LANGMUIR
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2016
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IF 3.993
Junyong Sun,Sufan Wang,Feng Gao
Special functionalization of semiconducting polymer dots (Pdots) is highly desired to expand their applications in chemo/biosening. Herein, carboxyl-functionalized poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(1,4-benzo-{2,1′,3}-thiadiazole)] dots covalently tagged with aminated β-cyclodextrin (NH2–CD) have been designed to construct a ratiometric sensor for cholesterol (Cho). Using CD-Pdots as energy donors with rhodamine B (RB) as energy acceptors, a fluorescence resonance energy transfer (FRET) pair has been built because the host–guest interaction between RB and CD attached to Pdots brings donors and acceptors into close proximity. In the presence of Cho, the acceptors will depart from the donors because of the competitive inclusion interaction between Cho and RB with CD, resulting in the hindering of the FRET process between CD-Pdots and RB. On the basis of the turn-on fluorescence of CD-Pdots and turn-off fluorescence of RB, a sensitive ratiometric method for the determination of Cho in the concentration range from 25 to 350 nM with a detection limit of 4.9 nM was achieved. The proposed method was validated to determine free Cho in human serum samples with satisfactory results.
研究领域:
材料科学
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分析化学
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纳米技术
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生物传感器
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高分子化学
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Journal of the Iranian Chemical Society
·
2023
·Vol. 20
·期 5
·1165-1178
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IF 2.4
Xie Xueqiao,Huang Wenrui,Chen Tong,He Xingcheng,Han Juan,Wang Yun,Zhou Yuxi
The deep eutectic solvents (DESs) + K 2 HPO 4 aqueous two-phase systems were prepared. The phase separation capacity of betaine–glycerol became weakened with the increase in temperature, followed by choline chloride–glycerol and betaine–glycol (Bet–EG). However, the temperature did not have a significant effect on the biphasic region for ChCl–EG. β-glucosidase with the tag of choline-binding protein (LytA) was constructed and expressed successfully, and purified by APTSs with enhanced enrichment based on the affinity adsorption between choline salt and choline-binding protein. In the ATPS composed of 23.11% Bet–EG and 40.50% K 2 HPO 4 , the recombinant β-glucosidase (GLytA) has the best purification effect with a recovery rate of 89.07% and purification factor of 8.3359. Furthermore, when the purified GLytA was mixed with cellulase to catalyze microcrystalline cellulose, the saccharification rate was up to 81.2%. The activity of the mixed enzyme did not decrease significantly in the 8 days. There was still 64.22% residual activity in the mixture after 35 days. The purified recombinant enzyme by a single step can be used for subsequent catalysis or preservation directly, which has the potential to applicate in industry.
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Coatings
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2023
·Vol. 13
·期 2
·422
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IF 3.4
Haoyang Ni,Haiwei Fu,Hongbiao Wang,Hongwen Zhang,Yifan Zhu,Xiuqing Fu
This study aimed to improve the surface hardness and wear resistance of spindle hook teeth with special shapes to reduce the cost of replacing the spindle on cotton pickers. For this goal, a Ni-Co-β-SiC composite coating with different concentrations of β-SiC nanoparticles (0, 1, 2, 3, and 4 g/L) was electrodeposited on the surface of spindle hook teeth. The hardness, elemental composition, and micromorphology of the spindle hook teeth were characterized by microhardness tests, an energy spectrum analyzer, and a scanning electron microscope after cutting with the spindles. The actual wear process of the coating was determined by wear simulation and scratch wear tests, and the effect of the concentration of β-SiC nanoparticles on the properties of the coating was investigated. The results show that Ni-Co-β-SiC composite coating has a typical cellular structure. The hardness first increases and then decreases, and the wear resistance (including friction coefficient, scratch area, and shape of wear area) first decreases and then increases, mainly due to the pinning role and agglomeration of β-SiC nanoparticles. When the concentration of β-SiC was 1 g/L, the hardness reached a maximum of 506.2 HV0.1, the coefficient of friction reached a minimum of 0.13, and the wear area and wear micromorphology reached the most suitable values. Therefore, this Ni-Co-β-SiC composite coating had the best microhardness and wear resistance.Keywords:spindle hook teeth;β-SiC nanoparticles;Ni-Co-β-SiC composite coatings;wear;microhardness;electrodeposition
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REACTIVE & FUNCTIONAL POLYMERS
·
2022
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IF 4.966
Lijie Liu,Yaxin Tan,Yuqi Bao,Sen Liao,Duoduo Wang,Qiaorong Ye,Chensi Zeng,Ni Tan
To improve the recognition ability and the controlled release of andrographolide (ADR), it is necessary to prepare a better intelligent drug delivery carrier. In this paper, the magnetic andrographolide imprinted polymer (ADR-MMIP) with pH/redox/light triple stimulus response was successfully prepared by reverse atom transfer radical polymerization (RATRP), where ADR was as the template, β-Cyclodextrin (β-CD) and 4-amino-4′-methacrylamide azobenzene (AMAB) as the bi-functional monomers , and both of N, N′-bis (acryloyl) cystamine (BAC) and ethylene glycol dimethacrylate (EGDMA) as cross-linkers. A series of adsorption experiments indicated that ADR-MMIP had excellent adsorption and selectivity capacities with the maximum adsorption capacity 41.43 mg g −1 and the factors α 4.256 and β 1.940 for the selectivity and relative selectivity, respectively. The adsorption mechanism of ADR-MMIP was investigated the multiphase adsorption with the chemical action on heterogeneous material surface by the linear fitting with the isothermal and kinetic adsorption models, and the in-vitro ADR release showed ADR-MMIP was sensitive to the triple-stimuli of pH/redox/light with the maximum cumulative drug release rate 91.53% when the above three conditions were applied concurrently. The above information demonstrated the ADR-MMIP should be of great application potential in the ADR extraction and isolation from mixture and the intelligent drug delivery system.
