BIOLOGICAL & PHARMACEUTICAL BULLETIN
·
2023
·Vol. 46
·期 6
·764-772
·
IF 2
Xin Zhang,Zhifang Liang,Ying Zhou,Fang Wang,Shan Wei,Bing Tan,Yujie Guo
Diabetic peripheral neuropathy (DPN) is an early developing complication of diabetes mellitus associated with nerve dysfunction. Artesunate (ART), a natural compound extracted from the herb Artemisia annua L., was reported to benefit neural injury. However, whether ART has a role in preventing DPN is still unknown. In this study, a rat model of DPN with a high fat diet feeding and streptozotocin (STZ) injection was established. The findings indicated that ART treatment significantly ameliorated hyperglycemia-induced hot plate reaction latency (HPRL) decline, cold sensitivity and mechanical allodynia, and nerve injury by inhibiting sciatic nerve apoptosis. Further, ART restored high glucose (HG)-induced elevated apoptosis and deficient survival in rat neuronal Schwann cells, RSC96 cells. We demonstrated that ART promoted protein kinase B (AKT) phosphorylation as well as its downstream factor mammalian target of rapamycin (mTOR) in vivo and in vitro. Of note, the protective effects of ART in RSC96 cells under HG condition could be counteracted by LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor. Taken together, ART mitigated hyperglycemia-induced nerve injury by suppressing apoptosis and promoting the viability of Schwann cells via the PI3K/AKT/mTOR signaling pathway.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2023
·
IF 22.1
Xiaofei Liu,Tianxing Wang,Yilin Chen,Jingtao Wang,Wenjie Xie,Rongqian Wu,Xingtao Xu,Lihui Pang,Xiaogang Zhang,Yi Lv,Guangzhao Wang,Yusuke Yamauchi,Tian (Leo) Jin
Orbital hybridization is a powerful tool for modulating electronic structures toward various electrocatalytic reactions. Compared to the widely reported d - d hybridization in d -block metal alloys, the p - d orbital hybridization between d -block metals and p -block elements could provide new opportunities for regulating the electronic properties and thus promoting the electrocatalytic activities. Herein, we report a distinctive p -block metalloid-regulating p - d orbital hybridization to realize the fabrication of RuSb nanobranches for bifunctional hydrogen evolution (HER) and hydrazine oxidation (HzOR), which exhibits huge potential in overall hydrazine splitting (OHzS) by utilizing thermodynamically favorable HzOR instead of sluggish oxygen evolution on the anode. To our delight, RuSb delivers an impressively low overpotential of 39 mV for HER and 252 mV for HzOR at 10 mA cm −2 in alkaline seawater. The two-electrode OHzS electrolyzer with RuSb||RuSb couple can achieve outstanding electrocatalytic activity with an extraordinarily small cell voltage of 35 mV to drive 10 mA cm −2 in alkaline seawater, outperforming the Pt/C||Pt/C couple under the same condition. Density functional theory calculations further indicate that the Sb doping can not only mediate the adsorption energy for hydrogen but also the energy barrier for the dehydrogenation of *N 2 H 3 . Therefore, our work verifies the huge potential of p - d orbital hybridization for the development of a bifunctional OHzS system in alkaline seawater.
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Yan Liu,Shukun Tang,Xu Wang,Xiaoliang Wang,Xianghua Tang,Qian Wu,Zunxi Huang,Junmei Ding
The long-term and excessive use of pyrethroid pesticides poses substantial health risks and ecosystem concerns. Several bacteria and fungi have been reported that could degrade pyrethroids . The ester-bond hydrolysis using hydrolases is the initial regulatory metabolic reaction of pyrethroids. However, the thoroughly biochemical characterization of hydrolases involved in this process is limited. Here, a novel carboxylesterase, designated as EstGS1 that could hydrolyze pyrethroid pesticides was characterized. EstGS1 showed low sequence identity (<27.03%) compared to other reported pyrethroid hydrolases and belonged to the hydroxynitrile lyase family that preferred short short-chain acyl esters (C2 to C8). EstGS1 displayed the maximal activity of 213.38 U/mg at 60 °C and pH 8.5 using p NPC2 as substrate, with K m and V max were 2.21 ± 0.72 mM and 212.90 ± 41.78 µM/min, respectively. EstGS1 is a halotolerant esterase and remains stable in 5.1 M NaCl. Based on molecular docking and mutational analysis, the catalytic triad of S 74 -D 181 -H 212 and three other substrate-binding residues I 108 , S 159 , and G 75 are critical for the enzymatic activity of EstGS1. Additionally, 61 and 40 mg/L of deltamethrin and λ-cyhalothrin were hydrolyzed by 20 U of EstGS1 in 4 h. This work presents the first report on a pyrethroid pesticide hydrolase characterized from a halophilic actinobacteria.