
·
2022
·
IF 5.122
Hongrui Cheng,Song Chen,Min Li,Yongfeng Lu,Haixin Chen,Xiao Fang,Haijiang Qiu,Wensong Wang,Cheng Jiang,Yuanhui Zheng
Room temperature phosphorescence (RTP) materials have motivated massive attention due to their great advantages in anti-counterfeiting and optical encryption. However, the commonly used carbon-based RTP materials suffer from drawbacks of photobleaching, quenching and single phosphorescence color outputs, which obstruct their performance in high-level anti-counterfeiting techniques. Herein, we present a universal method for the fabrication of a [email protected] ( [email protected] 2 ) composite that possesses ultralong lifetime, high phosphorescence quantum efficiency (PQE), excellent luminescence stability, and dual-color phosphorescence emission. Taking advantage of the restriction by rigid network of SiO 2 and stable covalent bonding between CDs and SiO 2 , the triplet excited states of CDs are stabilized. As a result, the [email protected] 2 exhibit ultralong lifetime of 1.33 s and 0.38 s under excitation at 254 nm and 365 nm, respectively. Furthermore, the phosphorescence color of [email protected] 2 can change from cyan to yellow when the exciting wavelength switches from 254 nm to 365 nm. The obtained [email protected] 2 also exhibit outstanding characteristics of anti-photobleaching and phosphorescence stability in an aqueous solution. Based on the unique phosphorescent properties of the [email protected] 2 , a stable and dual-color phosphorescence security label is designed for advanced information encryption.
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Food Chemistry-X
·
2022
·
IF 6.443
Qingxia Yuan,Kunling Lv,Jinwen Huang,Shujing Sun,Ziyu Fang,Hongjie Tan,Hong Li,Dan Chen,Longyan Zhao,Chenghai Gao,Yonghong Liu
Grey mangrove ( Avicennia marina (Forssk.) Vierh.) fruit is a traditional folk medicine and health food consumed in many countries. In this study, its polysaccharides (AMFPs) were obtained and analyzed by chemical and instrumental methods, with the results indicating that AMFPs consisted of galactose, galacturonic acid, arabinose, and rhamnose in a molar ratio of 4.99:3.15:5.38:1.15. The dynamic changes in AMFPs during the digestion and fecal fermentation processes were then investigated. The results confirmed that AMFPs were not depolymerized by gastric acid and various digestive enzymes. During fermentation, 56.05 % of the AMFPs were utilized by gut microbiota. Galacturonic acid, galactose, and arabinose from AMFPs, were mostly consumed by gut microbiota. AMFPs obviously decreased harmful bacteria and increased some beneficial microbiota, including Megasphaera , Mistuokella , Prevotella , and Megamonas . Furthermore, AMFPs obviously increased the levels of various short-chain fatty acids. These findings suggest that AMFPs have potential prebiotic applications for improving gut health.
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MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Siyi Li,Die Gao,Jing Lin,Zeng Wen,Kailian Zhang,Zhining Xia,Dandan Wang
A double-network hydrogel was prepared with the chitosan-cellulose composite as the rigid network and polyacrylamide as the flexible network (abbreviated as CCP hydrogel). A deep eutectic solvent synthesized by choline chloride and malic acid was used as the assistant solvent to dissolve cellulose for preparation of the CCP hydrogel. The obtained CCP hydrogel was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermal gravimetric analysis, and stress-strain test. The properties of the CCP hydrogel are obviously better than those of the single network hydrogel. The compressive strength of CCP hydrogel is 2.8 times that of the chitosan-cellulose single network hydrogel. The CCP hydrogel exhibits the favorable swelling performance (swelling ratio of 955 %–1377 %), water-locking ability (water retention value of 40 %), and stability in aqueous system. Moreover, the CCP hydrogel performs the excellent adsorption capacity, fast adsorption efficiency, and good selective towards tetracyclines. The effects of temperature and solution pH on the adsorption properties of CCP hydrogel were assessed. The Langmuir, Freundlich, pseudo-first-order, pseudo-second-order, and thermodynamic models were applied to explore the adsorption characteristics of CCP hydrogel. After multiple adsorption/desorption cycles, the CCP hydrogel still maintains 84.3 %–93.4 % of adsorption capacity. Finally, the CCP hydrogel combined with ultra-performance liquid chromatography-mass spectrometry were used to analyze tetracyclines in food samples. The tetracycline (0.14 μg/mL) and minocycline (0.080 μg/mL) in milk, tetracycline (0.082 μg/mL) and minocycline (0.089 μg/mL) in fish, and tetracycline (0.078 μg/mL) and demeclocycline (0.10 μg/mL) in chicken were detected by CCP-hydrogel method with a high accuracy (recoveries of 75.82 %–108.8 %). Furthermore, for comparison with the CCP-hydrogel method, the traditional precipitation method was also applied to detect the tetracyclines in foods. The CCP-hydrogel method possesses a higher accuracy than traditional method, and can more accurately and satisfactorily detect tetracyclines in foods.
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