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JOURNAL OF CHROMATOGRAPHY A
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2022
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IF 4.601
Qianying Sheng,Ling Wang,Leyuan Zhang,Xue Wang,Shengxu Qian,Minbo Lan,Guangyan Qing,Xinmiao Liang
Natural products are rather complex samples containing a large number of compounds ranging from polar to nonpolar, small molecules to macromolecules , as well as numerous homologs/analogs with quite similar structures, which create great opportunities and treasures for discovery of bioactive drugs. For the purpose of better understanding the complex natural products and controlling their qualities, powerful analytical techniques for adequately separating chemical constituents and tracking potentially bioactive components are quite essential. Here, a design concept of bidirectional β-cyclodextrin (β-CD)–modified chromatographic stationary phase toward separation of bufadienolides extracted from toad is proposed. Bufadienolides could be divided into two classes: toad venom ligand (AAUBs) and toad toxin (AACBs) with remarkable differences in structures and polarity. The hydrophobic cavity of β-CD can encapsulate the steroid part of AACBs while the hydroxyls exposed on the β-CD surface have strong hydrophilic interactions with the arginine part of AACBs. Isothermal titration calorimetry and hydrogen nuclear magnetic resonance titration experiment further validate the rationality of this design. Furthermore, the β-CD-based stationary phase can be used as a hydrophilic material to construct a HILIC × RPLC 2D separation mode for the separation of AACBs, also works as a reverse phase material to construct a RPLC × RPLC 2D separation mode for the separation of AAUBs with good orthogonality. This study will open an avenue and provide a novel insight for high-efficiency two-dimensional separation of natural products.
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TALANTA
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2022
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IF 6.556
Xiaobin Zhou,Mengjun Wang,Junyang Chen,Xingguang Su
Developing high-performance nanozyme for the construction of high-sensitivity biosensor platform is of great significance. Herein, we have designed and synthesized a Cu/N co-doped carbon-based nanozyme by anchoring Cu atoms on two-dimensional carbon nanosheet (Cu/NC NS). The Cu/NC NS possessed ultra-high Cu loading, superior peroxidase-like catalytic activity, great stability and reusability. Significantly, the Cu/NC NS exhibited high affinity towards H 2 O 2 and 3,3′,5,5′-tetramethylbenzidine (TMB) under acidic condition, which could induce obvious color changes of TMB in the presence of low H 2 O 2 concentration. By integrating the Cu/NC NS with β-galactosidase (β-Gal) and galactose oxidase (Gal Ox), a multienzyme cascade colorimetric sensing system was established for the detection of lactose and β-Gal, which realizing the assay of lactose and β-Gal in the linear ranges of 0.1–1.4 mM and 0.025–0.2 U/mL, respectively. And the limit of detection for lactose and β-Gal were 0.03 mM and 0.01 U/mL, respectively. Furthermore, this work achieved the accurate detection of the content of lactose in milk and the activity of β-Gal in human serum, exploiting a novel application for nanozyme in biosensing.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2022
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IF 9.136
Dongli Li,Zhaoqiang Zhang,Liqin Zhou,Youquan Zhang,Zhenxia Zhao,Fang Shen,Xingzhen Qin,Kungang Chai,Hongbing Ji
To efficiently separate acetophenone (AP) and 1-phenyethanol (PE) from petrochemical by-product, we herein report a novel feasible strategy to fabricate β-cyclodextrin (β-CD) based core–shell microsphere, which retains the cage-type crystal structure of β-CD and superior affinity to AP over PE. Using triphenylmethane-4,4′,4′'-triisocyanate (TTI) as monomer, the polymeric TTI shell can grow in-situ on the surface of β-CD particles, giving the core–shell structured CD@PTTI. The unique β-CD core and polymeric TTI shell structure was verified by a series of characterization methods. CD@PTTI-3 exhibits both high AP uptake (ca. 2.07 mmol·g −1 ) and AP/PE selectivity (ca. 7.2), outperforming the conventional TTI cross-linked amorphous β-CD polymer (TTI-CDP). The adsorption behavior of CD@PTTI-3 on AP and PE single-component solutions was investigated by batch adsorption experiments. Based on the experimental and theoretical results, a mechanism of competitive adsorption of AP and PE on CD@PTTI-3 involving host–guest inclusion, hydrogen bonding and π-π stacking is proposed. Furthermore, intense competition between AP and PE on CD@PTTI-3 was observed in column breakthrough experiments, and CD@PTTI-3 could purify AP from 77.16% to 93.6% by a single adsorption–desorption process.