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Ning Ding,Ziwei Li,Lin Jiang,Hong Liu,Yanping Zhang,Yingxue Sun
Performic acid (PFA) has been increasingly used in wastewater disinfection due to its strong oxidizing ability and few disinfection byproducts. However, its disinfection pathways and mechanisms towards pathogenic bacteria disinfection are poorly understood. In this study, E. coli , S. aureus , and B. subtilis were inactivated using sodium hypochlorite (NaClO), PFA, and peracetic acid (PAA) in simulated turbid water and municipal secondary effluent. Cell culture-based plate counting showed that E. coli and S. aureus were extremely susceptible to NaClO and PFA and achieved a 4-log inactivation at CT s ≤ 1 mg/L·min with an initial disinfectant concentration of 0.3 mg/L. B. subtilis was much more resistant. At the initial disinfectant dose of 7.5 mg/L, PFA required CT s of 3–13 mg/L·min to achieve a 4-log inactivation. Turbidity negatively affected the disinfection. In the secondary effluent, the CT s required for PFA to achieve a 4-log inactivation of E. coli and B. subtilis were 6–12 times higher than those required in simulated turbid water, and a 4-log inactivation of S. aureus could not be achieved. PAA showed a much weaker disinfection ability than the other two disinfectants. The reaction pathways of E. coli inactivation by PFA included both direct and indirect reactions, in which the PFA molecule accounted for 73 %, and ·OH and peroxide radicals accounted for 20 % and 6 %, respectively. During PFA disinfection, E. coli cells were severely disintegrated, while the S. aureus cell exteriors remained mostly intact. B. subtilis was the least affected. Compared with cell culture-based analysis, the inactivation detected by flow cytometry was significantly lower. Viable but non-culturable bacteria after disinfection were believed to be primarily responsible for this inconsistency. This study suggested that PFA was able to control regular bacteria in wastewater, but it should be used with caution when treating recalcitrant pathogens.
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JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 13
·2201057
·
IF 3.1
Shadi Ali Hassen Ahmed,YeTong Yang,Shuming Li,Haobin Shi,Linghui Qian,Jing Zhang
The ability to extract peptides and proteins from biological samples with excellent reusability, high adsorption capacity, and great selectivity is essential in scientific research and medical applications. Inspired by the advantages of core-shell materials, we fabricated a core-shell material using amino-functionalized silica as the core. Benzene-1,3,5-tricarbaldehyde and 3,5-diaminobenzoic acid were used as model organic ligands to construct a shell coating by alternately reacting the two monomers on the surface of silica microspheres. The resultant material featured an outstanding capability for the adsorption of cationic peptides, most likely owing to its porous structure, a large number of carboxylic functional groups, and low mass-transfer resistance. The maximum saturated adsorption capacity reached 833.3 mg/g and the adsorption process took only 20 min. Under optimized adsorption conditions, the core-shell material was used to selectively adsorb cationic peptides from the tryptic digestive solution of lysozyme and bovine serum albumin, Specifically, the analysis results showed seven cationic peptides in the eluate and twenty anionic peptides in the supernatant, which indicates the efficient trap of most cationic peptides in the digestive solution.
