BRAIN RESEARCH BULLETIN
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2023
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IF 3.8
Jingwei Zhao,Mingming Zhang,Huamin Zhang,Ying Wang,Bingyu Chen,Jing Shao
Diosmin is a flavone glycoside with a confirmed therapeutic effectiveness on the chronic venous disorders. In this paper, the classical mouse depression model induced by LPS was established to explore the effect of Diosmin on depression. Firstly, we found that Diosmin could inhibit the inflammation and neuronal damage in the prefrontal cortex (PFC) of mice, and thus alleviating the LPS-induced depressive-like behaviors. Specifically, Diosmin treatment significantly suppressed the secretion of pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β), reduced the activation of microglia, and inhibited the expression of NLRP3 inflammasome and its downstream effector caspase-1 in both PFC of mice and BV2 microglial cells exposed to LPS. Then, we demonstrated that pretreatment with Diosmin dramatically suppressed the LPS-induced oxidative stress in the PFC of mice, manifested in the decrease of reactive oxygen species and malondialdehyde while increase of catalase activity. Consistently, Diosmin also alleviated the oxidative stress in BV2 cells exposed to LPS. Finally, we confirmed that Diosmin effectively suppressed the activation of NF-κB signaling pathway in the PFC of LPS-treated mice. Further in vitro experiments also verified that Diosmin could prevent the p65 transposition to nucleus in LPS-treated BV2 cells, suggesting that the antidepressant effects of Diosmin are partially mediated by blocking of NF-κB signaling. Taken together, this study proposes the potential antidepressant effect of Diosmin, which provides useful support to the development of new therapies for depression.
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Advanced Healthcare Materials
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2023
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IF 10
Xiaomeng Li,Shuaibing Liu,Shanshan Han,Qingqing Sun,Jianmin Yang,Yuhang Zhang,Yongchao Jiang,Xiaofeng Wang,Qian Li,Jianglin Wang
Biomechanical stimuli derived from the extracellular matrix (ECM) extremely tune stem cell fate through three-dimensional (3D) and spatiotemporal changes in vivo. The matrix stiffness as a crucial factor during bone tissue development is dynamic stiffening. However, most in vitro models to study the osteogenesis of mesenchymal stem cells (MSCs) are static or stiffening in a 2D environment. Here, we create a dynamic and controllable stiffening 3D biomimetic model to regulate the osteoblastic differentiation of MSCs with a dual-functional gelatin macromer that can generate a double-network hydrogel by sequential enzymatic and light-triggered crosslinking reactions. Our findings showed that these dynamic hydrogels allowed cells to spread and volume expansion prior to secondary crosslinking and to sense high stiffness after stiffening. The MSCs in the dynamic hydrogels, especially the hydrogel stiffened at the late period, presented the significantly elevated osteogenic ECM secretion, gene expression, and YAP/TAZ nuclear localization. In vivo evaluation of animal experiments further indicated that the enhancement of dynamic stiffening on osteogenesis of MSCs substantially promoted bone remodeling. Consequently, this work reveals that the 3D dynamic stiffening microenvironment as a critical biophysical cue not only mediates the stem cell fate in vitro, but also augments bone restoration in vivo. This article is protected by copyright. All rights reserved
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JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
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2023
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IF 6.1
Yiqian Zhu,Hui-Fang Xiao,Meisheng Li,Shouyong Zhou,Haq Navaz,Ailian Xue,Chao-Nan Wang,Rong Xu,Dawei Yang,Chenglung Chen,Yijiang Zhao
It has been a longstanding problem to combat the oxidation process of MXene membranes. Here, we propose a strategy to construct nano bridge defect repair loose nanofiltration membrane with the help of MXene oxidation. The oxidation kinetics of Ti 3 C 2 T x was investigated in both model and experimental aspects. The acid solution leads to aggregation and smaller d-spacing. However, the oxidation of MXene was accelerated in alkaline solution via reaction with hydroxyl anions resulting in a hydrophilic MXene membrane surface. The water permeability of the pH=12-Ti 3 C 2 T x membrane increased to a maximum value of 26.5 L·m -2 ·h -1 ·bar -1 together with a high rejection (98%) of Janus green B (JGB). Titanium dioxide, which was in situ formed by oxidation of MXene, gives the membrane self-cleaning properties under UV light. The water recovery ratio was as high as 96.6%. To further study the mechanism of in situ oxidation of MXene to form nano-titanium dioxide, the oxidation stages were analyzed which revealed that the in-situ oxidation process involved three different oxidations stages. This research offers a new perspective on MXene membrane design, paving the way for a new method of in situ nano bridge defect repair loose MXene nanofiltration membrane.