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TISSUE & CELL
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2022
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IF 2.586
Yuan Xu,Jian He,Jun He
Purpose The treatment of intervertebral disc degeneration is limited, cyanidin can protect chondrocytes from degeneration. This research investigated the effect of cyanidin on human nucleus pulposus cells (HNPC) metabolism and its mechanism. Methods HNPC were treated with cyanidin, XAV-939 (inhibitor of Wnt/β-catenin signaling) and SKL2001 (activator of Wnt/β-catenin signaling), and pressurized at 1, 3, and 30 atm. Quantitative real-time PCR and Western blot were used to assess the expressions of matrix metalloproteinase-3 (MMP-3), matrix metalloproteinase-13 (MMP-13), Collagen-II, Aggrecan , Wnt-3a and β-catenin in treated HNPC. Cell counting kit-8 was used to detect the HNPC cell viability . Radioisotope incorporation method was used to assess the proteoglycan synthesis rate of HNPC. The level of cell matrix was detected by toluidine blue staining. Results Proper hydrostatic pressure of 3 atm could elevate cell viability, proteoglycan synthesis and the level of cell matrix of HNPC, while high hydrostatic pressure of 30 atm reduced the above effects. Cyanidin and XAV-939 reversed the promoting effect of 30 atm pressure on Wnt/β-catenin signaling pathway and the inhibiting effect on cell viability, proteoglycan synthesis and the level of cell matrix. Subsequently, SKL2001 further reversed the function of cyanidin on HNPC. Conclusion Cyanidin attenuated the high hydrostatic pressure-induced degradation of cellular matrix of HNPC via blocking the Wnt/β-catenin signaling pathway. Our findings in this research provided a basis for in vitro experiment of cyanidin and a theoretical fundament on this disease.
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Drug Design Development and Therapy
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2022
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IF 4.319
Shanshan Jia,Pin Guo,Junhua Lu,Xujun Huang,Laming Deng,Yan Jin,Lanyan Zhao,Xiaofang Fan
Background Curcumol exhibits anti-inflammatory effect, but its effect on chronic asthma lacked research. Therefore, this study explored the role of curcumol in asthma.Methods A chronic asthmatic mice model was established by ovalbumin induction. After treatment with curcumol, airway resistance in mice was detected by forced oscillation technique. The histopathological features of airway tissues, pulmonary inflammation, and inflammation cell recruitment in the bronchoalveolar lavage fluid (BALF) of mice were detected by hematoxylin-eosin staining. Collagen deposition in the airways of mice was examined by Masson staining. The secretion of ovalbumin-IgE, IL-4, IL-5, IL-13 in mouse serum and VEGFA secretion in BALF were analyzed by ELISA. Finally, the expressions of β-catenin, Wnt5a, VEGFA, TGF-β1, Fibronectin, and MMP-9 in mice lung tissues were determined by Western blot or immunohistochemical.Results Curcumol attenuated airway hyperresponsiveness, airway remodeling, and pulmonary inflammation in chronic asthmatic mice. Curcumol relieved collagen deposition in airway tissues, inflammation cell recruitment in BALF, and reduced the up-regulation of serum ovalbumin-IgE, IL-4, IL-5, and IL-13 and BALF VEGFA in chronic asthmatic mice. In addition, curcumol attenuated the up-regulated expressions of β-catenin, Wnt5a, VEGFA, TGF-β1, Fibronectin, and MMP-9 in the lung tissues of chronic asthmatic mice, but curcumol treatment did not show such effects on healthy mice.Conclusion Our findings revealed that curcumol could ameliorate lung inflammation and airway remodeling by inhibiting the abnormal activation of the Wnt/β-catenin pathway in chronic asthmatic mice, indicating that curcumol could be used as a novel anti-asthma drug for basic and clinical research.