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ENVIRONMENTAL TOXICOLOGY
·
2023
·Vol. 38
·期 7
·1756-1767
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IF 4.5
Lin Zhao,Liyuan Wang,Han Wang,Tao Xi,Sijia Liu,Yang Li,Jianping Ruan,Yongqing Huang
Fluoride induces endoplasmic reticulum (ER) stress in ameloblasts, which is responsible for enamel mineralization disorder. Fluoride induces autophagy in ameloblasts, but the molecular mechanisms through which ameloblasts respond to fluoride-induced cellular stress and autophagy remain unclear. This study investigated ER stress-induced autophagy and the regulatory role of the ER molecular chaperone GRP78 in fluoride-induced autophagy in ameloblast LS8 cells. To explore the relationship between fluoride-induced ER stress and autophagy, we assessed changes in fluoride-induced autophagy in LS8 cells following overexpression and/or silencing of the ER stress molecular chaperone GRP78. We found that autophagy induced by fluoride was further increased after GRP78 overexpression in LS8 cells. Fluoride-induced autophagy was reduced in GRP78-silenced LS8 cells. Furthermore, we found that ER stress can regulate autophagy in fluoride-treated ameloblasts (LS8 cells) and that the GRP78/IRE1/TRAF2/JNK pathway is involved in the underlying regulation. Our study suggests that ER stress plays a role in fluoride-induced damage by inducing ameloblast autophagy.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Jiong Wang,Zhibin Liu,Zhirong Sun
In this study, a graphite felt (GF) electrode material supporting a Cu-based catalyst (CuHDC-400/GF) was prepared by a hydrothermal process and subsequent heat treatment. A heterogeneous electro-Fenton (EF) system was built to investigate the degradation of sulfamethoxazole (SMX) by the composite cathode. Scanning electron microscopy, X-ray diffraction, Raman spectroscopy , and X-ray photoelectron spectroscopy were used to characterize the physical and chemical properties of the composite cathode. The results show that the composite cathode CuHDC-400/GF has excellent catalytic performance, achieving 100 % removal of SMX (10 mg/L) within 45 min (aeration rate = 0.6 L min −1 , current density = 5 mA cm −2 , initial pH = 5.6). Furthermore, efficient removal of SMX can be achieved over a wide pH range (pH = 3–9). The electrode material can still efficiently degrade SMX after eight cycles. Compared with a heterogeneous EF system, in which the Cu catalyst was directly added to the reaction solution, using CuHDC-400/GF as the cathode realizes the in-situ catalysis of H 2 O 2 , leading to reduced catalyst consumption and enhanced electrode stability. The investigation of potential SMX degradation pathways and mechanisms revealed that the continuous production of active free radicals is facilitated by the redox cycle between Cu Ⅰ and Cu Ⅱ on the electrode surface, which can enable SMX degradation. Unlike the Fe-based Fenton reaction, the active superoxide radical O 2 – was identified as the major contributor to SMX degradation in this system, followed by the hydroxyl radical OH. The active sites for SMX degradation were predicted by density functional theory calculations. Combined with the measured mass/charge ratios, the possible degradation paths and intermediates of SMX were predicted. Furthermore, toxicity analysis shows that toxicity significantly declines after degradation. This work is expected to inspire subsequent research on heterogeneous EF cathode materials with high catalytic performance.
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Materials Express
·
2023
·Vol. 13
·期 1
·81-84
·
IF 0.7
Wang Wen-Wen,Yang Li-Jie,Guo Jun-Feng
Pancreatic cancer is a common tumor of the digestive system with a high degree of malignancy and poor prognosis. Its incidence has significantly increased worldwide in recent years. Currently, approximately 90% of patients cannot be treated surgically. Thus, drug therapy plays an important
role in pancreatic cancer treatment. In this study, hydrogels loaded with mitomycin were prepared from hyaluronic acid and carboxymethyl chitosan, which have excellent biocompatibility. SEM results showed that the hydrogels had a three-dimensional interoperable porous structure, and CCK-8
results showed that hydrogels loaded with mitomycin significantly reduced cancer-cell proliferation. The hydrogels also reduced the migration of cancer cells in a dose-dependent manner, indicating their potential use as a drug-carrier material for pancreatic cancer treatment.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Xianliang Wu,Huijuan Liu,Xuetao Guo,Zhenming Zhang,Jiachun Zhang,Xianfei Huang
An increasing number of researchers have focused on microplastics (MPs) pollution in inland freshwater lakes due to its ecotoxicity, while little is known about the effects of hydrological periods on MPs distribution. Therefore, this study aims to investigate MPs distribution, morphological characteristics and physicochemical indices in various environments in dry and wet periods in Caohai Lake. The results exhibited that cultivated soil, water, and sediment in Caohai Lake have been polluted by MPs in dry and wet periods. There were pellets, fragments, film, and fibers of MPs in both dry and wet periods, and MP foam was additionally found in the wet period. MPs with 0 to 0.5 mm possessed the largest proportion in the five environments in dry and wet periods, followed by MPs with 0.5 to 1 mm and 1 to 5 mm. In Caohai Lake, the black, white, green, red, and transparent MPs in dry period, and black, colourful, grey, red and transparent MPs in wet period were found. The developed structural equation model confirmed that MPs in sediment were probably mainly from soil. There are negative effects of the relative abundance of MPs from cultivated soil to lake water in the dry period, whereas the opposite is true in the wet period. Interestingly, the complex and fast water velocity in the estuary in the wet period led to a lower relative abundance of MPs in its sediment in comparison with the dry period. The distribution model of MPs in estuary and lake water in dry and wet periods is not inconsistent. Our results suggest that the related government department should take measures to reduce the MPs pollution in Caohai Lake, especially from the source.