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BMC Complementary Medicine and Therapies
·
2023
·Vol. 23
·期 1
·1-14
·
IF 3.9
Han Wenrun,Zhang Dandan,Zhang Peng,Tao Qianqian,Du Xiaoli,Yu Chunquan,Dong Pengzhi,Zhu Yan
IntroductionLiver X Receptor (LXR) agonists could attenuate the development of atherosclerosis but bring excess lipid accumulation in the liver. Danlou Recipe was believed to be a benefit for improving the lipid profile. Thus, it is unclear whether Danlou Recipe could attenuate hyperlipidemia without excess lipid accumulated in the liver of mice. This study aimed to clarify if Danlou Recipe could alleviate the progression of hyperlipidemia in mice without extra lipids accumulated in the liver.Methods Male murine macrophage RAW264.7 cells and murine peritoneal macrophages were used for the in vitro experiments. Cellular cholesterol efflux was determined using the fluorescent cholesterol labeling method. Those genes involved in lipid metabolism were evaluated by qRT‐PCR and western blotting respectively. In vivo, a mouse model of hyperlipidemia induced by P407 was used to figure out the effect of Danlou Recipe on reverse cholesterol transport (RCT) and hyperlipidemia. Ethanol extract of Danlou tablet (EEDL) was prepared by extracting the whole powder of Danlou Prescription from ethanol, and the chemical composition was analyzed by ultra-performance liquid chromatography (UPLC).Results EEDL inhibits the formation of RAW264.7 macrophage-derived foam cells, and promotes ABCA1/apoA1 conducted cholesterol efflux in RAW264.7 macrophages and mouse peritoneal macrophages. In the P407-induced hyperlipidemia mouse model, oral administration of EEDL can promote RCT in vivo and improve fatty liver induced by a high-fat diet. Consistent with the findings in vitro, EEDL promotes RCT by upregulating the LXR activities.Conclusion Our results demonstrate that EEDL has the potential for targeting RCT/LXR in the treatment of lipid metabolism disorders to be developed as a safe and effective therapy.
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INDUSTRIAL CROPS AND PRODUCTS
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2023
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IF 5.9
Ningning Zhang,Lili Yang,Yongpeng Ma,Jinyong Huang,Shixin Zhu,Hao Dong,Zhuang Zhao,Yang Lu
Cornus officinalis Siebold & Zucc. is an important Chinese herbal medicine with traditional clinical applications, a long history of cultivation and high economic value in China. C. officinalis is distributed mainly in the Shaanxi, Henan, and Zhejiang provinces of China, which are also historically the main production areas, and which still provide 90% of the medicinal material of this species for the contemporary market. In this study, we investigated the main germplasm resources of C. officinalis across its distribution, based on fruit morphology, the concentrations of selected medicinally active ingredients, and molecular population genetic analysis. The results indicated that the C. officinalis populations sampled had abundant variation in fruit morphology and chemical component content, and showed high genetic diversity , as revealed by microsatellite markers. Clustering results based on morphology, active ingredient concentration and microsatellite markers supported the idea that populations from Zhejiang province were distinct from those in Shaanxi and Henan. The fruit and molecular data suggested that the Shaanxi and Henan populations were mixed, which could be attributed to their close geographical distance and frequent germplasm exchange . Most of the C. officinalis populations had relatively weak genetic differentiation from others sampled, and most of the individuals sampled showed extensive admixture. We suggest that artificial gene flow caused by intensive cultivation and widespread trading is responsible for the observed admixture of genetic components and blurred genetic boundaries between C. officinalis populations, especially in the Shaanxi and Henan populations. We also propose some suggestions for the efficient utilization and conservation of C. officinalis germplasm. These findings provide valuable information on the genetic resources of C. officinalis and offer guidelines for breeding programs and scientific management of C. officinalis .