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Materials Today Bio
·
2022
·
IF 10.761
Xi He,Wenbin Liu,Yanling Liu,Kai Zhang,Yan Sun,Pengfei Lei,Yihe Hu
Bone nonunion or delayed union, caused by stripping or injuring of periosteum, is the most common sequelae of segmental bone defects. The preservation of periosteum, or the use of periosteal grafts, can significantly improve the integration of bone graft, speeding up the process of bone reconstruction. However, in most cases, periosteum cannot be preserved with bioactivity. Thus, it is pivotal to develop artificial periosteum. In this study, artificial periosteum of PLGA/MgO/Quercetin was prepared by electrospinning. PLGA/MgO/Quercetin membranes were shown to have a highly porous surface and microstructure, as observed by scanning electron microscopy. Along with excellent biocompatibility, PLGA/MgO/Quercetin membranes promoted cell proliferation and migration, as well as osteogenic differentiation of BMSCs (Bone marrow mesenchymal stem cells) in a dose-dependent manner through the activation of Wnt/β-Catenin pathway. The PLGA/MgO/Quercetin membranes, with an appropriate concentration of quercetin (<1 wt%), promoted EPCs (Endothelial progenitor cells) angiogenesis. In a subcutaneous implantation model and rat skull defect model, optimal osteogenesis and angiogenesis function were observed for the PLGA/20 wt% MgO/0.1 wt% Quercetin membrane. In conclusion, PLGA/MgO membranes, with an appropriate concentration of quercetin, show a variety of biological activities and are promising materials for the generation of artificial periosteum.
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JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
·
2022
·Vol. 381
·期 3
·257-265
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IF 4.402
Jian-Li Qiu,Guo-Feng Zhang,Yu-Na Chai,Xiao-Yan Han,Hai-Tao Zheng,Xiang-Feng Li,Fei Duan,Ling-Yan Chen
To investigate therapeutic target for ligustrazine during liver fibrosis in an ethanol-induced biliary atresia rat model and transforming growth factor-β (TGF-β) induced hepatic stellate cell activation cell model, and the underlying mechanism, a total of 30 rats were randomly assigned into five groups (n = 6 per group): control, sham, ethanol-induced biliary atresia model, model plus pirfenidone, and model plus ligustrazine groups. The liver changes were assessed using H&E and Masson staining and transmission electron microscopy. Expression of miR-145 and mRNA and protein levels of TGF-β/smads pathway-related proteins were detected. HSC-T6 cells were infected with LV-miR or rLV-miR-145 in the presence or absence of SMAD3 inhibitor SIS3 and treated with 2.5 ng/ml TGF-β1 and then with ligustrazine. Collected cells were subjected to detect the expression of miR-145 and mRNA and protein expression levels of TGF-β/smads pathway–related proteins. Ligustrazine rescued liver fibrogenesis and pathology for ethanol-caused bile duct injury, revealed by decreased α-smooth muscle actin and collagen I expression and liver tissue and cell morphology integrity. Further experiments showed that ligustrazine inhibited intrinsic and phosphorylated Smad2/3 protein expression and modification. Similar results were obtained in cells. In addition, ligustrazine altered miR-145 expression in both animal and cell models. Lentivirus mediated miR-145 overexpression and knockdown recombinant virus showed that miR-145 enhanced the TGF-β/Smad pathway, which led to hepatic stellate cell activation, and ligustrazine blocked this activation. This work validated that ligustrazine-regulated miR-145 mediated TGF-β/Smad signaling to inhibit the progression of liver fibrosis in a biliary atresia rat model and provided a new therapeutic strategy for liver fibrosis.
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Foods
·
2022
·Vol. 11
·期 18
·2720
·
IF 5.561
Yaping Dai,Xuli Lu,Ruyi Li,Yupo Cao,Wei Zhou,Jihua Li,Baodong Zheng
W/O/W emulsions can be used to encapsulate both hydrophobic and hydrophilic bioactive as nutritional products. However, studies on protein stabilized gel-like W/O/W emulsions have rarely been reported, compared to the liquid state multiple emulsions. The purpose of this study was to investigate the effect of different oil–water ratios on the stability of W/O/W emulgels fabricated with salt-soluble proteins (SSPs) ofSipunculus nudus. The physical stability, structural characteristics, rheological properties, and encapsulation stability of vitamin C and β-carotene of double emulgels were investigated. The addition of W/O primary emulsion was determined to be 10% after the characterization of the morphology of double emulsion. The results of microstructure and rheological properties showed that the stability of W/O/W emulgels increased with the increasing concentration of SSPs. Additionally, the encapsulation efficiency of vitamin C and β-carotene were more than 87%, and 99%, respectively, and still could maintain around 50% retention of the antioxidant capacity after storage for 28 days at 4 °C. The aforementioned findings demonstrate that stable W/O/W emulgels are a viable option for active ingredients with an improvement in shelf stability and protection of functional activity.Keywords:W/O/W emulgels;Sipunculus nudus;salt-soluble proteins;vitamin C;β-carotene;co-encapsulation
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