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RESEARCH ON CHEMICAL INTERMEDIATES
·
2019
·Vol. 45
·期 9
·4315-4334
·
IF 2.064
Xun Suhang,Yu Zhendong,He Minqiang,Wei Yanchen,Li Xiaowei,Zhang Ming,Zhu Wenshuai,Li Huaming
In order to effectively catalyze the oxidation of dibenzothiophene (DBT) in diesel, a phosphotungstic-based ionic liquid (IL) supported catalyst [C 16 mim] 3 PW 12 O 40 /TiO 2 was prepared through a one-step method. Commercially available TiO 2 ( C -TiO 2 ), a type of transition metal oxide, was selected as a carrier for comparison. The structure and composition of the catalysts were studied through TG, FT-IR, UV-DRS, wide-angle XRD, N 2 adsorption–desorption, SEM and XPS analysis. It was found that the Keggin structure of [C 16 mim] 3 PW 12 O 40 was still preserved in the supported catalysts. In addition, the catalysts were applied in extractive coupled catalytic oxidative desulfurization (ECODS) system with H 2 O 2 participating, and dibenzothiophene (DBT) could be totally removed after 1 h under optimum conditions. In addition, factors that affected the desulfurization efficiencies were also been studied in detail. The oxidation product after the reaction was detected by means of GC–MS analysis, DBTO 2 ( m / z = 216.0) was proved to be the only product of DBT and the possible mechanism was proposed. Moreover, the reusability of the supported ionic liquid catalyst was also investigated, and the oxidation of DBT could still reach 97.8% after eight cycles.
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CANCER CHEMOTHERAPY AND PHARMACOLOGY
·
2019
·Vol. 84
·期 3
·527-537
·
IF 3.008
Weng Qi,Zhou Leilei,Xia Lihua,Zheng Yixin,Zhang Xiangli,Li Fengzhi,Li Qingyong
Purpose Recent researches are attempting to verify that the 3D cell models can provide a gap bridge between in vitro and in vivo for anticancer drug evaluations. The aim of this study was to continue the development of novel in vitro 3D cell models and the investigation of the cellular uptake mechanism of camptothecin (CPT) and its derivatives [FL118 (10,11-methylenedioxy-20( S )-camptothecin), 9-Q20 (9- p -trifluoromethylphenyl-10,11-methylenedioxy-20( S )-camptothecin)] in 2D and 3D cell models. Methods The 3D cell models were established using ultralow attachment 96-well plates. The cytotoxicity of CPT, FL118, and 9-Q20 was evaluated by MTT method. The effects of compound concentration, incubation time, temperature, and transporter inhibitors on the cellular uptake of CPT, FL118, and 9-Q20 were examined in 2D and 3D cell models. Results The cytotoxicity of CPT, FL118, and 9-Q20 was lower in 3D cell models than 2D cell models. In 2D Caco-2 cell model, the uptake rate of CPT, FL118, and 9-Q20 was faster during the early time of incubation. In 3D Caco-2 cell model, the uptake capacity of CPT, FL118, and 9-Q20 was significantly improved over time. In 3D Caco-2 cell model, Verapamil (P-gp inhibitor) and Gefitinib (BCRP inhibitor) more significantly increased the uptake of CPT and 9-Q20. In contrast, P-gp and BCRP did not affect the accumulation of FL118 in 2D and 3D Caco-2 cell models. The accumulation of CPT, FL118, and 9-Q20 was greater in HepG2 cells than HCT116 cells. Conclusion The 3D cell models provided more potency information on the process of cellular uptake of CPT, FL118, and 9-Q20, which more objectively reflected the drug sensitivity and drug resistance in vivo compared with the 2D cell models.
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Soft Matter
·
2019
·Vol. 15
·期 11
·2319-2327
·
IF 3.399
Na Qi,Hui Zhao,Qiaozhi Wang,Yan Qin,Hao Yuan,Ying Li
Quantum dots (QDs) are of great interest due to their excellent fluorescence properties and thus, they have been widely studied. Compared with the typical organometallic synthetic routes and hydrothermal methods usually carried out under high temperatures, methods employing colloidal templates can be used for preparing QDs in mild conditions and have gained increasing attention. In this prospect, a hydrogel is an ideal colloidal template for the preparation of QDs in an aqueous medium, while the related study for in situ preparation of QDs in a gel and the consequent functionalization of QDs are in demand. In this paper, we proposed a two-step method to prepare CdS QDs in a sodium alginate (SA) gel, which showed effective constraint in the uniform size distribution of QDs. Without the introduction of additional ligands, the prepared CdS–SA QDs exhibited responsiveness to pH and detection of Fe3+, thus providing a simplified way for the functionalization of QDs. CdS–SA QDs showed good biocompatibility and stability in a certain concentration, which indicated the prospective applications of CdS–SA QDs in the fields of biological labeling and environmental sensing.
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危险品化学品经营许可证（带存储）