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Nanoscale
·
2023
·Vol. 15
·期 20
·9049-9059
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IF 6.7
Xiaolong Deng,Shanshan Wang,Yi Liu,Jiafeng Cao,Jinzhao Huang,Xingwei Shi
In this study, a hierarchical interconnected porous metal sulfide heterostructure was synthesized from CoFeAl layered double hydroxides (LDHs) by a two-step hydrothermal process (sulfidation and a NaOH etching process). Among the as-made samples, the CoFeAl-T-NaOH electrode exhibited excellent oxygen and hydrogen evolution reaction catalytic activities with overpotentials of 344 mV and 197 mV at the current density of 100 mA cm−2, respectively. Meanwhile, small Tafel slopes of 57.7 mV dec−1 and 106.5 mV dec−1 for water oxidation and hydrogen evolution were observed for the CoFeAl-T-NaOH, respectively. Serving as both the cathode and anode for overall water splitting, the CoFeAl-T-NaOH electrode reached a current density of 10 mA cm−2 at a cell voltage of 1.65 V with excellent stability. The enhanced electrocatalytic activity could be attributed to: the hierarchical interconnected nanosheet structure facilitating mass transport; the porous structure promoting electrolyte infiltration and reactant transfer; the heterojunction accelerating charge transfer; and the synergistic effect between them. This study offered a new clue for in situ synthesizing porous transition-metal based heterojunction electrocatalysts with a careful tuning of the sequence of sulfuration and alkaline etching to enhance the electrocatalytic performance.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Siyuan Liu,Jingxing Liu,Zefen Wang,Zhiqi Wu,Yiliang Wei,Pengru Liu,Xiongdiao Lan,Yexin Liao,Ping Lan
Glucose oxidase (GOx) has a great application potential in the determination of glucose concentration. However, its sensitivity to the environment and poor recyclability limited its broader application. Herein, with the assistance of DA-PEG-DA, a novel immobilized GOx based on amorphous Zn-MOFs (DA-PEG-DA/ [email protected] /PDA) was developed to impart excellent properties to the enzyme. SEM, TEM, XRD, and BET analyses confirmed that GOx was embedded in amorphous ZIF-7 with ∼5 wt% loading. Compared with free GOx, DA-PEG-DA/ [email protected] /PDA exhibited enhanced stability, excellent reusability, and promising potential for glucose detection. After 10 repetitions, the catalytic activity of DA-PEG-DA/ [email protected] /PDA can maintain 95.53 % ± 3.16 %. In understanding the in situ embedding of GOx in ZIF-7, the interaction of zinc ion and benzimidazole with GOx was studied by using molecular docking and multi-spectral methods. Results showed that zinc ions and benzimidazole had multiple binding sites on the enzyme, which induced the accelerated synthesis of ZIF-7 around the enzyme. During binding, the structure of the enzyme changes, but such changes hardly affect the activity of the enzyme. This study provides not only a preparation strategy of immobilized enzyme with high activity, high stability, and low enzyme leakage rate for glucose detection, but also a more comprehensive understanding of the formation of immobilized enzymes using the in situ embedding strategy.
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JOURNAL OF ETHNOPHARMACOLOGY
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2023
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IF 5.4
Qin Tang,Zhengcai Ma,Xiang Tang,Yan Liu,Huimin Wu,Yu Peng,Baihua Jiao,Rui Wang,Xiaoli Ye,Hang Ma,Xuegang Li
Ethnopharmacological relevance Helicobacter pylori (H. pylori) is a major pathogen colonized in the human stomach and is implicated in gastritis, peptic ulcer, and gastric carcinoma. Antibiotics are useful for eradicating H. pylori but failed for drug resistance, making it urgent to develop effective and safe drugs. Rhizoma Coptidis was reported as one of the most effective Chinese medicines to treat H. pylori -related gastrointestinal diseases, while the precise antimicrobial mechanism remains unclear. Thus, it is of great significance to study the antimicrobial ingredients and corresponding mechanisms of Rhizoma Coptidis. Aim of the study To search for the most effective alkaloid against H. pylori in Rhizoma Coptidis and illustrate the probable mechanisms . Materials and methods Five main alkaloids in Rhizoma Coptidis were isolated. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were tested to determine the most effective one. Bacterial growth experiments, Annexin V-FITC/PI staining, TUNEL staining, and transmission electron microscopy (TEM) were performed to further study the anti- H. pylori activity of coptisine (Cop) . The in vivo effect of Cop on H. pylori eradication rate and H. pylori -induced inflammation was investigated in mice . Transcriptomics was used to understand the underlying mechanism of eradicating H. pylori and reducing host inflammation. Western blot, RT-PCR, and ELISA experiments were utilized and confirmed that cagA was one of the targets of Cop. Results According to the MIC and MBC, Cop was the most effective alkaloid against H. pylori , especially with no drug resistance developed . In vitro experiments showed that Cop inhibited H. pylori by inducing DNA fragmentation, phosphatidylserine exposure, and membrane damage. Cop (150 mg/kg/day) effectively eradicated H. pylori in mice and reduced the levels of IL-2 and IL-6 to relieve gastric inflammation. Transcriptomic analysis revealed that virulence factor cagA was one of the hub genes associated with the inflammation-improving effect of Cop. That is, Cop could decrease the expression of CagA and subsequently reduce the translocation of CagA to gastric epithelial cells, thereby improving the morphology of hummingbird-like phenotype induced by CagA and alleviating inflammation . Conclusions Cop is the most effective alkaloid in Rhizoma Coptidis and might act through multiple mechanisms for H. pylori eradication along with reducing the expression of CagA to alleviate inflammation.
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FOOD CHEMISTRY
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2023
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IF 8.8
Chao Zhang,Zichen Zheng,Kewei Liu,Marc Debliquy,Qiaoquan Liu
The palatability of cooked rice is susceptible to the flavor and effective detection of volatile organic compounds (VOCs) can avoid deterioration and improve the taste quality. Herein, hierarchical antimony tungstate (Sb 2 WO 6 ) microspheres are synthesized through a solvothermal process and the effect of solvothermal temperature on the room temperature gas-sensing properties of gas sensors is investigated. Outstanding sensitivity towards VOC biomarkers (nonanal, 1-octanol, geranyl acetone and 2-pentylfuran) in cooked rice is achieved and the sensors exhibit remarkable stability and reproducibility, which are contributed to the formation of the hierarchical microsphere structure, larger specific surface area, narrower band gap and increased oxygen vacancy content. The kinetic parameters combined with principal component analysis (PCA) effectively distinguish the four VOCs while the enhanced sensing mechanism was substantiated through density functional theory (DFT) calculation. This work provides a strategy for fabricating high performance Sb 2 WO 6 gas sensors which can be practically applied to food industry.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 17
·3581-3592
·
IF 4.3
Zhou Qian,Su XiaoLu,Yao YanXing,He Bin,Jin FengMei,Gao MengYue,Wang Qi,Feng GuiPing,Li BaoXi,Liu Rui,Dong Jun
Enrichment for the detection of quinolone residues is usually cumbersome and requires large amounts of toxic organic reagents. Therefore, this study synthesized a low-toxicity hydrophobic deep eutectic solvent (DES) with dl -menthol and p-cresol, which was then characterized by Fourier transform infrared spectroscopy, nuclear magnetic resonance, and thermal analysis. A simple and rapid vortex-assisted liquid–liquid microextraction method was developed based on this DES for the extraction of eight quinolones from cattle urine. The optimal extraction conditions were screened by examining the DES volume, extraction temperature, vortex time, and salt concentration. Under the optimal conditions, the linear ranges of the eight quinolones were 1 ~ 100 μg/L with good linearity ( r 2 was 0.998 ~ 0.999), and the limits of detection and quantification were 0.08 ~ 0.30 μg/L and 0.27 ~ 0.98 μg/L, respectively. The average extraction recoveries of spiked cattle urine samples were 70.13 ~ 98.50% with relative standard deviations below 13.97%. This method can provide a reference for the pre-treatment of quinolone residue detection. Graphical Abstract
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CARBON
·
2023
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IF 10.9
Junyu Lu,Haoruo Zhang,Mushan Yuan,Xiaochuang Di,Long Ni,Yinfu Luo,Yang Chen,Huawei Zou
The application of microwave absorption (MA) materials in realistic aerospace scenarios would be difficult without a reliable mechanical carrier. However, achieving broad effective absorption bandwidth (EAB) of the aramid honeycomb at low weight gain is still a significant challenge. Herein, we achieved rapid in-situ flowering of RGO with 3D distribution in honeycomb holes by optimizing the hydrophilic thermal (HDT) N 2 H 4 ·H 2 O reduction process, making full use of the internal space of the honeycomb. The synergy of super-broadband MA and ultra-light in aramid honeycomb can be achieved by filling the 3D macro-dispersed flower-like RGO without other media. In the frequencies of 2–18 GHz, the minimum reflection loss (RL min ) of −48.38 dB was obtained in a 3% (w/w) RGO-filled honeycomb at 14.80 GHz, with a thickness of 5.2 mm. Moreover, the 3% (w/w) RGO-filled honeycomb composites achieve a broad EAB of 13.84 GHz at the thickness of 9.0 mm with only 3% increase in weight. Actual and simulated MA performance verifies the uniformity and reliability of the foamed RGO-filled honeycomb composites, where the power loss of the composites is mainly based on the conductive losses and multiple polarization from the macroscopic distribution of RGO. The electromagnetic parameters of the foamed RGO-filled honeycomb can precisely be controlled through the weight gain of GO impregnation. Compared to ordinary impregnated coatings with high weight gain, this study provides a simple and effective pathway for preparation of MA honeycomb structures with weight gain less than 4%, which have a significant potential in the field of aerospace stealth.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2023
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IF 5.6
Yu Bai,Mi Tian,Ping He,Yongzhe Zhang,Jie Chen,Zixia Zhao,Jingsi Lan,Beiru Zhang
Prolonged renal inflammation contributes to fibrosis, which may eventually lead to irreversible chronic kidney disease. Our previous work demonstrated that LIM and cysteine-rich domain 1 (LMCD1) are associated with renal interstitial fibrosis in a 21-day unilateral ureteral obstruction (21UUO) mouse model. Interestingly, based on the gene expression omnibus database, we found that LMCD1 is enhanced in the mouse kidney as early as 5, 7, and 10 days following unilateral ureteral obstruction (UUO), suggesting that LMCD1 may exert its function in an earlier phase. To validate this conjecture, a 7UUO mouse model and a tumor necrosis factor-α (TNF-α)-stimulated HK-2 cell model were established, followed by injection of adenovirus vectors carrying short hairpin RNA targeting LMCD1. LMCD1 silencing ameliorated renal collagen deposition and reduced the expression of profibrotic factors in the 7UUO model. LMCD1 silencing alleviated tubulointerstitial inflammation by mitigating F4/80 + cell infiltration, monocyte chemoattractant protein-1 release and nuclear factor-κB activation. In addition, LMCD1 silencing suppressed NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome activation and nuclear factor of activated T cells 1 (NFATc1) nuclear translocation. Consistent results were obtained in TNF-α-stimulated HK-2 cells in vitro . Mechanistically, the transcriptional coactivator LMCD1 cooperates with the transcription factor NFATc1 to increase NLRP3 expression. Collectively, these findings suggest that LMCD1 participates in tubulointerstitial inflammation via an LMCD1-NFATc1/NLRP3 mechanism. LMCD1 may therefore become a potential target for the control of renal inflammation and fibrosis.
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